FvH4_7g18080

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0013430
Physical Location & Seq
Reverse (-)
15198579 .. 15202852
4274 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g18080.t1

Sequence Viewer

Length: 1356 bp
ATGGTCGCAAACCGTAATATGAGAGACAAAGACTCCAATCCTAATGTCCATGAGAGACAAAGCTTTAATGGAAGCTTTGTATCAATGGAGGAGCCAAAGCCAGAGACAAGGGCAGGGCCTGGTTTGATTTCTTGTACTACTTTCAATATTCTGGCGCCAATCTACAAACGAATCGATCAACAGAATCAAGGGCTTCGTGAGAGTCAATTCAGCTCATTCTGGGTGACTAGGAATCAGAAGATTTTGGATTGGTTACTGTATGAATCGTCTAGTATTGTATGTCTCCAGGAGTTCTGGGTTGGAAATGAAGAATTTGTGAATATGTACCTGAAGAGGCTTGGTGATGCTGGCTATAGTACCTTCAAGCTTGCCCGAACTAACAACCGTGGAGATGGTCTTCTGACTGCTGTTCGAAGTGATTGCTTTAGAGTTCTGAATTATCGAGAGTTGCACTTCAATGATTTTGGAGACCGTGTTGCCCAGCTATTACATGTTCAATTAACTGCTCCCTTCTCACAAATCCAGAGAGGCAACCTTCAACAAGAAATTCTTATTGTGAACACTCACTTGTTATTCCCTCATGATTCCAGTCTTTGTATTGTTTACAAAATTTTGCAATTTGTGGAGGCTTATCAAAATGAAAACAAGCTAAACCATATGCCCATCATACTCTGTGGTGACTGGAATGGAAGCAAGCGTGGACATGTATACAAATTCCTTAGATCACAGGGGTTTGAATCAACATATGACACTGCTCATCAATATACCGACAATGACGCCCACAAGTGGGTTAGCCACCGCAACCATAGAGGAAACATATGTGGAGTGGACTTCATTTGGCTTTGTAATCCTAACAAGTCCCGTAAACCATTGAAGACTAGTTGGTGTGAAGCTGTTTTCGGAATATTAAGGCGCCAGCTTCGAAAAGCTTCAATGGCTGAAAATGATGCATTTGCCTTTTTGAAGGGCGACAACCATGTTGATTTTATTACTTCCTTAGCTTTTTGTGAAGCACTCCGTCAGGTTAATTTGATTGGTCATCCATCCGGACTGGGTTTCCAGGAGACCAGGGATCTCTGGATCCAAGCAGACACAGATGCAAATGGTGTCCTTGATTATGAAGAATTCAAGAATATGATCTGGAGCTGTACAGGGTCAGACCAAAAGGAAAGCCTCACTGGGAGTAGAAAGGAGTCTGAGGAAGACAGTAAGGAAGCGCTTGGATTCAAGGTGAAGAACGCTGTTCTTTACCCAAGTGAGGCAGAGAAGGGAATATGGCCTGAAAACTATACACTGTCGGATCATGCTAGATTGACCGTTGTACTTTCACCAGAAAACATGTGGTGTTGTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_8 Exo_endo_phos

Protein Analysis

452

Amino Acids

51.84

Weight (kDa)

6.41

Isoelectric Point (pI)

41.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 74 - 297 2.8e-09 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013430)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02270 AT1G02270
fragaria_vesca FvH4_7g18080 FvH4_7g18080 FvH4_7g18080
malus_domestica MD01G1095200.v1.1 MD07G1163500.v1.1 MD07G1164200.v1.1
prunus_persica Prupe.2G203200_v2.0.a1
pyrus_communis pycom01g11740 pycom07g15930
rosa_chinensis RchiOBHm_Chr1g0361711
rosa_laevigata RLG00000027708
rosa_multiflora Rmu_sc0003426.1_g000004
rosa_rugosa Rorug01G0291000
rosa_samantha Rh1BG264200 Rh1DG294200
rosa_wichuraiana Rw1G026710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 154, 912
AccI GTMKAC 1 cut(s) 708
AccIII TCCGGA 1 cut(s) 1046
AciI CCGC 1 cut(s) 799
AclWI GGATC 4 cut(s) 1075, 1080, 1088, 1308
AcsI RAATTY 5 cut(s) 311, 546, 609, 713, 1124
AcuI CTGAAG 1 cut(s) 350
AcyI GRCGYC 3 cut(s) 155, 777, 913
AfaI GTAC 5 cut(s) 136, 326, 358, 1150, 1323
AfeI AGCGCT 1 cut(s) 1218
AfiI CCNNNNNNNGG 3 cut(s) 786, 787, 1258
AflIII ACRYGT 3 cut(s) 490, 703, 1338
AhlI ACTAGT 1 cut(s) 878
AjnI CCWGG 4 cut(s) 118, 285, 1059, 1067
Alw26I GTCTC 6 cut(s) 18, 49, 98, 287, 462, 1058
AlwI GGATC 4 cut(s) 1075, 1080, 1088, 1308
AlwNI CAGNNNCTG 1 cut(s) 119
Aor13HI TCCGGA 1 cut(s) 1046
Aor51HI AGCGCT 1 cut(s) 1218
AoxI GGCC 2 cut(s) 116, 1277
ApoI RAATTY 5 cut(s) 311, 546, 609, 713, 1124
Asp700I GAANNNNTTC 1 cut(s) 928
AspLEI GCGC 3 cut(s) 157, 915, 1219
AspS9I GGNCC 1 cut(s) 116
AsuHPI GGTGA 5 cut(s) 235, 353, 689, 1243, 1320
AsuII TTCGAA 2 cut(s) 412, 922
BamHI GGATCC 1 cut(s) 1080
BanI GGYRCC 2 cut(s) 154, 912
BbsI GAAGAC 3 cut(s) 389, 881, 1209
BccI CCATC 3 cut(s) 386, 671, 1051
BcgI CGANNNNNNTGC 2 cut(s) 402, 436
BciT130I CCWGG 4 cut(s) 120, 287, 1061, 1069
BcoDI GTCTC 6 cut(s) 18, 49, 98, 287, 462, 1058
BcuI ACTAGT 1 cut(s) 878
BfaI CTAG 4 cut(s) 228, 270, 879, 1308
BfmI CTRYAG 1 cut(s) 352
BfoI RGCGCY 3 cut(s) 158, 916, 1220
Bme1390I CCNGG 4 cut(s) 120, 287, 1061, 1069
BmgT120I GGNCC 1 cut(s) 116
BmiI GGNNCC 4 cut(s) 93, 156, 914, 1082
BmrFI CCNGG 4 cut(s) 120, 287, 1061, 1069
BmrI ACTGGG 2 cut(s) 1061, 1188
BmsI GCATC 3 cut(s) 334, 937, 1087
BmuI ACTGGG 2 cut(s) 1061, 1188
BpiI GAAGAC 3 cut(s) 389, 881, 1209
BpmI CTGGAG 2 cut(s) 269, 1162
Bpu10I CCTNAGC 1 cut(s) 997
Bpu14I TTCGAA 2 cut(s) 412, 922
Bsa29I ATCGAT 1 cut(s) 174
BsaHI GRCGYC 3 cut(s) 155, 777, 913
BsaI GGTCTC 2 cut(s) 462, 1058
BsaJI CCNNGG 2 cut(s) 385, 1068
BsaWI WCCGGW 1 cut(s) 1046
BsaXI ACNNNNNCTCC 4 cut(s) 17, 47, 459, 489
Bsc4I CCNNNNNNNGG 3 cut(s) 786, 787, 1258
Bse1I ACTGG 4 cut(s) 588, 686, 1056, 1183
BseAI TCCGGA 1 cut(s) 1046
BseBI CCWGG 4 cut(s) 120, 287, 1061, 1069
BseCI ATCGAT 1 cut(s) 174
BseDI CCNNGG 2 cut(s) 385, 1068
BseGI GGATG 2 cut(s) 1039, 1043
BseLI CCNNNNNNNGG 3 cut(s) 786, 787, 1258
BseMII CTCAG 1 cut(s) 1188
BseNI ACTGG 4 cut(s) 588, 686, 1056, 1183
BseRI GAGGAG 1 cut(s) 104
BseYI CCCAGC 1 cut(s) 480
BshFI GGCC 2 cut(s) 118, 1279
BshNI GGYRCC 2 cut(s) 154, 912
BshVI ATCGAT 1 cut(s) 174
BsiSI CCGG 1 cut(s) 1047
BslFI GGGAC 1 cut(s) 844
BslI CCNNNNNNNGG 3 cut(s) 786, 787, 1258
BsmAI GTCTC 6 cut(s) 18, 49, 98, 287, 462, 1058
BsmFI GGGAC 1 cut(s) 844
BsnI GGCC 2 cut(s) 118, 1279
Bso31I GGTCTC 2 cut(s) 462, 1058
Bsp119I TTCGAA 2 cut(s) 412, 922
Bsp13I TCCGGA 1 cut(s) 1046
Bsp1407I TGTACA 1 cut(s) 1148
Bsp143I GATC 6 cut(s) 175, 722, 1072, 1080, 1137, 1300
BspACI CCGC 1 cut(s) 799
BspANI GGCC 2 cut(s) 118, 1279
BspCNI CTCAG 1 cut(s) 1189
BspDI ATCGAT 1 cut(s) 174
BspEI TCCGGA 1 cut(s) 1046
BspHI TCATGA 1 cut(s) 580
BspLI GGNNCC 4 cut(s) 93, 156, 914, 1082
BspPI GGATC 4 cut(s) 1075, 1080, 1088, 1308
BspT104I TTCGAA 2 cut(s) 412, 922
BspT107I GGYRCC 2 cut(s) 154, 912
BspTNI GGTCTC 2 cut(s) 462, 1058
BsrGI TGTACA 1 cut(s) 1148
BsrI ACTGG 4 cut(s) 588, 686, 1056, 1183
BssECI CCNNGG 2 cut(s) 385, 1068
BssMI GATC 6 cut(s) 175, 722, 1072, 1080, 1137, 1300
BssNAI GTATAC 1 cut(s) 709
BssNI GRCGYC 3 cut(s) 155, 777, 913
Bst1107I GTATAC 1 cut(s) 709
Bst2UI CCWGG 4 cut(s) 120, 287, 1061, 1069
Bst4CI ACNGT 7 cut(s) 14, 258, 386, 473, 1208, 1296, 1318
Bst6I CTCTTC 1 cut(s) 326
BstACI GRCGYC 3 cut(s) 155, 777, 913
BstAUI TGTACA 1 cut(s) 1148
BstBI TTCGAA 2 cut(s) 412, 922
BstC8I GCNNGC 4 cut(s) 349, 369, 695, 917
BstDEI CTNAG 4 cut(s) 719, 997, 1197, 1353
BstDSI CCRYGG 1 cut(s) 385
BstF5I GGATG 2 cut(s) 1039, 1043
BstH2I RGCGCY 3 cut(s) 158, 916, 1220
BstHHI GCGC 3 cut(s) 157, 915, 1219
BstKTI GATC 6 cut(s) 178, 725, 1075, 1083, 1140, 1303
BstMAI GTCTC 6 cut(s) 18, 49, 98, 287, 462, 1058
BstMBI GATC 6 cut(s) 175, 722, 1072, 1080, 1137, 1300
BstMWI GCNNNNNNNGC 1 cut(s) 935
BstNI CCWGG 4 cut(s) 120, 287, 1061, 1069
BstNSI RCATGY 3 cut(s) 494, 707, 1342
BstSCI CCNGG 4 cut(s) 118, 285, 1059, 1067
BstSFI CTRYAG 1 cut(s) 352
BstV2I GAAGAC 3 cut(s) 389, 881, 1209
BstX2I RGATCY 2 cut(s) 1072, 1080
BstYI RGATCY 2 cut(s) 1072, 1080
BstZ17I GTATAC 1 cut(s) 709
Bsu15I ATCGAT 1 cut(s) 174
BsuRI GGCC 2 cut(s) 118, 1279
BsuTUI ATCGAT 1 cut(s) 174
BtgI CCRYGG 1 cut(s) 385
BtsCI GGATG 2 cut(s) 1039, 1043
BtsI GCAGTG 1 cut(s) 750
BtsIMutI CAGTG 3 cut(s) 750, 1176, 1292
Cac8I GCNNGC 4 cut(s) 349, 369, 695, 917
CaiI CAGNNNCTG 1 cut(s) 119
CciI TCATGA 1 cut(s) 580
CfoI GCGC 3 cut(s) 157, 915, 1219
Cfr13I GGNCC 1 cut(s) 116
ClaI ATCGAT 1 cut(s) 174
CseI GACGC 1 cut(s) 785
Csp6I GTAC 5 cut(s) 135, 325, 357, 1149, 1322
CviAII CATG 7 cut(s) 50, 491, 581, 704, 977, 1304, 1339
CviQI GTAC 5 cut(s) 135, 325, 357, 1149, 1322
DdeI CTNAG 4 cut(s) 719, 997, 1197, 1353
DinI GGCGCC 2 cut(s) 156, 914
DpnI GATC 6 cut(s) 177, 724, 1074, 1082, 1139, 1302
DpnII GATC 6 cut(s) 175, 722, 1072, 1080, 1137, 1300
Eam1104I CTCTTC 1 cut(s) 326
EarI CTCTTC 1 cut(s) 326
Eco31I GGTCTC 2 cut(s) 462, 1058
Eco47III AGCGCT 1 cut(s) 1218
Eco57I CTGAAG 1 cut(s) 350
EcoO109I RGGNCCY 1 cut(s) 116
EcoRI GAATTC 1 cut(s) 1124
EcoRII CCWGG 4 cut(s) 118, 285, 1059, 1067
EcoT22I ATGCAT 1 cut(s) 952
EgeI GGCGCC 2 cut(s) 156, 914
EheI GGCGCC 2 cut(s) 156, 914
FaeI CATG 7 cut(s) 53, 494, 584, 707, 980, 1307, 1342
FalI AAGNNNNNCTT 2 cut(s) 534, 566
FaqI GGGAC 1 cut(s) 844
FatI CATG 7 cut(s) 49, 490, 580, 703, 976, 1303, 1338
FauNDI CATATG 3 cut(s) 657, 745, 818
FblI GTMKAC 1 cut(s) 708
FokI GGATG 2 cut(s) 1026, 1030
FspBI CTAG 4 cut(s) 228, 270, 879, 1308
GlaI GCGC 3 cut(s) 156, 914, 1218
GsaI CCCAGC 1 cut(s) 484
GsuI CTGGAG 2 cut(s) 269, 1162
HaeII RGCGCY 3 cut(s) 158, 916, 1220
HaeIII GGCC 2 cut(s) 118, 1279
HapII CCGG 1 cut(s) 1047
HgaI GACGC 1 cut(s) 785
HhaI GCGC 3 cut(s) 157, 915, 1219
Hin1I GRCGYC 3 cut(s) 155, 777, 913
Hin1II CATG 7 cut(s) 53, 494, 584, 707, 980, 1307, 1342
Hin6I GCGC 3 cut(s) 155, 913, 1217
HinP1I GCGC 3 cut(s) 155, 913, 1217
HindIII AAGCTT 4 cut(s) 61, 73, 365, 927
HpaII CCGG 1 cut(s) 1047
HphI GGTGA 5 cut(s) 235, 353, 689, 1243, 1320
Hpy166II GTNNAC 6 cut(s) 559, 604, 701, 709, 829, 866
Hpy188I TCNGA 7 cut(s) 237, 402, 435, 902, 1159, 1198, 1300
Hpy188III TCNNGA 8 cut(s) 197, 443, 523, 581, 1047, 1078, 1129, 1141
Hpy8I GTNNAC 6 cut(s) 559, 604, 701, 709, 829, 866
HpyAV CCTTC 5 cut(s) 370, 520, 545, 958, 1261
HpyCH4III ACNGT 7 cut(s) 14, 258, 386, 473, 1208, 1296, 1318
HpyCH4V TGCA 4 cut(s) 451, 616, 950, 1100
HpyF10VI GCNNNNNNNGC 1 cut(s) 935
HpyF3I CTNAG 4 cut(s) 719, 997, 1197, 1353
Hsp92I GRCGYC 3 cut(s) 155, 777, 913
Hsp92II CATG 7 cut(s) 53, 494, 584, 707, 980, 1307, 1342
HspAI GCGC 3 cut(s) 155, 913, 1217
KasI GGCGCC 2 cut(s) 154, 912
Kpn2I TCCGGA 1 cut(s) 1046
Kzo9I GATC 6 cut(s) 175, 722, 1072, 1080, 1137, 1300
LmnI GCTCC 3 cut(s) 91, 511, 1143
LweI GCATC 3 cut(s) 334, 937, 1087
MaeI CTAG 4 cut(s) 228, 270, 879, 1308
MaeIII GTNAC 3 cut(s) 223, 252, 677
MalI GATC 6 cut(s) 177, 724, 1074, 1082, 1139, 1302
MboI GATC 6 cut(s) 175, 722, 1072, 1080, 1137, 1300
MboII GAAGA 8 cut(s) 250, 320, 343, 389, 886, 1133, 1214, 1246
MflI RGATCY 2 cut(s) 1072, 1080
Mly113I GGCGCC 2 cut(s) 155, 913
MlyI GAGTC 3 cut(s) 26, 211, 1202
MmeI TCCRAC 2 cut(s) 280, 1278
MnlI CCTC 9 cut(s) 82, 327, 521, 588, 619, 803, 1184, 1192, 1252
Mph1103I ATGCAT 1 cut(s) 952
MroI TCCGGA 1 cut(s) 1046
MroXI GAANNNNTTC 1 cut(s) 928
MseI TTAA 4 cut(s) 66, 500, 908, 1026
MslI CAYNNNNRTG 1 cut(s) 456
MspI CCGG 1 cut(s) 1047
MspR9I CCNGG 4 cut(s) 120, 287, 1061, 1069
MvaI CCWGG 4 cut(s) 120, 287, 1061, 1069
MwoI GCNNNNNNNGC 1 cut(s) 935
NarI GGCGCC 2 cut(s) 155, 913
NdeI CATATG 3 cut(s) 657, 745, 818
NdeII GATC 6 cut(s) 175, 722, 1072, 1080, 1137, 1300
NlaIII CATG 7 cut(s) 53, 494, 584, 707, 980, 1307, 1342
NlaIV GGNNCC 4 cut(s) 93, 156, 914, 1082
NmuCI GTSAC 2 cut(s) 223, 677
NsiI ATGCAT 1 cut(s) 952
NspI RCATGY 3 cut(s) 494, 707, 1342
NspV TTCGAA 2 cut(s) 412, 922
PagI TCATGA 1 cut(s) 580
PciI ACATGT 3 cut(s) 490, 703, 1338
PdmI GAANNNNTTC 1 cut(s) 928
PfeI GAWTC 7 cut(s) 171, 184, 232, 263, 584, 737, 1224
PfoI TCCNGGA 2 cut(s) 285, 1059
PleI GAGTC 3 cut(s) 26, 210, 1201
PluTI GGCGCC 2 cut(s) 158, 916
PpsI GAGTC 3 cut(s) 26, 210, 1201
PscI ACATGT 3 cut(s) 490, 703, 1338
Psp6I CCWGG 4 cut(s) 118, 285, 1059, 1067
PspFI CCCAGC 1 cut(s) 480
PspGI CCWGG 4 cut(s) 118, 285, 1059, 1067
PspN4I GGNNCC 4 cut(s) 93, 156, 914, 1082
PspPI GGNCC 1 cut(s) 116
PstNI CAGNNNCTG 1 cut(s) 119
PsuI RGATCY 2 cut(s) 1072, 1080
RsaI GTAC 5 cut(s) 136, 326, 358, 1150, 1323
RsaNI GTAC 5 cut(s) 135, 325, 357, 1149, 1322
RseI CAYNNNNRTG 1 cut(s) 456
SaqAI TTAA 4 cut(s) 66, 500, 908, 1026
Sau3AI GATC 6 cut(s) 175, 722, 1072, 1080, 1137, 1300
Sau96I GGNCC 1 cut(s) 116
SchI GAGTC 3 cut(s) 26, 211, 1202
ScrFI CCNGG 4 cut(s) 120, 287, 1061, 1069
SfaNI GCATC 3 cut(s) 334, 937, 1087
SfcI CTRYAG 1 cut(s) 352
SfoI GGCGCC 2 cut(s) 156, 914
SfuI TTCGAA 2 cut(s) 412, 922
SmiMI CAYNNNNRTG 1 cut(s) 456
SpeI ACTAGT 1 cut(s) 878
SsiI CCGC 1 cut(s) 799
SspDI GGCGCC 2 cut(s) 154, 912
SspI AATATT 2 cut(s) 148, 906
SspMI CTAG 4 cut(s) 228, 270, 879, 1308
StyD4I CCNGG 4 cut(s) 118, 285, 1059, 1067
TaaI ACNGT 7 cut(s) 14, 258, 386, 473, 1208, 1296, 1318
TaqI TCGA 4 cut(s) 174, 412, 442, 922
TatI WGTACW 3 cut(s) 134, 1148, 1321
TfiI GAWTC 7 cut(s) 171, 184, 232, 263, 584, 737, 1224
Tru1I TTAA 4 cut(s) 66, 500, 908, 1026
Tru9I TTAA 4 cut(s) 66, 500, 908, 1026
TscAI CASTG 3 cut(s) 757, 1183, 1299
TseFI GTSAC 2 cut(s) 223, 677
Tsp45I GTSAC 2 cut(s) 223, 677
TspDTI ATGAA 5 cut(s) 276, 321, 654, 823, 1134
TspGWI ACGGA 1 cut(s) 1007
TspRI CASTG 3 cut(s) 757, 1183, 1299
XapI RAATTY 5 cut(s) 311, 546, 609, 713, 1124
XceI RCATGY 3 cut(s) 494, 707, 1342
XcmI CCANNNNNNNNNTGG 1 cut(s) 1338
XmiI GTMKAC 1 cut(s) 708
XmnI GAANNNNTTC 1 cut(s) 928
XspI CTAG 4 cut(s) 228, 270, 879, 1308
Zsp2I ATGCAT 1 cut(s) 952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.