Rh6BG360400

DYW family of nucleic acid deaminases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0016646
Physical Location & Seq
Forward (+)
59098881 .. 59099420
540 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG360400.1

Sequence Viewer

Length: 408 bp
ATGTCCAATCCAAAGTTCCAATCCACTCCCCGGTCTCCATCTTCAATCGAATGCGCTTACATGGAGTTGAACCCGATAACCGCACCTTCCCTTTCCTCCTTCAATTCTTCAAATCCATTCCTTACTTCCAATCAGGAAAACGAATCCATGCCCAAATTCTTCTCTTCGGATGCGTATGCGAAAGTGGGTTTGATTGATGCTGCACGAGATGTGTTCAATGAAATGCCCAAGAGAAATGTGATTTCATGGGGTTGTATGATCAAGGCGTATGTAATGTGTGGTGGTTATAATTATAAGGAAGCACTTGCCTTGTTTCGTGAGATGCAGATGTTGGATAGTGTGAGGCCTAATGAGTTCACCATGTCGAGCATACTTTTGGCTTGTGGAAGATTGGGTGCGCGCACTTGA

Protein Analysis

135

Amino Acids

15.05

Weight (kDa)

7.61

Isoelectric Point (pI)

67.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 78 - 128 5.6e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016646)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G62890
fragaria_vesca FvH4_2g25760
malus_domestica MD11G1309500.v1.1
prunus_persica Prupe.8G261800_v2.0.a1
pyrus_communis pycom11g27060
rosa_chinensis RchiOBHm_Chr6g0294051 RchiOBHm_Chr6g0294061
rosa_laevigata RLG00000011903
rosa_multiflora Rmu_sc0016897.1_g000002
rosa_roxburghii Rroxscaffold_7G00173690
rosa_rugosa Rorug06G0240600
rosa_samantha Rh6BG360400 Rh6CG367600 Rh6DG354300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 288, 294
AccII CGCG 1 cut(s) 400
AciI CCGC 1 cut(s) 81
AcsI RAATTY 1 cut(s) 155
AfiI CCNNNNNNNGG 1 cut(s) 30
AgsI TTSAA 5 cut(s) 45, 70, 103, 111, 217
AjuI GAANNNNNNNTTGG 1 cut(s) 31
Alw26I GTCTC 1 cut(s) 39
AoxI GGCC 1 cut(s) 344
ApeKI GCWGC 1 cut(s) 200
ApoI RAATTY 1 cut(s) 155
AspLEI GCGC 3 cut(s) 56, 400, 402
AsuC2I CCSGG 1 cut(s) 31
AsuHPI GGTGA 1 cut(s) 349
BauI CACGAG 1 cut(s) 204
BbvI GCAGC 1 cut(s) 187
BccI CCATC 1 cut(s) 46
BclI TGATCA 1 cut(s) 258
BcnI CCSGG 1 cut(s) 31
BcoDI GTCTC 1 cut(s) 39
BisI GCNGC 1 cut(s) 201
BlsI GCNGC 1 cut(s) 202
Bme1390I CCNGG 1 cut(s) 31
BmrFI CCNGG 1 cut(s) 31
BmsI GCATC 3 cut(s) 160, 187, 312
BpuMI CCSGG 1 cut(s) 31
BsaI GGTCTC 1 cut(s) 39
BsaJI CCNNGG 1 cut(s) 29
Bsc4I CCNNNNNNNGG 1 cut(s) 30
BseDI CCNNGG 1 cut(s) 29
BseGI GGATG 1 cut(s) 175
BseLI CCNNNNNNNGG 1 cut(s) 30
BsePI GCGCGC 1 cut(s) 398
BseXI GCAGC 1 cut(s) 187
BsgI GTGCAG 1 cut(s) 186
Bsh1236I CGCG 1 cut(s) 400
BshFI GGCC 1 cut(s) 346
BsiSI CCGG 1 cut(s) 31
BslI CCNNNNNNNGG 1 cut(s) 30
BsmAI GTCTC 1 cut(s) 39
BsmI GAATGC 1 cut(s) 56
BsnI GGCC 1 cut(s) 346
Bso31I GGTCTC 1 cut(s) 39
Bsp143I GATC 1 cut(s) 258
BspACI CCGC 1 cut(s) 81
BspANI GGCC 1 cut(s) 346
BspFNI CGCG 1 cut(s) 400
BspTNI GGTCTC 1 cut(s) 39
BssECI CCNNGG 1 cut(s) 29
BssHII GCGCGC 1 cut(s) 398
BssMI GATC 1 cut(s) 258
BssSI CACGAG 1 cut(s) 204
Bst2BI CACGAG 1 cut(s) 204
Bst6I CTCTTC 1 cut(s) 169
BstC8I GCNNGC 1 cut(s) 400
BstF5I GGATG 1 cut(s) 175
BstFNI CGCG 1 cut(s) 400
BstHHI GCGC 3 cut(s) 56, 400, 402
BstKTI GATC 1 cut(s) 261
BstMAI GTCTC 1 cut(s) 39
BstMBI GATC 1 cut(s) 258
BstSCI CCNGG 1 cut(s) 29
BstUI CGCG 1 cut(s) 400
BstV1I GCAGC 1 cut(s) 187
BsuRI GGCC 1 cut(s) 346
BtsCI GGATG 1 cut(s) 175
Cac8I GCNNGC 1 cut(s) 400
CfoI GCGC 3 cut(s) 56, 400, 402
CviAII CATG 4 cut(s) 61, 148, 246, 361
CviJI RGCY 2 cut(s) 346, 380
CviKI_1 RGCY 2 cut(s) 346, 380
DpnI GATC 1 cut(s) 260
DpnII GATC 1 cut(s) 258
Eam1104I CTCTTC 1 cut(s) 169
EarI CTCTTC 1 cut(s) 169
Eco147I AGGCCT 1 cut(s) 346
Eco31I GGTCTC 1 cut(s) 39
FaeI CATG 4 cut(s) 64, 151, 249, 364
FatI CATG 4 cut(s) 60, 147, 245, 360
FbaI TGATCA 1 cut(s) 258
Fnu4HI GCNGC 1 cut(s) 201
FokI GGATG 1 cut(s) 182
Fsp4HI GCNGC 1 cut(s) 201
GlaI GCGC 3 cut(s) 55, 399, 401
GluI GCNGC 1 cut(s) 201
HaeIII GGCC 1 cut(s) 346
HapII CCGG 1 cut(s) 31
HhaI GCGC 3 cut(s) 56, 400, 402
Hin1II CATG 4 cut(s) 64, 151, 249, 364
Hin6I GCGC 3 cut(s) 54, 398, 400
HinP1I GCGC 3 cut(s) 54, 398, 400
HinfI GANTC 1 cut(s) 143
HpaII CCGG 1 cut(s) 31
HphI GGTGA 1 cut(s) 349
Hpy166II GTNNAC 1 cut(s) 357
Hpy188I TCNGA 1 cut(s) 169
Hpy188III TCNNGA 2 cut(s) 134, 317
Hpy8I GTNNAC 1 cut(s) 357
HpyAV CCTTC 2 cut(s) 96, 109
HpyCH4V TGCA 2 cut(s) 203, 325
Hsp92II CATG 4 cut(s) 64, 151, 249, 364
HspAI GCGC 3 cut(s) 54, 398, 400
Ksp22I TGATCA 1 cut(s) 258
Kzo9I GATC 1 cut(s) 258
LpnPI CCDG 2 cut(s) 44, 119
Lsp1109I GCAGC 1 cut(s) 187
LweI GCATC 3 cut(s) 160, 187, 312
MalI GATC 1 cut(s) 260
MboI GATC 1 cut(s) 258
MboII GAAGA 5 cut(s) 33, 99, 151, 156, 399
MluCI AATT 3 cut(s) 103, 155, 289
MmeI TCCRAC 1 cut(s) 312
MnlI CCTC 2 cut(s) 106, 336
MspI CCGG 1 cut(s) 31
MspR9I CCNGG 1 cut(s) 31
Mva1269I GAATGC 1 cut(s) 56
MvnI CGCG 1 cut(s) 400
NciI CCSGG 1 cut(s) 31
NdeII GATC 1 cut(s) 258
NlaIII CATG 4 cut(s) 64, 151, 249, 364
PauI GCGCGC 1 cut(s) 398
PceI AGGCCT 1 cut(s) 346
PctI GAATGC 1 cut(s) 56
PfeI GAWTC 1 cut(s) 143
PkrI GCNGC 1 cut(s) 202
PsiI TTATAA 2 cut(s) 288, 294
PteI GCGCGC 1 cut(s) 398
SatI GCNGC 1 cut(s) 201
Sau3AI GATC 1 cut(s) 258
ScrFI CCNGG 1 cut(s) 31
SetI ASST 1 cut(s) 88
SfaNI GCATC 3 cut(s) 160, 187, 312
Sse9I AATT 3 cut(s) 103, 155, 289
SseBI AGGCCT 1 cut(s) 346
SsiI CCGC 1 cut(s) 81
StuI AGGCCT 1 cut(s) 346
StyD4I CCNGG 1 cut(s) 29
TaqI TCGA 2 cut(s) 48, 365
TasI AATT 3 cut(s) 103, 155, 289
TfiI GAWTC 1 cut(s) 143
TseI GCWGC 1 cut(s) 200
TspDTI ATGAA 2 cut(s) 234, 234
XapI RAATTY 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.