RchiOBHm_Chr2g0125461

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000254
Physical Location & Seq
Reverse (-)
39196924 .. 39197709
786 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49757

Sequence Viewer

Length: 786 bp
ATGATGAACACCTTTAATTTAAATTGGGAGGAATGGATTTTAGCTAACTTGATGCAAAAAGGTTACTACAAGGAAAATTTGAGCTGGAACATTTTCTTCATTTTCTGCTGCTGGTTTTTGTGGAAATGGAGGTGTAAAAGAGTTTTCGATGTGAATTTTTGTTATCCTCATAATCCTCCTGAGATTGTTTTTAGCTATGCTACTGAATGGTTTAAGGCTACTGTTAAGCCTAATGTGAACATGAATTGTCATGTTGAAATGCTTTCTTGGATTAAACCTGCTATGGGAAACCATAAACTTAATGTGGATGGCTCAAGAGCAAACAATGGCTCAATTGGAGCAGGTGGTGTTATAAGAGATGATTCAGGTTGCTGGTGTGGTGGTTTTATGGTTAACATTGGTACTGGTGAAGTTCTACAAGCTGAAGCCTGGGGTTTATATCATGGCCTTCACCTTGCTTTGAGTCTAAATATTACAAGATTGGAAGTTGAATCAGATTCTTCTATTTTGATTAGTCTCATCCACAGTGACAATGTTGGCTTACATCCTTTGGGGACTTTGATCATGAACTGCAGAAACCTCTTGAAAGGCTTTGACTTTATTCAGATTAAGCATGTTCATCGTGAACGTAATATGGTTGCTGATTTATTAGCCAAGAATAGTACTTCGCATGCCAAAGGAGTCTGCATTTTTCATGATCCTCCTGCGTTAGTGACTGAAGACCTTTTAGATGATATTGTGGGAGCTCCTAGAGCTAGAAGATTTAATGCCAGCAGTGCTGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

261

Amino Acids

29.55

Weight (kDa)

6.82

Isoelectric Point (pI)

32.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 101 - 220 7.9e-26 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000254)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25671 FvH4_1g29782 FvH4_2g06731 FvH4_2g09413 FvH4_2g12061 FvH4_2g12671 FvH4_2g14202 FvH4_3g27901 FvH4_3g30492 FvH4_3g36953 FvH4_4g10141 FvH4_4g11162 FvH4_5g27995 FvH4_5g34262 FvH4_6g10681 FvH4_6g13733 FvH4_6g18164 FvH4_6g25491 FvH4_6g25791 FvH4_6g27023 FvH4_6g34321 FvH4_7g05070
pyrus_communis pycom04g06850
rosa_chinensis RchiOBHm_Chr1g0322281 RchiOBHm_Chr1g0334981 RchiOBHm_Chr1g0371181 RchiOBHm_Chr2g0125461 RchiOBHm_Chr2g0127141 RchiOBHm_Chr2g0128961 RchiOBHm_Chr2g0132921 RchiOBHm_Chr3g0486471 RchiOBHm_Chr3g0492911 RchiOBHm_Chr4g0412131 RchiOBHm_Chr4g0426941 RchiOBHm_Chr4g0437331 RchiOBHm_Chr5g0018821 RchiOBHm_Chr5g0040021 RchiOBHm_Chr5g0051041 RchiOBHm_Chr6g0266871 RchiOBHm_Chr6g0271761 RchiOBHm_Chr7g0200931 RchiOBHm_Chr7g0220091
rosa_multiflora Rmu_co7991344.1_g000001 Rmu_sc0000172.1_g000008 Rmu_sc0000194.1_g000010 Rmu_sc0000446.1_g000026 Rmu_sc0000496.1_g000011 Rmu_sc0000530.1_g000013 Rmu_sc0000706.1_g000044 Rmu_sc0000745.1_g000029 Rmu_sc0001301.1_g000027 Rmu_sc0001520.1_g000008 Rmu_sc0001966.1_g000025 Rmu_sc0001989.1_g000007 Rmu_sc0002239.1_g000002 Rmu_sc0002816.1_g000004 Rmu_sc0003458.1_g000001 Rmu_sc0003825.1_g000031 Rmu_sc0003919.1_g000002 Rmu_sc0004053.1_g000007 Rmu_sc0004161.1_g000008 Rmu_sc0004368.1_g000033 Rmu_sc0004383.1_g000002 Rmu_sc0004923.1_g000002 Rmu_sc0005065.1_g000001 Rmu_sc0005478.1_g000001 Rmu_sc0006847.1_g000031 Rmu_sc0009556.1_g000002 Rmu_sc0010783.1_g000007 Rmu_sc0011424.1_g000022 Rmu_sc0013205.1_g000002 Rmu_sc0017407.1_g000001 Rmu_sc0023757.1_g000002 Rmu_sc0032346.1_g000001 Rmu_ssc0000008.1_g000014 Rmu_ssc0000158.1_g000033 Rmu_ssc0000217.1_g000028
rosa_roxburghii Rroxscaffold_1G00000480 Rroxscaffold_1G00026050 Rroxscaffold_1G00026250 Rroxscaffold_2G00085120 Rroxscaffold_2G00119710 Rroxscaffold_2G00126610 Rroxscaffold_2G00129340 Rroxscaffold_3G00245550 Rroxscaffold_5G00340210 Rroxscaffold_5G00340330 Rroxscaffold_6G00397180 Rroxscaffold_7G00216160
rosa_rugosa Rorug01G0040000 Rorug01G0067400 Rorug01G0092300 Rorug01G0197000 Rorug01G0474200 Rorug01G0482500 Rorug01G0489300 Rorug02G0142900 Rorug02G0259000 Rorug02G0283200 Rorug02G0341400 Rorug02G0341600 Rorug02G0370900 Rorug02G0379700 Rorug02G0386100 Rorug02G0387300 Rorug03G0112600 Rorug03G0265600 Rorug03G0298800 Rorug04G0019500 Rorug05G0069900 Rorug05G0070500 Rorug05G0256800 Rorug05G0287300 Rorug05G0592000 Rorug06G0057800 Rorug06G0082000 Rorug06G0118900 Rorug06G0176500 Rorug06G0423100 Rorug07G0050100 Rorug07G0095100 Rorug07G0202100 Rorug07G0216600 Rorug07G0244300 Rorug07G0245500
rosa_samantha Rh1DG154800 Rh2CG613000 Rh6BG016600 Rh6BG475100 Rh6CG013200 Rh7AG443800
rosa_wichuraiana Rw0G021840 Rw4G023650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 353
AarI CACCTGC 1 cut(s) 332
Acc36I ACCTGC 2 cut(s) 286, 332
AclWI GGATC 1 cut(s) 692
AcsI RAATTY 2 cut(s) 76, 154
AcuI CTGAAG 2 cut(s) 444, 738
AfaI GTAC 2 cut(s) 403, 664
AfiI CCNNNNNNNGG 1 cut(s) 284
AgsI TTSAA 3 cut(s) 257, 491, 586
AjnI CCWGG 1 cut(s) 428
AluBI AGCT 6 cut(s) 44, 84, 195, 422, 746, 755
AluI AGCT 6 cut(s) 44, 84, 195, 422, 746, 755
Alw21I GWGCWC 1 cut(s) 748
Alw26I GTCTC 1 cut(s) 521
AlwI GGATC 1 cut(s) 692
AoxI GGCC 1 cut(s) 445
ApeKI GCWGC 1 cut(s) 108
ApoI RAATTY 2 cut(s) 76, 154
Asp700I GAANNNNTTC 1 cut(s) 92
AsuHPI GGTGA 2 cut(s) 419, 443
BanII GRGCYC 1 cut(s) 748
BbsI GAAGAC 1 cut(s) 726
Bbv12I GWGCWC 1 cut(s) 748
BbvI GCAGC 1 cut(s) 95
BccI CCATC 1 cut(s) 302
BcgI CGANNNNNNTGC 2 cut(s) 602, 636
BciT130I CCWGG 1 cut(s) 430
BclI TGATCA 1 cut(s) 561
BcoDI GTCTC 1 cut(s) 521
BfaI CTAG 3 cut(s) 750, 756, 784
BfmI CTRYAG 1 cut(s) 571
BfuAI ACCTGC 2 cut(s) 286, 332
BisI GCNGC 1 cut(s) 109
BlsI GCNGC 1 cut(s) 110
BmcAI AGTACT 1 cut(s) 664
Bme1390I CCNGG 1 cut(s) 430
BmrFI CCNGG 1 cut(s) 430
BmsI GCATC 1 cut(s) 42
BpiI GAAGAC 1 cut(s) 726
BpuEI CTTGAG 1 cut(s) 298
BsaJI CCNNGG 1 cut(s) 429
Bsc4I CCNNNNNNNGG 1 cut(s) 284
Bse1I ACTGG 1 cut(s) 409
BseBI CCWGG 1 cut(s) 430
BseDI CCNNGG 1 cut(s) 429
BseGI GGATG 3 cut(s) 313, 519, 544
BseLI CCNNNNNNNGG 1 cut(s) 284
BseMII CTCAG 1 cut(s) 171
BseNI ACTGG 1 cut(s) 409
BseXI GCAGC 1 cut(s) 95
BshFI GGCC 1 cut(s) 447
BsiHKAI GWGCWC 1 cut(s) 748
BslFI GGGAC 1 cut(s) 568
BslI CCNNNNNNNGG 1 cut(s) 284
BsmAI GTCTC 1 cut(s) 521
BsmFI GGGAC 1 cut(s) 568
BsnI GGCC 1 cut(s) 447
Bsp1286I GDGCHC 1 cut(s) 748
Bsp143I GATC 2 cut(s) 561, 697
BspANI GGCC 1 cut(s) 447
BspCNI CTCAG 1 cut(s) 172
BspHI TCATGA 2 cut(s) 564, 694
BspMAI CTGCAG 1 cut(s) 575
BspMI ACCTGC 2 cut(s) 286, 332
BspPI GGATC 1 cut(s) 692
BsrI ACTGG 1 cut(s) 409
BssECI CCNNGG 1 cut(s) 429
BssMI GATC 2 cut(s) 561, 697
Bst2UI CCWGG 1 cut(s) 430
Bst4CI ACNGT 2 cut(s) 223, 527
BstC8I GCNNGC 3 cut(s) 672, 772, 781
BstDEI CTNAG 1 cut(s) 180
BstF5I GGATG 3 cut(s) 313, 519, 544
BstKTI GATC 2 cut(s) 564, 700
BstMAI GTCTC 1 cut(s) 521
BstMBI GATC 2 cut(s) 561, 697
BstMWI GCNNNNNNNGC 3 cut(s) 752, 776, 780
BstNI CCWGG 1 cut(s) 430
BstNSI RCATGY 2 cut(s) 617, 674
BstSCI CCNGG 1 cut(s) 428
BstSFI CTRYAG 1 cut(s) 571
BstV1I GCAGC 1 cut(s) 95
BstV2I GAAGAC 1 cut(s) 726
BsuRI GGCC 1 cut(s) 447
BtsCI GGATG 3 cut(s) 313, 519, 544
BtsI GCAGTG 1 cut(s) 781
BtsIMutI CAGTG 2 cut(s) 532, 781
BveI ACCTGC 2 cut(s) 286, 332
Cac8I GCNNGC 3 cut(s) 672, 772, 781
CciI TCATGA 2 cut(s) 564, 694
Csp6I GTAC 2 cut(s) 402, 663
CviAII CATG 7 cut(s) 241, 251, 443, 565, 614, 671, 695
CviQI GTAC 2 cut(s) 402, 663
DdeI CTNAG 1 cut(s) 180
DpnI GATC 2 cut(s) 563, 699
DpnII GATC 2 cut(s) 561, 697
DraI TTTAAA 1 cut(s) 21
Ecl136II GAGCTC 1 cut(s) 746
Eco24I GRGCYC 1 cut(s) 748
Eco53kI GAGCTC 1 cut(s) 746
Eco57I CTGAAG 2 cut(s) 444, 738
EcoICRI GAGCTC 1 cut(s) 746
EcoRII CCWGG 1 cut(s) 428
EcoT38I GRGCYC 1 cut(s) 748
FaeI CATG 7 cut(s) 244, 254, 446, 568, 617, 674, 698
FaqI GGGAC 1 cut(s) 568
FatI CATG 7 cut(s) 240, 250, 442, 564, 613, 670, 694
FbaI TGATCA 1 cut(s) 561
Fnu4HI GCNGC 1 cut(s) 109
FokI GGATG 3 cut(s) 320, 506, 531
FriOI GRGCYC 1 cut(s) 748
Fsp4HI GCNGC 1 cut(s) 109
FspBI CTAG 3 cut(s) 750, 756, 784
GluI GCNGC 1 cut(s) 109
HaeIII GGCC 1 cut(s) 447
Hin1II CATG 7 cut(s) 244, 254, 446, 568, 617, 674, 698
HincII GTYRAC 1 cut(s) 394
HindII GTYRAC 1 cut(s) 394
HinfI GANTC 5 cut(s) 362, 463, 491, 497, 681
HpaI GTTAAC 1 cut(s) 394
HphI GGTGA 2 cut(s) 419, 443
Hpy166II GTNNAC 3 cut(s) 238, 394, 626
Hpy188I TCNGA 2 cut(s) 496, 606
Hpy188III TCNNGA 6 cut(s) 179, 315, 565, 583, 623, 695
Hpy8I GTNNAC 3 cut(s) 238, 394, 626
HpyAV CCTTC 1 cut(s) 458
HpyCH4III ACNGT 2 cut(s) 223, 527
HpyCH4IV ACGT 1 cut(s) 628
HpyCH4V TGCA 3 cut(s) 55, 573, 687
HpyF10VI GCNNNNNNNGC 3 cut(s) 752, 776, 780
HpyF3I CTNAG 1 cut(s) 180
HpySE526I ACGT 1 cut(s) 628
Hsp92II CATG 7 cut(s) 244, 254, 446, 568, 617, 674, 698
Ksp22I TGATCA 1 cut(s) 561
KspAI GTTAAC 1 cut(s) 394
Kzo9I GATC 2 cut(s) 561, 697
LmnI GCTCC 3 cut(s) 338, 743, 751
Lsp1109I GCAGC 1 cut(s) 95
LweI GCATC 1 cut(s) 42
MaeI CTAG 3 cut(s) 750, 756, 784
MaeII ACGT 1 cut(s) 628
MaeIII GTNAC 3 cut(s) 62, 527, 712
MalI GATC 2 cut(s) 563, 699
MboI GATC 2 cut(s) 561, 697
MboII GAAGA 4 cut(s) 88, 492, 731, 771
MfeI CAATTG 1 cut(s) 333
MhlI GDGCHC 1 cut(s) 748
MluCI AATT 6 cut(s) 16, 22, 76, 154, 244, 333
MlyI GAGTC 2 cut(s) 472, 690
MnlI CCTC 6 cut(s) 22, 123, 177, 186, 590, 711
MroXI GAANNNNTTC 1 cut(s) 92
MseI TTAA 9 cut(s) 15, 20, 213, 225, 273, 300, 393, 609, 765
MspR9I CCNGG 1 cut(s) 430
MunI CAATTG 1 cut(s) 333
MvaI CCWGG 1 cut(s) 430
MwoI GCNNNNNNNGC 3 cut(s) 752, 776, 780
NdeII GATC 2 cut(s) 561, 697
NlaIII CATG 7 cut(s) 244, 254, 446, 568, 617, 674, 698
NmuCI GTSAC 2 cut(s) 527, 712
NspI RCATGY 2 cut(s) 617, 674
PaeI GCATGC 1 cut(s) 674
PagI TCATGA 2 cut(s) 564, 694
PaqCI CACCTGC 1 cut(s) 332
PdmI GAANNNNTTC 1 cut(s) 92
PfeI GAWTC 3 cut(s) 362, 491, 497
PkrI GCNGC 1 cut(s) 110
PleI GAGTC 2 cut(s) 471, 689
PpsI GAGTC 2 cut(s) 471, 689
PsiI TTATAA 1 cut(s) 353
Psp124BI GAGCTC 1 cut(s) 748
Psp6I CCWGG 1 cut(s) 428
PspGI CCWGG 1 cut(s) 428
PstI CTGCAG 1 cut(s) 575
RsaI GTAC 2 cut(s) 403, 664
RsaNI GTAC 2 cut(s) 402, 663
SacI GAGCTC 1 cut(s) 748
SaqAI TTAA 9 cut(s) 15, 20, 213, 225, 273, 300, 393, 609, 765
SatI GCNGC 1 cut(s) 109
Sau3AI GATC 2 cut(s) 561, 697
ScaI AGTACT 1 cut(s) 664
SchI GAGTC 2 cut(s) 472, 690
ScrFI CCNGG 1 cut(s) 430
SduI GDGCHC 1 cut(s) 748
SfaNI GCATC 1 cut(s) 42
SfcI CTRYAG 1 cut(s) 571
SmiI ATTTAAAT 1 cut(s) 21
SmlI CTYRAG 1 cut(s) 313
SmoI CTYRAG 1 cut(s) 313
SphI GCATGC 1 cut(s) 674
Sse9I AATT 6 cut(s) 16, 22, 76, 154, 244, 333
SspI AATATT 1 cut(s) 472
SspMI CTAG 3 cut(s) 750, 756, 784
SstI GAGCTC 1 cut(s) 748
StyD4I CCNGG 1 cut(s) 428
SwaI ATTTAAAT 1 cut(s) 21
TaaI ACNGT 2 cut(s) 223, 527
TaiI ACGT 1 cut(s) 631
TaqI TCGA 1 cut(s) 147
TasI AATT 6 cut(s) 16, 22, 76, 154, 244, 333
TatI WGTACW 1 cut(s) 662
TfiI GAWTC 3 cut(s) 362, 491, 497
Tru1I TTAA 9 cut(s) 15, 20, 213, 225, 273, 300, 393, 609, 765
Tru9I TTAA 9 cut(s) 15, 20, 213, 225, 273, 300, 393, 609, 765
TscAI CASTG 2 cut(s) 532, 781
TseFI GTSAC 2 cut(s) 527, 712
TseI GCWGC 1 cut(s) 108
Tsp45I GTSAC 2 cut(s) 527, 712
TspDTI ATGAA 6 cut(s) 20, 88, 257, 581, 608, 683
TspRI CASTG 2 cut(s) 532, 781
XapI RAATTY 2 cut(s) 76, 154
XceI RCATGY 2 cut(s) 617, 674
XmnI GAANNNNTTC 1 cut(s) 92
XspI CTAG 3 cut(s) 750, 756, 784
ZrmI AGTACT 1 cut(s) 664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.