Rw6G021310

Ubiquitin domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0010891
Physical Location & Seq
Forward (+)
42519519 .. 42523633
4115 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G021310.1

Sequence Viewer

Length: 1701 bp
ATGGGGGTTGAGGGCGATTCAAGCGAGTCTAAAGTAGGTGGTGGAGAAGGCGGAGAGCTGATTAATATTCGGTGCTCTAATGGGTCCAAGTTTTCGGTCAGGGCGGCTTTGGATATCGATGTTGGGGCATTCAAGGCGATTTTGGCTCAGAATTGTGATATTCCAGCTGATCAGCAAAGGTTGATTTACAAAGGTCGGATTTTGAAGGACGACCAGACCCTCACAAGTTATGGTTTGCAGGCAGATCATACTGTCCACATGGTTCGTGCCTTTGCTCCTGGTGCCTCAACAACACCGGCTGCTCCCGCCTCTAATGCTACTTCTGCAAGTCCCAACACTGCAGCTGCAGCTGGTGTTACTCGTGGTGTTGGTTCCGAGGGGGTTGAGAATCCCGACCTTGGGGCATCTTTGCTATTTGGGCAGGGTTTGGGTGGTCTTGGTAGTGGTGGGGCCCCTGGCAGTTTATTTGGGAGTGGGCTTCCGGAATTTGAACAAGTGCAGCAGCAACTAACTCAGAACCCGAACATGATGAGGGATATAATGAATACGCCTGCCATTCAGAGTCTTATGAATAACCCTGAGTTGATGCGAGGCTTGATCATGAACAATCCACAAATGCGTGACATCATTGACCGGAACCCAGAGCTTGCACATATACTTAATGATCCTGGTATTCTTAGACAGACACTAGAAGCTGCAAGGAACCCTGAACTCATGCGTGAGATGATGCGAAACACTGACAGGGCAATGAGTAATATTGAATCTTCGCCTGAGGGGTTCAACATGCTGCGACGAATGTATGAAAATGTTCAGGAGCCATTTTTGAACGCTACCACAATGACTGGGAATGCTGGGAATGATGCGGCTGCAAACCCCTTTGCTGCTCTTTTAGGGAATCAGGGTGGGGCACAGGCCAGGGATGGGACTAACAACCCTTCAACCACCGGTTCAGAAACTACTGCAGGTTCTGCTATTCCTAATGCAAACCCTCTTCCCAACCCTTGGAGCAATACTACTGGAGGAAATCAAACAAACGCACCCAGGTCAACTCCTGCTGGGGATGGGAGGGCACCAAGTATTGGCGGTCTTGGAGGTCTGGGAGGTCTAGGAGGTCTGGGAGGTCTTGGTGGTCTTCCGGGAATGGAACAAATGATGGGTGGCATGCCAGACGCTAATGCAATGAATCAGCTCATGCAGAATCCAGCAATATCACAGATGATGCAAAGCATGCTCTCTAATCCCCAATATATGAATCAGATCCTGAACCTCAATCCACAACTACGCGCCATGGTTGATTTAAACCCTCAACTTAGAGAAATGATGCAAAATCCAGAGCTTCTACGTCAGTTGACAAACCCTGAGACAATGCAGCAAATGCTTGCATTGCAGCAGTCACTAGTTTCTCAGACCAATCGACAGCAATCAACTCAGGATCCAACTCAGACTGGTGGTGCTACAGGAACCCCTAATAATGGTGCTGGACTGGAGATGCTGATGAACATGTTTGGTGGTCTTGGAGCTGGTAGCTTGGCTGCACCAGTCAGCAATGTACCACCGGAGGAACGTTATGCAACTCAGCTGTCACAACTTCAAGAAATGGGATTCTTCGATACTCAAGAGAATATCAGGGCATTGCAAGCCACTTCCGGGAATGTCCATGCTGCAGTAGAGCGACTTTTAGGGAACCCACCAGGAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000045 GO:0001666 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005773 GO:0005776 GO:0005783 GO:0005829 GO:0005886 GO:0006508 GO:0006511 GO:0006807 GO:0006914 GO:0006950 GO:0006996 GO:0007033 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009628 GO:0009893 GO:0009894 GO:0009896 GO:0009966 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0010604 GO:0010646 GO:0010941 GO:0010959 GO:0012505 GO:0016020 GO:0016043 GO:0016234 GO:0016235 GO:0016236 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0019941 GO:0022411 GO:0022607 GO:0022898 GO:0023051 GO:0030162 GO:0030163 GO:0030433 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031593 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032409 GO:0032410 GO:0032412 GO:0032413 GO:0032434 GO:0032436 GO:0032879 GO:0032984 GO:0032991 GO:0033554 GO:0034762 GO:0034763 GO:0034765 GO:0034766 GO:0034976 GO:0035973 GO:0036293 GO:0036294 GO:0036503 GO:0042176 GO:0042221 GO:0042802 GO:0042981 GO:0043067 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043269 GO:0043271 GO:0043632 GO:0043933 GO:0044085 GO:0044092 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051603 GO:0051716 GO:0051924 GO:0051926 GO:0060255 GO:0061136 GO:0061912 GO:0061919 GO:0065007 GO:0065009 GO:0070013 GO:0070482 GO:0070887 GO:0070925 GO:0071453 GO:0071456 GO:0071704 GO:0071840 GO:0071944 GO:0080090 GO:0080134 GO:0080135 GO:0097352 GO:0140030 GO:1900407 GO:1901019 GO:1901020 GO:1901339 GO:1901340 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901800 GO:1902175 GO:1902531 GO:1902882 GO:1903050 GO:1903052 GO:1903069 GO:1903071 GO:1903169 GO:1903170 GO:1903201 GO:1903320 GO:1903322 GO:1903362 GO:1903364 GO:1904062 GO:1904063 GO:1904292 GO:1904294 GO:1905037 GO:1905897 GO:1905898 GO:2000058 GO:2000060 GO:2001233 GO:2001242 GO:2001257 GO:2001258
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
UBA ubiquitin

Protein Analysis

566

Amino Acids

59.34

Weight (kDa)

4.73

Isoelectric Point (pI)

41.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 21 - 89 8.1e-19 Ubiquitin family
Rad60-SLD PF11976 21 - 88 1.4e-06 Ubiquitin-2 like Rad60 SUMO-like
UBQLN1 PF23195 169 - 294 3.1e-51 Ubiquilin-1-like domain
UBQLN1 PF23195 391 - 468 1.7e-11 Ubiquilin-1-like domain
UBA PF00627 523 - 559 1.3e-08 UBA/TS-N domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010891)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G17190 AT2G17200
fragaria_vesca FvH4_2g16610 FvH4_2g16610
malus_domestica MD05G1134300.v1.1 MD10G1139400.v1.1
prunus_persica Prupe.8G178100_v2.0.a1
pyrus_communis pycom05g13090 pycom10g11840
rosa_chinensis RchiOBHm_Chr6g0280671
rosa_laevigata RLG00000013037
rosa_roxburghii Rroxscaffold_7G00187770
rosa_rugosa Rorug06G0133800 Rorug06G0133800 Rorug06G0133800
rosa_samantha Rh6AG246300 Rh6BG248500 Rh6CG249800 Rh6DG241200
rosa_wichuraiana Rw6G021310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 953
AccB1I GGYRCC 2 cut(s) 281, 1069
AccB7I CCANNNNNTGG 2 cut(s) 1002, 1079
AccII CGCG 1 cut(s) 1284
AccIII TCCGGA 1 cut(s) 481
AciI CCGC 5 cut(s) 51, 104, 306, 863, 1083
AclI AACGTT 1 cut(s) 1564
AclWI GGATC 4 cut(s) 659, 1252, 1427, 1440
AcsI RAATTY 1 cut(s) 485
AfaI GTAC 1 cut(s) 1551
AfiI CCNNNNNNNGG 7 cut(s) 398, 399, 921, 1002, 1079, 1472, 1647
AflIII ACRYGT 1 cut(s) 1500
AgeI ACCGGT 1 cut(s) 944
AgsI TTSAA 9 cut(s) 21, 133, 205, 491, 761, 781, 826, 939, 1592
AhlI ACTAGT 1 cut(s) 1396
AjnI CCWGG 6 cut(s) 277, 454, 667, 914, 1040, 1690
Alw21I GWGCWC 1 cut(s) 77
Alw26I GTCTC 1 cut(s) 1355
AlwI GGATC 4 cut(s) 659, 1252, 1427, 1440
AlwNI CAGNNNCTG 2 cut(s) 968, 1261
Aor13HI TCCGGA 1 cut(s) 481
AoxI GGCC 2 cut(s) 450, 912
ApaI GGGCCC 1 cut(s) 454
ApoI RAATTY 1 cut(s) 485
AseI ATTAAT 1 cut(s) 63
AsiGI ACCGGT 1 cut(s) 944
Asp700I GAANNNNTTC 1 cut(s) 807
AspLEI GCGC 1 cut(s) 1286
AspS9I GGNCC 3 cut(s) 84, 450, 451
AsuC2I CCSGG 2 cut(s) 1137, 1648
AvaII GGWCC 1 cut(s) 84
AxyI CCTNAGG 1 cut(s) 771
BaeGI GKGCMC 3 cut(s) 454, 910, 1072
BamHI GGATCC 1 cut(s) 1432
BanI GGYRCC 2 cut(s) 281, 1069
BanII GRGCYC 1 cut(s) 454
BauI CACGAG 1 cut(s) 360
BbsI GAAGAC 1 cut(s) 1124
Bbv12I GWGCWC 1 cut(s) 77
BccI CCATC 3 cut(s) 914, 1055, 1147
BciT130I CCWGG 6 cut(s) 279, 456, 669, 916, 1042, 1692
BclI TGATCA 2 cut(s) 169, 597
BcnI CCSGG 2 cut(s) 1137, 1648
BcoDI GTCTC 1 cut(s) 1355
BcuI ACTAGT 1 cut(s) 1396
BfaI CTAG 3 cut(s) 689, 1106, 1397
BfmI CTRYAG 5 cut(s) 339, 345, 960, 1455, 1662
BfuAI ACCTGC 1 cut(s) 953
Bme1390I CCNGG 8 cut(s) 279, 456, 669, 916, 1042, 1137, 1648, 1692
Bme18I GGWCC 1 cut(s) 84
BmgT120I GGNCC 3 cut(s) 84, 450, 451
BmrFI CCNGG 8 cut(s) 279, 456, 669, 916, 1042, 1137, 1648, 1692
BmrI ACTGGG 1 cut(s) 852
BmsI GCATC 7 cut(s) 413, 576, 717, 850, 1209, 1311, 1479
BmuI ACTGGG 1 cut(s) 852
BpiI GAAGAC 1 cut(s) 1124
BpmI CTGGAG 2 cut(s) 1038, 1505
BpuEI CTTGAG 1 cut(s) 1599
BpuMI CCSGG 2 cut(s) 1137, 1648
Bsa29I ATCGAT 1 cut(s) 117
BsaJI CCNNGG 7 cut(s) 375, 397, 454, 915, 1001, 1040, 1287
BsaWI WCCGGW 4 cut(s) 481, 633, 944, 1555
Bsc4I CCNNNNNNNGG 7 cut(s) 398, 399, 921, 1002, 1079, 1472, 1647
Bse118I RCCGGY 2 cut(s) 295, 944
Bse1I ACTGG 5 cut(s) 847, 1021, 1450, 1488, 1538
Bse21I CCTNAGG 1 cut(s) 771
Bse3DI GCAATG 5 cut(s) 753, 1185, 1382, 1552, 1631
BseAI TCCGGA 1 cut(s) 481
BseBI CCWGG 6 cut(s) 279, 456, 669, 916, 1042, 1692
BseCI ATCGAT 1 cut(s) 117
BseDI CCNNGG 7 cut(s) 375, 397, 454, 915, 1001, 1040, 1287
BseGI GGATG 2 cut(s) 925, 1066
BseLI CCNNNNNNNGG 7 cut(s) 398, 399, 921, 1002, 1079, 1472, 1647
BseMI GCAATG 5 cut(s) 753, 1185, 1382, 1552, 1631
BseMII CTCAG 9 cut(s) 161, 527, 570, 762, 1350, 1418, 1442, 1454, 1589
BseNI ACTGG 5 cut(s) 847, 1021, 1450, 1488, 1538
BseSI GKGCMC 3 cut(s) 454, 910, 1072
BseYI CCCAGC 2 cut(s) 851, 1055
BsgI GTGCAG 2 cut(s) 518, 1518
Bsh1236I CGCG 1 cut(s) 1284
BshFI GGCC 2 cut(s) 452, 914
BshNI GGYRCC 2 cut(s) 281, 1069
BshTI ACCGGT 1 cut(s) 944
BshVI ATCGAT 1 cut(s) 117
BsiHKAI GWGCWC 1 cut(s) 77
BsiSI CCGG 7 cut(s) 296, 482, 634, 945, 1136, 1556, 1647
BslFI GGGAC 2 cut(s) 315, 937
BslI CCNNNNNNNGG 7 cut(s) 398, 399, 921, 1002, 1079, 1472, 1647
BsmAI GTCTC 1 cut(s) 1355
BsmFI GGGAC 2 cut(s) 315, 937
BsmI GAATGC 2 cut(s) 128, 853
BsnI GGCC 2 cut(s) 452, 914
Bsp120I GGGCCC 1 cut(s) 450
Bsp1286I GDGCHC 4 cut(s) 77, 454, 910, 1072
Bsp13I TCCGGA 1 cut(s) 481
Bsp143I GATC 6 cut(s) 169, 244, 597, 664, 1257, 1432
Bsp19I CCATGG 1 cut(s) 1287
BspACI CCGC 5 cut(s) 51, 104, 306, 863, 1083
BspANI GGCC 2 cut(s) 452, 914
BspCNI CTCAG 9 cut(s) 160, 526, 571, 763, 1351, 1417, 1441, 1453, 1588
BspDI ATCGAT 1 cut(s) 117
BspEI TCCGGA 1 cut(s) 481
BspFNI CGCG 1 cut(s) 1284
BspHI TCATGA 1 cut(s) 600
BspMAI CTGCAG 4 cut(s) 343, 349, 964, 1666
BspMI ACCTGC 1 cut(s) 953
BspPI GGATC 4 cut(s) 659, 1252, 1427, 1440
BspT107I GGYRCC 2 cut(s) 281, 1069
BsrDI GCAATG 5 cut(s) 753, 1185, 1382, 1552, 1631
BsrFI RCCGGY 2 cut(s) 295, 944
BsrI ACTGG 5 cut(s) 847, 1021, 1450, 1488, 1538
BssAI RCCGGY 2 cut(s) 295, 944
BssECI CCNNGG 7 cut(s) 375, 397, 454, 915, 1001, 1040, 1287
BssMI GATC 6 cut(s) 169, 244, 597, 664, 1257, 1432
BssSI CACGAG 1 cut(s) 360
BssT1I CCWWGG 3 cut(s) 397, 1001, 1287
Bst2BI CACGAG 1 cut(s) 360
Bst2UI CCWGG 6 cut(s) 279, 456, 669, 916, 1042, 1692
Bst4CI ACNGT 1 cut(s) 253
Bst6I CTCTTC 1 cut(s) 996
BstAPI GCANNNNNTGC 3 cut(s) 968, 1228, 1375
BstC8I GCNNGC 7 cut(s) 240, 552, 648, 1163, 1229, 1380, 1638
BstDSI CCRYGG 1 cut(s) 1287
BstF5I GGATG 2 cut(s) 925, 1066
BstFNI CGCG 1 cut(s) 1284
BstHHI GCGC 1 cut(s) 1286
BstKTI GATC 6 cut(s) 172, 247, 600, 667, 1260, 1435
BstMAI GTCTC 1 cut(s) 1355
BstMBI GATC 6 cut(s) 169, 244, 597, 664, 1257, 1432
BstNI CCWGG 6 cut(s) 279, 456, 669, 916, 1042, 1692
BstNSI RCATGY 4 cut(s) 787, 1165, 1231, 1504
BstSCI CCNGG 8 cut(s) 277, 454, 667, 914, 1040, 1135, 1646, 1690
BstSFI CTRYAG 5 cut(s) 339, 345, 960, 1455, 1662
BstSLI GKGCMC 3 cut(s) 454, 910, 1072
BstUI CGCG 1 cut(s) 1284
BstV2I GAAGAC 1 cut(s) 1124
BstX2I RGATCY 2 cut(s) 1257, 1432
BstYI RGATCY 2 cut(s) 1257, 1432
Bsu15I ATCGAT 1 cut(s) 117
Bsu36I CCTNAGG 1 cut(s) 771
BsuRI GGCC 2 cut(s) 452, 914
BsuTUI ATCGAT 1 cut(s) 117
BtgI CCRYGG 1 cut(s) 1287
BtsCI GGATG 2 cut(s) 925, 1066
BtsI GCAGTG 1 cut(s) 336
BtsIMutI CAGTG 2 cut(s) 336, 735
BveI ACCTGC 1 cut(s) 953
Cac8I GCNNGC 7 cut(s) 240, 552, 648, 1163, 1229, 1380, 1638
CaiI CAGNNNCTG 2 cut(s) 968, 1261
CciI TCATGA 1 cut(s) 600
CfoI GCGC 1 cut(s) 1286
Cfr10I RCCGGY 2 cut(s) 295, 944
Cfr13I GGNCC 3 cut(s) 84, 450, 451
ClaI ATCGAT 1 cut(s) 117
CseI GACGC 1 cut(s) 1178
Csp6I GTAC 1 cut(s) 1550
CspAI ACCGGT 1 cut(s) 944
CviQI GTAC 1 cut(s) 1550
DpnI GATC 6 cut(s) 171, 246, 599, 666, 1259, 1434
DpnII GATC 6 cut(s) 169, 244, 597, 664, 1257, 1432
DraI TTTAAA 1 cut(s) 1299
Eam1104I CTCTTC 1 cut(s) 996
EarI CTCTTC 1 cut(s) 996
EciI GGCGGA 1 cut(s) 66
Eco130I CCWWGG 3 cut(s) 397, 1001, 1287
Eco24I GRGCYC 1 cut(s) 454
Eco32I GATATC 1 cut(s) 115
Eco47I GGWCC 1 cut(s) 84
Eco81I CCTNAGG 1 cut(s) 771
EcoO109I RGGNCCY 2 cut(s) 450, 451
EcoRII CCWGG 6 cut(s) 277, 454, 667, 914, 1040, 1690
EcoRV GATATC 1 cut(s) 115
EcoT14I CCWWGG 3 cut(s) 397, 1001, 1287
EcoT38I GRGCYC 1 cut(s) 454
ErhI CCWWGG 3 cut(s) 397, 1001, 1287
FaqI GGGAC 2 cut(s) 315, 937
FauI CCCGC 1 cut(s) 313
FbaI TGATCA 2 cut(s) 169, 597
FokI GGATG 2 cut(s) 932, 1073
FriOI GRGCYC 1 cut(s) 454
FspBI CTAG 3 cut(s) 689, 1106, 1397
GlaI GCGC 1 cut(s) 1285
GsaI CCCAGC 2 cut(s) 855, 1059
GsuI CTGGAG 2 cut(s) 1038, 1505
HaeIII GGCC 2 cut(s) 452, 914
HapII CCGG 7 cut(s) 296, 482, 634, 945, 1136, 1556, 1647
HgaI GACGC 1 cut(s) 1178
HhaI GCGC 1 cut(s) 1286
Hin6I GCGC 1 cut(s) 1284
HinP1I GCGC 1 cut(s) 1284
HincII GTYRAC 2 cut(s) 1047, 1350
HindII GTYRAC 2 cut(s) 1047, 1350
HpaII CCGG 7 cut(s) 296, 482, 634, 945, 1136, 1556, 1647
Hpy166II GTNNAC 3 cut(s) 256, 1047, 1350
Hpy188I TCNGA 9 cut(s) 150, 198, 376, 516, 561, 952, 1257, 1407, 1443
Hpy188III TCNNGA 9 cut(s) 392, 482, 601, 812, 1261, 1331, 1430, 1592, 1616
Hpy8I GTNNAC 3 cut(s) 256, 1047, 1350
Hpy99I CGWCG 1 cut(s) 795
HpyAV CCTTC 3 cut(s) 41, 199, 945
HpyCH4III ACNGT 1 cut(s) 253
HpyCH4IV ACGT 2 cut(s) 1342, 1564
HpySE526I ACGT 2 cut(s) 1342, 1564
HspAI GCGC 1 cut(s) 1284
Kpn2I TCCGGA 1 cut(s) 481
Ksp22I TGATCA 2 cut(s) 169, 597
Kzo9I GATC 6 cut(s) 169, 244, 597, 664, 1257, 1432
LmnI GCTCC 6 cut(s) 280, 307, 814, 1005, 1517, 1694
LweI GCATC 7 cut(s) 413, 576, 717, 850, 1209, 1311, 1479
MaeI CTAG 3 cut(s) 689, 1106, 1397
MaeII ACGT 2 cut(s) 1342, 1564
MaeIII GTNAC 4 cut(s) 355, 620, 1392, 1581
MalI GATC 6 cut(s) 171, 246, 599, 666, 1259, 1434
MboI GATC 6 cut(s) 169, 244, 597, 664, 1257, 1432
MboII GAAGA 4 cut(s) 756, 983, 1124, 1597
MflI RGATCY 2 cut(s) 1257, 1432
MhlI GDGCHC 4 cut(s) 77, 454, 910, 1072
MluCI AATT 2 cut(s) 151, 485
MlyI GAGTC 2 cut(s) 35, 571
MmeI TCCRAC 2 cut(s) 176, 1460
MroI TCCGGA 1 cut(s) 481
MroXI GAANNNNTTC 1 cut(s) 807
MseI TTAA 3 cut(s) 63, 660, 1298
MspA1I CMGCKG 4 cut(s) 167, 344, 350, 1579
MspI CCGG 7 cut(s) 296, 482, 634, 945, 1136, 1556, 1647
MspR9I CCNGG 8 cut(s) 279, 456, 669, 916, 1042, 1137, 1648, 1692
Mva1269I GAATGC 2 cut(s) 128, 853
MvaI CCWGG 6 cut(s) 279, 456, 669, 916, 1042, 1692
MvnI CGCG 1 cut(s) 1284
NciI CCSGG 2 cut(s) 1137, 1648
NcoI CCATGG 1 cut(s) 1287
NdeII GATC 6 cut(s) 169, 244, 597, 664, 1257, 1432
NmuCI GTSAC 3 cut(s) 620, 1392, 1581
NspI RCATGY 4 cut(s) 787, 1165, 1231, 1504
PaeI GCATGC 2 cut(s) 1165, 1231
PagI TCATGA 1 cut(s) 600
PciI ACATGT 1 cut(s) 1500
PctI GAATGC 2 cut(s) 128, 853
PdmI GAANNNNTTC 1 cut(s) 807
PfeI GAWTC 8 cut(s) 17, 388, 761, 895, 1183, 1198, 1252, 1602
PflMI CCANNNNNTGG 2 cut(s) 1002, 1079
PfoI TCCNGGA 2 cut(s) 1135, 1646
PinAI ACCGGT 1 cut(s) 944
PleI GAGTC 2 cut(s) 34, 570
PpsI GAGTC 2 cut(s) 34, 570
PscI ACATGT 1 cut(s) 1500
PshBI ATTAAT 1 cut(s) 63
Psp1406I AACGTT 1 cut(s) 1564
Psp6I CCWGG 6 cut(s) 277, 454, 667, 914, 1040, 1690
PspFI CCCAGC 2 cut(s) 851, 1055
PspGI CCWGG 6 cut(s) 277, 454, 667, 914, 1040, 1690
PspOMI GGGCCC 1 cut(s) 450
PspPI GGNCC 3 cut(s) 84, 450, 451
PstI CTGCAG 4 cut(s) 343, 349, 964, 1666
PstNI CAGNNNCTG 2 cut(s) 968, 1261
PsuI RGATCY 2 cut(s) 1257, 1432
PvuII CAGCTG 4 cut(s) 167, 344, 350, 1579
RsaI GTAC 1 cut(s) 1551
RsaNI GTAC 1 cut(s) 1550
SaqAI TTAA 3 cut(s) 63, 660, 1298
Sau3AI GATC 6 cut(s) 169, 244, 597, 664, 1257, 1432
Sau96I GGNCC 3 cut(s) 84, 450, 451
SchI GAGTC 2 cut(s) 35, 571
ScrFI CCNGG 8 cut(s) 279, 456, 669, 916, 1042, 1137, 1648, 1692
SduI GDGCHC 4 cut(s) 77, 454, 910, 1072
SfaNI GCATC 7 cut(s) 413, 576, 717, 850, 1209, 1311, 1479
SfcI CTRYAG 5 cut(s) 339, 345, 960, 1455, 1662
SinI GGWCC 1 cut(s) 84
SmlI CTYRAG 1 cut(s) 1614
SmoI CTYRAG 1 cut(s) 1614
SpeI ACTAGT 1 cut(s) 1396
SphI GCATGC 2 cut(s) 1165, 1231
Sse9I AATT 2 cut(s) 151, 485
SsiI CCGC 5 cut(s) 51, 104, 306, 863, 1083
SspI AATATT 2 cut(s) 67, 757
SspMI CTAG 3 cut(s) 689, 1106, 1397
StyD4I CCNGG 8 cut(s) 277, 454, 667, 914, 1040, 1135, 1646, 1690
StyI CCWWGG 3 cut(s) 397, 1001, 1287
TaaI ACNGT 1 cut(s) 253
TaiI ACGT 2 cut(s) 1345, 1567
TaqI TCGA 3 cut(s) 117, 1414, 1608
TaqII GACCGA 1 cut(s) 85
TasI AATT 2 cut(s) 151, 485
TauI GCSGC 2 cut(s) 107, 866
TfiI GAWTC 8 cut(s) 17, 388, 761, 895, 1183, 1198, 1252, 1602
Tru1I TTAA 3 cut(s) 63, 660, 1298
Tru9I TTAA 3 cut(s) 63, 660, 1298
TscAI CASTG 2 cut(s) 343, 742
TseFI GTSAC 3 cut(s) 620, 1392, 1581
Tsp45I GTSAC 3 cut(s) 620, 1392, 1581
TspDTI ATGAA 7 cut(s) 557, 584, 617, 816, 1196, 1265, 1511
TspRI CASTG 2 cut(s) 343, 742
Van91I CCANNNNNTGG 2 cut(s) 1002, 1079
VpaK11BI GGWCC 1 cut(s) 84
VspI ATTAAT 1 cut(s) 63
XapI RAATTY 1 cut(s) 485
XceI RCATGY 4 cut(s) 787, 1165, 1231, 1504
XmnI GAANNNNTTC 1 cut(s) 807
XspI CTAG 3 cut(s) 689, 1106, 1397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.