Rmu_sc0001520.1_g000008

Ribonuclease H

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001520.1
OG0000254
Physical Location & Seq
Forward (+)
37508 .. 40786
3279 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001520.1_g000008.1.cds

Sequence Viewer

Length: 888 bp
ctgcaccgccttgctgcgccgcacgcagtggatatcgctgctagagaacaccacagggacgaccaaacggaggaggcgaaccagctgaccaagtttcacggccatagaccgccgcagttcgtcggaaaagccgggtcgggtcaggttagacggaaaactcagacattgaagacaagttatcaacctcctactgactgctcaagcacttggagaagcatttctcatggtgctaagctgctaaggaaatgcctaaaatggcgtgtgggtgatgggagctctattaagttttggcttgatcattggctctttccctcatccctcatctgtttggctcttcctactgctgccattaacatcaatgcaaccatcaatagcttttggactgatgagggctggaacctggatctcctctcgtccatggtcccaggtgatattgtcaatcaaattatcaatatccctactggttttgatcattgtggttgtgattctttgatttggggagccacatctaatggtgccttcacagtcaagtctgcctataattctagttttgagttttccaacccccaaaattctcaattggctgctgtttggaaaagcaatgttcctcctaaactgaaaaccttcctgtgggctgctctccataagaagctgctaactaatgttcaaagggctagaagaggttttacctcctcctccttgtgccctatctgcaaggctgatgttgaaaccctcatccatttgttcagggtctatcctagatctcttgctatttggaatgcttttcttaaacctggggctatccttaactccttctccctagactggaatggttggattgctgctcaactacactgtcagacactgattcagaataatattaaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

295

Amino Acids

33.0

Weight (kDa)

9.51

Isoelectric Point (pI)

34.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000254)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25671 FvH4_1g29782 FvH4_2g06731 FvH4_2g09413 FvH4_2g12061 FvH4_2g12671 FvH4_2g14202 FvH4_3g27901 FvH4_3g30492 FvH4_3g36953 FvH4_4g10141 FvH4_4g11162 FvH4_5g27995 FvH4_5g34262 FvH4_6g10681 FvH4_6g13733 FvH4_6g18164 FvH4_6g25491 FvH4_6g25791 FvH4_6g27023 FvH4_6g34321 FvH4_7g05070
pyrus_communis pycom04g06850
rosa_chinensis RchiOBHm_Chr1g0322281 RchiOBHm_Chr1g0334981 RchiOBHm_Chr1g0371181 RchiOBHm_Chr2g0125461 RchiOBHm_Chr2g0127141 RchiOBHm_Chr2g0128961 RchiOBHm_Chr2g0132921 RchiOBHm_Chr3g0486471 RchiOBHm_Chr3g0492911 RchiOBHm_Chr4g0412131 RchiOBHm_Chr4g0426941 RchiOBHm_Chr4g0437331 RchiOBHm_Chr5g0018821 RchiOBHm_Chr5g0040021 RchiOBHm_Chr5g0051041 RchiOBHm_Chr6g0266871 RchiOBHm_Chr6g0271761 RchiOBHm_Chr7g0200931 RchiOBHm_Chr7g0220091
rosa_multiflora Rmu_co7991344.1_g000001 Rmu_sc0000172.1_g000008 Rmu_sc0000194.1_g000010 Rmu_sc0000446.1_g000026 Rmu_sc0000496.1_g000011 Rmu_sc0000530.1_g000013 Rmu_sc0000706.1_g000044 Rmu_sc0000745.1_g000029 Rmu_sc0001301.1_g000027 Rmu_sc0001520.1_g000008 Rmu_sc0001966.1_g000025 Rmu_sc0001989.1_g000007 Rmu_sc0002239.1_g000002 Rmu_sc0002816.1_g000004 Rmu_sc0003458.1_g000001 Rmu_sc0003825.1_g000031 Rmu_sc0003919.1_g000002 Rmu_sc0004053.1_g000007 Rmu_sc0004161.1_g000008 Rmu_sc0004368.1_g000033 Rmu_sc0004383.1_g000002 Rmu_sc0004923.1_g000002 Rmu_sc0005065.1_g000001 Rmu_sc0005478.1_g000001 Rmu_sc0006847.1_g000031 Rmu_sc0009556.1_g000002 Rmu_sc0010783.1_g000007 Rmu_sc0011424.1_g000022 Rmu_sc0013205.1_g000002 Rmu_sc0017407.1_g000001 Rmu_sc0023757.1_g000002 Rmu_sc0032346.1_g000001 Rmu_ssc0000008.1_g000014 Rmu_ssc0000158.1_g000033 Rmu_ssc0000217.1_g000028
rosa_roxburghii Rroxscaffold_1G00000480 Rroxscaffold_1G00026050 Rroxscaffold_1G00026250 Rroxscaffold_2G00085120 Rroxscaffold_2G00119710 Rroxscaffold_2G00126610 Rroxscaffold_2G00129340 Rroxscaffold_3G00245550 Rroxscaffold_5G00340210 Rroxscaffold_5G00340330 Rroxscaffold_6G00397180 Rroxscaffold_7G00216160
rosa_rugosa Rorug01G0040000 Rorug01G0067400 Rorug01G0092300 Rorug01G0197000 Rorug01G0474200 Rorug01G0482500 Rorug01G0489300 Rorug02G0142900 Rorug02G0259000 Rorug02G0283200 Rorug02G0341400 Rorug02G0341600 Rorug02G0370900 Rorug02G0379700 Rorug02G0386100 Rorug02G0387300 Rorug03G0112600 Rorug03G0265600 Rorug03G0298800 Rorug04G0019500 Rorug05G0069900 Rorug05G0070500 Rorug05G0256800 Rorug05G0287300 Rorug05G0592000 Rorug06G0057800 Rorug06G0082000 Rorug06G0118900 Rorug06G0176500 Rorug06G0423100 Rorug07G0050100 Rorug07G0095100 Rorug07G0202100 Rorug07G0216600 Rorug07G0244300 Rorug07G0245500
rosa_samantha Rh1DG154800 Rh2CG613000 Rh6BG016600 Rh6BG475100 Rh6CG013200 Rh7AG443800
rosa_wichuraiana Rw0G021840 Rw4G023650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 515
AciI CCGC 4 cut(s) 7, 20, 110, 113
AclWI GGATC 1 cut(s) 411
AcoI YGGCCR 1 cut(s) 100
AcsI RAATTY 1 cut(s) 571
AdeI CACNNNGTG 1 cut(s) 28
AfiI CCNNNNNNNGG 3 cut(s) 70, 630, 825
AgsI TTSAA 3 cut(s) 169, 668, 728
AjnI CCWGG 3 cut(s) 399, 424, 793
AluBI AGCT 5 cut(s) 85, 235, 276, 375, 652
AluI AGCT 5 cut(s) 85, 235, 276, 375, 652
Alw21I GWGCWC 1 cut(s) 278
AlwI GGATC 1 cut(s) 411
AlwNI CAGNNNCTG 1 cut(s) 865
AoxI GGCC 1 cut(s) 100
ApeKI GCWGC 8 cut(s) 14, 38, 235, 344, 584, 635, 652, 842
ApoI RAATTY 1 cut(s) 571
Asp700I GAANNNNTTC 2 cut(s) 217, 623
AspLEI GCGC 1 cut(s) 19
AspS9I GGNCC 1 cut(s) 421
AsuC2I CCSGG 1 cut(s) 133
AsuHPI GGTGA 2 cut(s) 278, 440
AvaII GGWCC 1 cut(s) 421
BaeGI GKGCMC 1 cut(s) 707
BanI GGYRCC 1 cut(s) 515
BanII GRGCYC 1 cut(s) 278
BarI GAAGNNNNNNTAC 2 cut(s) 670, 702
BbsI GAAGAC 1 cut(s) 176
Bbv12I GWGCWC 1 cut(s) 278
BbvI GCAGC 7 cut(s) 25, 222, 331, 571, 622, 639, 829
BccI CCATC 2 cut(s) 263, 374
BceAI ACGGC 1 cut(s) 115
BciT130I CCWGG 3 cut(s) 401, 426, 795
BclI TGATCA 2 cut(s) 295, 469
BcnI CCSGG 1 cut(s) 133
BfaI CTAG 5 cut(s) 42, 546, 675, 759, 821
BglII AGATCT 1 cut(s) 761
BlpI GCTNAGC 1 cut(s) 231
Bme1390I CCNGG 4 cut(s) 133, 401, 426, 795
Bme18I GGWCC 1 cut(s) 421
BmgT120I GGNCC 1 cut(s) 421
BmiI GGNNCC 4 cut(s) 398, 423, 502, 517
BmrFI CCNGG 4 cut(s) 133, 401, 426, 795
BpiI GAAGAC 1 cut(s) 176
Bpu10I CCTNAGC 1 cut(s) 239
Bpu1102I GCTNAGC 1 cut(s) 231
BpuEI CTTGAG 1 cut(s) 184
BpuMI CCSGG 1 cut(s) 133
BsaJI CCNNGG 3 cut(s) 417, 424, 794
BsaXI ACNNNNNCTCC 2 cut(s) 800, 830
Bsc4I CCNNNNNNNGG 3 cut(s) 70, 630, 825
Bse1I ACTGG 2 cut(s) 466, 830
Bse3DI GCAATG 1 cut(s) 607
BseBI CCWGG 3 cut(s) 401, 426, 795
BseDI CCNNGG 3 cut(s) 417, 424, 794
BseGI GGATG 2 cut(s) 314, 735
BseLI CCNNNNNNNGG 3 cut(s) 70, 630, 825
BseMI GCAATG 1 cut(s) 607
BseMII CTCAG 1 cut(s) 173
BseNI ACTGG 2 cut(s) 466, 830
BseRI GAGGAG 4 cut(s) 86, 398, 682, 685
BseSI GKGCMC 1 cut(s) 707
BseXI GCAGC 7 cut(s) 25, 222, 331, 571, 622, 639, 829
BshFI GGCC 1 cut(s) 102
BshNI GGYRCC 1 cut(s) 515
BsiHKAI GWGCWC 1 cut(s) 278
BsiSI CCGG 1 cut(s) 132
BslFI GGGAC 2 cut(s) 71, 407
BslI CCNNNNNNNGG 3 cut(s) 70, 630, 825
BsmFI GGGAC 2 cut(s) 71, 407
BsmI GAATGC 1 cut(s) 784
BsnI GGCC 1 cut(s) 102
Bsp1286I GDGCHC 2 cut(s) 278, 707
Bsp143I GATC 4 cut(s) 295, 403, 469, 761
Bsp1720I GCTNAGC 1 cut(s) 231
Bsp19I CCATGG 1 cut(s) 417
BspACI CCGC 4 cut(s) 7, 20, 110, 113
BspANI GGCC 1 cut(s) 102
BspCNI CTCAG 1 cut(s) 172
BspLI GGNNCC 4 cut(s) 398, 423, 502, 517
BspPI GGATC 1 cut(s) 411
BspQI GCTCTTC 1 cut(s) 339
BspT107I GGYRCC 1 cut(s) 515
BsrDI GCAATG 1 cut(s) 607
BsrI ACTGG 2 cut(s) 466, 830
BssECI CCNNGG 3 cut(s) 417, 424, 794
BssMI GATC 4 cut(s) 295, 403, 469, 761
BssT1I CCWWGG 1 cut(s) 417
Bst2UI CCWGG 3 cut(s) 401, 426, 795
Bst4CI ACNGT 2 cut(s) 526, 857
Bst6I CTCTTC 2 cut(s) 339, 673
BstC8I GCNNGC 1 cut(s) 24
BstDEI CTNAG 3 cut(s) 159, 231, 239
BstDSI CCRYGG 1 cut(s) 417
BstF5I GGATG 2 cut(s) 314, 735
BstHHI GCGC 1 cut(s) 19
BstKTI GATC 4 cut(s) 298, 406, 472, 764
BstMBI GATC 4 cut(s) 295, 403, 469, 761
BstMWI GCNNNNNNNGC 2 cut(s) 23, 711
BstNI CCWGG 3 cut(s) 401, 426, 795
BstSCI CCNGG 4 cut(s) 131, 399, 424, 793
BstSLI GKGCMC 1 cut(s) 707
BstV1I GCAGC 7 cut(s) 25, 222, 331, 571, 622, 639, 829
BstV2I GAAGAC 1 cut(s) 176
BstX2I RGATCY 2 cut(s) 403, 761
BstYI RGATCY 2 cut(s) 403, 761
BsuRI GGCC 1 cut(s) 102
BtgI CCRYGG 1 cut(s) 417
BtsCI GGATG 2 cut(s) 314, 735
BtsI GCAGTG 1 cut(s) 33
BtsIMutI CAGTG 3 cut(s) 33, 853, 863
Cac8I GCNNGC 1 cut(s) 24
CaiI CAGNNNCTG 1 cut(s) 865
CfoI GCGC 1 cut(s) 19
Cfr13I GGNCC 1 cut(s) 421
CviAII CATG 2 cut(s) 224, 418
DdeI CTNAG 3 cut(s) 159, 231, 239
DpnI GATC 4 cut(s) 297, 405, 471, 763
DpnII GATC 4 cut(s) 295, 403, 469, 761
DraIII CACNNNGTG 1 cut(s) 28
EaeI YGGCCR 1 cut(s) 100
Eam1104I CTCTTC 2 cut(s) 339, 673
EarI CTCTTC 2 cut(s) 339, 673
Ecl136II GAGCTC 1 cut(s) 276
Eco130I CCWWGG 1 cut(s) 417
Eco24I GRGCYC 1 cut(s) 278
Eco32I GATATC 1 cut(s) 34
Eco47I GGWCC 1 cut(s) 421
Eco53kI GAGCTC 1 cut(s) 276
EcoICRI GAGCTC 1 cut(s) 276
EcoRII CCWGG 3 cut(s) 399, 424, 793
EcoRV GATATC 1 cut(s) 34
EcoT14I CCWWGG 1 cut(s) 417
EcoT38I GRGCYC 1 cut(s) 278
ErhI CCWWGG 1 cut(s) 417
FaeI CATG 2 cut(s) 227, 421
FaiI YATR 5 cut(s) 105, 225, 419, 540, 645
FaqI GGGAC 2 cut(s) 71, 407
FatI CATG 2 cut(s) 223, 417
FbaI TGATCA 2 cut(s) 295, 469
FokI GGATG 2 cut(s) 301, 722
FriOI GRGCYC 1 cut(s) 278
FspBI CTAG 5 cut(s) 42, 546, 675, 759, 821
GlaI GCGC 1 cut(s) 18
HaeIII GGCC 1 cut(s) 102
HapII CCGG 1 cut(s) 132
HhaI GCGC 1 cut(s) 19
Hin1II CATG 2 cut(s) 227, 421
Hin6I GCGC 1 cut(s) 17
HinP1I GCGC 1 cut(s) 17
HinfI GANTC 2 cut(s) 485, 868
HpaII CCGG 1 cut(s) 132
HphI GGTGA 2 cut(s) 278, 440
Hpy188I TCNGA 4 cut(s) 125, 162, 861, 873
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 3 cut(s) 529, 634, 823
HpyCH4III ACNGT 2 cut(s) 526, 857
HpyCH4V TGCA 3 cut(s) 4, 362, 714
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 711
HpyF3I CTNAG 3 cut(s) 159, 231, 239
Hsp92II CATG 2 cut(s) 227, 421
HspAI GCGC 1 cut(s) 17
Ksp22I TGATCA 2 cut(s) 295, 469
Kzo9I GATC 4 cut(s) 295, 403, 469, 761
LguI GCTCTTC 1 cut(s) 339
LmnI GCTCC 2 cut(s) 273, 500
Lsp1109I GCAGC 7 cut(s) 25, 222, 331, 571, 622, 639, 829
MaeI CTAG 5 cut(s) 42, 546, 675, 759, 821
MalI GATC 4 cut(s) 297, 405, 471, 763
MboI GATC 4 cut(s) 295, 403, 469, 761
MboII GAAGA 3 cut(s) 181, 326, 690
MfeI CAATTG 1 cut(s) 578
MflI RGATCY 2 cut(s) 403, 761
MhlI GDGCHC 2 cut(s) 278, 707
MluCI AATT 4 cut(s) 444, 541, 571, 578
MmeI TCCRAC 3 cut(s) 103, 585, 815
MroXI GAANNNNTTC 2 cut(s) 217, 623
MseI TTAA 5 cut(s) 282, 351, 789, 807, 882
MspA1I CMGCKG 1 cut(s) 85
MspI CCGG 1 cut(s) 132
MspR9I CCNGG 4 cut(s) 133, 401, 426, 795
MunI CAATTG 1 cut(s) 578
Mva1269I GAATGC 1 cut(s) 784
MvaI CCWGG 3 cut(s) 401, 426, 795
MwoI GCNNNNNNNGC 2 cut(s) 23, 711
NciI CCSGG 1 cut(s) 133
NcoI CCATGG 1 cut(s) 417
NdeII GATC 4 cut(s) 295, 403, 469, 761
NlaIII CATG 2 cut(s) 227, 421
NlaIV GGNNCC 4 cut(s) 398, 423, 502, 517
PciSI GCTCTTC 1 cut(s) 339
PcsI WCGNNNNNNNCGW 1 cut(s) 74
PctI GAATGC 1 cut(s) 784
PdmI GAANNNNTTC 2 cut(s) 217, 623
PfeI GAWTC 2 cut(s) 485, 868
Psp124BI GAGCTC 1 cut(s) 278
Psp6I CCWGG 3 cut(s) 399, 424, 793
PspGI CCWGG 3 cut(s) 399, 424, 793
PspN4I GGNNCC 4 cut(s) 398, 423, 502, 517
PspPI GGNCC 1 cut(s) 421
PstNI CAGNNNCTG 1 cut(s) 865
PsuI RGATCY 2 cut(s) 403, 761
PvuII CAGCTG 1 cut(s) 85
SacI GAGCTC 1 cut(s) 278
SapI GCTCTTC 1 cut(s) 339
SaqAI TTAA 5 cut(s) 282, 351, 789, 807, 882
Sau3AI GATC 4 cut(s) 295, 403, 469, 761
Sau96I GGNCC 1 cut(s) 421
ScrFI CCNGG 4 cut(s) 133, 401, 426, 795
SduI GDGCHC 2 cut(s) 278, 707
SinI GGWCC 1 cut(s) 421
SmlI CTYRAG 1 cut(s) 199
SmoI CTYRAG 1 cut(s) 199
Sse9I AATT 4 cut(s) 444, 541, 571, 578
SsiI CCGC 4 cut(s) 7, 20, 110, 113
SspI AATATT 1 cut(s) 880
SspMI CTAG 5 cut(s) 42, 546, 675, 759, 821
SstI GAGCTC 1 cut(s) 278
StyD4I CCNGG 4 cut(s) 131, 399, 424, 793
StyI CCWWGG 1 cut(s) 417
TaaI ACNGT 2 cut(s) 526, 857
TasI AATT 4 cut(s) 444, 541, 571, 578
TauI GCSGC 2 cut(s) 22, 115
TfiI GAWTC 2 cut(s) 485, 868
Tru1I TTAA 5 cut(s) 282, 351, 789, 807, 882
Tru9I TTAA 5 cut(s) 282, 351, 789, 807, 882
TscAI CASTG 3 cut(s) 33, 860, 870
TseI GCWGC 8 cut(s) 14, 38, 235, 344, 584, 635, 652, 842
TspGWI ACGGA 2 cut(s) 83, 166
TspRI CASTG 3 cut(s) 33, 860, 870
VpaK11BI GGWCC 1 cut(s) 421
XapI RAATTY 1 cut(s) 571
XmnI GAANNNNTTC 2 cut(s) 217, 623
XspI CTAG 5 cut(s) 42, 546, 675, 759, 821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.