Rw2G029310

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0010584
Physical Location & Seq
Forward (+)
47383567 .. 47385162
1596 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G029310.1

Sequence Viewer

Length: 972 bp
ATGCTATCACTCTTCAGAAACCACAAATCCAAAGCAAAAGAGGGCTTCTTCCAGAAGAATGGAGCTACCATATTGGAACAGCTCATTCATGATTTCAATGGTAATTGTAATCCGATTCAATTGTACTCCGCAGAAGAGCTCAAGAAGGCAACAAATGACTACCACAGTGACGGAATGAGATATGACGGTTGGAACTTCCGAGTGTACAAAGGAGTTCATGGAGGTCGTGAAATTTTGGTGAAAAATTTTGGGGAAAGATCAAACTACAGGAGTAGTGAATTTTTAATTGCAACTGAACTCGCAGTTTCTTCAAACATGAGCAATCACAAGAACGTTTTGAAGCTCTTGGGATGTTGCCTTGAGACGGAGTTGGCCACTCTGGTTTTTGAGTTCCCAGCAAAAGGAAATCTCCCTCATCACATTTACGGAGATAATGCACAATCACTGCCATGGAAGACCAAGTTAAAGATCGCAATTGAAGTAGCAGATGCAGTTTCATATCTTCACCACGGCTCTTCCAAGGTGTTCATTCATAGGAATATAAGAACTGAAAATATCTTCCTGGACCAGGACTGCGTCGCCAAGCTGTCGGATTTCCAAGTGTCACTACCAATTCCTTTGGACCAAGACCATGTGTTTTCCGAATGTCTCAGTTTCTTAGGGCACTTATCTCCAGAAGTACTGATGTATAATCGTTATACAAAGAAAAGTGACGTCTTTAACTTGGGGATGATAATGTGCGAAATTTTGACAGGAAAGAAAATTTATGAGCTACTAAGACTTCGGTCTTCTGGGGATTCGAAGGAACTGGAAAACATTATGGAGGGAAATTTACTGCATGAGAGCAACATGAGGCAAGTAACAGAATGCAGCAAGCTCGCAGTAAGGTGTCTCGACTCAGATCCTAATGCAAGACCCACGGCGCAAGAAGTCTCACAGACACTCAGATCGATCAAAAGGTTCCAGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

323

Amino Acids

36.81

Weight (kDa)

7.64

Isoelectric Point (pI)

46.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 67 - 313 9.3e-34 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 75 - 315 2e-35 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 717
AciI CCGC 1 cut(s) 129
AclI AACGTT 1 cut(s) 333
AclWI GGATC 1 cut(s) 896
AcoI YGGCCR 1 cut(s) 372
AcsI RAATTY 6 cut(s) 231, 244, 278, 744, 762, 829
AcyI GRCGYC 1 cut(s) 714
AfaI GTAC 3 cut(s) 125, 206, 681
AfiI CCNNNNNNNGG 3 cut(s) 364, 401, 568
AgsI TTSAA 5 cut(s) 97, 119, 312, 340, 479
AjnI CCWGG 2 cut(s) 561, 567
AluBI AGCT 8 cut(s) 65, 82, 139, 343, 586, 772, 877, 967
AluI AGCT 8 cut(s) 65, 82, 139, 343, 586, 772, 877, 967
Alw21I GWGCWC 1 cut(s) 141
Alw26I GTCTC 4 cut(s) 356, 653, 896, 937
AlwI GGATC 1 cut(s) 896
AoxI GGCC 1 cut(s) 372
ApeKI GCWGC 1 cut(s) 870
ApoI RAATTY 6 cut(s) 231, 244, 278, 744, 762, 829
AspLEI GCGC 1 cut(s) 925
AspS9I GGNCC 2 cut(s) 565, 622
AsuHPI GGTGA 2 cut(s) 250, 497
AsuII TTCGAA 1 cut(s) 800
AvaII GGWCC 2 cut(s) 565, 622
BaeGI GKGCMC 1 cut(s) 666
BalI TGGCCA 1 cut(s) 374
BanII GRGCYC 1 cut(s) 141
BbsI GAAGAC 2 cut(s) 461, 780
Bbv12I GWGCWC 1 cut(s) 141
BbvI GCAGC 1 cut(s) 882
BceAI ACGGC 2 cut(s) 526, 936
BcgI CGANNNNNNTGC 2 cut(s) 859, 893
BciT130I CCWGG 2 cut(s) 563, 569
BcoDI GTCTC 4 cut(s) 356, 653, 896, 937
BfmI CTRYAG 1 cut(s) 265
BisI GCNGC 1 cut(s) 871
BlsI GCNGC 1 cut(s) 872
BmcAI AGTACT 1 cut(s) 681
Bme1390I CCNGG 2 cut(s) 563, 569
Bme18I GGWCC 2 cut(s) 565, 622
BmgT120I GGNCC 2 cut(s) 565, 622
BmiI GGNNCC 1 cut(s) 962
BmrFI CCNGG 2 cut(s) 563, 569
BmsI GCATC 1 cut(s) 478
BoxI GACNNNNGTC 1 cut(s) 784
BpiI GAAGAC 2 cut(s) 461, 780
BpmI CTGGAG 1 cut(s) 657
Bpu14I TTCGAA 1 cut(s) 800
BpuEI CTTGAG 2 cut(s) 125, 380
Bsa29I ATCGAT 1 cut(s) 950
BsaHI GRCGYC 1 cut(s) 714
BsaJI CCNNGG 4 cut(s) 449, 508, 519, 918
Bsc4I CCNNNNNNNGG 3 cut(s) 364, 401, 568
Bse1I ACTGG 1 cut(s) 813
BseBI CCWGG 2 cut(s) 563, 569
BseCI ATCGAT 1 cut(s) 950
BseDI CCNNGG 4 cut(s) 449, 508, 519, 918
BseGI GGATG 2 cut(s) 356, 735
BseLI CCNNNNNNNGG 3 cut(s) 364, 401, 568
BseMII CTCAG 3 cut(s) 664, 912, 958
BseNI ACTGG 1 cut(s) 813
BseSI GKGCMC 1 cut(s) 666
BseXI GCAGC 1 cut(s) 882
BseYI CCCAGC 1 cut(s) 394
BshFI GGCC 1 cut(s) 374
BshVI ATCGAT 1 cut(s) 950
BsiHKAI GWGCWC 1 cut(s) 141
BslI CCNNNNNNNGG 3 cut(s) 364, 401, 568
BsmAI GTCTC 4 cut(s) 356, 653, 896, 937
BsmBI CGTCTC 1 cut(s) 356
BsmI GAATGC 1 cut(s) 872
BsnI GGCC 1 cut(s) 374
Bsp119I TTCGAA 1 cut(s) 800
Bsp1286I GDGCHC 2 cut(s) 141, 666
Bsp1407I TGTACA 1 cut(s) 204
Bsp143I GATC 5 cut(s) 257, 468, 901, 947, 951
Bsp19I CCATGG 1 cut(s) 449
BspACI CCGC 1 cut(s) 129
BspANI GGCC 1 cut(s) 374
BspCNI CTCAG 3 cut(s) 663, 911, 957
BspDI ATCGAT 1 cut(s) 950
BspHI TCATGA 1 cut(s) 88
BspLI GGNNCC 1 cut(s) 962
BspPI GGATC 1 cut(s) 896
BspQI GCTCTTC 2 cut(s) 129, 520
BspT104I TTCGAA 1 cut(s) 800
BsrGI TGTACA 1 cut(s) 204
BsrI ACTGG 1 cut(s) 813
BssECI CCNNGG 4 cut(s) 449, 508, 519, 918
BssMI GATC 5 cut(s) 257, 468, 901, 947, 951
BssNI GRCGYC 1 cut(s) 714
BssT1I CCWWGG 2 cut(s) 449, 519
Bst2UI CCWGG 2 cut(s) 563, 569
Bst4CI ACNGT 2 cut(s) 167, 188
Bst6I CTCTTC 3 cut(s) 17, 129, 520
BstACI GRCGYC 1 cut(s) 714
BstAUI TGTACA 1 cut(s) 204
BstBI TTCGAA 1 cut(s) 800
BstC8I GCNNGC 2 cut(s) 875, 879
BstDEI CTNAG 5 cut(s) 650, 658, 776, 898, 944
BstDSI CCRYGG 3 cut(s) 449, 508, 918
BstENI CCTNNNNNAGG 1 cut(s) 566
BstF5I GGATG 2 cut(s) 356, 735
BstHHI GCGC 1 cut(s) 925
BstKTI GATC 5 cut(s) 260, 471, 904, 950, 954
BstMAI GTCTC 4 cut(s) 356, 653, 896, 937
BstMBI GATC 5 cut(s) 257, 468, 901, 947, 951
BstNI CCWGG 2 cut(s) 563, 569
BstPAI GACNNNNGTC 1 cut(s) 784
BstSCI CCNGG 2 cut(s) 561, 567
BstSFI CTRYAG 1 cut(s) 265
BstSLI GKGCMC 1 cut(s) 666
BstV1I GCAGC 1 cut(s) 882
BstV2I GAAGAC 2 cut(s) 461, 780
BstX2I RGATCY 1 cut(s) 901
BstXI CCANNNNNNTGG 1 cut(s) 59
BstYI RGATCY 1 cut(s) 901
Bsu15I ATCGAT 1 cut(s) 950
BsuRI GGCC 1 cut(s) 374
BsuTUI ATCGAT 1 cut(s) 950
BtgI CCRYGG 3 cut(s) 449, 508, 918
BtsCI GGATG 2 cut(s) 356, 735
BtsI GCAGTG 1 cut(s) 443
BtsIMutI CAGTG 2 cut(s) 172, 443
Cac8I GCNNGC 2 cut(s) 875, 879
CciI TCATGA 1 cut(s) 88
CfoI GCGC 1 cut(s) 925
Cfr13I GGNCC 2 cut(s) 565, 622
ClaI ATCGAT 1 cut(s) 950
CseI GACGC 1 cut(s) 565
Csp6I GTAC 3 cut(s) 124, 205, 680
CviAII CATG 7 cut(s) 89, 218, 316, 450, 632, 839, 850
CviQI GTAC 3 cut(s) 124, 205, 680
DdeI CTNAG 5 cut(s) 650, 658, 776, 898, 944
DpnI GATC 5 cut(s) 259, 470, 903, 949, 953
DpnII GATC 5 cut(s) 257, 468, 901, 947, 951
EaeI YGGCCR 1 cut(s) 372
Eam1104I CTCTTC 3 cut(s) 17, 129, 520
EarI CTCTTC 3 cut(s) 17, 129, 520
Ecl136II GAGCTC 1 cut(s) 139
Eco130I CCWWGG 2 cut(s) 449, 519
Eco24I GRGCYC 1 cut(s) 141
Eco47I GGWCC 2 cut(s) 565, 622
Eco53kI GAGCTC 1 cut(s) 139
EcoICRI GAGCTC 1 cut(s) 139
EcoNI CCTNNNNNAGG 1 cut(s) 566
EcoRII CCWGG 2 cut(s) 561, 567
EcoT14I CCWWGG 2 cut(s) 449, 519
EcoT38I GRGCYC 1 cut(s) 141
ErhI CCWWGG 2 cut(s) 449, 519
Esp3I CGTCTC 1 cut(s) 356
FaeI CATG 7 cut(s) 92, 221, 319, 453, 635, 842, 853
FatI CATG 7 cut(s) 88, 217, 315, 449, 631, 838, 849
Fnu4HI GCNGC 1 cut(s) 871
FokI GGATG 2 cut(s) 363, 742
FriOI GRGCYC 1 cut(s) 141
Fsp4HI GCNGC 1 cut(s) 871
GlaI GCGC 1 cut(s) 924
GluI GCNGC 1 cut(s) 871
GsaI CCCAGC 1 cut(s) 398
GsuI CTGGAG 1 cut(s) 657
HaeIII GGCC 1 cut(s) 374
HgaI GACGC 1 cut(s) 565
HhaI GCGC 1 cut(s) 925
Hin1I GRCGYC 1 cut(s) 714
Hin1II CATG 7 cut(s) 92, 221, 319, 453, 635, 842, 853
Hin6I GCGC 1 cut(s) 923
HinP1I GCGC 1 cut(s) 923
HinfI GANTC 3 cut(s) 115, 797, 896
HphI GGTGA 2 cut(s) 250, 497
Hpy166II GTNNAC 1 cut(s) 205
Hpy188I TCNGA 7 cut(s) 17, 114, 200, 592, 643, 901, 947
Hpy188III TCNNGA 6 cut(s) 52, 89, 142, 227, 674, 893
Hpy8I GTNNAC 1 cut(s) 205
Hpy99I CGWCG 1 cut(s) 581
HpyAV CCTTC 2 cut(s) 139, 796
HpyCH4III ACNGT 2 cut(s) 167, 188
HpyCH4IV ACGT 2 cut(s) 333, 714
HpyCH4V TGCA 6 cut(s) 290, 437, 491, 838, 870, 911
HpyF3I CTNAG 5 cut(s) 650, 658, 776, 898, 944
HpySE526I ACGT 2 cut(s) 333, 714
Hsp92I GRCGYC 1 cut(s) 714
Hsp92II CATG 7 cut(s) 92, 221, 319, 453, 635, 842, 853
HspAI GCGC 1 cut(s) 923
Kzo9I GATC 5 cut(s) 257, 468, 901, 947, 951
LguI GCTCTTC 2 cut(s) 129, 520
LmnI GCTCC 1 cut(s) 62
Lsp1109I GCAGC 1 cut(s) 882
LweI GCATC 1 cut(s) 478
MaeII ACGT 2 cut(s) 333, 714
MaeIII GTNAC 4 cut(s) 167, 603, 710, 859
MalI GATC 5 cut(s) 259, 470, 903, 949, 953
MboI GATC 5 cut(s) 257, 468, 901, 947, 951
MfeI CAATTG 2 cut(s) 119, 474
MflI RGATCY 1 cut(s) 901
MhlI GDGCHC 2 cut(s) 141, 666
MlsI TGGCCA 1 cut(s) 374
MluNI TGGCCA 1 cut(s) 374
MlyI GAGTC 1 cut(s) 890
MmeI TCCRAC 2 cut(s) 170, 570
MnlI CCTC 5 cut(s) 34, 215, 423, 817, 846
Mox20I TGGCCA 1 cut(s) 374
MscI TGGCCA 1 cut(s) 374
MseI TTAA 4 cut(s) 284, 464, 720, 970
MslI CAYNNNNRTG 1 cut(s) 448
Msp20I TGGCCA 1 cut(s) 374
MspR9I CCNGG 2 cut(s) 563, 569
MunI CAATTG 2 cut(s) 119, 474
Mva1269I GAATGC 1 cut(s) 872
MvaI CCWGG 2 cut(s) 563, 569
NcoI CCATGG 1 cut(s) 449
NdeII GATC 5 cut(s) 257, 468, 901, 947, 951
NlaIII CATG 7 cut(s) 92, 221, 319, 453, 635, 842, 853
NlaIV GGNNCC 1 cut(s) 962
NmuCI GTSAC 3 cut(s) 167, 603, 710
NspV TTCGAA 1 cut(s) 800
PagI TCATGA 1 cut(s) 88
PciSI GCTCTTC 2 cut(s) 129, 520
PctI GAATGC 1 cut(s) 872
PfeI GAWTC 2 cut(s) 115, 797
PflFI GACNNNGTC 1 cut(s) 575
PfoI TCCNGGA 1 cut(s) 561
PkrI GCNGC 1 cut(s) 872
PleI GAGTC 1 cut(s) 890
PpsI GAGTC 1 cut(s) 890
PshAI GACNNNNGTC 1 cut(s) 784
Psp124BI GAGCTC 1 cut(s) 141
Psp1406I AACGTT 1 cut(s) 333
Psp6I CCWGG 2 cut(s) 561, 567
PspFI CCCAGC 1 cut(s) 394
PspGI CCWGG 2 cut(s) 561, 567
PspN4I GGNNCC 1 cut(s) 962
PspPI GGNCC 2 cut(s) 565, 622
PsuI RGATCY 1 cut(s) 901
PsyI GACNNNGTC 1 cut(s) 575
RsaI GTAC 3 cut(s) 125, 206, 681
RsaNI GTAC 3 cut(s) 124, 205, 680
RseI CAYNNNNRTG 1 cut(s) 448
SacI GAGCTC 1 cut(s) 141
SapI GCTCTTC 2 cut(s) 129, 520
SaqAI TTAA 4 cut(s) 284, 464, 720, 970
SatI GCNGC 1 cut(s) 871
Sau3AI GATC 5 cut(s) 257, 468, 901, 947, 951
Sau96I GGNCC 2 cut(s) 565, 622
ScaI AGTACT 1 cut(s) 681
SchI GAGTC 1 cut(s) 890
ScrFI CCNGG 2 cut(s) 563, 569
SduI GDGCHC 2 cut(s) 141, 666
SfaNI GCATC 1 cut(s) 478
SfcI CTRYAG 1 cut(s) 265
SfuI TTCGAA 1 cut(s) 800
SinI GGWCC 2 cut(s) 565, 622
SmiMI CAYNNNNRTG 1 cut(s) 448
SmlI CTYRAG 2 cut(s) 140, 359
SmoI CTYRAG 2 cut(s) 140, 359
SsiI CCGC 1 cut(s) 129
SstI GAGCTC 1 cut(s) 141
StyD4I CCNGG 2 cut(s) 561, 567
StyI CCWWGG 2 cut(s) 449, 519
TaaI ACNGT 2 cut(s) 167, 188
TaiI ACGT 2 cut(s) 336, 717
TaqI TCGA 3 cut(s) 800, 894, 950
TaqII GACCGA 1 cut(s) 774
TatI WGTACW 3 cut(s) 123, 204, 679
TfiI GAWTC 2 cut(s) 115, 797
Tru1I TTAA 4 cut(s) 284, 464, 720, 970
Tru9I TTAA 4 cut(s) 284, 464, 720, 970
TscAI CASTG 2 cut(s) 172, 450
TseFI GTSAC 3 cut(s) 167, 603, 710
TseI GCWGC 1 cut(s) 870
Tsp45I GTSAC 3 cut(s) 167, 603, 710
TspDTI ATGAA 5 cut(s) 77, 206, 486, 517, 521
TspGWI ACGGA 3 cut(s) 186, 380, 441
TspRI CASTG 2 cut(s) 172, 450
Tth111I GACNNNGTC 1 cut(s) 575
VpaK11BI GGWCC 2 cut(s) 565, 622
XagI CCTNNNNNAGG 1 cut(s) 566
XapI RAATTY 6 cut(s) 231, 244, 278, 744, 762, 829
ZraI GACGTC 1 cut(s) 715
ZrmI AGTACT 1 cut(s) 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.