Rw5G028250

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0018616
Physical Location & Seq
Reverse (-)
42650570 .. 42664288
13719 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G028250.1

Sequence Viewer

Length: 510 bp
ATGGAGGCAACAAATGGTAGATTGTCTCCTAGAGGCGTGCGGCTAGCTACTTCATGGCTTTTCATCCTCTTCATATTTTGGGCGAATCCAAGCGAGAGCAGCACCCAAGAGAACGCCGCCTGCATCGTGCAGTGTCTGAGGCCAACCAAGGCTGTGATCCTCCTCCAGCGGCAGGGCTTCATCGTCCTGGTCGTCGACTACACTGGCTCGTCGCCGAAATCAACGAGTATCCGAGCAAGGAAAGTCGAGGTGCACGCTGCTGGTTATGTCGTCTTGGAAACTGAACGGGTGCTCGTGAAACAGAGGCCTGATAACCTTTTCGGAATCTCAGTTCCAACTAAGGGGTGTTTCTACGCGCTCTTAGCAGAAGCGAACCAGCTTCACAACAGTGTTACTACTGTGCTTAACGTACTGTGGAGACCTTTGTTCAATTTGGAACTAATGTCTAGCCCACAAGCACACAATTCTGTACCGCCTGGAATTCAATTGACGGCTACTGAAAAAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

169

Amino Acids

18.69

Weight (kDa)

9.55

Isoelectric Point (pI)

46.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018616)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0045481
rosa_laevigata RLG00000034364
rosa_samantha Rh5AG264400 Rh5AG304300 Rh5BG269400 Rh5BG312500 Rh5CG303000 Rh5CG339400 Rh5CG342500
rosa_wichuraiana Rw5G028250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 195
AccII CGCG 1 cut(s) 356
AciI CCGC 4 cut(s) 40, 117, 169, 473
AclWI GGATC 1 cut(s) 151
AcsI RAATTY 1 cut(s) 480
AfaI GTAC 2 cut(s) 411, 471
AfiI CCNNNNNNNGG 2 cut(s) 172, 341
AgsI TTSAA 2 cut(s) 430, 485
AjnI CCWGG 2 cut(s) 186, 475
AleI CACNNNNGTG 1 cut(s) 387
AloI GAACNNNNNNTCC 2 cut(s) 315, 347
AluBI AGCT 2 cut(s) 47, 379
AluI AGCT 2 cut(s) 47, 379
Alw21I GWGCWC 2 cut(s) 255, 294
Alw26I GTCTC 2 cut(s) 30, 412
Alw44I GTGCAC 1 cut(s) 251
AlwI GGATC 1 cut(s) 151
AlwNI CAGNNNCTG 1 cut(s) 136
AoxI GGCC 2 cut(s) 140, 305
ApaLI GTGCAC 1 cut(s) 251
ApeKI GCWGC 2 cut(s) 99, 257
ApoI RAATTY 1 cut(s) 480
AspLEI GCGC 1 cut(s) 358
AsuNHI GCTAGC 1 cut(s) 43
BaeGI GKGCMC 1 cut(s) 255
BauI CACGAG 1 cut(s) 293
Bbv12I GWGCWC 2 cut(s) 255, 294
BbvI GCAGC 2 cut(s) 111, 244
BceAI ACGGC 1 cut(s) 507
BciT130I CCWGG 2 cut(s) 188, 477
BciVI GTATCC 1 cut(s) 239
BcoDI GTCTC 2 cut(s) 30, 412
BfaI CTAG 3 cut(s) 30, 44, 447
BfuI GTATCC 1 cut(s) 239
BisI GCNGC 5 cut(s) 41, 100, 117, 170, 258
BlsI GCNGC 5 cut(s) 42, 101, 118, 171, 259
Bme1390I CCNGG 2 cut(s) 188, 477
BmrFI CCNGG 2 cut(s) 188, 477
BmsI GCATC 1 cut(s) 132
BmtI GCTAGC 1 cut(s) 47
BpmI CTGGAG 1 cut(s) 149
BsaI GGTCTC 1 cut(s) 412
BsaJI CCNNGG 1 cut(s) 147
Bsc4I CCNNNNNNNGG 2 cut(s) 172, 341
Bse1I ACTGG 1 cut(s) 208
BseBI CCWGG 2 cut(s) 188, 477
BseDI CCNNGG 1 cut(s) 147
BseGI GGATG 1 cut(s) 63
BseLI CCNNNNNNNGG 2 cut(s) 172, 341
BseMII CTCAG 2 cut(s) 128, 342
BseNI ACTGG 1 cut(s) 208
BseRI GAGGAG 1 cut(s) 152
BseSI GKGCMC 1 cut(s) 255
BseXI GCAGC 2 cut(s) 111, 244
BsgI GTGCAG 1 cut(s) 149
Bsh1236I CGCG 1 cut(s) 356
BshFI GGCC 2 cut(s) 142, 307
BsiHKAI GWGCWC 2 cut(s) 255, 294
BslI CCNNNNNNNGG 2 cut(s) 172, 341
BsmAI GTCTC 2 cut(s) 30, 412
BsnI GGCC 2 cut(s) 142, 307
Bso31I GGTCTC 1 cut(s) 412
Bsp1286I GDGCHC 2 cut(s) 255, 294
Bsp143I GATC 1 cut(s) 156
BspACI CCGC 4 cut(s) 40, 117, 169, 473
BspANI GGCC 2 cut(s) 142, 307
BspCNI CTCAG 2 cut(s) 129, 341
BspFNI CGCG 1 cut(s) 356
BspOI GCTAGC 1 cut(s) 47
BspPI GGATC 1 cut(s) 151
BspTNI GGTCTC 1 cut(s) 412
BsrI ACTGG 1 cut(s) 208
BssECI CCNNGG 1 cut(s) 147
BssMI GATC 1 cut(s) 156
BssSI CACGAG 1 cut(s) 293
BssT1I CCWWGG 1 cut(s) 147
Bst2BI CACGAG 1 cut(s) 293
Bst2UI CCWGG 2 cut(s) 188, 477
Bst4CI ACNGT 3 cut(s) 389, 400, 414
Bst6I CTCTTC 1 cut(s) 74
BstC8I GCNNGC 4 cut(s) 38, 45, 121, 255
BstDEI CTNAG 4 cut(s) 137, 328, 339, 361
BstF5I GGATG 1 cut(s) 63
BstFNI CGCG 1 cut(s) 356
BstHHI GCGC 1 cut(s) 358
BstKTI GATC 1 cut(s) 159
BstMAI GTCTC 2 cut(s) 30, 412
BstMBI GATC 1 cut(s) 156
BstMWI GCNNNNNNNGC 2 cut(s) 99, 362
BstNI CCWGG 2 cut(s) 188, 477
BstSCI CCNGG 2 cut(s) 186, 475
BstSLI GKGCMC 1 cut(s) 255
BstUI CGCG 1 cut(s) 356
BstV1I GCAGC 2 cut(s) 111, 244
BsuI GTATCC 1 cut(s) 239
BsuRI GGCC 2 cut(s) 142, 307
BtsCI GGATG 1 cut(s) 63
BtsI GCAGTG 1 cut(s) 137
BtsIMutI CAGTG 3 cut(s) 137, 201, 394
Cac8I GCNNGC 4 cut(s) 38, 45, 121, 255
CaiI CAGNNNCTG 1 cut(s) 136
CfoI GCGC 1 cut(s) 358
Csp6I GTAC 2 cut(s) 410, 470
CviAII CATG 1 cut(s) 54
CviQI GTAC 2 cut(s) 410, 470
DdeI CTNAG 4 cut(s) 137, 328, 339, 361
DpnI GATC 1 cut(s) 158
DpnII GATC 1 cut(s) 156
Eam1104I CTCTTC 1 cut(s) 74
EarI CTCTTC 1 cut(s) 74
Eco130I CCWWGG 1 cut(s) 147
Eco147I AGGCCT 1 cut(s) 307
Eco31I GGTCTC 1 cut(s) 412
EcoRI GAATTC 1 cut(s) 480
EcoRII CCWGG 2 cut(s) 186, 475
EcoT14I CCWWGG 1 cut(s) 147
ErhI CCWWGG 1 cut(s) 147
FaeI CATG 1 cut(s) 57
FaiI YATR 3 cut(s) 55, 74, 267
FatI CATG 1 cut(s) 53
FblI GTMKAC 1 cut(s) 195
Fnu4HI GCNGC 5 cut(s) 41, 100, 117, 170, 258
FokI GGATG 1 cut(s) 50
Fsp4HI GCNGC 5 cut(s) 41, 100, 117, 170, 258
FspBI CTAG 3 cut(s) 30, 44, 447
GlaI GCGC 1 cut(s) 357
GluI GCNGC 5 cut(s) 41, 100, 117, 170, 258
GsuI CTGGAG 1 cut(s) 149
HaeIII GGCC 2 cut(s) 142, 307
HhaI GCGC 1 cut(s) 358
Hin1II CATG 1 cut(s) 57
Hin6I GCGC 1 cut(s) 356
HinP1I GCGC 1 cut(s) 356
HincII GTYRAC 1 cut(s) 196
HindII GTYRAC 1 cut(s) 196
HinfI GANTC 2 cut(s) 85, 324
Hpy166II GTNNAC 2 cut(s) 196, 253
Hpy188I TCNGA 3 cut(s) 138, 233, 323
Hpy188III TCNNGA 1 cut(s) 295
Hpy8I GTNNAC 2 cut(s) 196, 253
Hpy99I CGWCG 2 cut(s) 197, 214
HpyCH4III ACNGT 3 cut(s) 389, 400, 414
HpyCH4IV ACGT 1 cut(s) 408
HpyCH4V TGCA 3 cut(s) 123, 130, 253
HpyF10VI GCNNNNNNNGC 2 cut(s) 99, 362
HpyF3I CTNAG 4 cut(s) 137, 328, 339, 361
HpySE526I ACGT 1 cut(s) 408
Hsp92II CATG 1 cut(s) 57
HspAI GCGC 1 cut(s) 356
Kzo9I GATC 1 cut(s) 156
Lsp1109I GCAGC 2 cut(s) 111, 244
LweI GCATC 1 cut(s) 132
MaeI CTAG 3 cut(s) 30, 44, 447
MaeII ACGT 1 cut(s) 408
MaeIII GTNAC 1 cut(s) 391
MalI GATC 1 cut(s) 158
MboI GATC 1 cut(s) 156
MboII GAAGA 1 cut(s) 61
MfeI CAATTG 1 cut(s) 485
MhlI GDGCHC 2 cut(s) 255, 294
MluCI AATT 4 cut(s) 430, 463, 480, 485
MmeI TCCRAC 1 cut(s) 359
MnlI CCTC 7 cut(s) 26, 77, 132, 170, 173, 241, 297
MseI TTAA 1 cut(s) 405
MslI CAYNNNNRTG 1 cut(s) 387
MspA1I CMGCKG 1 cut(s) 169
MspR9I CCNGG 2 cut(s) 188, 477
MunI CAATTG 1 cut(s) 485
MvaI CCWGG 2 cut(s) 188, 477
MvnI CGCG 1 cut(s) 356
MwoI GCNNNNNNNGC 2 cut(s) 99, 362
NdeII GATC 1 cut(s) 156
NheI GCTAGC 1 cut(s) 43
NlaIII CATG 1 cut(s) 57
OliI CACNNNNGTG 1 cut(s) 387
PceI AGGCCT 1 cut(s) 307
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PfeI GAWTC 2 cut(s) 85, 324
PkrI GCNGC 5 cut(s) 42, 101, 118, 171, 259
Psp6I CCWGG 2 cut(s) 186, 475
PspGI CCWGG 2 cut(s) 186, 475
PstNI CAGNNNCTG 1 cut(s) 136
RsaI GTAC 2 cut(s) 411, 471
RsaNI GTAC 2 cut(s) 410, 470
RseI CAYNNNNRTG 1 cut(s) 387
SalI GTCGAC 1 cut(s) 194
SaqAI TTAA 1 cut(s) 405
SatI GCNGC 5 cut(s) 41, 100, 117, 170, 258
Sau3AI GATC 1 cut(s) 156
ScrFI CCNGG 2 cut(s) 188, 477
SduI GDGCHC 2 cut(s) 255, 294
SetI ASST 6 cut(s) 49, 252, 318, 381, 411, 424
SfaNI GCATC 1 cut(s) 132
SmiMI CAYNNNNRTG 1 cut(s) 387
Sse9I AATT 4 cut(s) 430, 463, 480, 485
SseBI AGGCCT 1 cut(s) 307
SsiI CCGC 4 cut(s) 40, 117, 169, 473
SspMI CTAG 3 cut(s) 30, 44, 447
StuI AGGCCT 1 cut(s) 307
StyD4I CCNGG 2 cut(s) 186, 475
StyI CCWWGG 1 cut(s) 147
TaaI ACNGT 3 cut(s) 389, 400, 414
TaiI ACGT 1 cut(s) 411
TaqI TCGA 2 cut(s) 195, 246
TasI AATT 4 cut(s) 430, 463, 480, 485
TauI GCSGC 3 cut(s) 43, 119, 172
TfiI GAWTC 2 cut(s) 85, 324
Tru1I TTAA 1 cut(s) 405
Tru9I TTAA 1 cut(s) 405
TscAI CASTG 3 cut(s) 137, 208, 394
TseI GCWGC 2 cut(s) 99, 257
TspDTI ATGAA 4 cut(s) 42, 52, 61, 169
TspRI CASTG 3 cut(s) 137, 208, 394
VneI GTGCAC 1 cut(s) 251
XapI RAATTY 1 cut(s) 480
XmiI GTMKAC 1 cut(s) 195
XspI CTAG 3 cut(s) 30, 44, 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.