Rw3G000570

E3 ubiquitin-protein ligase RNF115-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0017317
Physical Location & Seq
Reverse (-)
468891 .. 469334
444 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G000570.1

Sequence Viewer

Length: 444 bp
ATGTTTGCGCTTTGTTTCATGGGCGTTGAGCAAAAAGACTGCGGAGATATTGCTCTAGAAATATTAAACAAAGTTCAACCGGCCATCCCCCTTCCCCAGTTGGCTATGGATGTGATCGTAAGGCTCACGTATTCTGAATTGTATGGAAGATTTGACGGTTATGATGATATGCGCTTGCATCGCTATGTAAAGAAATCATGGGACGACTTTAGGGCTGCTACTACCTCCTGCACTGGTTCATGTCCTGTTTGTTTGGAGGATTTTGGAGGTGGAGTTGATGCTAAAATGATTGCTCGTTTGCCCTGCTTACATTATTTTCATGCAGCTTGCACTGCCCAGTGGGTTAATATCCGTCGCACGTGTCCCGTGTGCCGAACCCAAATGCTGCCACTTGTATGTGATGGAAATGAACCCTTCAATCCTAACCCCATCTGGCCTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

147

Amino Acids

16.6

Weight (kDa)

5.86

Isoelectric Point (pI)

58.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 80 - 125 1.3e-12 Ring finger domain
zf-C3HC4_2 PF13923 81 - 124 8e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 81 - 122 2.9e-06 RING-type zinc-finger
zf-C3HC4 PF00097 81 - 124 1.9e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017317)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g00591 FvH4_6g00620
rosa_chinensis RchiOBHm_Chr3g0447991 RchiOBHm_Chr3g0448031
rosa_laevigata RLG00000025954
rosa_multiflora Rmu_sc0005969.1_g000012 Rmu_sc0005969.1_g000016
rosa_roxburghii Rroxscaffold_6G00426400
rosa_samantha Rh3AG007300 Rh3CG007100 Rh3DG007500
rosa_wichuraiana Rw0G020030 Rw3G000570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 42
AcoI YGGCCR 1 cut(s) 81
AcvI CACGTG 1 cut(s) 360
AflIII ACRYGT 1 cut(s) 359
AgsI TTSAA 2 cut(s) 77, 418
AluBI AGCT 1 cut(s) 326
AluI AGCT 1 cut(s) 326
AoxI GGCC 2 cut(s) 81, 434
ApeKI GCWGC 3 cut(s) 215, 323, 385
AspLEI GCGC 2 cut(s) 10, 174
BbrPI CACGTG 1 cut(s) 360
BbvI GCAGC 3 cut(s) 202, 335, 372
BccI CCATC 3 cut(s) 92, 395, 437
BfaI CTAG 1 cut(s) 56
BisI GCNGC 3 cut(s) 216, 324, 386
BlsI GCNGC 3 cut(s) 217, 325, 387
BmrI ACTGGG 2 cut(s) 91, 331
BmsI GCATC 2 cut(s) 187, 268
BmuI ACTGGG 2 cut(s) 91, 331
BsaAI YACGTR 2 cut(s) 129, 360
BsaXI ACNNNNNCTCC 2 cut(s) 258, 288
Bse118I RCCGGY 1 cut(s) 79
Bse1I ACTGG 3 cut(s) 97, 238, 337
BseGI GGATG 2 cut(s) 84, 115
BseNI ACTGG 3 cut(s) 97, 238, 337
BseXI GCAGC 3 cut(s) 202, 335, 372
BsgI GTGCAG 1 cut(s) 214
BshFI GGCC 2 cut(s) 83, 436
BsiSI CCGG 1 cut(s) 80
BslFI GGGAC 2 cut(s) 215, 348
BsmFI GGGAC 2 cut(s) 215, 348
BsnI GGCC 2 cut(s) 83, 436
Bsp143I GATC 1 cut(s) 114
BspACI CCGC 1 cut(s) 42
BspANI GGCC 2 cut(s) 83, 436
BsrFI RCCGGY 1 cut(s) 79
BsrI ACTGG 3 cut(s) 97, 238, 337
BssAI RCCGGY 1 cut(s) 79
BssMI GATC 1 cut(s) 114
Bst4CI ACNGT 1 cut(s) 158
BstBAI YACGTR 2 cut(s) 129, 360
BstC8I GCNNGC 2 cut(s) 176, 328
BstF5I GGATG 2 cut(s) 84, 115
BstHHI GCGC 2 cut(s) 10, 174
BstKTI GATC 1 cut(s) 117
BstMBI GATC 1 cut(s) 114
BstMWI GCNNNNNNNGC 2 cut(s) 180, 332
BstV1I GCAGC 3 cut(s) 202, 335, 372
BsuRI GGCC 2 cut(s) 83, 436
BtgZI GCGATG 1 cut(s) 164
BtsCI GGATG 2 cut(s) 84, 115
BtsI GCAGTG 1 cut(s) 330
BtsIMutI CAGTG 3 cut(s) 231, 330, 344
Cac8I GCNNGC 2 cut(s) 176, 328
CfoI GCGC 2 cut(s) 10, 174
Cfr10I RCCGGY 1 cut(s) 79
CviAII CATG 4 cut(s) 19, 198, 240, 320
CviJI RGCY 6 cut(s) 83, 104, 124, 215, 326, 436
CviKI_1 RGCY 6 cut(s) 83, 104, 124, 215, 326, 436
DpnI GATC 1 cut(s) 116
DpnII GATC 1 cut(s) 114
EaeI YGGCCR 1 cut(s) 81
Eco72I CACGTG 1 cut(s) 360
FaeI CATG 4 cut(s) 22, 201, 243, 323
FaqI GGGAC 2 cut(s) 215, 348
FatI CATG 4 cut(s) 18, 197, 239, 319
Fnu4HI GCNGC 3 cut(s) 216, 324, 386
FokI GGATG 2 cut(s) 71, 122
Fsp4HI GCNGC 3 cut(s) 216, 324, 386
FspBI CTAG 1 cut(s) 56
GlaI GCGC 2 cut(s) 9, 173
GluI GCNGC 3 cut(s) 216, 324, 386
HaeIII GGCC 2 cut(s) 83, 436
HapII CCGG 1 cut(s) 80
HhaI GCGC 2 cut(s) 10, 174
Hin1II CATG 4 cut(s) 22, 201, 243, 323
Hin6I GCGC 2 cut(s) 8, 172
HinP1I GCGC 2 cut(s) 8, 172
HpaII CCGG 1 cut(s) 80
Hpy188I TCNGA 1 cut(s) 136
Hpy188III TCNNGA 1 cut(s) 56
Hpy99I CGWCG 1 cut(s) 357
HpyAV CCTTC 2 cut(s) 101, 424
HpyCH4III ACNGT 1 cut(s) 158
HpyCH4IV ACGT 2 cut(s) 128, 359
HpyCH4V TGCA 4 cut(s) 178, 231, 323, 330
HpyF10VI GCNNNNNNNGC 2 cut(s) 180, 332
HpySE526I ACGT 2 cut(s) 128, 359
Hsp92II CATG 4 cut(s) 22, 201, 243, 323
HspAI GCGC 2 cut(s) 8, 172
Kzo9I GATC 1 cut(s) 114
LpnPI CCDG 8 cut(s) 93, 110, 219, 241, 258, 316, 350, 418
Lsp1109I GCAGC 3 cut(s) 202, 335, 372
LweI GCATC 2 cut(s) 187, 268
MaeI CTAG 1 cut(s) 56
MaeII ACGT 2 cut(s) 128, 359
MalI GATC 1 cut(s) 116
MboI GATC 1 cut(s) 114
MboII GAAGA 1 cut(s) 159
MluCI AATT 1 cut(s) 137
MnlI CCTC 3 cut(s) 235, 250, 260
MseI TTAA 2 cut(s) 65, 345
MslI CAYNNNNRTG 2 cut(s) 183, 394
MspI CCGG 1 cut(s) 80
MwoI GCNNNNNNNGC 2 cut(s) 180, 332
NdeII GATC 1 cut(s) 114
NlaIII CATG 4 cut(s) 22, 201, 243, 323
PkrI GCNGC 3 cut(s) 217, 325, 387
PmaCI CACGTG 1 cut(s) 360
PmlI CACGTG 1 cut(s) 360
Ppu21I YACGTR 2 cut(s) 129, 360
PspCI CACGTG 1 cut(s) 360
RseI CAYNNNNRTG 2 cut(s) 183, 394
SaqAI TTAA 2 cut(s) 65, 345
SatI GCNGC 3 cut(s) 216, 324, 386
Sau3AI GATC 1 cut(s) 114
SetI ASST 5 cut(s) 131, 227, 271, 328, 362
SfaNI GCATC 2 cut(s) 187, 268
SmiMI CAYNNNNRTG 2 cut(s) 183, 394
Sse9I AATT 1 cut(s) 137
SsiI CCGC 1 cut(s) 42
SspI AATATT 1 cut(s) 63
SspMI CTAG 1 cut(s) 56
TaaI ACNGT 1 cut(s) 158
TaiI ACGT 2 cut(s) 131, 362
TasI AATT 1 cut(s) 137
Tru1I TTAA 2 cut(s) 65, 345
Tru9I TTAA 2 cut(s) 65, 345
TscAI CASTG 3 cut(s) 238, 337, 344
TseI GCWGC 3 cut(s) 215, 323, 385
TspDTI ATGAA 4 cut(s) 7, 228, 308, 423
TspGWI ACGGA 1 cut(s) 341
TspRI CASTG 3 cut(s) 238, 337, 344
XbaI TCTAGA 1 cut(s) 55
XspI CTAG 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.