Rw3G017010

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0009624
Physical Location & Seq
Reverse (-)
18339740 .. 18341182
1443 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G017010.1

Sequence Viewer

Length: 1443 bp
ATGAGGAGGTTCCGAAAACCAAAGCTGATACCTTTACTGGAGCAAAAGCCTCCATCATCACTACCACAGCTATCTCCGATCCCAAACCCATCTCTCCCAAAACCCCCACAACACCCATCACCACCACCTTCCCCAATCCTCACTCTCTCCCACACCTCAAACCACACCCACCTCCTCACCTTCATCAAAACCCACATCAAAAACCCACCTCTCTCCCCCAAAACCCTTCTCCATTTCCTCAAATCCAAGCTCCACCACCACCCCACCTTCACCCATTTCGACTTCCACGTCTTCAACTGGGCCTCCTCCGTCGACTCCTTCCGCCACGACCACTCCACTTTCGAATGGATGGTCTGCACTCTCGCAACCACCGACCGCTTCGCCGAGCTGGGCCATCTCATCGGTTTCATGGTTTCGAACCCATGCCCTTGTTCCGATGGTATTTTCTCTTGCCCAAGAACAGAGCCCATTTTCAAATTTGCGATTAGTGCTTATTGTAGAGTAGGGAGATTGGGCGATGCTGTTAGTGCTTTTGATTCCATGAGGAAATTGATTGATGGGAAGCCTAGTGTTGTGGTTTATAACATTGTGATTAATGGGTTTGTGAAATGTGGGGCACATGATAAGGCTTTGGGGTTTTATGAGAAGATGGGGAGGGATAGAGTAAGGGGTGATGTGTTTACTTTCAATATTTTGATTAGTAGTTATTGTCGAAATTCGCAATTCGGGTTGGCTTTGGAGTTGTTTAAGGAGATGAGGGAGAAGGGGTGTAGTCCCAATGTGGTTAGTTTCAATACTTTGATTAAGGGGTTCTTTAGGGAGAGGAAGTTTGAGGATGGGATTGAGATGGCTTATGAGATGATTGAGTTTGGGTGCAAGTTTTCGAGTGTGACTTGTGAGATTTTGGTTGATGGACTTTGTAGAGAAGGTCGGGTTGAGGAGGCGTGTGAGCTGTTGATTGATTTTTCAAGGAAAGGAGTGTTGCCTAGATTGTATGATTACTATGGTCTTGTTGAAGTGCTTTGTGGGGAAAGAAATTCGGGGAGAGCGTTAGAGGTTGTGGATGAGCTGTGGAGGACGGGAAATGTTCCGAGCTTGATTGCTTGTACCACTTTGGTTGAGGGTTTGAGGAGGTCGGCGAGGATTGATGAAGCTTTAGAAGTGATGGAAAGGATGCTTGAAGAGGGTATGGTTCCAGATATTGTGACTTTTAATTGCCTCCTTCAAGATCTTTGCAATTTGGGGAGAACTGAGGAAGCTGATAACCTTAGGTTGTTGTCTTCTAGTAAAGGCTTGGAGCTAGACGGCATGGCCTATACCATTTTGGTGTCTGGGTATTCAAGAGAAGGGAAAAAGAAGGAGGTGAAAGTGGTGGTGGATGAGATGCTGGATATGGGATTTATACCTGATCTTGCTACATATAATAGGTTGGTTGATGGATGA

Protein Analysis

480

Amino Acids

54.19

Weight (kDa)

6.74

Isoelectric Point (pI)

39.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 189 - 238 2.6e-14 PPR repeat family
PPR_3 PF13812 215 - 269 3.3e-12 Pentatricopeptide repeat domain
PPR_2 PF13041 225 - 271 1.3e-17 PPR repeat family
PPR_1 PF12854 225 - 253 1.3e-08 PPR repeat
PPR PF01535 227 - 257 9.6e-10 PPR repeat
PPR_1 PF12854 255 - 287 2.6e-07 PPR repeat
PPR_2 PF13041 259 - 308 6.7e-12 PPR repeat family
PPR_1 PF12854 291 - 319 4.2e-07 PPR repeat
PPR_3 PF13812 353 - 409 1.2e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 364 - 413 1.7e-09 PPR repeat family
PPR_3 PF13812 429 - 477 8.6e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 438 - 480 7.4e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 582
AasI GACNNNNNNGTC 1 cut(s) 287
AccI GTMKAC 1 cut(s) 312
AciI CCGC 2 cut(s) 322, 376
AclWI GGATC 1 cut(s) 73
AcsI RAATTY 3 cut(s) 476, 715, 1036
AfaI GTAC 1 cut(s) 1108
AgsI TTSAA 9 cut(s) 295, 475, 688, 793, 969, 1016, 1181, 1226, 1341
AjiI CACGTC 1 cut(s) 289
AlwI GGATC 1 cut(s) 73
AoxI GGCC 3 cut(s) 300, 391, 1311
ApoI RAATTY 3 cut(s) 476, 715, 1036
ArsI GACNNNNNNTTYG 2 cut(s) 336, 368
AseI ATTAAT 1 cut(s) 594
AspS9I GGNCC 2 cut(s) 300, 391
AsuHPI GGTGA 5 cut(s) 111, 169, 262, 683, 1375
AsuII TTCGAA 2 cut(s) 342, 416
AxyI CCTNAGG 1 cut(s) 1268
BaeGI GKGCMC 1 cut(s) 619
BanII GRGCYC 1 cut(s) 468
BbsI GAAGAC 2 cut(s) 283, 1272
BceAI ACGGC 1 cut(s) 1321
BfaI CTAG 4 cut(s) 567, 987, 1284, 1301
BglII AGATCT 1 cut(s) 1228
BmgBI CACGTC 1 cut(s) 289
BmgT120I GGNCC 2 cut(s) 300, 391
BmiI GGNNCC 2 cut(s) 11, 1194
BmrI ACTGGG 1 cut(s) 307
BmsI GCATC 3 cut(s) 508, 1164, 1374
BmuI ACTGGG 1 cut(s) 307
BpiI GAAGAC 2 cut(s) 283, 1272
BpmI CTGGAG 1 cut(s) 59
Bpu14I TTCGAA 2 cut(s) 342, 416
BsaXI ACNNNNNCTCC 8 cut(s) 287, 317, 317, 347, 752, 782, 812, 842
Bse1I ACTGG 2 cut(s) 42, 302
Bse21I CCTNAGG 1 cut(s) 1268
BseGI GGATG 5 cut(s) 354, 841, 1069, 1179, 1384
BseMII CTCAG 1 cut(s) 1242
BseNI ACTGG 2 cut(s) 42, 302
BseRI GAGGAG 5 cut(s) 19, 164, 295, 953, 1144
BseSI GKGCMC 1 cut(s) 619
BseYI CCCAGC 1 cut(s) 388
BsgI GTGCAG 1 cut(s) 340
Bsh1285I CGRYCG 1 cut(s) 376
BshFI GGCC 3 cut(s) 302, 393, 1313
BsiEI CGRYCG 1 cut(s) 376
BslFI GGGAC 1 cut(s) 759
BsmFI GGGAC 1 cut(s) 759
BsnI GGCC 3 cut(s) 302, 393, 1313
Bsp119I TTCGAA 2 cut(s) 342, 416
Bsp1286I GDGCHC 2 cut(s) 468, 619
Bsp143I GATC 3 cut(s) 78, 1228, 1408
BspACI CCGC 2 cut(s) 322, 376
BspANI GGCC 3 cut(s) 302, 393, 1313
BspCNI CTCAG 1 cut(s) 1243
BspLI GGNNCC 2 cut(s) 11, 1194
BspPI GGATC 1 cut(s) 73
BspT104I TTCGAA 2 cut(s) 342, 416
BsrI ACTGG 2 cut(s) 42, 302
BssMI GATC 3 cut(s) 78, 1228, 1408
Bst6I CTCTTC 1 cut(s) 1176
BstBI TTCGAA 2 cut(s) 342, 416
BstDEI CTNAG 2 cut(s) 1251, 1268
BstF5I GGATG 5 cut(s) 354, 841, 1069, 1179, 1384
BstKTI GATC 3 cut(s) 81, 1231, 1411
BstMBI GATC 3 cut(s) 78, 1228, 1408
BstMCI CGRYCG 1 cut(s) 376
BstMWI GCNNNNNNNGC 2 cut(s) 488, 527
BstSLI GKGCMC 1 cut(s) 619
BstV2I GAAGAC 2 cut(s) 283, 1272
BstX2I RGATCY 1 cut(s) 1228
BstYI RGATCY 1 cut(s) 1228
Bsu36I CCTNAGG 1 cut(s) 1268
BsuRI GGCC 3 cut(s) 302, 393, 1313
BtgZI GCGATG 1 cut(s) 531
BtrI CACGTC 1 cut(s) 289
BtsCI GGATG 5 cut(s) 354, 841, 1069, 1179, 1384
Cfr13I GGNCC 2 cut(s) 300, 391
Csp6I GTAC 1 cut(s) 1107
CspCI CAANNNNNGTGG 2 cut(s) 1099, 1134
CviAII CATG 5 cut(s) 409, 423, 541, 620, 1309
CviQI GTAC 1 cut(s) 1107
DdeI CTNAG 2 cut(s) 1251, 1268
DpnI GATC 3 cut(s) 80, 1230, 1410
DpnII GATC 3 cut(s) 78, 1228, 1408
DrdI GACNNNNNNGTC 1 cut(s) 287
DseDI GACNNNNNNGTC 1 cut(s) 287
Eam1104I CTCTTC 1 cut(s) 1176
EarI CTCTTC 1 cut(s) 1176
EciI GGCGGA 1 cut(s) 311
Eco24I GRGCYC 1 cut(s) 468
Eco81I CCTNAGG 1 cut(s) 1268
EcoT38I GRGCYC 1 cut(s) 468
FaeI CATG 5 cut(s) 412, 426, 544, 623, 1312
FalI AAGNNNNNCTT 2 cut(s) 797, 829
FaqI GGGAC 1 cut(s) 759
FatI CATG 5 cut(s) 408, 422, 540, 619, 1308
FblI GTMKAC 1 cut(s) 312
FokI GGATG 5 cut(s) 361, 848, 1076, 1186, 1391
FriOI GRGCYC 1 cut(s) 468
FspBI CTAG 4 cut(s) 567, 987, 1284, 1301
GsaI CCCAGC 1 cut(s) 392
GsuI CTGGAG 1 cut(s) 59
HaeIII GGCC 3 cut(s) 302, 393, 1313
Hin1II CATG 5 cut(s) 412, 426, 544, 623, 1312
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HindIII AAGCTT 1 cut(s) 1152
HinfI GANTC 2 cut(s) 314, 536
HphI GGTGA 5 cut(s) 111, 169, 262, 683, 1375
Hpy166II GTNNAC 2 cut(s) 313, 681
Hpy188I TCNGA 4 cut(s) 14, 78, 436, 1092
Hpy188III TCNNGA 3 cut(s) 1196, 1226, 1341
Hpy8I GTNNAC 2 cut(s) 313, 681
Hpy99I CGWCG 1 cut(s) 314
HpyCH4IV ACGT 1 cut(s) 288
HpyCH4V TGCA 3 cut(s) 357, 876, 1236
HpyF10VI GCNNNNNNNGC 2 cut(s) 488, 527
HpyF3I CTNAG 2 cut(s) 1251, 1268
HpySE526I ACGT 1 cut(s) 288
Hsp92II CATG 5 cut(s) 412, 426, 544, 623, 1312
Kzo9I GATC 3 cut(s) 78, 1228, 1408
LmnI GCTCC 3 cut(s) 40, 255, 1297
LpnPI CCDG 7 cut(s) 23, 283, 374, 1209, 1317, 1373, 1419
LweI GCATC 3 cut(s) 508, 1164, 1374
MaeI CTAG 4 cut(s) 567, 987, 1284, 1301
MaeII ACGT 1 cut(s) 288
MaeIII GTNAC 2 cut(s) 889, 1204
MalI GATC 3 cut(s) 80, 1230, 1410
MboI GATC 3 cut(s) 78, 1228, 1408
MboII GAAGA 4 cut(s) 283, 658, 1193, 1272
MflI RGATCY 1 cut(s) 1228
MhlI GDGCHC 2 cut(s) 468, 619
MluCI AATT 7 cut(s) 476, 548, 715, 722, 1036, 1213, 1237
MlyI GAGTC 1 cut(s) 308
MseI TTAA 4 cut(s) 594, 747, 804, 1212
MslI CAYNNNNRTG 1 cut(s) 1325
MwoI GCNNNNNNNGC 2 cut(s) 488, 527
NdeII GATC 3 cut(s) 78, 1228, 1408
NlaIII CATG 5 cut(s) 412, 426, 544, 623, 1312
NlaIV GGNNCC 2 cut(s) 11, 1194
NmeAIII GCCGAG 1 cut(s) 409
NmuCI GTSAC 2 cut(s) 889, 1204
NspV TTCGAA 2 cut(s) 342, 416
PcsI WCGNNNNNNNCGW 3 cut(s) 285, 369, 1046
PfeI GAWTC 1 cut(s) 536
PleI GAGTC 1 cut(s) 308
PpsI GAGTC 1 cut(s) 308
PshBI ATTAAT 1 cut(s) 594
PsiI TTATAA 1 cut(s) 582
PspFI CCCAGC 1 cut(s) 388
PspN4I GGNNCC 2 cut(s) 11, 1194
PspPI GGNCC 2 cut(s) 300, 391
PsuI RGATCY 1 cut(s) 1228
RsaI GTAC 1 cut(s) 1108
RsaNI GTAC 1 cut(s) 1107
RseI CAYNNNNRTG 1 cut(s) 1325
SalI GTCGAC 1 cut(s) 311
SaqAI TTAA 4 cut(s) 594, 747, 804, 1212
Sau3AI GATC 3 cut(s) 78, 1228, 1408
Sau96I GGNCC 2 cut(s) 300, 391
SchI GAGTC 1 cut(s) 308
SduI GDGCHC 2 cut(s) 468, 619
SfaNI GCATC 3 cut(s) 508, 1164, 1374
SfuI TTCGAA 2 cut(s) 342, 416
SmiMI CAYNNNNRTG 1 cut(s) 1325
Sse9I AATT 7 cut(s) 476, 548, 715, 722, 1036, 1213, 1237
SsiI CCGC 2 cut(s) 322, 376
SspI AATATT 1 cut(s) 691
SspMI CTAG 4 cut(s) 567, 987, 1284, 1301
TaiI ACGT 1 cut(s) 291
TaqI TCGA 6 cut(s) 279, 312, 342, 416, 712, 884
TasI AATT 7 cut(s) 476, 548, 715, 722, 1036, 1213, 1237
TfiI GAWTC 1 cut(s) 536
Tru1I TTAA 4 cut(s) 594, 747, 804, 1212
Tru9I TTAA 4 cut(s) 594, 747, 804, 1212
TseFI GTSAC 2 cut(s) 889, 1204
Tsp45I GTSAC 2 cut(s) 889, 1204
TspDTI ATGAA 3 cut(s) 172, 397, 1164
TspGWI ACGGA 1 cut(s) 298
VspI ATTAAT 1 cut(s) 594
XapI RAATTY 3 cut(s) 476, 715, 1036
XmiI GTMKAC 1 cut(s) 312
XspI CTAG 4 cut(s) 567, 987, 1284, 1301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.