Rorug06G0423100

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000254
Physical Location & Seq
Reverse (-)
56141498 .. 56144897
3400 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0423100.1

Sequence Viewer

Length: 1104 bp
ATGGAAGCAATCAGGAAGATAAGAGATCAGGTCGCTAAACAGCAACAGGCTGTCTTCAAACAATTTGCTGGTGGCATATATGGGAGTTCGGATAATGTTGTCGCGGACGAAACAGAACTCCAACAGCATCAGAAACTCGAGAAGTTGTTCATATCAACTCGTGCCGGCAAGCATTACCAAAGGGATATTGTGCGTGGTGTGGAAAGTTATATTATAACTGGTTCAAAGCTAGTTGAAATAGGTACCAAGTTATCAGAAGATAGCAGGAAATATGGTGCTGAAAAAACTTGCACCACTGGTAACACATTATCCCGCGCTGCACTAAGTTTTGGAAGAGCTCGTGCCCAGATGGAAAAGGAGTGTGGAATTCTATTGAAAGCCCTTGGCACACAGGTTGCAGAGCCTTTAAGAGCGATGGTAGTGGGTGCACCCTTGGAGGATGCTCGACATCTTGCTCAACGATACAGTAGAATGCGACAAGAAGCTGAAGCTCAGGCTATCGATGTTTCCAGACGCCAAGTCAAAGTACGGGAATCCGCAGGTAATGTAGATATTATCATGAAACTAGAAGCTGCTGAAGCCAAACTTCAGGAGCTAAAGTCCAATGTTGCAATATTGGGAAAAGAAGCTGCTGCAGCAATGGCAGCTGTAGAAGGTCAACAACAGAGGTTGACTCTTCAGCGACTTATTGCCATGGTTGAAGCAGAGCGCAATTATCATCAGACTGTCCTCCAGATACTTGATCGGCTTGAAGGCGAGATGTTGTCAGAACACCAGCGAATCGAAGCATCCCCTAGTCCAGCTGCAGAGAATACTATGCCTCCACCACCCTCATATGAAGAGGTCAACCATGACTTTTCTCCTGAAACCCATGATGATTTAACAGACATCAGGGACTATTTCTTAGGAGAGGCTATGTACACATTTCAAGGTGTAACTGATGTGGAGTTGAGTTTGTCTTTCGGGGATTATGTTGTTGTCCGAAAGGTTTCAAACAATGGTTGGGCTGAAGGTGAAAGTAAGGGAAAAGCAGGTTGGTTCCCGTTTAACTACATAGAAAGGCGGGAACGTGTTCATGCGAGCAAGGTGGCTGCAGTCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

367

Amino Acids

40.96

Weight (kDa)

6.14

Isoelectric Point (pI)

40.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3_9 PF14604 305 - 353 4.5e-10 Variant SH3 domain
SH3_1 PF00018 305 - 348 1.8e-07 SH3 domain
SH3_2 PF07653 316 - 353 4.8e-07 Variant SH3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000254)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25671 FvH4_1g29782 FvH4_2g06731 FvH4_2g09413 FvH4_2g12061 FvH4_2g12671 FvH4_2g14202 FvH4_3g27901 FvH4_3g30492 FvH4_3g36953 FvH4_4g10141 FvH4_4g11162 FvH4_5g27995 FvH4_5g34262 FvH4_6g10681 FvH4_6g13733 FvH4_6g18164 FvH4_6g25491 FvH4_6g25791 FvH4_6g27023 FvH4_6g34321 FvH4_7g05070
pyrus_communis pycom04g06850
rosa_chinensis RchiOBHm_Chr1g0322281 RchiOBHm_Chr1g0334981 RchiOBHm_Chr1g0371181 RchiOBHm_Chr2g0125461 RchiOBHm_Chr2g0127141 RchiOBHm_Chr2g0128961 RchiOBHm_Chr2g0132921 RchiOBHm_Chr3g0486471 RchiOBHm_Chr3g0492911 RchiOBHm_Chr4g0412131 RchiOBHm_Chr4g0426941 RchiOBHm_Chr4g0437331 RchiOBHm_Chr5g0018821 RchiOBHm_Chr5g0040021 RchiOBHm_Chr5g0051041 RchiOBHm_Chr6g0266871 RchiOBHm_Chr6g0271761 RchiOBHm_Chr7g0200931 RchiOBHm_Chr7g0220091
rosa_multiflora Rmu_co7991344.1_g000001 Rmu_sc0000172.1_g000008 Rmu_sc0000194.1_g000010 Rmu_sc0000446.1_g000026 Rmu_sc0000496.1_g000011 Rmu_sc0000530.1_g000013 Rmu_sc0000706.1_g000044 Rmu_sc0000745.1_g000029 Rmu_sc0001301.1_g000027 Rmu_sc0001520.1_g000008 Rmu_sc0001966.1_g000025 Rmu_sc0001989.1_g000007 Rmu_sc0002239.1_g000002 Rmu_sc0002816.1_g000004 Rmu_sc0003458.1_g000001 Rmu_sc0003825.1_g000031 Rmu_sc0003919.1_g000002 Rmu_sc0004053.1_g000007 Rmu_sc0004161.1_g000008 Rmu_sc0004368.1_g000033 Rmu_sc0004383.1_g000002 Rmu_sc0004923.1_g000002 Rmu_sc0005065.1_g000001 Rmu_sc0005478.1_g000001 Rmu_sc0006847.1_g000031 Rmu_sc0009556.1_g000002 Rmu_sc0010783.1_g000007 Rmu_sc0011424.1_g000022 Rmu_sc0013205.1_g000002 Rmu_sc0017407.1_g000001 Rmu_sc0023757.1_g000002 Rmu_sc0032346.1_g000001 Rmu_ssc0000008.1_g000014 Rmu_ssc0000158.1_g000033 Rmu_ssc0000217.1_g000028
rosa_roxburghii Rroxscaffold_1G00000480 Rroxscaffold_1G00026050 Rroxscaffold_1G00026250 Rroxscaffold_2G00085120 Rroxscaffold_2G00119710 Rroxscaffold_2G00126610 Rroxscaffold_2G00129340 Rroxscaffold_3G00245550 Rroxscaffold_5G00340210 Rroxscaffold_5G00340330 Rroxscaffold_6G00397180 Rroxscaffold_7G00216160
rosa_rugosa Rorug01G0040000 Rorug01G0067400 Rorug01G0092300 Rorug01G0197000 Rorug01G0474200 Rorug01G0482500 Rorug01G0489300 Rorug02G0142900 Rorug02G0259000 Rorug02G0283200 Rorug02G0341400 Rorug02G0341600 Rorug02G0370900 Rorug02G0379700 Rorug02G0386100 Rorug02G0387300 Rorug03G0112600 Rorug03G0265600 Rorug03G0298800 Rorug04G0019500 Rorug05G0069900 Rorug05G0070500 Rorug05G0256800 Rorug05G0287300 Rorug05G0592000 Rorug06G0057800 Rorug06G0082000 Rorug06G0118900 Rorug06G0176500 Rorug06G0423100 Rorug07G0050100 Rorug07G0095100 Rorug07G0202100 Rorug07G0216600 Rorug07G0244300 Rorug07G0245500
rosa_samantha Rh1DG154800 Rh2CG613000 Rh6BG016600 Rh6BG475100 Rh6CG013200 Rh7AG443800
rosa_wichuraiana Rw0G021840 Rw4G023650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 215
Acc36I ACCTGC 2 cut(s) 530, 1022
Acc65I GGTACC 1 cut(s) 242
AccB1I GGYRCC 1 cut(s) 242
AccII CGCG 2 cut(s) 104, 315
AciI CCGC 4 cut(s) 104, 313, 537, 1063
AcsI RAATTY 1 cut(s) 366
AcuI CTGAAG 5 cut(s) 507, 572, 597, 662, 1029
AcyI GRCGYC 1 cut(s) 514
AfaI GTAC 3 cut(s) 244, 528, 920
AflIII ACRYGT 1 cut(s) 1069
AgsI TTSAA 8 cut(s) 58, 225, 236, 376, 701, 752, 929, 993
AhdI GACNNNNNGTC 1 cut(s) 518
AjuI GAANNNNNNNTTGG 2 cut(s) 1018, 1050
AluBI AGCT 9 cut(s) 229, 338, 485, 491, 572, 595, 629, 647, 803
AluI AGCT 9 cut(s) 229, 338, 485, 491, 572, 595, 629, 647, 803
Alw21I GWGCWC 2 cut(s) 340, 430
Alw44I GTGCAC 1 cut(s) 426
Ama87I CYCGRG 1 cut(s) 137
ApaLI GTGCAC 1 cut(s) 426
ApeKI GCWGC 8 cut(s) 317, 572, 629, 632, 635, 644, 803, 1091
ApoI RAATTY 1 cut(s) 366
Asp700I GAANNNNTTC 3 cut(s) 146, 988, 1071
Asp718I GGTACC 1 cut(s) 242
AspLEI GCGC 2 cut(s) 317, 711
AsuHPI GGTGA 1 cut(s) 1025
AvaI CYCGRG 1 cut(s) 137
BaeGI GKGCMC 2 cut(s) 346, 430
BanI GGYRCC 1 cut(s) 242
BanII GRGCYC 1 cut(s) 340
BauI CACGAG 2 cut(s) 159, 339
BbsI GAAGAC 1 cut(s) 46
Bbv12I GWGCWC 2 cut(s) 340, 430
BbvI GCAGC 8 cut(s) 304, 559, 616, 619, 647, 656, 790, 1078
BccI CCATC 2 cut(s) 343, 409
BfaI CTAG 3 cut(s) 230, 566, 795
BfmI CTRYAG 4 cut(s) 633, 648, 804, 1092
BfuAI ACCTGC 2 cut(s) 530, 1022
BisI GCNGC 8 cut(s) 318, 573, 630, 633, 636, 645, 804, 1092
BlsI GCNGC 8 cut(s) 319, 574, 631, 634, 637, 646, 805, 1093
BmeRI GACNNNNNGTC 1 cut(s) 518
BmeT110I CYCGRG 1 cut(s) 137
BmiI GGNNCC 2 cut(s) 244, 1040
BmsI GCATC 3 cut(s) 136, 430, 797
BpiI GAAGAC 1 cut(s) 46
BplI GAGNNNNNCTC 2 cut(s) 658, 690
BpmI CTGGAG 1 cut(s) 716
Bpu10I CCTNAGC 1 cut(s) 492
Bsa29I ATCGAT 1 cut(s) 501
BsaHI GRCGYC 1 cut(s) 514
BsaJI CCNNGG 3 cut(s) 382, 432, 693
BsaXI ACNNNNNCTCC 4 cut(s) 584, 614, 805, 835
Bse118I RCCGGY 1 cut(s) 164
Bse1I ACTGG 2 cut(s) 223, 301
Bse3DI GCAATG 1 cut(s) 645
BseCI ATCGAT 1 cut(s) 501
BseDI CCNNGG 3 cut(s) 382, 432, 693
BseGI GGATG 2 cut(s) 445, 788
BseMI GCAATG 1 cut(s) 645
BseMII CTCAG 1 cut(s) 506
BseNI ACTGG 2 cut(s) 223, 301
BseSI GKGCMC 2 cut(s) 346, 430
BseXI GCAGC 8 cut(s) 304, 559, 616, 619, 647, 656, 790, 1078
BsgI GTGCAG 1 cut(s) 303
Bsh1236I CGCG 2 cut(s) 104, 315
BshNI GGYRCC 1 cut(s) 242
BshVI ATCGAT 1 cut(s) 501
BsiHKAI GWGCWC 2 cut(s) 340, 430
BsiHKCI CYCGRG 1 cut(s) 137
BsiSI CCGG 1 cut(s) 165
BslFI GGGAC 1 cut(s) 908
BsmFI GGGAC 1 cut(s) 908
BsmI GAATGC 1 cut(s) 477
BsoBI CYCGRG 1 cut(s) 137
Bsp1286I GDGCHC 3 cut(s) 340, 346, 430
Bsp1407I TGTACA 1 cut(s) 918
Bsp143I GATC 2 cut(s) 25, 742
Bsp19I CCATGG 1 cut(s) 693
BspACI CCGC 4 cut(s) 104, 313, 537, 1063
BspCNI CTCAG 1 cut(s) 505
BspDI ATCGAT 1 cut(s) 501
BspFNI CGCG 2 cut(s) 104, 315
BspHI TCATGA 1 cut(s) 558
BspLI GGNNCC 2 cut(s) 244, 1040
BspMAI CTGCAG 3 cut(s) 637, 808, 1096
BspMI ACCTGC 2 cut(s) 530, 1022
BspQI GCTCTTC 1 cut(s) 328
BspT107I GGYRCC 1 cut(s) 242
BsrDI GCAATG 1 cut(s) 645
BsrFI RCCGGY 1 cut(s) 164
BsrGI TGTACA 1 cut(s) 918
BsrI ACTGG 2 cut(s) 223, 301
BssAI RCCGGY 1 cut(s) 164
BssECI CCNNGG 3 cut(s) 382, 432, 693
BssMI GATC 2 cut(s) 25, 742
BssNI GRCGYC 1 cut(s) 514
BssSI CACGAG 2 cut(s) 159, 339
BssT1I CCWWGG 3 cut(s) 382, 432, 693
Bst2BI CACGAG 2 cut(s) 159, 339
Bst4CI ACNGT 2 cut(s) 467, 727
Bst6I CTCTTC 3 cut(s) 328, 681, 834
BstACI GRCGYC 1 cut(s) 514
BstAUI TGTACA 1 cut(s) 918
BstC8I GCNNGC 3 cut(s) 166, 170, 1081
BstDEI CTNAG 3 cut(s) 323, 492, 904
BstDSI CCRYGG 1 cut(s) 693
BstF5I GGATG 2 cut(s) 445, 788
BstFNI CGCG 2 cut(s) 104, 315
BstHHI GCGC 2 cut(s) 317, 711
BstKTI GATC 2 cut(s) 28, 745
BstMBI GATC 2 cut(s) 25, 742
BstMWI GCNNNNNNNGC 4 cut(s) 578, 635, 641, 644
BstSFI CTRYAG 4 cut(s) 633, 648, 804, 1092
BstSLI GKGCMC 2 cut(s) 346, 430
BstUI CGCG 2 cut(s) 104, 315
BstV1I GCAGC 8 cut(s) 304, 559, 616, 619, 647, 656, 790, 1078
BstV2I GAAGAC 1 cut(s) 46
Bsu15I ATCGAT 1 cut(s) 501
BsuTUI ATCGAT 1 cut(s) 501
BtgI CCRYGG 1 cut(s) 693
BtgZI GCGATG 1 cut(s) 428
BtsCI GGATG 2 cut(s) 445, 788
BtsIMutI CAGTG 1 cut(s) 294
BveI ACCTGC 2 cut(s) 530, 1022
Cac8I GCNNGC 3 cut(s) 166, 170, 1081
CciI TCATGA 1 cut(s) 558
CfoI GCGC 2 cut(s) 317, 711
Cfr10I RCCGGY 1 cut(s) 164
ClaI ATCGAT 1 cut(s) 501
CseI GACGC 1 cut(s) 522
Csp6I GTAC 3 cut(s) 243, 527, 919
CviAII CATG 5 cut(s) 559, 694, 851, 872, 1076
CviQI GTAC 3 cut(s) 243, 527, 919
DdeI CTNAG 3 cut(s) 323, 492, 904
DpnI GATC 2 cut(s) 27, 744
DpnII GATC 2 cut(s) 25, 742
DriI GACNNNNNGTC 1 cut(s) 518
Eam1104I CTCTTC 3 cut(s) 328, 681, 834
Eam1105I GACNNNNNGTC 1 cut(s) 518
EarI CTCTTC 3 cut(s) 328, 681, 834
Ecl136II GAGCTC 1 cut(s) 338
Eco130I CCWWGG 3 cut(s) 382, 432, 693
Eco24I GRGCYC 1 cut(s) 340
Eco53kI GAGCTC 1 cut(s) 338
Eco57I CTGAAG 5 cut(s) 507, 572, 597, 662, 1029
Eco88I CYCGRG 1 cut(s) 137
EcoICRI GAGCTC 1 cut(s) 338
EcoRI GAATTC 1 cut(s) 366
EcoT14I CCWWGG 3 cut(s) 382, 432, 693
EcoT38I GRGCYC 1 cut(s) 340
ErhI CCWWGG 3 cut(s) 382, 432, 693
FaeI CATG 5 cut(s) 562, 697, 854, 875, 1079
FalI AAGNNNNNCTT 2 cut(s) 570, 602
FaqI GGGAC 1 cut(s) 908
FatI CATG 5 cut(s) 558, 693, 850, 871, 1075
FauI CCCGC 2 cut(s) 320, 1056
FauNDI CATATG 1 cut(s) 835
Fnu4HI GCNGC 8 cut(s) 318, 573, 630, 633, 636, 645, 804, 1092
FokI GGATG 2 cut(s) 452, 775
FriOI GRGCYC 1 cut(s) 340
Fsp4HI GCNGC 8 cut(s) 318, 573, 630, 633, 636, 645, 804, 1092
FspBI CTAG 3 cut(s) 230, 566, 795
GlaI GCGC 2 cut(s) 316, 710
GluI GCNGC 8 cut(s) 318, 573, 630, 633, 636, 645, 804, 1092
GsuI CTGGAG 1 cut(s) 716
HapII CCGG 1 cut(s) 165
HgaI GACGC 1 cut(s) 522
HhaI GCGC 2 cut(s) 317, 711
Hin1I GRCGYC 1 cut(s) 514
Hin1II CATG 5 cut(s) 562, 697, 854, 875, 1079
Hin6I GCGC 2 cut(s) 315, 709
HinP1I GCGC 2 cut(s) 315, 709
HincII GTYRAC 3 cut(s) 659, 672, 847
HindII GTYRAC 3 cut(s) 659, 672, 847
HinfI GANTC 3 cut(s) 533, 673, 780
HpaII CCGG 1 cut(s) 165
HphI GGTGA 1 cut(s) 1025
Hpy166II GTNNAC 5 cut(s) 428, 659, 672, 847, 921
Hpy188I TCNGA 6 cut(s) 91, 132, 256, 723, 769, 983
Hpy188III TCNNGA 7 cut(s) 13, 139, 510, 559, 590, 733, 863
Hpy8I GTNNAC 5 cut(s) 428, 659, 672, 847, 921
HpyAV CCTTC 3 cut(s) 647, 746, 1004
HpyCH4III ACNGT 2 cut(s) 467, 727
HpyCH4IV ACGT 1 cut(s) 1069
HpyCH4V TGCA 8 cut(s) 291, 320, 398, 428, 611, 635, 806, 1094
HpyF10VI GCNNNNNNNGC 4 cut(s) 578, 635, 641, 644
HpyF3I CTNAG 3 cut(s) 323, 492, 904
HpySE526I ACGT 1 cut(s) 1069
Hsp92I GRCGYC 1 cut(s) 514
Hsp92II CATG 5 cut(s) 562, 697, 854, 875, 1079
HspAI GCGC 2 cut(s) 315, 709
KpnI GGTACC 1 cut(s) 246
KroI GCCGGC 1 cut(s) 164
KroNI GCCGGC 1 cut(s) 166
Kzo9I GATC 2 cut(s) 25, 742
LguI GCTCTTC 1 cut(s) 328
LmnI GCTCC 1 cut(s) 592
Lsp1109I GCAGC 8 cut(s) 304, 559, 616, 619, 647, 656, 790, 1078
LweI GCATC 3 cut(s) 136, 430, 797
MaeI CTAG 3 cut(s) 230, 566, 795
MaeII ACGT 1 cut(s) 1069
MaeIII GTNAC 2 cut(s) 299, 934
MalI GATC 2 cut(s) 27, 744
MboI GATC 2 cut(s) 25, 742
MboII GAAGA 6 cut(s) 28, 46, 269, 345, 668, 851
MhlI GDGCHC 3 cut(s) 340, 346, 430
MluCI AATT 3 cut(s) 62, 366, 712
MlyI GAGTC 1 cut(s) 667
MmeI TCCRAC 1 cut(s) 145
MnlI CCTC 7 cut(s) 430, 660, 740, 831, 835, 841, 904
MroNI GCCGGC 1 cut(s) 164
MroXI GAANNNNTTC 3 cut(s) 146, 988, 1071
MseI TTAA 4 cut(s) 407, 881, 1047, 1102
MspA1I CMGCKG 2 cut(s) 647, 803
MspI CCGG 1 cut(s) 165
Mva1269I GAATGC 1 cut(s) 477
MvnI CGCG 2 cut(s) 104, 315
MwoI GCNNNNNNNGC 4 cut(s) 578, 635, 641, 644
NaeI GCCGGC 1 cut(s) 166
NcoI CCATGG 1 cut(s) 693
NdeI CATATG 1 cut(s) 835
NdeII GATC 2 cut(s) 25, 742
NgoMIV GCCGGC 1 cut(s) 164
NlaIII CATG 5 cut(s) 562, 697, 854, 875, 1079
NlaIV GGNNCC 2 cut(s) 244, 1040
PaeR7I CTCGAG 1 cut(s) 137
PagI TCATGA 1 cut(s) 558
PciSI GCTCTTC 1 cut(s) 328
PctI GAATGC 1 cut(s) 477
PdiI GCCGGC 1 cut(s) 166
PdmI GAANNNNTTC 3 cut(s) 146, 988, 1071
PfeI GAWTC 2 cut(s) 533, 780
PkrI GCNGC 8 cut(s) 319, 574, 631, 634, 637, 646, 805, 1093
PleI GAGTC 1 cut(s) 667
PpsI GAGTC 1 cut(s) 667
PsiI TTATAA 1 cut(s) 215
Psp124BI GAGCTC 1 cut(s) 340
PspN4I GGNNCC 2 cut(s) 244, 1040
PstI CTGCAG 3 cut(s) 637, 808, 1096
PvuII CAGCTG 2 cut(s) 647, 803
RsaI GTAC 3 cut(s) 244, 528, 920
RsaNI GTAC 3 cut(s) 243, 527, 919
SacI GAGCTC 1 cut(s) 340
SapI GCTCTTC 1 cut(s) 328
SaqAI TTAA 4 cut(s) 407, 881, 1047, 1102
SatI GCNGC 8 cut(s) 318, 573, 630, 633, 636, 645, 804, 1092
Sau3AI GATC 2 cut(s) 25, 742
SchI GAGTC 1 cut(s) 667
SduI GDGCHC 3 cut(s) 340, 346, 430
SfaNI GCATC 3 cut(s) 136, 430, 797
SfcI CTRYAG 4 cut(s) 633, 648, 804, 1092
Sfr274I CTCGAG 1 cut(s) 137
SlaI CTCGAG 1 cut(s) 137
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
Sse9I AATT 3 cut(s) 62, 366, 712
SsiI CCGC 4 cut(s) 104, 313, 537, 1063
SspI AATATT 1 cut(s) 615
SspMI CTAG 3 cut(s) 230, 566, 795
SstI GAGCTC 1 cut(s) 340
StyI CCWWGG 3 cut(s) 382, 432, 693
TaaI ACNGT 2 cut(s) 467, 727
TaiI ACGT 1 cut(s) 1072
TaqI TCGA 4 cut(s) 138, 445, 501, 783
TasI AATT 3 cut(s) 62, 366, 712
TatI WGTACW 1 cut(s) 918
TfiI GAWTC 2 cut(s) 533, 780
Tru1I TTAA 4 cut(s) 407, 881, 1047, 1102
Tru9I TTAA 4 cut(s) 407, 881, 1047, 1102
TscAI CASTG 1 cut(s) 301
TseI GCWGC 8 cut(s) 317, 572, 629, 632, 635, 644, 803, 1091
TspDTI ATGAA 4 cut(s) 139, 575, 852, 1064
TspRI CASTG 1 cut(s) 301
VneI GTGCAC 1 cut(s) 426
XapI RAATTY 1 cut(s) 366
XhoI CTCGAG 1 cut(s) 137
XmnI GAANNNNTTC 3 cut(s) 146, 988, 1071
XspI CTAG 3 cut(s) 230, 566, 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.