Rh7BG171200

Methyltransferase-like protein 17

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0005103
Physical Location & Seq
Forward (+)
13345644 .. 13349068
3425 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG171200.1

Sequence Viewer

Length: 588 bp
ATGAAGGCCCTTTTCATTTTAATCGGACTGCTTTCTGCTCTGAAGAGGAAGGTGCTGAGGCTATCAGAATCCTTCACCCAAATCAAGGATGTCAACTCTCAGCTTATTGCTTCCACTTCGAAACAGCTCGTTGAGGACCCTTTGCAGTATGTGGACCAGTCACAGAGGTGGAAGATCAAGAGCTCTTTTGGAGATATTGGCCTTACTTATAGAGACGATGAGACCATTGCTTATGTTGCTTCCAGAATGCCAGCTGTCTTCTCTGCTTGTCACAGAGTACTCAAGGAGGTTCGTACAATGCTGCCAGGTTTTTCTCCTGCTAAAGTGTTGGATTTTGGTGCTGGCACGGGTTCGGCTTTCTGTGCTCTCACGATGGGGCATGTCCTTTGGAGAAAGCAGCAAAAAATACTCTTATCAAGTGCAGGGAAGAAAACGGTGGAGTACACAGTGCCATTAGCAGAGAAGACTAAAGACATGGCCATGTTTGCAGGTAAGACCACTCTGCATTATGTTGGTCATGTTGCGGTGGATTTGAAGGACAAGGCCACTGTGGTAGGTTGGACTGCAGCGCATGCACCACGCAACTGA

Protein Analysis

195

Amino Acids

21.52

Weight (kDa)

9.75

Isoelectric Point (pI)

38.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rsm22 PF09243 74 - 150 6.4e-10 Mitochondrial small ribosomal subunit Rsm22
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 479
AciI CCGC 1 cut(s) 524
AcoI YGGCCR 1 cut(s) 477
AcuI CTGAAG 1 cut(s) 62
AfaI GTAC 3 cut(s) 279, 295, 443
AfiI CCNNNNNNNGG 1 cut(s) 85
AgsI TTSAA 1 cut(s) 535
AjnI CCWGG 1 cut(s) 304
AleI CACNNNNGTG 1 cut(s) 166
AluBI AGCT 4 cut(s) 103, 127, 183, 254
AluI AGCT 4 cut(s) 103, 127, 183, 254
Alw21I GWGCWC 2 cut(s) 185, 367
Alw26I GTCTC 2 cut(s) 207, 215
AoxI GGCC 4 cut(s) 6, 199, 477, 543
ApeKI GCWGC 3 cut(s) 301, 397, 566
AspLEI GCGC 1 cut(s) 571
AspS9I GGNCC 3 cut(s) 7, 136, 154
AsuHPI GGTGA 1 cut(s) 67
AsuII TTCGAA 1 cut(s) 119
AvaII GGWCC 2 cut(s) 136, 154
BalI TGGCCA 1 cut(s) 479
BanII GRGCYC 1 cut(s) 185
BbsI GAAGAC 2 cut(s) 250, 470
Bbv12I GWGCWC 2 cut(s) 185, 367
BbvCI CCTCAGC 1 cut(s) 56
BbvI GCAGC 3 cut(s) 288, 409, 578
BccI CCATC 1 cut(s) 367
BciT130I CCWGG 1 cut(s) 306
BcoDI GTCTC 2 cut(s) 207, 215
BfmI CTRYAG 1 cut(s) 564
BfuAI ACCTGC 1 cut(s) 479
BisI GCNGC 3 cut(s) 302, 398, 567
BlsI GCNGC 3 cut(s) 303, 399, 568
BmcAI AGTACT 1 cut(s) 279
Bme1390I CCNGG 1 cut(s) 306
Bme18I GGWCC 2 cut(s) 136, 154
BmgT120I GGNCC 3 cut(s) 7, 136, 154
BmiI GGNNCC 1 cut(s) 138
BmrFI CCNGG 1 cut(s) 306
BpiI GAAGAC 2 cut(s) 250, 470
Bpu10I CCTNAGC 1 cut(s) 56
Bpu14I TTCGAA 1 cut(s) 119
BpuEI CTTGAG 1 cut(s) 266
BsaI GGTCTC 1 cut(s) 215
Bsc4I CCNNNNNNNGG 1 cut(s) 85
Bse1I ACTGG 1 cut(s) 157
Bse3DI GCAATG 1 cut(s) 225
BseBI CCWGG 1 cut(s) 306
BseGI GGATG 1 cut(s) 94
BseLI CCNNNNNNNGG 1 cut(s) 85
BseMI GCAATG 1 cut(s) 225
BseMII CTCAG 2 cut(s) 47, 113
BseNI ACTGG 1 cut(s) 157
BseXI GCAGC 3 cut(s) 288, 409, 578
BsgI GTGCAG 1 cut(s) 441
BshFI GGCC 4 cut(s) 8, 201, 479, 545
BsiHKAI GWGCWC 2 cut(s) 185, 367
BslI CCNNNNNNNGG 1 cut(s) 85
BsmAI GTCTC 2 cut(s) 207, 215
BsmBI CGTCTC 1 cut(s) 207
BsmI GAATGC 1 cut(s) 252
BsnI GGCC 4 cut(s) 8, 201, 479, 545
Bso31I GGTCTC 1 cut(s) 215
Bsp119I TTCGAA 1 cut(s) 119
Bsp1286I GDGCHC 2 cut(s) 185, 367
Bsp143I GATC 1 cut(s) 174
BspACI CCGC 1 cut(s) 524
BspANI GGCC 4 cut(s) 8, 201, 479, 545
BspCNI CTCAG 2 cut(s) 48, 112
BspLI GGNNCC 1 cut(s) 138
BspMAI CTGCAG 1 cut(s) 568
BspMI ACCTGC 1 cut(s) 479
BspT104I TTCGAA 1 cut(s) 119
BspTNI GGTCTC 1 cut(s) 215
BsrDI GCAATG 1 cut(s) 225
BsrI ACTGG 1 cut(s) 157
BssMI GATC 1 cut(s) 174
Bst2UI CCWGG 1 cut(s) 306
Bst4CI ACNGT 3 cut(s) 436, 448, 550
Bst6I CTCTTC 1 cut(s) 38
BstAPI GCANNNNNTGC 1 cut(s) 572
BstBI TTCGAA 1 cut(s) 119
BstC8I GCNNGC 3 cut(s) 252, 343, 573
BstDEI CTNAG 2 cut(s) 56, 99
BstF5I GGATG 1 cut(s) 94
BstHHI GCGC 1 cut(s) 571
BstKTI GATC 1 cut(s) 177
BstMAI GTCTC 2 cut(s) 207, 215
BstMBI GATC 1 cut(s) 174
BstMWI GCNNNNNNNGC 4 cut(s) 236, 362, 485, 572
BstNI CCWGG 1 cut(s) 306
BstNSI RCATGY 2 cut(s) 383, 575
BstSCI CCNGG 1 cut(s) 304
BstSFI CTRYAG 1 cut(s) 564
BstV1I GCAGC 3 cut(s) 288, 409, 578
BstV2I GAAGAC 2 cut(s) 250, 470
BsuRI GGCC 4 cut(s) 8, 201, 479, 545
BtsCI GGATG 1 cut(s) 94
BtsIMutI CAGTG 2 cut(s) 453, 546
BveI ACCTGC 1 cut(s) 479
Cac8I GCNNGC 3 cut(s) 252, 343, 573
CfoI GCGC 1 cut(s) 571
Cfr13I GGNCC 3 cut(s) 7, 136, 154
Csp6I GTAC 3 cut(s) 278, 294, 442
CviAII CATG 5 cut(s) 380, 475, 481, 518, 572
CviQI GTAC 3 cut(s) 278, 294, 442
DdeI CTNAG 2 cut(s) 56, 99
DpnI GATC 1 cut(s) 176
DpnII GATC 1 cut(s) 174
EaeI YGGCCR 1 cut(s) 477
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
Ecl136II GAGCTC 1 cut(s) 183
Eco24I GRGCYC 1 cut(s) 185
Eco31I GGTCTC 1 cut(s) 215
Eco47I GGWCC 2 cut(s) 136, 154
Eco53kI GAGCTC 1 cut(s) 183
Eco57I CTGAAG 1 cut(s) 62
EcoICRI GAGCTC 1 cut(s) 183
EcoO109I RGGNCCY 2 cut(s) 7, 136
EcoRII CCWGG 1 cut(s) 304
EcoT38I GRGCYC 1 cut(s) 185
Esp3I CGTCTC 1 cut(s) 207
FaeI CATG 5 cut(s) 383, 478, 484, 521, 575
FaiI YATR 9 cut(s) 150, 210, 234, 381, 476, 482, 510, 519, 573
FatI CATG 5 cut(s) 379, 474, 480, 517, 571
Fnu4HI GCNGC 3 cut(s) 302, 398, 567
FokI GGATG 1 cut(s) 101
FriOI GRGCYC 1 cut(s) 185
Fsp4HI GCNGC 3 cut(s) 302, 398, 567
GlaI GCGC 1 cut(s) 570
GluI GCNGC 3 cut(s) 302, 398, 567
HaeIII GGCC 4 cut(s) 8, 201, 479, 545
HhaI GCGC 1 cut(s) 571
Hin1II CATG 5 cut(s) 383, 478, 484, 521, 575
Hin6I GCGC 1 cut(s) 569
HinP1I GCGC 1 cut(s) 569
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HinfI GANTC 1 cut(s) 68
HphI GGTGA 1 cut(s) 67
Hpy166II GTNNAC 3 cut(s) 94, 154, 444
Hpy188I TCNGA 3 cut(s) 26, 42, 67
Hpy188III TCNNGA 3 cut(s) 178, 243, 370
Hpy8I GTNNAC 3 cut(s) 94, 154, 444
HpyAV CCTTC 3 cut(s) 43, 82, 529
HpyCH4III ACNGT 3 cut(s) 436, 448, 550
HpyCH4V TGCA 6 cut(s) 145, 422, 488, 505, 566, 575
HpyF10VI GCNNNNNNNGC 4 cut(s) 236, 362, 485, 572
HpyF3I CTNAG 2 cut(s) 56, 99
Hsp92II CATG 5 cut(s) 383, 478, 484, 521, 575
HspAI GCGC 1 cut(s) 569
Kzo9I GATC 1 cut(s) 174
LpnPI CCDG 9 cut(s) 170, 256, 264, 291, 318, 327, 330, 408, 474
Lsp1109I GCAGC 3 cut(s) 288, 409, 578
MaeIII GTNAC 2 cut(s) 159, 269
MalI GATC 1 cut(s) 176
MboI GATC 1 cut(s) 174
MboII GAAGA 5 cut(s) 55, 184, 250, 439, 475
MhlI GDGCHC 2 cut(s) 185, 367
MlsI TGGCCA 1 cut(s) 479
MluNI TGGCCA 1 cut(s) 479
MmeI TCCRAC 2 cut(s) 309, 539
MnlI CCTC 5 cut(s) 39, 51, 127, 159, 280
Mox20I TGGCCA 1 cut(s) 479
MscI TGGCCA 1 cut(s) 479
MseI TTAA 1 cut(s) 20
MslI CAYNNNNRTG 2 cut(s) 166, 479
Msp20I TGGCCA 1 cut(s) 479
MspA1I CMGCKG 1 cut(s) 254
MspR9I CCNGG 1 cut(s) 306
Mva1269I GAATGC 1 cut(s) 252
MvaI CCWGG 1 cut(s) 306
MwoI GCNNNNNNNGC 4 cut(s) 236, 362, 485, 572
NdeII GATC 1 cut(s) 174
NlaIII CATG 5 cut(s) 383, 478, 484, 521, 575
NlaIV GGNNCC 1 cut(s) 138
NmuCI GTSAC 2 cut(s) 159, 269
NspI RCATGY 2 cut(s) 383, 575
NspV TTCGAA 1 cut(s) 119
OliI CACNNNNGTG 1 cut(s) 166
PaeI GCATGC 1 cut(s) 575
PctI GAATGC 1 cut(s) 252
PfeI GAWTC 1 cut(s) 68
PkrI GCNGC 3 cut(s) 303, 399, 568
PpuMI RGGWCCY 1 cut(s) 136
Psp124BI GAGCTC 1 cut(s) 185
Psp5II RGGWCCY 1 cut(s) 136
Psp6I CCWGG 1 cut(s) 304
PspGI CCWGG 1 cut(s) 304
PspN4I GGNNCC 1 cut(s) 138
PspPI GGNCC 3 cut(s) 7, 136, 154
PspPPI RGGWCCY 1 cut(s) 136
PstI CTGCAG 1 cut(s) 568
PvuII CAGCTG 1 cut(s) 254
RsaI GTAC 3 cut(s) 279, 295, 443
RsaNI GTAC 3 cut(s) 278, 294, 442
RseI CAYNNNNRTG 2 cut(s) 166, 479
SacI GAGCTC 1 cut(s) 185
SaqAI TTAA 1 cut(s) 20
SatI GCNGC 3 cut(s) 302, 398, 567
Sau3AI GATC 1 cut(s) 174
Sau96I GGNCC 3 cut(s) 7, 136, 154
ScaI AGTACT 1 cut(s) 279
ScrFI CCNGG 1 cut(s) 306
SduI GDGCHC 2 cut(s) 185, 367
SfcI CTRYAG 1 cut(s) 564
SfuI TTCGAA 1 cut(s) 119
SinI GGWCC 2 cut(s) 136, 154
SmiMI CAYNNNNRTG 2 cut(s) 166, 479
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
SphI GCATGC 1 cut(s) 575
SsiI CCGC 1 cut(s) 524
SstI GAGCTC 1 cut(s) 185
StyD4I CCNGG 1 cut(s) 304
TaaI ACNGT 3 cut(s) 436, 448, 550
TaqI TCGA 1 cut(s) 119
TatI WGTACW 2 cut(s) 277, 441
TfiI GAWTC 1 cut(s) 68
Tru1I TTAA 1 cut(s) 20
Tru9I TTAA 1 cut(s) 20
TscAI CASTG 2 cut(s) 453, 553
TseFI GTSAC 2 cut(s) 159, 269
TseI GCWGC 3 cut(s) 301, 397, 566
Tsp45I GTSAC 2 cut(s) 159, 269
TspDTI ATGAA 2 cut(s) 4, 17
TspRI CASTG 2 cut(s) 453, 553
VpaK11BI GGWCC 2 cut(s) 136, 154
XceI RCATGY 2 cut(s) 383, 575
ZrmI AGTACT 1 cut(s) 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.