FvH4_5g34262

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000254
Physical Location & Seq
Forward (+)
24937173 .. 24937734
562 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g34262.t1

Sequence Viewer

Length: 516 bp
ATGCCCCCTCAAAATTATTACAAGCTCAATATTGATGGGACTAGAATTTCTAGCAGCAGTATGATTGGTGCTGGTGGTGTTATCAGAAACCATACTGGCGCTTGGATTGCAGGTTTTCAAATTAACCTTGGTGTTGGTGCTATCCTTGATGCTGAAGCTTGGGCGCTTTTTCATGGTTTGAAGTTGGCTTCCAAGCTTCATATCTCTTGCTTGGAAATTGAGTCTGACTCTGCAATTCTTGTTCAGCTTATGAACTGCTCCAATCTTTCCTCCCATCCCCTTGGTTCTTTACTTGGTGGCTGCAACAGCATGATGACTGAAATGGATGATGCGAAGCTGCTTCATATCTTCAGGGAGGCCAACATGACTGCAGATTCTTTGGCAAAATGCAGCATCTCTAATGAGCTTGGTCTTATTATTTTTGAGGATCCTCCTGTGCATGCCATTCCTGCCTACCTTGATGATCTAGCTGCTGTTACTAGAGCTAGGAGAACAAGCTCTTGTTTTAGTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

172

Amino Acids

18.35

Weight (kDa)

5.58

Isoelectric Point (pI)

44.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 10 - 129 1.5e-22 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000254)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25671 FvH4_1g29782 FvH4_2g06731 FvH4_2g09413 FvH4_2g12061 FvH4_2g12671 FvH4_2g14202 FvH4_3g27901 FvH4_3g30492 FvH4_3g36953 FvH4_4g10141 FvH4_4g11162 FvH4_5g27995 FvH4_5g34262 FvH4_6g10681 FvH4_6g13733 FvH4_6g18164 FvH4_6g25491 FvH4_6g25791 FvH4_6g27023 FvH4_6g34321 FvH4_7g05070
pyrus_communis pycom04g06850
rosa_chinensis RchiOBHm_Chr1g0322281 RchiOBHm_Chr1g0334981 RchiOBHm_Chr1g0371181 RchiOBHm_Chr2g0125461 RchiOBHm_Chr2g0127141 RchiOBHm_Chr2g0128961 RchiOBHm_Chr2g0132921 RchiOBHm_Chr3g0486471 RchiOBHm_Chr3g0492911 RchiOBHm_Chr4g0412131 RchiOBHm_Chr4g0426941 RchiOBHm_Chr4g0437331 RchiOBHm_Chr5g0018821 RchiOBHm_Chr5g0040021 RchiOBHm_Chr5g0051041 RchiOBHm_Chr6g0266871 RchiOBHm_Chr6g0271761 RchiOBHm_Chr7g0200931 RchiOBHm_Chr7g0220091
rosa_multiflora Rmu_co7991344.1_g000001 Rmu_sc0000172.1_g000008 Rmu_sc0000194.1_g000010 Rmu_sc0000446.1_g000026 Rmu_sc0000496.1_g000011 Rmu_sc0000530.1_g000013 Rmu_sc0000706.1_g000044 Rmu_sc0000745.1_g000029 Rmu_sc0001301.1_g000027 Rmu_sc0001520.1_g000008 Rmu_sc0001966.1_g000025 Rmu_sc0001989.1_g000007 Rmu_sc0002239.1_g000002 Rmu_sc0002816.1_g000004 Rmu_sc0003458.1_g000001 Rmu_sc0003825.1_g000031 Rmu_sc0003919.1_g000002 Rmu_sc0004053.1_g000007 Rmu_sc0004161.1_g000008 Rmu_sc0004368.1_g000033 Rmu_sc0004383.1_g000002 Rmu_sc0004923.1_g000002 Rmu_sc0005065.1_g000001 Rmu_sc0005478.1_g000001 Rmu_sc0006847.1_g000031 Rmu_sc0009556.1_g000002 Rmu_sc0010783.1_g000007 Rmu_sc0011424.1_g000022 Rmu_sc0013205.1_g000002 Rmu_sc0017407.1_g000001 Rmu_sc0023757.1_g000002 Rmu_sc0032346.1_g000001 Rmu_ssc0000008.1_g000014 Rmu_ssc0000158.1_g000033 Rmu_ssc0000217.1_g000028
rosa_roxburghii Rroxscaffold_1G00000480 Rroxscaffold_1G00026050 Rroxscaffold_1G00026250 Rroxscaffold_2G00085120 Rroxscaffold_2G00119710 Rroxscaffold_2G00126610 Rroxscaffold_2G00129340 Rroxscaffold_3G00245550 Rroxscaffold_5G00340210 Rroxscaffold_5G00340330 Rroxscaffold_6G00397180 Rroxscaffold_7G00216160
rosa_rugosa Rorug01G0040000 Rorug01G0067400 Rorug01G0092300 Rorug01G0197000 Rorug01G0474200 Rorug01G0482500 Rorug01G0489300 Rorug02G0142900 Rorug02G0259000 Rorug02G0283200 Rorug02G0341400 Rorug02G0341600 Rorug02G0370900 Rorug02G0379700 Rorug02G0386100 Rorug02G0387300 Rorug03G0112600 Rorug03G0265600 Rorug03G0298800 Rorug04G0019500 Rorug05G0069900 Rorug05G0070500 Rorug05G0256800 Rorug05G0287300 Rorug05G0592000 Rorug06G0057800 Rorug06G0082000 Rorug06G0118900 Rorug06G0176500 Rorug06G0423100 Rorug07G0050100 Rorug07G0095100 Rorug07G0202100 Rorug07G0216600 Rorug07G0244300 Rorug07G0245500
rosa_samantha Rh1DG154800 Rh2CG613000 Rh6BG016600 Rh6BG475100 Rh6CG013200 Rh7AG443800
rosa_wichuraiana Rw0G021840 Rw4G023650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 101
AclWI GGATC 2 cut(s) 422, 435
AcsI RAATTY 1 cut(s) 45
AcuI CTGAAG 2 cut(s) 174, 334
AgsI TTSAA 2 cut(s) 119, 181
AluBI AGCT 9 cut(s) 25, 158, 196, 247, 337, 406, 470, 485, 498
AluI AGCT 9 cut(s) 25, 158, 196, 247, 337, 406, 470, 485, 498
AlwI GGATC 2 cut(s) 422, 435
AoxI GGCC 1 cut(s) 357
ApeKI GCWGC 5 cut(s) 54, 300, 337, 390, 470
ApoI RAATTY 1 cut(s) 45
AspLEI GCGC 2 cut(s) 101, 166
BamHI GGATCC 1 cut(s) 427
BbvI GCAGC 5 cut(s) 66, 287, 324, 402, 457
BccI CCATC 2 cut(s) 29, 282
BfaI CTAG 5 cut(s) 42, 51, 467, 480, 486
BfmI CTRYAG 1 cut(s) 369
BfoI RGCGCY 2 cut(s) 102, 167
BfuAI ACCTGC 1 cut(s) 101
BisI GCNGC 5 cut(s) 55, 301, 338, 391, 471
BlsI GCNGC 5 cut(s) 56, 302, 339, 392, 472
BmiI GGNNCC 1 cut(s) 429
BmsI GCATC 3 cut(s) 139, 319, 402
BplI GAGNNNNNCTC 2 cut(s) 212, 244
BsaJI CCNNGG 2 cut(s) 127, 280
Bse1I ACTGG 1 cut(s) 100
BseDI CCNNGG 2 cut(s) 127, 280
BseGI GGATG 2 cut(s) 274, 331
BseNI ACTGG 1 cut(s) 100
BseXI GCAGC 5 cut(s) 66, 287, 324, 402, 457
BshFI GGCC 1 cut(s) 359
BslFI GGGAC 1 cut(s) 52
BsmFI GGGAC 1 cut(s) 52
BsnI GGCC 1 cut(s) 359
Bsp143I GATC 2 cut(s) 427, 463
BspANI GGCC 1 cut(s) 359
BspLI GGNNCC 1 cut(s) 429
BspMAI CTGCAG 1 cut(s) 373
BspMI ACCTGC 1 cut(s) 101
BspPI GGATC 2 cut(s) 422, 435
BsrI ACTGG 1 cut(s) 100
BssECI CCNNGG 2 cut(s) 127, 280
BssMI GATC 2 cut(s) 427, 463
BssT1I CCWWGG 2 cut(s) 127, 280
BstC8I GCNNGC 1 cut(s) 441
BstF5I GGATG 2 cut(s) 274, 331
BstH2I RGCGCY 2 cut(s) 102, 167
BstHHI GCGC 2 cut(s) 101, 166
BstKTI GATC 2 cut(s) 430, 466
BstMBI GATC 2 cut(s) 427, 463
BstMWI GCNNNNNNNGC 3 cut(s) 107, 306, 449
BstNSI RCATGY 1 cut(s) 443
BstSFI CTRYAG 1 cut(s) 369
BstV1I GCAGC 5 cut(s) 66, 287, 324, 402, 457
BstX2I RGATCY 1 cut(s) 427
BstXI CCANNNNNNTGG 1 cut(s) 281
BstYI RGATCY 1 cut(s) 427
BsuRI GGCC 1 cut(s) 359
BtsCI GGATG 2 cut(s) 274, 331
BveI ACCTGC 1 cut(s) 101
Cac8I GCNNGC 1 cut(s) 441
CfoI GCGC 2 cut(s) 101, 166
CviAII CATG 4 cut(s) 173, 310, 364, 440
DpnI GATC 2 cut(s) 429, 465
DpnII GATC 2 cut(s) 427, 463
Eco130I CCWWGG 2 cut(s) 127, 280
Eco57I CTGAAG 2 cut(s) 174, 334
EcoT14I CCWWGG 2 cut(s) 127, 280
ErhI CCWWGG 2 cut(s) 127, 280
FaeI CATG 4 cut(s) 176, 313, 367, 443
FaiI YATR 9 cut(s) 62, 93, 174, 201, 251, 311, 345, 365, 441
FaqI GGGAC 1 cut(s) 52
FatI CATG 4 cut(s) 172, 309, 363, 439
Fnu4HI GCNGC 5 cut(s) 55, 301, 338, 391, 471
FokI GGATG 2 cut(s) 261, 338
Fsp4HI GCNGC 5 cut(s) 55, 301, 338, 391, 471
FspBI CTAG 5 cut(s) 42, 51, 467, 480, 486
GlaI GCGC 2 cut(s) 100, 165
GluI GCNGC 5 cut(s) 55, 301, 338, 391, 471
HaeII RGCGCY 2 cut(s) 102, 167
HaeIII GGCC 1 cut(s) 359
HhaI GCGC 2 cut(s) 101, 166
Hin1II CATG 4 cut(s) 176, 313, 367, 443
Hin6I GCGC 2 cut(s) 99, 164
HinP1I GCGC 2 cut(s) 99, 164
HindIII AAGCTT 2 cut(s) 156, 194
HinfI GANTC 3 cut(s) 221, 227, 374
Hpy188I TCNGA 2 cut(s) 86, 226
HpyCH4V TGCA 6 cut(s) 110, 233, 303, 371, 390, 439
HpyF10VI GCNNNNNNNGC 3 cut(s) 107, 306, 449
Hsp92II CATG 4 cut(s) 176, 313, 367, 443
HspAI GCGC 2 cut(s) 99, 164
Kzo9I GATC 2 cut(s) 427, 463
LmnI GCTCC 1 cut(s) 263
LpnPI CCDG 6 cut(s) 57, 81, 96, 337, 447, 462
Lsp1109I GCAGC 5 cut(s) 66, 287, 324, 402, 457
LweI GCATC 3 cut(s) 139, 319, 402
MaeI CTAG 5 cut(s) 42, 51, 467, 480, 486
MaeIII GTNAC 1 cut(s) 475
MalI GATC 2 cut(s) 429, 465
MboI GATC 2 cut(s) 427, 463
MboII GAAGA 1 cut(s) 340
MflI RGATCY 1 cut(s) 427
MluCI AATT 5 cut(s) 13, 45, 120, 216, 234
MlyI GAGTC 2 cut(s) 221, 230
MnlI CCTC 5 cut(s) 18, 280, 349, 418, 441
MseI TTAA 1 cut(s) 123
MwoI GCNNNNNNNGC 3 cut(s) 107, 306, 449
NdeII GATC 2 cut(s) 427, 463
NlaIII CATG 4 cut(s) 176, 313, 367, 443
NlaIV GGNNCC 1 cut(s) 429
NspI RCATGY 1 cut(s) 443
PaeI GCATGC 1 cut(s) 443
PfeI GAWTC 1 cut(s) 374
PkrI GCNGC 5 cut(s) 56, 302, 339, 392, 472
PleI GAGTC 2 cut(s) 221, 229
PpsI GAGTC 2 cut(s) 221, 229
PspN4I GGNNCC 1 cut(s) 429
PstI CTGCAG 1 cut(s) 373
PsuI RGATCY 1 cut(s) 427
SaqAI TTAA 1 cut(s) 123
SatI GCNGC 5 cut(s) 55, 301, 338, 391, 471
Sau3AI GATC 2 cut(s) 427, 463
SchI GAGTC 2 cut(s) 221, 230
SfaNI GCATC 3 cut(s) 139, 319, 402
SfcI CTRYAG 1 cut(s) 369
SphI GCATGC 1 cut(s) 443
Sse9I AATT 5 cut(s) 13, 45, 120, 216, 234
SspI AATATT 1 cut(s) 31
SspMI CTAG 5 cut(s) 42, 51, 467, 480, 486
StyI CCWWGG 2 cut(s) 127, 280
TasI AATT 5 cut(s) 13, 45, 120, 216, 234
TfiI GAWTC 1 cut(s) 374
Tru1I TTAA 1 cut(s) 123
Tru9I TTAA 1 cut(s) 123
TseI GCWGC 5 cut(s) 54, 300, 337, 390, 470
TspDTI ATGAA 4 cut(s) 161, 188, 266, 332
XapI RAATTY 1 cut(s) 45
XceI RCATGY 1 cut(s) 443
XspI CTAG 5 cut(s) 42, 51, 467, 480, 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.