Rw7G034270

Pentatricopeptide repeat-containing protein At1g03100

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0010496
Physical Location & Seq
Reverse (-)
50792825 .. 50794620
1796 bp
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UTR
Exon/CDS
Intron
Rw7G034270.1

Sequence Viewer

Length: 1617 bp
ATGCTTTCTGCGAGGAAATTCAGCCACGGGAGAGCTTCAGTTTGCCCTTGTGTTTTATATGTTGTACAAGCATCGTATGCTGGTTTAAGTTTAGCTGGGTTGTTTGAATCTCAGGTTCGAGCTTATTCAAACGTTCGGTATGTTTCTGGGTCTGTTACTTCTCATTGTTTGGCCAAGTCCATTGTCCAAGATTGTATTTTGGGATTACCCAAAAAGTCTATATCTGAAGTCGGTTCATTTTCGACTATGGCTGGAACAATCTTGGTCCAGGCTCGAGACCCGGCTAAGTTATATGTGGAGATACAAAATGCAGTTGATGAGCATAGACTAAATGATGCGTGGAAGCTTTATGAGCAGTATATGCAGATGGATGGGTTTCCTAGGAAATCTGTCGTTAATAAGCTTTTAGCGGGTTTTGTAGAAAGCTCTGATGTTCAGTGGCTTGAGAAGGCTTATGGGTTGGTAGAGCAGGCTATTGGGGAAAGCAAACAGAACTTGTTGGAGAGAAACACGCTGATTTATGTTTCTTTTGGTCTTGCGAGAGCTGGGTTACCAGTTCCTGCTTCGACTGTTTTGAGAAGAATGGTAGAAATGGAAGAATACCCTCCTGTTGCTGCTTGGTCTGCTGTCTTGGCCTACATGTCTCAAACAGCTCAAGGGGCTTATGTTGCTGCTGAGTTGATTCTTGAGATAGGTTACTTGTTCCAAGATCACAGGATCGACGCACGTAAGAAGTGTAATGCACCTCTGATTGCTATGAAGCCTAATACTACTGCTTTCAACATTGCTTTGGCTGGTTGTCTCTTGTTTGGAACAACTAAGAAAGCAGAGCAGCTCCTTGACATGATGCCTCGAGTTGACACTGCATCTAACATGGTTTTGGAAGTACTGCGGAAAGCAAAAGAAGTAAGAGGTTCTCTTGCAGTAGCAACGCTGGTATTTGATGCTGCTGGAAGGGGAAGCAAGTTCCCTCATGGAGTTAATTCTGGATCCTGGCATTATTCAAGCCATGGAGAATCATATGCTTATGGAGACAATAGGTTACCTGGTAATCTTGTATTATCTTACAAAGAGTTTTCAAGGGATAGAAATTTCTTGAGACTTGAGGTTGAGGCTAAGGAAGTGCTGGGTGCATTGTTAGCTAAATTGCAGAGCCAAGTTGAATTGGTAACAACTGACCACAGTATCCTCCAGCCAACTGAAAAAATACTTGCGAAATTAGTCAAGGCCTTTCTAGAAGCAGACAAAACCAAGGATTTGGCTGCCTTTCTTATAAAGGCAGATAAAGAAGCTTCCCCAGCTTCCAGTGATGATTCAGCCTTGGTTCATGTCATTAATTCATGCATATCGCTTGGATGGTTAAACAAGGCACATGACCTTCTTGATGAAATGTGCTTGGCTGGTGTAAGAACTGGTTCTTCCGTATACGCCGCTCTTTTAAAAGCATATTGTGAAGCAAACCAAGCAGGAAATGCAGCATCACTATTAAGAGATGCTCGTAAGTCTGGTATCCAGCTAGATGCAAGCAGTTATGAGGCACTGATCCGATCGAAGGTGCTTCAAAATGATACACAGGGAGCTCTTCATTTGTACACAGAGAGGTTGAGAGGTTGGTGA

Protein Analysis

538

Amino Acids

58.89

Weight (kDa)

8.4

Isoelectric Point (pI)

34.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_At1g68980 PF25245 93 - 234 3.3e-10 At1g68980, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1274
AccBSI CCGCTC 1 cut(s) 1433
AccI GTMKAC 1 cut(s) 1425
AciI CCGC 3 cut(s) 410, 892, 1431
AclI AACGTT 1 cut(s) 132
AclWI GGATC 4 cut(s) 725, 984, 997, 1537
AcoI YGGCCR 1 cut(s) 171
AcsI RAATTY 2 cut(s) 17, 1090
AcuI CTGAAG 2 cut(s) 21, 246
AfaI GTAC 3 cut(s) 66, 888, 1592
AfiI CCNNNNNNNGG 1 cut(s) 1552
AflIII ACRYGT 1 cut(s) 639
AgsI TTSAA 7 cut(s) 107, 129, 781, 1005, 1080, 1163, 1562
AjnI CCWGG 3 cut(s) 267, 992, 1045
Alw21I GWGCWC 1 cut(s) 1582
Alw26I GTCTC 5 cut(s) 270, 648, 806, 1026, 1093
AlwI GGATC 4 cut(s) 725, 984, 997, 1537
AlwNI CAGNNNCTG 1 cut(s) 560
Ama87I CYCGRG 2 cut(s) 273, 852
AoxI GGCC 3 cut(s) 171, 633, 1227
ApeKI GCWGC 6 cut(s) 614, 671, 832, 947, 1262, 1475
ApoI RAATTY 2 cut(s) 17, 1090
AseI ATTAAT 1 cut(s) 1335
Asp700I GAANNNNTTC 1 cut(s) 1414
AspA2I CCTAGG 1 cut(s) 380
AspS9I GGNCC 1 cut(s) 265
AsuC2I CCSGG 1 cut(s) 281
AvaI CYCGRG 2 cut(s) 273, 852
AvaII GGWCC 1 cut(s) 265
AvrII CCTAGG 1 cut(s) 380
BaeI ACNNNNGTAYC 2 cut(s) 1168, 1201
BalI TGGCCA 1 cut(s) 173
BamHI GGATCC 1 cut(s) 989
BanII GRGCYC 1 cut(s) 1582
Bbv12I GWGCWC 1 cut(s) 1582
BbvI GCAGC 6 cut(s) 601, 658, 844, 934, 1249, 1487
BccI CCATC 3 cut(s) 361, 365, 1350
BciT130I CCWGG 3 cut(s) 269, 994, 1047
BciVI GTATCC 2 cut(s) 1196, 1520
BcnI CCSGG 1 cut(s) 281
BcoDI GTCTC 5 cut(s) 270, 648, 806, 1026, 1093
BfaI CTAG 3 cut(s) 381, 1235, 1517
BfuI GTATCC 2 cut(s) 1196, 1520
BisI GCNGC 7 cut(s) 615, 672, 833, 948, 1263, 1431, 1476
BlnI CCTAGG 1 cut(s) 380
BlsI GCNGC 7 cut(s) 616, 673, 834, 949, 1264, 1432, 1477
BmcAI AGTACT 1 cut(s) 888
Bme1390I CCNGG 4 cut(s) 269, 281, 994, 1047
Bme18I GGWCC 1 cut(s) 265
BmeT110I CYCGRG 2 cut(s) 273, 852
BmgT120I GGNCC 1 cut(s) 265
BmiI GGNNCC 1 cut(s) 991
BmrFI CCNGG 4 cut(s) 269, 281, 994, 1047
BmsI GCATC 8 cut(s) 80, 325, 837, 875, 934, 1483, 1487, 1510
BpmI CTGGAG 1 cut(s) 1175
Bpu10I CCTNAGC 1 cut(s) 1116
BpuEI CTTGAG 5 cut(s) 464, 639, 707, 1117, 1124
BpuMI CCSGG 1 cut(s) 281
BsaAI YACGTR 1 cut(s) 728
BsaI GGTCTC 1 cut(s) 270
BsaJI CCNNGG 5 cut(s) 25, 380, 1009, 1251, 1320
Bsc4I CCNNNNNNNGG 1 cut(s) 1552
Bse1I ACTGG 3 cut(s) 554, 1305, 1417
Bse3DI GCAATG 1 cut(s) 783
BseBI CCWGG 3 cut(s) 269, 994, 1047
BseDI CCNNGG 5 cut(s) 25, 380, 1009, 1251, 1320
BseGI GGATG 2 cut(s) 376, 1361
BseLI CCNNNNNNNGG 1 cut(s) 1552
BseMI GCAATG 1 cut(s) 783
BseMII CTCAG 2 cut(s) 125, 666
BseNI ACTGG 3 cut(s) 554, 1305, 1417
BseXI GCAGC 6 cut(s) 601, 658, 844, 934, 1249, 1487
BseYI CCCAGC 4 cut(s) 95, 545, 1126, 1297
Bsh1285I CGRYCG 1 cut(s) 1550
BshFI GGCC 3 cut(s) 173, 635, 1229
BsiEI CGRYCG 1 cut(s) 1550
BsiHKAI GWGCWC 1 cut(s) 1582
BsiHKCI CYCGRG 2 cut(s) 273, 852
BsiSI CCGG 1 cut(s) 281
BslI CCNNNNNNNGG 1 cut(s) 1552
BsmAI GTCTC 5 cut(s) 270, 648, 806, 1026, 1093
BsnI GGCC 3 cut(s) 173, 635, 1229
Bso31I GGTCTC 1 cut(s) 270
BsoBI CYCGRG 2 cut(s) 273, 852
Bsp1286I GDGCHC 1 cut(s) 1582
Bsp1407I TGTACA 2 cut(s) 64, 1590
Bsp143I GATC 5 cut(s) 709, 717, 989, 1542, 1547
Bsp19I CCATGG 1 cut(s) 1009
BspACI CCGC 3 cut(s) 410, 892, 1431
BspANI GGCC 3 cut(s) 173, 635, 1229
BspCNI CTCAG 2 cut(s) 124, 667
BspLI GGNNCC 1 cut(s) 991
BspPI GGATC 4 cut(s) 725, 984, 997, 1537
BspQI GCTCTTC 1 cut(s) 1587
BspTNI GGTCTC 1 cut(s) 270
BsrBI CCGCTC 1 cut(s) 1433
BsrDI GCAATG 1 cut(s) 783
BsrGI TGTACA 2 cut(s) 64, 1590
BsrI ACTGG 3 cut(s) 554, 1305, 1417
BssECI CCNNGG 5 cut(s) 25, 380, 1009, 1251, 1320
BssMI GATC 5 cut(s) 709, 717, 989, 1542, 1547
BssNAI GTATAC 1 cut(s) 1426
BssT1I CCWWGG 4 cut(s) 380, 1009, 1251, 1320
Bst1107I GTATAC 1 cut(s) 1426
Bst2UI CCWGG 3 cut(s) 269, 994, 1047
Bst4CI ACNGT 2 cut(s) 571, 1184
Bst6I CTCTTC 1 cut(s) 1587
BstAPI GCANNNNNTGC 3 cut(s) 77, 361, 1472
BstAUI TGTACA 2 cut(s) 64, 1590
BstBAI YACGTR 1 cut(s) 728
BstC8I GCNNGC 2 cut(s) 471, 1525
BstDEI CTNAG 5 cut(s) 111, 285, 675, 819, 1116
BstDSI CCRYGG 2 cut(s) 25, 1009
BstEII GGTNACC 2 cut(s) 549, 1041
BstF5I GGATG 2 cut(s) 376, 1361
BstKTI GATC 5 cut(s) 712, 720, 992, 1545, 1550
BstMAI GTCTC 5 cut(s) 270, 648, 806, 1026, 1093
BstMBI GATC 5 cut(s) 709, 717, 989, 1542, 1547
BstMCI CGRYCG 1 cut(s) 1550
BstNI CCWGG 3 cut(s) 269, 994, 1047
BstNSI RCATGY 1 cut(s) 643
BstPI GGTNACC 2 cut(s) 549, 1041
BstSCI CCNGG 4 cut(s) 267, 279, 992, 1045
BstV1I GCAGC 6 cut(s) 601, 658, 844, 934, 1249, 1487
BstX2I RGATCY 1 cut(s) 989
BstXI CCANNNNNNTGG 1 cut(s) 1258
BstYI RGATCY 1 cut(s) 989
BstZ17I GTATAC 1 cut(s) 1426
BsuI GTATCC 2 cut(s) 1196, 1520
BsuRI GGCC 3 cut(s) 173, 635, 1229
BtgI CCRYGG 2 cut(s) 25, 1009
BtsCI GGATG 2 cut(s) 376, 1361
BtsI GCAGTG 1 cut(s) 861
BtsIMutI CAGTG 4 cut(s) 443, 861, 1312, 1538
Cac8I GCNNGC 2 cut(s) 471, 1525
CaiI CAGNNNCTG 1 cut(s) 560
Cfr13I GGNCC 1 cut(s) 265
CseI GACGC 1 cut(s) 731
CsiI ACCWGGT 1 cut(s) 1045
Csp6I GTAC 3 cut(s) 65, 887, 1591
CviAII CATG 8 cut(s) 640, 844, 874, 974, 1010, 1328, 1341, 1373
CviQI GTAC 3 cut(s) 65, 887, 1591
DdeI CTNAG 5 cut(s) 111, 285, 675, 819, 1116
DpnI GATC 5 cut(s) 711, 719, 991, 1544, 1549
DpnII GATC 5 cut(s) 709, 717, 989, 1542, 1547
DraI TTTAAA 1 cut(s) 1440
EaeI YGGCCR 1 cut(s) 171
Eam1104I CTCTTC 1 cut(s) 1587
EarI CTCTTC 1 cut(s) 1587
Ecl136II GAGCTC 1 cut(s) 1580
Eco130I CCWWGG 4 cut(s) 380, 1009, 1251, 1320
Eco147I AGGCCT 1 cut(s) 1229
Eco24I GRGCYC 1 cut(s) 1582
Eco31I GGTCTC 1 cut(s) 270
Eco47I GGWCC 1 cut(s) 265
Eco53kI GAGCTC 1 cut(s) 1580
Eco57I CTGAAG 2 cut(s) 21, 246
Eco88I CYCGRG 2 cut(s) 273, 852
Eco91I GGTNACC 2 cut(s) 549, 1041
EcoICRI GAGCTC 1 cut(s) 1580
EcoO65I GGTNACC 2 cut(s) 549, 1041
EcoRII CCWGG 3 cut(s) 267, 992, 1045
EcoT14I CCWWGG 4 cut(s) 380, 1009, 1251, 1320
EcoT22I ATGCAT 1 cut(s) 1346
EcoT38I GRGCYC 1 cut(s) 1582
ErhI CCWWGG 4 cut(s) 380, 1009, 1251, 1320
FaeI CATG 8 cut(s) 643, 847, 877, 977, 1013, 1331, 1344, 1376
FatI CATG 8 cut(s) 639, 843, 873, 973, 1009, 1327, 1340, 1372
FauI CCCGC 1 cut(s) 403
FauNDI CATATG 1 cut(s) 1021
FblI GTMKAC 1 cut(s) 1425
Fnu4HI GCNGC 7 cut(s) 615, 672, 833, 948, 1263, 1431, 1476
FokI GGATG 2 cut(s) 383, 1368
FriOI GRGCYC 1 cut(s) 1582
Fsp4HI GCNGC 7 cut(s) 615, 672, 833, 948, 1263, 1431, 1476
FspBI CTAG 3 cut(s) 381, 1235, 1517
GluI GCNGC 7 cut(s) 615, 672, 833, 948, 1263, 1431, 1476
GsaI CCCAGC 4 cut(s) 99, 549, 1130, 1301
GsuI CTGGAG 1 cut(s) 1175
HaeIII GGCC 3 cut(s) 173, 635, 1229
HapII CCGG 1 cut(s) 281
HgaI GACGC 1 cut(s) 731
Hin1II CATG 8 cut(s) 643, 847, 877, 977, 1013, 1331, 1344, 1376
HincII GTYRAC 1 cut(s) 859
HindII GTYRAC 1 cut(s) 859
HindIII AAGCTT 3 cut(s) 344, 401, 1290
HinfI GANTC 4 cut(s) 107, 682, 1016, 1313
HpaII CCGG 1 cut(s) 281
Hpy166II GTNNAC 3 cut(s) 859, 1426, 1593
Hpy188I TCNGA 4 cut(s) 226, 430, 750, 1547
Hpy188III TCNNGA 6 cut(s) 275, 686, 987, 1096, 1235, 1382
Hpy8I GTNNAC 3 cut(s) 859, 1426, 1593
Hpy99I CGWCG 1 cut(s) 725
HpyAV CCTTC 4 cut(s) 442, 948, 1388, 1546
HpyCH4III ACNGT 2 cut(s) 571, 1184
HpyCH4IV ACGT 2 cut(s) 132, 727
HpyF3I CTNAG 5 cut(s) 111, 285, 675, 819, 1116
HpySE526I ACGT 2 cut(s) 132, 727
Hsp92II CATG 8 cut(s) 643, 847, 877, 977, 1013, 1331, 1344, 1376
Kzo9I GATC 5 cut(s) 709, 717, 989, 1542, 1547
LguI GCTCTTC 1 cut(s) 1587
LmnI GCTCC 2 cut(s) 840, 1577
Lsp1109I GCAGC 6 cut(s) 601, 658, 844, 934, 1249, 1487
LweI GCATC 8 cut(s) 80, 325, 837, 875, 934, 1483, 1487, 1510
MabI ACCWGGT 1 cut(s) 1045
MaeI CTAG 3 cut(s) 381, 1235, 1517
MaeII ACGT 2 cut(s) 132, 727
MaeIII GTNAC 5 cut(s) 154, 549, 695, 1041, 1168
MalI GATC 5 cut(s) 711, 719, 991, 1544, 1549
MbiI CCGCTC 1 cut(s) 1433
MboI GATC 5 cut(s) 709, 717, 989, 1542, 1547
MboII GAAGA 4 cut(s) 591, 608, 1410, 1574
MflI RGATCY 1 cut(s) 989
MhlI GDGCHC 1 cut(s) 1582
MlsI TGGCCA 1 cut(s) 173
MluCI AATT 7 cut(s) 17, 982, 1090, 1145, 1163, 1217, 1336
MluNI TGGCCA 1 cut(s) 173
MmeI TCCRAC 1 cut(s) 480
Mox20I TGGCCA 1 cut(s) 173
Mph1103I ATGCAT 1 cut(s) 1346
MroXI GAANNNNTTC 1 cut(s) 1414
MscI TGGCCA 1 cut(s) 173
MseI TTAA 7 cut(s) 86, 396, 981, 1335, 1361, 1439, 1487
Msp20I TGGCCA 1 cut(s) 173
MspI CCGG 1 cut(s) 281
MspR9I CCNGG 4 cut(s) 269, 281, 994, 1047
MvaI CCWGG 3 cut(s) 269, 994, 1047
NciI CCSGG 1 cut(s) 281
NcoI CCATGG 1 cut(s) 1009
NdeI CATATG 1 cut(s) 1021
NdeII GATC 5 cut(s) 709, 717, 989, 1542, 1547
NlaIII CATG 8 cut(s) 643, 847, 877, 977, 1013, 1331, 1344, 1376
NlaIV GGNNCC 1 cut(s) 991
NsiI ATGCAT 1 cut(s) 1346
NspI RCATGY 1 cut(s) 643
PaeR7I CTCGAG 2 cut(s) 273, 852
PceI AGGCCT 1 cut(s) 1229
PciI ACATGT 1 cut(s) 639
PciSI GCTCTTC 1 cut(s) 1587
PdmI GAANNNNTTC 1 cut(s) 1414
PfeI GAWTC 4 cut(s) 107, 682, 1016, 1313
PkrI GCNGC 7 cut(s) 616, 673, 834, 949, 1264, 1432, 1477
Ple19I CGATCG 1 cut(s) 1550
Ppu21I YACGTR 1 cut(s) 728
PscI ACATGT 1 cut(s) 639
PshBI ATTAAT 1 cut(s) 1335
PsiI TTATAA 1 cut(s) 1274
Psp124BI GAGCTC 1 cut(s) 1582
Psp1406I AACGTT 1 cut(s) 132
Psp6I CCWGG 3 cut(s) 267, 992, 1045
PspEI GGTNACC 2 cut(s) 549, 1041
PspFI CCCAGC 4 cut(s) 95, 545, 1126, 1297
PspGI CCWGG 3 cut(s) 267, 992, 1045
PspN4I GGNNCC 1 cut(s) 991
PspPI GGNCC 1 cut(s) 265
PspXI VCTCGAGB 1 cut(s) 852
PstNI CAGNNNCTG 1 cut(s) 560
PsuI RGATCY 1 cut(s) 989
PvuI CGATCG 1 cut(s) 1550
RsaI GTAC 3 cut(s) 66, 888, 1592
RsaNI GTAC 3 cut(s) 65, 887, 1591
SacI GAGCTC 1 cut(s) 1582
SapI GCTCTTC 1 cut(s) 1587
SaqAI TTAA 7 cut(s) 86, 396, 981, 1335, 1361, 1439, 1487
SatI GCNGC 7 cut(s) 615, 672, 833, 948, 1263, 1431, 1476
Sau3AI GATC 5 cut(s) 709, 717, 989, 1542, 1547
Sau96I GGNCC 1 cut(s) 265
ScaI AGTACT 1 cut(s) 888
ScrFI CCNGG 4 cut(s) 269, 281, 994, 1047
SduI GDGCHC 1 cut(s) 1582
SexAI ACCWGGT 1 cut(s) 1045
SfaNI GCATC 8 cut(s) 80, 325, 837, 875, 934, 1483, 1487, 1510
Sfr274I CTCGAG 2 cut(s) 273, 852
SinI GGWCC 1 cut(s) 265
SlaI CTCGAG 2 cut(s) 273, 852
SmlI CTYRAG 7 cut(s) 273, 443, 654, 686, 852, 1096, 1103
SmoI CTYRAG 7 cut(s) 273, 443, 654, 686, 852, 1096, 1103
Sse9I AATT 7 cut(s) 17, 982, 1090, 1145, 1163, 1217, 1336
SseBI AGGCCT 1 cut(s) 1229
SsiI CCGC 3 cut(s) 410, 892, 1431
SspMI CTAG 3 cut(s) 381, 1235, 1517
SstI GAGCTC 1 cut(s) 1582
StuI AGGCCT 1 cut(s) 1229
StyD4I CCNGG 4 cut(s) 267, 279, 992, 1045
StyI CCWWGG 4 cut(s) 380, 1009, 1251, 1320
TaaI ACNGT 2 cut(s) 571, 1184
TaiI ACGT 2 cut(s) 135, 730
TaqI TCGA 7 cut(s) 118, 242, 274, 566, 720, 853, 1550
TasI AATT 7 cut(s) 17, 982, 1090, 1145, 1163, 1217, 1336
TatI WGTACW 3 cut(s) 64, 886, 1590
TauI GCSGC 1 cut(s) 1433
TfiI GAWTC 4 cut(s) 107, 682, 1016, 1313
Tru1I TTAA 7 cut(s) 86, 396, 981, 1335, 1361, 1439, 1487
Tru9I TTAA 7 cut(s) 86, 396, 981, 1335, 1361, 1439, 1487
TscAI CASTG 4 cut(s) 443, 868, 1312, 1545
TseI GCWGC 6 cut(s) 614, 671, 832, 947, 1262, 1475
TspDTI ATGAA 6 cut(s) 225, 773, 1316, 1329, 1401, 1574
TspGWI ACGGA 1 cut(s) 1411
TspRI CASTG 4 cut(s) 443, 868, 1312, 1545
VpaK11BI GGWCC 1 cut(s) 265
VspI ATTAAT 1 cut(s) 1335
XapI RAATTY 2 cut(s) 17, 1090
XbaI TCTAGA 1 cut(s) 1234
XceI RCATGY 1 cut(s) 643
XhoI CTCGAG 2 cut(s) 273, 852
XmaJI CCTAGG 1 cut(s) 380
XmiI GTMKAC 1 cut(s) 1425
XmnI GAANNNNTTC 1 cut(s) 1414
XspI CTAG 3 cut(s) 381, 1235, 1517
ZrmI AGTACT 1 cut(s) 888
Zsp2I ATGCAT 1 cut(s) 1346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.