AT1G02270

calcium-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0013430
Physical Location & Seq
Reverse (-)
443118 .. 446296
3179 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G02270.1

Sequence Viewer

Length: 1455 bp
ATGCAAAGCCATCAGAATCGAAGCAGGCTGAGCTTGATTCTACGGTCTAGATTCATCCCTTCCTTCTCTTCACCACTCTCCCTCTTCGTCGTCCTCGCTGCTGTTCCTCTTCCGATCTACTTTTCCGGTCTACTCTCCGGCAGAAACAACAAGCCGCTTTCAGTTATGAGACTCAACTCCAACTTAGCTTCTTCAATGGTGGAATCGAATATTTCTTGTACCACTTTCAATATCTTGGCTCCGATTTATAAACGCGTTGATCAAAAGAATCATAGCACTCGAGAGAGCGATTTCCGCACGCTTTGGTTAGCTCGTAACCAGAGGATTTTAGATTTGTTACTCCATCAACGCTCTTCAGTTATCTGTCTCCAGGAAGTTTGGGTTGGGAATGAGGAATTAGTGAACATGTACCACCACCAGCTTTCATCTTCAGGCTACACTATTTATCAGTTGGCTAGAACAAACAGTCGTGGTGATGGTCTTTTAACAGCCATTCATAAGGACCATTTTAAAGTTGTAAATTACCGGGAGCTGCTCTTTAACGATTTTGGAGACCGTGTTGCTCAGCTCTTACATGTCAAAACAGTTATTCCCTTTCCGCTTAATGGAAAGCAAGACGTTCAACAAGAAGTTATTATAGTGAACACTCACCTCTTGTTTCCTCACGATTCTAGTTTGTCTATTGTAAGACTGCATCAGGTTTACAAAATTCTTGAATATCTGGAAGCTTACCAAAAGGAAAATAAACTTAATCACATGCCCATTATTCTCTGCGGTGACTGGAATGGAAGCAAACGCGGGCATGTCTACAAATTTTTGAGATCTCAAGGGTTTATTTCATCATACGATGATGCTCATCAGTATACCGACAGTGATGCTCATAGGTGGGTTAGCCACAGAAACCACAGGGGAAATATTTGCGGAGTTGATTTCATATGGCTCTGTAACCCTAGTGATTCAAGAAAACCATTGAGAACAAGTTGGGTGGAAGCTGTTTTCAGCATTATCAAGTATCAACTACACAAAGCTTCCATAGCCGAGGACGATGCCTTTACTTTTCTTGGGGCAAAAAATCACAGTGATTCACTAACCTACTCTGACTTTTGCCTAGCACTTCAAAAGGTAAATCTAACGGGTATTCCACATGGACTTAGCTTCGAAGAGACAAAAGAGCTATGGGTCCGAGCTGATCTTGATGGAAATGGTGTCTTCGACTATGAAGAACTTAAGAAAATTTGGAACATGACGATGGTAAATCAACCTGGAAACTGCAAAGAGAGTGTGATGGAGAGTAAGAAAGAAGAAGGAGAAGATGAAGCAATCGGATTGAAAGTGAATAAAGCAATTTTGTTTCCACAAGAAGCAGAGAAAGGATTGTGGCCGGAGAATTACAATATATCCGATCATGCTTGTCTCACCGTACAATTCTCTCCAGTCAAAATGCTTTGTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_8 Exo_endo_phos

Protein Analysis

484

Amino Acids

55.47

Weight (kDa)

7.68

Isoelectric Point (pI)

47.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 101 - 316 4.7e-10 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013430)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02270 AT1G02270
fragaria_vesca FvH4_7g18080 FvH4_7g18080 FvH4_7g18080
malus_domestica MD01G1095200.v1.1 MD07G1163500.v1.1 MD07G1164200.v1.1
prunus_persica Prupe.2G203200_v2.0.a1
pyrus_communis pycom01g11740 pycom07g15930
rosa_chinensis RchiOBHm_Chr1g0361711
rosa_laevigata RLG00000027708
rosa_multiflora Rmu_sc0003426.1_g000004
rosa_rugosa Rorug01G0291000
rosa_samantha Rh1BG264200 Rh1DG294200
rosa_wichuraiana Rw1G026710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 249
AccI GTMKAC 3 cut(s) 130, 807, 863
AccII CGCG 2 cut(s) 255, 798
AciI CCGC 6 cut(s) 155, 295, 599, 774, 798, 921
AcoI YGGCCR 1 cut(s) 1379
AcsI RAATTY 3 cut(s) 708, 812, 1233
AcuI CTGAAG 2 cut(s) 339, 414
AfaI GTAC 3 cut(s) 220, 410, 1422
AfiI CCNNNNNNNGG 1 cut(s) 605
AflII CTTAAG 1 cut(s) 1226
AflIII ACRYGT 3 cut(s) 253, 405, 574
AgsI TTSAA 7 cut(s) 195, 229, 623, 716, 960, 1118, 1330
AjnI CCWGG 2 cut(s) 369, 1261
AjuI GAANNNNNNNTTGG 2 cut(s) 366, 398
Alw26I GTCTC 5 cut(s) 163, 371, 546, 1157, 1418
Ama87I CYCGRG 1 cut(s) 279
AoxI GGCC 1 cut(s) 1379
ApeKI GCWGC 2 cut(s) 98, 532
ApoI RAATTY 3 cut(s) 708, 812, 1233
AspS9I GGNCC 2 cut(s) 502, 1180
AsuC2I CCSGG 1 cut(s) 527
AsuHPI GGTGA 5 cut(s) 63, 485, 641, 788, 1408
AsuII TTCGAA 1 cut(s) 1158
AvaI CYCGRG 1 cut(s) 279
AvaII GGWCC 2 cut(s) 502, 1180
BbsI GAAGAC 1 cut(s) 1201
BbvI GCAGC 2 cut(s) 85, 519
BccI CCATC 6 cut(s) 18, 351, 470, 1190, 1243, 1279
BcgI CGANNNNNNTGC 4 cut(s) 857, 891, 1028, 1062
BciT130I CCWGG 2 cut(s) 371, 1263
BclI TGATCA 1 cut(s) 259
BcnI CCSGG 1 cut(s) 527
BcoDI GTCTC 5 cut(s) 163, 371, 546, 1157, 1418
BfaI CTAG 5 cut(s) 48, 456, 672, 951, 1109
BfrI CTTAAG 1 cut(s) 1226
BglII AGATCT 1 cut(s) 821
BisI GCNGC 3 cut(s) 99, 155, 533
BlpI GCTNAGC 2 cut(s) 29, 564
BlsI GCNGC 3 cut(s) 100, 156, 534
Bme1390I CCNGG 3 cut(s) 371, 527, 1263
Bme18I GGWCC 2 cut(s) 502, 1180
BmeT110I CYCGRG 1 cut(s) 279
BmgT120I GGNCC 2 cut(s) 502, 1180
BmiI GGNNCC 2 cut(s) 240, 1181
BmrFI CCNGG 3 cut(s) 371, 527, 1263
BmsI GCATC 4 cut(s) 703, 841, 865, 1036
BpiI GAAGAC 1 cut(s) 1201
BpmI CTGGAG 2 cut(s) 353, 1416
Bpu1102I GCTNAGC 2 cut(s) 29, 564
Bpu14I TTCGAA 1 cut(s) 1158
BpuEI CTTGAG 1 cut(s) 810
BpuMI CCSGG 1 cut(s) 527
BsaBI GATNNNNATC 1 cut(s) 855
BsaI GGTCTC 1 cut(s) 546
BsaJI CCNNGG 1 cut(s) 1038
BsaWI WCCGGW 1 cut(s) 125
BsaXI ACNNNNNCTCC 2 cut(s) 543, 573
Bsc4I CCNNNNNNNGG 1 cut(s) 605
Bse1I ACTGG 2 cut(s) 785, 1433
Bse8I GATNNNNATC 1 cut(s) 855
BseBI CCWGG 2 cut(s) 371, 1263
BseDI CCNNGG 1 cut(s) 1038
BseGI GGATG 1 cut(s) 54
BseJI GATNNNNATC 1 cut(s) 855
BseLI CCNNNNNNNGG 1 cut(s) 605
BseMII CTCAG 2 cut(s) 20, 578
BseNI ACTGG 2 cut(s) 785, 1433
BseXI GCAGC 2 cut(s) 85, 519
Bsh1236I CGCG 2 cut(s) 255, 798
BshFI GGCC 1 cut(s) 1381
BsiHKCI CYCGRG 1 cut(s) 279
BsiSI CCGG 4 cut(s) 126, 138, 526, 1382
BslI CCNNNNNNNGG 1 cut(s) 605
BsmAI GTCTC 5 cut(s) 163, 371, 546, 1157, 1418
BsnI GGCC 1 cut(s) 1381
Bso31I GGTCTC 1 cut(s) 546
BsoBI CYCGRG 1 cut(s) 279
Bsp119I TTCGAA 1 cut(s) 1158
Bsp143I GATC 5 cut(s) 114, 259, 821, 1189, 1402
Bsp1720I GCTNAGC 2 cut(s) 29, 564
BspACI CCGC 6 cut(s) 155, 295, 599, 774, 798, 921
BspANI GGCC 1 cut(s) 1381
BspCNI CTCAG 2 cut(s) 21, 577
BspFNI CGCG 2 cut(s) 255, 798
BspLI GGNNCC 2 cut(s) 240, 1181
BspQI GCTCTTC 1 cut(s) 358
BspT104I TTCGAA 1 cut(s) 1158
BspTI CTTAAG 1 cut(s) 1226
BspTNI GGTCTC 1 cut(s) 546
BsrI ACTGG 2 cut(s) 785, 1433
BssECI CCNNGG 1 cut(s) 1038
BssMI GATC 5 cut(s) 114, 259, 821, 1189, 1402
BssNAI GTATAC 1 cut(s) 864
Bst1107I GTATAC 1 cut(s) 864
Bst2UI CCWGG 2 cut(s) 371, 1263
Bst4CI ACNGT 7 cut(s) 45, 467, 557, 586, 872, 1079, 1420
Bst6I CTCTTC 5 cut(s) 73, 89, 114, 358, 1155
BstAFI CTTAAG 1 cut(s) 1226
BstBI TTCGAA 1 cut(s) 1158
BstC8I GCNNGC 3 cut(s) 26, 299, 800
BstDEI CTNAG 4 cut(s) 29, 184, 564, 1151
BstF5I GGATG 1 cut(s) 54
BstFNI CGCG 2 cut(s) 255, 798
BstKTI GATC 5 cut(s) 117, 262, 824, 1192, 1405
BstMAI GTCTC 5 cut(s) 163, 371, 546, 1157, 1418
BstMBI GATC 5 cut(s) 114, 259, 821, 1189, 1402
BstMWI GCNNNNNNNGC 3 cut(s) 30, 294, 1034
BstNI CCWGG 2 cut(s) 371, 1263
BstNSI RCATGY 4 cut(s) 409, 578, 760, 806
BstSCI CCNGG 3 cut(s) 369, 525, 1261
BstUI CGCG 2 cut(s) 255, 798
BstV1I GCAGC 2 cut(s) 85, 519
BstV2I GAAGAC 1 cut(s) 1201
BstX2I RGATCY 1 cut(s) 821
BstYI RGATCY 1 cut(s) 821
BstZ17I GTATAC 1 cut(s) 864
BsuRI GGCC 1 cut(s) 1381
BtsCI GGATG 1 cut(s) 54
BtsIMutI CAGTG 2 cut(s) 877, 1084
Cac8I GCNNGC 3 cut(s) 26, 299, 800
Cfr13I GGNCC 2 cut(s) 502, 1180
Csp6I GTAC 3 cut(s) 219, 409, 1421
CspCI CAANNNNNGTGG 2 cut(s) 966, 1001
CviAII CATG 7 cut(s) 406, 575, 757, 803, 1145, 1243, 1406
CviQI GTAC 3 cut(s) 219, 409, 1421
DdeI CTNAG 4 cut(s) 29, 184, 564, 1151
DpnI GATC 5 cut(s) 116, 261, 823, 1191, 1404
DpnII GATC 5 cut(s) 114, 259, 821, 1189, 1402
DraI TTTAAA 1 cut(s) 511
EaeI YGGCCR 1 cut(s) 1379
Eam1104I CTCTTC 5 cut(s) 73, 89, 114, 358, 1155
EarI CTCTTC 5 cut(s) 73, 89, 114, 358, 1155
Eco31I GGTCTC 1 cut(s) 546
Eco47I GGWCC 2 cut(s) 502, 1180
Eco57I CTGAAG 2 cut(s) 339, 414
Eco88I CYCGRG 1 cut(s) 279
EcoRII CCWGG 2 cut(s) 369, 1261
FaeI CATG 7 cut(s) 409, 578, 760, 806, 1148, 1246, 1409
FatI CATG 7 cut(s) 405, 574, 756, 802, 1144, 1242, 1405
FauI CCCGC 1 cut(s) 791
FauNDI CATATG 1 cut(s) 935
FbaI TGATCA 1 cut(s) 259
FblI GTMKAC 3 cut(s) 130, 807, 863
Fnu4HI GCNGC 3 cut(s) 99, 155, 533
FokI GGATG 1 cut(s) 41
Fsp4HI GCNGC 3 cut(s) 99, 155, 533
FspBI CTAG 5 cut(s) 48, 456, 672, 951, 1109
GluI GCNGC 3 cut(s) 99, 155, 533
GsuI CTGGAG 2 cut(s) 353, 1416
HaeIII GGCC 1 cut(s) 1381
HapII CCGG 4 cut(s) 126, 138, 526, 1382
Hin1II CATG 7 cut(s) 409, 578, 760, 806, 1148, 1246, 1409
HindIII AAGCTT 2 cut(s) 726, 1026
HinfI GANTC 9 cut(s) 16, 37, 51, 171, 203, 268, 668, 956, 1082
HpaII CCGG 4 cut(s) 126, 138, 526, 1382
HphI GGTGA 5 cut(s) 63, 485, 641, 788, 1408
Hpy166II GTNNAC 6 cut(s) 131, 403, 643, 703, 808, 864
Hpy188I TCNGA 7 cut(s) 15, 114, 243, 1099, 1184, 1325, 1402
Hpy188III TCNNGA 7 cut(s) 48, 281, 665, 713, 722, 960, 1193
Hpy8I GTNNAC 6 cut(s) 131, 403, 643, 703, 808, 864
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 3 cut(s) 69, 73, 1298
HpyCH4III ACNGT 7 cut(s) 45, 467, 557, 586, 872, 1079, 1420
HpyCH4IV ACGT 1 cut(s) 618
HpyCH4V TGCA 3 cut(s) 4, 694, 1272
HpyF10VI GCNNNNNNNGC 3 cut(s) 30, 294, 1034
HpyF3I CTNAG 4 cut(s) 29, 184, 564, 1151
HpySE526I ACGT 1 cut(s) 618
Hsp92II CATG 7 cut(s) 409, 578, 760, 806, 1148, 1246, 1409
Ksp22I TGATCA 1 cut(s) 259
Kzo9I GATC 5 cut(s) 114, 259, 821, 1189, 1402
LguI GCTCTTC 1 cut(s) 358
LmnI GCTCC 2 cut(s) 244, 529
Lsp1109I GCAGC 2 cut(s) 85, 519
LweI GCATC 4 cut(s) 703, 841, 865, 1036
MaeI CTAG 5 cut(s) 48, 456, 672, 951, 1109
MaeII ACGT 1 cut(s) 618
MaeIII GTNAC 4 cut(s) 314, 336, 776, 944
MalI GATC 5 cut(s) 116, 261, 823, 1191, 1404
MboI GATC 5 cut(s) 114, 259, 821, 1189, 1402
MflI RGATCY 1 cut(s) 821
MluCI AATT 8 cut(s) 395, 520, 708, 812, 1233, 1344, 1387, 1424
MluI ACGCGT 1 cut(s) 253
MlyI GAGTC 1 cut(s) 165
MmeI TCCRAC 1 cut(s) 204
MnlI CCTC 8 cut(s) 92, 104, 117, 315, 385, 662, 672, 1033
MseI TTAA 6 cut(s) 485, 510, 540, 603, 750, 1227
MslI CAYNNNNRTG 1 cut(s) 1247
MspCI CTTAAG 1 cut(s) 1226
MspI CCGG 4 cut(s) 126, 138, 526, 1382
MspR9I CCNGG 3 cut(s) 371, 527, 1263
MvaI CCWGG 2 cut(s) 371, 1263
MvnI CGCG 2 cut(s) 255, 798
MwoI GCNNNNNNNGC 3 cut(s) 30, 294, 1034
NciI CCSGG 1 cut(s) 527
NdeI CATATG 1 cut(s) 935
NdeII GATC 5 cut(s) 114, 259, 821, 1189, 1402
NlaIII CATG 7 cut(s) 409, 578, 760, 806, 1148, 1246, 1409
NlaIV GGNNCC 2 cut(s) 240, 1181
NmeAIII GCCGAG 1 cut(s) 1063
NmuCI GTSAC 1 cut(s) 776
NspI RCATGY 4 cut(s) 409, 578, 760, 806
NspV TTCGAA 1 cut(s) 1158
PaeR7I CTCGAG 1 cut(s) 279
PciI ACATGT 2 cut(s) 405, 574
PciSI GCTCTTC 1 cut(s) 358
PfeI GAWTC 8 cut(s) 16, 37, 51, 203, 268, 668, 956, 1082
PfoI TCCNGGA 1 cut(s) 369
PkrI GCNGC 3 cut(s) 100, 156, 534
PleI GAGTC 1 cut(s) 165
PpsI GAGTC 1 cut(s) 165
PscI ACATGT 2 cut(s) 405, 574
PsiI TTATAA 1 cut(s) 249
Psp6I CCWGG 2 cut(s) 369, 1261
PspGI CCWGG 2 cut(s) 369, 1261
PspN4I GGNNCC 2 cut(s) 240, 1181
PspPI GGNCC 2 cut(s) 502, 1180
PsuI RGATCY 1 cut(s) 821
RsaI GTAC 3 cut(s) 220, 410, 1422
RsaNI GTAC 3 cut(s) 219, 409, 1421
RseI CAYNNNNRTG 1 cut(s) 1247
SapI GCTCTTC 1 cut(s) 358
SaqAI TTAA 6 cut(s) 485, 510, 540, 603, 750, 1227
SatI GCNGC 3 cut(s) 99, 155, 533
Sau3AI GATC 5 cut(s) 114, 259, 821, 1189, 1402
Sau96I GGNCC 2 cut(s) 502, 1180
SchI GAGTC 1 cut(s) 165
ScrFI CCNGG 3 cut(s) 371, 527, 1263
SfaNI GCATC 4 cut(s) 703, 841, 865, 1036
Sfr274I CTCGAG 1 cut(s) 279
SfuI TTCGAA 1 cut(s) 1158
SinI GGWCC 2 cut(s) 502, 1180
SlaI CTCGAG 1 cut(s) 279
SmiMI CAYNNNNRTG 1 cut(s) 1247
SmlI CTYRAG 3 cut(s) 279, 825, 1226
SmoI CTYRAG 3 cut(s) 279, 825, 1226
Sse9I AATT 8 cut(s) 395, 520, 708, 812, 1233, 1344, 1387, 1424
SsiI CCGC 6 cut(s) 155, 295, 599, 774, 798, 921
SspI AATATT 2 cut(s) 211, 916
SspMI CTAG 5 cut(s) 48, 456, 672, 951, 1109
StyD4I CCNGG 3 cut(s) 369, 525, 1261
TaaI ACNGT 7 cut(s) 45, 467, 557, 586, 872, 1079, 1420
TaiI ACGT 1 cut(s) 621
TaqI TCGA 5 cut(s) 19, 206, 280, 1158, 1212
TasI AATT 8 cut(s) 395, 520, 708, 812, 1233, 1344, 1387, 1424
TauI GCSGC 1 cut(s) 157
TfiI GAWTC 8 cut(s) 16, 37, 51, 203, 268, 668, 956, 1082
Tru1I TTAA 6 cut(s) 485, 510, 540, 603, 750, 1227
Tru9I TTAA 6 cut(s) 485, 510, 540, 603, 750, 1227
TscAI CASTG 2 cut(s) 877, 1084
TseFI GTSAC 1 cut(s) 776
TseI GCWGC 2 cut(s) 98, 532
Tsp45I GTSAC 1 cut(s) 776
TspDTI ATGAA 7 cut(s) 43, 414, 485, 828, 922, 1233, 1329
TspRI CASTG 2 cut(s) 877, 1084
Vha464I CTTAAG 1 cut(s) 1226
VpaK11BI GGWCC 2 cut(s) 502, 1180
XapI RAATTY 3 cut(s) 708, 812, 1233
XbaI TCTAGA 1 cut(s) 47
XceI RCATGY 4 cut(s) 409, 578, 760, 806
XhoI CTCGAG 1 cut(s) 279
XmiI GTMKAC 3 cut(s) 130, 807, 863
XspI CTAG 5 cut(s) 48, 456, 672, 951, 1109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.