Rh7BG243100
HSP70 Family

Belongs to the heat shock protein 70 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0017449
Physical Location & Seq
Reverse (-)
21656021 .. 21656646
626 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG243100.1

Sequence Viewer

Length: 600 bp
ATGGACGCGGCCACAATTGCCGGCTTGAAGCCTCTGTGCTTGTTTCATGAAACTAAAGCGTCTGCTTTGGAGTTTGGTGTTTACAAGAAGGATTTACCAGAAAGTAAACACAGCCAACTCAATGTTGCTTTTGTGGATATTGGACATGCTAGCTCGCAAGTTTGCATTGCTACCTTTAAGAAGGGCGTGATGAAAATACTAGTTCATTCATTTGACCAGTGTTTGGGTGGTAGGGTTTTTGATGTACTATTCCGGCATTTCTCTGCGAAATTTCAGGACGAGTACCAGATCAATGTTTTTCAGAACACCAGGGCCTGCCATCAGCTTCAAACTGCATGTGACTGCACGTACATTCAAATATCTACATCAGGTGAACGGGTGAAAATGAAGGTGAAAGTTCGCTTGAATTTGAATGATATTGTGTCTATAGAGTCGGCAACTGTTTTGGAAAAGAAAGAAGTTCATGGGGTTCTAGAGGTAACAGAAGTTCGTGTGCCTGTGGCCGAATTAGTTTATGGAGGAATGACTGAAGCAGAAGTGCATAAGGCAAGGAAGAAGGAGTTTGAGATGGCTTCACAAGATCGATTAATGGAGACCTGA

Protein Analysis

199

Amino Acids

22.34

Weight (kDa)

7.08

Isoelectric Point (pI)

36.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 1 - 123 5.3e-24 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 223
AccII CGCG 1 cut(s) 8
AciI CCGC 1 cut(s) 8
AcoI YGGCCR 2 cut(s) 9, 501
AcsI RAATTY 2 cut(s) 269, 406
AcuI CTGAAG 1 cut(s) 549
AfaI GTAC 3 cut(s) 246, 284, 350
AfiI CCNNNNNNNGG 1 cut(s) 223
AgsI TTSAA 5 cut(s) 28, 329, 356, 406, 412
AhlI ACTAGT 1 cut(s) 199
AjnI CCWGG 1 cut(s) 308
AluBI AGCT 2 cut(s) 153, 325
AluI AGCT 2 cut(s) 153, 325
Alw26I GTCTC 1 cut(s) 587
AlwNI CAGNNNCTG 1 cut(s) 315
AoxI GGCC 3 cut(s) 9, 312, 501
ApoI RAATTY 2 cut(s) 269, 406
AseI ATTAAT 1 cut(s) 587
AspS9I GGNCC 1 cut(s) 312
AsuHPI GGTGA 3 cut(s) 383, 391, 403
AsuNHI GCTAGC 1 cut(s) 149
BccI CCATC 2 cut(s) 327, 562
BciT130I CCWGG 1 cut(s) 310
BcoDI GTCTC 1 cut(s) 587
BcuI ACTAGT 1 cut(s) 199
BfaI CTAG 3 cut(s) 150, 200, 473
BfmI CTRYAG 1 cut(s) 426
BisI GCNGC 1 cut(s) 9
BlsI GCNGC 1 cut(s) 10
Bme1390I CCNGG 1 cut(s) 310
BmgT120I GGNCC 1 cut(s) 312
BmrFI CCNGG 1 cut(s) 310
BmtI GCTAGC 1 cut(s) 153
Bsa29I ATCGAT 1 cut(s) 583
BsaAI YACGTR 1 cut(s) 348
BsaI GGTCTC 1 cut(s) 587
BsaJI CCNNGG 1 cut(s) 309
Bsc4I CCNNNNNNNGG 1 cut(s) 223
Bse118I RCCGGY 1 cut(s) 20
Bse1I ACTGG 1 cut(s) 217
Bse3DI GCAATG 1 cut(s) 165
BseBI CCWGG 1 cut(s) 310
BseCI ATCGAT 1 cut(s) 583
BseDI CCNNGG 1 cut(s) 309
BseLI CCNNNNNNNGG 1 cut(s) 223
BseMI GCAATG 1 cut(s) 165
BseNI ACTGG 1 cut(s) 217
BsgI GTGCAG 1 cut(s) 328
Bsh1236I CGCG 1 cut(s) 8
BshFI GGCC 3 cut(s) 11, 314, 503
BshVI ATCGAT 1 cut(s) 583
BsiSI CCGG 2 cut(s) 21, 253
BslI CCNNNNNNNGG 1 cut(s) 223
BsmAI GTCTC 1 cut(s) 587
BsnI GGCC 3 cut(s) 11, 314, 503
Bso31I GGTCTC 1 cut(s) 587
Bsp143I GATC 2 cut(s) 288, 580
BspACI CCGC 1 cut(s) 8
BspANI GGCC 3 cut(s) 11, 314, 503
BspDI ATCGAT 1 cut(s) 583
BspFNI CGCG 1 cut(s) 8
BspHI TCATGA 1 cut(s) 46
BspOI GCTAGC 1 cut(s) 153
BspTNI GGTCTC 1 cut(s) 587
BsrDI GCAATG 1 cut(s) 165
BsrFI RCCGGY 1 cut(s) 20
BsrI ACTGG 1 cut(s) 217
BssAI RCCGGY 1 cut(s) 20
BssECI CCNNGG 1 cut(s) 309
BssMI GATC 2 cut(s) 288, 580
Bst2UI CCWGG 1 cut(s) 310
Bst4CI ACNGT 1 cut(s) 442
BstBAI YACGTR 1 cut(s) 348
BstC8I GCNNGC 4 cut(s) 22, 151, 155, 316
BstFNI CGCG 1 cut(s) 8
BstKTI GATC 2 cut(s) 291, 583
BstMAI GTCTC 1 cut(s) 587
BstMBI GATC 2 cut(s) 288, 580
BstMWI GCNNNNNNNGC 1 cut(s) 17
BstNI CCWGG 1 cut(s) 310
BstNSI RCATGY 2 cut(s) 149, 339
BstSCI CCNGG 1 cut(s) 308
BstSFI CTRYAG 1 cut(s) 426
BstUI CGCG 1 cut(s) 8
Bsu15I ATCGAT 1 cut(s) 583
BsuRI GGCC 3 cut(s) 11, 314, 503
BsuTUI ATCGAT 1 cut(s) 583
BtsIMutI CAGTG 1 cut(s) 224
Cac8I GCNNGC 4 cut(s) 22, 151, 155, 316
CaiI CAGNNNCTG 1 cut(s) 315
CciI TCATGA 1 cut(s) 46
Cfr10I RCCGGY 1 cut(s) 20
Cfr13I GGNCC 1 cut(s) 312
ClaI ATCGAT 1 cut(s) 583
CseI GACGC 2 cut(s) 14, 48
Csp6I GTAC 3 cut(s) 245, 283, 349
CviAII CATG 4 cut(s) 47, 146, 336, 464
CviJI RGCY 9 cut(s) 11, 24, 31, 114, 153, 314, 325, 503, 572
CviKI_1 RGCY 9 cut(s) 11, 24, 31, 114, 153, 314, 325, 503, 572
CviQI GTAC 3 cut(s) 245, 283, 349
DpnI GATC 2 cut(s) 290, 582
DpnII GATC 2 cut(s) 288, 580
EaeI YGGCCR 2 cut(s) 9, 501
Eco31I GGTCTC 1 cut(s) 587
Eco57I CTGAAG 1 cut(s) 549
EcoO109I RGGNCCY 1 cut(s) 312
EcoRII CCWGG 1 cut(s) 308
FaeI CATG 4 cut(s) 50, 149, 339, 467
FaiI YATR 7 cut(s) 48, 147, 337, 428, 465, 516, 543
FatI CATG 4 cut(s) 46, 145, 335, 463
Fnu4HI GCNGC 1 cut(s) 9
Fsp4HI GCNGC 1 cut(s) 9
FspBI CTAG 3 cut(s) 150, 200, 473
GluI GCNGC 1 cut(s) 9
HaeIII GGCC 3 cut(s) 11, 314, 503
HapII CCGG 2 cut(s) 21, 253
HgaI GACGC 2 cut(s) 14, 48
Hin1II CATG 4 cut(s) 50, 149, 339, 467
HinfI GANTC 1 cut(s) 431
HpaII CCGG 2 cut(s) 21, 253
HphI GGTGA 3 cut(s) 383, 391, 403
Hpy166II GTNNAC 3 cut(s) 82, 107, 374
Hpy188I TCNGA 1 cut(s) 303
Hpy188III TCNNGA 3 cut(s) 47, 275, 473
Hpy8I GTNNAC 3 cut(s) 82, 107, 374
HpyAV CCTTC 4 cut(s) 82, 175, 382, 550
HpyCH4III ACNGT 1 cut(s) 442
HpyCH4IV ACGT 1 cut(s) 347
HpyCH4V TGCA 4 cut(s) 165, 335, 345, 541
HpyF10VI GCNNNNNNNGC 1 cut(s) 17
HpySE526I ACGT 1 cut(s) 347
Hsp92II CATG 4 cut(s) 50, 149, 339, 467
KroI GCCGGC 1 cut(s) 20
KroNI GCCGGC 1 cut(s) 22
Kzo9I GATC 2 cut(s) 288, 580
MaeI CTAG 3 cut(s) 150, 200, 473
MaeII ACGT 1 cut(s) 347
MaeIII GTNAC 2 cut(s) 338, 478
MalI GATC 2 cut(s) 290, 582
MboI GATC 2 cut(s) 288, 580
MboII GAAGA 1 cut(s) 565
MfeI CAATTG 1 cut(s) 15
MluCI AATT 4 cut(s) 15, 269, 406, 506
MlyI GAGTC 1 cut(s) 440
MnlI CCTC 3 cut(s) 42, 469, 512
MroNI GCCGGC 1 cut(s) 20
MseI TTAA 2 cut(s) 177, 587
MspI CCGG 2 cut(s) 21, 253
MspR9I CCNGG 1 cut(s) 310
MunI CAATTG 1 cut(s) 15
MvaI CCWGG 1 cut(s) 310
MvnI CGCG 1 cut(s) 8
MwoI GCNNNNNNNGC 1 cut(s) 17
NaeI GCCGGC 1 cut(s) 22
NdeII GATC 2 cut(s) 288, 580
NgoMIV GCCGGC 1 cut(s) 20
NheI GCTAGC 1 cut(s) 149
NlaIII CATG 4 cut(s) 50, 149, 339, 467
NmuCI GTSAC 1 cut(s) 338
NspI RCATGY 2 cut(s) 149, 339
PagI TCATGA 1 cut(s) 46
PdiI GCCGGC 1 cut(s) 22
PflMI CCANNNNNTGG 1 cut(s) 223
PkrI GCNGC 1 cut(s) 10
PleI GAGTC 1 cut(s) 439
PpsI GAGTC 1 cut(s) 439
Ppu21I YACGTR 1 cut(s) 348
PshBI ATTAAT 1 cut(s) 587
Psp6I CCWGG 1 cut(s) 308
PspGI CCWGG 1 cut(s) 308
PspPI GGNCC 1 cut(s) 312
PsrI GAACNNNNNNTAC 2 cut(s) 471, 503
PstNI CAGNNNCTG 1 cut(s) 315
RsaI GTAC 3 cut(s) 246, 284, 350
RsaNI GTAC 3 cut(s) 245, 283, 349
SaqAI TTAA 2 cut(s) 177, 587
SatI GCNGC 1 cut(s) 9
Sau3AI GATC 2 cut(s) 288, 580
Sau96I GGNCC 1 cut(s) 312
SchI GAGTC 1 cut(s) 440
ScrFI CCNGG 1 cut(s) 310
SetI ASST 8 cut(s) 155, 176, 327, 350, 373, 393, 480, 599
SfcI CTRYAG 1 cut(s) 426
SpeI ACTAGT 1 cut(s) 199
Sse9I AATT 4 cut(s) 15, 269, 406, 506
SsiI CCGC 1 cut(s) 8
SspMI CTAG 3 cut(s) 150, 200, 473
StyD4I CCNGG 1 cut(s) 308
TaaI ACNGT 1 cut(s) 442
TaiI ACGT 1 cut(s) 350
TaqI TCGA 1 cut(s) 583
TasI AATT 4 cut(s) 15, 269, 406, 506
TatI WGTACW 1 cut(s) 244
TauI GCSGC 1 cut(s) 11
Tru1I TTAA 2 cut(s) 177, 587
Tru9I TTAA 2 cut(s) 177, 587
TscAI CASTG 1 cut(s) 224
TseFI GTSAC 1 cut(s) 338
Tsp45I GTSAC 1 cut(s) 338
TspDTI ATGAA 7 cut(s) 35, 63, 194, 198, 206, 401, 452
TspRI CASTG 1 cut(s) 224
Van91I CCANNNNNTGG 1 cut(s) 223
VspI ATTAAT 1 cut(s) 587
XapI RAATTY 2 cut(s) 269, 406
XbaI TCTAGA 1 cut(s) 472
XceI RCATGY 2 cut(s) 149, 339
XcmI CCANNNNNNNNNTGG 1 cut(s) 224
XspI CTAG 3 cut(s) 150, 200, 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.