Rorug01G0067400

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000254
Physical Location & Seq
Reverse (-)
10957784 .. 10967999
10216 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0067400.1

Sequence Viewer

Length: 4452 bp
ATGGAGAACCTTGTGGCAGGGGGTGCTATAGGAGTACCATTTATTGTGCTGTATGAAGTCATTAAAGAAGCGATGGCCAAGAGTACGATGTTTAAACCCGTGCTTGTAGAGCTGTATTCGATAATGAACCATTTGGAACCACTGGTGGAGGAGATAAGCAAGTATGATAGAGAGTTAAATCGTCGAGGGAATGAGCTTAAAGACTTCAAAACACAAATGGAGAAGGGCATCGAGCTTATTCAGAAGTGCTCGAATCTTAATCGGTCACAAATCTACAAAAAGTATAGATACAGCAACCAACTTCTTGAATTTCAGAAGTTTCTTCAGAATCTGGTATCTATTCTGTCAGTGCAACAAGCAAGGGATGTGAAGGAGAAGTTGATTGCAACGAAAGACGAGATGAACAATAGTATGAAGGACCCCTTGGATGAGCTCAGAAATATAGAGGTGAAGCACCAAACAGACTTTGAAGTACCTGAGCTTCATGCGTTTCCAGTTGGATTGGATCTGCCTCTCAGAGAATTAAAGATGAAGCTTCTTAAGAATGATAAAGTGTCAATGCTTGTGCTCACTGCTCCAGGAGGATGCGGAAAAACCACATTGGCGACAATGTTTTGTCAAGATGAGCAAGTCAAAGATATATTCAAGGACAATATCTTCTTTTTCAATGTTTCAAAGATGTCGAATTTCAACCTCATTGTACAACGACTACAGCAACGCAAGGGTACCGAAGTACCTACTTTCGAAGACGAAGCAACTGCCCTCAAGTGGCTGCATAAATTTCTGGGGGAAGGACAGACACCTGTACTGTTGGTCCTGGATGACGTTTGGCCGGAATCAAGATCTCTTCTTGAAAAGTTTGATGAAGTGAAGGTGCCAAATTTTAAGATTTTGGTGACATCAAGATCACATTTTCCAGGATATGGTTCTCTACATCGTCTAAATTATTTATTGGATGATGAAGATGCAATGACTCTTTTTCGCCATTCAGCCTCCCTGGGAGATGAGAGCTCTAATGTTCGATGGAACAGTCTTGCTGCAAAGATAGTAAAGCACTGTAAGGGATATCCACTTGCCATTGAAGTGGTTGGAAGATCACTTCGCGGAAAATCGTTTGAATTCTGGCTTAAAAGTGCAGGGCAGTTGTCTAAAGGTGATTCTCTTATTGATTCTGAAATTGAAGTGCCTCTTGCGCTTCAAAGTAGCTTTGATGTTTTGGATGAAAACATCAAGGAATGTTTTGTAGACCTTGGTTCATTTCCTGAAGACCAAAAAATCCCAGTTGCTGCCCTCATTGATATGTGGGCAGAGTTATATGAAGAGCTAGATGAAGATTTTTTGGCCTTGGAGATTCTGCAAAAGCTTGACACTCGGTGCCTGGCTAATCTTGTAGTCAGTAGGAACGAGAATGCCGAGGATGATGGCTACTACAGTGAACATTTTGTGACCCAACATGATCTTCTTAGAGAGCTGGCTATCTACCAAACGAAACAAGACCCAAATAGAAAAAGACTGTTTATTGGAAGCGCCAGAGACAATCTGCCCAAGTCCTTGACAGAACAGAAGCATCGACCTATCAAGCCTCGCCTAGTATCTATCTCTTCTGATGGGGCGTTCTCAACAATTTGGCATAACATTGAACTAGCTGAGGTTGAGGTTCTAGTTTTGAATTTCAACACAGAGAACTATGCTTTGCCCGAATTTATGGAGAAAATGGAGAACCTGAAGGTTCTAATAGTCAGAAATCATGGTTCCTTGCCTGCTGAATTGAGTAATTTTCACCTACTGGATTCTTTACCAAATCTGAAGAGAATCAGACTAGAGAACGTTTCAATTCCTTCCATAACCAAGCATCCCATACAGTTGAAGAGTCTAAAGAAGATCTCTTTATTCATGTGTAGCATTGGTCAAGCTTTCAGCAATTGTTCCTTCCAAATTTCAGATGCATTTCCATATTTAGAGGAAATGAATATTGACTACAGCAATGATTTGTTGGAATTGCCTGACGAGCTCTGCAATCTGATTTGGCTGAAAAAGCTCAGTATCACAAACTGTCATAAGCTATCCGTCTTGCCTTATGAGATTGGAGGGCTGATTAACTTAGAAGTTTTGAGGCTGAGGGCTTGTACAAACTTGGAAATACTTCCAAGATCTATCAAACACCTGAAGAAGCTAAATGTTCTTGACATAGCTAACTGTGTCAGCATTGTGGAGTTGTCTGAAGACATTGGCGAACTAAGCAGTTTAAGAAAGATCAACATGAGACAGTGCTCTAGATTGCAAGAGCTACCACTATCAGTTTGGGATCTTAAGCAACTAGAGGAAGTGATATGTGATGAGGGCGTAGGAGGGTTGTGGGAACCCTTCCTACCCAGACTCCGCAACGTAAGGGTGGTGATCATGAAAGAAGAATTTAACCTAGATTGGCTCCAAAATCCCACGGTACCTGTACGTCCAGCTGTTCCTGAACGCCCGACTGTTCCTGAACGCCCACCAGTTCTTGAAGGTCCACCAGTTACTGTTGGGTTGGATTTGCCTTTGAAGGATTTGAAGAAATTTTTTCTCAAGGAAGGTAAGTCAAGGCTCGTGCTTATTGCTAATGGAGGACTCGGGAAAACTACGTTGGCGAGAAAGTTTTTTGAAGATCAGGACGTCAAAGGTATATTCAAGAAGAATATGTTCTTTGTTGTTGCTTCAAGTAAGCCCAGTTTGGAAGCTATTGTCCGAGAATTATATCGACAAAAGCAAGAACAAGCAGTACCGAAGAAGATTCGCGACTATAAAGCTGCAGTTCAATTGCTGGAAGACCTTCTAGAGGAAGCGGGAGAAGACCCTTTGCTGTTGGTACTGGATGATGTTTGGTCTCCCAAACTTCTTGATCTATTTAACAATTTCAAAATGCCAAATTACAAGTTTCTGGTGACTTCAAGATCTCAATTTGAAAATTTTGGTGAAACATATCCTTTAGAGCCTTTGAATCGTGAACATACAATGGAACTTTTTCGTGATTTAGCCCACTTGGGAGAGAAGATTTCTCTTCTAGAAGAAGTTCAAGAAAAGATTTGGAAGATATGCAAGGGCTATCCACTTATCATTAAAGTATTTGCAACATCAATTTGCAAACAGCCTGTGGAGAATTGGCAAAAATTTGTAATGAAAATGTCTGGAGCTTCTGTTTTGGATTCTGAAACTGAAGTGCTCATCTGCCTCCAAAGAAGCTTAGATCCTCTGGATAAAATCACCAGGCAATGTTTCATGGACCTTGGTTCATTCCCTGAAGACGAAAAAATCTCTGCTGCTGCCCTTATTGATATGTGGTCAGAGCTCTATCCTGAGCTAATTGAGGATACTTGCATTGAGAACCTCTACAAGCTGAACAGCCGGAGTCTGGTTGATCTTCAAGTCACAGGGAAGGAGAGGATACAACGGGATGGCTACTACACTGAACACTTTGTCATCCAGCATGACGTGCTTAGACAACTGGCGATCTTCCAAGCGAGACTAGAACCAATTGAACAGAGACAAAGACTTAATATTACAGACACATCTAGCAATCTACCCATTGGCCAAGAAAAAGAAGAAGAACCAAATTCAACTCTTCAGCCCATCAAGGCTCGCCTATTATCTATCTCTTCTGATGGTGTGTTCTCAACGAAATGGAATACCGTACAATTACCAGAAATTGAGGCACTCCTGATGAATATTAAGTCGAGTCGTAACTATGCGTTACCTCAATTTCTGGAGAGAACATCCAGATTAAAGGTTCTAATAATCACAAAGTATGGTCGTGATTCTGCTGAGTTGAGTAATTTTCAACTACTGGGTTTGTTACCAAATCTGAAGAGAATCAGATTAGAGACTGTTTTCACTCCTTCCATAACCAAGAACCTCACTCAACTGAAGAGTCTTGAGAAGATAACTTTGTCCTGGTGCGACGATATTGATAAAGCTTTTAGCAGCTCTTCCATCCAAATTCCACTTGCATTTCCGAAGCTAGTGGAATTAAACATCGACAATTGCAACGATTTAGTGAAATTGCCTGCCGAACTCTGTGATCTTGTTTCTCTAAAGAAACTCAGTGTTACCGAGTGTTATAATCTGGTTACCATACCAGAAGCAATTGGAAAGTTGGTCAAATTAGAATTGCTGAGGCTAAGAGGCTGTACGGAGTTGCAACAGCTTCCAGAATCGCTTCACAGCCTCCCAGAGTTGAACTTCCTTGACATAGCTGATTGCGTCAGCATGAAGGAGTTGCCTCAGAACATTGGAGCACTGAGCAGTTTAGAAAAGCTTGACATGAGAAAATGCTCTGGACTGGAAAAGCTTCCTTCATCAGTTATATATCTTCGTAAGTTACGGGAAGTGATATGCGATGAAGAGAAAAAAAAGTTATGGGGACGTTATTTGGGCTTTATGGAAAACCTAAGGAAGTTGGGTCCAGTGGAGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1483

Amino Acids

169.89

Weight (kDa)

5.64

Isoelectric Point (pI)

43.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 3 - 133 8.1e-28 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 172 - 304 3e-13 NB-ARC domain
LRR_14 PF23598 669 - 773 2.1e-09 Leucine-rich repeat region
NB-ARC PF00931 850 - 979 2.9e-11 NB-ARC domain
LRR_14 PF23598 1328 - 1414 8.4e-07 Leucine-rich repeat region
LRR_14 PF23598 1397 - 1478 1.1e-09 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000254)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25671 FvH4_1g29782 FvH4_2g06731 FvH4_2g09413 FvH4_2g12061 FvH4_2g12671 FvH4_2g14202 FvH4_3g27901 FvH4_3g30492 FvH4_3g36953 FvH4_4g10141 FvH4_4g11162 FvH4_5g27995 FvH4_5g34262 FvH4_6g10681 FvH4_6g13733 FvH4_6g18164 FvH4_6g25491 FvH4_6g25791 FvH4_6g27023 FvH4_6g34321 FvH4_7g05070
pyrus_communis pycom04g06850
rosa_chinensis RchiOBHm_Chr1g0322281 RchiOBHm_Chr1g0334981 RchiOBHm_Chr1g0371181 RchiOBHm_Chr2g0125461 RchiOBHm_Chr2g0127141 RchiOBHm_Chr2g0128961 RchiOBHm_Chr2g0132921 RchiOBHm_Chr3g0486471 RchiOBHm_Chr3g0492911 RchiOBHm_Chr4g0412131 RchiOBHm_Chr4g0426941 RchiOBHm_Chr4g0437331 RchiOBHm_Chr5g0018821 RchiOBHm_Chr5g0040021 RchiOBHm_Chr5g0051041 RchiOBHm_Chr6g0266871 RchiOBHm_Chr6g0271761 RchiOBHm_Chr7g0200931 RchiOBHm_Chr7g0220091
rosa_multiflora Rmu_co7991344.1_g000001 Rmu_sc0000172.1_g000008 Rmu_sc0000194.1_g000010 Rmu_sc0000446.1_g000026 Rmu_sc0000496.1_g000011 Rmu_sc0000530.1_g000013 Rmu_sc0000706.1_g000044 Rmu_sc0000745.1_g000029 Rmu_sc0001301.1_g000027 Rmu_sc0001520.1_g000008 Rmu_sc0001966.1_g000025 Rmu_sc0001989.1_g000007 Rmu_sc0002239.1_g000002 Rmu_sc0002816.1_g000004 Rmu_sc0003458.1_g000001 Rmu_sc0003825.1_g000031 Rmu_sc0003919.1_g000002 Rmu_sc0004053.1_g000007 Rmu_sc0004161.1_g000008 Rmu_sc0004368.1_g000033 Rmu_sc0004383.1_g000002 Rmu_sc0004923.1_g000002 Rmu_sc0005065.1_g000001 Rmu_sc0005478.1_g000001 Rmu_sc0006847.1_g000031 Rmu_sc0009556.1_g000002 Rmu_sc0010783.1_g000007 Rmu_sc0011424.1_g000022 Rmu_sc0013205.1_g000002 Rmu_sc0017407.1_g000001 Rmu_sc0023757.1_g000002 Rmu_sc0032346.1_g000001 Rmu_ssc0000008.1_g000014 Rmu_ssc0000158.1_g000033 Rmu_ssc0000217.1_g000028
rosa_roxburghii Rroxscaffold_1G00000480 Rroxscaffold_1G00026050 Rroxscaffold_1G00026250 Rroxscaffold_2G00085120 Rroxscaffold_2G00119710 Rroxscaffold_2G00126610 Rroxscaffold_2G00129340 Rroxscaffold_3G00245550 Rroxscaffold_5G00340210 Rroxscaffold_5G00340330 Rroxscaffold_6G00397180 Rroxscaffold_7G00216160
rosa_rugosa Rorug01G0040000 Rorug01G0067400 Rorug01G0092300 Rorug01G0197000 Rorug01G0474200 Rorug01G0482500 Rorug01G0489300 Rorug02G0142900 Rorug02G0259000 Rorug02G0283200 Rorug02G0341400 Rorug02G0341600 Rorug02G0370900 Rorug02G0379700 Rorug02G0386100 Rorug02G0387300 Rorug03G0112600 Rorug03G0265600 Rorug03G0298800 Rorug04G0019500 Rorug05G0069900 Rorug05G0070500 Rorug05G0256800 Rorug05G0287300 Rorug05G0592000 Rorug06G0057800 Rorug06G0082000 Rorug06G0118900 Rorug06G0176500 Rorug06G0423100 Rorug07G0050100 Rorug07G0095100 Rorug07G0202100 Rorug07G0216600 Rorug07G0244300 Rorug07G0245500
rosa_samantha Rh1DG154800 Rh2CG613000 Rh6BG016600 Rh6BG475100 Rh6CG013200 Rh7AG443800
rosa_wichuraiana Rw0G021840 Rw4G023650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4095
AatII GACGTC 1 cut(s) 2655
Acc65I GGTACC 2 cut(s) 725, 2443
AccB1I GGYRCC 4 cut(s) 725, 874, 1374, 2443
AccB7I CCANNNNNTGG 1 cut(s) 923
AccI GTMKAC 1 cut(s) 1245
AccII CGCG 2 cut(s) 1104, 2775
AciI CCGC 4 cut(s) 588, 1104, 2380, 2822
AclI AACGTT 1 cut(s) 1827
AclWI GGATC 3 cut(s) 513, 2313, 3218
AcoI YGGCCR 3 cut(s) 75, 830, 3565
AcyI GRCGYC 1 cut(s) 2652
AdeI CACNNNGTG 1 cut(s) 1374
AfiI CCNNNNNNNGG 4 cut(s) 768, 923, 2815, 3388
AflII CTTAAG 2 cut(s) 539, 2309
AjiI CACGTC 1 cut(s) 3469
AjnI CCWGG 7 cut(s) 577, 816, 916, 996, 1377, 3242, 3926
AjuI GAANNNNNNNTTGG 6 cut(s) 407, 439, 1443, 1475, 2606, 2638
Alw21I GWGCWC 9 cut(s) 251, 435, 570, 1013, 2013, 2273, 3201, 3327, 4273
Alw26I GTCTC 6 cut(s) 1527, 2257, 2868, 3493, 3514, 3851
AlwI GGATC 3 cut(s) 513, 2313, 3218
AlwNI CAGNNNCTG 3 cut(s) 331, 1286, 2519
Ama87I CYCGRG 1 cut(s) 2609
AoxI GGCC 4 cut(s) 75, 830, 1341, 3565
ApeKI GCWGC 7 cut(s) 772, 1037, 1286, 2786, 3296, 3299, 3957
Asp700I GAANNNNTTC 7 cut(s) 2142, 2678, 2808, 3000, 3048, 4191, 4323
Asp718I GGTACC 2 cut(s) 725, 2443
AspLEI GCGC 2 cut(s) 1195, 1529
AspS9I GGNCC 5 cut(s) 418, 814, 2507, 3259, 4436
AsuHPI GGTGA 8 cut(s) 460, 907, 1166, 1772, 2407, 2932, 2963, 3232
AsuII TTCGAA 1 cut(s) 744
AvaI CYCGRG 1 cut(s) 2609
AvaII GGWCC 5 cut(s) 418, 814, 2507, 3259, 4436
AxyI CCTNAGG 1 cut(s) 4424
BaeI ACNNNNGTAYC 4 cut(s) 717, 717, 750, 750
BalI TGGCCA 2 cut(s) 77, 3567
BanI GGYRCC 4 cut(s) 725, 874, 1374, 2443
BanII GRGCYC 4 cut(s) 435, 1013, 2013, 3327
BarI GAAGNNNNNNTAC 2 cut(s) 465, 497
BauI CACGAG 1 cut(s) 2585
BbsI GAAGAC 6 cut(s) 753, 1272, 2229, 2811, 2835, 3285
Bbv12I GWGCWC 9 cut(s) 251, 435, 570, 1013, 2013, 2273, 3201, 3327, 4273
BbvCI CCTCAGC 3 cut(s) 1647, 2117, 4148
BbvI GCAGC 7 cut(s) 759, 1024, 1273, 2773, 3283, 3286, 3969
BccI CCATC 8 cut(s) 67, 1017, 1415, 1601, 3425, 3614, 3632, 3974
BcgI CGANNNNNNTGC 2 cut(s) 740, 774
BciT130I CCWGG 7 cut(s) 579, 818, 918, 998, 1379, 3244, 3928
BciVI GTATCC 2 cut(s) 3340, 3414
BclI TGATCA 1 cut(s) 2397
BcoDI GTCTC 6 cut(s) 1527, 2257, 2868, 3493, 3514, 3851
BfmI CTRYAG 5 cut(s) 27, 710, 1429, 1978, 2787
BfoI RGCGCY 1 cut(s) 1530
BfrI CTTAAG 2 cut(s) 539, 2309
BfuI GTATCC 2 cut(s) 3340, 3414
BglII AGATCT 4 cut(s) 842, 1881, 2150, 2930
BisI GCNGC 7 cut(s) 773, 1038, 1287, 2787, 3297, 3300, 3958
BlsI GCNGC 7 cut(s) 774, 1039, 1288, 2788, 3298, 3301, 3959
Bme1390I CCNGG 7 cut(s) 579, 818, 918, 998, 1379, 3244, 3928
Bme18I GGWCC 5 cut(s) 418, 814, 2507, 3259, 4436
BmeT110I CYCGRG 1 cut(s) 2609
BmgBI CACGTC 1 cut(s) 3469
BmgT120I GGNCC 5 cut(s) 418, 814, 2507, 3259, 4436
BmrFI CCNGG 7 cut(s) 579, 818, 918, 998, 1379, 3244, 3928
BmrI ACTGGG 3 cut(s) 1274, 2700, 3830
BmsI GCATC 6 cut(s) 237, 575, 955, 1576, 1861, 1933
BmuI ACTGGG 3 cut(s) 1274, 2700, 3830
BpiI GAAGAC 6 cut(s) 753, 1272, 2229, 2811, 2835, 3285
BpmI CTGGAG 3 cut(s) 561, 3186, 3761
Bpu10I CCTNAGC 5 cut(s) 477, 1647, 2117, 3333, 4148
Bpu14I TTCGAA 1 cut(s) 744
BpuEI CTTGAG 3 cut(s) 749, 2549, 3929
BsaHI GRCGYC 1 cut(s) 2652
BsaI GGTCTC 1 cut(s) 2868
BsaJI CCNNGG 8 cut(s) 423, 996, 997, 1249, 1344, 1413, 2439, 3262
BsaXI ACNNNNNCTCC 4 cut(s) 2361, 2391, 3159, 3189
Bsc4I CCNNNNNNNGG 4 cut(s) 768, 923, 2815, 3388
Bse21I CCTNAGG 1 cut(s) 4424
Bse3DI GCAATG 3 cut(s) 975, 1990, 3254
BseBI CCWGG 7 cut(s) 579, 818, 918, 998, 1379, 3244, 3928
BseDI CCNNGG 8 cut(s) 423, 996, 997, 1249, 1344, 1413, 2439, 3262
BseLI CCNNNNNNNGG 4 cut(s) 768, 923, 2815, 3388
BseMI GCAATG 3 cut(s) 975, 1990, 3254
BseRI GAGGAG 1 cut(s) 164
BseXI GCAGC 7 cut(s) 759, 1024, 1273, 2773, 3283, 3286, 3969
BsgI GTGCAG 1 cut(s) 1155
Bsh1236I CGCG 2 cut(s) 1104, 2775
BshFI GGCC 4 cut(s) 77, 832, 1343, 3567
BshNI GGYRCC 4 cut(s) 725, 874, 1374, 2443
BsiHKAI GWGCWC 9 cut(s) 251, 435, 570, 1013, 2013, 2273, 3201, 3327, 4273
BsiHKCI CYCGRG 1 cut(s) 2609
BsiSI CCGG 2 cut(s) 833, 3382
BslFI GGGAC 1 cut(s) 4410
BslI CCNNNNNNNGG 4 cut(s) 768, 923, 2815, 3388
BsmAI GTCTC 6 cut(s) 1527, 2257, 2868, 3493, 3514, 3851
BsmFI GGGAC 1 cut(s) 4410
BsmI GAATGC 1 cut(s) 1414
BsnI GGCC 4 cut(s) 77, 832, 1343, 3567
Bso31I GGTCTC 1 cut(s) 2868
BsoBI CYCGRG 1 cut(s) 2609
Bsp119I TTCGAA 1 cut(s) 744
Bsp1286I GDGCHC 9 cut(s) 251, 435, 570, 1013, 2013, 2273, 3201, 3327, 4273
Bsp1407I TGTACA 2 cut(s) 700, 2126
Bsp68I TCGCGA 1 cut(s) 2775
BspACI CCGC 4 cut(s) 588, 1104, 2380, 2822
BspANI GGCC 4 cut(s) 77, 832, 1343, 3567
BspFNI CGCG 2 cut(s) 1104, 2775
BspHI TCATGA 1 cut(s) 2400
BspMAI CTGCAG 1 cut(s) 2791
BspPI GGATC 3 cut(s) 513, 2313, 3218
BspQI GCTCTTC 2 cut(s) 1314, 3967
BspT104I TTCGAA 1 cut(s) 744
BspT107I GGYRCC 4 cut(s) 725, 874, 1374, 2443
BspTI CTTAAG 2 cut(s) 539, 2309
BspTNI GGTCTC 1 cut(s) 2868
BsrDI GCAATG 3 cut(s) 975, 1990, 3254
BsrGI TGTACA 2 cut(s) 700, 2126
BssECI CCNNGG 8 cut(s) 423, 996, 997, 1249, 1344, 1413, 2439, 3262
BssNI GRCGYC 1 cut(s) 2652
BssSI CACGAG 1 cut(s) 2585
BssT1I CCWWGG 4 cut(s) 423, 1249, 1344, 3262
Bst2BI CACGAG 1 cut(s) 2585
Bst2UI CCWGG 7 cut(s) 579, 818, 918, 998, 1379, 3244, 3928
BstACI GRCGYC 1 cut(s) 2652
BstAFI CTTAAG 2 cut(s) 539, 2309
BstAPI GCANNNNNTGC 2 cut(s) 23, 3469
BstAUI TGTACA 2 cut(s) 700, 2126
BstBI TTCGAA 1 cut(s) 744
BstC8I GCNNGC 4 cut(s) 1473, 1761, 3616, 4041
BstDSI CCRYGG 1 cut(s) 2439
BstEII GGTNACC 1 cut(s) 4102
BstENI CCTNNNNNAGG 1 cut(s) 2813
BstFNI CGCG 2 cut(s) 1104, 2775
BstH2I RGCGCY 1 cut(s) 1530
BstHHI GCGC 2 cut(s) 1195, 1529
BstMAI GTCTC 6 cut(s) 1527, 2257, 2868, 3493, 3514, 3851
BstMWI GCNNNNNNNGC 7 cut(s) 23, 109, 1192, 2008, 2035, 2238, 3469
BstNI CCWGG 7 cut(s) 579, 818, 918, 998, 1379, 3244, 3928
BstPI GGTNACC 1 cut(s) 4102
BstSCI CCNGG 7 cut(s) 577, 816, 916, 996, 1377, 3242, 3926
BstSFI CTRYAG 5 cut(s) 27, 710, 1429, 1978, 2787
BstUI CGCG 2 cut(s) 1104, 2775
BstV1I GCAGC 7 cut(s) 759, 1024, 1273, 2773, 3283, 3286, 3969
BstV2I GAAGAC 6 cut(s) 753, 1272, 2229, 2811, 2835, 3285
BstX2I RGATCY 7 cut(s) 505, 842, 1881, 2150, 2305, 2930, 3223
BstXI CCANNNNNNTGG 1 cut(s) 1084
BstYI RGATCY 7 cut(s) 505, 842, 1881, 2150, 2305, 2930, 3223
Bsu36I CCTNAGG 1 cut(s) 4424
BsuI GTATCC 2 cut(s) 3340, 3414
BsuRI GGCC 4 cut(s) 77, 832, 1343, 3567
BtgI CCRYGG 1 cut(s) 2439
BtgZI GCGATG 2 cut(s) 86, 4386
BtrI CACGTC 1 cut(s) 3469
BtsI GCAGTG 1 cut(s) 570
BtuMI TCGCGA 1 cut(s) 2775
Cac8I GCNNGC 4 cut(s) 1473, 1761, 3616, 4041
CaiI CAGNNNCTG 3 cut(s) 331, 1286, 2519
CciI TCATGA 1 cut(s) 2400
CfoI GCGC 2 cut(s) 1195, 1529
Cfr13I GGNCC 5 cut(s) 418, 814, 2507, 3259, 4436
CseI GACGC 1 cut(s) 4225
DraI TTTAAA 1 cut(s) 94
DraIII CACNNNGTG 1 cut(s) 1374
EaeI YGGCCR 3 cut(s) 75, 830, 3565
Ecl136II GAGCTC 4 cut(s) 433, 1011, 2011, 3325
Eco130I CCWWGG 4 cut(s) 423, 1249, 1344, 3262
Eco24I GRGCYC 4 cut(s) 435, 1013, 2013, 3327
Eco31I GGTCTC 1 cut(s) 2868
Eco32I GATATC 1 cut(s) 1067
Eco47I GGWCC 5 cut(s) 418, 814, 2507, 3259, 4436
Eco53kI GAGCTC 4 cut(s) 433, 1011, 2011, 3325
Eco81I CCTNAGG 1 cut(s) 4424
Eco88I CYCGRG 1 cut(s) 2609
Eco91I GGTNACC 1 cut(s) 4102
EcoICRI GAGCTC 4 cut(s) 433, 1011, 2011, 3325
EcoNI CCTNNNNNAGG 1 cut(s) 2813
EcoO109I RGGNCCY 1 cut(s) 418
EcoO65I GGTNACC 1 cut(s) 4102
EcoRI GAATTC 1 cut(s) 1118
EcoRII CCWGG 7 cut(s) 577, 816, 916, 996, 1377, 3242, 3926
EcoRV GATATC 1 cut(s) 1067
EcoT14I CCWWGG 4 cut(s) 423, 1249, 1344, 3262
EcoT22I ATGCAT 1 cut(s) 1948
EcoT38I GRGCYC 4 cut(s) 435, 1013, 2013, 3327
ErhI CCWWGG 4 cut(s) 423, 1249, 1344, 3262
FalI AAGNNNNNCTT 4 cut(s) 407, 439, 1173, 1205
FaqI GGGAC 1 cut(s) 4410
FauI CCCGC 1 cut(s) 2815
FbaI TGATCA 1 cut(s) 2397
FblI GTMKAC 1 cut(s) 1245
Fnu4HI GCNGC 7 cut(s) 773, 1038, 1287, 2787, 3297, 3300, 3958
FriOI GRGCYC 4 cut(s) 435, 1013, 2013, 3327
Fsp4HI GCNGC 7 cut(s) 773, 1038, 1287, 2787, 3297, 3300, 3958
GlaI GCGC 2 cut(s) 1194, 1528
GluI GCNGC 7 cut(s) 773, 1038, 1287, 2787, 3297, 3300, 3958
GsuI CTGGAG 3 cut(s) 561, 3186, 3761
HaeII RGCGCY 1 cut(s) 1530
HaeIII GGCC 4 cut(s) 77, 832, 1343, 3567
HapII CCGG 2 cut(s) 833, 3382
HgaI GACGC 1 cut(s) 4225
HhaI GCGC 2 cut(s) 1195, 1529
Hin1I GRCGYC 1 cut(s) 2652
Hin6I GCGC 2 cut(s) 1193, 1527
HinP1I GCGC 2 cut(s) 1193, 1527
HindIII AAGCTT 7 cut(s) 533, 1361, 1911, 3217, 3948, 4289, 4322
HpaII CCGG 2 cut(s) 833, 3382
HphI GGTGA 8 cut(s) 460, 907, 1166, 1772, 2407, 2932, 2963, 3232
Hpy166II GTNNAC 4 cut(s) 1246, 1436, 2510, 2984
Hpy8I GTNNAC 4 cut(s) 1246, 1436, 2510, 2984
Hpy99I CGWCG 2 cut(s) 186, 3938
HpyCH4IV ACGT 8 cut(s) 825, 1827, 2385, 2452, 2621, 2652, 3468, 4399
HpyF10VI GCNNNNNNNGC 7 cut(s) 23, 109, 1192, 2008, 2035, 2238, 3469
HpySE526I ACGT 8 cut(s) 825, 1827, 2385, 2452, 2621, 2652, 3468, 4399
Hsp92I GRCGYC 1 cut(s) 2652
HspAI GCGC 2 cut(s) 1193, 1527
KpnI GGTACC 2 cut(s) 729, 2447
Ksp22I TGATCA 1 cut(s) 2397
LguI GCTCTTC 2 cut(s) 1314, 3967
LmnI GCTCC 4 cut(s) 580, 2433, 3167, 4268
Lsp1109I GCAGC 7 cut(s) 759, 1024, 1273, 2773, 3283, 3286, 3969
LweI GCATC 6 cut(s) 237, 575, 955, 1576, 1861, 1933
MaeII ACGT 8 cut(s) 825, 1827, 2385, 2452, 2621, 2652, 3468, 4399
MfeI CAATTG 5 cut(s) 1921, 2795, 3510, 4015, 4119
MflI RGATCY 7 cut(s) 505, 842, 1881, 2150, 2305, 2930, 3223
MhlI GDGCHC 9 cut(s) 251, 435, 570, 1013, 2013, 2273, 3201, 3327, 4273
MlsI TGGCCA 2 cut(s) 77, 3567
MluNI TGGCCA 2 cut(s) 77, 3567
MlyI GAGTC 7 cut(s) 967, 1879, 2370, 2601, 3394, 3721, 3913
MmeI TCCRAC 4 cut(s) 478, 1069, 1974, 2508
Mox20I TGGCCA 2 cut(s) 77, 3567
Mph1103I ATGCAT 1 cut(s) 1948
MroXI GAANNNNTTC 7 cut(s) 2142, 2678, 2808, 3000, 3048, 4191, 4323
MscI TGGCCA 2 cut(s) 77, 3567
MslI CAYNNNNRTG 2 cut(s) 1082, 1298
Msp20I TGGCCA 2 cut(s) 77, 3567
MspA1I CMGCKG 1 cut(s) 2459
MspCI CTTAAG 2 cut(s) 539, 2309
MspI CCGG 2 cut(s) 833, 3382
MspR9I CCNGG 7 cut(s) 579, 818, 918, 998, 1379, 3244, 3928
MssI GTTTAAAC 1 cut(s) 94
MunI CAATTG 5 cut(s) 1921, 2795, 3510, 4015, 4119
Mva1269I GAATGC 1 cut(s) 1414
MvaI CCWGG 7 cut(s) 579, 818, 918, 998, 1379, 3244, 3928
MvnI CGCG 2 cut(s) 1104, 2775
MwoI GCNNNNNNNGC 7 cut(s) 23, 109, 1192, 2008, 2035, 2238, 3469
NmeAIII GCCGAG 1 cut(s) 1438
NmuCI GTSAC 5 cut(s) 264, 895, 1444, 2920, 3403
NruI TCGCGA 1 cut(s) 2775
NsiI ATGCAT 1 cut(s) 1948
NspV TTCGAA 1 cut(s) 744
PagI TCATGA 1 cut(s) 2400
PasI CCCWGGG 1 cut(s) 997
PciSI GCTCTTC 2 cut(s) 1314, 3967
PcsI WCGNNNNNNNCGW 1 cut(s) 1410
PctI GAATGC 1 cut(s) 1414
PdmI GAANNNNTTC 7 cut(s) 2142, 2678, 2808, 3000, 3048, 4191, 4323
PflMI CCANNNNNTGG 1 cut(s) 923
PfoI TCCNGGA 3 cut(s) 577, 816, 916
PkrI GCNGC 7 cut(s) 774, 1039, 1288, 2788, 3298, 3301, 3959
PleI GAGTC 7 cut(s) 967, 1878, 2370, 2601, 3393, 3720, 3912
PmeI GTTTAAAC 1 cut(s) 94
PpsI GAGTC 7 cut(s) 967, 1878, 2370, 2601, 3393, 3720, 3912
PpuMI RGGWCCY 1 cut(s) 418
PsiI TTATAA 1 cut(s) 4095
Psp124BI GAGCTC 4 cut(s) 435, 1013, 2013, 3327
Psp1406I AACGTT 1 cut(s) 1827
Psp5II RGGWCCY 1 cut(s) 418
Psp6I CCWGG 7 cut(s) 577, 816, 916, 996, 1377, 3242, 3926
PspEI GGTNACC 1 cut(s) 4102
PspGI CCWGG 7 cut(s) 577, 816, 916, 996, 1377, 3242, 3926
PspPI GGNCC 5 cut(s) 418, 814, 2507, 3259, 4436
PspPPI RGGWCCY 1 cut(s) 418
PsrI GAACNNNNNNTAC 4 cut(s) 2352, 2384, 2742, 2774
PstI CTGCAG 1 cut(s) 2791
PstNI CAGNNNCTG 3 cut(s) 331, 1286, 2519
PsuI RGATCY 7 cut(s) 505, 842, 1881, 2150, 2305, 2930, 3223
PvuII CAGCTG 1 cut(s) 2459
RruI TCGCGA 1 cut(s) 2775
RseI CAYNNNNRTG 2 cut(s) 1082, 1298
SacI GAGCTC 4 cut(s) 435, 1013, 2013, 3327
SapI GCTCTTC 2 cut(s) 1314, 3967
SatI GCNGC 7 cut(s) 773, 1038, 1287, 2787, 3297, 3300, 3958
Sau96I GGNCC 5 cut(s) 418, 814, 2507, 3259, 4436
SchI GAGTC 7 cut(s) 967, 1879, 2370, 2601, 3394, 3721, 3913
ScrFI CCNGG 7 cut(s) 579, 818, 918, 998, 1379, 3244, 3928
SduI GDGCHC 9 cut(s) 251, 435, 570, 1013, 2013, 2273, 3201, 3327, 4273
SfaNI GCATC 6 cut(s) 237, 575, 955, 1576, 1861, 1933
SfcI CTRYAG 5 cut(s) 27, 710, 1429, 1978, 2787
SfuI TTCGAA 1 cut(s) 744
SinI GGWCC 5 cut(s) 418, 814, 2507, 3259, 4436
SmiMI CAYNNNNRTG 2 cut(s) 1082, 1298
SmlI CTYRAG 5 cut(s) 539, 764, 2309, 2564, 3908
SmoI CTYRAG 5 cut(s) 539, 764, 2309, 2564, 3908
SsiI CCGC 4 cut(s) 588, 1104, 2380, 2822
SspI AATATT 3 cut(s) 1972, 3535, 3703
SstI GAGCTC 4 cut(s) 435, 1013, 2013, 3327
StyD4I CCNGG 7 cut(s) 577, 816, 916, 996, 1377, 3242, 3926
StyI CCWWGG 4 cut(s) 423, 1249, 1344, 3262
TaiI ACGT 8 cut(s) 828, 1830, 2388, 2455, 2624, 2655, 3471, 4402
TaqII GACCGA 1 cut(s) 252
TatI WGTACW 3 cut(s) 700, 805, 2126
TseFI GTSAC 5 cut(s) 264, 895, 1444, 2920, 3403
TseI GCWGC 7 cut(s) 772, 1037, 1286, 2786, 3296, 3299, 3957
Tsp45I GTSAC 5 cut(s) 264, 895, 1444, 2920, 3403
TspGWI ACGGA 2 cut(s) 2056, 4181
Van91I CCANNNNNTGG 1 cut(s) 923
Vha464I CTTAAG 2 cut(s) 539, 2309
VpaK11BI GGWCC 5 cut(s) 418, 814, 2507, 3259, 4436
XagI CCTNNNNNAGG 1 cut(s) 2813
XbaI TCTAGA 3 cut(s) 2273, 2812, 3040
XcmI CCANNNNNNNNNTGG 1 cut(s) 2298
XmiI GTMKAC 1 cut(s) 1245
XmnI GAANNNNTTC 7 cut(s) 2142, 2678, 2808, 3000, 3048, 4191, 4323
ZraI GACGTC 1 cut(s) 2653
Zsp2I ATGCAT 1 cut(s) 1948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.