Rw2G004400

Transcriptional repressor, ovate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0016147
Physical Location & Seq
Reverse (-)
4204668 .. 4205817
1150 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G004400.1

Sequence Viewer

Length: 879 bp
ATGGAAAACCGCTTCAAGCTAAAGCTCTCCCGCATGTTCCACAAGGCCTCACTCGGCACCTGCCGCTCACGCAGCATCTCTGACGTGATCCAAAAAACTGATCACTCTCCTCCCAAGCCAGCTCCTCCTCCTCCTCCTAAGCCTTTCCCGTCCATTTGCAGACCTAAATGCTCCGACGATCCGAAACAGCAACCCATCAACCGCAGCTGCGTCATTTCCAGATCCAACACAGACCTCATGCTCCCCAGACGCAAGATTTTTGAACGTCGCTCCCCATTTGTGAAGCCTGAAATCCCTCCCAATTCTCTGTTCTCGCATTCAAATCCATTCTACGAAGTTCATGAAAGCCATAGCTTTCGACAGAGAAAGAAGAAAGCGACCAAGAAGAAGAAGAAGAAGACGAGGAGTAAGAAAACCGATCTTTTCGCAATCAGCTCTTCTTCTTTGGATAGTAATCACTTTTACAATGGGTGGTGGTCTAGCAGTGAAGATGAAGAGAAAGAAGACGAGACTGATACTCTGTTTTCCTCGAACACTCACACTTCCGATTCGTCCGGGTCTCGCCGGCCACTGCGTTCGAGTTCTCAGCGGAAAGGTCAGAGCTTTGGCCGGAGAAGAGGGGGTTCTGAGGTGGGTCTGATGCCATTGCAAGGAAAAGTGAAGGACAGCTTTGCTGTGGTGAAGAAATCTAGTGATCCTTACAACGATTTCAGAACATCAATGGTGGAAATGATCGTGGAGAAGCAGATATTTTCAGGCAAAGATCTTGACCATCTCTTGCAGTGTTTTCTGAAGCTGAACTCGCCTCATCATCACAGAGTTATTATTGAGGTTTTCACTGAGATTTGGGAGGCTCTGTTCTCCAACTTGGGGACTTGA

Protein Analysis

292

Amino Acids

33.52

Weight (kDa)

9.94

Isoelectric Point (pI)

70.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ovate PF04844 230 - 287 9.1e-26 Transcriptional repressor, ovate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016147)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19650
fragaria_vesca FvH4_1g04770
malus_domestica MD15G1184600.v1.1
prunus_persica Prupe.7G231400_v2.0.a1
pyrus_communis pycom02g03890
rosa_chinensis RchiOBHm_Chr2g0090371
rosa_laevigata RLG00000016103
rosa_multiflora Rmu_sc0004514.1_g000008
rosa_roxburghii Rroxscaffold_2G00151220
rosa_rugosa Rorug02G0006800
rosa_samantha Rh2AG052900 Rh2BG051700 Rh2CG053800 Rh2DG052900
rosa_wichuraiana Rw2G004400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 68
Acc36I ACCTGC 1 cut(s) 68
AccB1I GGYRCC 1 cut(s) 56
AccBSI CCGCTC 1 cut(s) 66
AciI CCGC 5 cut(s) 10, 31, 64, 202, 589
AclWI GGATC 4 cut(s) 82, 173, 216, 689
AcoI YGGCCR 2 cut(s) 566, 607
AcuI CTGAAG 1 cut(s) 812
AfiI CCNNNNNNNGG 2 cut(s) 650, 870
AgsI TTSAA 3 cut(s) 16, 263, 321
AjiI CACGTC 1 cut(s) 85
AjuI GAANNNNNNNTTGG 2 cut(s) 293, 325
AloI GAACNNNNNNTCC 2 cut(s) 842, 874
AluBI AGCT 9 cut(s) 19, 25, 122, 207, 354, 435, 603, 669, 796
AluI AGCT 9 cut(s) 19, 25, 122, 207, 354, 435, 603, 669, 796
Alw26I GTCTC 2 cut(s) 503, 564
AlwI GGATC 4 cut(s) 82, 173, 216, 689
AoxI GGCC 3 cut(s) 45, 566, 607
ApeKI GCWGC 3 cut(s) 72, 204, 207
AsuC2I CCSGG 1 cut(s) 556
AsuHPI GGTGA 1 cut(s) 691
BanI GGYRCC 1 cut(s) 56
BbsI GAAGAC 2 cut(s) 404, 510
BbvI GCAGC 3 cut(s) 84, 194, 216
BccI CCATC 2 cut(s) 203, 780
BclI TGATCA 1 cut(s) 100
BcnI CCSGG 1 cut(s) 556
BcoDI GTCTC 2 cut(s) 503, 564
BfaI CTAG 2 cut(s) 480, 690
BfuAI ACCTGC 1 cut(s) 68
BglII AGATCT 1 cut(s) 763
BisI GCNGC 4 cut(s) 64, 73, 205, 208
BlsI GCNGC 4 cut(s) 65, 74, 206, 209
Bme1390I CCNGG 1 cut(s) 556
BmgBI CACGTC 1 cut(s) 85
BmiI GGNNCC 1 cut(s) 58
BmrFI CCNGG 1 cut(s) 556
BmsI GCATC 2 cut(s) 84, 630
BpiI GAAGAC 2 cut(s) 404, 510
Bpu10I CCTNAGC 1 cut(s) 138
BpuMI CCSGG 1 cut(s) 556
BsaBI GATNNNNATC 1 cut(s) 453
BsaI GGTCTC 1 cut(s) 564
BsaXI ACNNNNNCTCC 2 cut(s) 842, 872
Bsc4I CCNNNNNNNGG 2 cut(s) 650, 870
Bse118I RCCGGY 1 cut(s) 564
Bse3DI GCAATG 1 cut(s) 644
Bse8I GATNNNNATC 1 cut(s) 453
BseJI GATNNNNATC 1 cut(s) 453
BseLI CCNNNNNNNGG 2 cut(s) 650, 870
BseMI GCAATG 1 cut(s) 644
BseMII CTCAG 3 cut(s) 599, 618, 831
BseRI GAGGAG 6 cut(s) 99, 114, 117, 120, 123, 418
BseXI GCAGC 3 cut(s) 84, 194, 216
BshFI GGCC 3 cut(s) 47, 568, 609
BshNI GGYRCC 1 cut(s) 56
BsiSI CCGG 3 cut(s) 555, 565, 610
BslI CCNNNNNNNGG 2 cut(s) 650, 870
BsmAI GTCTC 2 cut(s) 503, 564
BsmI GAATGC 1 cut(s) 316
BsnI GGCC 3 cut(s) 47, 568, 609
Bso31I GGTCTC 1 cut(s) 564
Bsp143I GATC 8 cut(s) 87, 100, 178, 221, 418, 694, 732, 763
BspACI CCGC 5 cut(s) 10, 31, 64, 202, 589
BspANI GGCC 3 cut(s) 47, 568, 609
BspCNI CTCAG 3 cut(s) 598, 619, 832
BspHI TCATGA 1 cut(s) 340
BspLI GGNNCC 1 cut(s) 58
BspMI ACCTGC 1 cut(s) 68
BspPI GGATC 4 cut(s) 82, 173, 216, 689
BspQI GCTCTTC 1 cut(s) 442
BspT107I GGYRCC 1 cut(s) 56
BspTNI GGTCTC 1 cut(s) 564
BsrBI CCGCTC 1 cut(s) 66
BsrDI GCAATG 1 cut(s) 644
BsrFI RCCGGY 1 cut(s) 564
BssAI RCCGGY 1 cut(s) 564
BssMI GATC 8 cut(s) 87, 100, 178, 221, 418, 694, 732, 763
Bst6I CTCTTC 3 cut(s) 442, 489, 610
BstC8I GCNNGC 2 cut(s) 120, 566
BstDEI CTNAG 4 cut(s) 138, 585, 627, 840
BstKTI GATC 8 cut(s) 90, 103, 181, 224, 421, 697, 735, 766
BstMAI GTCTC 2 cut(s) 503, 564
BstMBI GATC 8 cut(s) 87, 100, 178, 221, 418, 694, 732, 763
BstMWI GCNNNNNNNGC 4 cut(s) 63, 69, 72, 802
BstNSI RCATGY 1 cut(s) 37
BstSCI CCNGG 1 cut(s) 554
BstV1I GCAGC 3 cut(s) 84, 194, 216
BstV2I GAAGAC 2 cut(s) 404, 510
BstX2I RGATCY 2 cut(s) 221, 763
BstYI RGATCY 2 cut(s) 221, 763
BsuRI GGCC 3 cut(s) 47, 568, 609
BtrI CACGTC 1 cut(s) 85
BtsI GCAGTG 3 cut(s) 490, 569, 788
BtsIMutI CAGTG 4 cut(s) 490, 569, 788, 837
BveI ACCTGC 1 cut(s) 68
Cac8I GCNNGC 2 cut(s) 120, 566
CciI TCATGA 1 cut(s) 340
Cfr10I RCCGGY 1 cut(s) 564
CseI GACGC 2 cut(s) 199, 258
CviAII CATG 3 cut(s) 34, 238, 341
DdeI CTNAG 4 cut(s) 138, 585, 627, 840
DpnI GATC 8 cut(s) 89, 102, 180, 223, 420, 696, 734, 765
DpnII GATC 8 cut(s) 87, 100, 178, 221, 418, 694, 732, 763
EaeI YGGCCR 2 cut(s) 566, 607
Eam1104I CTCTTC 3 cut(s) 442, 489, 610
EarI CTCTTC 3 cut(s) 442, 489, 610
Eco147I AGGCCT 1 cut(s) 47
Eco31I GGTCTC 1 cut(s) 564
Eco57I CTGAAG 1 cut(s) 812
FaeI CATG 3 cut(s) 37, 241, 344
FaiI YATR 4 cut(s) 35, 239, 342, 351
FalI AAGNNNNNCTT 2 cut(s) 653, 685
FatI CATG 3 cut(s) 33, 237, 340
FauI CCCGC 1 cut(s) 38
FbaI TGATCA 1 cut(s) 100
Fnu4HI GCNGC 4 cut(s) 64, 73, 205, 208
Fsp4HI GCNGC 4 cut(s) 64, 73, 205, 208
FspBI CTAG 2 cut(s) 480, 690
GluI GCNGC 4 cut(s) 64, 73, 205, 208
HaeIII GGCC 3 cut(s) 47, 568, 609
HapII CCGG 3 cut(s) 555, 565, 610
HgaI GACGC 2 cut(s) 199, 258
Hin1II CATG 3 cut(s) 37, 241, 344
HinfI GANTC 1 cut(s) 548
HpaII CCGG 3 cut(s) 555, 565, 610
HphI GGTGA 1 cut(s) 691
Hpy188I TCNGA 9 cut(s) 82, 175, 183, 547, 600, 628, 639, 713, 792
Hpy188III TCNNGA 3 cut(s) 219, 341, 767
Hpy99I CGWCG 2 cut(s) 179, 270
HpyAV CCTTC 1 cut(s) 655
HpyCH4IV ACGT 2 cut(s) 84, 265
HpyCH4V TGCA 3 cut(s) 159, 649, 781
HpyF10VI GCNNNNNNNGC 4 cut(s) 63, 69, 72, 802
HpyF3I CTNAG 4 cut(s) 138, 585, 627, 840
HpySE526I ACGT 2 cut(s) 84, 265
Hsp92II CATG 3 cut(s) 37, 241, 344
KroI GCCGGC 1 cut(s) 564
KroNI GCCGGC 1 cut(s) 566
Ksp22I TGATCA 1 cut(s) 100
Kzo9I GATC 8 cut(s) 87, 100, 178, 221, 418, 694, 732, 763
LguI GCTCTTC 1 cut(s) 442
LmnI GCTCC 4 cut(s) 127, 176, 246, 275
LpnPI CCDG 9 cut(s) 73, 132, 232, 259, 300, 568, 578, 623, 741
Lsp1109I GCAGC 3 cut(s) 84, 194, 216
LweI GCATC 2 cut(s) 84, 630
MaeI CTAG 2 cut(s) 480, 690
MaeII ACGT 2 cut(s) 84, 265
MalI GATC 8 cut(s) 89, 102, 180, 223, 420, 696, 734, 765
MbiI CCGCTC 1 cut(s) 66
MboI GATC 8 cut(s) 87, 100, 178, 221, 418, 694, 732, 763
MflI RGATCY 2 cut(s) 221, 763
MluCI AATT 1 cut(s) 301
MmeI TCCRAC 2 cut(s) 198, 249
MroNI GCCGGC 1 cut(s) 564
MspA1I CMGCKG 2 cut(s) 207, 589
MspI CCGG 3 cut(s) 555, 565, 610
MspR9I CCNGG 1 cut(s) 556
Mva1269I GAATGC 1 cut(s) 316
MwoI GCNNNNNNNGC 4 cut(s) 63, 69, 72, 802
NaeI GCCGGC 1 cut(s) 566
NciI CCSGG 1 cut(s) 556
NdeII GATC 8 cut(s) 87, 100, 178, 221, 418, 694, 732, 763
NgoMIV GCCGGC 1 cut(s) 564
NlaIII CATG 3 cut(s) 37, 241, 344
NlaIV GGNNCC 1 cut(s) 58
NmeAIII GCCGAG 1 cut(s) 33
NspI RCATGY 1 cut(s) 37
PagI TCATGA 1 cut(s) 340
PaqCI CACCTGC 1 cut(s) 68
PceI AGGCCT 1 cut(s) 47
PciSI GCTCTTC 1 cut(s) 442
PctI GAATGC 1 cut(s) 316
PdiI GCCGGC 1 cut(s) 566
PfeI GAWTC 1 cut(s) 548
PkrI GCNGC 4 cut(s) 65, 74, 206, 209
PspN4I GGNNCC 1 cut(s) 58
PsuI RGATCY 2 cut(s) 221, 763
PvuII CAGCTG 1 cut(s) 207
SapI GCTCTTC 1 cut(s) 442
SatI GCNGC 4 cut(s) 64, 73, 205, 208
Sau3AI GATC 8 cut(s) 87, 100, 178, 221, 418, 694, 732, 763
ScrFI CCNGG 1 cut(s) 556
SfaNI GCATC 2 cut(s) 84, 630
Sse9I AATT 1 cut(s) 301
SseBI AGGCCT 1 cut(s) 47
SsiI CCGC 5 cut(s) 10, 31, 64, 202, 589
SspMI CTAG 2 cut(s) 480, 690
StuI AGGCCT 1 cut(s) 47
StyD4I CCNGG 1 cut(s) 554
TaiI ACGT 2 cut(s) 87, 268
TaqI TCGA 3 cut(s) 358, 530, 578
TasI AATT 1 cut(s) 301
TauI GCSGC 1 cut(s) 66
TfiI GAWTC 1 cut(s) 548
TscAI CASTG 4 cut(s) 490, 576, 788, 844
TseI GCWGC 3 cut(s) 72, 204, 207
TspDTI ATGAA 3 cut(s) 329, 357, 507
TspRI CASTG 4 cut(s) 490, 576, 788, 844
XceI RCATGY 1 cut(s) 37
XspI CTAG 2 cut(s) 480, 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.