Rw5G027630

A Receptor for Ubiquitination Targets

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0017630
Physical Location & Seq
Reverse (-)
41004660 .. 41006038
1379 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G027630.1

Sequence Viewer

Length: 1248 bp
ATGACGGAAAACCAACTACCGGAAGACATCATAGTCTCCGACATCCTGGTCAAACTCCCCATCAAATCCTTAATCCGCTGCACTTGCGTCTCGAAACGGTGGCGTTTCCTTATCTCCGACCCCGAATTCGCCAAATCTCACTTCCAACTAGCTCGCCGCCACCACACCTTGACCCGCCGCCTCCTCCTCTACTGCTCCCCCTCCCAAGTCGAATCCCTTGACTTGGAAACGACGCCGTCTTCGTCTTCTCCGATCCGCAACCTCACCTGTAAACTGAACAACCAACCCGAATCGATCAAGCTCCTCGGCTCCTGCAACGGCCTGGTATGCGTCTGTACGGATTCTACTTTAAGCAGTTGTGATCTTTTCATCTGGAACCCATCAACTGGGTTCTTGCGTAATCTACCGTACCCATGTTTTTCCTCGGCAACACACACCGTTCTCTACGGCGGGTTCGGTTACTTGTCGGCCACGGACGACTACAAGGTCGTGGTGGCGGCGGACAATGTGTTCGACTACGGCGATAATGTGGTGGAGGTGAAGGTGTTGTCATGGAGAACAGGGGTTTGGAGAAAGATTGAAGTGCACCCTTTGTTGTCGTACACGTTGTACCCGGCGGCGGTTGTGGTGGGGGAGACGCTTCATTGGTTTCGGAACGATCGTTGGTTAGGGTCGGCGATTATTGGTTTTGATTTGGACAAGGAGGAGTTTAGGGAGATGCCGCCGCCGCCGCCGAGTTTTTGGCAGAGATATGATAGAGATTATGTTGGGTGTGTGGGTTGTTGGGGAGGAAGGCTACATGTGTTTCGATATACGGCTCGTGTTTGCGATGTTATGGAGTTATGGGTTAGGGGTGAGAATGGTGATGGTGAGTCGTGGACCGAGGTGTTCAAGTTGAAAATAGCCGATGAGCCTCGGAAGGCTGTGTTTCTGCGGCCGTTTTTGGCTGTGGGAAGTAGTGCAGTTGTGGCGAAAGTGACTAGGAACGGGACGATGGAGATGGTCAGGATTGAACCTAGAGATGAGGAACCGGTTGTGGATATGTTTAAGAGGGGGGTTGAGCCGGCAATGATTCTATATGAAGAGAGCCTGCACCGGCTTCCTGGTTGTGGTGCGGTGGAAGAAGAGAAGGCGGAAGAGATTCAAACCCCAATATTTGGTGCTCTGTTCTGGATTACTGATCACTGGTACTCTATTCTGGCTTTGGTTGGTTTTCTTATTTCTCTGTGCTATATACCTGTAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

415

Amino Acids

47.19

Weight (kDa)

5.8

Isoelectric Point (pI)

44.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 5 - 47 2.4e-07 F-box-like
F-box PF00646 5 - 43 1.7e-07 F-box domain
FBA_1 PF07734 73 - 304 2.8e-20 F-box associated beta propeller domain
FBA_3 PF08268 84 - 295 2.6e-14 F-box associated beta propeller domain
b-prop_At3g26010-like PF24750 95 - 235 4.3e-07 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017630)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24880
rosa_chinensis RchiOBHm_Chr5g0044501
rosa_laevigata RLG00000034276
rosa_multiflora Rmu_co8185722.1_g000001 Rmu_sc0011598.1_g000002 Rmu_ssc0000151.1_g000013
rosa_roxburghii Rroxscaffold_1G00036510
rosa_rugosa Rorug05G0214600
rosa_samantha Rh5BG306300 Rh5CG333200 Rh5DG315600
rosa_wichuraiana Rw5G027630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 32, 47, 485
AccB7I CCANNNNNTGG 2 cut(s) 386, 1157
AclWI GGATC 1 cut(s) 247
AcoI YGGCCR 2 cut(s) 468, 935
AcsI RAATTY 1 cut(s) 125
AcyI GRCGYC 1 cut(s) 233
AfaI GTAC 5 cut(s) 337, 410, 602, 611, 1190
AfiI CCNNNNNNNGG 6 cut(s) 19, 223, 386, 619, 1109, 1157
AflIII ACRYGT 2 cut(s) 603, 799
AgeI ACCGGT 1 cut(s) 1030
AgsI TTSAA 5 cut(s) 581, 892, 898, 1013, 1145
AjnI CCWGG 3 cut(s) 45, 321, 1102
AjuI GAANNNNNNNTTGG 4 cut(s) 125, 157, 550, 582
AloI GAACNNNNNNTCC 2 cut(s) 494, 526
AluBI AGCT 2 cut(s) 152, 301
AluI AGCT 2 cut(s) 152, 301
Alw21I GWGCWC 2 cut(s) 588, 1165
Alw26I GTCTC 3 cut(s) 40, 94, 629
Alw44I GTGCAC 1 cut(s) 584
AlwI GGATC 1 cut(s) 247
AoxI GGCC 3 cut(s) 319, 468, 935
ApaLI GTGCAC 1 cut(s) 584
ApeKI GCWGC 1 cut(s) 78
ApoI RAATTY 1 cut(s) 125
ArsI GACNNNNNNTTYG 6 cut(s) 110, 142, 223, 255, 494, 526
AsiGI ACCGGT 1 cut(s) 1030
Asp700I GAANNNNTTC 1 cut(s) 1140
AspS9I GGNCC 1 cut(s) 879
AsuC2I CCSGG 1 cut(s) 614
AsuHPI GGTGA 5 cut(s) 256, 550, 866, 875, 881
AvaII GGWCC 1 cut(s) 879
BaeGI GKGCMC 1 cut(s) 588
BauI CACGAG 1 cut(s) 819
BbsI GAAGAC 3 cut(s) 30, 231, 237
Bbv12I GWGCWC 2 cut(s) 588, 1165
BbvI GCAGC 1 cut(s) 65
BccI CCATC 5 cut(s) 68, 388, 860, 988, 994
BceAI ACGGC 6 cut(s) 220, 334, 463, 535, 831, 922
BciT130I CCWGG 3 cut(s) 47, 323, 1104
BclI TGATCA 1 cut(s) 1180
BcnI CCSGG 1 cut(s) 614
BcoDI GTCTC 3 cut(s) 40, 94, 629
BfaI CTAG 3 cut(s) 149, 981, 1017
Bme1390I CCNGG 4 cut(s) 47, 323, 614, 1104
Bme18I GGWCC 1 cut(s) 879
BmgT120I GGNCC 1 cut(s) 879
BmiI GGNNCC 3 cut(s) 310, 377, 1029
BmrFI CCNGG 4 cut(s) 47, 323, 614, 1104
BmrI ACTGGG 1 cut(s) 396
BmsI GCATC 1 cut(s) 708
BmuI ACTGGG 1 cut(s) 396
BpiI GAAGAC 3 cut(s) 30, 231, 237
BpuMI CCSGG 1 cut(s) 614
Bsa29I ATCGAT 1 cut(s) 293
BsaHI GRCGYC 1 cut(s) 233
BsaJI CCNNGG 5 cut(s) 304, 423, 471, 882, 914
BsaWI WCCGGW 2 cut(s) 19, 1030
Bsc4I CCNNNNNNNGG 6 cut(s) 19, 223, 386, 619, 1109, 1157
Bse118I RCCGGY 3 cut(s) 1030, 1063, 1095
Bse1I ACTGG 2 cut(s) 391, 1190
Bse3DI GCAATG 1 cut(s) 1074
BseBI CCWGG 3 cut(s) 47, 323, 1104
BseCI ATCGAT 1 cut(s) 293
BseDI CCNNGG 5 cut(s) 304, 423, 471, 882, 914
BseGI GGATG 1 cut(s) 42
BseLI CCNNNNNNNGG 6 cut(s) 19, 223, 386, 619, 1109, 1157
BseMI GCAATG 1 cut(s) 1074
BseNI ACTGG 2 cut(s) 391, 1190
BseRI GAGGAG 4 cut(s) 173, 176, 293, 719
BseSI GKGCMC 1 cut(s) 588
BseX3I CGGCCG 1 cut(s) 935
BseXI GCAGC 1 cut(s) 65
BsgI GTGCAG 3 cut(s) 64, 981, 1076
Bsh1285I CGRYCG 2 cut(s) 661, 938
BshFI GGCC 3 cut(s) 321, 470, 937
BshTI ACCGGT 1 cut(s) 1030
BshVI ATCGAT 1 cut(s) 293
BsiEI CGRYCG 2 cut(s) 661, 938
BsiHKAI GWGCWC 2 cut(s) 588, 1165
BsiSI CCGG 5 cut(s) 20, 614, 1031, 1064, 1096
BslFI GGGAC 1 cut(s) 1003
BslI CCNNNNNNNGG 6 cut(s) 19, 223, 386, 619, 1109, 1157
BsmAI GTCTC 3 cut(s) 40, 94, 629
BsmBI CGTCTC 2 cut(s) 94, 629
BsmFI GGGAC 1 cut(s) 1003
BsnI GGCC 3 cut(s) 321, 470, 937
Bsp1286I GDGCHC 2 cut(s) 588, 1165
Bsp143I GATC 5 cut(s) 252, 294, 361, 658, 1180
BspANI GGCC 3 cut(s) 321, 470, 937
BspDI ATCGAT 1 cut(s) 293
BspLI GGNNCC 3 cut(s) 310, 377, 1029
BspPI GGATC 1 cut(s) 247
BsrDI GCAATG 1 cut(s) 1074
BsrFI RCCGGY 3 cut(s) 1030, 1063, 1095
BsrI ACTGG 2 cut(s) 391, 1190
BssAI RCCGGY 3 cut(s) 1030, 1063, 1095
BssECI CCNNGG 5 cut(s) 304, 423, 471, 882, 914
BssMI GATC 5 cut(s) 252, 294, 361, 658, 1180
BssNI GRCGYC 1 cut(s) 233
BssSI CACGAG 1 cut(s) 819
Bst2BI CACGAG 1 cut(s) 819
Bst2UI CCWGG 3 cut(s) 47, 323, 1104
Bst4CI ACNGT 3 cut(s) 99, 408, 439
Bst6I CTCTTC 3 cut(s) 1077, 1119, 1131
BstACI GRCGYC 1 cut(s) 233
BstC8I GCNNGC 3 cut(s) 154, 1065, 1091
BstDSI CCRYGG 1 cut(s) 471
BstF5I GGATG 1 cut(s) 42
BstKTI GATC 5 cut(s) 255, 297, 364, 661, 1183
BstMAI GTCTC 3 cut(s) 40, 94, 629
BstMBI GATC 5 cut(s) 252, 294, 361, 658, 1180
BstMCI CGRYCG 2 cut(s) 661, 938
BstMWI GCNNNNNNNGC 5 cut(s) 84, 327, 727, 730, 968
BstNI CCWGG 3 cut(s) 47, 323, 1104
BstNSI RCATGY 1 cut(s) 803
BstSCI CCNGG 4 cut(s) 45, 321, 612, 1102
BstSLI GKGCMC 1 cut(s) 588
BstV1I GCAGC 1 cut(s) 65
BstV2I GAAGAC 3 cut(s) 30, 231, 237
BstZI CGGCCG 1 cut(s) 935
Bsu15I ATCGAT 1 cut(s) 293
BsuRI GGCC 3 cut(s) 321, 470, 937
BsuTUI ATCGAT 1 cut(s) 293
BtgI CCRYGG 1 cut(s) 471
BtgZI GCGATG 1 cut(s) 843
BtsCI GGATG 1 cut(s) 42
BtsIMutI CAGTG 1 cut(s) 1183
Cac8I GCNNGC 3 cut(s) 154, 1065, 1091
Cfr10I RCCGGY 3 cut(s) 1030, 1063, 1095
Cfr13I GGNCC 1 cut(s) 879
ClaI ATCGAT 1 cut(s) 293
CseI GACGC 4 cut(s) 76, 241, 319, 646
Csp6I GTAC 5 cut(s) 336, 409, 601, 610, 1189
CspAI ACCGGT 1 cut(s) 1030
CviAII CATG 3 cut(s) 414, 552, 800
CviQI GTAC 5 cut(s) 336, 409, 601, 610, 1189
DpnI GATC 5 cut(s) 254, 296, 363, 660, 1182
DpnII GATC 5 cut(s) 252, 294, 361, 658, 1180
DrdI GACNNNNNNGTC 3 cut(s) 32, 47, 485
DseDI GACNNNNNNGTC 3 cut(s) 32, 47, 485
EaeI YGGCCR 2 cut(s) 468, 935
EagI CGGCCG 1 cut(s) 935
Eam1104I CTCTTC 3 cut(s) 1077, 1119, 1131
EarI CTCTTC 3 cut(s) 1077, 1119, 1131
EciI GGCGGA 2 cut(s) 515, 1148
EclXI CGGCCG 1 cut(s) 935
Eco47I GGWCC 1 cut(s) 879
Eco52I CGGCCG 1 cut(s) 935
EcoRI GAATTC 1 cut(s) 125
EcoRII CCWGG 3 cut(s) 45, 321, 1102
Esp3I CGTCTC 2 cut(s) 94, 629
FaeI CATG 3 cut(s) 417, 555, 803
FaqI GGGAC 1 cut(s) 1003
FatI CATG 3 cut(s) 413, 551, 799
FauI CCCGC 2 cut(s) 182, 443
FbaI TGATCA 1 cut(s) 1180
FokI GGATG 1 cut(s) 29
FspBI CTAG 3 cut(s) 149, 981, 1017
HaeIII GGCC 3 cut(s) 321, 470, 937
HapII CCGG 5 cut(s) 20, 614, 1031, 1064, 1096
HgaI GACGC 4 cut(s) 76, 241, 319, 646
Hin1I GRCGYC 1 cut(s) 233
Hin1II CATG 3 cut(s) 417, 555, 803
HinfI GANTC 6 cut(s) 212, 290, 341, 872, 1072, 1141
HpaII CCGG 5 cut(s) 20, 614, 1031, 1064, 1096
HphI GGTGA 5 cut(s) 256, 550, 866, 875, 881
Hpy166II GTNNAC 4 cut(s) 272, 586, 603, 879
Hpy188I TCNGA 5 cut(s) 40, 118, 252, 654, 918
Hpy188III TCNNGA 4 cut(s) 91, 373, 1006, 1171
Hpy8I GTNNAC 4 cut(s) 272, 586, 603, 879
Hpy99I CGWCG 1 cut(s) 235
HpyAV CCTTC 4 cut(s) 535, 786, 913, 1123
HpyCH4III ACNGT 3 cut(s) 99, 408, 439
HpyCH4IV ACGT 1 cut(s) 605
HpyCH4V TGCA 5 cut(s) 81, 315, 586, 962, 1093
HpyF10VI GCNNNNNNNGC 5 cut(s) 84, 327, 727, 730, 968
HpySE526I ACGT 1 cut(s) 605
Hsp92I GRCGYC 1 cut(s) 233
Hsp92II CATG 3 cut(s) 417, 555, 803
KroI GCCGGC 1 cut(s) 1063
KroNI GCCGGC 1 cut(s) 1065
Ksp22I TGATCA 1 cut(s) 1180
Kzo9I GATC 5 cut(s) 252, 294, 361, 658, 1180
LmnI GCTCC 3 cut(s) 200, 306, 314
Lsp1109I GCAGC 1 cut(s) 65
LweI GCATC 1 cut(s) 708
MaeI CTAG 3 cut(s) 149, 981, 1017
MaeII ACGT 1 cut(s) 605
MaeIII GTNAC 2 cut(s) 458, 976
MalI GATC 5 cut(s) 254, 296, 363, 660, 1182
MboI GATC 5 cut(s) 252, 294, 361, 658, 1180
MboII GAAGA 7 cut(s) 35, 231, 237, 1094, 1133, 1136, 1148
MhlI GDGCHC 2 cut(s) 588, 1165
MluCI AATT 1 cut(s) 125
MlyI GAGTC 1 cut(s) 881
MmeI TCCRAC 3 cut(s) 63, 141, 169
MroNI GCCGGC 1 cut(s) 1063
MroXI GAANNNNTTC 1 cut(s) 1140
MseI TTAA 3 cut(s) 71, 350, 1047
MspA1I CMGCKG 1 cut(s) 78
MspI CCGG 5 cut(s) 20, 614, 1031, 1064, 1096
MspR9I CCNGG 4 cut(s) 47, 323, 614, 1104
MvaI CCWGG 3 cut(s) 47, 323, 1104
MwoI GCNNNNNNNGC 5 cut(s) 84, 327, 727, 730, 968
NaeI GCCGGC 1 cut(s) 1065
NciI CCSGG 1 cut(s) 614
NdeII GATC 5 cut(s) 252, 294, 361, 658, 1180
NgoMIV GCCGGC 1 cut(s) 1063
NlaIII CATG 3 cut(s) 417, 555, 803
NlaIV GGNNCC 3 cut(s) 310, 377, 1029
NmeAIII GCCGAG 3 cut(s) 285, 404, 759
NmuCI GTSAC 1 cut(s) 976
NspI RCATGY 1 cut(s) 803
PciI ACATGT 1 cut(s) 799
PcsI WCGNNNNNNNCGW 4 cut(s) 239, 248, 519, 658
PdiI GCCGGC 1 cut(s) 1065
PdmI GAANNNNTTC 1 cut(s) 1140
PfeI GAWTC 5 cut(s) 212, 290, 341, 1072, 1141
PflFI GACNNNGTC 1 cut(s) 235
PflMI CCANNNNNTGG 2 cut(s) 386, 1157
PinAI ACCGGT 1 cut(s) 1030
Ple19I CGATCG 1 cut(s) 661
PleI GAGTC 1 cut(s) 880
PpsI GAGTC 1 cut(s) 880
PscI ACATGT 1 cut(s) 799
Psp6I CCWGG 3 cut(s) 45, 321, 1102
PspGI CCWGG 3 cut(s) 45, 321, 1102
PspN4I GGNNCC 3 cut(s) 310, 377, 1029
PspPI GGNCC 1 cut(s) 879
PsyI GACNNNGTC 1 cut(s) 235
PvuI CGATCG 1 cut(s) 661
RsaI GTAC 5 cut(s) 337, 410, 602, 611, 1190
RsaNI GTAC 5 cut(s) 336, 409, 601, 610, 1189
SaqAI TTAA 3 cut(s) 71, 350, 1047
Sau3AI GATC 5 cut(s) 252, 294, 361, 658, 1180
Sau96I GGNCC 1 cut(s) 879
SchI GAGTC 1 cut(s) 881
ScrFI CCNGG 4 cut(s) 47, 323, 614, 1104
SduI GDGCHC 2 cut(s) 588, 1165
SfaNI GCATC 1 cut(s) 708
SinI GGWCC 1 cut(s) 879
Sse9I AATT 1 cut(s) 125
SspI AATATT 1 cut(s) 1155
SspMI CTAG 3 cut(s) 149, 981, 1017
StyD4I CCNGG 4 cut(s) 45, 321, 612, 1102
TaaI ACNGT 3 cut(s) 99, 408, 439
TaiI ACGT 1 cut(s) 608
TaqI TCGA 5 cut(s) 92, 210, 293, 513, 808
TaqII GACCGA 1 cut(s) 896
TasI AATT 1 cut(s) 125
TauI GCSGC 9 cut(s) 159, 180, 500, 620, 724, 727, 730, 733, 937
TfiI GAWTC 5 cut(s) 212, 290, 341, 1072, 1141
Tru1I TTAA 3 cut(s) 71, 350, 1047
Tru9I TTAA 3 cut(s) 71, 350, 1047
TscAI CASTG 1 cut(s) 1190
TseFI GTSAC 1 cut(s) 976
TseI GCWGC 1 cut(s) 78
Tsp45I GTSAC 1 cut(s) 976
TspDTI ATGAA 3 cut(s) 358, 632, 1095
TspGWI ACGGA 3 cut(s) 20, 353, 488
TspRI CASTG 1 cut(s) 1190
Tth111I GACNNNGTC 1 cut(s) 235
Van91I CCANNNNNTGG 2 cut(s) 386, 1157
VneI GTGCAC 1 cut(s) 584
VpaK11BI GGWCC 1 cut(s) 879
XapI RAATTY 1 cut(s) 125
XceI RCATGY 1 cut(s) 803
XmnI GAANNNNTTC 1 cut(s) 1140
XspI CTAG 3 cut(s) 149, 981, 1017
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.