Rw0G020030

E3 ubiquitin-protein ligase RNF115-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00903
OG0017317
Physical Location & Seq
Reverse (-)
7371 .. 10301
2931 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G020030.1

Sequence Viewer

Length: 714 bp
ATGGATGAGCAGTGTGATTCCAGGATGTTTAACTGGTGTGAAGTGATGCAAACAGAAGTACATGAGGGACCCTATCCGCCAATATCACAATGCTACGACTTGGTCATCAGATTAACAGTCATCATGCAAGCGGGGGCTTTTATGATAGACACCAATCCACCACATTCTTTTATTTATAATTTTGATCAGGAACCATGTATATTAGGGGAAACAAAGATTTGGGTCAACTTCACCTCTGTAGAGAAACAACATCAGAGCCTGAAGGACTTGATGTTTGCGCTTTGTTTCATGGGCGTTGAGCAAAAAGACTGCGGAGATATTGCTGTAGAAATATTAAACAAAGTTCAACCGGCCATCCCCCTTCCCCAGTTGGCTATGGATGTGACCGTAAGGCTCACGTATTCCGAATTGTATGGAAGATTTGACGGTTATGATGATATGCGCTTGCATCGCTATGTAAAGAAATCATGGGACGACTTTAGGGCTGCTACTACCTCCTGCACTGGTTCATGTCCTGTTTGTTTGGAGGATTTTGGAGGTGGAGTTGATGCTAAAATGATTGCTCGTTTGCCGTGCTTACATTATTTTCATGCAGCTTGCACTGCCCAGTGGATTAATATCCGTCGCACGTGTCCCGTGTGCCGAACCCAAATGATGCCACTTGTATGTGATGGAAATGAACCCTTCAATCCTAACCCCATCTGGCCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

237

Amino Acids

27.09

Weight (kDa)

4.98

Isoelectric Point (pI)

48.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 170 - 215 3.6e-12 Ring finger domain
zf-C3HC4_2 PF13923 171 - 214 1.2e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 171 - 214 2.2e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 171 - 212 5.8e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017317)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g00591 FvH4_6g00620
rosa_chinensis RchiOBHm_Chr3g0447991 RchiOBHm_Chr3g0448031
rosa_laevigata RLG00000025954
rosa_multiflora Rmu_sc0005969.1_g000012 Rmu_sc0005969.1_g000016
rosa_roxburghii Rroxscaffold_6G00426400
rosa_samantha Rh3AG007300 Rh3CG007100 Rh3DG007500
rosa_wichuraiana Rw0G020030 Rw3G000570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 177
AciI CCGC 3 cut(s) 77, 131, 312
AcoI YGGCCR 1 cut(s) 351
AcuI CTGAAG 1 cut(s) 281
AcvI CACGTG 1 cut(s) 630
AfaI GTAC 1 cut(s) 60
AflIII ACRYGT 1 cut(s) 629
AgsI TTSAA 2 cut(s) 347, 688
AjnI CCWGG 1 cut(s) 20
AluBI AGCT 1 cut(s) 596
AluI AGCT 1 cut(s) 596
AlwNI CAGNNNCTG 1 cut(s) 259
AoxI GGCC 2 cut(s) 351, 704
ApeKI GCWGC 2 cut(s) 485, 593
ArsI GACNNNNNNTTYG 2 cut(s) 257, 289
AseI ATTAAT 1 cut(s) 615
AspLEI GCGC 2 cut(s) 280, 444
AspS9I GGNCC 1 cut(s) 68
AsuHPI GGTGA 1 cut(s) 223
AvaII GGWCC 1 cut(s) 68
BbrPI CACGTG 1 cut(s) 630
BbvI GCAGC 2 cut(s) 472, 605
BccI CCATC 3 cut(s) 362, 665, 707
BceAI ACGGC 1 cut(s) 556
BciT130I CCWGG 1 cut(s) 22
BclI TGATCA 1 cut(s) 184
BfmI CTRYAG 2 cut(s) 237, 324
BisI GCNGC 2 cut(s) 486, 594
BlsI GCNGC 2 cut(s) 487, 595
Bme1390I CCNGG 1 cut(s) 22
Bme18I GGWCC 1 cut(s) 68
BmgT120I GGNCC 1 cut(s) 68
BmiI GGNNCC 3 cut(s) 69, 70, 192
BmrFI CCNGG 1 cut(s) 22
BmrI ACTGGG 2 cut(s) 361, 601
BmsI GCATC 4 cut(s) 36, 457, 538, 645
BmuI ACTGGG 2 cut(s) 361, 601
BsaAI YACGTR 2 cut(s) 399, 630
BsaBI GATNNNNATC 1 cut(s) 617
BsaXI ACNNNNNCTCC 2 cut(s) 528, 558
Bse118I RCCGGY 1 cut(s) 349
Bse1I ACTGG 4 cut(s) 38, 367, 508, 607
Bse8I GATNNNNATC 1 cut(s) 617
BseBI CCWGG 1 cut(s) 22
BseGI GGATG 4 cut(s) 10, 30, 354, 385
BseJI GATNNNNATC 1 cut(s) 617
BseNI ACTGG 4 cut(s) 38, 367, 508, 607
BseXI GCAGC 2 cut(s) 472, 605
BsgI GTGCAG 1 cut(s) 484
BshFI GGCC 2 cut(s) 353, 706
BsiSI CCGG 1 cut(s) 350
BslFI GGGAC 3 cut(s) 81, 485, 618
BsmFI GGGAC 3 cut(s) 81, 485, 618
BsnI GGCC 2 cut(s) 353, 706
Bsp143I GATC 1 cut(s) 184
BspACI CCGC 3 cut(s) 77, 131, 312
BspANI GGCC 2 cut(s) 353, 706
BspLI GGNNCC 3 cut(s) 69, 70, 192
BsrFI RCCGGY 1 cut(s) 349
BsrI ACTGG 4 cut(s) 38, 367, 508, 607
BssAI RCCGGY 1 cut(s) 349
BssMI GATC 1 cut(s) 184
Bst2UI CCWGG 1 cut(s) 22
Bst4CI ACNGT 3 cut(s) 118, 388, 428
BstBAI YACGTR 2 cut(s) 399, 630
BstC8I GCNNGC 4 cut(s) 129, 446, 598, 708
BstF5I GGATG 4 cut(s) 10, 30, 354, 385
BstHHI GCGC 2 cut(s) 280, 444
BstKTI GATC 1 cut(s) 187
BstMBI GATC 1 cut(s) 184
BstMWI GCNNNNNNNGC 2 cut(s) 450, 602
BstNI CCWGG 1 cut(s) 22
BstSCI CCNGG 1 cut(s) 20
BstSFI CTRYAG 2 cut(s) 237, 324
BstV1I GCAGC 2 cut(s) 472, 605
BsuRI GGCC 2 cut(s) 353, 706
BtgZI GCGATG 1 cut(s) 434
BtsCI GGATG 4 cut(s) 10, 30, 354, 385
BtsI GCAGTG 2 cut(s) 17, 600
BtsIMutI CAGTG 4 cut(s) 17, 501, 600, 614
Cac8I GCNNGC 4 cut(s) 129, 446, 598, 708
CaiI CAGNNNCTG 1 cut(s) 259
CfoI GCGC 2 cut(s) 280, 444
Cfr10I RCCGGY 1 cut(s) 349
Cfr13I GGNCC 1 cut(s) 68
Csp6I GTAC 1 cut(s) 59
CviAII CATG 8 cut(s) 62, 124, 195, 289, 468, 510, 590, 711
CviJI RGCY 8 cut(s) 137, 258, 353, 374, 394, 485, 596, 706
CviKI_1 RGCY 8 cut(s) 137, 258, 353, 374, 394, 485, 596, 706
CviQI GTAC 1 cut(s) 59
DpnI GATC 1 cut(s) 186
DpnII GATC 1 cut(s) 184
EaeI YGGCCR 1 cut(s) 351
EciI GGCGGA 1 cut(s) 66
Eco47I GGWCC 1 cut(s) 68
Eco57I CTGAAG 1 cut(s) 281
Eco72I CACGTG 1 cut(s) 630
EcoO109I RGGNCCY 1 cut(s) 68
EcoRII CCWGG 1 cut(s) 20
FaeI CATG 8 cut(s) 65, 127, 198, 292, 471, 513, 593, 714
FaqI GGGAC 3 cut(s) 81, 485, 618
FatI CATG 8 cut(s) 61, 123, 194, 288, 467, 509, 589, 710
FauI CCCGC 1 cut(s) 124
FbaI TGATCA 1 cut(s) 184
Fnu4HI GCNGC 2 cut(s) 486, 594
FokI GGATG 4 cut(s) 17, 37, 341, 392
Fsp4HI GCNGC 2 cut(s) 486, 594
GlaI GCGC 2 cut(s) 279, 443
GluI GCNGC 2 cut(s) 486, 594
HaeIII GGCC 2 cut(s) 353, 706
HapII CCGG 1 cut(s) 350
HhaI GCGC 2 cut(s) 280, 444
Hin1II CATG 8 cut(s) 65, 127, 198, 292, 471, 513, 593, 714
Hin6I GCGC 2 cut(s) 278, 442
HinP1I GCGC 2 cut(s) 278, 442
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HinfI GANTC 1 cut(s) 17
HpaII CCGG 1 cut(s) 350
HphI GGTGA 1 cut(s) 223
Hpy166II GTNNAC 1 cut(s) 226
Hpy188I TCNGA 3 cut(s) 110, 255, 406
Hpy188III TCNNGA 1 cut(s) 188
Hpy8I GTNNAC 1 cut(s) 226
Hpy99I CGWCG 1 cut(s) 627
HpyAV CCTTC 3 cut(s) 256, 371, 694
HpyCH4III ACNGT 3 cut(s) 118, 388, 428
HpyCH4IV ACGT 2 cut(s) 398, 629
HpyCH4V TGCA 7 cut(s) 49, 127, 448, 501, 593, 600, 710
HpyF10VI GCNNNNNNNGC 2 cut(s) 450, 602
HpySE526I ACGT 2 cut(s) 398, 629
Hsp92II CATG 8 cut(s) 65, 127, 198, 292, 471, 513, 593, 714
HspAI GCGC 2 cut(s) 278, 442
KflI GGGWCCC 1 cut(s) 68
Ksp22I TGATCA 1 cut(s) 184
Kzo9I GATC 1 cut(s) 184
Lsp1109I GCAGC 2 cut(s) 472, 605
LweI GCATC 4 cut(s) 36, 457, 538, 645
MaeII ACGT 2 cut(s) 398, 629
MaeIII GTNAC 1 cut(s) 382
MalI GATC 1 cut(s) 186
MboI GATC 1 cut(s) 184
MboII GAAGA 1 cut(s) 429
MluCI AATT 2 cut(s) 178, 407
MnlI CCTC 5 cut(s) 58, 244, 505, 520, 530
MseI TTAA 4 cut(s) 30, 113, 335, 615
MslI CAYNNNNRTG 2 cut(s) 453, 664
MspI CCGG 1 cut(s) 350
MspR9I CCNGG 1 cut(s) 22
MvaI CCWGG 1 cut(s) 22
MwoI GCNNNNNNNGC 2 cut(s) 450, 602
NdeII GATC 1 cut(s) 184
NlaIII CATG 8 cut(s) 65, 127, 198, 292, 471, 513, 593, 714
NlaIV GGNNCC 3 cut(s) 69, 70, 192
NmuCI GTSAC 1 cut(s) 382
PfeI GAWTC 1 cut(s) 17
PflFI GACNNNGTC 1 cut(s) 101
PfoI TCCNGGA 1 cut(s) 20
PkrI GCNGC 2 cut(s) 487, 595
PmaCI CACGTG 1 cut(s) 630
PmlI CACGTG 1 cut(s) 630
Ppu21I YACGTR 2 cut(s) 399, 630
PpuMI RGGWCCY 1 cut(s) 68
PshBI ATTAAT 1 cut(s) 615
PsiI TTATAA 1 cut(s) 177
Psp5II RGGWCCY 1 cut(s) 68
Psp6I CCWGG 1 cut(s) 20
PspCI CACGTG 1 cut(s) 630
PspGI CCWGG 1 cut(s) 20
PspN4I GGNNCC 3 cut(s) 69, 70, 192
PspPI GGNCC 1 cut(s) 68
PspPPI RGGWCCY 1 cut(s) 68
PstNI CAGNNNCTG 1 cut(s) 259
PsyI GACNNNGTC 1 cut(s) 101
RsaI GTAC 1 cut(s) 60
RsaNI GTAC 1 cut(s) 59
RseI CAYNNNNRTG 2 cut(s) 453, 664
SaqAI TTAA 4 cut(s) 30, 113, 335, 615
SatI GCNGC 2 cut(s) 486, 594
Sau3AI GATC 1 cut(s) 184
Sau96I GGNCC 1 cut(s) 68
ScrFI CCNGG 1 cut(s) 22
SetI ASST 6 cut(s) 236, 401, 497, 541, 598, 632
SfaNI GCATC 4 cut(s) 36, 457, 538, 645
SfcI CTRYAG 2 cut(s) 237, 324
SinI GGWCC 1 cut(s) 68
SmiMI CAYNNNNRTG 2 cut(s) 453, 664
Sse9I AATT 2 cut(s) 178, 407
SsiI CCGC 3 cut(s) 77, 131, 312
SspI AATATT 1 cut(s) 333
StyD4I CCNGG 1 cut(s) 20
TaaI ACNGT 3 cut(s) 118, 388, 428
TaiI ACGT 2 cut(s) 401, 632
TasI AATT 2 cut(s) 178, 407
TatI WGTACW 1 cut(s) 58
TfiI GAWTC 1 cut(s) 17
Tru1I TTAA 4 cut(s) 30, 113, 335, 615
Tru9I TTAA 4 cut(s) 30, 113, 335, 615
TscAI CASTG 4 cut(s) 17, 508, 607, 614
TseFI GTSAC 1 cut(s) 382
TseI GCWGC 2 cut(s) 485, 593
Tsp45I GTSAC 1 cut(s) 382
TspDTI ATGAA 4 cut(s) 277, 498, 578, 693
TspGWI ACGGA 1 cut(s) 611
TspRI CASTG 4 cut(s) 17, 508, 607, 614
Tth111I GACNNNGTC 1 cut(s) 101
VpaK11BI GGWCC 1 cut(s) 68
VspI ATTAAT 1 cut(s) 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.