Rw1G026710

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0013430
Physical Location & Seq
Reverse (-)
53957049 .. 53961881
4833 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G026710.1

Sequence Viewer

Length: 1395 bp
ATGGGTTATTGGGTATTTGAAACACAGGTGGCAAACCGTAGTATGAGAGACAAAGACTCCAATCCTAATGTCCATGAGAGCCTTTCAAGAAGCTTTGTATCAGTGGAGGAGCCAAAGCCAGAGACAAGGGCAGGGCCTGGCTTGATTTCCTGTACTACTTTCAATATTCTGGCTCCAATCTACAAACGACTCGATCAACTGAATCAAGGGCTTCGTGAGAGTCAATTCAGCTCATTCTGGGTGAATAGGAATCAGAAGATTTTGGATTGGTTACTGTATGAATCGTCTACTATTATATGTCTCCAGGAGTTCTGGGTTGGAAATGAAGAATTTGTGAATATGTACCTGAAGAGGCTTGGTGATGCTGGCTATAGTACCTTCAAGCTTGCCCGAACTAACAACCGTGGAGATGGTCTGCTGACTGCCGTTCGAAGTGATTGCTTTAGAGTTTTAAACTATCGAGAGTTGCACTTTAATGATCTTGGAGACCGTGTTGCTCAGCTATTACATGTTCAGTTAACTGCTCCCTTCTCACAAATCCAGAGAGGCAACCTTCAACAAGAAATTCTTATTGTGAACACTCACTTGTTATTTCCTCATGACTCCAGTCTTTGTATTGTAAGATTGCATCAGGTTTACAAAATTTTGCAATTTGTGGAGGCTTATCAAAACGAAAACAAGCTAAACCATATGCCCATCATACTTTGTGGTGACTGGAATGGAAGCAAGCGTGGTCATGTGTACAAGTTTCTTAGATCACAGGGGTTTGAATCAACATATGACACTGCTCATCAATATACTGACAATGATGCCCACAAGTGGGTTAGCCACCGCAACCATAGGGGAAACATCTGTGGAGTCGACTTCATTTGGCTTTGTAATCCAAACAAGTCCCGTAAACCATTAAAGACCAGTTGGTGTGAAGCTGTTTTTGGAATATTAAGGCGCCAGCTTCGAAAAGCTTCAATGGCTGAAAATGATGCATTTGCCTTTTTGAAGGGCGACAACCATGGCGATTTTATTACTTCCACTGCTTTCTGTGAAGCACTCCATCAGGTTAATTTAATTGGTCATCCATCCGGACTGGGTTTCCAGGAGACGAGGGATCTCTGGATCCAAGCAGACACAGATGCAAATGGTGTCCTTGATTATGAAGAATTCAAGAATATGATCTGGAGTTGTACAGGGTCAGACCAAAAGGAAAGCCTCACTGGGAGTAGAGAGGAGTCTGAGGAAGGCACCAAGGAAGCCCTTGGATTCAAGGTGAAGAATGCAGTTCTTTACCCAAGTGAGGCGGAGAGGGGAATATGGCCTGAAAATTATACACTTTCGGATCATGCTAGACTGACTGTTGTACTTTCACCAGAAAACATGTGGTGTTGTTCTCAGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_8 Exo_endo_phos

Protein Analysis

464

Amino Acids

53.3

Weight (kDa)

6.25

Isoelectric Point (pI)

40.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 80 - 308 2.2e-08 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013430)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02270 AT1G02270
fragaria_vesca FvH4_7g18080 FvH4_7g18080 FvH4_7g18080
malus_domestica MD01G1095200.v1.1 MD07G1163500.v1.1 MD07G1164200.v1.1
prunus_persica Prupe.2G203200_v2.0.a1
pyrus_communis pycom01g11740 pycom07g15930
rosa_chinensis RchiOBHm_Chr1g0361711
rosa_laevigata RLG00000027708
rosa_multiflora Rmu_sc0003426.1_g000004
rosa_rugosa Rorug01G0291000
rosa_samantha Rh1BG264200 Rh1DG294200
rosa_wichuraiana Rw1G026710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 945, 1238
AccI GTMKAC 2 cut(s) 287, 861
AccIII TCCGGA 1 cut(s) 1079
AciI CCGC 2 cut(s) 832, 1295
AclWI GGATC 4 cut(s) 1108, 1113, 1121, 1341
AcsI RAATTY 4 cut(s) 329, 564, 642, 1157
AcuI CTGAAG 1 cut(s) 368
AcyI GRCGYC 1 cut(s) 946
AfaI GTAC 6 cut(s) 154, 344, 376, 743, 1183, 1356
AfiI CCNNNNNNNGG 3 cut(s) 819, 820, 1291
AflIII ACRYGT 2 cut(s) 508, 1371
AjnI CCWGG 3 cut(s) 136, 303, 1092
AjuI GAANNNNNNNTTGG 2 cut(s) 915, 947
AluBI AGCT 8 cut(s) 93, 231, 385, 502, 682, 926, 952, 962
AluI AGCT 8 cut(s) 93, 231, 385, 502, 682, 926, 952, 962
Alw26I GTCTC 5 cut(s) 42, 116, 305, 480, 1091
AlwI GGATC 4 cut(s) 1108, 1113, 1121, 1341
AlwNI CAGNNNCTG 1 cut(s) 137
Aor13HI TCCGGA 1 cut(s) 1079
AoxI GGCC 2 cut(s) 134, 1310
ApoI RAATTY 4 cut(s) 329, 564, 642, 1157
Asp700I GAANNNNTTC 1 cut(s) 961
AspLEI GCGC 1 cut(s) 948
AspS9I GGNCC 1 cut(s) 134
AsuHPI GGTGA 5 cut(s) 253, 371, 722, 1276, 1353
AsuII TTCGAA 2 cut(s) 430, 955
BamHI GGATCC 1 cut(s) 1113
BanI GGYRCC 2 cut(s) 945, 1238
BccI CCATC 4 cut(s) 404, 704, 1059, 1084
BceAI ACGGC 1 cut(s) 410
BcgI CGANNNNNNTGC 2 cut(s) 420, 454
BciT130I CCWGG 3 cut(s) 138, 305, 1094
BcoDI GTCTC 5 cut(s) 42, 116, 305, 480, 1091
BfaI CTAG 1 cut(s) 1341
BfmI CTRYAG 1 cut(s) 370
BfoI RGCGCY 1 cut(s) 949
BlpI GCTNAGC 1 cut(s) 498
Bme1390I CCNGG 3 cut(s) 138, 305, 1094
BmgT120I GGNCC 1 cut(s) 134
BmiI GGNNCC 5 cut(s) 111, 174, 947, 1115, 1240
BmrFI CCNGG 3 cut(s) 138, 305, 1094
BmrI ACTGGG 2 cut(s) 1094, 1221
BmsI GCATC 5 cut(s) 352, 637, 799, 970, 1120
BmuI ACTGGG 2 cut(s) 1094, 1221
BoxI GACNNNNGTC 1 cut(s) 606
BpmI CTGGAG 3 cut(s) 287, 589, 1195
Bpu1102I GCTNAGC 1 cut(s) 498
Bpu14I TTCGAA 2 cut(s) 430, 955
BsaHI GRCGYC 1 cut(s) 946
BsaI GGTCTC 1 cut(s) 480
BsaJI CCNNGG 4 cut(s) 403, 1009, 1242, 1252
BsaWI WCCGGW 1 cut(s) 1079
BsaXI ACNNNNNCTCC 4 cut(s) 41, 71, 477, 507
Bsc4I CCNNNNNNNGG 3 cut(s) 819, 820, 1291
Bse1I ACTGG 5 cut(s) 606, 719, 912, 1089, 1216
BseAI TCCGGA 1 cut(s) 1079
BseBI CCWGG 3 cut(s) 138, 305, 1094
BseDI CCNNGG 4 cut(s) 403, 1009, 1242, 1252
BseGI GGATG 2 cut(s) 1072, 1076
BseLI CCNNNNNNNGG 3 cut(s) 819, 820, 1291
BseMII CTCAG 2 cut(s) 512, 1221
BseNI ACTGG 5 cut(s) 606, 719, 912, 1089, 1216
BseRI GAGGAG 2 cut(s) 122, 1238
BshFI GGCC 2 cut(s) 136, 1312
BshNI GGYRCC 2 cut(s) 945, 1238
BsiSI CCGG 1 cut(s) 1080
BslFI GGGAC 1 cut(s) 877
BslI CCNNNNNNNGG 3 cut(s) 819, 820, 1291
BsmAI GTCTC 5 cut(s) 42, 116, 305, 480, 1091
BsmBI CGTCTC 1 cut(s) 1091
BsmFI GGGAC 1 cut(s) 877
BsmI GAATGC 1 cut(s) 1276
BsnI GGCC 2 cut(s) 136, 1312
Bso31I GGTCTC 1 cut(s) 480
Bsp119I TTCGAA 2 cut(s) 430, 955
Bsp13I TCCGGA 1 cut(s) 1079
Bsp1407I TGTACA 2 cut(s) 741, 1181
Bsp143I GATC 7 cut(s) 193, 478, 755, 1105, 1113, 1170, 1333
Bsp1720I GCTNAGC 1 cut(s) 498
Bsp19I CCATGG 1 cut(s) 1009
BspACI CCGC 2 cut(s) 832, 1295
BspANI GGCC 2 cut(s) 136, 1312
BspCNI CTCAG 2 cut(s) 511, 1222
BspEI TCCGGA 1 cut(s) 1079
BspHI TCATGA 1 cut(s) 598
BspLI GGNNCC 5 cut(s) 111, 174, 947, 1115, 1240
BspPI GGATC 4 cut(s) 1108, 1113, 1121, 1341
BspT104I TTCGAA 2 cut(s) 430, 955
BspT107I GGYRCC 2 cut(s) 945, 1238
BspTNI GGTCTC 1 cut(s) 480
BsrGI TGTACA 2 cut(s) 741, 1181
BsrI ACTGG 5 cut(s) 606, 719, 912, 1089, 1216
BssECI CCNNGG 4 cut(s) 403, 1009, 1242, 1252
BssMI GATC 7 cut(s) 193, 478, 755, 1105, 1113, 1170, 1333
BssNI GRCGYC 1 cut(s) 946
BssT1I CCWWGG 3 cut(s) 1009, 1242, 1252
Bst2UI CCWGG 3 cut(s) 138, 305, 1094
Bst4CI ACNGT 5 cut(s) 38, 276, 404, 491, 1351
Bst6I CTCTTC 1 cut(s) 344
BstACI GRCGYC 1 cut(s) 946
BstAUI TGTACA 2 cut(s) 741, 1181
BstBI TTCGAA 2 cut(s) 430, 955
BstC8I GCNNGC 4 cut(s) 367, 387, 728, 950
BstDEI CTNAG 4 cut(s) 498, 752, 1230, 1386
BstDSI CCRYGG 2 cut(s) 403, 1009
BstF5I GGATG 2 cut(s) 1072, 1076
BstH2I RGCGCY 1 cut(s) 949
BstHHI GCGC 1 cut(s) 948
BstKTI GATC 7 cut(s) 196, 481, 758, 1108, 1116, 1173, 1336
BstMAI GTCTC 5 cut(s) 42, 116, 305, 480, 1091
BstMBI GATC 7 cut(s) 193, 478, 755, 1105, 1113, 1170, 1333
BstMWI GCNNNNNNNGC 1 cut(s) 968
BstNI CCWGG 3 cut(s) 138, 305, 1094
BstNSI RCATGY 2 cut(s) 512, 1375
BstPAI GACNNNNGTC 1 cut(s) 606
BstSCI CCNGG 3 cut(s) 136, 303, 1092
BstSFI CTRYAG 1 cut(s) 370
BstX2I RGATCY 2 cut(s) 1105, 1113
BstYI RGATCY 2 cut(s) 1105, 1113
BsuRI GGCC 2 cut(s) 136, 1312
BtgI CCRYGG 2 cut(s) 403, 1009
BtsCI GGATG 2 cut(s) 1072, 1076
BtsI GCAGTG 2 cut(s) 783, 1029
BtsIMutI CAGTG 4 cut(s) 108, 783, 1029, 1209
Cac8I GCNNGC 4 cut(s) 367, 387, 728, 950
CaiI CAGNNNCTG 1 cut(s) 137
CciI TCATGA 1 cut(s) 598
CfoI GCGC 1 cut(s) 948
Cfr13I GGNCC 1 cut(s) 134
Csp6I GTAC 6 cut(s) 153, 343, 375, 742, 1182, 1355
CviAII CATG 7 cut(s) 74, 509, 599, 737, 1010, 1337, 1372
CviQI GTAC 6 cut(s) 153, 343, 375, 742, 1182, 1355
DdeI CTNAG 4 cut(s) 498, 752, 1230, 1386
DinI GGCGCC 1 cut(s) 947
DpnI GATC 7 cut(s) 195, 480, 757, 1107, 1115, 1172, 1335
DpnII GATC 7 cut(s) 193, 478, 755, 1105, 1113, 1170, 1333
DraI TTTAAA 1 cut(s) 453
Eam1104I CTCTTC 1 cut(s) 344
EarI CTCTTC 1 cut(s) 344
EciI GGCGGA 1 cut(s) 1310
Eco130I CCWWGG 3 cut(s) 1009, 1242, 1252
Eco31I GGTCTC 1 cut(s) 480
Eco57I CTGAAG 1 cut(s) 368
EcoO109I RGGNCCY 1 cut(s) 134
EcoRI GAATTC 1 cut(s) 1157
EcoRII CCWGG 3 cut(s) 136, 303, 1092
EcoT14I CCWWGG 3 cut(s) 1009, 1242, 1252
EcoT22I ATGCAT 1 cut(s) 985
EgeI GGCGCC 1 cut(s) 947
EheI GGCGCC 1 cut(s) 947
ErhI CCWWGG 3 cut(s) 1009, 1242, 1252
Esp3I CGTCTC 1 cut(s) 1091
FaeI CATG 7 cut(s) 77, 512, 602, 740, 1013, 1340, 1375
FalI AAGNNNNNCTT 2 cut(s) 552, 584
FaqI GGGAC 1 cut(s) 877
FatI CATG 7 cut(s) 73, 508, 598, 736, 1009, 1336, 1371
FauNDI CATATG 2 cut(s) 690, 778
FblI GTMKAC 2 cut(s) 287, 861
FokI GGATG 2 cut(s) 1059, 1063
FspBI CTAG 1 cut(s) 1341
GlaI GCGC 1 cut(s) 947
GsuI CTGGAG 3 cut(s) 287, 589, 1195
HaeII RGCGCY 1 cut(s) 949
HaeIII GGCC 2 cut(s) 136, 1312
HapII CCGG 1 cut(s) 1080
HhaI GCGC 1 cut(s) 948
Hin1I GRCGYC 1 cut(s) 946
Hin1II CATG 7 cut(s) 77, 512, 602, 740, 1013, 1340, 1375
Hin6I GCGC 1 cut(s) 946
HinP1I GCGC 1 cut(s) 946
HincII GTYRAC 2 cut(s) 519, 862
HindII GTYRAC 2 cut(s) 519, 862
HindIII AAGCTT 3 cut(s) 91, 383, 960
HpaI GTTAAC 1 cut(s) 519
HpaII CCGG 1 cut(s) 1080
HphI GGTGA 5 cut(s) 253, 371, 722, 1276, 1353
Hpy166II GTNNAC 7 cut(s) 288, 519, 577, 637, 742, 862, 899
Hpy188I TCNGA 4 cut(s) 255, 1192, 1231, 1333
Hpy188III TCNNGA 9 cut(s) 87, 215, 461, 541, 599, 1080, 1111, 1162, 1174
Hpy8I GTNNAC 7 cut(s) 288, 519, 577, 637, 742, 862, 899
HpyAV CCTTC 5 cut(s) 388, 538, 563, 991, 1229
HpyCH4III ACNGT 5 cut(s) 38, 276, 404, 491, 1351
HpyCH4V TGCA 6 cut(s) 469, 628, 649, 983, 1133, 1274
HpyF10VI GCNNNNNNNGC 1 cut(s) 968
HpyF3I CTNAG 4 cut(s) 498, 752, 1230, 1386
Hsp92I GRCGYC 1 cut(s) 946
Hsp92II CATG 7 cut(s) 77, 512, 602, 740, 1013, 1340, 1375
HspAI GCGC 1 cut(s) 946
KasI GGCGCC 1 cut(s) 945
Kpn2I TCCGGA 1 cut(s) 1079
KspAI GTTAAC 1 cut(s) 519
Kzo9I GATC 7 cut(s) 193, 478, 755, 1105, 1113, 1170, 1333
LmnI GCTCC 3 cut(s) 109, 178, 529
LweI GCATC 5 cut(s) 352, 637, 799, 970, 1120
MaeI CTAG 1 cut(s) 1341
MaeIII GTNAC 2 cut(s) 270, 710
MalI GATC 7 cut(s) 195, 480, 757, 1107, 1115, 1172, 1335
MboI GATC 7 cut(s) 193, 478, 755, 1105, 1113, 1170, 1333
MboII GAAGA 5 cut(s) 268, 338, 361, 1166, 1279
MflI RGATCY 2 cut(s) 1105, 1113
MluCI AATT 9 cut(s) 224, 329, 564, 642, 650, 1060, 1065, 1157, 1318
Mly113I GGCGCC 1 cut(s) 946
MlyI GAGTC 6 cut(s) 50, 183, 229, 596, 867, 1235
MmeI TCCRAC 1 cut(s) 298
Mph1103I ATGCAT 1 cut(s) 985
MroI TCCGGA 1 cut(s) 1079
MroXI GAANNNNTTC 1 cut(s) 961
MseI TTAA 7 cut(s) 452, 474, 518, 905, 941, 1059, 1064
MslI CAYNNNNRTG 1 cut(s) 474
MspI CCGG 1 cut(s) 1080
MspR9I CCNGG 3 cut(s) 138, 305, 1094
Mva1269I GAATGC 1 cut(s) 1276
MvaI CCWGG 3 cut(s) 138, 305, 1094
MwoI GCNNNNNNNGC 1 cut(s) 968
NarI GGCGCC 1 cut(s) 946
NcoI CCATGG 1 cut(s) 1009
NdeI CATATG 2 cut(s) 690, 778
NdeII GATC 7 cut(s) 193, 478, 755, 1105, 1113, 1170, 1333
NlaIII CATG 7 cut(s) 77, 512, 602, 740, 1013, 1340, 1375
NlaIV GGNNCC 5 cut(s) 111, 174, 947, 1115, 1240
NmuCI GTSAC 1 cut(s) 710
NsiI ATGCAT 1 cut(s) 985
NspI RCATGY 2 cut(s) 512, 1375
NspV TTCGAA 2 cut(s) 430, 955
PagI TCATGA 1 cut(s) 598
PciI ACATGT 2 cut(s) 508, 1371
PctI GAATGC 1 cut(s) 1276
PdmI GAANNNNTTC 1 cut(s) 961
PfeI GAWTC 5 cut(s) 202, 250, 281, 770, 1257
PfoI TCCNGGA 2 cut(s) 303, 1092
PleI GAGTC 6 cut(s) 50, 183, 228, 596, 866, 1234
PluTI GGCGCC 1 cut(s) 949
PpsI GAGTC 6 cut(s) 50, 183, 228, 596, 866, 1234
PscI ACATGT 2 cut(s) 508, 1371
PshAI GACNNNNGTC 1 cut(s) 606
Psp6I CCWGG 3 cut(s) 136, 303, 1092
PspGI CCWGG 3 cut(s) 136, 303, 1092
PspN4I GGNNCC 5 cut(s) 111, 174, 947, 1115, 1240
PspPI GGNCC 1 cut(s) 134
PstNI CAGNNNCTG 1 cut(s) 137
PsuI RGATCY 2 cut(s) 1105, 1113
RsaI GTAC 6 cut(s) 154, 344, 376, 743, 1183, 1356
RsaNI GTAC 6 cut(s) 153, 343, 375, 742, 1182, 1355
RseI CAYNNNNRTG 1 cut(s) 474
SalI GTCGAC 1 cut(s) 860
SaqAI TTAA 7 cut(s) 452, 474, 518, 905, 941, 1059, 1064
Sau3AI GATC 7 cut(s) 193, 478, 755, 1105, 1113, 1170, 1333
Sau96I GGNCC 1 cut(s) 134
SchI GAGTC 6 cut(s) 50, 183, 229, 596, 867, 1235
ScrFI CCNGG 3 cut(s) 138, 305, 1094
SfaNI GCATC 5 cut(s) 352, 637, 799, 970, 1120
SfcI CTRYAG 1 cut(s) 370
SfoI GGCGCC 1 cut(s) 947
SfuI TTCGAA 2 cut(s) 430, 955
SmiMI CAYNNNNRTG 1 cut(s) 474
Sse9I AATT 9 cut(s) 224, 329, 564, 642, 650, 1060, 1065, 1157, 1318
SsiI CCGC 2 cut(s) 832, 1295
SspDI GGCGCC 1 cut(s) 945
SspI AATATT 2 cut(s) 166, 939
SspMI CTAG 1 cut(s) 1341
StyD4I CCNGG 3 cut(s) 136, 303, 1092
StyI CCWWGG 3 cut(s) 1009, 1242, 1252
TaaI ACNGT 5 cut(s) 38, 276, 404, 491, 1351
TaqI TCGA 5 cut(s) 192, 430, 460, 861, 955
TasI AATT 9 cut(s) 224, 329, 564, 642, 650, 1060, 1065, 1157, 1318
TatI WGTACW 4 cut(s) 152, 741, 1181, 1354
TfiI GAWTC 5 cut(s) 202, 250, 281, 770, 1257
Tru1I TTAA 7 cut(s) 452, 474, 518, 905, 941, 1059, 1064
Tru9I TTAA 7 cut(s) 452, 474, 518, 905, 941, 1059, 1064
TscAI CASTG 4 cut(s) 108, 790, 1036, 1216
TseFI GTSAC 1 cut(s) 710
Tsp45I GTSAC 1 cut(s) 710
TspDTI ATGAA 4 cut(s) 294, 339, 856, 1167
TspRI CASTG 4 cut(s) 108, 790, 1036, 1216
XapI RAATTY 4 cut(s) 329, 564, 642, 1157
XceI RCATGY 2 cut(s) 512, 1375
XcmI CCANNNNNNNNNTGG 1 cut(s) 1371
XmiI GTMKAC 2 cut(s) 287, 861
XmnI GAANNNNTTC 1 cut(s) 961
XspI CTAG 1 cut(s) 1341
Zsp2I ATGCAT 1 cut(s) 985
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.