Rw5G004540
MYB Family

Myb-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0015569
Physical Location & Seq
Forward (+)
4753680 .. 4755112
1433 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G004540.1

Sequence Viewer

Length: 777 bp
ATGGTGAGGAGTAGTACTCAGCAGTGTGAAGAGAAGAGACCTTTGAAGAAAGGGCCATGGAGTCCTGATGAGGATCATAAGTTGATAGCTTACATTCAAAGATATGGTATTTGGAATTGGAGTCAGATGCCTAAACCTGCCGGTCTGGCAAGGTCTGGGAAAAGTTGTAGACTTCGTTGGATGAATTACTTGAGGCCGGATATTAAGCGCGGAAACTTCAGCAAGGAAGAAGAGGAGACCATAATCAAGTTGCAACAGAATATGGGAAACAGATGGTCTGTAATTGCAGCAAAACTTCCTGGAAGAACTGACAATGAAATCAAGAATTACTGGCACACCCACTTGAAAAGGCACCTCAACAATAACAAAGCCAAAAGCGGCGAGAATACTTCATCTGAAACTGAAACCTTTCTTCTTGATAAGGAAGCTCCAAAGGCATCTGAAACTTCAAGAGAAAACTTCTCTTCATCAAGTTCTGGTATTGCTGATGAAGTTTATCACATACCCCAGATTCCAGAACAGCAGAGCATCTGTGAAACATTTGGTGAACTCCAAAGTTTGTGGACAGATTCTCAGGTGACATCAACAGCGGTGGAAAATTCTGAGGCTATGTTTACAGATTCTGCTGGATTTACATTATCAAGTGCTGCTGATCAAGTGTGGCTGCAGCAAGAGCCCATATTTCCATGTGGTTCCTATAGTGATGATGCTCATGTTCCCATGAGTGACTATTGGGTCAATTTATCAGTACAATCTGATGAAACAAATGGAATGTGA

Protein Analysis

258

Amino Acids

29.38

Weight (kDa)

5.46

Isoelectric Point (pI)

62.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 17 - 64 3e-14 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 20 - 80 4.9e-14 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 70 - 115 4.6e-17 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 73 - 122 1.3e-08 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015569)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03680
malus_domestica MD05G1327500.v1.1 MD10G1305700.v1.1
prunus_persica Prupe.4G034000_v2.0.a1
pyrus_communis pycom05g30170 pycom10g25800
rosa_chinensis RchiOBHm_Chr5g0005721
rosa_laevigata RLG00000031321
rosa_multiflora Rmu_co8433021.1_g000001
rosa_roxburghii Rroxscaffold_1G00070120
rosa_rugosa Rorug04G0417600
rosa_samantha Rh5AG045200 Rh5BG043900 Rh5CG052700 Rh5DG043700
rosa_wichuraiana Rw5G004540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 734
Acc36I ACCTGC 1 cut(s) 145
AccB1I GGYRCC 1 cut(s) 351
AccI GTMKAC 1 cut(s) 169
AccII CGCG 1 cut(s) 210
AciI CCGC 3 cut(s) 210, 378, 590
AclWI GGATC 1 cut(s) 81
AcsI RAATTY 1 cut(s) 598
AcuI CTGAAG 1 cut(s) 202
AfaI GTAC 2 cut(s) 16, 750
AgsI TTSAA 4 cut(s) 46, 98, 346, 450
AjnI CCWGG 1 cut(s) 298
AluBI AGCT 2 cut(s) 89, 428
AluI AGCT 2 cut(s) 89, 428
Alw26I GTCTC 2 cut(s) 31, 230
AlwI GGATC 1 cut(s) 81
AlwNI CAGNNNCTG 1 cut(s) 623
AoxI GGCC 2 cut(s) 53, 194
ApeKI GCWGC 4 cut(s) 287, 647, 664, 667
ApoI RAATTY 1 cut(s) 598
Asp700I GAANNNNTTC 1 cut(s) 408
AspLEI GCGC 1 cut(s) 210
AspS9I GGNCC 1 cut(s) 53
AsuHPI GGTGA 3 cut(s) 16, 557, 589
BanI GGYRCC 1 cut(s) 351
BanII GRGCYC 1 cut(s) 678
BbvI GCAGC 4 cut(s) 299, 634, 651, 679
BccI CCATC 1 cut(s) 267
BciT130I CCWGG 1 cut(s) 300
BclI TGATCA 1 cut(s) 652
BcoDI GTCTC 2 cut(s) 31, 230
BfmI CTRYAG 2 cut(s) 665, 697
BfuAI ACCTGC 1 cut(s) 145
BglI GCCNNNNNGGC 1 cut(s) 146
BisI GCNGC 5 cut(s) 288, 379, 648, 665, 668
BlsI GCNGC 5 cut(s) 289, 380, 649, 666, 669
BmcAI AGTACT 1 cut(s) 16
Bme1390I CCNGG 1 cut(s) 300
BmgT120I GGNCC 1 cut(s) 53
BmiI GGNNCC 2 cut(s) 353, 694
BmrFI CCNGG 1 cut(s) 300
BmsI GCATC 4 cut(s) 117, 446, 537, 697
BplI GAGNNNNNCTC 1 cut(s) 33
BpuEI CTTGAG 1 cut(s) 211
BsaBI GATNNNNATC 1 cut(s) 72
BsaI GGTCTC 2 cut(s) 31, 230
BsaJI CCNNGG 1 cut(s) 56
Bse118I RCCGGY 1 cut(s) 140
Bse1I ACTGG 1 cut(s) 335
Bse8I GATNNNNATC 1 cut(s) 72
BseBI CCWGG 1 cut(s) 300
BseDI CCNNGG 1 cut(s) 56
BseGI GGATG 1 cut(s) 186
BseJI GATNNNNATC 1 cut(s) 72
BseMII CTCAG 3 cut(s) 32, 587, 594
BseNI ACTGG 1 cut(s) 335
BseRI GAGGAG 2 cut(s) 22, 248
BseXI GCAGC 4 cut(s) 299, 634, 651, 679
Bsh1236I CGCG 1 cut(s) 210
BshFI GGCC 2 cut(s) 55, 196
BshNI GGYRCC 1 cut(s) 351
BsiSI CCGG 2 cut(s) 141, 197
BsmAI GTCTC 2 cut(s) 31, 230
BsnI GGCC 2 cut(s) 55, 196
Bso31I GGTCTC 2 cut(s) 31, 230
Bsp1286I GDGCHC 1 cut(s) 678
Bsp143I GATC 2 cut(s) 73, 652
Bsp19I CCATGG 1 cut(s) 56
BspACI CCGC 3 cut(s) 210, 378, 590
BspANI GGCC 2 cut(s) 55, 196
BspCNI CTCAG 3 cut(s) 31, 586, 595
BspFNI CGCG 1 cut(s) 210
BspLI GGNNCC 2 cut(s) 353, 694
BspMAI CTGCAG 1 cut(s) 669
BspMI ACCTGC 1 cut(s) 145
BspPI GGATC 1 cut(s) 81
BspT107I GGYRCC 1 cut(s) 351
BspTNI GGTCTC 2 cut(s) 31, 230
BsrFI RCCGGY 1 cut(s) 140
BsrI ACTGG 1 cut(s) 335
BssAI RCCGGY 1 cut(s) 140
BssECI CCNNGG 1 cut(s) 56
BssMI GATC 2 cut(s) 73, 652
BssT1I CCWWGG 1 cut(s) 56
Bst2UI CCWGG 1 cut(s) 300
Bst6I CTCTTC 4 cut(s) 24, 29, 225, 469
BstDEI CTNAG 3 cut(s) 18, 573, 603
BstDSI CCRYGG 1 cut(s) 56
BstF5I GGATG 1 cut(s) 186
BstFNI CGCG 1 cut(s) 210
BstHHI GCGC 1 cut(s) 210
BstKTI GATC 2 cut(s) 76, 655
BstMAI GTCTC 2 cut(s) 31, 230
BstMBI GATC 2 cut(s) 73, 652
BstMWI GCNNNNNNNGC 3 cut(s) 146, 434, 673
BstNI CCWGG 1 cut(s) 300
BstSCI CCNGG 1 cut(s) 298
BstSFI CTRYAG 2 cut(s) 665, 697
BstUI CGCG 1 cut(s) 210
BstV1I GCAGC 4 cut(s) 299, 634, 651, 679
BsuRI GGCC 2 cut(s) 55, 196
BtgI CCRYGG 1 cut(s) 56
BtsCI GGATG 1 cut(s) 186
BtsI GCAGTG 1 cut(s) 29
BtsIMutI CAGTG 1 cut(s) 29
BveI ACCTGC 1 cut(s) 145
CaiI CAGNNNCTG 1 cut(s) 623
CfoI GCGC 1 cut(s) 210
Cfr10I RCCGGY 1 cut(s) 140
Cfr13I GGNCC 1 cut(s) 53
Csp6I GTAC 2 cut(s) 15, 749
CspCI CAANNNNNGTGG 4 cut(s) 542, 573, 577, 608
CviAII CATG 4 cut(s) 57, 687, 713, 721
CviJI RGCY 8 cut(s) 55, 89, 196, 371, 428, 608, 664, 676
CviKI_1 RGCY 8 cut(s) 55, 89, 196, 371, 428, 608, 664, 676
CviQI GTAC 2 cut(s) 15, 749
DdeI CTNAG 3 cut(s) 18, 573, 603
DpnI GATC 2 cut(s) 75, 654
DpnII GATC 2 cut(s) 73, 652
DrdI GACNNNNNNGTC 1 cut(s) 734
DseDI GACNNNNNNGTC 1 cut(s) 734
Eam1104I CTCTTC 4 cut(s) 24, 29, 225, 469
EarI CTCTTC 4 cut(s) 24, 29, 225, 469
Eco130I CCWWGG 1 cut(s) 56
Eco24I GRGCYC 1 cut(s) 678
Eco31I GGTCTC 2 cut(s) 31, 230
Eco57I CTGAAG 1 cut(s) 202
EcoRII CCWGG 1 cut(s) 298
EcoT14I CCWWGG 1 cut(s) 56
EcoT38I GRGCYC 1 cut(s) 678
ErhI CCWWGG 1 cut(s) 56
FaeI CATG 4 cut(s) 60, 690, 716, 724
FatI CATG 4 cut(s) 56, 686, 712, 720
FbaI TGATCA 1 cut(s) 652
FblI GTMKAC 1 cut(s) 169
Fnu4HI GCNGC 5 cut(s) 288, 379, 648, 665, 668
FokI GGATG 1 cut(s) 193
FriOI GRGCYC 1 cut(s) 678
Fsp4HI GCNGC 5 cut(s) 288, 379, 648, 665, 668
GlaI GCGC 1 cut(s) 209
GluI GCNGC 5 cut(s) 288, 379, 648, 665, 668
HaeIII GGCC 2 cut(s) 55, 196
HapII CCGG 2 cut(s) 141, 197
HhaI GCGC 1 cut(s) 210
Hin1II CATG 4 cut(s) 60, 690, 716, 724
Hin6I GCGC 1 cut(s) 208
HinP1I GCGC 1 cut(s) 208
HinfI GANTC 5 cut(s) 61, 121, 511, 569, 620
HpaII CCGG 2 cut(s) 141, 197
HphI GGTGA 3 cut(s) 16, 557, 589
Hpy166II GTNNAC 4 cut(s) 170, 548, 564, 615
Hpy188I TCNGA 5 cut(s) 126, 397, 442, 604, 757
Hpy188III TCNNGA 5 cut(s) 65, 322, 416, 450, 515
Hpy8I GTNNAC 4 cut(s) 170, 548, 564, 615
HpyCH4V TGCA 3 cut(s) 253, 287, 667
HpyF10VI GCNNNNNNNGC 3 cut(s) 146, 434, 673
HpyF3I CTNAG 3 cut(s) 18, 573, 603
Hsp92II CATG 4 cut(s) 60, 690, 716, 724
HspAI GCGC 1 cut(s) 208
Ksp22I TGATCA 1 cut(s) 652
Kzo9I GATC 2 cut(s) 73, 652
LmnI GCTCC 1 cut(s) 433
Lsp1109I GCAGC 4 cut(s) 299, 634, 651, 679
LweI GCATC 4 cut(s) 117, 446, 537, 697
MaeIII GTNAC 2 cut(s) 577, 725
MalI GATC 2 cut(s) 75, 654
MboI GATC 2 cut(s) 73, 652
MboII GAAGA 8 cut(s) 41, 46, 58, 239, 242, 315, 404, 456
MhlI GDGCHC 1 cut(s) 678
MluCI AATT 6 cut(s) 115, 184, 282, 325, 598, 739
MlyI GAGTC 2 cut(s) 70, 130
MmeI TCCRAC 1 cut(s) 158
MnlI CCTC 5 cut(s) 64, 186, 226, 365, 598
MroXI GAANNNNTTC 1 cut(s) 408
MseI TTAA 1 cut(s) 204
MspA1I CMGCKG 1 cut(s) 590
MspI CCGG 2 cut(s) 141, 197
MspR9I CCNGG 1 cut(s) 300
MvaI CCWGG 1 cut(s) 300
MvnI CGCG 1 cut(s) 210
MwoI GCNNNNNNNGC 3 cut(s) 146, 434, 673
NcoI CCATGG 1 cut(s) 56
NdeII GATC 2 cut(s) 73, 652
NlaIII CATG 4 cut(s) 60, 690, 716, 724
NlaIV GGNNCC 2 cut(s) 353, 694
NmuCI GTSAC 2 cut(s) 577, 725
PdmI GAANNNNTTC 1 cut(s) 408
PfeI GAWTC 3 cut(s) 511, 569, 620
PfoI TCCNGGA 1 cut(s) 298
PkrI GCNGC 5 cut(s) 289, 380, 649, 666, 669
PleI GAGTC 2 cut(s) 69, 129
PpsI GAGTC 2 cut(s) 69, 129
Psp6I CCWGG 1 cut(s) 298
PspGI CCWGG 1 cut(s) 298
PspN4I GGNNCC 2 cut(s) 353, 694
PspPI GGNCC 1 cut(s) 53
PstI CTGCAG 1 cut(s) 669
PstNI CAGNNNCTG 1 cut(s) 623
RsaI GTAC 2 cut(s) 16, 750
RsaNI GTAC 2 cut(s) 15, 749
SaqAI TTAA 1 cut(s) 204
SatI GCNGC 5 cut(s) 288, 379, 648, 665, 668
Sau3AI GATC 2 cut(s) 73, 652
Sau96I GGNCC 1 cut(s) 53
ScaI AGTACT 1 cut(s) 16
SchI GAGTC 2 cut(s) 70, 130
ScrFI CCNGG 1 cut(s) 300
SduI GDGCHC 1 cut(s) 678
SetI ASST 8 cut(s) 43, 91, 139, 155, 357, 410, 430, 579
SfaNI GCATC 4 cut(s) 117, 446, 537, 697
SfcI CTRYAG 2 cut(s) 665, 697
SmlI CTYRAG 1 cut(s) 190
SmoI CTYRAG 1 cut(s) 190
Sse9I AATT 6 cut(s) 115, 184, 282, 325, 598, 739
SsiI CCGC 3 cut(s) 210, 378, 590
StyD4I CCNGG 1 cut(s) 298
StyI CCWWGG 1 cut(s) 56
TasI AATT 6 cut(s) 115, 184, 282, 325, 598, 739
TatI WGTACW 2 cut(s) 14, 748
TauI GCSGC 1 cut(s) 381
TfiI GAWTC 3 cut(s) 511, 569, 620
Tru1I TTAA 1 cut(s) 204
Tru9I TTAA 1 cut(s) 204
TscAI CASTG 1 cut(s) 29
TseFI GTSAC 2 cut(s) 577, 725
TseI GCWGC 4 cut(s) 287, 647, 664, 667
Tsp45I GTSAC 2 cut(s) 577, 725
TspDTI ATGAA 6 cut(s) 197, 330, 381, 456, 504, 774
TspRI CASTG 1 cut(s) 29
XapI RAATTY 1 cut(s) 598
XmiI GTMKAC 1 cut(s) 169
XmnI GAANNNNTTC 1 cut(s) 408
ZrmI AGTACT 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.