Rh5DG468000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0015742
Physical Location & Seq
Reverse (-)
74219961 .. 74228545
8585 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG468000.1

Sequence Viewer

Length: 675 bp
ATGAAGGTCCCCAAGTCGCCGTATAAGGTTTTGAATTTGCAAGATGGTTTCAGGTTGGTTATATTAGCTTTATTGATTAGCTTCTGTGCTGGTTTCGACATTGTTGAGATCATTGTTGCTGTTATTGGTGATTTAGCTTATGAATTTTGTGAAGCCCAAGAAGAAGGTAGAGAGGAACTGGAATTAAATATCAAGGATGAATCTGATGAGTTCAGATTACCAACTGACCAGGAACTTGAACAAGAGGCGCATCAACCTCCTGATCTTTCACACCTTCAGGGGAGGTTAAAAGAGATCTGCTATTTTACCATGGTGAAGATTATCAAGGCTGGTCTTATAAGCGCTTATATCATAAAAACACTCTACTTTGGAAGGCATTCTACAGATCGTGAAGTTGCTCTCATGATGCTTCTGGCTTATTTGTCATACATGTTGGCTGAGGAAGACAAGGAGATACTCGAGAAACAGATGCATGGCATGGCTGTAGTGATTGAGAAATTGGAAAGCAGTAGACAAAAGCTTTTAATGGAGATTGATTCCCAATCTTCGGAGATAGAAAGGCTATTTGAGGAAAACTCTCATTTATCTAGTTCTCTTCGGGAGGCTATGAGTATAGCTCTGCACTGGGAGAATCAGACTATCTTAAGCAAAATGAAGCGCTCCGAGGAATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

224

Amino Acids

25.81

Weight (kDa)

4.82

Isoelectric Point (pI)

64.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 338
AccI GTMKAC 1 cut(s) 511
AcsI RAATTY 2 cut(s) 34, 143
AcuI CTGAAG 1 cut(s) 260
AfeI AGCGCT 2 cut(s) 343, 659
AfiI CCNNNNNNNGG 1 cut(s) 547
AflII CTTAAG 1 cut(s) 643
AflIII ACRYGT 1 cut(s) 429
AgsI TTSAA 2 cut(s) 34, 239
AjnI CCWGG 1 cut(s) 228
AluBI AGCT 5 cut(s) 68, 81, 137, 520, 617
AluI AGCT 5 cut(s) 68, 81, 137, 520, 617
Ama87I CYCGRG 1 cut(s) 458
Aor51HI AGCGCT 2 cut(s) 343, 659
ApoI RAATTY 2 cut(s) 34, 143
Asp700I GAANNNNTTC 1 cut(s) 376
AspLEI GCGC 3 cut(s) 250, 344, 660
AspS9I GGNCC 1 cut(s) 7
AsuHPI GGTGA 2 cut(s) 140, 325
AvaI CYCGRG 1 cut(s) 458
AvaII GGWCC 1 cut(s) 7
BarI GAAGNNNNNNTAC 2 cut(s) 364, 396
BbsI GAAGAC 1 cut(s) 450
BbvCI CCTCAGC 1 cut(s) 438
BccI CCATC 1 cut(s) 38
BceAI ACGGC 1 cut(s) 4
BciT130I CCWGG 1 cut(s) 230
BfaI CTAG 1 cut(s) 588
BfmI CTRYAG 2 cut(s) 381, 483
BfoI RGCGCY 2 cut(s) 345, 661
BfrI CTTAAG 1 cut(s) 643
BglII AGATCT 1 cut(s) 294
Bme1390I CCNGG 1 cut(s) 230
Bme18I GGWCC 1 cut(s) 7
BmeT110I CYCGRG 1 cut(s) 458
BmgT120I GGNCC 1 cut(s) 7
BmiI GGNNCC 1 cut(s) 9
BmrFI CCNGG 1 cut(s) 230
BmrI ACTGGG 1 cut(s) 634
BmsI GCATC 3 cut(s) 259, 396, 459
BmuI ACTGGG 1 cut(s) 634
BpiI GAAGAC 1 cut(s) 450
BplI GAGNNNNNCTC 4 cut(s) 560, 592, 601, 633
Bpu10I CCTNAGC 1 cut(s) 438
BsaJI CCNNGG 2 cut(s) 309, 663
Bsc4I CCNNNNNNNGG 1 cut(s) 547
Bse1I ACTGG 2 cut(s) 183, 629
BseBI CCWGG 1 cut(s) 230
BseDI CCNNGG 2 cut(s) 309, 663
BseGI GGATG 1 cut(s) 202
BseLI CCNNNNNNNGG 1 cut(s) 547
BseMII CTCAG 1 cut(s) 429
BseNI ACTGG 2 cut(s) 183, 629
BsgI GTGCAG 1 cut(s) 605
BsiHKCI CYCGRG 1 cut(s) 458
BslI CCNNNNNNNGG 1 cut(s) 547
BsmI GAATGC 1 cut(s) 376
BsoBI CYCGRG 1 cut(s) 458
Bsp143I GATC 4 cut(s) 108, 262, 294, 385
Bsp19I CCATGG 1 cut(s) 309
BspCNI CTCAG 1 cut(s) 430
BspHI TCATGA 1 cut(s) 402
BspLI GGNNCC 1 cut(s) 9
BspTI CTTAAG 1 cut(s) 643
BsrI ACTGG 2 cut(s) 183, 629
BssECI CCNNGG 2 cut(s) 309, 663
BssMI GATC 4 cut(s) 108, 262, 294, 385
BssT1I CCWWGG 1 cut(s) 309
Bst2UI CCWGG 1 cut(s) 230
Bst6I CTCTTC 1 cut(s) 600
BstAFI CTTAAG 1 cut(s) 643
BstDEI CTNAG 2 cut(s) 438, 672
BstDSI CCRYGG 1 cut(s) 309
BstF5I GGATG 1 cut(s) 202
BstH2I RGCGCY 2 cut(s) 345, 661
BstHHI GCGC 3 cut(s) 250, 344, 660
BstKTI GATC 4 cut(s) 111, 265, 297, 388
BstMBI GATC 4 cut(s) 108, 262, 294, 385
BstNI CCWGG 1 cut(s) 230
BstNSI RCATGY 1 cut(s) 433
BstSCI CCNGG 1 cut(s) 228
BstSFI CTRYAG 2 cut(s) 381, 483
BstV2I GAAGAC 1 cut(s) 450
BstX2I RGATCY 1 cut(s) 294
BstYI RGATCY 1 cut(s) 294
BtgI CCRYGG 1 cut(s) 309
BtsCI GGATG 1 cut(s) 202
BtsIMutI CAGTG 1 cut(s) 622
CciI TCATGA 1 cut(s) 402
CfoI GCGC 3 cut(s) 250, 344, 660
Cfr13I GGNCC 1 cut(s) 7
CviAII CATG 5 cut(s) 310, 403, 430, 473, 478
DdeI CTNAG 2 cut(s) 438, 672
DpnI GATC 4 cut(s) 110, 264, 296, 387
DpnII GATC 4 cut(s) 108, 262, 294, 385
Eam1104I CTCTTC 1 cut(s) 600
EarI CTCTTC 1 cut(s) 600
Eco130I CCWWGG 1 cut(s) 309
Eco47I GGWCC 1 cut(s) 7
Eco47III AGCGCT 2 cut(s) 343, 659
Eco57I CTGAAG 1 cut(s) 260
Eco88I CYCGRG 1 cut(s) 458
EcoO109I RGGNCCY 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 228
EcoT14I CCWWGG 1 cut(s) 309
EcoT22I ATGCAT 1 cut(s) 474
ErhI CCWWGG 1 cut(s) 309
FaeI CATG 5 cut(s) 313, 406, 433, 476, 481
FatI CATG 5 cut(s) 309, 402, 429, 472, 477
FblI GTMKAC 1 cut(s) 511
FokI GGATG 1 cut(s) 209
FspBI CTAG 1 cut(s) 588
GlaI GCGC 3 cut(s) 249, 343, 659
HaeII RGCGCY 2 cut(s) 345, 661
HhaI GCGC 3 cut(s) 250, 344, 660
Hin1II CATG 5 cut(s) 313, 406, 433, 476, 481
Hin6I GCGC 3 cut(s) 248, 342, 658
HinP1I GCGC 3 cut(s) 248, 342, 658
HindIII AAGCTT 1 cut(s) 518
HinfI GANTC 4 cut(s) 200, 536, 631, 668
HphI GGTGA 2 cut(s) 140, 325
Hpy166II GTNNAC 1 cut(s) 512
Hpy188I TCNGA 5 cut(s) 205, 215, 550, 636, 664
Hpy188III TCNNGA 5 cut(s) 260, 389, 403, 460, 599
Hpy8I GTNNAC 1 cut(s) 512
HpyAV CCTTC 3 cut(s) 158, 284, 366
HpyCH4V TGCA 3 cut(s) 40, 472, 622
HpyF3I CTNAG 2 cut(s) 438, 672
Hsp92II CATG 5 cut(s) 313, 406, 433, 476, 481
HspAI GCGC 3 cut(s) 248, 342, 658
Kzo9I GATC 4 cut(s) 108, 262, 294, 385
LmnI GCTCC 1 cut(s) 665
LweI GCATC 3 cut(s) 259, 396, 459
MaeI CTAG 1 cut(s) 588
MalI GATC 4 cut(s) 110, 264, 296, 387
MboI GATC 4 cut(s) 108, 262, 294, 385
MboII GAAGA 5 cut(s) 173, 328, 455, 537, 587
MflI RGATCY 1 cut(s) 294
MluCI AATT 4 cut(s) 34, 143, 182, 497
MnlI CCTC 8 cut(s) 166, 238, 267, 276, 433, 562, 595, 658
Mph1103I ATGCAT 1 cut(s) 474
MroXI GAANNNNTTC 1 cut(s) 376
MseI TTAA 4 cut(s) 185, 287, 524, 644
MspCI CTTAAG 1 cut(s) 643
MspR9I CCNGG 1 cut(s) 230
Mva1269I GAATGC 1 cut(s) 376
MvaI CCWGG 1 cut(s) 230
NcoI CCATGG 1 cut(s) 309
NdeII GATC 4 cut(s) 108, 262, 294, 385
NlaIII CATG 5 cut(s) 313, 406, 433, 476, 481
NlaIV GGNNCC 1 cut(s) 9
NsiI ATGCAT 1 cut(s) 474
NspI RCATGY 1 cut(s) 433
PaeR7I CTCGAG 1 cut(s) 458
PagI TCATGA 1 cut(s) 402
PciI ACATGT 1 cut(s) 429
PctI GAATGC 1 cut(s) 376
PdmI GAANNNNTTC 1 cut(s) 376
PfeI GAWTC 4 cut(s) 200, 536, 631, 668
PpuMI RGGWCCY 1 cut(s) 7
PscI ACATGT 1 cut(s) 429
PsiI TTATAA 1 cut(s) 338
Psp5II RGGWCCY 1 cut(s) 7
Psp6I CCWGG 1 cut(s) 228
PspGI CCWGG 1 cut(s) 228
PspN4I GGNNCC 1 cut(s) 9
PspPI GGNCC 1 cut(s) 7
PspPPI RGGWCCY 1 cut(s) 7
PsuI RGATCY 1 cut(s) 294
SaqAI TTAA 4 cut(s) 185, 287, 524, 644
Sau3AI GATC 4 cut(s) 108, 262, 294, 385
Sau96I GGNCC 1 cut(s) 7
ScrFI CCNGG 1 cut(s) 230
SfaNI GCATC 3 cut(s) 259, 396, 459
SfcI CTRYAG 2 cut(s) 381, 483
Sfr274I CTCGAG 1 cut(s) 458
SinI GGWCC 1 cut(s) 7
SlaI CTCGAG 1 cut(s) 458
SmlI CTYRAG 2 cut(s) 458, 643
SmoI CTYRAG 2 cut(s) 458, 643
Sse9I AATT 4 cut(s) 34, 143, 182, 497
SspMI CTAG 1 cut(s) 588
StyD4I CCNGG 1 cut(s) 228
StyI CCWWGG 1 cut(s) 309
TaqI TCGA 2 cut(s) 96, 459
TasI AATT 4 cut(s) 34, 143, 182, 497
TfiI GAWTC 4 cut(s) 200, 536, 631, 668
Tru1I TTAA 4 cut(s) 185, 287, 524, 644
Tru9I TTAA 4 cut(s) 185, 287, 524, 644
TscAI CASTG 1 cut(s) 629
TspDTI ATGAA 4 cut(s) 17, 156, 213, 668
TspRI CASTG 1 cut(s) 629
Vha464I CTTAAG 1 cut(s) 643
VpaK11BI GGWCC 1 cut(s) 7
XapI RAATTY 2 cut(s) 34, 143
XceI RCATGY 1 cut(s) 433
XhoI CTCGAG 1 cut(s) 458
XmiI GTMKAC 1 cut(s) 511
XmnI GAANNNNTTC 1 cut(s) 376
XspI CTAG 1 cut(s) 588
Zsp2I ATGCAT 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.