Rw4G032600

Protein TWIN LOV

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0005796
Physical Location & Seq
Forward (+)
59690941 .. 59694582
3642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G032600.1

Sequence Viewer

Length: 1194 bp
ATGGAGTCGGAGTTGGGTTTACTAGAGCAATCGATCAATAGTCGTTACTCGCTTTGCGTCCAAGAAGCTCTGAACGAATTGACGGACAGTTTTACGGTGACTGATCCTTATATTTCCGGCCAACCCATCGTTTTTGCGAGCAAAGGGTTCCTGAAAATGATGGGGTACTCGAAAACCGAGGTGATTGGGAGGAATGGGAGGATATTTCAAGGGCCAGGTACTTGTAGAAGGTCGGTTATGGAAATAAGGGAGGCCATTAGAGAAGAGAGAGGTATTCAGATTAATTTGGTGAATTATAGGAGGGATGGGACACCCTTCTGGATGCTCTTTCATATGTTCCCTGTGTTTAGCAAAGAGGATGGGAGGGTGATTCATTTTGTGGGAGTTCAAGTTCCCATTTTGCGAAAACCGAGGAGGAGGAATGGGGTTGGTTTGGTGTATGAGGAGGAGAGTAGGGTGAATGAGAGTGTTTATGGGTCTTGTAGAAGAGAAGTGTGCTCTGATTCTTTGGTGGATTTGGGCAGTGCTTTGTCTCTTGAGTGTGCACTGGATGATGCTGATAATAGAGGACTGGAGATTGAAGAGACGTGTGAGGCAAGTGAATTAGAGAAGCAAAGAGCTGCGTCTTCCATGAATAACATCTTGTCTGTGCTAACACATTATAGCCAGTTAACAGGCAGACTGGTGTGTGGAAAGAGATGCAACTTAACTGGGGTGGGCCTTCTCAGTTCATCCTTAAATATTTCTCTTGGTAGAATCAAACAAAGCTTTGTATTAACAGATCCGCACTTACCTGACATGCCTATAGTTTACGCAAGTGAGGCCTTCTTCAAATTGACAGGTTATGCCAGACACGAAGTCATGGGACGCAATTGTAGATTTCTAAGTGGCGAGGATACGGATTCCTCAGCGATATATCGGATAAAGGAAAGCGTTCAGAATGAGAAAGCATGCACAGTACGTATCTTGAATTACAGGAAGGATAAGAGTTCATTTTGGAATCTTCTTCATGTATCACCAATTCGTAATGCTTCTGGCAAGATTGCGTACTTTGTGGGTGTTCAGATGGAAGAAGCCTGTAAGAACCAGGATGGGCATGGGCTAAGCCCTGAGATGAGGCAGCTTAGCGCCGTTGGAGCGGTCAGGATTGCAGTGAGGAGTTTATCAATGGGTGCCGGCACATCCGAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS_9

Protein Analysis

397

Amino Acids

44.29

Weight (kDa)

7.53

Isoelectric Point (pI)

51.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_9 PF13426 42 - 130 2.2e-16 PAS domain
PAS_3 PF08447 43 - 129 2.3e-06 PAS fold
PAS_9 PF13426 257 - 355 7.1e-18 PAS domain
PAS PF00989 269 - 355 1.2e-08 PAS fold
PAS_3 PF08447 269 - 354 3.6e-06 PAS fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1172
AccBSI CCGCTC 1 cut(s) 1139
AciI CCGC 2 cut(s) 785, 1139
AclWI GGATC 2 cut(s) 98, 776
AcoI YGGCCR 1 cut(s) 118
AfaI GTAC 4 cut(s) 167, 220, 960, 1049
AflIII ACRYGT 1 cut(s) 587
AgsI TTSAA 5 cut(s) 209, 389, 581, 832, 970
AhdI GACNNNNNGTC 1 cut(s) 857
AjiI CACGTC 1 cut(s) 588
AjnI CCWGG 2 cut(s) 214, 1086
AloI GAACNNNNNNTCC 2 cut(s) 375, 407
AluBI AGCT 4 cut(s) 68, 620, 768, 1123
AluI AGCT 4 cut(s) 68, 620, 768, 1123
Alw21I GWGCWC 2 cut(s) 500, 547
Alw26I GTCTC 2 cut(s) 537, 578
Alw44I GTGCAC 1 cut(s) 543
AlwI GGATC 2 cut(s) 98, 776
AoxI GGCC 5 cut(s) 118, 212, 252, 718, 822
ApaLI GTGCAC 1 cut(s) 543
ApeKI GCWGC 2 cut(s) 620, 1120
AseI ATTAAT 1 cut(s) 282
Asp700I GAANNNNTTC 1 cut(s) 933
AspLEI GCGC 1 cut(s) 1130
AspS9I GGNCC 2 cut(s) 212, 718
AsuHPI GGTGA 6 cut(s) 109, 193, 301, 379, 469, 1008
BaeGI GKGCMC 1 cut(s) 547
BanI GGYRCC 1 cut(s) 1172
BbsI GAAGAC 1 cut(s) 618
Bbv12I GWGCWC 2 cut(s) 500, 547
BbvCI CCTCAGC 1 cut(s) 907
BbvI GCAGC 2 cut(s) 607, 1132
BccI CCATC 6 cut(s) 134, 154, 299, 353, 1060, 1085
BceAI ACGGC 1 cut(s) 1115
BciT130I CCWGG 2 cut(s) 216, 1088
BciVI GTATCC 1 cut(s) 889
BcoDI GTCTC 2 cut(s) 537, 578
BfaI CTAG 1 cut(s) 23
BfmI CTRYAG 1 cut(s) 804
BfoI RGCGCY 1 cut(s) 1131
BfuI GTATCC 1 cut(s) 889
BisI GCNGC 2 cut(s) 621, 1121
BlpI GCTNAGC 2 cut(s) 1103, 1124
BlsI GCNGC 2 cut(s) 622, 1122
Bme1390I CCNGG 2 cut(s) 216, 1088
BmeRI GACNNNNNGTC 1 cut(s) 857
BmgBI CACGTC 1 cut(s) 588
BmgT120I GGNCC 2 cut(s) 212, 718
BmiI GGNNCC 2 cut(s) 149, 1174
BmrFI CCNGG 2 cut(s) 216, 1088
BmrI ACTGGG 1 cut(s) 720
BmsI GCATC 3 cut(s) 312, 544, 689
BmuI ACTGGG 1 cut(s) 720
BpiI GAAGAC 1 cut(s) 618
BpmI CTGGAG 1 cut(s) 593
Bpu10I CCTNAGC 1 cut(s) 907
Bpu1102I GCTNAGC 2 cut(s) 1103, 1124
BpuEI CTTGAG 1 cut(s) 557
Bsa29I ATCGAT 1 cut(s) 32
BsaAI YACGTR 1 cut(s) 962
BsaJI CCNNGG 2 cut(s) 177, 410
BsaXI ACNNNNNCTCC 3 cut(s) 26, 375, 405
Bse118I RCCGGY 1 cut(s) 1175
Bse1I ACTGG 5 cut(s) 552, 576, 667, 687, 715
BseBI CCWGG 2 cut(s) 216, 1088
BseCI ATCGAT 1 cut(s) 32
BseDI CCNNGG 2 cut(s) 177, 410
BseGI GGATG 7 cut(s) 310, 327, 364, 556, 731, 1096, 1181
BseMII CTCAG 3 cut(s) 739, 921, 1101
BseNI ACTGG 5 cut(s) 552, 576, 667, 687, 715
BseRI GAGGAG 5 cut(s) 427, 430, 458, 461, 1171
BseSI GKGCMC 1 cut(s) 547
BseXI GCAGC 2 cut(s) 607, 1132
BshFI GGCC 5 cut(s) 120, 214, 254, 720, 824
BshNI GGYRCC 1 cut(s) 1172
BshVI ATCGAT 1 cut(s) 32
BsiHKAI GWGCWC 2 cut(s) 500, 547
BsiSI CCGG 2 cut(s) 117, 1176
BslFI GGGAC 2 cut(s) 322, 879
BsmAI GTCTC 2 cut(s) 537, 578
BsmBI CGTCTC 1 cut(s) 578
BsmFI GGGAC 2 cut(s) 322, 879
BsnI GGCC 5 cut(s) 120, 214, 254, 720, 824
Bsp1286I GDGCHC 2 cut(s) 500, 547
Bsp143I GATC 3 cut(s) 33, 103, 781
Bsp1720I GCTNAGC 2 cut(s) 1103, 1124
BspACI CCGC 2 cut(s) 785, 1139
BspANI GGCC 5 cut(s) 120, 214, 254, 720, 824
BspCNI CTCAG 3 cut(s) 738, 920, 1102
BspDI ATCGAT 1 cut(s) 32
BspLI GGNNCC 2 cut(s) 149, 1174
BspPI GGATC 2 cut(s) 98, 776
BspT107I GGYRCC 1 cut(s) 1172
BsrBI CCGCTC 1 cut(s) 1139
BsrFI RCCGGY 1 cut(s) 1175
BsrI ACTGG 5 cut(s) 552, 576, 667, 687, 715
BssAI RCCGGY 1 cut(s) 1175
BssECI CCNNGG 2 cut(s) 177, 410
BssMI GATC 3 cut(s) 33, 103, 781
Bst2UI CCWGG 2 cut(s) 216, 1088
Bst4CI ACNGT 3 cut(s) 89, 97, 958
Bst6I CTCTTC 3 cut(s) 258, 481, 576
BstBAI YACGTR 1 cut(s) 962
BstC8I GCNNGC 3 cut(s) 139, 952, 1177
BstDEI CTNAG 6 cut(s) 725, 884, 907, 1103, 1110, 1124
BstF5I GGATG 7 cut(s) 310, 327, 364, 556, 731, 1096, 1181
BstH2I RGCGCY 1 cut(s) 1131
BstHHI GCGC 1 cut(s) 1130
BstKTI GATC 3 cut(s) 36, 106, 784
BstMAI GTCTC 2 cut(s) 537, 578
BstMBI GATC 3 cut(s) 33, 103, 781
BstMWI GCNNNNNNNGC 2 cut(s) 821, 1136
BstNI CCWGG 2 cut(s) 216, 1088
BstNSI RCATGY 2 cut(s) 802, 954
BstSCI CCNGG 2 cut(s) 214, 1086
BstSFI CTRYAG 1 cut(s) 804
BstSLI GKGCMC 1 cut(s) 547
BstSNI TACGTA 1 cut(s) 962
BstV1I GCAGC 2 cut(s) 607, 1132
BstV2I GAAGAC 1 cut(s) 618
BstX2I RGATCY 1 cut(s) 781
BstYI RGATCY 1 cut(s) 781
Bsu15I ATCGAT 1 cut(s) 32
BsuI GTATCC 1 cut(s) 889
BsuRI GGCC 5 cut(s) 120, 214, 254, 720, 824
BsuTUI ATCGAT 1 cut(s) 32
BtrI CACGTC 1 cut(s) 588
BtsCI GGATG 7 cut(s) 310, 327, 364, 556, 731, 1096, 1181
BtsI GCAGTG 2 cut(s) 529, 1158
BtsIMutI CAGTG 3 cut(s) 529, 545, 1158
Cac8I GCNNGC 3 cut(s) 139, 952, 1177
CfoI GCGC 1 cut(s) 1130
Cfr10I RCCGGY 1 cut(s) 1175
Cfr13I GGNCC 2 cut(s) 212, 718
ClaI ATCGAT 1 cut(s) 32
CseI GACGC 3 cut(s) 46, 612, 876
Csp6I GTAC 4 cut(s) 166, 219, 959, 1048
CviAII CATG 6 cut(s) 631, 799, 862, 951, 1010, 1097
CviQI GTAC 4 cut(s) 166, 219, 959, 1048
DdeI CTNAG 6 cut(s) 725, 884, 907, 1103, 1110, 1124
DpnI GATC 3 cut(s) 35, 105, 783
DpnII GATC 3 cut(s) 33, 103, 781
DriI GACNNNNNGTC 1 cut(s) 857
EaeI YGGCCR 1 cut(s) 118
Eam1104I CTCTTC 3 cut(s) 258, 481, 576
Eam1105I GACNNNNNGTC 1 cut(s) 857
EarI CTCTTC 3 cut(s) 258, 481, 576
Eco105I TACGTA 1 cut(s) 962
Eco147I AGGCCT 1 cut(s) 824
EcoRII CCWGG 2 cut(s) 214, 1086
Esp3I CGTCTC 1 cut(s) 578
FaeI CATG 6 cut(s) 634, 802, 865, 954, 1013, 1100
FaqI GGGAC 2 cut(s) 322, 879
FatI CATG 6 cut(s) 630, 798, 861, 950, 1009, 1096
FauNDI CATATG 1 cut(s) 333
Fnu4HI GCNGC 2 cut(s) 621, 1121
FokI GGATG 7 cut(s) 317, 334, 371, 563, 718, 1103, 1168
Fsp4HI GCNGC 2 cut(s) 621, 1121
FspBI CTAG 1 cut(s) 23
GlaI GCGC 1 cut(s) 1129
GluI GCNGC 2 cut(s) 621, 1121
GsuI CTGGAG 1 cut(s) 593
HaeII RGCGCY 1 cut(s) 1131
HaeIII GGCC 5 cut(s) 120, 214, 254, 720, 824
HapII CCGG 2 cut(s) 117, 1176
HgaI GACGC 3 cut(s) 46, 612, 876
HhaI GCGC 1 cut(s) 1130
Hin1II CATG 6 cut(s) 634, 802, 865, 954, 1013, 1100
Hin6I GCGC 1 cut(s) 1128
HinP1I GCGC 1 cut(s) 1128
HincII GTYRAC 1 cut(s) 672
HindII GTYRAC 1 cut(s) 672
HindIII AAGCTT 1 cut(s) 766
HinfI GANTC 7 cut(s) 5, 370, 503, 756, 902, 1000, 1187
HpaI GTTAAC 1 cut(s) 672
HpaII CCGG 2 cut(s) 117, 1176
HphI GGTGA 6 cut(s) 109, 193, 301, 379, 469, 1008
Hpy166II GTNNAC 4 cut(s) 20, 545, 672, 811
Hpy188I TCNGA 8 cut(s) 10, 72, 279, 502, 921, 939, 1065, 1186
Hpy188III TCNNGA 6 cut(s) 151, 319, 536, 967, 1144, 1191
Hpy8I GTNNAC 4 cut(s) 20, 545, 672, 811
HpyAV CCTTC 5 cut(s) 222, 325, 731, 835, 973
HpyCH4III ACNGT 3 cut(s) 89, 97, 958
HpyCH4IV ACGT 2 cut(s) 587, 961
HpyCH4V TGCA 4 cut(s) 545, 702, 954, 1151
HpyF10VI GCNNNNNNNGC 2 cut(s) 821, 1136
HpyF3I CTNAG 6 cut(s) 725, 884, 907, 1103, 1110, 1124
HpySE526I ACGT 2 cut(s) 587, 961
Hsp92II CATG 6 cut(s) 634, 802, 865, 954, 1013, 1100
HspAI GCGC 1 cut(s) 1128
KroI GCCGGC 1 cut(s) 1175
KroNI GCCGGC 1 cut(s) 1177
KspAI GTTAAC 1 cut(s) 672
Kzo9I GATC 3 cut(s) 33, 103, 781
LmnI GCTCC 1 cut(s) 1136
Lsp1109I GCAGC 2 cut(s) 607, 1132
LweI GCATC 3 cut(s) 312, 544, 689
MaeI CTAG 1 cut(s) 23
MaeII ACGT 2 cut(s) 587, 961
MaeIII GTNAC 2 cut(s) 44, 97
MalI GATC 3 cut(s) 35, 105, 783
MbiI CCGCTC 1 cut(s) 1139
MboI GATC 3 cut(s) 33, 103, 781
MboII GAAGA 8 cut(s) 275, 498, 593, 618, 820, 995, 998, 1082
MfeI CAATTG 1 cut(s) 871
MflI RGATCY 1 cut(s) 781
MhlI GDGCHC 2 cut(s) 500, 547
MluCI AATT 8 cut(s) 77, 283, 292, 602, 833, 871, 970, 1020
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 1 cut(s) 1114
MroNI GCCGGC 1 cut(s) 1175
MroXI GAANNNNTTC 1 cut(s) 933
MseI TTAA 5 cut(s) 282, 671, 707, 737, 776
MspI CCGG 2 cut(s) 117, 1176
MspR9I CCNGG 2 cut(s) 216, 1088
MunI CAATTG 1 cut(s) 871
MvaI CCWGG 2 cut(s) 216, 1088
MwoI GCNNNNNNNGC 2 cut(s) 821, 1136
NaeI GCCGGC 1 cut(s) 1177
NdeI CATATG 1 cut(s) 333
NdeII GATC 3 cut(s) 33, 103, 781
NgoMIV GCCGGC 1 cut(s) 1175
NlaIII CATG 6 cut(s) 634, 802, 865, 954, 1013, 1100
NlaIV GGNNCC 2 cut(s) 149, 1174
NmuCI GTSAC 1 cut(s) 97
NspI RCATGY 2 cut(s) 802, 954
PaeI GCATGC 1 cut(s) 954
PceI AGGCCT 1 cut(s) 824
PdiI GCCGGC 1 cut(s) 1177
PdmI GAANNNNTTC 1 cut(s) 933
PfeI GAWTC 5 cut(s) 370, 503, 756, 902, 1000
PkrI GCNGC 2 cut(s) 622, 1122
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
Ppu21I YACGTR 1 cut(s) 962
PshBI ATTAAT 1 cut(s) 282
Psp6I CCWGG 2 cut(s) 214, 1086
PspGI CCWGG 2 cut(s) 214, 1086
PspN4I GGNNCC 2 cut(s) 149, 1174
PspPI GGNCC 2 cut(s) 212, 718
PsuI RGATCY 1 cut(s) 781
RsaI GTAC 4 cut(s) 167, 220, 960, 1049
RsaNI GTAC 4 cut(s) 166, 219, 959, 1048
SaqAI TTAA 5 cut(s) 282, 671, 707, 737, 776
SatI GCNGC 2 cut(s) 621, 1121
Sau3AI GATC 3 cut(s) 33, 103, 781
Sau96I GGNCC 2 cut(s) 212, 718
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 2 cut(s) 216, 1088
SduI GDGCHC 2 cut(s) 500, 547
SfaNI GCATC 3 cut(s) 312, 544, 689
SfcI CTRYAG 1 cut(s) 804
SmlI CTYRAG 1 cut(s) 536
SmoI CTYRAG 1 cut(s) 536
SnaBI TACGTA 1 cut(s) 962
SphI GCATGC 1 cut(s) 954
Sse9I AATT 8 cut(s) 77, 283, 292, 602, 833, 871, 970, 1020
SseBI AGGCCT 1 cut(s) 824
SsiI CCGC 2 cut(s) 785, 1139
SspI AATATT 1 cut(s) 742
SspMI CTAG 1 cut(s) 23
StuI AGGCCT 1 cut(s) 824
StyD4I CCNGG 2 cut(s) 214, 1086
TaaI ACNGT 3 cut(s) 89, 97, 958
TaiI ACGT 2 cut(s) 590, 964
TaqI TCGA 2 cut(s) 32, 170
TasI AATT 8 cut(s) 77, 283, 292, 602, 833, 871, 970, 1020
TfiI GAWTC 5 cut(s) 370, 503, 756, 902, 1000
Tru1I TTAA 5 cut(s) 282, 671, 707, 737, 776
Tru9I TTAA 5 cut(s) 282, 671, 707, 737, 776
TscAI CASTG 3 cut(s) 529, 552, 1158
TseFI GTSAC 1 cut(s) 97
TseI GCWGC 2 cut(s) 620, 1120
Tsp45I GTSAC 1 cut(s) 97
TspDTI ATGAA 6 cut(s) 320, 362, 647, 720, 981, 998
TspGWI ACGGA 2 cut(s) 98, 914
TspRI CASTG 3 cut(s) 529, 552, 1158
VneI GTGCAC 1 cut(s) 543
VspI ATTAAT 1 cut(s) 282
XceI RCATGY 2 cut(s) 802, 954
XcmI CCANNNNNNNNNTGG 1 cut(s) 1094
XmnI GAANNNNTTC 1 cut(s) 933
XspI CTAG 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.