Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0015587

Physical Location & Seq

Genomic Coordinates Reverse (-)

83573278 .. 83575622

Gene Structure 2345 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG490400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1536 bp

ATGGATATGGAGCACCAAGAGATCACTAACCCTGAGATCACTGAGAGCAACAACAATGGAGGAGGACTACTGATCAAGCTCAAGATGGCAAAGGCTGAACCTTTTTCGGAACAAGGTAATCGGAAGCCCGAAAACCTTGCTCCTCAATTCACTAAAATCTGTCATATTTGCCACAAAGGTTTCGAGTCCGGGAAAGCTCTGGGAGGTCACATGAGAATGCATGTCCAAGAGAGCAAGGGACTGATCAAGAGCTCTTCGAAATCTCCGAAGCCGGTGGTCGTGAAAAATAGTGCCACTGAATTCAGTACTAGTACGACTGATTCCAGTGGTGAACCTGTCTGCATAGAGTGTGGTAAGATTTTCCCGTCAAAGAAATCTCTCTATGGACACATGAGGTCACACCCGGATAGGCCTTATAGGGGAATTGAGCCTCCTCAATCAACCAAGAACAGCTCCACTTCAACTTTATCTGATGCTTTGCCTCAAAATAATCACAAGGCAGCTGAGGATCAGCAGATTGATTCTGCTGGGACAGCTCGAATCTGGTTCGGGCCTAATCCGAAAGTGGTTCCTGATCTGGTTAAGATTGTGCCTAGCTGGTCTCAAACTGCGAAAAGAGGTACGAAAGGTACTAGTCTTTCTGAAACCAACTCTGAGACTGATGATGATTGTGATGATGATGATGATGATGATGATGAGTTGGATTTGAGTGAAGCTGTCCATGATCTGATGATGTTAGCTCAAGCCAATCCAAAAGGAGCTGAGGAGGCTACCAACAGCAATTTCATGACCAAAAACGAGGATCGTGATGGAAAGGACAGAACGGTAATTGACTATGTGGGTGTAGCCAAGAAGAGGAAGCAGAAGCTTGAAATTGAGGAGGATGGGGATTTTGTAAGTGGGTTTGATCTAGGGAAGTCTAGGAATCACTTGGAGCTGGGAGTGGATTTGAAAGAGGACAGTGAAGAGGAGTTATCGGATAGTGAAAACTCTGAGAGCATTGTGCTTGCAAACATGATGAGGAGGAAGAAGAGAAGGAAGATGAAATTGGAAGACTTGGAAGGAGCGGTTGTTGCTCAAGATCATCAACATCAAAGCCATCATCACAAAGTTTATCCTTGCAGTCTTTGCAGCAAGTCATTCCAAAGCCACCAAGCATTGGGAGGACACATGTCAAGCCACAACAAGTTCAAGAACAACATCCAGTACCCTGTGGAACATGATCATCATCAATCTGGTTCTGACGATACTAATAATGGAAATGCTAATCTCACCAACGCAGCCACCCAAGTTGACCATGATCAGGCTGAAAGTCACCAATGCAGATTCTGTAACAGGACTTTTCCAACAGGTCAAGCATTGGGAGGTCATATGAGGTCTCACTGGACCGGTCCAAATGATCCAGCTCAATCGAGCCAGACCGGGCGCAAAGGCCTCGACATTGACCTCAATGAGCTTCCACCTATGGAGTATGAACAAGGTATAGACTACTCTGGAGCTGGTTATGCAACTTCTTCTTATAATTCAGTTACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0007276 GO:0008150 GO:0009555 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0019953 GO:0022412 GO:0022414 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032504 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0044703 GO:0048229 GO:0048232 GO:0048235 GO:0048609 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051704 GO:0055046 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-C2H2_6

Protein Analysis

511

Amino Acids

56.44

Weight (kDa)

5.61

Isoelectric Point (pI)

49.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-C2H2_6	PF13912	53 - 74	2.6e-07	C2H2-type zinc finger
zf-C2H2_6	PF13912	371 - 395	1.6e-09	C2H2-type zinc finger
zf-C2H2_6	PF13912	439 - 462	5.8e-09	C2H2-type zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015587)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g41600
malus_domestica	MD03G1043000.v1.1 MD11G1046000.v1.1
prunus_persica	Prupe.6G035400_v2.0.a1
pyrus_communis	pycom03g03320 pycom11g03730
rosa_chinensis	RchiOBHm_Chr5g0074621
rosa_laevigata	RLG00000036491
rosa_multiflora	Rmu_sc0001104.1_g000006 Rmu_sc0019989.1_g000001
rosa_roxburghii	Rroxscaffold_1G00006960
rosa_rugosa	Rorug05G0433300
rosa_samantha	Rh5AG490400 Rh5BG511300 Rh5CG535000 Rh5DG523800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1521
AccB7I	CCANNNNNTGG	1 cut(s)	1159
AccBSI	CCGCTC	1 cut(s)	1067
AciI	CCGC	1 cut(s)	1067
AclWI	GGATC	3 cut(s)	516, 810, 1394
AcsI	RAATTY	1 cut(s)	299
AfaI	GTAC	5 cut(s)	307, 313, 622, 631, 1208
AfiI	CCNNNNNNNGG	4 cut(s)	419, 855, 1159, 1303
AflIII	ACRYGT	1 cut(s)	1170
AgeI	ACCGGT	1 cut(s)	1388
AgsI	TTSAA	4 cut(s)	462, 872, 952, 1192
AhlI	ACTAGT	2 cut(s)	308, 632
AjuI	GAANNNNNNNTTGG	4 cut(s)	767, 799, 1031, 1063
Alw21I	GWGCWC	2 cut(s)	15, 254
Alw26I	GTCTC	3 cut(s)	606, 650, 1383
AlwI	GGATC	3 cut(s)	516, 810, 1394
AlwNI	CAGNNNCTG	1 cut(s)	1329
AoxI	GGCC	3 cut(s)	410, 551, 1432
ApeKI	GCWGC	3 cut(s)	500, 1131, 1280
ApoI	RAATTY	1 cut(s)	299
AsiGI	ACCGGT	1 cut(s)	1388
AspLEI	GCGC	1 cut(s)	1428
AspS9I	GGNCC	3 cut(s)	551, 1386, 1391
AsuC2I	CCSGG	3 cut(s)	190, 404, 1423
AsuHPI	GGTGA	3 cut(s)	341, 1264, 1307
AsuII	TTCGAA	1 cut(s)	257
AvaII	GGWCC	2 cut(s)	1386, 1391
BanII	GRGCYC	1 cut(s)	254
BbsI	GAAGAC	1 cut(s)	1059
Bbv12I	GWGCWC	2 cut(s)	15, 254
BbvCI	CCTCAGC	2 cut(s)	504, 762
BbvI	GCAGC	3 cut(s)	512, 1143, 1292
BccI	CCATC	4 cut(s)	79, 803, 878, 1107
BcgI	CGANNNNNNTGC	4 cut(s)	119, 153, 1417, 1451
BclI	TGATCA	4 cut(s)	72, 243, 1222, 1300
BcnI	CCSGG	3 cut(s)	190, 404, 1423
BcoDI	GTCTC	3 cut(s)	606, 650, 1383
BcuI	ACTAGT	2 cut(s)	308, 632
BfaI	CTAG	5 cut(s)	309, 594, 633, 911, 921
BisI	GCNGC	3 cut(s)	501, 1132, 1281
BlsI	GCNGC	3 cut(s)	502, 1133, 1282
BmcAI	AGTACT	1 cut(s)	307
Bme1390I	CCNGG	3 cut(s)	190, 404, 1423
Bme18I	GGWCC	2 cut(s)	1386, 1391
BmgT120I	GGNCC	3 cut(s)	551, 1386, 1391
BmiI	GGNNCC	1 cut(s)	570
BmrFI	CCNGG	3 cut(s)	190, 404, 1423
BmsI	GCATC	1 cut(s)	463
BoxI	GACNNNNGTC	1 cut(s)	1171
BpiI	GAAGAC	1 cut(s)	1059
BpmI	CTGGAG	1 cut(s)	1515
Bpu10I	CCTNAGC	2 cut(s)	504, 762
Bpu14I	TTCGAA	1 cut(s)	257
BpuEI	CTTGAG	3 cut(s)	65, 726, 1062
BpuMI	CCSGG	3 cut(s)	190, 404, 1423
BsaBI	GATNNNNATC	1 cut(s)	1227
BsaI	GGTCTC	2 cut(s)	606, 1383
BsaWI	WCCGGW	1 cut(s)	1388
BsaXI	ACNNNNNCTCC	2 cut(s)	1056, 1086
Bsc4I	CCNNNNNNNGG	4 cut(s)	419, 855, 1159, 1303
Bse118I	RCCGGY	2 cut(s)	271, 1388
Bse1I	ACTGG	3 cut(s)	324, 1204, 1388
Bse8I	GATNNNNATC	1 cut(s)	1227
BseGI	GGATG	2 cut(s)	889, 1200
BseJI	GATNNNNATC	1 cut(s)	1227
BseLI	CCNNNNNNNGG	4 cut(s)	419, 855, 1159, 1303
BseMII	CTCAG	6 cut(s)	24, 33, 495, 645, 753, 984
BseNI	ACTGG	3 cut(s)	324, 1204, 1388
BseRI	GAGGAG	7 cut(s)	75, 132, 423, 779, 893, 983, 1036
BseXI	GCAGC	3 cut(s)	512, 1143, 1292
BseYI	CCCAGC	2 cut(s)	527, 937
BshFI	GGCC	3 cut(s)	412, 553, 1434
BshTI	ACCGGT	1 cut(s)	1388
BsiHKAI	GWGCWC	2 cut(s)	15, 254
BsiSI	CCGG	5 cut(s)	189, 272, 404, 1389, 1422
BslFI	GGGAC	2 cut(s)	252, 544
BslI	CCNNNNNNNGG	4 cut(s)	419, 855, 1159, 1303
BsmAI	GTCTC	3 cut(s)	606, 650, 1383
BsmFI	GGGAC	2 cut(s)	252, 544
BsmI	GAATGC	1 cut(s)	222
BsnI	GGCC	3 cut(s)	412, 553, 1434
Bso31I	GGTCTC	2 cut(s)	606, 1383
Bsp119I	TTCGAA	1 cut(s)	257
Bsp1286I	GDGCHC	2 cut(s)	15, 254
BspACI	CCGC	1 cut(s)	1067
BspANI	GGCC	3 cut(s)	412, 553, 1434
BspCNI	CTCAG	6 cut(s)	25, 34, 496, 646, 754, 985
BspHI	TCATGA	1 cut(s)	786
BspLI	GGNNCC	1 cut(s)	570
BspPI	GGATC	3 cut(s)	516, 810, 1394
BspQI	GCTCTTC	1 cut(s)	259
BspT104I	TTCGAA	1 cut(s)	257
BspTNI	GGTCTC	2 cut(s)	606, 1383
BsrBI	CCGCTC	1 cut(s)	1067
BsrFI	RCCGGY	2 cut(s)	271, 1388
BsrI	ACTGG	3 cut(s)	324, 1204, 1388
BssAI	RCCGGY	2 cut(s)	271, 1388
Bst4CI	ACNGT	2 cut(s)	826, 962
Bst6I	CTCTTC	4 cut(s)	259, 848, 960, 1025
BstAPI	GCANNNNNTGC	1 cut(s)	1128
BstBI	TTCGAA	1 cut(s)	257
BstC8I	GCNNGC	1 cut(s)	1008
BstDEI	CTNAG	6 cut(s)	33, 42, 504, 654, 762, 993
BstF5I	GGATG	2 cut(s)	889, 1200
BstHHI	GCGC	1 cut(s)	1428
BstMAI	GTCTC	3 cut(s)	606, 650, 1383
BstMWI	GCNNNNNNNGC	5 cut(s)	533, 767, 1073, 1128, 1505
BstNSI	RCATGY	2 cut(s)	224, 1174
BstPAI	GACNNNNGTC	1 cut(s)	1171
BstSCI	CCNGG	3 cut(s)	188, 402, 1421
BstV1I	GCAGC	3 cut(s)	512, 1143, 1292
BstV2I	GAAGAC	1 cut(s)	1059
BsuRI	GGCC	3 cut(s)	412, 553, 1434
BtsCI	GGATG	2 cut(s)	889, 1200
BtsIMutI	CAGTG	5 cut(s)	39, 294, 331, 967, 1381
Cac8I	GCNNGC	1 cut(s)	1008
CaiI	CAGNNNCTG	1 cut(s)	1329
CciI	TCATGA	1 cut(s)	786
CfoI	GCGC	1 cut(s)	1428
Cfr10I	RCCGGY	2 cut(s)	271, 1388
Cfr13I	GGNCC	3 cut(s)	551, 1386, 1391
Csp6I	GTAC	5 cut(s)	306, 312, 621, 630, 1207
CspAI	ACCGGT	1 cut(s)	1388
CspCI	CAANNNNNGTGG	2 cut(s)	1273, 1308
CviAII	CATG	9 cut(s)	211, 221, 391, 722, 787, 1015, 1171, 1220, 1298
CviQI	GTAC	5 cut(s)	306, 312, 621, 630, 1207
DdeI	CTNAG	6 cut(s)	33, 42, 504, 654, 762, 993
Eam1104I	CTCTTC	4 cut(s)	259, 848, 960, 1025
EarI	CTCTTC	4 cut(s)	259, 848, 960, 1025
Ecl136II	GAGCTC	1 cut(s)	252
Eco147I	AGGCCT	2 cut(s)	412, 1434
Eco24I	GRGCYC	1 cut(s)	254
Eco31I	GGTCTC	2 cut(s)	606, 1383
Eco47I	GGWCC	2 cut(s)	1386, 1391
Eco53kI	GAGCTC	1 cut(s)	252
EcoICRI	GAGCTC	1 cut(s)	252
EcoRI	GAATTC	1 cut(s)	299
EcoT22I	ATGCAT	1 cut(s)	222
EcoT38I	GRGCYC	1 cut(s)	254
FaeI	CATG	9 cut(s)	214, 224, 394, 725, 790, 1018, 1174, 1223, 1301
FaqI	GGGAC	2 cut(s)	252, 544
FatI	CATG	9 cut(s)	210, 220, 390, 721, 786, 1014, 1170, 1219, 1297
FauNDI	CATATG	1 cut(s)	1371
FbaI	TGATCA	4 cut(s)	72, 243, 1222, 1300
Fnu4HI	GCNGC	3 cut(s)	501, 1132, 1281
FokI	GGATG	2 cut(s)	896, 1187
FriOI	GRGCYC	1 cut(s)	254
Fsp4HI	GCNGC	3 cut(s)	501, 1132, 1281
FspBI	CTAG	5 cut(s)	309, 594, 633, 911, 921
GlaI	GCGC	1 cut(s)	1427
GluI	GCNGC	3 cut(s)	501, 1132, 1281
GsaI	CCCAGC	2 cut(s)	531, 941
GsuI	CTGGAG	1 cut(s)	1515
HaeIII	GGCC	3 cut(s)	412, 553, 1434
HapII	CCGG	5 cut(s)	189, 272, 404, 1389, 1422
HhaI	GCGC	1 cut(s)	1428
Hin1II	CATG	9 cut(s)	214, 224, 394, 725, 790, 1018, 1174, 1223, 1301
Hin6I	GCGC	1 cut(s)	1426
HinP1I	GCGC	1 cut(s)	1426
HincII	GTYRAC	1 cut(s)	1294
HindII	GTYRAC	1 cut(s)	1294
HindIII	AAGCTT	1 cut(s)	866
HinfI	GANTC	6 cut(s)	185, 320, 521, 540, 925, 1326
HpaII	CCGG	5 cut(s)	189, 272, 404, 1389, 1422
HphI	GGTGA	3 cut(s)	341, 1264, 1307
Hpy166II	GTNNAC	2 cut(s)	332, 1294
Hpy188III	TCNNGA	9 cut(s)	82, 247, 280, 572, 787, 806, 1079, 1192, 1494
Hpy8I	GTNNAC	2 cut(s)	332, 1294
HpyAV	CCTTC	2 cut(s)	1029, 1055
HpyCH4III	ACNGT	2 cut(s)	826, 962
HpyCH4V	TGCA	7 cut(s)	220, 342, 1010, 1122, 1131, 1323, 1508
HpyF10VI	GCNNNNNNNGC	5 cut(s)	533, 767, 1073, 1128, 1505
HpyF3I	CTNAG	6 cut(s)	33, 42, 504, 654, 762, 993
Hsp92II	CATG	9 cut(s)	214, 224, 394, 725, 790, 1018, 1174, 1223, 1301
HspAI	GCGC	1 cut(s)	1426
Ksp22I	TGATCA	4 cut(s)	72, 243, 1222, 1300
LguI	GCTCTTC	1 cut(s)	259
LmnI	GCTCC	7 cut(s)	10, 145, 458, 758, 934, 1064, 1496
Lsp1109I	GCAGC	3 cut(s)	512, 1143, 1292
LweI	GCATC	1 cut(s)	463
MaeI	CTAG	5 cut(s)	309, 594, 633, 911, 921
MaeIII	GTNAC	5 cut(s)	206, 396, 1313, 1331, 1528
MbiI	CCGCTC	1 cut(s)	1067
MboII	GAAGA	8 cut(s)	246, 865, 977, 1039, 1042, 1051, 1064, 1506
MhlI	GDGCHC	2 cut(s)	15, 254
MluCI	AATT	8 cut(s)	146, 299, 423, 781, 828, 873, 1046, 1522
MlyI	GAGTC	1 cut(s)	194
MmeI	TCCRAC	2 cut(s)	681, 1370
Mph1103I	ATGCAT	1 cut(s)	222
MseI	TTAA	2 cut(s)	582, 1534
MslI	CAYNNNNRTG	1 cut(s)	215
MspA1I	CMGCKG	1 cut(s)	503
MspI	CCGG	5 cut(s)	189, 272, 404, 1389, 1422
MspR9I	CCNGG	3 cut(s)	190, 404, 1423
Mva1269I	GAATGC	1 cut(s)	222
MwoI	GCNNNNNNNGC	5 cut(s)	533, 767, 1073, 1128, 1505
NciI	CCSGG	3 cut(s)	190, 404, 1423
NdeI	CATATG	1 cut(s)	1371
NlaIII	CATG	9 cut(s)	214, 224, 394, 725, 790, 1018, 1174, 1223, 1301
NlaIV	GGNNCC	1 cut(s)	570
NmuCI	GTSAC	3 cut(s)	206, 396, 1313
NsiI	ATGCAT	1 cut(s)	222
NspI	RCATGY	2 cut(s)	224, 1174
NspV	TTCGAA	1 cut(s)	257
PagI	TCATGA	1 cut(s)	786
PceI	AGGCCT	2 cut(s)	412, 1434
PciI	ACATGT	1 cut(s)	1170
PciSI	GCTCTTC	1 cut(s)	259
PcsI	WCGNNNNNNNCGW	1 cut(s)	263
PctI	GAATGC	1 cut(s)	222
PfeI	GAWTC	5 cut(s)	320, 521, 540, 925, 1326
PflMI	CCANNNNNTGG	1 cut(s)	1159
PfoI	TCCNGGA	1 cut(s)	188
PinAI	ACCGGT	1 cut(s)	1388
PkrI	GCNGC	3 cut(s)	502, 1133, 1282
PleI	GAGTC	1 cut(s)	193
PpsI	GAGTC	1 cut(s)	193
PscI	ACATGT	1 cut(s)	1170
PshAI	GACNNNNGTC	1 cut(s)	1171
PsiI	TTATAA	1 cut(s)	1521
Psp124BI	GAGCTC	1 cut(s)	254
PspFI	CCCAGC	2 cut(s)	527, 937
PspN4I	GGNNCC	1 cut(s)	570
PspPI	GGNCC	3 cut(s)	551, 1386, 1391
PstNI	CAGNNNCTG	1 cut(s)	1329
PvuII	CAGCTG	1 cut(s)	503
RsaI	GTAC	5 cut(s)	307, 313, 622, 631, 1208
RsaNI	GTAC	5 cut(s)	306, 312, 621, 630, 1207
RseI	CAYNNNNRTG	1 cut(s)	215
SacI	GAGCTC	1 cut(s)	254
SapI	GCTCTTC	1 cut(s)	259
SaqAI	TTAA	2 cut(s)	582, 1534
SatI	GCNGC	3 cut(s)	501, 1132, 1281
Sau96I	GGNCC	3 cut(s)	551, 1386, 1391
ScaI	AGTACT	1 cut(s)	307
SchI	GAGTC	1 cut(s)	194
ScrFI	CCNGG	3 cut(s)	190, 404, 1423
SduI	GDGCHC	2 cut(s)	15, 254
SfaNI	GCATC	1 cut(s)	463
SfuI	TTCGAA	1 cut(s)	257
SinI	GGWCC	2 cut(s)	1386, 1391
SmiMI	CAYNNNNRTG	1 cut(s)	215
SmlI	CTYRAG	3 cut(s)	80, 741, 1077
SmoI	CTYRAG	3 cut(s)	80, 741, 1077
SpeI	ACTAGT	2 cut(s)	308, 632
Sse9I	AATT	8 cut(s)	146, 299, 423, 781, 828, 873, 1046, 1522
SseBI	AGGCCT	2 cut(s)	412, 1434
SsiI	CCGC	1 cut(s)	1067
SspMI	CTAG	5 cut(s)	309, 594, 633, 911, 921
SstI	GAGCTC	1 cut(s)	254
StuI	AGGCCT	2 cut(s)	412, 1434
StyD4I	CCNGG	3 cut(s)	188, 402, 1421
TaaI	ACNGT	2 cut(s)	826, 962
TaqI	TCGA	5 cut(s)	183, 257, 538, 1412, 1437
TasI	AATT	8 cut(s)	146, 299, 423, 781, 828, 873, 1046, 1522
TatI	WGTACW	1 cut(s)	305
TfiI	GAWTC	5 cut(s)	320, 521, 540, 925, 1326
Tru1I	TTAA	2 cut(s)	582, 1534
Tru9I	TTAA	2 cut(s)	582, 1534
TscAI	CASTG	5 cut(s)	46, 301, 331, 967, 1388
TseFI	GTSAC	3 cut(s)	206, 396, 1313
TseI	GCWGC	3 cut(s)	500, 1131, 1280
Tsp45I	GTSAC	3 cut(s)	206, 396, 1313
TspDTI	ATGAA	3 cut(s)	775, 1058, 1488
TspRI	CASTG	5 cut(s)	46, 301, 331, 967, 1388
Van91I	CCANNNNNTGG	1 cut(s)	1159
VpaK11BI	GGWCC	2 cut(s)	1386, 1391
XapI	RAATTY	1 cut(s)	299
XceI	RCATGY	2 cut(s)	224, 1174
XspI	CTAG	5 cut(s)	309, 594, 633, 911, 921
ZrmI	AGTACT	1 cut(s)	307
Zsp2I	ATGCAT	1 cut(s)	222

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.