Rorug01G0482500

Protein of unknown function (DUF4050)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000254
Physical Location & Seq
Forward (+)
56996099 .. 57005027
8929 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0482500.1

Sequence Viewer

Length: 3996 bp
ATGGGTGTCGAGAATTATCATGTCATTGAGCTTGTAGGCGAAGGTTCCTTTGGGAAGGTATACAAGGGAAGACGCAAGTTCACTGGGCAGACCGTTGCAATGAAATTTATTATGAAACATGGGAAAAGCGACAAGGATATCCACAATTTAAGACAAGAAATAGAGATTCTAAGGAAGCTAAAACATGAAAATATCATTGAAATGCTTGATTCCTTTGAAAGCCCACAAGAATTCTGTGTTGTCACAGAGTTTGCACAAGGTGAACTATTTGAAATTCTTGAAGATGATAAGCACCTTCCAGAAGAACAAGTTCAAGCAATTGCTAAGCAATTGGTGAGAGCATTGCATTATCTCCATTCAAACCGCATCATCCATCGTGACATGAAACCACAAAACATTCTCATTGGTGCTGGTTCTATTGTTAAGCTCTGTGACTTTGGGTTTGCACGTGCAATGTCCACAAATACTGTTGTTTTGCGATCCATAAAAGGTACTCCCCTGTACATGGCTCCTGAACTAGTACGGGAACAACCTTACAACCACACTGCAGATTTATGGTCTCTCGGAGTTATATTATATGAATTATTTGTTGGCCAGCCTCCATTTTATACAAATTCAGTATATGCACTCATTCGGCATATTGTGAAGGATCCAGTTAAATACCCTGACAACATGAGTTCAAATTTTAAAAACTTTCTGAAGGGATTGCTCAATAAGATGCCTCAAAATCGTTTGACTTGGCCAGCTCTTTTGGAACACCCATTTGTTAAGGAGACAACTGAAGAACTGGAGGCTAGGGAAATGCGTTCTGCTACTGCTGCAGAAAGGGGGTGCGTTGCAGCATGGAGGGGTGAAGGAAACAAAATCCAAACATCAGGTGGCTTGGCTGTTTCTAGTCCTGCTTCTTCAGAGAACAACAGTGGAATAAGTTTCCAAAATGATGCTCAAACAAATATTCCAGACTCTAAAGCAGTCAATTCTTCTCCAAATGAGGAGTTTCCAGGGTTTGCAAATCCTGATAAAGTTAAACAGTCAGGTTGTCAAATTCTCGACAGACTGGAAAATAACTCTCGTACAGTTAAAGGTGCACAAATAATTGGTCAAGATAATGAAGCATTAGCACATGTTTTGCTACCACTAAAAAGATGCTCGCAAGGATCCCAAAACTCTAGCAGGGATCAAGATATTCTTACTTCAAACCAGTCTTTAAGAATCCTTTCAAACTTAGTTGCAGCTGGTGCCATCACTTCCAGTGGCCTACTTGATGAAATCATACATGAAATTCTTGTTTATACCACCTTCATTGTGGGCATCAAATCTTCTGAACTGAATGAGTTTAGAGCAAAGAGTTTCTCAGTAATTAAAGTTTTGGTAGACATTGCTGGACATGGCATTGGAGGCTCATATTTCAGGCACTGGGTTGCTTTAGCTGATATTTTTTCACAGGTTGTTGGTGGCAGTGAAGATGCATCTGGAAGAGTTTTGTTTGAGTCCATAGCTTGCATTACTGTCATGTTAACAAGAGTTAATCAGGGTCTGAAACAGGCTGTTTCTGATCCTAATGAAGCAGTGAAACAGATTTTGGATCATGCTAAAACATCTGGTTTAGTGGATCAGCTTTGTCTCTGCTTAGCTACTGCTGGAGCAAGTCTTATTTCGGGTCCTTCAAATATGTCACGTTCAGCCTGTGAAGCATGCATGGCTATTTGGTTGCTGGTAGATACATCAGAGTTTTTTTCGACAAAAGGAAATGCCATTTCCTTTCCGCTAAACACTTTGCGCAGTCCATCAAGGGCTCAAGATCGAGGAGGTTCTTTGATTGGAACAGAATCAGCAAGACTAGTAGCTGTAGTTACTAGAGCATTAGTTAGATCTAAACCAGTTCAGGTTGCCATCCATTATTGCCTTCATCAACGTCTTGAGGCTTCACTCTGTGCTGGTATTCAGCTATTGTTGAGGTGCTGCTTGCAGAGTGGAATTGTTCCTGGCATTCTTTGTGGCCTGCCTAGTTCTTTACCTGTAACTACTGTTGTCAGTGGTGGAGGTGATGGAACCATAATTTTTGAGATATTCTCTTTGTTGTCTGTGTGCATTTCATCTCAAAATAAAGATCCACAAGCAATTGAGACAACCAACTTGAAGAGCAAAATAACCGATCCCACTATGTTGGTTCTGCATTCCTGCCTCATCCTTGCATCAGTTGCACAATGTTTAAAGGCAACTGGAAGAAATTCTGCATTGTTTATGCTTACTACATCCTCGAAGAAGCAGCTTTCTCGACTTTCTGCCCTTGCTCATCACTTTTCTTCCGGTGAGAGCACAAATACTTCCTCTCAAACTCACAGTGCAGCAGCCATGTTGGCACTGGCATCTATTTTGTCCCTTGAATCTGGAGCTTCTGTTGGGTCCTCTGTCTTTGAGGTAGCAGTGCCTTTGATTCCTCAAACTGCGACACTTTGTGAATGCCTCAAACTTTCATCAAACAATGAGATTGTATCGGGTCCTAATGGCACCAACGGTGTTCTCTCAAACTGGCATGGTCTTAGGGATGGATGTGTTGGCTTGCTGGAGGCCAGACTGAGATGGGGAGGACCCTTAGCTGTTCAACAACTTTGTGCAAGCAATATCCCTCTGCTTCTAATCAATTTGTTAGCCAAAAACCAACAAAATGCTTCTCCTCAAGAAGTTGCCAGCACAAATGATCAAGTTGGGCTTTCCCCTATTGGAGTTGTATGGACAGTATCATCTATTTGTCAGTGCCTTTCTGGTGGAGCCTTAACCTTTCGTCAGATTCTGCTTAGAAGTGAGCATATCAAACTCCTCTCTGACTTGATATCTGACATGCATCTCAAACTTGTAAAGTCCTGGGTTGGGCCTGGTGGAGGAATGGATGGCGTAAGAGATATAACAAATGCAGTAATTGACCTCTTGGCATTTCCTTTTGTGGCTGTTCAGAATGCTCCAGGATTGCCATCAGCGACTGCCTCAGTGAACAGTGGGATCCTTCTGAACATGGGTTCACCAGGTGTGAAAGTTGGCGTTGAAGACAGAGACATGGTGAAGGTGATTGAGGAAGACCTGGGAAAATATATTAAAATCCTTTTGGAGGTTGGAGTGCCTGGTATCATTCTTTGGTGTCTAGAACATTTGGAGTTAAAAGATTTGGGAAGACCTGTGGCCTTTCTGGCTAAAATGATTGGTCACCGACCATTGGCAGTTCAACTTGTGGGTAAAGGCTTGTTGGATCCTACTAGGATGAGAAGATTGCTCGATCGTTCAAGCCCAAAAGAAGTCATGCTGGATGTTCTGATGATTGTTTCTGATTTGGCACGCATGGACAAGGGATTCTACGAATACATTAATCGAGCTTCTCTGCTGGAGTTCTTCAAGGGATTTCTTACCCATGAAGATCCTAGTGTGCGTTCGAAGGCTTGCAGTGCTCTAGGCAATATGTGCCGGCACAGCTCCTACTTTTACAGTTCCCTAGCAAAGAATCAAATCATTGGTCTCCTTATTGATCGATGCTCTGACCCAGACAAACGTACACGGAAATTTGCTTGTTTTGCTATTGGCAATGCCGCTTACCATAATGATATGTTGTATGAAGAGCTGCGGAGATCTATCCCCCAGCTTGCCAATTTGTTGCTTTCATCTGAGGAAGACAAGACTAAAGCAAATGCAGCAGGTGCACTAAGCAATCTTATCCGCAACTCCAACAAACTTTGCGAAGACATTGTCTCTAGAGGAGCCATGCAGTCTTTGCTGAAGTTGGTGGCAGAGTGCTCAGTGTTGGCTCTGAACCCAAGTAGGAAAGATTCAGCGCAAGAGTCACCTCTCAAAATAGCTCTGTTTTCATTGGCAAAAATGTGCTCGCATCCGCCCTGCAGAGATTTCCTCCGTTCATCTGAGTTGTTCCCCGTGATCGGACGGCTCCGCCAATCCCCGGAATCAACAATTGCCAACTACGCCTCCGCCATCATCAACAAAGTTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4050

Protein Analysis

1331

Amino Acids

144.62

Weight (kDa)

8.23

Isoelectric Point (pI)

49.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 6 - 256 1.1e-78 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 8 - 251 3.4e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000254)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25671 FvH4_1g29782 FvH4_2g06731 FvH4_2g09413 FvH4_2g12061 FvH4_2g12671 FvH4_2g14202 FvH4_3g27901 FvH4_3g30492 FvH4_3g36953 FvH4_4g10141 FvH4_4g11162 FvH4_5g27995 FvH4_5g34262 FvH4_6g10681 FvH4_6g13733 FvH4_6g18164 FvH4_6g25491 FvH4_6g25791 FvH4_6g27023 FvH4_6g34321 FvH4_7g05070
pyrus_communis pycom04g06850
rosa_chinensis RchiOBHm_Chr1g0322281 RchiOBHm_Chr1g0334981 RchiOBHm_Chr1g0371181 RchiOBHm_Chr2g0125461 RchiOBHm_Chr2g0127141 RchiOBHm_Chr2g0128961 RchiOBHm_Chr2g0132921 RchiOBHm_Chr3g0486471 RchiOBHm_Chr3g0492911 RchiOBHm_Chr4g0412131 RchiOBHm_Chr4g0426941 RchiOBHm_Chr4g0437331 RchiOBHm_Chr5g0018821 RchiOBHm_Chr5g0040021 RchiOBHm_Chr5g0051041 RchiOBHm_Chr6g0266871 RchiOBHm_Chr6g0271761 RchiOBHm_Chr7g0200931 RchiOBHm_Chr7g0220091
rosa_multiflora Rmu_co7991344.1_g000001 Rmu_sc0000172.1_g000008 Rmu_sc0000194.1_g000010 Rmu_sc0000446.1_g000026 Rmu_sc0000496.1_g000011 Rmu_sc0000530.1_g000013 Rmu_sc0000706.1_g000044 Rmu_sc0000745.1_g000029 Rmu_sc0001301.1_g000027 Rmu_sc0001520.1_g000008 Rmu_sc0001966.1_g000025 Rmu_sc0001989.1_g000007 Rmu_sc0002239.1_g000002 Rmu_sc0002816.1_g000004 Rmu_sc0003458.1_g000001 Rmu_sc0003825.1_g000031 Rmu_sc0003919.1_g000002 Rmu_sc0004053.1_g000007 Rmu_sc0004161.1_g000008 Rmu_sc0004368.1_g000033 Rmu_sc0004383.1_g000002 Rmu_sc0004923.1_g000002 Rmu_sc0005065.1_g000001 Rmu_sc0005478.1_g000001 Rmu_sc0006847.1_g000031 Rmu_sc0009556.1_g000002 Rmu_sc0010783.1_g000007 Rmu_sc0011424.1_g000022 Rmu_sc0013205.1_g000002 Rmu_sc0017407.1_g000001 Rmu_sc0023757.1_g000002 Rmu_sc0032346.1_g000001 Rmu_ssc0000008.1_g000014 Rmu_ssc0000158.1_g000033 Rmu_ssc0000217.1_g000028
rosa_roxburghii Rroxscaffold_1G00000480 Rroxscaffold_1G00026050 Rroxscaffold_1G00026250 Rroxscaffold_2G00085120 Rroxscaffold_2G00119710 Rroxscaffold_2G00126610 Rroxscaffold_2G00129340 Rroxscaffold_3G00245550 Rroxscaffold_5G00340210 Rroxscaffold_5G00340330 Rroxscaffold_6G00397180 Rroxscaffold_7G00216160
rosa_rugosa Rorug01G0040000 Rorug01G0067400 Rorug01G0092300 Rorug01G0197000 Rorug01G0474200 Rorug01G0482500 Rorug01G0489300 Rorug02G0142900 Rorug02G0259000 Rorug02G0283200 Rorug02G0341400 Rorug02G0341600 Rorug02G0370900 Rorug02G0379700 Rorug02G0386100 Rorug02G0387300 Rorug03G0112600 Rorug03G0265600 Rorug03G0298800 Rorug04G0019500 Rorug05G0069900 Rorug05G0070500 Rorug05G0256800 Rorug05G0287300 Rorug05G0592000 Rorug06G0057800 Rorug06G0082000 Rorug06G0118900 Rorug06G0176500 Rorug06G0423100 Rorug07G0050100 Rorug07G0095100 Rorug07G0202100 Rorug07G0216600 Rorug07G0244300 Rorug07G0245500
rosa_samantha Rh1DG154800 Rh2CG613000 Rh6BG016600 Rh6BG475100 Rh6CG013200 Rh7AG443800
rosa_wichuraiana Rw0G021840 Rw4G023650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3674
Acc16I TGCGCA 1 cut(s) 1781
Acc36I ACCTGC 1 cut(s) 3674
AccB1I GGYRCC 2 cut(s) 1238, 2510
AccI GTMKAC 2 cut(s) 60, 1374
AciI CCGC 8 cut(s) 364, 1766, 3579, 3613, 3706, 3878, 3934, 3972
AcoI YGGCCR 2 cut(s) 592, 740
AcsI RAATTY 9 cut(s) 104, 230, 273, 613, 682, 1044, 1281, 2230, 3551
AcuI CTGAAG 4 cut(s) 719, 801, 891, 3785
AcvI CACGTG 1 cut(s) 449
AdeI CACNNNGTG 4 cut(s) 260, 1934, 2459, 3026
AfaI GTAC 5 cut(s) 493, 503, 522, 1075, 3544
AfiI CCNNNNNNNGG 7 cut(s) 505, 2871, 2882, 3106, 3211, 3923, 3943
AflIII ACRYGT 1 cut(s) 1123
AhlI ACTAGT 2 cut(s) 517, 1840
AjnI CCWGG 8 cut(s) 1000, 1984, 2864, 2875, 2962, 3022, 3078, 3118
AjuI GAANNNNNNNTTGG 6 cut(s) 33, 65, 573, 605, 1565, 1597
Alw21I GWGCWC 6 cut(s) 1090, 2321, 3442, 3691, 3785, 3872
Alw26I GTCTC 7 cut(s) 564, 767, 1628, 2120, 3045, 3512, 3742
Alw44I GTGCAC 2 cut(s) 1086, 3687
AlwNI CAGNNNCTG 4 cut(s) 1416, 1537, 2794, 2981
AoxI GGCC 7 cut(s) 592, 740, 1255, 1999, 2571, 2873, 3177
ApaLI GTGCAC 2 cut(s) 1086, 3687
ApeKI GCWGC 9 cut(s) 818, 839, 1232, 1962, 2269, 2348, 2351, 3610, 3680
ApoI RAATTY 9 cut(s) 104, 230, 273, 613, 682, 1044, 1281, 2230, 3551
ArsI GACNNNNNNTTYG 8 cut(s) 425, 457, 1084, 1116, 3490, 3522, 3720, 3752
AseI ATTAAT 1 cut(s) 3360
Asp700I GAANNNNTTC 3 cut(s) 309, 1216, 2230
AspLEI GCGC 2 cut(s) 1782, 3823
AspS9I GGNCC 5 cut(s) 1661, 2406, 2501, 2591, 2873
AsuC2I CCSGG 1 cut(s) 3944
AsuII TTCGAA 1 cut(s) 3425
AvaII GGWCC 4 cut(s) 1661, 2406, 2501, 2591
BaeGI GKGCMC 2 cut(s) 1090, 3691
BalI TGGCCA 2 cut(s) 594, 742
BamHI GGATCC 4 cut(s) 649, 1157, 3000, 3244
BanI GGYRCC 2 cut(s) 1238, 2510
BanII GRGCYC 1 cut(s) 1798
BbrPI CACGTG 1 cut(s) 449
BbsI GAAGAC 6 cut(s) 76, 3051, 3081, 3175, 3666, 3735
Bbv12I GWGCWC 6 cut(s) 1090, 2321, 3442, 3691, 3785, 3872
BbvI GCAGC 9 cut(s) 805, 851, 1244, 1949, 2281, 2360, 2363, 3597, 3692
BceAI ACGGC 1 cut(s) 3944
BcgI CGANNNNNNTGC 2 cut(s) 2258, 2292
BciT130I CCWGG 8 cut(s) 1002, 1986, 2866, 2877, 2964, 3024, 3080, 3120
BclI TGATCA 1 cut(s) 2701
BcnI CCSGG 1 cut(s) 3944
BcoDI GTCTC 7 cut(s) 564, 767, 1628, 2120, 3045, 3512, 3742
BcuI ACTAGT 2 cut(s) 517, 1840
BfmI CTRYAG 4 cut(s) 546, 819, 1848, 3883
BfuAI ACCTGC 1 cut(s) 3674
BglI GCCNNNNNGGC 2 cut(s) 2360, 3185
BglII AGATCT 2 cut(s) 1871, 3617
BlpI GCTNAGC 2 cut(s) 324, 1630
Bme1390I CCNGG 9 cut(s) 1002, 1986, 2866, 2877, 2964, 3024, 3080, 3120, 3944
Bme18I GGWCC 4 cut(s) 1661, 2406, 2501, 2591
BmgT120I GGNCC 5 cut(s) 1661, 2406, 2501, 2591, 2873
BmrFI CCNGG 9 cut(s) 1002, 1986, 2866, 2877, 2964, 3024, 3080, 3120, 3944
BmrI ACTGGG 2 cut(s) 93, 1426
BmuI ACTGGG 2 cut(s) 93, 1426
BpiI GAAGAC 6 cut(s) 76, 3051, 3081, 3175, 3666, 3735
BplI GAGNNNNNCTC 4 cut(s) 2057, 2089, 3879, 3911
BpmI CTGGAG 6 cut(s) 809, 1662, 2412, 2588, 2946, 3398
Bpu10I CCTNAGC 1 cut(s) 2596
Bpu1102I GCTNAGC 2 cut(s) 324, 1630
Bpu14I TTCGAA 1 cut(s) 3425
BpuEI CTTGAG 3 cut(s) 1782, 1940, 2664
BpuMI CCSGG 1 cut(s) 3944
Bsa29I ATCGAT 1 cut(s) 3520
BsaAI YACGTR 1 cut(s) 449
BsaBI GATNNNNATC 1 cut(s) 2971
BsaI GGTCTC 2 cut(s) 564, 3512
BsaJI CCNNGG 4 cut(s) 1001, 2865, 3079, 3942
BsaWI WCCGGW 1 cut(s) 2309
BsaXI ACNNNNNCTCC 4 cut(s) 2385, 2415, 3953, 3983
Bsc4I CCNNNNNNNGG 7 cut(s) 505, 2871, 2882, 3106, 3211, 3923, 3943
Bse118I RCCGGY 1 cut(s) 3456
Bse3DI GCAATG 5 cut(s) 105, 341, 459, 1377, 3580
Bse8I GATNNNNATC 1 cut(s) 2971
BseBI CCWGG 8 cut(s) 1002, 1986, 2866, 2877, 2964, 3024, 3080, 3120
BseCI ATCGAT 1 cut(s) 3520
BseDI CCNNGG 4 cut(s) 1001, 2865, 3079, 3942
BseJI GATNNNNATC 1 cut(s) 2971
BseLI CCNNNNNNNGG 7 cut(s) 505, 2871, 2882, 3106, 3211, 3923, 3943
BseMI GCAATG 5 cut(s) 105, 341, 459, 1377, 3580
BseMII CTCAG 6 cut(s) 1368, 2570, 3000, 3645, 3798, 3897
BseRI GAGGAG 5 cut(s) 1007, 1821, 2667, 2810, 3759
BseSI GKGCMC 2 cut(s) 1090, 3691
BseXI GCAGC 9 cut(s) 805, 851, 1244, 1949, 2281, 2360, 2363, 3597, 3692
BseYI CCCAGC 1 cut(s) 3627
BsgI GTGCAG 1 cut(s) 2367
Bsh1285I CGRYCG 1 cut(s) 3274
BshFI GGCC 7 cut(s) 594, 742, 1257, 2001, 2573, 2875, 3179
BshNI GGYRCC 2 cut(s) 1238, 2510
BshVI ATCGAT 1 cut(s) 3520
BsiEI CGRYCG 1 cut(s) 3274
BsiHKAI GWGCWC 6 cut(s) 1090, 2321, 3442, 3691, 3785, 3872
BsiSI CCGG 3 cut(s) 2310, 3457, 3944
BslFI GGGAC 1 cut(s) 2365
BslI CCNNNNNNNGG 7 cut(s) 505, 2871, 2882, 3106, 3211, 3923, 3943
BsmAI GTCTC 7 cut(s) 564, 767, 1628, 2120, 3045, 3512, 3742
BsmFI GGGAC 1 cut(s) 2365
BsmI GAATGC 4 cut(s) 1989, 2176, 2468, 2962
BsnI GGCC 7 cut(s) 594, 742, 1257, 2001, 2573, 2875, 3179
Bso31I GGTCTC 2 cut(s) 564, 3512
Bsp119I TTCGAA 1 cut(s) 3425
Bsp1286I GDGCHC 7 cut(s) 1090, 1798, 2321, 3442, 3691, 3785, 3872
Bsp1407I TGTACA 1 cut(s) 501
Bsp1720I GCTNAGC 2 cut(s) 324, 1630
BspACI CCGC 8 cut(s) 364, 1766, 3579, 3613, 3706, 3878, 3934, 3972
BspANI GGCC 7 cut(s) 594, 742, 1257, 2001, 2573, 2875, 3179
BspCNI CTCAG 6 cut(s) 1367, 2571, 2999, 3646, 3797, 3898
BspDI ATCGAT 1 cut(s) 3520
BspMAI CTGCAG 3 cut(s) 550, 823, 3887
BspMI ACCTGC 1 cut(s) 3674
BspQI GCTCTTC 2 cut(s) 2135, 3600
BspT104I TTCGAA 1 cut(s) 3425
BspT107I GGYRCC 2 cut(s) 1238, 2510
BspTNI GGTCTC 2 cut(s) 564, 3512
BsrDI GCAATG 5 cut(s) 105, 341, 459, 1377, 3580
BsrFI RCCGGY 1 cut(s) 3456
BsrGI TGTACA 1 cut(s) 501
BssAI RCCGGY 1 cut(s) 3456
BssECI CCNNGG 4 cut(s) 1001, 2865, 3079, 3942
BssNAI GTATAC 1 cut(s) 61
Bst1107I GTATAC 1 cut(s) 61
Bst2UI CCWGG 8 cut(s) 1002, 1986, 2866, 2877, 2964, 3024, 3080, 3120
Bst6I CTCTTC 3 cut(s) 1471, 2135, 3600
BstAPI GCANNNNNTGC 5 cut(s) 1238, 2201, 3453, 3686, 3760
BstAUI TGTACA 1 cut(s) 501
BstBAI YACGTR 1 cut(s) 449
BstBI TTCGAA 1 cut(s) 3425
BstEII GGTNACC 1 cut(s) 3200
BstENI CCTNNNNNAGG 2 cut(s) 2880, 3104
BstHHI GCGC 2 cut(s) 1782, 3823
BstMAI GTCTC 7 cut(s) 564, 767, 1628, 2120, 3045, 3512, 3742
BstMCI CGRYCG 1 cut(s) 3274
BstNI CCWGG 8 cut(s) 1002, 1986, 2866, 2877, 2964, 3024, 3080, 3120
BstNSI RCATGY 3 cut(s) 1127, 1698, 2845
BstPI GGTNACC 1 cut(s) 3200
BstSCI CCNGG 9 cut(s) 1000, 1984, 2864, 2875, 2962, 3022, 3078, 3118, 3942
BstSFI CTRYAG 4 cut(s) 546, 819, 1848, 3883
BstSLI GKGCMC 2 cut(s) 1090, 3691
BstV1I GCAGC 9 cut(s) 805, 851, 1244, 1949, 2281, 2360, 2363, 3597, 3692
BstV2I GAAGAC 6 cut(s) 76, 3051, 3081, 3175, 3666, 3735
BstX2I RGATCY 8 cut(s) 649, 1157, 1871, 2110, 3000, 3244, 3409, 3617
BstXI CCANNNNNNTGG 1 cut(s) 2167
BstYI RGATCY 8 cut(s) 649, 1157, 1871, 2110, 3000, 3244, 3409, 3617
BstZ17I GTATAC 1 cut(s) 61
Bsu15I ATCGAT 1 cut(s) 3520
BsuRI GGCC 7 cut(s) 594, 742, 1257, 2001, 2573, 2875, 3179
BsuTUI ATCGAT 1 cut(s) 3520
BtsI GCAGTG 5 cut(s) 543, 1465, 1575, 2433, 3442
BveI ACCTGC 1 cut(s) 3674
CaiI CAGNNNCTG 4 cut(s) 1416, 1537, 2794, 2981
CfoI GCGC 2 cut(s) 1782, 3823
Cfr10I RCCGGY 1 cut(s) 3456
Cfr13I GGNCC 5 cut(s) 1661, 2406, 2501, 2591, 2873
ClaI ATCGAT 1 cut(s) 3520
CseI GACGC 1 cut(s) 81
CsiI ACCWGGT 1 cut(s) 3022
Csp6I GTAC 5 cut(s) 492, 502, 521, 1074, 3543
CspCI CAANNNNNGTGG 2 cut(s) 1285, 1320
CviQI GTAC 5 cut(s) 492, 502, 521, 1074, 3543
DraI TTTAAA 2 cut(s) 688, 2214
DraIII CACNNNGTG 4 cut(s) 260, 1934, 2459, 3026
EaeI YGGCCR 2 cut(s) 592, 740
Eam1104I CTCTTC 3 cut(s) 1471, 2135, 3600
EarI CTCTTC 3 cut(s) 1471, 2135, 3600
EciI GGCGGA 3 cut(s) 3867, 3923, 3961
Eco24I GRGCYC 1 cut(s) 1798
Eco31I GGTCTC 2 cut(s) 564, 3512
Eco32I GATATC 2 cut(s) 139, 2835
Eco47I GGWCC 4 cut(s) 1661, 2406, 2501, 2591
Eco57I CTGAAG 4 cut(s) 719, 801, 891, 3785
Eco72I CACGTG 1 cut(s) 449
Eco91I GGTNACC 1 cut(s) 3200
EcoNI CCTNNNNNAGG 2 cut(s) 2880, 3104
EcoO109I RGGNCCY 4 cut(s) 1661, 2406, 2501, 2591
EcoO65I GGTNACC 1 cut(s) 3200
EcoRI GAATTC 1 cut(s) 230
EcoRII CCWGG 8 cut(s) 1000, 1984, 2864, 2875, 2962, 3022, 3078, 3118
EcoRV GATATC 2 cut(s) 139, 2835
EcoT22I ATGCAT 3 cut(s) 1471, 1700, 2847
EcoT38I GRGCYC 1 cut(s) 1798
FalI AAGNNNNNCTT 4 cut(s) 1173, 1205, 2697, 2729
FaqI GGGAC 1 cut(s) 2365
FbaI TGATCA 1 cut(s) 2701
FblI GTMKAC 2 cut(s) 60, 1374
FriOI GRGCYC 1 cut(s) 1798
FspI TGCGCA 1 cut(s) 1781
GlaI GCGC 2 cut(s) 1781, 3822
GsaI CCCAGC 1 cut(s) 3631
GsuI CTGGAG 6 cut(s) 809, 1662, 2412, 2588, 2946, 3398
HaeIII GGCC 7 cut(s) 594, 742, 1257, 2001, 2573, 2875, 3179
HapII CCGG 3 cut(s) 2310, 3457, 3944
HgaI GACGC 1 cut(s) 81
HhaI GCGC 2 cut(s) 1782, 3823
Hin6I GCGC 2 cut(s) 1780, 3821
HinP1I GCGC 2 cut(s) 1780, 3821
HincII GTYRAC 1 cut(s) 1518
HindII GTYRAC 1 cut(s) 1518
HpaI GTTAAC 1 cut(s) 1518
HpaII CCGG 3 cut(s) 2310, 3457, 3944
HpyCH4IV ACGT 4 cut(s) 448, 1678, 1915, 3541
HpySE526I ACGT 4 cut(s) 448, 1678, 1915, 3541
HspAI GCGC 2 cut(s) 1780, 3821
KroI GCCGGC 1 cut(s) 3456
KroNI GCCGGC 1 cut(s) 3458
Ksp22I TGATCA 1 cut(s) 2701
KspAI GTTAAC 1 cut(s) 1518
LguI GCTCTTC 2 cut(s) 2135, 3600
LmnI GCTCC 8 cut(s) 514, 1643, 2393, 2771, 2965, 3470, 3746, 3936
Lsp1109I GCAGC 9 cut(s) 805, 851, 1244, 1949, 2281, 2360, 2363, 3597, 3692
MabI ACCWGGT 1 cut(s) 3022
MaeII ACGT 4 cut(s) 448, 1678, 1915, 3541
MaeIII GTNAC 8 cut(s) 241, 377, 431, 1674, 1852, 2020, 3200, 3828
MfeI CAATTG 4 cut(s) 318, 329, 2121, 3954
MflI RGATCY 8 cut(s) 649, 1157, 1871, 2110, 3000, 3244, 3409, 3617
MhlI GDGCHC 7 cut(s) 1090, 1798, 2321, 3442, 3691, 3785, 3872
MlsI TGGCCA 2 cut(s) 594, 742
MluNI TGGCCA 2 cut(s) 594, 742
MlyI GAGTC 3 cut(s) 956, 1499, 3836
MmeI TCCRAC 3 cut(s) 3091, 3222, 3738
Mox20I TGGCCA 2 cut(s) 594, 742
Mph1103I ATGCAT 3 cut(s) 1471, 1700, 2847
MroNI GCCGGC 1 cut(s) 3456
MroXI GAANNNNTTC 3 cut(s) 309, 1216, 2230
MscI TGGCCA 2 cut(s) 594, 742
MslI CAYNNNNRTG 1 cut(s) 200
Msp20I TGGCCA 2 cut(s) 594, 742
MspA1I CMGCKG 1 cut(s) 1235
MspI CCGG 3 cut(s) 2310, 3457, 3944
MspR9I CCNGG 9 cut(s) 1002, 1986, 2866, 2877, 2964, 3024, 3080, 3120, 3944
MunI CAATTG 4 cut(s) 318, 329, 2121, 3954
Mva1269I GAATGC 4 cut(s) 1989, 2176, 2468, 2962
MvaI CCWGG 8 cut(s) 1002, 1986, 2866, 2877, 2964, 3024, 3080, 3120
NaeI GCCGGC 1 cut(s) 3458
NciI CCSGG 1 cut(s) 3944
NgoMIV GCCGGC 1 cut(s) 3456
NmuCI GTSAC 6 cut(s) 241, 377, 431, 1674, 3200, 3828
NsbI TGCGCA 1 cut(s) 1781
NsiI ATGCAT 3 cut(s) 1471, 1700, 2847
NspI RCATGY 3 cut(s) 1127, 1698, 2845
NspV TTCGAA 1 cut(s) 3425
PaeI GCATGC 1 cut(s) 1698
PaqCI CACCTGC 1 cut(s) 3674
PciI ACATGT 1 cut(s) 1123
PciSI GCTCTTC 2 cut(s) 2135, 3600
PctI GAATGC 4 cut(s) 1989, 2176, 2468, 2962
PdiI GCCGGC 1 cut(s) 3458
PdmI GAANNNNTTC 3 cut(s) 309, 1216, 2230
PflFI GACNNNGTC 1 cut(s) 3734
PfoI TCCNGGA 1 cut(s) 2962
Ple19I CGATCG 1 cut(s) 3274
PleI GAGTC 3 cut(s) 956, 1498, 3835
PmaCI CACGTG 1 cut(s) 449
PmlI CACGTG 1 cut(s) 449
PpsI GAGTC 3 cut(s) 956, 1498, 3835
Ppu21I YACGTR 1 cut(s) 449
PpuMI RGGWCCY 4 cut(s) 1661, 2406, 2501, 2591
PscI ACATGT 1 cut(s) 1123
PshBI ATTAAT 1 cut(s) 3360
Psp5II RGGWCCY 4 cut(s) 1661, 2406, 2501, 2591
Psp6I CCWGG 8 cut(s) 1000, 1984, 2864, 2875, 2962, 3022, 3078, 3118
PspCI CACGTG 1 cut(s) 449
PspEI GGTNACC 1 cut(s) 3200
PspFI CCCAGC 1 cut(s) 3627
PspGI CCWGG 8 cut(s) 1000, 1984, 2864, 2875, 2962, 3022, 3078, 3118
PspPI GGNCC 5 cut(s) 1661, 2406, 2501, 2591, 2873
PspPPI RGGWCCY 4 cut(s) 1661, 2406, 2501, 2591
PstI CTGCAG 3 cut(s) 550, 823, 3887
PstNI CAGNNNCTG 4 cut(s) 1416, 1537, 2794, 2981
PsuI RGATCY 8 cut(s) 649, 1157, 1871, 2110, 3000, 3244, 3409, 3617
PsyI GACNNNGTC 1 cut(s) 3734
PvuI CGATCG 1 cut(s) 3274
PvuII CAGCTG 1 cut(s) 1235
RsaI GTAC 5 cut(s) 493, 503, 522, 1075, 3544
RsaNI GTAC 5 cut(s) 492, 502, 521, 1074, 3543
RseI CAYNNNNRTG 1 cut(s) 200
SapI GCTCTTC 2 cut(s) 2135, 3600
Sau96I GGNCC 5 cut(s) 1661, 2406, 2501, 2591, 2873
SchI GAGTC 3 cut(s) 956, 1499, 3836
ScrFI CCNGG 9 cut(s) 1002, 1986, 2866, 2877, 2964, 3024, 3080, 3120, 3944
SduI GDGCHC 7 cut(s) 1090, 1798, 2321, 3442, 3691, 3785, 3872
SexAI ACCWGGT 1 cut(s) 3022
SfcI CTRYAG 4 cut(s) 546, 819, 1848, 3883
SfuI TTCGAA 1 cut(s) 3425
SinI GGWCC 4 cut(s) 1661, 2406, 2501, 2591
SmiMI CAYNNNNRTG 1 cut(s) 200
SmlI CTYRAG 3 cut(s) 1797, 1919, 2679
SmoI CTYRAG 3 cut(s) 1797, 1919, 2679
SpeI ACTAGT 2 cut(s) 517, 1840
SphI GCATGC 1 cut(s) 1698
SsiI CCGC 8 cut(s) 364, 1766, 3579, 3613, 3706, 3878, 3934, 3972
SspI AATATT 1 cut(s) 955
StyD4I CCNGG 9 cut(s) 1000, 1984, 2864, 2875, 2962, 3022, 3078, 3118, 3942
TaiI ACGT 4 cut(s) 451, 1681, 1918, 3544
TatI WGTACW 1 cut(s) 501
TauI GCSGC 1 cut(s) 3581
TseFI GTSAC 6 cut(s) 241, 377, 431, 1674, 3200, 3828
TseI GCWGC 9 cut(s) 818, 839, 1232, 1962, 2269, 2348, 2351, 3610, 3680
Tsp45I GTSAC 6 cut(s) 241, 377, 431, 1674, 3200, 3828
TspGWI ACGGA 2 cut(s) 3562, 3887
Tth111I GACNNNGTC 1 cut(s) 3734
VneI GTGCAC 2 cut(s) 1086, 3687
VpaK11BI GGWCC 4 cut(s) 1661, 2406, 2501, 2591
VspI ATTAAT 1 cut(s) 3360
XagI CCTNNNNNAGG 2 cut(s) 2880, 3104
XapI RAATTY 9 cut(s) 104, 230, 273, 613, 682, 1044, 1281, 2230, 3551
XbaI TCTAGA 2 cut(s) 3139, 3740
XceI RCATGY 3 cut(s) 1127, 1698, 2845
XcmI CCANNNNNNNNNTGG 3 cut(s) 875, 1303, 2362
XmiI GTMKAC 2 cut(s) 60, 1374
XmnI GAANNNNTTC 3 cut(s) 309, 1216, 2230
Zsp2I ATGCAT 3 cut(s) 1471, 1700, 2847
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.