MD07G1164200.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0013430
Physical Location & Seq
Reverse (-)
23889576 .. 23893449
3874 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1164200.v1.1.491

Sequence Viewer

Length: 1389 bp
ATGGGTTTAGGGGCGGGTTCTAATGTGGCAAAGATTAATCGCAGAGAAAGTGAGAGTCTTTCAAGGAGCTACAGCGGCGGCTGCATTTCGTTGCCGTCGATAGCAGAGCCAAAGCCAGAGACTTTGGCAGGGCCTTGCTTAGTCTCGTGCACGACTTTCAATATTTTGGCTCCAATCTACAAAAGACTCGATCAACAGAATCAAAGCCTTAGAGAGAGCGACTACGGGGCGTTTTGGGTGGCCAGGAACCAGAGGATTTTGGATTGGTTGCTCTGTGAATCATCTGCCATTATTTGTCTGCAGGAGTTCTGGGTTGGAAATGAAGAATTTGTGAATATGTACCTGGACAGGCTTGGTGAAGCAGGCTATACTACCTTTAAGCTCGCCCGAACTAACAACCGGGGAGATGGTTTGCTGACTGCTGTGAGGAGGGACTGTTTTAGTGTTCGAAACTACCGAGAGTTGCATTTTAATGATTTTGGAGACCGTGTTGCTCAGCTATTACATGTTCAGTTAGCTACCCCATTTTCACAAAACCAGAGAGGAAACGTTCAACAAGAAATGCTAATTGTGAATACTCATTTGTTGTTTCCTCATGATTCCAGTCTCTCTATAGTACGATTGCATCAGGTTTACAAAATTTTGCAATTTGTGGAGTCATATCAGAATGAAAAGATGCTAAACCCGATGCCTATCGTACTCTGTGGTGACTGGAATGGAAGTAAGCAAGGCCATGTGTACAAATTCCTTAGATCACAGGGTTTTGAATCAACATATGATACTGCTCATCAATATACCGATGCGGATGCTCACAAGTGGGTTAGCCACCGCAATCATAGGGGAAACATCTGCGGTGTTGACTTCATTTGGCTTTGTAATCCCAATAAGTCACGAAAACCACTCAAGACTAGTTGGTGCGAAGCTGTTTTTGGAATATTACGGCGCCAGCTTCGAAAAGCTTCGATGGCTGAAAATGATGCATTTGCCTTTCTGAAGGGTGATAGTCATGGTGATTTTATTACGTCCTCAGCTTTCTGTGAAGCACTACAACAGGTTAATTTAATTGGTCATCCTGCGGGACTAGGTTTCCAGGAGACAAGGGATCTCTGGATCCAGGCAGACGCCGATGCAAATGGTGTTCTTGATTACGAAGAATTTAAGAATAGAATTTGGAGTTCAACAGCGTCAGAGGATAAGGAAAGTCTCAACGGTAGTAGAGAGGAGTCCAAACGAGGCATGCAGGACTCCCTGGGATTCAATGTGAAGAATGCAGTTCTATATCCCCGTGAGGTGGAGAAGGGAATATGGCCCGAAGATTATACTCTCTCCGATCACGCTCGACTCTCGGTTGTACTTTCACCAGAAAGGATGTGGTGTTGTAAGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_8 Exo_endo_phos

Protein Analysis

463

Amino Acids

52.54

Weight (kDa)

6.51

Isoelectric Point (pI)

41.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 52 - 307 2.5e-07 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013430)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02270 AT1G02270
fragaria_vesca FvH4_7g18080 FvH4_7g18080 FvH4_7g18080
malus_domestica MD01G1095200.v1.1 MD07G1163500.v1.1 MD07G1164200.v1.1
prunus_persica Prupe.2G203200_v2.0.a1
pyrus_communis pycom01g11740 pycom07g15930
rosa_chinensis RchiOBHm_Chr1g0361711
rosa_laevigata RLG00000027708
rosa_multiflora Rmu_sc0003426.1_g000004
rosa_rugosa Rorug01G0291000
rosa_samantha Rh1BG264200 Rh1DG294200
rosa_wichuraiana Rw1G026710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 942
AciI CCGC 7 cut(s) 14, 75, 78, 803, 829, 852, 1076
AclI AACGTT 1 cut(s) 549
AclWI GGATC 3 cut(s) 1105, 1110, 1118
AcoI YGGCCR 1 cut(s) 240
AcsI RAATTY 5 cut(s) 326, 639, 743, 1154, 1167
AcuI CTGAAG 1 cut(s) 1013
AcyI GRCGYC 2 cut(s) 943, 1122
AfaI GTAC 5 cut(s) 341, 618, 699, 740, 1353
AfiI CCNNNNNNNGG 1 cut(s) 1291
AflIII ACRYGT 1 cut(s) 505
AgsI TTSAA 6 cut(s) 63, 160, 554, 767, 1179, 1258
AhlI ACTAGT 1 cut(s) 908
AjnI CCWGG 5 cut(s) 242, 342, 1089, 1113, 1248
AjuI GAANNNNNNNTTGG 2 cut(s) 912, 944
AluBI AGCT 8 cut(s) 69, 382, 499, 518, 923, 949, 959, 1031
AluI AGCT 8 cut(s) 69, 382, 499, 518, 923, 949, 959, 1031
Alw21I GWGCWC 1 cut(s) 152
Alw26I GTCTC 6 cut(s) 113, 148, 477, 611, 1088, 1208
Alw44I GTGCAC 1 cut(s) 148
AlwI GGATC 3 cut(s) 1105, 1110, 1118
AoxI GGCC 4 cut(s) 131, 240, 730, 1307
ApaLI GTGCAC 1 cut(s) 148
ApeKI GCWGC 1 cut(s) 81
ApoI RAATTY 5 cut(s) 326, 639, 743, 1154, 1167
AseI ATTAAT 1 cut(s) 36
Asp700I GAANNNNTTC 1 cut(s) 958
AspLEI GCGC 1 cut(s) 945
AspS9I GGNCC 2 cut(s) 131, 1308
AsuC2I CCSGG 1 cut(s) 401
AsuHPI GGTGA 5 cut(s) 368, 719, 1010, 1022, 1350
AsuII TTCGAA 2 cut(s) 448, 952
BaeGI GKGCMC 1 cut(s) 152
BalI TGGCCA 1 cut(s) 242
BamHI GGATCC 1 cut(s) 1110
BanI GGYRCC 1 cut(s) 942
BauI CACGAG 1 cut(s) 145
Bbv12I GWGCWC 1 cut(s) 152
BbvCI CCTCAGC 1 cut(s) 1027
BbvI GCAGC 1 cut(s) 68
BccI CCATC 2 cut(s) 401, 958
BceAI ACGGC 2 cut(s) 79, 956
BcgI CGANNNNNNTGC 2 cut(s) 788, 822
BciT130I CCWGG 5 cut(s) 244, 344, 1091, 1115, 1250
BcnI CCSGG 1 cut(s) 401
BcoDI GTCTC 6 cut(s) 113, 148, 477, 611, 1088, 1208
BcuI ACTAGT 1 cut(s) 908
BfaI CTAG 2 cut(s) 909, 1082
BfmI CTRYAG 3 cut(s) 70, 299, 612
BfoI RGCGCY 1 cut(s) 946
BisI GCNGC 3 cut(s) 76, 79, 82
BlpI GCTNAGC 1 cut(s) 495
BlsI GCNGC 3 cut(s) 77, 80, 83
Bme1390I CCNGG 6 cut(s) 244, 344, 401, 1091, 1115, 1250
BmgT120I GGNCC 2 cut(s) 131, 1308
BmiI GGNNCC 4 cut(s) 171, 248, 944, 1112
BmrFI CCNGG 6 cut(s) 244, 344, 401, 1091, 1115, 1250
BmsI GCATC 7 cut(s) 634, 666, 678, 790, 796, 967, 1117
Bpu10I CCTNAGC 1 cut(s) 1027
Bpu1102I GCTNAGC 1 cut(s) 495
Bpu14I TTCGAA 2 cut(s) 448, 952
BpuEI CTTGAG 1 cut(s) 887
BpuMI CCSGG 1 cut(s) 401
BsaBI GATNNNNATC 1 cut(s) 692
BsaHI GRCGYC 2 cut(s) 943, 1122
BsaI GGTCTC 1 cut(s) 477
BsaJI CCNNGG 3 cut(s) 400, 1248, 1249
BsaXI ACNNNNNCTCC 4 cut(s) 421, 451, 474, 504
Bsc4I CCNNNNNNNGG 1 cut(s) 1291
Bse1I ACTGG 2 cut(s) 603, 716
Bse8I GATNNNNATC 1 cut(s) 692
BseBI CCWGG 5 cut(s) 244, 344, 1091, 1115, 1250
BseDI CCNNGG 3 cut(s) 400, 1248, 1249
BseGI GGATG 3 cut(s) 811, 1069, 1374
BseJI GATNNNNATC 1 cut(s) 692
BseLI CCNNNNNNNGG 1 cut(s) 1291
BseMII CTCAG 2 cut(s) 509, 1041
BseNI ACTGG 2 cut(s) 603, 716
BseRI GAGGAG 2 cut(s) 442, 1235
BseSI GKGCMC 1 cut(s) 152
BseXI GCAGC 1 cut(s) 68
BshFI GGCC 4 cut(s) 133, 242, 732, 1309
BshNI GGYRCC 1 cut(s) 942
BsiHKAI GWGCWC 1 cut(s) 152
BsiSI CCGG 1 cut(s) 400
BslFI GGGAC 2 cut(s) 446, 1092
BslI CCNNNNNNNGG 1 cut(s) 1291
BsmAI GTCTC 6 cut(s) 113, 148, 477, 611, 1088, 1208
BsmFI GGGAC 2 cut(s) 446, 1092
BsmI GAATGC 1 cut(s) 1273
BsnI GGCC 4 cut(s) 133, 242, 732, 1309
Bso31I GGTCTC 1 cut(s) 477
Bsp119I TTCGAA 2 cut(s) 448, 952
Bsp1286I GDGCHC 1 cut(s) 152
Bsp1407I TGTACA 1 cut(s) 738
Bsp143I GATC 5 cut(s) 190, 752, 1102, 1110, 1330
Bsp1720I GCTNAGC 1 cut(s) 495
BspACI CCGC 7 cut(s) 14, 75, 78, 803, 829, 852, 1076
BspANI GGCC 4 cut(s) 133, 242, 732, 1309
BspCNI CTCAG 2 cut(s) 508, 1040
BspHI TCATGA 1 cut(s) 595
BspLI GGNNCC 4 cut(s) 171, 248, 944, 1112
BspMAI CTGCAG 1 cut(s) 303
BspPI GGATC 3 cut(s) 1105, 1110, 1118
BspT104I TTCGAA 2 cut(s) 448, 952
BspT107I GGYRCC 1 cut(s) 942
BspTNI GGTCTC 1 cut(s) 477
BsrGI TGTACA 1 cut(s) 738
BsrI ACTGG 2 cut(s) 603, 716
BssECI CCNNGG 3 cut(s) 400, 1248, 1249
BssMI GATC 5 cut(s) 190, 752, 1102, 1110, 1330
BssNI GRCGYC 2 cut(s) 943, 1122
BssSI CACGAG 1 cut(s) 145
Bst2BI CACGAG 1 cut(s) 145
Bst2UI CCWGG 5 cut(s) 244, 344, 1091, 1115, 1250
Bst4CI ACNGT 3 cut(s) 437, 488, 1211
BstACI GRCGYC 2 cut(s) 943, 1122
BstAUI TGTACA 1 cut(s) 738
BstBI TTCGAA 2 cut(s) 448, 952
BstC8I GCNNGC 4 cut(s) 364, 384, 947, 1238
BstDEI CTNAG 5 cut(s) 139, 209, 495, 749, 1027
BstF5I GGATG 3 cut(s) 811, 1069, 1374
BstH2I RGCGCY 1 cut(s) 946
BstHHI GCGC 1 cut(s) 945
BstKTI GATC 5 cut(s) 193, 755, 1105, 1113, 1333
BstMAI GTCTC 6 cut(s) 113, 148, 477, 611, 1088, 1208
BstMBI GATC 5 cut(s) 190, 752, 1102, 1110, 1330
BstMWI GCNNNNNNNGC 3 cut(s) 75, 81, 965
BstNI CCWGG 5 cut(s) 244, 344, 1091, 1115, 1250
BstNSI RCATGY 2 cut(s) 509, 1240
BstSCI CCNGG 6 cut(s) 242, 342, 399, 1089, 1113, 1248
BstSFI CTRYAG 3 cut(s) 70, 299, 612
BstSLI GKGCMC 1 cut(s) 152
BstV1I GCAGC 1 cut(s) 68
BstX2I RGATCY 2 cut(s) 1102, 1110
BstYI RGATCY 2 cut(s) 1102, 1110
BsuRI GGCC 4 cut(s) 133, 242, 732, 1309
BtsCI GGATG 3 cut(s) 811, 1069, 1374
Cac8I GCNNGC 4 cut(s) 364, 384, 947, 1238
CciI TCATGA 1 cut(s) 595
CfoI GCGC 1 cut(s) 945
Cfr13I GGNCC 2 cut(s) 131, 1308
CseI GACGC 2 cut(s) 1130, 1173
Csp6I GTAC 5 cut(s) 340, 617, 698, 739, 1352
CviAII CATG 5 cut(s) 506, 596, 734, 1007, 1237
CviQI GTAC 5 cut(s) 340, 617, 698, 739, 1352
DdeI CTNAG 5 cut(s) 139, 209, 495, 749, 1027
DinI GGCGCC 1 cut(s) 944
DpnI GATC 5 cut(s) 192, 754, 1104, 1112, 1332
DpnII GATC 5 cut(s) 190, 752, 1102, 1110, 1330
EaeI YGGCCR 1 cut(s) 240
Eco31I GGTCTC 1 cut(s) 477
Eco57I CTGAAG 1 cut(s) 1013
EcoO109I RGGNCCY 1 cut(s) 131
EcoRII CCWGG 5 cut(s) 242, 342, 1089, 1113, 1248
EcoT22I ATGCAT 1 cut(s) 982
EgeI GGCGCC 1 cut(s) 944
EheI GGCGCC 1 cut(s) 944
FaeI CATG 5 cut(s) 509, 599, 737, 1010, 1240
FaqI GGGAC 2 cut(s) 446, 1092
FatI CATG 5 cut(s) 505, 595, 733, 1006, 1236
FauI CCCGC 2 cut(s) 7, 1069
FauNDI CATATG 1 cut(s) 775
Fnu4HI GCNGC 3 cut(s) 76, 79, 82
FokI GGATG 3 cut(s) 818, 1056, 1381
Fsp4HI GCNGC 3 cut(s) 76, 79, 82
FspBI CTAG 2 cut(s) 909, 1082
GlaI GCGC 1 cut(s) 944
GluI GCNGC 3 cut(s) 76, 79, 82
HaeII RGCGCY 1 cut(s) 946
HaeIII GGCC 4 cut(s) 133, 242, 732, 1309
HapII CCGG 1 cut(s) 400
HgaI GACGC 2 cut(s) 1130, 1173
HhaI GCGC 1 cut(s) 945
Hin1I GRCGYC 2 cut(s) 943, 1122
Hin1II CATG 5 cut(s) 509, 599, 737, 1010, 1240
Hin6I GCGC 1 cut(s) 943
HinP1I GCGC 1 cut(s) 943
HincII GTYRAC 1 cut(s) 859
HindII GTYRAC 1 cut(s) 859
HindIII AAGCTT 1 cut(s) 957
HpaII CCGG 1 cut(s) 400
HphI GGTGA 5 cut(s) 368, 719, 1010, 1022, 1350
Hpy166II GTNNAC 4 cut(s) 150, 634, 739, 859
Hpy188I TCNGA 4 cut(s) 666, 993, 1189, 1330
Hpy188III TCNNGA 5 cut(s) 596, 891, 904, 1108, 1142
Hpy8I GTNNAC 4 cut(s) 150, 634, 739, 859
Hpy99I CGWCG 1 cut(s) 100
HpyAV CCTTC 2 cut(s) 988, 1291
HpyCH4III ACNGT 3 cut(s) 437, 488, 1211
HpyCH4IV ACGT 2 cut(s) 549, 1022
HpyF10VI GCNNNNNNNGC 3 cut(s) 75, 81, 965
HpyF3I CTNAG 5 cut(s) 139, 209, 495, 749, 1027
HpySE526I ACGT 2 cut(s) 549, 1022
Hsp92I GRCGYC 2 cut(s) 943, 1122
Hsp92II CATG 5 cut(s) 509, 599, 737, 1010, 1240
HspAI GCGC 1 cut(s) 943
KasI GGCGCC 1 cut(s) 942
Kzo9I GATC 5 cut(s) 190, 752, 1102, 1110, 1330
LmnI GCTCC 2 cut(s) 66, 175
Lsp1109I GCAGC 1 cut(s) 68
LweI GCATC 7 cut(s) 634, 666, 678, 790, 796, 967, 1117
MaeI CTAG 2 cut(s) 909, 1082
MaeII ACGT 2 cut(s) 549, 1022
MaeIII GTNAC 2 cut(s) 707, 888
MalI GATC 5 cut(s) 192, 754, 1104, 1112, 1332
MboI GATC 5 cut(s) 190, 752, 1102, 1110, 1330
MboII GAAGA 4 cut(s) 335, 1163, 1276, 1325
MflI RGATCY 2 cut(s) 1102, 1110
MhlI GDGCHC 1 cut(s) 152
MlsI TGGCCA 1 cut(s) 242
MluCI AATT 9 cut(s) 326, 567, 639, 647, 743, 1057, 1062, 1154, 1167
MluNI TGGCCA 1 cut(s) 242
Mly113I GGCGCC 1 cut(s) 943
MlyI GAGTC 6 cut(s) 64, 180, 665, 1232, 1238, 1335
MmeI TCCRAC 1 cut(s) 295
Mox20I TGGCCA 1 cut(s) 242
Mph1103I ATGCAT 1 cut(s) 982
MroXI GAANNNNTTC 1 cut(s) 958
MscI TGGCCA 1 cut(s) 242
MseI TTAA 6 cut(s) 36, 378, 471, 1056, 1061, 1158
MslI CAYNNNNRTG 1 cut(s) 471
Msp20I TGGCCA 1 cut(s) 242
MspA1I CMGCKG 1 cut(s) 75
MspI CCGG 1 cut(s) 400
MspR9I CCNGG 6 cut(s) 244, 344, 401, 1091, 1115, 1250
Mva1269I GAATGC 1 cut(s) 1273
MvaI CCWGG 5 cut(s) 244, 344, 1091, 1115, 1250
MwoI GCNNNNNNNGC 3 cut(s) 75, 81, 965
NarI GGCGCC 1 cut(s) 943
NciI CCSGG 1 cut(s) 401
NdeI CATATG 1 cut(s) 775
NdeII GATC 5 cut(s) 190, 752, 1102, 1110, 1330
NlaIII CATG 5 cut(s) 509, 599, 737, 1010, 1240
NlaIV GGNNCC 4 cut(s) 171, 248, 944, 1112
NmuCI GTSAC 2 cut(s) 707, 888
NsiI ATGCAT 1 cut(s) 982
NspI RCATGY 2 cut(s) 509, 1240
NspV TTCGAA 2 cut(s) 448, 952
PaeI GCATGC 1 cut(s) 1240
PagI TCATGA 1 cut(s) 595
PasI CCCWGGG 1 cut(s) 1249
PciI ACATGT 1 cut(s) 505
PcsI WCGNNNNNNNCGW 2 cut(s) 95, 454
PctI GAATGC 1 cut(s) 1273
PdmI GAANNNNTTC 1 cut(s) 958
PfeI GAWTC 5 cut(s) 199, 278, 599, 767, 1254
PfoI TCCNGGA 1 cut(s) 1089
PkrI GCNGC 3 cut(s) 77, 80, 83
PleI GAGTC 6 cut(s) 63, 180, 664, 1231, 1238, 1335
PluTI GGCGCC 1 cut(s) 946
PpsI GAGTC 6 cut(s) 63, 180, 664, 1231, 1238, 1335
PscI ACATGT 1 cut(s) 505
PshBI ATTAAT 1 cut(s) 36
Psp1406I AACGTT 1 cut(s) 549
Psp6I CCWGG 5 cut(s) 242, 342, 1089, 1113, 1248
PspGI CCWGG 5 cut(s) 242, 342, 1089, 1113, 1248
PspN4I GGNNCC 4 cut(s) 171, 248, 944, 1112
PspPI GGNCC 2 cut(s) 131, 1308
PstI CTGCAG 1 cut(s) 303
PsuI RGATCY 2 cut(s) 1102, 1110
RsaI GTAC 5 cut(s) 341, 618, 699, 740, 1353
RsaNI GTAC 5 cut(s) 340, 617, 698, 739, 1352
RseI CAYNNNNRTG 1 cut(s) 471
SaqAI TTAA 6 cut(s) 36, 378, 471, 1056, 1061, 1158
SatI GCNGC 3 cut(s) 76, 79, 82
Sau3AI GATC 5 cut(s) 190, 752, 1102, 1110, 1330
Sau96I GGNCC 2 cut(s) 131, 1308
SchI GAGTC 6 cut(s) 64, 180, 665, 1232, 1238, 1335
ScrFI CCNGG 6 cut(s) 244, 344, 401, 1091, 1115, 1250
SduI GDGCHC 1 cut(s) 152
SfaNI GCATC 7 cut(s) 634, 666, 678, 790, 796, 967, 1117
SfcI CTRYAG 3 cut(s) 70, 299, 612
SfoI GGCGCC 1 cut(s) 944
SfuI TTCGAA 2 cut(s) 448, 952
SmiMI CAYNNNNRTG 1 cut(s) 471
SmlI CTYRAG 1 cut(s) 902
SmoI CTYRAG 1 cut(s) 902
SpeI ACTAGT 1 cut(s) 908
SphI GCATGC 1 cut(s) 1240
Sse9I AATT 9 cut(s) 326, 567, 639, 647, 743, 1057, 1062, 1154, 1167
SsiI CCGC 7 cut(s) 14, 75, 78, 803, 829, 852, 1076
SspDI GGCGCC 1 cut(s) 942
SspI AATATT 2 cut(s) 163, 936
SspMI CTAG 2 cut(s) 909, 1082
StyD4I CCNGG 6 cut(s) 242, 342, 399, 1089, 1113, 1248
TaaI ACNGT 3 cut(s) 437, 488, 1211
TaiI ACGT 2 cut(s) 552, 1025
TaqI TCGA 6 cut(s) 98, 189, 448, 952, 962, 1339
TasI AATT 9 cut(s) 326, 567, 639, 647, 743, 1057, 1062, 1154, 1167
TatI WGTACW 2 cut(s) 738, 1351
TauI GCSGC 2 cut(s) 78, 81
TfiI GAWTC 5 cut(s) 199, 278, 599, 767, 1254
Tru1I TTAA 6 cut(s) 36, 378, 471, 1056, 1061, 1158
Tru9I TTAA 6 cut(s) 36, 378, 471, 1056, 1061, 1158
TseFI GTSAC 2 cut(s) 707, 888
TseI GCWGC 1 cut(s) 81
Tsp45I GTSAC 2 cut(s) 707, 888
TspDTI ATGAA 3 cut(s) 336, 684, 853
VneI GTGCAC 1 cut(s) 148
VspI ATTAAT 1 cut(s) 36
XapI RAATTY 5 cut(s) 326, 639, 743, 1154, 1167
XceI RCATGY 2 cut(s) 509, 1240
XcmI CCANNNNNNNNNTGG 1 cut(s) 1368
XmnI GAANNNNTTC 1 cut(s) 958
XspI CTAG 2 cut(s) 909, 1082
Zsp2I ATGCAT 1 cut(s) 982
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.