Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0016280

Physical Location & Seq

Genomic Coordinates Forward (+)

3649753 .. 3651975

Gene Structure 2223 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G003710.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1134 bp

ATGTCCGCACTTCTCCATCACTCACAAACCACATCGCTAATTATACTCCTTTCATCATTTTTCTCCCTCCTCAAATTTGTTTCTTGCGATTCCAATTCCCAGAATCACCCGCTTCACTACATCCACCATTACTGTTCCCAAGAATACAATCTCACTGACAACCCGGGGTTCCACGACAACGTCGACATCCTCCTCTCCGATCTCACCTCCAAATCTTCTGGATCCAGCTTCTACAACTCCACGGCCGGAGACGACCACGAAAAAGTCTATGCTCTTTACCTCTGTCGAGGCGACATTAGCTCTCAAGTTTGCCATAACTGCATCGACCATGCAGCTCAAACCCTCCGGGAAAACTGCACCGACTTCCAAGACACCACCGTGTATTATGAGGAGTGCATGTTGCGATATTCAAACCGTTCCATTTTCGCCACGGAGCAGGAGCTTCCATTTCGATTTTGGTGCAGCGTGAAAAAGGTTTCCGACAACGCTACCGATGATTTTAATCAGAGTCTGACCAAGTTGATGGGCTGGCTTGTGGATAAGGCAGCGTTTGGTAATACAACTCCCATTCATTTCTCGACAGGGGAAGCAAATACGAAAACCGGTGATAATGGCACGTCGTGGTTGTATGGCTTGATGCAGTGCACGCCTGATATAACTGGGAGTGATTGCGAAAAATGTTTGAAGGCGGCTATCGGCAGTTACCAGGAGTTCTGCGCCGAAAGAATGTGGGCGATGATTTTAGCCCCTAGTTGTCAGTTACGGTATAATACAAGCCCTTTTATGAAGACAGAAAAACCCGCCATGCCAAAGAGTTCAACTGATGGAAGAGGGAAGAACTACAAATCATATGTTGCTCTTGCAACAGTGGTGCTTCTGTCCACCATATTTGTTGCCGAAACCCATAAAGGCGGTGTTGATGAACGCCTAGTCTATGGTGGCGCCTTGTCTGGGTTGCCTCCTAAATCTGGGCTCGAGGAGAGATTGATGCTTGAGCGACGTGGACAAGAATTCTTTGAGGCGAAGAGTGGCGTTGGGCTGATCGCCAACTCTATTGGCTGCTCTAATCACAGGGCTGCTGGCGTTCTGTCCGGCCGTCGTCGGTGGTGGAATTGTTGCTCCGATCCGCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009507 GO:0009532 GO:0009534 GO:0009535 GO:0009536 GO:0009570 GO:0009579 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010275 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022607 GO:0031224 GO:0031225 GO:0031226 GO:0031976 GO:0031984 GO:0034357 GO:0034622 GO:0036211 GO:0042651 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046658 GO:0050896 GO:0051704 GO:0051707 GO:0055035 GO:0065003 GO:0071704 GO:0071840 GO:0071944 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase Stress-antifung

Protein Analysis

377

Amino Acids

41.99

Weight (kDa)

6.11

Isoelectric Point (pI)

43.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Stress-antifung	PF01657	45 - 139	1.3e-21	Salt stress response/antifungal
Stress-antifung	PF01657	154 - 258	6.1e-12	Salt stress response/antifungal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016280)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g02832
malus_domestica	MD00G1101500.v1.1 MD10G1312400.v1.1
prunus_persica	Prupe.4G028400_v2.0.a1
pyrus_communis	pycom10g26370
rosa_chinensis	RchiOBHm_Chr5g0004281
rosa_laevigata	RLG00000031218
rosa_multiflora	Rmu_co8460447.1_g000001
rosa_roxburghii	Rroxscaffold_1G00071390
rosa_rugosa	Rorug04G0411100
rosa_samantha	Rh5BG037900 Rh5CG041600 Rh5DG037200
rosa_wichuraiana	Rw5G003510 Rw5G003710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	941
AccBSI	CCGCTC	1 cut(s)	1129
AccI	GTMKAC	1 cut(s)	183
AciI	CCGC	6 cut(s)	6, 110, 689, 801, 912, 1127
AclWI	GGATC	3 cut(s)	216, 229, 1118
AcoI	YGGCCR	2 cut(s)	243, 1093
AcsI	RAATTY	2 cut(s)	74, 1010
AcyI	GRCGYC	1 cut(s)	942
AdeI	CACNNNGTG	1 cut(s)	621
AfiI	CCNNNNNNNGG	2 cut(s)	951, 968
AgeI	ACCGGT	1 cut(s)	602
AgsI	TTSAA	3 cut(s)	411, 685, 819
AjiI	CACGTC	2 cut(s)	618, 1001
AjnI	CCWGG	1 cut(s)	705
AleI	CACNNNNGTG	1 cut(s)	377
AluBI	AGCT	4 cut(s)	228, 300, 335, 442
AluI	AGCT	4 cut(s)	228, 300, 335, 442
Alw21I	GWGCWC	1 cut(s)	647
Alw26I	GTCTC	1 cut(s)	243
Alw44I	GTGCAC	1 cut(s)	643
AlwI	GGATC	3 cut(s)	216, 229, 1118
AlwNI	CAGNNNCTG	1 cut(s)	511
Ama87I	CYCGRG	2 cut(s)	163, 974
AoxI	GGCC	2 cut(s)	243, 1093
ApaLI	GTGCAC	1 cut(s)	643
ApeKI	GCWGC	5 cut(s)	332, 462, 545, 1059, 1076
ApoI	RAATTY	2 cut(s)	74, 1010
AsiGI	ACCGGT	1 cut(s)	602
AspLEI	GCGC	2 cut(s)	719, 944
AsuC2I	CCSGG	3 cut(s)	164, 165, 347
AsuHPI	GGTGA	3 cut(s)	98, 196, 617
AvaI	CYCGRG	2 cut(s)	163, 974
BaeGI	GKGCMC	1 cut(s)	647
BamHI	GGATCC	1 cut(s)	221
BanI	GGYRCC	1 cut(s)	941
BanII	GRGCYC	1 cut(s)	975
BbsI	GAAGAC	1 cut(s)	794
Bbv12I	GWGCWC	1 cut(s)	647
BbvI	GCAGC	5 cut(s)	344, 474, 557, 1046, 1063
BccI	CCATC	3 cut(s)	24, 517, 818
BceAI	ACGGC	2 cut(s)	258, 1080
BcgI	CGANNNNNNTGC	2 cut(s)	441, 475
BciT130I	CCWGG	1 cut(s)	707
BcnI	CCSGG	3 cut(s)	164, 165, 347
BcoDI	GTCTC	1 cut(s)	243
BfaI	CTAG	2 cut(s)	750, 929
BfoI	RGCGCY	1 cut(s)	945
BisI	GCNGC	6 cut(s)	333, 463, 546, 690, 1060, 1077
BlsI	GCNGC	6 cut(s)	334, 464, 547, 691, 1061, 1078
Bme1390I	CCNGG	4 cut(s)	164, 165, 347, 707
BmeT110I	CYCGRG	2 cut(s)	163, 974
BmgBI	CACGTC	2 cut(s)	618, 1001
BmiI	GGNNCC	3 cut(s)	170, 223, 943
BmrFI	CCNGG	4 cut(s)	164, 165, 347, 707
BmrI	ACTGGG	1 cut(s)	669
BmsI	GCATC	3 cut(s)	330, 627, 978
BmuI	ACTGGG	1 cut(s)	669
BpiI	GAAGAC	1 cut(s)	794
BpuEI	CTTGAG	2 cut(s)	288, 1013
BpuMI	CCSGG	3 cut(s)	164, 165, 347
BsaBI	GATNNNNATC	1 cut(s)	501
BsaHI	GRCGYC	1 cut(s)	942
BsaJI	CCNNGG	4 cut(s)	163, 164, 240, 429
BsaWI	WCCGGW	1 cut(s)	602
BsaXI	ACNNNNNCTCC	2 cut(s)	383, 413
Bsc4I	CCNNNNNNNGG	2 cut(s)	951, 968
Bse118I	RCCGGY	1 cut(s)	602
Bse1I	ACTGG	1 cut(s)	664
Bse8I	GATNNNNATC	1 cut(s)	501
BseBI	CCWGG	1 cut(s)	707
BseDI	CCNNGG	4 cut(s)	163, 164, 240, 429
BseGI	GGATG	2 cut(s)	120, 186
BseJI	GATNNNNATC	1 cut(s)	501
BseLI	CCNNNNNNNGG	2 cut(s)	951, 968
BseNI	ACTGG	1 cut(s)	664
BseRI	GAGGAG	4 cut(s)	59, 182, 404, 992
BseSI	GKGCMC	1 cut(s)	647
BseX3I	CGGCCG	2 cut(s)	243, 1093
BseXI	GCAGC	5 cut(s)	344, 474, 557, 1046, 1063
BsgI	GTGCAG	2 cut(s)	340, 481
Bsh1285I	CGRYCG	2 cut(s)	246, 1096
BshFI	GGCC	2 cut(s)	245, 1095
BshNI	GGYRCC	1 cut(s)	941
BshTI	ACCGGT	1 cut(s)	602
BsiEI	CGRYCG	2 cut(s)	246, 1096
BsiHKAI	GWGCWC	1 cut(s)	647
BsiHKCI	CYCGRG	2 cut(s)	163, 974
BsiSI	CCGG	5 cut(s)	164, 246, 346, 603, 1092
BslI	CCNNNNNNNGG	2 cut(s)	951, 968
BsmAI	GTCTC	1 cut(s)	243
BsmBI	CGTCTC	1 cut(s)	243
BsnI	GGCC	2 cut(s)	245, 1095
BsoBI	CYCGRG	2 cut(s)	163, 974
Bsp1286I	GDGCHC	2 cut(s)	647, 975
Bsp143I	GATC	4 cut(s)	199, 221, 1041, 1123
BspACI	CCGC	6 cut(s)	6, 110, 689, 801, 912, 1127
BspANI	GGCC	2 cut(s)	245, 1095
BspLI	GGNNCC	3 cut(s)	170, 223, 943
BspPI	GGATC	3 cut(s)	216, 229, 1118
BspT107I	GGYRCC	1 cut(s)	941
BsrBI	CCGCTC	1 cut(s)	1129
BsrFI	RCCGGY	1 cut(s)	602
BsrI	ACTGG	1 cut(s)	664
BssAI	RCCGGY	1 cut(s)	602
BssECI	CCNNGG	4 cut(s)	163, 164, 240, 429
BssMI	GATC	4 cut(s)	199, 221, 1041, 1123
BssNI	GRCGYC	1 cut(s)	942
Bst2UI	CCWGG	1 cut(s)	707
Bst4CI	ACNGT	5 cut(s)	134, 379, 416, 765, 868
Bst6I	CTCTTC	2 cut(s)	823, 1019
BstACI	GRCGYC	1 cut(s)	942
BstC8I	GCNNGC	3 cut(s)	530, 647, 1081
BstDSI	CCRYGG	2 cut(s)	240, 429
BstF5I	GGATG	2 cut(s)	120, 186
BstH2I	RGCGCY	1 cut(s)	945
BstHHI	GCGC	2 cut(s)	719, 944
BstKTI	GATC	4 cut(s)	202, 224, 1044, 1126
BstMAI	GTCTC	1 cut(s)	243
BstMBI	GATC	4 cut(s)	199, 221, 1041, 1123
BstMCI	CGRYCG	2 cut(s)	246, 1096
BstMWI	GCNNNNNNNGC	3 cut(s)	297, 318, 646
BstNI	CCWGG	1 cut(s)	707
BstNSI	RCATGY	1 cut(s)	400
BstSCI	CCNGG	4 cut(s)	162, 163, 345, 705
BstSLI	GKGCMC	1 cut(s)	647
BstV1I	GCAGC	5 cut(s)	344, 474, 557, 1046, 1063
BstV2I	GAAGAC	1 cut(s)	794
BstX2I	RGATCY	1 cut(s)	221
BstXI	CCANNNNNNTGG	1 cut(s)	523
BstYI	RGATCY	1 cut(s)	221
BstZI	CGGCCG	2 cut(s)	243, 1093
BsuRI	GGCC	2 cut(s)	245, 1095
BtgI	CCRYGG	2 cut(s)	240, 429
BtgZI	GCGATG	2 cut(s)	18, 749
BtrI	CACGTC	2 cut(s)	618, 1001
BtsCI	GGATG	2 cut(s)	120, 186
BtsI	GCAGTG	1 cut(s)	647
BtsIMutI	CAGTG	3 cut(s)	153, 647, 873
Cac8I	GCNNGC	3 cut(s)	530, 647, 1081
CaiI	CAGNNNCTG	1 cut(s)	511
CfoI	GCGC	2 cut(s)	719, 944
Cfr10I	RCCGGY	1 cut(s)	602
Cfr9I	CCCGGG	1 cut(s)	163
CspAI	ACCGGT	1 cut(s)	602
CspCI	CAANNNNNGTGG	2 cut(s)	871, 906
CviAII	CATG	3 cut(s)	329, 397, 805
DinI	GGCGCC	1 cut(s)	943
DpnI	GATC	4 cut(s)	201, 223, 1043, 1125
DpnII	GATC	4 cut(s)	199, 221, 1041, 1123
DraIII	CACNNNGTG	1 cut(s)	621
EaeI	YGGCCR	2 cut(s)	243, 1093
EagI	CGGCCG	2 cut(s)	243, 1093
Eam1104I	CTCTTC	2 cut(s)	823, 1019
EarI	CTCTTC	2 cut(s)	823, 1019
EclXI	CGGCCG	2 cut(s)	243, 1093
Eco24I	GRGCYC	1 cut(s)	975
Eco52I	CGGCCG	2 cut(s)	243, 1093
Eco88I	CYCGRG	2 cut(s)	163, 974
EcoRI	GAATTC	1 cut(s)	1010
EcoRII	CCWGG	1 cut(s)	705
EcoT38I	GRGCYC	1 cut(s)	975
EgeI	GGCGCC	1 cut(s)	943
EheI	GGCGCC	1 cut(s)	943
Esp3I	CGTCTC	1 cut(s)	243
FaeI	CATG	3 cut(s)	332, 400, 808
FatI	CATG	3 cut(s)	328, 396, 804
FauI	CCCGC	2 cut(s)	117, 808
FauNDI	CATATG	1 cut(s)	850
FblI	GTMKAC	1 cut(s)	183
Fnu4HI	GCNGC	6 cut(s)	333, 463, 546, 690, 1060, 1077
FokI	GGATG	2 cut(s)	107, 173
FriOI	GRGCYC	1 cut(s)	975
Fsp4HI	GCNGC	6 cut(s)	333, 463, 546, 690, 1060, 1077
FspBI	CTAG	2 cut(s)	750, 929
GlaI	GCGC	2 cut(s)	718, 943
GluI	GCNGC	6 cut(s)	333, 463, 546, 690, 1060, 1077
HaeII	RGCGCY	1 cut(s)	945
HaeIII	GGCC	2 cut(s)	245, 1095
HapII	CCGG	5 cut(s)	164, 246, 346, 603, 1092
HhaI	GCGC	2 cut(s)	719, 944
Hin1I	GRCGYC	1 cut(s)	942
Hin1II	CATG	3 cut(s)	332, 400, 808
Hin6I	GCGC	2 cut(s)	717, 942
HinP1I	GCGC	2 cut(s)	717, 942
HincII	GTYRAC	1 cut(s)	184
HindII	GTYRAC	1 cut(s)	184
HinfI	GANTC	3 cut(s)	89, 103, 508
HpaII	CCGG	5 cut(s)	164, 246, 346, 603, 1092
HphI	GGTGA	3 cut(s)	98, 196, 617
Hpy166II	GTNNAC	4 cut(s)	184, 645, 882, 1004
Hpy188I	TCNGA	6 cut(s)	199, 481, 507, 513, 1123, 1133
Hpy188III	TCNNGA	2 cut(s)	219, 577
Hpy8I	GTNNAC	4 cut(s)	184, 645, 882, 1004
Hpy99I	CGWCG	5 cut(s)	185, 622, 1002, 1101, 1104
HpyAV	CCTTC	1 cut(s)	679
HpyCH4III	ACNGT	5 cut(s)	134, 379, 416, 765, 868
HpyCH4IV	ACGT	3 cut(s)	180, 617, 1000
HpyCH4V	TGCA	8 cut(s)	321, 332, 357, 396, 462, 640, 645, 863
HpyF10VI	GCNNNNNNNGC	3 cut(s)	297, 318, 646
HpySE526I	ACGT	3 cut(s)	180, 617, 1000
Hsp92I	GRCGYC	1 cut(s)	942
Hsp92II	CATG	3 cut(s)	332, 400, 808
HspAI	GCGC	2 cut(s)	717, 942
KasI	GGCGCC	1 cut(s)	941
Kzo9I	GATC	4 cut(s)	199, 221, 1041, 1123
LmnI	GCTCC	3 cut(s)	433, 439, 1124
Lsp1109I	GCAGC	5 cut(s)	344, 474, 557, 1046, 1063
LweI	GCATC	3 cut(s)	330, 627, 978
MaeI	CTAG	2 cut(s)	750, 929
MaeII	ACGT	3 cut(s)	180, 617, 1000
MaeIII	GTNAC	2 cut(s)	701, 759
MalI	GATC	4 cut(s)	201, 223, 1043, 1125
MbiI	CCGCTC	1 cut(s)	1129
MboI	GATC	4 cut(s)	199, 221, 1041, 1123
MboII	GAAGA	5 cut(s)	207, 799, 840, 847, 1036
MflI	RGATCY	1 cut(s)	221
MhlI	GDGCHC	2 cut(s)	647, 975
MluCI	AATT	5 cut(s)	39, 74, 94, 1010, 1111
Mly113I	GGCGCC	1 cut(s)	942
MlyI	GAGTC	1 cut(s)	517
MmeI	TCCRAC	1 cut(s)	504
MseI	TTAA	1 cut(s)	501
MslI	CAYNNNNRTG	1 cut(s)	377
MspI	CCGG	5 cut(s)	164, 246, 346, 603, 1092
MspR9I	CCNGG	4 cut(s)	164, 165, 347, 707
MvaI	CCWGG	1 cut(s)	707
MwoI	GCNNNNNNNGC	3 cut(s)	297, 318, 646
NarI	GGCGCC	1 cut(s)	942
NciI	CCSGG	3 cut(s)	164, 165, 347
NdeI	CATATG	1 cut(s)	850
NdeII	GATC	4 cut(s)	199, 221, 1041, 1123
NlaIII	CATG	3 cut(s)	332, 400, 808
NlaIV	GGNNCC	3 cut(s)	170, 223, 943
NspI	RCATGY	1 cut(s)	400
OliI	CACNNNNGTG	1 cut(s)	377
PaeR7I	CTCGAG	1 cut(s)	974
PcsI	WCGNNNNNNNCGW	1 cut(s)	180
PfeI	GAWTC	2 cut(s)	89, 103
PflFI	GACNNNGTC	1 cut(s)	179
PfoI	TCCNGGA	1 cut(s)	345
PinAI	ACCGGT	1 cut(s)	602
PkrI	GCNGC	6 cut(s)	334, 464, 547, 691, 1061, 1078
PleI	GAGTC	1 cut(s)	516
PluTI	GGCGCC	1 cut(s)	945
PpsI	GAGTC	1 cut(s)	516
Psp6I	CCWGG	1 cut(s)	705
PspGI	CCWGG	1 cut(s)	705
PspN4I	GGNNCC	3 cut(s)	170, 223, 943
PspXI	VCTCGAGB	1 cut(s)	974
PstNI	CAGNNNCTG	1 cut(s)	511
PsuI	RGATCY	1 cut(s)	221
PsyI	GACNNNGTC	1 cut(s)	179
RseI	CAYNNNNRTG	1 cut(s)	377
SalI	GTCGAC	1 cut(s)	182
SaqAI	TTAA	1 cut(s)	501
SatI	GCNGC	6 cut(s)	333, 463, 546, 690, 1060, 1077
Sau3AI	GATC	4 cut(s)	199, 221, 1041, 1123
SchI	GAGTC	1 cut(s)	517
ScrFI	CCNGG	4 cut(s)	164, 165, 347, 707
SduI	GDGCHC	2 cut(s)	647, 975
SfaNI	GCATC	3 cut(s)	330, 627, 978
SfoI	GGCGCC	1 cut(s)	943
Sfr274I	CTCGAG	1 cut(s)	974
SlaI	CTCGAG	1 cut(s)	974
SmaI	CCCGGG	1 cut(s)	165
SmiMI	CAYNNNNRTG	1 cut(s)	377
SmlI	CTYRAG	3 cut(s)	303, 974, 992
SmoI	CTYRAG	3 cut(s)	303, 974, 992
Sse9I	AATT	5 cut(s)	39, 74, 94, 1010, 1111
SsiI	CCGC	6 cut(s)	6, 110, 689, 801, 912, 1127
SspDI	GGCGCC	1 cut(s)	941
SspMI	CTAG	2 cut(s)	750, 929
StyD4I	CCNGG	4 cut(s)	162, 163, 345, 705
TaaI	ACNGT	5 cut(s)	134, 379, 416, 765, 868
TaiI	ACGT	3 cut(s)	183, 620, 1003
TaqI	TCGA	6 cut(s)	183, 286, 324, 451, 578, 975
TasI	AATT	5 cut(s)	39, 74, 94, 1010, 1111
TauI	GCSGC	1 cut(s)	692
TfiI	GAWTC	2 cut(s)	89, 103
Tru1I	TTAA	1 cut(s)	501
Tru9I	TTAA	1 cut(s)	501
TscAI	CASTG	3 cut(s)	160, 647, 873
TseI	GCWGC	5 cut(s)	332, 462, 545, 1059, 1076
TspDTI	ATGAA	4 cut(s)	42, 560, 800, 936
TspGWI	ACGGA	1 cut(s)	446
TspMI	CCCGGG	1 cut(s)	163
TspRI	CASTG	3 cut(s)	160, 647, 873
Tth111I	GACNNNGTC	1 cut(s)	179
VneI	GTGCAC	1 cut(s)	643
XapI	RAATTY	2 cut(s)	74, 1010
XceI	RCATGY	1 cut(s)	400
XcmI	CCANNNNNNNNNTGG	1 cut(s)	453
XhoI	CTCGAG	1 cut(s)	974
XmaI	CCCGGG	1 cut(s)	163
XmiI	GTMKAC	1 cut(s)	183
XspI	CTAG	2 cut(s)	750, 929

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.