Rh5DG058300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0016784
Physical Location & Seq
Reverse (-)
4930878 .. 4931291
414 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG058300.1

Sequence Viewer

Length: 414 bp
ATGCCATCCACATTGGCGCCAGAGACCCTGGCGGGAAGCAACGGTCAAAAACAAAATATGAACCATGAAGAGGAGGATAAACACAGTACGGATACCGTGGATTCAGAACCAAGCACTAGCCTCGACACGAAACCAAGGTCACCAGACCCAAAGGATGACAGTGCAACCCCAATTTATGTCCCTGTACCAGTCCCAGACCCAAACAATGCTCCGTCATCACGGGGAAGACCTTGGCCACCAATAGATCCTCCGACACCACCAAGCAACACCCTGGGATCAAGCAATGCCCTGGCACCAGAACCAAGCGATGCCCTGGAACTGGAACCAAGCTATGATTCCGTGGTCCCAAAACCAAGTGAGGACCTGGAAGTAGAACTAAGCGTGGTCCCAAAACCGGCACCGGGATTAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

137

Amino Acids

14.48

Weight (kDa)

4.21

Isoelectric Point (pI)

71.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016784)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g09400 FvH4_3g09400
prunus_persica Prupe.4G045400_v2.0.a1
pyrus_communis pycom05g29190
rosa_laevigata RLG00000031547
rosa_multiflora Rmu_co8070730.1_g000001 Rmu_sc0001626.1_g000021 Rmu_sc0024443.1_g000001
rosa_roxburghii Rroxscaffold_1G00068280
rosa_rugosa Rorug04G0434300
rosa_samantha Rh5AG062200 Rh5BG058900 Rh5CG069400 Rh5DG058300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 16, 292, 397
AciI CCGC 1 cut(s) 32
AclWI GGATC 2 cut(s) 239, 283
AcoI YGGCCR 1 cut(s) 233
AcyI GRCGYC 1 cut(s) 17
AfaI GTAC 2 cut(s) 88, 186
AfiI CCNNNNNNNGG 4 cut(s) 70, 319, 394, 401
AjnI CCWGG 5 cut(s) 27, 270, 288, 312, 363
AjuI GAANNNNNNNTTGG 2 cut(s) 319, 351
AluBI AGCT 1 cut(s) 330
AluI AGCT 1 cut(s) 330
Alw26I GTCTC 1 cut(s) 17
AlwI GGATC 2 cut(s) 239, 283
AoxI GGCC 1 cut(s) 233
ArsI GACNNNNNNTTYG 2 cut(s) 122, 154
AspLEI GCGC 1 cut(s) 19
AspS9I GGNCC 3 cut(s) 343, 361, 385
AsuC2I CCSGG 1 cut(s) 402
AsuHPI GGTGA 1 cut(s) 132
AvaII GGWCC 3 cut(s) 343, 361, 385
BalI TGGCCA 1 cut(s) 235
BanI GGYRCC 3 cut(s) 16, 292, 397
BbsI GAAGAC 1 cut(s) 232
BccI CCATC 1 cut(s) 13
BcgI CGANNNNNNTGC 2 cut(s) 103, 137
BciT130I CCWGG 5 cut(s) 29, 272, 290, 314, 365
BciVI GTATCC 1 cut(s) 85
BcnI CCSGG 1 cut(s) 402
BcoDI GTCTC 1 cut(s) 17
BfaI CTAG 2 cut(s) 117, 412
BfoI RGCGCY 1 cut(s) 20
BfuI GTATCC 1 cut(s) 85
Bme1390I CCNGG 6 cut(s) 29, 272, 290, 314, 365, 402
Bme18I GGWCC 3 cut(s) 343, 361, 385
BmgT120I GGNCC 3 cut(s) 343, 361, 385
BmiI GGNNCC 6 cut(s) 18, 294, 324, 345, 387, 399
BmrFI CCNGG 6 cut(s) 29, 272, 290, 314, 365, 402
BmsI GCATC 1 cut(s) 298
BpiI GAAGAC 1 cut(s) 232
BpuMI CCSGG 1 cut(s) 402
BsaHI GRCGYC 1 cut(s) 17
BsaI GGTCTC 1 cut(s) 17
BsaJI CCNNGG 9 cut(s) 27, 96, 134, 230, 270, 271, 288, 312, 339
Bsc4I CCNNNNNNNGG 4 cut(s) 70, 319, 394, 401
Bse118I RCCGGY 1 cut(s) 394
Bse1I ACTGG 2 cut(s) 188, 324
Bse3DI GCAATG 1 cut(s) 289
BseBI CCWGG 5 cut(s) 29, 272, 290, 314, 365
BseDI CCNNGG 9 cut(s) 27, 96, 134, 230, 270, 271, 288, 312, 339
BseGI GGATG 2 cut(s) 5, 160
BseLI CCNNNNNNNGG 4 cut(s) 70, 319, 394, 401
BseMI GCAATG 1 cut(s) 289
BseNI ACTGG 2 cut(s) 188, 324
BseRI GAGGAG 1 cut(s) 86
BshFI GGCC 1 cut(s) 235
BshNI GGYRCC 3 cut(s) 16, 292, 397
BsiSI CCGG 2 cut(s) 395, 401
BslFI GGGAC 4 cut(s) 164, 176, 329, 371
BslI CCNNNNNNNGG 4 cut(s) 70, 319, 394, 401
BsmAI GTCTC 1 cut(s) 17
BsmFI GGGAC 4 cut(s) 164, 176, 329, 371
BsnI GGCC 1 cut(s) 235
Bso31I GGTCTC 1 cut(s) 17
Bsp143I GATC 2 cut(s) 244, 275
BspACI CCGC 1 cut(s) 32
BspANI GGCC 1 cut(s) 235
BspLI GGNNCC 6 cut(s) 18, 294, 324, 345, 387, 399
BspPI GGATC 2 cut(s) 239, 283
BspT107I GGYRCC 3 cut(s) 16, 292, 397
BspTNI GGTCTC 1 cut(s) 17
BsrDI GCAATG 1 cut(s) 289
BsrFI RCCGGY 1 cut(s) 394
BsrI ACTGG 2 cut(s) 188, 324
BssAI RCCGGY 1 cut(s) 394
BssECI CCNNGG 9 cut(s) 27, 96, 134, 230, 270, 271, 288, 312, 339
BssMI GATC 2 cut(s) 244, 275
BssNI GRCGYC 1 cut(s) 17
BssT1I CCWWGG 2 cut(s) 134, 230
Bst2UI CCWGG 5 cut(s) 29, 272, 290, 314, 365
Bst4CI ACNGT 4 cut(s) 44, 86, 97, 161
Bst6I CTCTTC 1 cut(s) 63
BstACI GRCGYC 1 cut(s) 17
BstDEI CTNAG 1 cut(s) 377
BstDSI CCRYGG 2 cut(s) 96, 339
BstEII GGTNACC 1 cut(s) 138
BstF5I GGATG 2 cut(s) 5, 160
BstH2I RGCGCY 1 cut(s) 20
BstHHI GCGC 1 cut(s) 19
BstKTI GATC 2 cut(s) 247, 278
BstMAI GTCTC 1 cut(s) 17
BstMBI GATC 2 cut(s) 244, 275
BstNI CCWGG 5 cut(s) 29, 272, 290, 314, 365
BstPI GGTNACC 1 cut(s) 138
BstSCI CCNGG 6 cut(s) 27, 270, 288, 312, 363, 400
BstV2I GAAGAC 1 cut(s) 232
BstX2I RGATCY 1 cut(s) 244
BstYI RGATCY 1 cut(s) 244
BsuI GTATCC 1 cut(s) 85
BsuRI GGCC 1 cut(s) 235
BtgI CCRYGG 2 cut(s) 96, 339
BtgZI GCGATG 1 cut(s) 321
BtsCI GGATG 2 cut(s) 5, 160
BtsIMutI CAGTG 1 cut(s) 166
CfoI GCGC 1 cut(s) 19
Cfr10I RCCGGY 1 cut(s) 394
Cfr13I GGNCC 3 cut(s) 343, 361, 385
Csp6I GTAC 2 cut(s) 87, 185
CviAII CATG 1 cut(s) 65
CviJI RGCY 3 cut(s) 120, 235, 330
CviKI_1 RGCY 3 cut(s) 120, 235, 330
CviQI GTAC 2 cut(s) 87, 185
DdeI CTNAG 1 cut(s) 377
DinI GGCGCC 1 cut(s) 18
DpnI GATC 2 cut(s) 246, 277
DpnII GATC 2 cut(s) 244, 275
EaeI YGGCCR 1 cut(s) 233
Eam1104I CTCTTC 1 cut(s) 63
EarI CTCTTC 1 cut(s) 63
Eco130I CCWWGG 2 cut(s) 134, 230
Eco31I GGTCTC 1 cut(s) 17
Eco47I GGWCC 3 cut(s) 343, 361, 385
Eco91I GGTNACC 1 cut(s) 138
EcoO109I RGGNCCY 1 cut(s) 361
EcoO65I GGTNACC 1 cut(s) 138
EcoRII CCWGG 5 cut(s) 27, 270, 288, 312, 363
EcoT14I CCWWGG 2 cut(s) 134, 230
EgeI GGCGCC 1 cut(s) 18
EheI GGCGCC 1 cut(s) 18
ErhI CCWWGG 2 cut(s) 134, 230
FaeI CATG 1 cut(s) 68
FaiI YATR 4 cut(s) 59, 66, 177, 333
FaqI GGGAC 4 cut(s) 164, 176, 329, 371
FatI CATG 1 cut(s) 64
FauI CCCGC 1 cut(s) 25
FokI GGATG 1 cut(s) 167
FspBI CTAG 2 cut(s) 117, 412
GlaI GCGC 1 cut(s) 18
HaeII RGCGCY 1 cut(s) 20
HaeIII GGCC 1 cut(s) 235
HapII CCGG 2 cut(s) 395, 401
HhaI GCGC 1 cut(s) 19
Hin1I GRCGYC 1 cut(s) 17
Hin1II CATG 1 cut(s) 68
Hin6I GCGC 1 cut(s) 17
HinP1I GCGC 1 cut(s) 17
HinfI GANTC 2 cut(s) 101, 335
HpaII CCGG 2 cut(s) 395, 401
HphI GGTGA 1 cut(s) 132
Hpy188I TCNGA 2 cut(s) 106, 252
HpyCH4III ACNGT 4 cut(s) 44, 86, 97, 161
HpyCH4V TGCA 1 cut(s) 164
HpyF3I CTNAG 1 cut(s) 377
Hsp92I GRCGYC 1 cut(s) 17
Hsp92II CATG 1 cut(s) 68
HspAI GCGC 1 cut(s) 17
KasI GGCGCC 1 cut(s) 16
Kzo9I GATC 2 cut(s) 244, 275
LmnI GCTCC 1 cut(s) 214
LweI GCATC 1 cut(s) 298
MaeI CTAG 2 cut(s) 117, 412
MaeIII GTNAC 1 cut(s) 138
MalI GATC 2 cut(s) 246, 277
MboI GATC 2 cut(s) 244, 275
MboII GAAGA 2 cut(s) 80, 237
MflI RGATCY 1 cut(s) 244
MlsI TGGCCA 1 cut(s) 235
MluCI AATT 1 cut(s) 171
MluNI TGGCCA 1 cut(s) 235
Mly113I GGCGCC 1 cut(s) 17
MmeI TCCRAC 1 cut(s) 275
MnlI CCTC 5 cut(s) 64, 67, 131, 258, 352
Mox20I TGGCCA 1 cut(s) 235
MscI TGGCCA 1 cut(s) 235
Msp20I TGGCCA 1 cut(s) 235
MspI CCGG 2 cut(s) 395, 401
MspR9I CCNGG 6 cut(s) 29, 272, 290, 314, 365, 402
MvaI CCWGG 5 cut(s) 29, 272, 290, 314, 365
NarI GGCGCC 1 cut(s) 17
NciI CCSGG 1 cut(s) 402
NdeII GATC 2 cut(s) 244, 275
NlaIII CATG 1 cut(s) 68
NlaIV GGNNCC 6 cut(s) 18, 294, 324, 345, 387, 399
NmuCI GTSAC 1 cut(s) 138
PasI CCCWGGG 1 cut(s) 271
PfeI GAWTC 2 cut(s) 101, 335
PluTI GGCGCC 1 cut(s) 20
PpuMI RGGWCCY 1 cut(s) 361
Psp5II RGGWCCY 1 cut(s) 361
Psp6I CCWGG 5 cut(s) 27, 270, 288, 312, 363
PspEI GGTNACC 1 cut(s) 138
PspGI CCWGG 5 cut(s) 27, 270, 288, 312, 363
PspN4I GGNNCC 6 cut(s) 18, 294, 324, 345, 387, 399
PspPI GGNCC 3 cut(s) 343, 361, 385
PspPPI RGGWCCY 1 cut(s) 361
PsuI RGATCY 1 cut(s) 244
RsaI GTAC 2 cut(s) 88, 186
RsaNI GTAC 2 cut(s) 87, 185
Sau3AI GATC 2 cut(s) 244, 275
Sau96I GGNCC 3 cut(s) 343, 361, 385
ScrFI CCNGG 6 cut(s) 29, 272, 290, 314, 365, 402
SetI ASST 4 cut(s) 140, 232, 332, 366
SfaNI GCATC 1 cut(s) 298
SfoI GGCGCC 1 cut(s) 18
SinI GGWCC 3 cut(s) 343, 361, 385
Sse9I AATT 1 cut(s) 171
SsiI CCGC 1 cut(s) 32
SspDI GGCGCC 1 cut(s) 16
SspMI CTAG 2 cut(s) 117, 412
StyD4I CCNGG 6 cut(s) 27, 270, 288, 312, 363, 400
StyI CCWWGG 2 cut(s) 134, 230
TaaI ACNGT 4 cut(s) 44, 86, 97, 161
TaqI TCGA 1 cut(s) 123
TasI AATT 1 cut(s) 171
TfiI GAWTC 2 cut(s) 101, 335
TscAI CASTG 1 cut(s) 166
TseFI GTSAC 1 cut(s) 138
Tsp45I GTSAC 1 cut(s) 138
TspDTI ATGAA 2 cut(s) 74, 81
TspGWI ACGGA 3 cut(s) 104, 201, 328
TspRI CASTG 1 cut(s) 166
VpaK11BI GGWCC 3 cut(s) 343, 361, 385
XspI CTAG 2 cut(s) 117, 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.