MD01G1095200.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0013430
Physical Location & Seq
Reverse (-)
21025812 .. 21029584
3773 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1095200.v1.1.491

Sequence Viewer

Length: 1389 bp
ATGGGTTTAGGTGGAAGTTCTAATGTGGCAAAGATCAATCGCAGAGAAAGTGAGAGCCTTTCAAGGAGCTATAGCGGCAGCTGCACTTCGTTTCCGTCGATATCAGAGCCAAAGCCAGAGACTTTGGCAGGGCCTTGCTTAGTTTCGTGCACGACTTTCAACATTTTGGCTCCAATCTATAAACGACTCGATCAACAGAATCAAAGCCATCGAGAGAGCGACTACAGGGCGTTTTGGGTGGCCAGGAACCAGAGGATTTTGGATAGGTTGCTCTATGAATCATCTGCCATTATTTGTCTGCAGGAGTTCTGGGTTGGAAATGAAGAATTTGTGAATATGTACCTGGACAGGCTTGGTGATGCAGGCTATACGACCTTCAAGCTTGCCCGAACTAACAACCGGGGTGACGGTTTGCTGACTGCTGTGCGGAGGGATTGCTTTAGTGTTCTAAACTATCGAGAGTTGCATTTTAATGATTTTGGAGATCGTGTTGCTCAGCTGTTACATGTTCAGTTAGCTTCCCCATTTTCACAAAACCAGAGAGGAAACGTTCAGCAAGAAATGCTAATTGTGAATACTCACTTGTTATTTCCTCACGATTCCAGTCTCTCTTTAGTGCGATTGTATCAGGTTTACAAAATTTTGCAATTTGTGGAGTCATATCAGAATGAAAATAAGCTAAACCCGATGCCCATCGTACTCTGTGGTGACTGGAACGGAAGTAAGCGTGGCCATGTGTACAAATTCCTTAGATCGCAGGGGTTTGAATCAACATATGATACTGCTCATCAATATACTGATGCGGATGCTCACAAGTGGGTTAGCCACCGTAATCATAGGGGAAACATCTGCGGTGTTGACTTCATTTGGCTTTGTAATCCCAATAAGTCACGAAAACCACTAAAGACTAGTTGGTGCGAAGCTGTTTTTGGAATATTACAGCGCCAGCTCCGAAAAGCTTCGATGGCTGAAAATGATGCATTTGCCTTTCTAAAGGGTGATAGTCATGGTGATTTTATTACGACCACAGCTTTCTGTGAAGGACTACATCAGGTTAACTTAATTGGTCATCCTACGGGACTAGGTTTCCAGGAGACGAGGGATCTCTGGATCCAAGCAGATGCCAACGCAAATGGTGTCCTTGATTACGAAGAATTTAAGAATAGAATCTGGAGTTCGACAGCATCAGAGGAAAAGGAAAGCCTCATCGGTAGTAGAGAGGAGTCCAAACGAGGCACGCAGGGCGCCCTCGGATTCAATGTGAAGAATGCAGTTCTATATCCCCGTGAGGCGGAGAAGGGAATATGGCCCGATGATTACACTCTCTCTGACCATGCTCGACTATCGGTTGTACTTTCACCAGAAAGGATGTGGTGTTGCCAGTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_8 Exo_endo_phos

Protein Analysis

463

Amino Acids

52.63

Weight (kDa)

6.92

Isoelectric Point (pI)

40.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 52 - 307 1.6e-09 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013430)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02270 AT1G02270
fragaria_vesca FvH4_7g18080 FvH4_7g18080 FvH4_7g18080
malus_domestica MD01G1095200.v1.1 MD07G1163500.v1.1 MD07G1164200.v1.1
prunus_persica Prupe.2G203200_v2.0.a1
pyrus_communis pycom01g11740 pycom07g15930
rosa_chinensis RchiOBHm_Chr1g0361711
rosa_laevigata RLG00000027708
rosa_multiflora Rmu_sc0003426.1_g000004
rosa_rugosa Rorug01G0291000
rosa_samantha Rh1BG264200 Rh1DG294200
rosa_wichuraiana Rw1G026710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1244
AciI CCGC 5 cut(s) 75, 427, 803, 852, 1292
AclI AACGTT 1 cut(s) 549
AclWI GGATC 3 cut(s) 1105, 1110, 1118
AcoI YGGCCR 2 cut(s) 240, 730
AcsI RAATTY 4 cut(s) 326, 639, 743, 1154
AcyI GRCGYC 1 cut(s) 1245
AfaI GTAC 4 cut(s) 341, 699, 740, 1353
AfiI CCNNNNNNNGG 1 cut(s) 1291
AflIII ACRYGT 1 cut(s) 505
AgsI TTSAA 5 cut(s) 63, 160, 379, 767, 1258
AhlI ACTAGT 1 cut(s) 908
AjnI CCWGG 3 cut(s) 242, 342, 1089
AjuI GAANNNNNNNTTGG 2 cut(s) 912, 944
Alw21I GWGCWC 1 cut(s) 152
Alw26I GTCTC 3 cut(s) 113, 611, 1088
Alw44I GTGCAC 1 cut(s) 148
AlwI GGATC 3 cut(s) 1105, 1110, 1118
AoxI GGCC 4 cut(s) 131, 240, 730, 1307
ApaLI GTGCAC 1 cut(s) 148
ApeKI GCWGC 2 cut(s) 78, 81
ApoI RAATTY 4 cut(s) 326, 639, 743, 1154
Asp700I GAANNNNTTC 1 cut(s) 958
AspLEI GCGC 2 cut(s) 945, 1247
AspS9I GGNCC 2 cut(s) 131, 1308
AsuC2I CCSGG 1 cut(s) 401
AsuHPI GGTGA 6 cut(s) 368, 416, 719, 1010, 1022, 1350
BaeGI GKGCMC 1 cut(s) 152
BalI TGGCCA 2 cut(s) 242, 732
BamHI GGATCC 1 cut(s) 1110
BanI GGYRCC 1 cut(s) 1244
Bbv12I GWGCWC 1 cut(s) 152
BbvI GCAGC 2 cut(s) 68, 90
BccI CCATC 3 cut(s) 216, 701, 958
BciT130I CCWGG 3 cut(s) 244, 344, 1091
BcnI CCSGG 1 cut(s) 401
BcoDI GTCTC 3 cut(s) 113, 611, 1088
BcuI ACTAGT 1 cut(s) 908
BfaI CTAG 2 cut(s) 909, 1082
BfmI CTRYAG 3 cut(s) 70, 223, 299
BfoI RGCGCY 2 cut(s) 946, 1248
BisI GCNGC 3 cut(s) 76, 79, 82
BlpI GCTNAGC 1 cut(s) 495
BlsI GCNGC 3 cut(s) 77, 80, 83
Bme1390I CCNGG 4 cut(s) 244, 344, 401, 1091
BmgT120I GGNCC 2 cut(s) 131, 1308
BmiI GGNNCC 4 cut(s) 171, 248, 1112, 1246
BmrFI CCNGG 4 cut(s) 244, 344, 401, 1091
BmsI GCATC 7 cut(s) 349, 678, 790, 796, 967, 1111, 1193
BpmI CTGGAG 1 cut(s) 1192
Bpu1102I GCTNAGC 1 cut(s) 495
BpuMI CCSGG 1 cut(s) 401
BsaBI GATNNNNATC 1 cut(s) 692
BsaHI GRCGYC 1 cut(s) 1245
BsaJI CCNNGG 2 cut(s) 400, 1249
BsaXI ACNNNNNCTCC 2 cut(s) 474, 504
Bsc4I CCNNNNNNNGG 1 cut(s) 1291
Bse1I ACTGG 3 cut(s) 603, 716, 1381
Bse8I GATNNNNATC 1 cut(s) 692
BseBI CCWGG 3 cut(s) 244, 344, 1091
BseDI CCNNGG 2 cut(s) 400, 1249
BseGI GGATG 3 cut(s) 811, 1069, 1374
BseJI GATNNNNATC 1 cut(s) 692
BseLI CCNNNNNNNGG 1 cut(s) 1291
BseMII CTCAG 1 cut(s) 509
BseNI ACTGG 3 cut(s) 603, 716, 1381
BseRI GAGGAG 1 cut(s) 1235
BseSI GKGCMC 1 cut(s) 152
BseXI GCAGC 2 cut(s) 68, 90
BsgI GTGCAG 1 cut(s) 67
BshFI GGCC 4 cut(s) 133, 242, 732, 1309
BshNI GGYRCC 1 cut(s) 1244
BsiHKAI GWGCWC 1 cut(s) 152
BsiSI CCGG 1 cut(s) 400
BslFI GGGAC 1 cut(s) 1092
BslI CCNNNNNNNGG 1 cut(s) 1291
BsmAI GTCTC 3 cut(s) 113, 611, 1088
BsmBI CGTCTC 1 cut(s) 1088
BsmFI GGGAC 1 cut(s) 1092
BsmI GAATGC 1 cut(s) 1273
BsnI GGCC 4 cut(s) 133, 242, 732, 1309
Bsp1286I GDGCHC 1 cut(s) 152
Bsp1407I TGTACA 1 cut(s) 738
Bsp143I GATC 6 cut(s) 33, 190, 484, 752, 1102, 1110
Bsp1720I GCTNAGC 1 cut(s) 495
BspACI CCGC 5 cut(s) 75, 427, 803, 852, 1292
BspANI GGCC 4 cut(s) 133, 242, 732, 1309
BspCNI CTCAG 1 cut(s) 508
BspLI GGNNCC 4 cut(s) 171, 248, 1112, 1246
BspMAI CTGCAG 1 cut(s) 303
BspPI GGATC 3 cut(s) 1105, 1110, 1118
BspT107I GGYRCC 1 cut(s) 1244
BsrGI TGTACA 1 cut(s) 738
BsrI ACTGG 3 cut(s) 603, 716, 1381
BssECI CCNNGG 2 cut(s) 400, 1249
BssMI GATC 6 cut(s) 33, 190, 484, 752, 1102, 1110
BssNI GRCGYC 1 cut(s) 1245
Bst2UI CCWGG 3 cut(s) 244, 344, 1091
Bst4CI ACNGT 2 cut(s) 410, 830
BstACI GRCGYC 1 cut(s) 1245
BstAPI GCANNNNNTGC 1 cut(s) 562
BstAUI TGTACA 1 cut(s) 738
BstC8I GCNNGC 4 cut(s) 364, 384, 947, 1238
BstDEI CTNAG 3 cut(s) 139, 495, 749
BstF5I GGATG 3 cut(s) 811, 1069, 1374
BstH2I RGCGCY 2 cut(s) 946, 1248
BstHHI GCGC 2 cut(s) 945, 1247
BstKTI GATC 6 cut(s) 36, 193, 487, 755, 1105, 1113
BstMAI GTCTC 3 cut(s) 113, 611, 1088
BstMBI GATC 6 cut(s) 33, 190, 484, 752, 1102, 1110
BstMWI GCNNNNNNNGC 5 cut(s) 75, 81, 562, 965, 1242
BstNI CCWGG 3 cut(s) 244, 344, 1091
BstNSI RCATGY 1 cut(s) 509
BstSCI CCNGG 4 cut(s) 242, 342, 399, 1089
BstSFI CTRYAG 3 cut(s) 70, 223, 299
BstSLI GKGCMC 1 cut(s) 152
BstV1I GCAGC 2 cut(s) 68, 90
BstX2I RGATCY 2 cut(s) 1102, 1110
BstYI RGATCY 2 cut(s) 1102, 1110
BsuRI GGCC 4 cut(s) 133, 242, 732, 1309
BtsCI GGATG 3 cut(s) 811, 1069, 1374
Cac8I GCNNGC 4 cut(s) 364, 384, 947, 1238
CfoI GCGC 2 cut(s) 945, 1247
Cfr13I GGNCC 2 cut(s) 131, 1308
Csp6I GTAC 4 cut(s) 340, 698, 739, 1352
CviAII CATG 4 cut(s) 506, 734, 1007, 1334
CviQI GTAC 4 cut(s) 340, 698, 739, 1352
DdeI CTNAG 3 cut(s) 139, 495, 749
DinI GGCGCC 1 cut(s) 1246
DpnI GATC 6 cut(s) 35, 192, 486, 754, 1104, 1112
DpnII GATC 6 cut(s) 33, 190, 484, 752, 1102, 1110
EaeI YGGCCR 2 cut(s) 240, 730
EciI GGCGGA 1 cut(s) 1307
Eco32I GATATC 1 cut(s) 102
EcoO109I RGGNCCY 1 cut(s) 131
EcoRII CCWGG 3 cut(s) 242, 342, 1089
EcoRV GATATC 1 cut(s) 102
EcoT22I ATGCAT 1 cut(s) 982
EgeI GGCGCC 1 cut(s) 1246
EheI GGCGCC 1 cut(s) 1246
Esp3I CGTCTC 1 cut(s) 1088
FaeI CATG 4 cut(s) 509, 737, 1010, 1337
FaqI GGGAC 1 cut(s) 1092
FatI CATG 4 cut(s) 505, 733, 1006, 1333
FauNDI CATATG 1 cut(s) 775
Fnu4HI GCNGC 3 cut(s) 76, 79, 82
FokI GGATG 3 cut(s) 818, 1056, 1381
Fsp4HI GCNGC 3 cut(s) 76, 79, 82
FspBI CTAG 2 cut(s) 909, 1082
GlaI GCGC 2 cut(s) 944, 1246
GluI GCNGC 3 cut(s) 76, 79, 82
GsuI CTGGAG 1 cut(s) 1192
HaeII RGCGCY 2 cut(s) 946, 1248
HaeIII GGCC 4 cut(s) 133, 242, 732, 1309
HapII CCGG 1 cut(s) 400
HhaI GCGC 2 cut(s) 945, 1247
Hin1I GRCGYC 1 cut(s) 1245
Hin1II CATG 4 cut(s) 509, 737, 1010, 1337
Hin6I GCGC 2 cut(s) 943, 1245
HinP1I GCGC 2 cut(s) 943, 1245
HincII GTYRAC 2 cut(s) 859, 1057
HindII GTYRAC 2 cut(s) 859, 1057
HindIII AAGCTT 2 cut(s) 380, 957
HinfI GANTC 9 cut(s) 186, 199, 278, 599, 656, 767, 1167, 1223, 1254
HpaI GTTAAC 1 cut(s) 1057
HpaII CCGG 1 cut(s) 400
HphI GGTGA 6 cut(s) 368, 416, 719, 1010, 1022, 1350
Hpy166II GTNNAC 5 cut(s) 150, 634, 739, 859, 1057
Hpy188I TCNGA 6 cut(s) 106, 666, 953, 1189, 1253, 1330
Hpy188III TCNNGA 6 cut(s) 212, 458, 596, 891, 1108, 1171
Hpy8I GTNNAC 5 cut(s) 150, 634, 739, 859, 1057
Hpy99I CGWCG 1 cut(s) 100
HpyAV CCTTC 3 cut(s) 385, 1034, 1291
HpyCH4III ACNGT 2 cut(s) 410, 830
HpyCH4IV ACGT 1 cut(s) 549
HpyCH4V TGCA 8 cut(s) 84, 150, 301, 362, 466, 646, 980, 1271
HpyF10VI GCNNNNNNNGC 5 cut(s) 75, 81, 562, 965, 1242
HpyF3I CTNAG 3 cut(s) 139, 495, 749
HpySE526I ACGT 1 cut(s) 549
Hsp92I GRCGYC 1 cut(s) 1245
Hsp92II CATG 4 cut(s) 509, 737, 1010, 1337
HspAI GCGC 2 cut(s) 943, 1245
KasI GGCGCC 1 cut(s) 1244
KspAI GTTAAC 1 cut(s) 1057
Kzo9I GATC 6 cut(s) 33, 190, 484, 752, 1102, 1110
LmnI GCTCC 3 cut(s) 66, 175, 954
Lsp1109I GCAGC 2 cut(s) 68, 90
LweI GCATC 7 cut(s) 349, 678, 790, 796, 967, 1111, 1193
MaeI CTAG 2 cut(s) 909, 1082
MaeII ACGT 1 cut(s) 549
MaeIII GTNAC 4 cut(s) 404, 501, 707, 888
MalI GATC 6 cut(s) 35, 192, 486, 754, 1104, 1112
MboI GATC 6 cut(s) 33, 190, 484, 752, 1102, 1110
MboII GAAGA 3 cut(s) 335, 1163, 1276
MflI RGATCY 2 cut(s) 1102, 1110
MhlI GDGCHC 1 cut(s) 152
MlsI TGGCCA 2 cut(s) 242, 732
MluCI AATT 7 cut(s) 326, 567, 639, 647, 743, 1062, 1154
MluNI TGGCCA 2 cut(s) 242, 732
Mly113I GGCGCC 1 cut(s) 1245
MlyI GAGTC 3 cut(s) 180, 665, 1232
MmeI TCCRAC 1 cut(s) 295
Mox20I TGGCCA 2 cut(s) 242, 732
Mph1103I ATGCAT 1 cut(s) 982
MroXI GAANNNNTTC 1 cut(s) 958
MscI TGGCCA 2 cut(s) 242, 732
MseI TTAA 4 cut(s) 471, 1056, 1061, 1158
MslI CAYNNNNRTG 1 cut(s) 471
Msp20I TGGCCA 2 cut(s) 242, 732
MspA1I CMGCKG 2 cut(s) 81, 499
MspI CCGG 1 cut(s) 400
MspR9I CCNGG 4 cut(s) 244, 344, 401, 1091
Mva1269I GAATGC 1 cut(s) 1273
MvaI CCWGG 3 cut(s) 244, 344, 1091
MwoI GCNNNNNNNGC 5 cut(s) 75, 81, 562, 965, 1242
NarI GGCGCC 1 cut(s) 1245
NciI CCSGG 1 cut(s) 401
NdeI CATATG 1 cut(s) 775
NdeII GATC 6 cut(s) 33, 190, 484, 752, 1102, 1110
NlaIII CATG 4 cut(s) 509, 737, 1010, 1337
NlaIV GGNNCC 4 cut(s) 171, 248, 1112, 1246
NmuCI GTSAC 3 cut(s) 404, 707, 888
NsiI ATGCAT 1 cut(s) 982
NspI RCATGY 1 cut(s) 509
PciI ACATGT 1 cut(s) 505
PcsI WCGNNNNNNNCGW 1 cut(s) 95
PctI GAATGC 1 cut(s) 1273
PdmI GAANNNNTTC 1 cut(s) 958
PfeI GAWTC 6 cut(s) 199, 278, 599, 767, 1167, 1254
PfoI TCCNGGA 1 cut(s) 1089
PkrI GCNGC 3 cut(s) 77, 80, 83
PleI GAGTC 3 cut(s) 180, 664, 1231
PluTI GGCGCC 1 cut(s) 1248
PpsI GAGTC 3 cut(s) 180, 664, 1231
PscI ACATGT 1 cut(s) 505
Psp1406I AACGTT 1 cut(s) 549
Psp6I CCWGG 3 cut(s) 242, 342, 1089
PspGI CCWGG 3 cut(s) 242, 342, 1089
PspN4I GGNNCC 4 cut(s) 171, 248, 1112, 1246
PspPI GGNCC 2 cut(s) 131, 1308
PstI CTGCAG 1 cut(s) 303
PsuI RGATCY 2 cut(s) 1102, 1110
PvuII CAGCTG 2 cut(s) 81, 499
RsaI GTAC 4 cut(s) 341, 699, 740, 1353
RsaNI GTAC 4 cut(s) 340, 698, 739, 1352
RseI CAYNNNNRTG 1 cut(s) 471
SaqAI TTAA 4 cut(s) 471, 1056, 1061, 1158
SatI GCNGC 3 cut(s) 76, 79, 82
Sau3AI GATC 6 cut(s) 33, 190, 484, 752, 1102, 1110
Sau96I GGNCC 2 cut(s) 131, 1308
SchI GAGTC 3 cut(s) 180, 665, 1232
ScrFI CCNGG 4 cut(s) 244, 344, 401, 1091
SduI GDGCHC 1 cut(s) 152
SfaNI GCATC 7 cut(s) 349, 678, 790, 796, 967, 1111, 1193
SfcI CTRYAG 3 cut(s) 70, 223, 299
SfoI GGCGCC 1 cut(s) 1246
SmiMI CAYNNNNRTG 1 cut(s) 471
SpeI ACTAGT 1 cut(s) 908
Sse9I AATT 7 cut(s) 326, 567, 639, 647, 743, 1062, 1154
SsiI CCGC 5 cut(s) 75, 427, 803, 852, 1292
SspDI GGCGCC 1 cut(s) 1244
SspI AATATT 1 cut(s) 936
SspMI CTAG 2 cut(s) 909, 1082
StyD4I CCNGG 4 cut(s) 242, 342, 399, 1089
TaaI ACNGT 2 cut(s) 410, 830
TaiI ACGT 1 cut(s) 552
TaqI TCGA 7 cut(s) 98, 189, 211, 457, 962, 1178, 1339
TasI AATT 7 cut(s) 326, 567, 639, 647, 743, 1062, 1154
TatI WGTACW 2 cut(s) 738, 1351
TauI GCSGC 1 cut(s) 78
TfiI GAWTC 6 cut(s) 199, 278, 599, 767, 1167, 1254
Tru1I TTAA 4 cut(s) 471, 1056, 1061, 1158
Tru9I TTAA 4 cut(s) 471, 1056, 1061, 1158
TseFI GTSAC 3 cut(s) 404, 707, 888
TseI GCWGC 2 cut(s) 78, 81
Tsp45I GTSAC 3 cut(s) 404, 707, 888
TspDTI ATGAA 4 cut(s) 291, 336, 684, 853
TspGWI ACGGA 2 cut(s) 84, 732
VneI GTGCAC 1 cut(s) 148
XapI RAATTY 4 cut(s) 326, 639, 743, 1154
XceI RCATGY 1 cut(s) 509
XcmI CCANNNNNNNNNTGG 1 cut(s) 1368
XmnI GAANNNNTTC 1 cut(s) 958
XspI CTAG 2 cut(s) 909, 1082
Zsp2I ATGCAT 1 cut(s) 982
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.