Rmu_sc0001989.1_g000007

EF-hand domain pair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001989.1
OG0000254
Physical Location & Seq
Reverse (-)
17396 .. 18482
1087 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001989.1_g000007.1.cds

Sequence Viewer

Length: 570 bp
atgggagtggtgataattgacggaacgacggtgaaggacttcgtgagcgacgaggtgcagttcaagaagagcgtggacgagcgttttgcttctctggatctgaacaacgatggtgttctgtcccggtcggagctgcggaaagcattcgagtcgatgagattgatcgacacccacttcggcatagaagtggcgaccccgccggatcagctgacgcagctctacgattccatattcgataagttcgactgcgaccagagagggacggtggatctggacgacataaacttgggggttggccaaatcaccaatgctgaagctagggggatgctttctggattgaaacttgcttctgacctgaatatcagaaagcttgaagtagaatctgactcagctatctttgttaagttgctgattgatggttgtcctatatctcatcctttaggcaacattattgtagacatccaatcaatggttttgaagacgttaggatccgacatattttcagagaatgcaactatactgctgatactcttgctaagggaagtcttagccatgactatggtattgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7

Protein Analysis

189

Amino Acids

20.82

Weight (kDa)

4.43

Isoelectric Point (pI)

24.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000254)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25671 FvH4_1g29782 FvH4_2g06731 FvH4_2g09413 FvH4_2g12061 FvH4_2g12671 FvH4_2g14202 FvH4_3g27901 FvH4_3g30492 FvH4_3g36953 FvH4_4g10141 FvH4_4g11162 FvH4_5g27995 FvH4_5g34262 FvH4_6g10681 FvH4_6g13733 FvH4_6g18164 FvH4_6g25491 FvH4_6g25791 FvH4_6g27023 FvH4_6g34321 FvH4_7g05070
pyrus_communis pycom04g06850
rosa_chinensis RchiOBHm_Chr1g0322281 RchiOBHm_Chr1g0334981 RchiOBHm_Chr1g0371181 RchiOBHm_Chr2g0125461 RchiOBHm_Chr2g0127141 RchiOBHm_Chr2g0128961 RchiOBHm_Chr2g0132921 RchiOBHm_Chr3g0486471 RchiOBHm_Chr3g0492911 RchiOBHm_Chr4g0412131 RchiOBHm_Chr4g0426941 RchiOBHm_Chr4g0437331 RchiOBHm_Chr5g0018821 RchiOBHm_Chr5g0040021 RchiOBHm_Chr5g0051041 RchiOBHm_Chr6g0266871 RchiOBHm_Chr6g0271761 RchiOBHm_Chr7g0200931 RchiOBHm_Chr7g0220091
rosa_multiflora Rmu_co7991344.1_g000001 Rmu_sc0000172.1_g000008 Rmu_sc0000194.1_g000010 Rmu_sc0000446.1_g000026 Rmu_sc0000496.1_g000011 Rmu_sc0000530.1_g000013 Rmu_sc0000706.1_g000044 Rmu_sc0000745.1_g000029 Rmu_sc0001301.1_g000027 Rmu_sc0001520.1_g000008 Rmu_sc0001966.1_g000025 Rmu_sc0001989.1_g000007 Rmu_sc0002239.1_g000002 Rmu_sc0002816.1_g000004 Rmu_sc0003458.1_g000001 Rmu_sc0003825.1_g000031 Rmu_sc0003919.1_g000002 Rmu_sc0004053.1_g000007 Rmu_sc0004161.1_g000008 Rmu_sc0004368.1_g000033 Rmu_sc0004383.1_g000002 Rmu_sc0004923.1_g000002 Rmu_sc0005065.1_g000001 Rmu_sc0005478.1_g000001 Rmu_sc0006847.1_g000031 Rmu_sc0009556.1_g000002 Rmu_sc0010783.1_g000007 Rmu_sc0011424.1_g000022 Rmu_sc0013205.1_g000002 Rmu_sc0017407.1_g000001 Rmu_sc0023757.1_g000002 Rmu_sc0032346.1_g000001 Rmu_ssc0000008.1_g000014 Rmu_ssc0000158.1_g000033 Rmu_ssc0000217.1_g000028
rosa_roxburghii Rroxscaffold_1G00000480 Rroxscaffold_1G00026050 Rroxscaffold_1G00026250 Rroxscaffold_2G00085120 Rroxscaffold_2G00119710 Rroxscaffold_2G00126610 Rroxscaffold_2G00129340 Rroxscaffold_3G00245550 Rroxscaffold_5G00340210 Rroxscaffold_5G00340330 Rroxscaffold_6G00397180 Rroxscaffold_7G00216160
rosa_rugosa Rorug01G0040000 Rorug01G0067400 Rorug01G0092300 Rorug01G0197000 Rorug01G0474200 Rorug01G0482500 Rorug01G0489300 Rorug02G0142900 Rorug02G0259000 Rorug02G0283200 Rorug02G0341400 Rorug02G0341600 Rorug02G0370900 Rorug02G0379700 Rorug02G0386100 Rorug02G0387300 Rorug03G0112600 Rorug03G0265600 Rorug03G0298800 Rorug04G0019500 Rorug05G0069900 Rorug05G0070500 Rorug05G0256800 Rorug05G0287300 Rorug05G0592000 Rorug06G0057800 Rorug06G0082000 Rorug06G0118900 Rorug06G0176500 Rorug06G0423100 Rorug07G0050100 Rorug07G0095100 Rorug07G0202100 Rorug07G0216600 Rorug07G0244300 Rorug07G0245500
rosa_samantha Rh1DG154800 Rh2CG613000 Rh6BG016600 Rh6BG475100 Rh6CG013200 Rh7AG443800
rosa_wichuraiana Rw0G021840 Rw4G023650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 469
AccI GTMKAC 1 cut(s) 456
AciI CCGC 2 cut(s) 136, 197
AclWI GGATC 5 cut(s) 105, 210, 276, 483, 496
AcoI YGGCCR 1 cut(s) 295
AcuI CTGAAG 1 cut(s) 333
AfiI CCNNNNNNNGG 1 cut(s) 469
AgsI TTSAA 4 cut(s) 64, 340, 374, 478
AluBI AGCT 6 cut(s) 133, 208, 217, 317, 370, 392
AluI AGCT 6 cut(s) 133, 208, 217, 317, 370, 392
AlwI GGATC 5 cut(s) 105, 210, 276, 483, 496
AoxI GGCC 1 cut(s) 295
ApeKI GCWGC 2 cut(s) 133, 214
ArsI GACNNNNNNTTYG 4 cut(s) 68, 100, 158, 190
Asp700I GAANNNNTTC 2 cut(s) 38, 143
AsuC2I CCSGG 1 cut(s) 124
AsuHPI GGTGA 3 cut(s) 22, 43, 295
BalI TGGCCA 1 cut(s) 297
BamHI GGATCC 1 cut(s) 488
BbsI GAAGAC 1 cut(s) 485
BbvI GCAGC 2 cut(s) 120, 226
BccI CCATC 2 cut(s) 104, 410
BcgI CGANNNNNNTGC 4 cut(s) 68, 102, 132, 166
BcnI CCSGG 1 cut(s) 124
BfaI CTAG 1 cut(s) 318
BisI GCNGC 2 cut(s) 134, 215
BlsI GCNGC 2 cut(s) 135, 216
Bme1390I CCNGG 1 cut(s) 124
BmiI GGNNCC 1 cut(s) 490
BmrFI CCNGG 1 cut(s) 124
BmsI GCATC 1 cut(s) 315
BpiI GAAGAC 1 cut(s) 485
Bpu10I CCTNAGC 1 cut(s) 536
BpuMI CCSGG 1 cut(s) 124
Bsc4I CCNNNNNNNGG 1 cut(s) 469
BseGI GGATG 3 cut(s) 330, 433, 459
BseLI CCNNNNNNNGG 1 cut(s) 469
BseMII CTCAG 1 cut(s) 402
BseXI GCAGC 2 cut(s) 120, 226
BsgI GTGCAG 1 cut(s) 77
Bsh1285I CGRYCG 1 cut(s) 128
BshFI GGCC 1 cut(s) 297
BsiEI CGRYCG 1 cut(s) 128
BsiSI CCGG 2 cut(s) 124, 200
BslFI GGGAC 2 cut(s) 106, 274
BslI CCNNNNNNNGG 1 cut(s) 469
BsmFI GGGAC 2 cut(s) 106, 274
BsmI GAATGC 2 cut(s) 143, 514
BsnI GGCC 1 cut(s) 297
Bsp143I GATC 5 cut(s) 97, 162, 202, 268, 488
BspACI CCGC 2 cut(s) 136, 197
BspANI GGCC 1 cut(s) 297
BspCNI CTCAG 1 cut(s) 401
BspLI GGNNCC 1 cut(s) 490
BspPI GGATC 5 cut(s) 105, 210, 276, 483, 496
BspQI GCTCTTC 1 cut(s) 62
BssMI GATC 5 cut(s) 97, 162, 202, 268, 488
Bst4CI ACNGT 2 cut(s) 31, 265
Bst6I CTCTTC 1 cut(s) 62
BstDEI CTNAG 3 cut(s) 388, 536, 547
BstF5I GGATG 3 cut(s) 330, 433, 459
BstKTI GATC 5 cut(s) 100, 165, 205, 271, 491
BstMBI GATC 5 cut(s) 97, 162, 202, 268, 488
BstMCI CGRYCG 1 cut(s) 128
BstMWI GCNNNNNNNGC 2 cut(s) 205, 214
BstSCI CCNGG 1 cut(s) 122
BstV1I GCAGC 2 cut(s) 120, 226
BstV2I GAAGAC 1 cut(s) 485
BstX2I RGATCY 3 cut(s) 97, 268, 488
BstXI CCANNNNNNTGG 1 cut(s) 559
BstYI RGATCY 3 cut(s) 97, 268, 488
BsuRI GGCC 1 cut(s) 297
BtsCI GGATG 3 cut(s) 330, 433, 459
CseI GACGC 1 cut(s) 220
CviAII CATG 1 cut(s) 553
CviJI RGCY 8 cut(s) 133, 208, 217, 297, 317, 370, 392, 551
CviKI_1 RGCY 8 cut(s) 133, 208, 217, 297, 317, 370, 392, 551
DdeI CTNAG 3 cut(s) 388, 536, 547
DpnI GATC 5 cut(s) 99, 164, 204, 270, 490
DpnII GATC 5 cut(s) 97, 162, 202, 268, 488
EaeI YGGCCR 1 cut(s) 295
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
Eco57I CTGAAG 1 cut(s) 333
FaeI CATG 1 cut(s) 556
FaiI YATR 8 cut(s) 182, 230, 281, 428, 497, 518, 554, 560
FaqI GGGAC 2 cut(s) 106, 274
FatI CATG 1 cut(s) 552
FauI CCCGC 1 cut(s) 204
FblI GTMKAC 1 cut(s) 456
Fnu4HI GCNGC 2 cut(s) 134, 215
FokI GGATG 3 cut(s) 337, 420, 446
Fsp4HI GCNGC 2 cut(s) 134, 215
FspBI CTAG 1 cut(s) 318
GluI GCNGC 2 cut(s) 134, 215
HaeIII GGCC 1 cut(s) 297
HapII CCGG 2 cut(s) 124, 200
HgaI GACGC 1 cut(s) 220
Hin1II CATG 1 cut(s) 556
HindIII AAGCTT 1 cut(s) 368
HinfI GANTC 4 cut(s) 149, 224, 380, 386
HpaII CCGG 2 cut(s) 124, 200
HphI GGTGA 3 cut(s) 22, 43, 295
Hpy166II GTNNAC 2 cut(s) 76, 457
Hpy188I TCNGA 7 cut(s) 102, 130, 352, 365, 385, 493, 505
Hpy188III TCNNGA 5 cut(s) 43, 64, 95, 272, 333
Hpy8I GTNNAC 2 cut(s) 76, 457
Hpy99I CGWCG 2 cut(s) 31, 53
HpyAV CCTTC 1 cut(s) 28
HpyCH4III ACNGT 2 cut(s) 31, 265
HpyCH4IV ACGT 1 cut(s) 482
HpyCH4V TGCA 2 cut(s) 58, 512
HpyF10VI GCNNNNNNNGC 2 cut(s) 205, 214
HpyF3I CTNAG 3 cut(s) 388, 536, 547
HpySE526I ACGT 1 cut(s) 482
Hsp92II CATG 1 cut(s) 556
Kzo9I GATC 5 cut(s) 97, 162, 202, 268, 488
LguI GCTCTTC 1 cut(s) 62
LmnI GCTCC 1 cut(s) 130
LpnPI CCDG 7 cut(s) 80, 137, 213, 257, 266, 318, 368
Lsp1109I GCAGC 2 cut(s) 120, 226
LweI GCATC 1 cut(s) 315
MaeI CTAG 1 cut(s) 318
MaeII ACGT 1 cut(s) 482
MalI GATC 5 cut(s) 99, 164, 204, 270, 490
MboI GATC 5 cut(s) 97, 162, 202, 268, 488
MboII GAAGA 2 cut(s) 79, 490
MflI RGATCY 3 cut(s) 97, 268, 488
MlsI TGGCCA 1 cut(s) 297
MluCI AATT 1 cut(s) 15
MluNI TGGCCA 1 cut(s) 297
MlyI GAGTC 2 cut(s) 158, 380
MmeI TCCRAC 2 cut(s) 108, 516
MnlI CCTC 2 cut(s) 46, 251
Mox20I TGGCCA 1 cut(s) 297
MroXI GAANNNNTTC 2 cut(s) 38, 143
MscI TGGCCA 1 cut(s) 297
MseI TTAA 1 cut(s) 402
MslI CAYNNNNRTG 2 cut(s) 185, 557
Msp20I TGGCCA 1 cut(s) 297
MspA1I CMGCKG 1 cut(s) 208
MspI CCGG 2 cut(s) 124, 200
MspR9I CCNGG 1 cut(s) 124
Mva1269I GAATGC 2 cut(s) 143, 514
MwoI GCNNNNNNNGC 2 cut(s) 205, 214
NciI CCSGG 1 cut(s) 124
NdeII GATC 5 cut(s) 97, 162, 202, 268, 488
NlaIII CATG 1 cut(s) 556
NlaIV GGNNCC 1 cut(s) 490
PciSI GCTCTTC 1 cut(s) 62
PcsI WCGNNNNNNNCGW 2 cut(s) 48, 240
PctI GAATGC 2 cut(s) 143, 514
PdmI GAANNNNTTC 2 cut(s) 38, 143
PfeI GAWTC 2 cut(s) 224, 380
PflMI CCANNNNNTGG 1 cut(s) 469
PkrI GCNGC 2 cut(s) 135, 216
PleI GAGTC 2 cut(s) 157, 380
PpsI GAGTC 2 cut(s) 157, 380
PspN4I GGNNCC 1 cut(s) 490
PsuI RGATCY 3 cut(s) 97, 268, 488
PvuII CAGCTG 1 cut(s) 208
RseI CAYNNNNRTG 2 cut(s) 185, 557
SapI GCTCTTC 1 cut(s) 62
SaqAI TTAA 1 cut(s) 402
SatI GCNGC 2 cut(s) 134, 215
Sau3AI GATC 5 cut(s) 97, 162, 202, 268, 488
SchI GAGTC 2 cut(s) 158, 380
ScrFI CCNGG 1 cut(s) 124
SetI ASST 9 cut(s) 57, 135, 210, 219, 319, 357, 372, 394, 485
SfaNI GCATC 1 cut(s) 315
SmiMI CAYNNNNRTG 2 cut(s) 185, 557
Sse9I AATT 1 cut(s) 15
SsiI CCGC 2 cut(s) 136, 197
SspMI CTAG 1 cut(s) 318
StyD4I CCNGG 1 cut(s) 122
TaaI ACNGT 2 cut(s) 31, 265
TaiI ACGT 1 cut(s) 485
TaqI TCGA 5 cut(s) 147, 152, 165, 234, 243
TasI AATT 1 cut(s) 15
TfiI GAWTC 2 cut(s) 224, 380
Tru1I TTAA 1 cut(s) 402
Tru9I TTAA 1 cut(s) 402
TseI GCWGC 2 cut(s) 133, 214
TspGWI ACGGA 1 cut(s) 36
Van91I CCANNNNNTGG 1 cut(s) 469
XmiI GTMKAC 1 cut(s) 456
XmnI GAANNNNTTC 2 cut(s) 38, 143
XspI CTAG 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.