Rh6BG059900

3-oxo-Delta(4,5)-steroid 5-beta-reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0018197
Physical Location & Seq
Reverse (-)
9517050 .. 9517432
383 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG059900.1

Sequence Viewer

Length: 303 bp
ATGAGTTGGTGGTGGGCAGGAGCCATAGGAGCTGCAAAGAAAAAGTTTGATCCAGACGATGCACCAACCGAGTACCGAGCGTGGCCCTGGTACTTGGGGTCACCGGAATCGTCGGAAACAGCCTCGCCGAGAATCTCCCACTTGCCAATCGCGACACTCCCAGCGTGCCGTGGAAGGTCTACGGCGTGGCCCGGAGACCCCGCCCGCAGTGGAACGCTGACCGCCCGATTCAGTACATCCAGTGTGACCTCTCTGACTCTAAACAGACAGAGGCAGAGCTGTCCCAGCTGGCCAGCTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

100

Amino Acids

10.97

Weight (kDa)

9.23

Isoelectric Point (pI)

65.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 179
AccII CGCG 1 cut(s) 152
AciI CCGC 3 cut(s) 201, 205, 222
AclWI GGATC 1 cut(s) 44
AcoI YGGCCR 1 cut(s) 290
AfaI GTAC 3 cut(s) 74, 92, 235
AjnI CCWGG 1 cut(s) 86
AluBI AGCT 4 cut(s) 32, 279, 288, 296
AluI AGCT 4 cut(s) 32, 279, 288, 296
Alw26I GTCTC 1 cut(s) 189
AlwI GGATC 1 cut(s) 44
AoxI GGCC 3 cut(s) 83, 188, 290
ApeKI GCWGC 1 cut(s) 32
AspS9I GGNCC 2 cut(s) 84, 189
AsuC2I CCSGG 1 cut(s) 192
AsuHPI GGTGA 1 cut(s) 93
BalI TGGCCA 1 cut(s) 292
BbvI GCAGC 1 cut(s) 19
BceAI ACGGC 2 cut(s) 153, 198
BciT130I CCWGG 1 cut(s) 88
BcnI CCSGG 1 cut(s) 192
BcoDI GTCTC 1 cut(s) 189
BisI GCNGC 1 cut(s) 33
BlsI GCNGC 1 cut(s) 34
Bme1390I CCNGG 2 cut(s) 88, 192
BmgT120I GGNCC 2 cut(s) 84, 189
BmiI GGNNCC 1 cut(s) 22
BmrFI CCNGG 2 cut(s) 88, 192
BmsI GCATC 1 cut(s) 49
BpuMI CCSGG 1 cut(s) 192
BsaI GGTCTC 1 cut(s) 189
BsaJI CCNNGG 2 cut(s) 86, 169
BsaWI WCCGGW 1 cut(s) 103
Bse1I ACTGG 1 cut(s) 240
BseBI CCWGG 1 cut(s) 88
BseDI CCNNGG 2 cut(s) 86, 169
BseGI GGATG 1 cut(s) 236
BseNI ACTGG 1 cut(s) 240
BseXI GCAGC 1 cut(s) 19
BseYI CCCAGC 2 cut(s) 160, 284
Bsh1236I CGCG 1 cut(s) 152
BshFI GGCC 3 cut(s) 85, 190, 292
BsiSI CCGG 2 cut(s) 104, 192
BslFI GGGAC 1 cut(s) 267
BsmAI GTCTC 1 cut(s) 189
BsmFI GGGAC 1 cut(s) 267
BsnI GGCC 3 cut(s) 85, 190, 292
Bso31I GGTCTC 1 cut(s) 189
Bsp143I GATC 1 cut(s) 49
Bsp68I TCGCGA 1 cut(s) 152
BspACI CCGC 3 cut(s) 201, 205, 222
BspANI GGCC 3 cut(s) 85, 190, 292
BspFNI CGCG 1 cut(s) 152
BspLI GGNNCC 1 cut(s) 22
BspPI GGATC 1 cut(s) 44
BspTNI GGTCTC 1 cut(s) 189
BsrI ACTGG 1 cut(s) 240
BssECI CCNNGG 2 cut(s) 86, 169
BssMI GATC 1 cut(s) 49
Bst2UI CCWGG 1 cut(s) 88
BstC8I GCNNGC 4 cut(s) 166, 205, 290, 294
BstDSI CCRYGG 1 cut(s) 169
BstEII GGTNACC 1 cut(s) 99
BstF5I GGATG 1 cut(s) 236
BstFNI CGCG 1 cut(s) 152
BstKTI GATC 1 cut(s) 52
BstMAI GTCTC 1 cut(s) 189
BstMBI GATC 1 cut(s) 49
BstMWI GCNNNNNNNGC 2 cut(s) 29, 285
BstNI CCWGG 1 cut(s) 88
BstPI GGTNACC 1 cut(s) 99
BstSCI CCNGG 2 cut(s) 86, 190
BstUI CGCG 1 cut(s) 152
BstV1I GCAGC 1 cut(s) 19
BsuRI GGCC 3 cut(s) 85, 190, 292
BtgI CCRYGG 1 cut(s) 169
BtsCI GGATG 1 cut(s) 236
BtsI GCAGTG 1 cut(s) 214
BtsIMutI CAGTG 2 cut(s) 214, 247
BtuMI TCGCGA 1 cut(s) 152
Cac8I GCNNGC 4 cut(s) 166, 205, 290, 294
Cfr13I GGNCC 2 cut(s) 84, 189
Csp6I GTAC 3 cut(s) 73, 91, 234
CviJI RGCY 9 cut(s) 23, 32, 85, 122, 190, 279, 288, 292, 296
CviKI_1 RGCY 9 cut(s) 23, 32, 85, 122, 190, 279, 288, 292, 296
CviQI GTAC 3 cut(s) 73, 91, 234
DpnI GATC 1 cut(s) 51
DpnII GATC 1 cut(s) 49
EaeI YGGCCR 1 cut(s) 290
Eco31I GGTCTC 1 cut(s) 189
Eco91I GGTNACC 1 cut(s) 99
EcoO65I GGTNACC 1 cut(s) 99
EcoRII CCWGG 1 cut(s) 86
FaiI YATR 1 cut(s) 26
FaqI GGGAC 1 cut(s) 267
FauI CCCGC 2 cut(s) 208, 212
FblI GTMKAC 1 cut(s) 179
Fnu4HI GCNGC 1 cut(s) 33
FokI GGATG 1 cut(s) 223
Fsp4HI GCNGC 1 cut(s) 33
GluI GCNGC 1 cut(s) 33
GsaI CCCAGC 2 cut(s) 164, 288
HaeIII GGCC 3 cut(s) 85, 190, 292
HapII CCGG 2 cut(s) 104, 192
HinfI GANTC 4 cut(s) 107, 132, 228, 256
HpaII CCGG 2 cut(s) 104, 192
HphI GGTGA 1 cut(s) 93
Hpy166II GTNNAC 1 cut(s) 180
Hpy188I TCNGA 2 cut(s) 115, 255
Hpy188III TCNNGA 2 cut(s) 53, 151
Hpy8I GTNNAC 1 cut(s) 180
Hpy99I CGWCG 1 cut(s) 115
HpyAV CCTTC 1 cut(s) 168
HpyCH4V TGCA 2 cut(s) 35, 62
HpyF10VI GCNNNNNNNGC 2 cut(s) 29, 285
Kzo9I GATC 1 cut(s) 49
LmnI GCTCC 2 cut(s) 20, 29
Lsp1109I GCAGC 1 cut(s) 19
LweI GCATC 1 cut(s) 49
MaeIII GTNAC 2 cut(s) 99, 244
MalI GATC 1 cut(s) 51
MboI GATC 1 cut(s) 49
MlsI TGGCCA 1 cut(s) 292
MluNI TGGCCA 1 cut(s) 292
MlyI GAGTC 1 cut(s) 250
MmeI TCCRAC 1 cut(s) 93
MnlI CCTC 3 cut(s) 133, 259, 264
Mox20I TGGCCA 1 cut(s) 292
MscI TGGCCA 1 cut(s) 292
Msp20I TGGCCA 1 cut(s) 292
MspA1I CMGCKG 2 cut(s) 288, 296
MspI CCGG 2 cut(s) 104, 192
MspR9I CCNGG 2 cut(s) 88, 192
MvaI CCWGG 1 cut(s) 88
MvnI CGCG 1 cut(s) 152
MwoI GCNNNNNNNGC 2 cut(s) 29, 285
NciI CCSGG 1 cut(s) 192
NdeII GATC 1 cut(s) 49
NlaIV GGNNCC 1 cut(s) 22
NmeAIII GCCGAG 1 cut(s) 153
NmuCI GTSAC 2 cut(s) 99, 244
NruI TCGCGA 1 cut(s) 152
PfeI GAWTC 3 cut(s) 107, 132, 228
PkrI GCNGC 1 cut(s) 34
PleI GAGTC 1 cut(s) 250
PpsI GAGTC 1 cut(s) 250
Psp6I CCWGG 1 cut(s) 86
PspEI GGTNACC 1 cut(s) 99
PspFI CCCAGC 2 cut(s) 160, 284
PspGI CCWGG 1 cut(s) 86
PspN4I GGNNCC 1 cut(s) 22
PspPI GGNCC 2 cut(s) 84, 189
PvuII CAGCTG 2 cut(s) 288, 296
RruI TCGCGA 1 cut(s) 152
RsaI GTAC 3 cut(s) 74, 92, 235
RsaNI GTAC 3 cut(s) 73, 91, 234
SatI GCNGC 1 cut(s) 33
Sau3AI GATC 1 cut(s) 49
Sau96I GGNCC 2 cut(s) 84, 189
SchI GAGTC 1 cut(s) 250
ScrFI CCNGG 2 cut(s) 88, 192
SetI ASST 6 cut(s) 34, 179, 251, 281, 290, 298
SfaNI GCATC 1 cut(s) 49
SsiI CCGC 3 cut(s) 201, 205, 222
StyD4I CCNGG 2 cut(s) 86, 190
TatI WGTACW 1 cut(s) 233
TfiI GAWTC 3 cut(s) 107, 132, 228
TscAI CASTG 2 cut(s) 214, 247
TseFI GTSAC 2 cut(s) 99, 244
TseI GCWGC 1 cut(s) 32
Tsp45I GTSAC 2 cut(s) 99, 244
TspRI CASTG 2 cut(s) 214, 247
XmiI GTMKAC 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.