Rh5BG267600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0006669
Physical Location & Seq
Forward (+)
33458747 .. 33462569
3823 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG267600.1

Sequence Viewer

Length: 1569 bp
ATGGCTTCCCCCTCTCCCTCTTTACTCCTCGCAGCAGCCATCACCCTCACTCTGCTCTTCTCATACTCGGTCAAATTACCATTCCACCCCCGCGACCTACTCCCTCTCCTTCCCAGACAGCTCTCATGGCCAATCCTCAACTCCCTCCACAGCGCAGTCGATCTCCTCCCCACTTTCGTCGGCTCCGCTTCGTCCCAAAACAACACTCTCGGCTGGAAAGGCGCCTGCTTTTACCAGAACACGGCTTGGATGGAGTTCCACAACAAAACCGGCTCACAATTTGGTGGCGGAACTCTTCATATCAAGGTTAGCAAGGCTCATAGTTGGACGTGTATGGATATTTATGTCTTTGCAACTCCATATACTGTCACATGGGATTACTATCTTCTGTCGCGGGAGCACACACTCGAGTTTAAGGAGTGGACAGGGAAAGCTGAGTATGAATTTGTTAAAAACAGGGGAGTTTCTATATTCCTCATGCAAGCGGGGATGTTAGGCACCCTTCAAGCATTGTGGGATGTCTTTCCCTTGTTTACAAATACAGGATGGGGAGAGCTGTCCAATATTGGGTTTCTCAAAAAGCATATGGGTGCAACCTTTGAGCAACGTCCTCAACCTTGGGTAACAAATATTAGCACTGATGATGTTCACTCCGGAGATTTCCTTGCAATATCTAAGATTCGCGGGAGGTGGGGTGGATTTGAGACTTTAGAGAAGTGGGTCAGTGGAGCTTACGCTGGCCATTCTGCAGTTTGTTTAAGGGATTCTGAAGGAAAGTTGTGGGTTGGTGAATCAGGAAATGAAAATGAGCAGGGAGAAGATATTATTGCTATTCTGCCATGGGATGAGTGGTGGGATTTTGAGCTAAACAAGGATGATTCCGATCCCCACATTGCATTGCTTCCCTTGCATCCTGATATCCGAGCGAAGTTTAACGAGACTGCGGCCTGGGAGTATGCAAGGAGCATGGAAGGCCAACCATATGGTTATCATAACATGATATTCAGCTGGATAGATACTATAGATGAAAACTATCCACCCCCTTTGGATGCTCATGTGATTGCTTCTGTTATGACAGTTTGGAATCAAATAAAGCCTACATATGCTGCTAACTTGTGGAATGAAGCCTTGAACAAGCGACTTGAAACTCAGAACCTTAGCTTTCCTGAGATCTTGGTTGAAGTTGAAAAGCGTGGGTCATCGTTTGATCAATTGCTTACAATTCCGGAACAAGATGATTGGCTGTACAGTGATGGGAAGTCAACCTCGTGCGTTGCCTTCATTCTTGAAATGTATAAGGAGGCTGGACTTTTTGATCCAATTGCAAGCTCTATCCAAGTTACTGAATTTACGATAAAAGATGCTTACATGCTCAAGTTCTTTGAAAATGATTCGAGCCGCCTGCCCAAGTGGTGCAATGATGGGGACACCGTGAAGCTTCCGTTCTGTCAGATTAAAGGGAAGTATCGAATGGAATTGCCTGAATACAACATCATGGAACCATACCCCCATATGAATGAGAGATGCCCATCACTGCCCCCCAAGTATTCCAGGCGGCAGAATTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

522

Amino Acids

59.66

Weight (kDa)

5.21

Isoelectric Point (pI)

41.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 221, 497
AccII CGCG 3 cut(s) 93, 394, 684
AccIII TCCGGA 2 cut(s) 653, 1225
AciI CCGC 9 cut(s) 91, 186, 288, 394, 485, 684, 944, 1399, 1555
AclWI GGATC 2 cut(s) 878, 1310
AcoI YGGCCR 2 cut(s) 128, 739
AcsI RAATTY 2 cut(s) 443, 1346
AcuI CTGAAG 1 cut(s) 789
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 1 cut(s) 1247
AfiI CCNNNNNNNGG 2 cut(s) 241, 567
AflIII ACRYGT 1 cut(s) 329
AgsI TTSAA 7 cut(s) 506, 1132, 1145, 1181, 1187, 1289, 1385
AjiI CACGTC 1 cut(s) 330
AjnI CCWGG 2 cut(s) 947, 1550
AluBI AGCT 9 cut(s) 121, 434, 556, 731, 865, 1008, 1161, 1329, 1438
AluI AGCT 9 cut(s) 121, 434, 556, 731, 865, 1008, 1161, 1329, 1438
Alw21I GWGCWC 1 cut(s) 402
Alw26I GTCTC 2 cut(s) 698, 932
AlwI GGATC 2 cut(s) 878, 1310
Ama87I CYCGRG 1 cut(s) 407
Aor13HI TCCGGA 2 cut(s) 653, 1225
AoxI GGCC 4 cut(s) 128, 739, 945, 973
ApeKI GCWGC 3 cut(s) 32, 35, 1106
ApoI RAATTY 2 cut(s) 443, 1346
AspLEI GCGC 2 cut(s) 155, 224
AsuHPI GGTGA 2 cut(s) 34, 800
AvaI CYCGRG 1 cut(s) 407
BalI TGGCCA 2 cut(s) 130, 741
BanI GGYRCC 2 cut(s) 221, 497
BauI CACGAG 1 cut(s) 1267
Bbv12I GWGCWC 1 cut(s) 402
BbvI GCAGC 3 cut(s) 44, 47, 1093
BccI CCATC 6 cut(s) 47, 244, 540, 1247, 1415, 1537
BceAI ACGGC 1 cut(s) 258
BcgI CGANNNNNNTGC 2 cut(s) 1384, 1418
BciT130I CCWGG 2 cut(s) 949, 1552
BclI TGATCA 1 cut(s) 1207
BcoDI GTCTC 2 cut(s) 698, 932
BfmI CTRYAG 2 cut(s) 747, 1020
BfoI RGCGCY 1 cut(s) 225
BglII AGATCT 1 cut(s) 1170
BisI GCNGC 6 cut(s) 33, 36, 945, 1107, 1399, 1556
BlsI GCNGC 6 cut(s) 34, 37, 946, 1108, 1400, 1557
Bme1390I CCNGG 2 cut(s) 949, 1552
BmeT110I CYCGRG 1 cut(s) 407
BmgBI CACGTC 1 cut(s) 330
BmiI GGNNCC 4 cut(s) 184, 223, 499, 1500
BmrFI CCNGG 2 cut(s) 949, 1552
BmsI GCATC 4 cut(s) 919, 1039, 1351, 1514
Bpu10I CCTNAGC 1 cut(s) 1157
BpuEI CTTGAG 1 cut(s) 1358
BsaBI GATNNNNATC 3 cut(s) 381, 882, 1528
BsaHI GRCGYC 1 cut(s) 222
BsaJI CCNNGG 3 cut(s) 617, 839, 948
BsaWI WCCGGW 2 cut(s) 653, 1225
BsaXI ACNNNNNCTCC 4 cut(s) 90, 120, 679, 709
Bsc4I CCNNNNNNNGG 2 cut(s) 241, 567
Bse118I RCCGGY 1 cut(s) 269
Bse3DI GCAATG 3 cut(s) 891, 896, 1423
Bse8I GATNNNNATC 3 cut(s) 381, 882, 1528
BseAI TCCGGA 2 cut(s) 653, 1225
BseBI CCWGG 2 cut(s) 949, 1552
BseDI CCNNGG 3 cut(s) 617, 839, 948
BseGI GGATG 8 cut(s) 255, 495, 523, 551, 850, 880, 910, 1054
BseJI GATNNNNATC 3 cut(s) 381, 882, 1528
BseLI CCNNNNNNNGG 2 cut(s) 241, 567
BseMI GCAATG 3 cut(s) 891, 896, 1423
BseMII CTCAG 3 cut(s) 426, 1158, 1163
BseRI GAGGAG 2 cut(s) 17, 155
BseXI GCAGC 3 cut(s) 44, 47, 1093
Bsh1236I CGCG 3 cut(s) 93, 394, 684
BshFI GGCC 4 cut(s) 130, 741, 947, 975
BshNI GGYRCC 2 cut(s) 221, 497
BsiHKAI GWGCWC 1 cut(s) 402
BsiHKCI CYCGRG 1 cut(s) 407
BsiSI CCGG 3 cut(s) 270, 654, 1226
BslFI GGGAC 2 cut(s) 178, 1439
BslI CCNNNNNNNGG 2 cut(s) 241, 567
BsmAI GTCTC 2 cut(s) 698, 932
BsmFI GGGAC 2 cut(s) 178, 1439
BsnI GGCC 4 cut(s) 130, 741, 947, 975
BsoBI CYCGRG 1 cut(s) 407
Bsp1286I GDGCHC 1 cut(s) 402
Bsp13I TCCGGA 2 cut(s) 653, 1225
Bsp1407I TGTACA 1 cut(s) 1245
Bsp143I GATC 5 cut(s) 160, 883, 1170, 1207, 1315
Bsp19I CCATGG 1 cut(s) 839
BspACI CCGC 9 cut(s) 91, 186, 288, 394, 485, 684, 944, 1399, 1555
BspANI GGCC 4 cut(s) 130, 741, 947, 975
BspCNI CTCAG 3 cut(s) 427, 1159, 1162
BspEI TCCGGA 2 cut(s) 653, 1225
BspFNI CGCG 3 cut(s) 93, 394, 684
BspLI GGNNCC 4 cut(s) 184, 223, 499, 1500
BspMAI CTGCAG 1 cut(s) 751
BspPI GGATC 2 cut(s) 878, 1310
BspQI GCTCTTC 1 cut(s) 62
BspT107I GGYRCC 2 cut(s) 221, 497
BsrDI GCAATG 3 cut(s) 891, 896, 1423
BsrFI RCCGGY 1 cut(s) 269
BsrGI TGTACA 1 cut(s) 1245
BssAI RCCGGY 1 cut(s) 269
BssECI CCNNGG 3 cut(s) 617, 839, 948
BssMI GATC 5 cut(s) 160, 883, 1170, 1207, 1315
BssNI GRCGYC 1 cut(s) 222
BssSI CACGAG 1 cut(s) 1267
BssT1I CCWWGG 2 cut(s) 617, 839
Bst2BI CACGAG 1 cut(s) 1267
Bst2UI CCWGG 2 cut(s) 949, 1552
Bst4CI ACNGT 4 cut(s) 367, 1078, 1250, 1432
Bst6I CTCTTC 2 cut(s) 62, 300
BstACI GRCGYC 1 cut(s) 222
BstAUI TGTACA 1 cut(s) 1245
BstC8I GCNNGC 5 cut(s) 226, 483, 739, 1327, 1403
BstDEI CTNAG 5 cut(s) 435, 675, 1149, 1157, 1167
BstDSI CCRYGG 1 cut(s) 839
BstF5I GGATG 8 cut(s) 255, 495, 523, 551, 850, 880, 910, 1054
BstFNI CGCG 3 cut(s) 93, 394, 684
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 2 cut(s) 155, 224
BstKTI GATC 5 cut(s) 163, 886, 1173, 1210, 1318
BstMAI GTCTC 2 cut(s) 698, 932
BstMBI GATC 5 cut(s) 160, 883, 1170, 1207, 1315
BstMWI GCNNNNNNNGC 4 cut(s) 127, 219, 907, 972
BstNI CCWGG 2 cut(s) 949, 1552
BstNSI RCATGY 1 cut(s) 1372
BstSCI CCNGG 2 cut(s) 947, 1550
BstSFI CTRYAG 2 cut(s) 747, 1020
BstUI CGCG 3 cut(s) 93, 394, 684
BstV1I GCAGC 3 cut(s) 44, 47, 1093
BstX2I RGATCY 1 cut(s) 1170
BstXI CCANNNNNNTGG 1 cut(s) 983
BstYI RGATCY 1 cut(s) 1170
BsuRI GGCC 4 cut(s) 130, 741, 947, 975
BtgI CCRYGG 1 cut(s) 839
BtrI CACGTC 1 cut(s) 330
BtsCI GGATG 8 cut(s) 255, 495, 523, 551, 850, 880, 910, 1054
BtsI GCAGTG 1 cut(s) 1532
BtsIMutI CAGTG 4 cut(s) 636, 730, 1255, 1532
Cac8I GCNNGC 5 cut(s) 226, 483, 739, 1327, 1403
CfoI GCGC 2 cut(s) 155, 224
Cfr10I RCCGGY 1 cut(s) 269
Csp6I GTAC 1 cut(s) 1246
CspCI CAANNNNNGTGG 4 cut(s) 494, 529, 1026, 1061
CviAII CATG 9 cut(s) 126, 372, 478, 840, 967, 997, 1055, 1369, 1495
CviQI GTAC 1 cut(s) 1246
DdeI CTNAG 5 cut(s) 435, 675, 1149, 1157, 1167
DinI GGCGCC 1 cut(s) 223
DpnI GATC 5 cut(s) 162, 885, 1172, 1209, 1317
DpnII GATC 5 cut(s) 160, 883, 1170, 1207, 1315
EaeI YGGCCR 2 cut(s) 128, 739
Eam1104I CTCTTC 2 cut(s) 62, 300
EarI CTCTTC 2 cut(s) 62, 300
EciI GGCGGA 1 cut(s) 303
Eco130I CCWWGG 2 cut(s) 617, 839
Eco32I GATATC 1 cut(s) 919
Eco57I CTGAAG 1 cut(s) 789
Eco88I CYCGRG 1 cut(s) 407
EcoRII CCWGG 2 cut(s) 947, 1550
EcoRV GATATC 1 cut(s) 919
EcoT14I CCWWGG 2 cut(s) 617, 839
EgeI GGCGCC 1 cut(s) 223
EheI GGCGCC 1 cut(s) 223
ErhI CCWWGG 2 cut(s) 617, 839
FaeI CATG 9 cut(s) 129, 375, 481, 843, 970, 1000, 1058, 1372, 1498
FaqI GGGAC 2 cut(s) 178, 1439
FatI CATG 9 cut(s) 125, 371, 477, 839, 966, 996, 1054, 1368, 1494
FauI CCCGC 4 cut(s) 98, 387, 478, 677
FauNDI CATATG 4 cut(s) 585, 982, 1102, 1512
FbaI TGATCA 1 cut(s) 1207
Fnu4HI GCNGC 6 cut(s) 33, 36, 945, 1107, 1399, 1556
FokI GGATG 8 cut(s) 262, 502, 530, 558, 857, 887, 897, 1061
Fsp4HI GCNGC 6 cut(s) 33, 36, 945, 1107, 1399, 1556
GlaI GCGC 2 cut(s) 154, 223
GluI GCNGC 6 cut(s) 33, 36, 945, 1107, 1399, 1556
HaeII RGCGCY 1 cut(s) 225
HaeIII GGCC 4 cut(s) 130, 741, 947, 975
HapII CCGG 3 cut(s) 270, 654, 1226
HhaI GCGC 2 cut(s) 155, 224
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 9 cut(s) 129, 375, 481, 843, 970, 1000, 1058, 1372, 1498
Hin6I GCGC 2 cut(s) 153, 222
HinP1I GCGC 2 cut(s) 153, 222
HincII GTYRAC 1 cut(s) 1263
HindII GTYRAC 1 cut(s) 1263
HindIII AAGCTT 1 cut(s) 1436
HinfI GANTC 6 cut(s) 679, 764, 791, 878, 1084, 1391
HpaII CCGG 3 cut(s) 270, 654, 1226
HphI GGTGA 2 cut(s) 34, 800
Hpy166II GTNNAC 4 cut(s) 423, 534, 649, 1263
Hpy188I TCNGA 5 cut(s) 769, 883, 923, 1152, 1452
Hpy188III TCNNGA 6 cut(s) 654, 795, 914, 1166, 1226, 1286
Hpy8I GTNNAC 4 cut(s) 423, 534, 649, 1263
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 5 cut(s) 119, 512, 764, 965, 1288
HpyCH4III ACNGT 4 cut(s) 367, 1078, 1250, 1432
HpyCH4IV ACGT 2 cut(s) 329, 607
HpyF10VI GCNNNNNNNGC 4 cut(s) 127, 219, 907, 972
HpyF3I CTNAG 5 cut(s) 435, 675, 1149, 1157, 1167
HpySE526I ACGT 2 cut(s) 329, 607
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 9 cut(s) 129, 375, 481, 843, 970, 1000, 1058, 1372, 1498
HspAI GCGC 2 cut(s) 153, 222
KasI GGCGCC 1 cut(s) 221
Kpn2I TCCGGA 2 cut(s) 653, 1225
Ksp22I TGATCA 1 cut(s) 1207
Kzo9I GATC 5 cut(s) 160, 883, 1170, 1207, 1315
LguI GCTCTTC 1 cut(s) 62
LmnI GCTCC 4 cut(s) 188, 397, 728, 963
Lsp1109I GCAGC 3 cut(s) 44, 47, 1093
LweI GCATC 4 cut(s) 919, 1039, 1351, 1514
MaeII ACGT 2 cut(s) 329, 607
MaeIII GTNAC 3 cut(s) 367, 622, 1339
MalI GATC 5 cut(s) 162, 885, 1172, 1209, 1317
MboI GATC 5 cut(s) 160, 883, 1170, 1207, 1315
MboII GAAGA 4 cut(s) 49, 287, 377, 830
MfeI CAATTG 2 cut(s) 1211, 1320
MflI RGATCY 1 cut(s) 1170
MhlI GDGCHC 1 cut(s) 402
MlsI TGGCCA 2 cut(s) 130, 741
MluCI AATT 9 cut(s) 74, 278, 443, 1211, 1221, 1320, 1346, 1475, 1561
MluNI TGGCCA 2 cut(s) 130, 741
Mly113I GGCGCC 1 cut(s) 222
MmeI TCCRAC 1 cut(s) 305
Mox20I TGGCCA 2 cut(s) 130, 741
MroI TCCGGA 2 cut(s) 653, 1225
MscI TGGCCA 2 cut(s) 130, 741
MseI TTAA 5 cut(s) 414, 450, 758, 933, 1455
MslI CAYNNNNRTG 2 cut(s) 588, 1515
Msp20I TGGCCA 2 cut(s) 130, 741
MspA1I CMGCKG 1 cut(s) 1008
MspI CCGG 3 cut(s) 270, 654, 1226
MspR9I CCNGG 2 cut(s) 949, 1552
MunI CAATTG 2 cut(s) 1211, 1320
MvaI CCWGG 2 cut(s) 949, 1552
MvnI CGCG 3 cut(s) 93, 394, 684
MwoI GCNNNNNNNGC 4 cut(s) 127, 219, 907, 972
NarI GGCGCC 1 cut(s) 222
NcoI CCATGG 1 cut(s) 839
NdeI CATATG 4 cut(s) 585, 982, 1102, 1512
NdeII GATC 5 cut(s) 160, 883, 1170, 1207, 1315
NlaIII CATG 9 cut(s) 129, 375, 481, 843, 970, 1000, 1058, 1372, 1498
NlaIV GGNNCC 4 cut(s) 184, 223, 499, 1500
NmeAIII GCCGAG 1 cut(s) 189
NmuCI GTSAC 1 cut(s) 367
NspI RCATGY 1 cut(s) 1372
PaeR7I CTCGAG 1 cut(s) 407
PciSI GCTCTTC 1 cut(s) 62
PfeI GAWTC 6 cut(s) 679, 764, 791, 878, 1084, 1391
PkrI GCNGC 6 cut(s) 34, 37, 946, 1108, 1400, 1557
PluTI GGCGCC 1 cut(s) 225
Psp6I CCWGG 2 cut(s) 947, 1550
PspGI CCWGG 2 cut(s) 947, 1550
PspN4I GGNNCC 4 cut(s) 184, 223, 499, 1500
PspXI VCTCGAGB 1 cut(s) 407
PstI CTGCAG 1 cut(s) 751
PsuI RGATCY 1 cut(s) 1170
PvuII CAGCTG 1 cut(s) 1008
RsaI GTAC 1 cut(s) 1247
RsaNI GTAC 1 cut(s) 1246
RseI CAYNNNNRTG 2 cut(s) 588, 1515
SapI GCTCTTC 1 cut(s) 62
SaqAI TTAA 5 cut(s) 414, 450, 758, 933, 1455
SatI GCNGC 6 cut(s) 33, 36, 945, 1107, 1399, 1556
Sau3AI GATC 5 cut(s) 160, 883, 1170, 1207, 1315
ScrFI CCNGG 2 cut(s) 949, 1552
SduI GDGCHC 1 cut(s) 402
SfaNI GCATC 4 cut(s) 919, 1039, 1351, 1514
SfcI CTRYAG 2 cut(s) 747, 1020
SfoI GGCGCC 1 cut(s) 223
Sfr274I CTCGAG 1 cut(s) 407
SlaI CTCGAG 1 cut(s) 407
SmiMI CAYNNNNRTG 2 cut(s) 588, 1515
SmlI CTYRAG 2 cut(s) 407, 1373
SmoI CTYRAG 2 cut(s) 407, 1373
Sse9I AATT 9 cut(s) 74, 278, 443, 1211, 1221, 1320, 1346, 1475, 1561
SsiI CCGC 9 cut(s) 91, 186, 288, 394, 485, 684, 944, 1399, 1555
SspDI GGCGCC 1 cut(s) 221
SspI AATATT 2 cut(s) 565, 631
StyD4I CCNGG 2 cut(s) 947, 1550
StyI CCWWGG 2 cut(s) 617, 839
TaaI ACNGT 4 cut(s) 367, 1078, 1250, 1432
TaiI ACGT 2 cut(s) 332, 610
TaqI TCGA 4 cut(s) 159, 408, 1394, 1468
TaqII GACCGA 1 cut(s) 58
TasI AATT 9 cut(s) 74, 278, 443, 1211, 1221, 1320, 1346, 1475, 1561
TatI WGTACW 1 cut(s) 1245
TauI GCSGC 3 cut(s) 947, 1401, 1558
TfiI GAWTC 6 cut(s) 679, 764, 791, 878, 1084, 1391
Tru1I TTAA 5 cut(s) 414, 450, 758, 933, 1455
Tru9I TTAA 5 cut(s) 414, 450, 758, 933, 1455
TscAI CASTG 4 cut(s) 643, 730, 1255, 1539
TseFI GTSAC 1 cut(s) 367
TseI GCWGC 3 cut(s) 32, 35, 1106
Tsp45I GTSAC 1 cut(s) 367
TspDTI ATGAA 7 cut(s) 287, 456, 816, 1041, 1137, 1270, 1529
TspGWI ACGGA 1 cut(s) 1431
TspRI CASTG 4 cut(s) 643, 730, 1255, 1539
XapI RAATTY 2 cut(s) 443, 1346
XceI RCATGY 1 cut(s) 1372
XcmI CCANNNNNNNNNTGG 1 cut(s) 846
XhoI CTCGAG 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.