RLG00000027708

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0013430
Physical Location & Seq
Forward (+)
14211252 .. 14214573
3322 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027708

Sequence Viewer

Length: 1371 bp
ATGGTGGCAAACCGTAGTATGAGAGACAAAGACTCCAATCCTAATGTCCGTGATAGCCTTCCAAGAAGCTTTGTATCAGTGGAGGAGCCAAAGCCAGAGACAAGGGCAGGGCCTGGCTTGATTTCCTGTACTACTTTCAATATTCTGGCTCCAATCTACAAACGACTCGATCAACAGAATCAAGAGCTTCGTGAGAGTCAATTCAGCTCATTCTGGGTGACTAGGAATCAGAAGATTTTGGATTGGTTACTGTATGAATCGTCTACTATTATATGTCTCCAGGAGTTCTGGGTTGGAAATGAAGAATTTGTGAATATGTACCTGAAGAGGCTTGGTGATGCTGGCTATAGTACCTTCAAGCTTGCCCGAACTAACAACCGTGGAGATGGTCTGCTGACTGCCGTTCGAAGTGATTGCTTTAGAGTTCTAAACTATCGAGAGTTGCACTTTAATGATCTTGGAGACCGTGTTGCTCAGCTATTACATGTTCAGTTAACTGCTCCCTTCTCACAAATCCAGAGAGGCAACCTTCAACAAGAAATTCTTATTGTGAACACTCACTTGTTATTTCCTCATGATTCCAGTCTTTGTATTGTAAGACTGCATCAGGTTTACAAAATTTTGCAATTTGTGGAGGCCTATCAAAATGAAAACAAGCTAAACCATATGCCCATCATACTCTGTGGTGACTGGAATGGAAGCAAGCGTGGTCATGTGTACAAGTTTCTTAGATCACAGGGGTTTGAATCAACATATGACACTGCTCATCAATATACTGACAATGATGCCCACAAGTGGGTTAGCCACCGCAACCATATGGGAAACATCTGTGGAGTCGACTTCATTTGGCTTTGTAATCCTAACATGTCCCGTAAACCATTAAAGACCAGTTGGTGTGAAGCTGTTTTTGGAATATTAAGGCGCCAGCTTCGAAAAGCTTCGATGGCTGAAAATGATGCATTTGCCTTTTTGAAGGGCGACAACCATGGTGATTTTATTACTTCCTCTGCTTTCTGTGAAGCACTCCATCAGGTTAATTTAATTGGTCATCCATCCGGACTGGGTTTCCAGGAGACGAGGGATCTCTGGATCCAAGCAGACACAGACGCAAATGGTGTCCTTGATTATGAAGAATTCAAGAATATGATCCGGAGTTGTACAGGGTCAGACCAAAAGGAAAGCCTCACTGGCAGTAGAGAGGAGTCTGAGGAAGGCACCAAGGAAGCCCTTGGATTCAAGGTGAAGAATGCAGTTCTTTACCCAAGTGAGGCGGAGAGGGGAATGTGGCCTGAAAATTATACACTTTCAGATCATGCTAGACTGACCGTTGTACTTTCACCAGAAAACATGTGGTGTTGTTCTCAGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_8 Exo_endo_phos

Protein Analysis

457

Amino Acids

52.33

Weight (kDa)

6.22

Isoelectric Point (pI)

45.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 72 - 301 2.5e-08 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013430)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02270 AT1G02270
fragaria_vesca FvH4_7g18080 FvH4_7g18080 FvH4_7g18080
malus_domestica MD01G1095200.v1.1 MD07G1163500.v1.1 MD07G1164200.v1.1
prunus_persica Prupe.2G203200_v2.0.a1
pyrus_communis pycom01g11740 pycom07g15930
rosa_chinensis RchiOBHm_Chr1g0361711
rosa_laevigata RLG00000027708
rosa_multiflora Rmu_sc0003426.1_g000004
rosa_rugosa Rorug01G0291000
rosa_samantha Rh1BG264200 Rh1DG294200
rosa_wichuraiana Rw1G026710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 921, 1214
AccI GTMKAC 2 cut(s) 263, 837
AccIII TCCGGA 2 cut(s) 1055, 1149
AciI CCGC 2 cut(s) 808, 1271
AclWI GGATC 4 cut(s) 1084, 1089, 1097, 1141
AcsI RAATTY 4 cut(s) 305, 540, 618, 1133
AcuI CTGAAG 1 cut(s) 344
AcyI GRCGYC 1 cut(s) 922
AfaI GTAC 6 cut(s) 130, 320, 352, 719, 1159, 1332
AfiI CCNNNNNNNGG 3 cut(s) 795, 796, 1267
AflIII ACRYGT 3 cut(s) 484, 864, 1347
AgsI TTSAA 7 cut(s) 139, 358, 533, 746, 973, 1138, 1237
AjnI CCWGG 3 cut(s) 112, 279, 1068
AjuI GAANNNNNNNTTGG 2 cut(s) 891, 923
AluBI AGCT 9 cut(s) 69, 187, 207, 361, 478, 658, 902, 928, 938
AluI AGCT 9 cut(s) 69, 187, 207, 361, 478, 658, 902, 928, 938
Alw26I GTCTC 5 cut(s) 18, 92, 281, 456, 1067
AlwI GGATC 4 cut(s) 1084, 1089, 1097, 1141
AlwNI CAGNNNCTG 1 cut(s) 113
Aor13HI TCCGGA 2 cut(s) 1055, 1149
AoxI GGCC 3 cut(s) 110, 636, 1286
ApoI RAATTY 4 cut(s) 305, 540, 618, 1133
Asp700I GAANNNNTTC 1 cut(s) 937
AspLEI GCGC 1 cut(s) 924
AspS9I GGNCC 1 cut(s) 110
AsuHPI GGTGA 6 cut(s) 229, 347, 698, 1001, 1252, 1329
AsuII TTCGAA 2 cut(s) 406, 931
BamHI GGATCC 1 cut(s) 1089
BanI GGYRCC 2 cut(s) 921, 1214
BccI CCATC 5 cut(s) 380, 680, 937, 1035, 1060
BceAI ACGGC 1 cut(s) 386
BcgI CGANNNNNNTGC 2 cut(s) 396, 430
BciT130I CCWGG 3 cut(s) 114, 281, 1070
BcoDI GTCTC 5 cut(s) 18, 92, 281, 456, 1067
BfaI CTAG 2 cut(s) 222, 1317
BfmI CTRYAG 1 cut(s) 346
BfoI RGCGCY 1 cut(s) 925
BglI GCCNNNNNGGC 1 cut(s) 1188
BlpI GCTNAGC 1 cut(s) 474
Bme1390I CCNGG 3 cut(s) 114, 281, 1070
BmgT120I GGNCC 1 cut(s) 110
BmiI GGNNCC 5 cut(s) 87, 150, 923, 1091, 1216
BmrFI CCNGG 3 cut(s) 114, 281, 1070
BmrI ACTGGG 1 cut(s) 1070
BmsI GCATC 4 cut(s) 328, 613, 775, 946
BmuI ACTGGG 1 cut(s) 1070
BpmI CTGGAG 1 cut(s) 263
Bpu1102I GCTNAGC 1 cut(s) 474
Bpu14I TTCGAA 2 cut(s) 406, 931
BsaHI GRCGYC 1 cut(s) 922
BsaI GGTCTC 1 cut(s) 456
BsaJI CCNNGG 4 cut(s) 379, 985, 1218, 1228
BsaWI WCCGGW 2 cut(s) 1055, 1149
BsaXI ACNNNNNCTCC 4 cut(s) 17, 47, 453, 483
Bsc4I CCNNNNNNNGG 3 cut(s) 795, 796, 1267
Bse1I ACTGG 5 cut(s) 582, 695, 888, 1065, 1192
BseAI TCCGGA 2 cut(s) 1055, 1149
BseBI CCWGG 3 cut(s) 114, 281, 1070
BseDI CCNNGG 4 cut(s) 379, 985, 1218, 1228
BseGI GGATG 2 cut(s) 1048, 1052
BseLI CCNNNNNNNGG 3 cut(s) 795, 796, 1267
BseMII CTCAG 2 cut(s) 488, 1197
BseNI ACTGG 5 cut(s) 582, 695, 888, 1065, 1192
BseRI GAGGAG 2 cut(s) 98, 1214
BshFI GGCC 3 cut(s) 112, 638, 1288
BshNI GGYRCC 2 cut(s) 921, 1214
BsiSI CCGG 2 cut(s) 1056, 1150
BslFI GGGAC 1 cut(s) 853
BslI CCNNNNNNNGG 3 cut(s) 795, 796, 1267
BsmAI GTCTC 5 cut(s) 18, 92, 281, 456, 1067
BsmBI CGTCTC 1 cut(s) 1067
BsmFI GGGAC 1 cut(s) 853
BsmI GAATGC 1 cut(s) 1252
BsnI GGCC 3 cut(s) 112, 638, 1288
Bso31I GGTCTC 1 cut(s) 456
Bsp119I TTCGAA 2 cut(s) 406, 931
Bsp13I TCCGGA 2 cut(s) 1055, 1149
Bsp1407I TGTACA 2 cut(s) 717, 1157
Bsp143I GATC 7 cut(s) 169, 454, 731, 1081, 1089, 1146, 1309
Bsp1720I GCTNAGC 1 cut(s) 474
Bsp19I CCATGG 1 cut(s) 985
BspACI CCGC 2 cut(s) 808, 1271
BspANI GGCC 3 cut(s) 112, 638, 1288
BspCNI CTCAG 2 cut(s) 487, 1198
BspEI TCCGGA 2 cut(s) 1055, 1149
BspHI TCATGA 1 cut(s) 574
BspLI GGNNCC 5 cut(s) 87, 150, 923, 1091, 1216
BspPI GGATC 4 cut(s) 1084, 1089, 1097, 1141
BspT104I TTCGAA 2 cut(s) 406, 931
BspT107I GGYRCC 2 cut(s) 921, 1214
BspTNI GGTCTC 1 cut(s) 456
BsrGI TGTACA 2 cut(s) 717, 1157
BsrI ACTGG 5 cut(s) 582, 695, 888, 1065, 1192
BssECI CCNNGG 4 cut(s) 379, 985, 1218, 1228
BssMI GATC 7 cut(s) 169, 454, 731, 1081, 1089, 1146, 1309
BssNI GRCGYC 1 cut(s) 922
BssT1I CCWWGG 3 cut(s) 985, 1218, 1228
Bst2UI CCWGG 3 cut(s) 114, 281, 1070
Bst4CI ACNGT 5 cut(s) 14, 252, 380, 467, 1327
Bst6I CTCTTC 1 cut(s) 320
BstACI GRCGYC 1 cut(s) 922
BstAUI TGTACA 2 cut(s) 717, 1157
BstBI TTCGAA 2 cut(s) 406, 931
BstC8I GCNNGC 4 cut(s) 343, 363, 704, 926
BstDEI CTNAG 4 cut(s) 474, 728, 1206, 1362
BstDSI CCRYGG 2 cut(s) 379, 985
BstF5I GGATG 2 cut(s) 1048, 1052
BstH2I RGCGCY 1 cut(s) 925
BstHHI GCGC 1 cut(s) 924
BstKTI GATC 7 cut(s) 172, 457, 734, 1084, 1092, 1149, 1312
BstMAI GTCTC 5 cut(s) 18, 92, 281, 456, 1067
BstMBI GATC 7 cut(s) 169, 454, 731, 1081, 1089, 1146, 1309
BstMWI GCNNNNNNNGC 2 cut(s) 944, 1188
BstNI CCWGG 3 cut(s) 114, 281, 1070
BstNSI RCATGY 3 cut(s) 488, 868, 1351
BstSCI CCNGG 3 cut(s) 112, 279, 1068
BstSFI CTRYAG 1 cut(s) 346
BstX2I RGATCY 2 cut(s) 1081, 1089
BstYI RGATCY 2 cut(s) 1081, 1089
BsuRI GGCC 3 cut(s) 112, 638, 1288
BtgI CCRYGG 2 cut(s) 379, 985
BtsCI GGATG 2 cut(s) 1048, 1052
BtsI GCAGTG 1 cut(s) 759
BtsIMutI CAGTG 3 cut(s) 84, 759, 1185
Cac8I GCNNGC 4 cut(s) 343, 363, 704, 926
CaiI CAGNNNCTG 1 cut(s) 113
CciI TCATGA 1 cut(s) 574
CfoI GCGC 1 cut(s) 924
Cfr13I GGNCC 1 cut(s) 110
CseI GACGC 1 cut(s) 1115
Csp6I GTAC 6 cut(s) 129, 319, 351, 718, 1158, 1331
CviAII CATG 7 cut(s) 485, 575, 713, 865, 986, 1313, 1348
CviQI GTAC 6 cut(s) 129, 319, 351, 718, 1158, 1331
DdeI CTNAG 4 cut(s) 474, 728, 1206, 1362
DinI GGCGCC 1 cut(s) 923
DpnI GATC 7 cut(s) 171, 456, 733, 1083, 1091, 1148, 1311
DpnII GATC 7 cut(s) 169, 454, 731, 1081, 1089, 1146, 1309
Eam1104I CTCTTC 1 cut(s) 320
EarI CTCTTC 1 cut(s) 320
EciI GGCGGA 1 cut(s) 1286
Eco130I CCWWGG 3 cut(s) 985, 1218, 1228
Eco147I AGGCCT 1 cut(s) 638
Eco31I GGTCTC 1 cut(s) 456
Eco57I CTGAAG 1 cut(s) 344
EcoO109I RGGNCCY 1 cut(s) 110
EcoRI GAATTC 1 cut(s) 1133
EcoRII CCWGG 3 cut(s) 112, 279, 1068
EcoT14I CCWWGG 3 cut(s) 985, 1218, 1228
EcoT22I ATGCAT 1 cut(s) 961
EgeI GGCGCC 1 cut(s) 923
EheI GGCGCC 1 cut(s) 923
ErhI CCWWGG 3 cut(s) 985, 1218, 1228
Esp3I CGTCTC 1 cut(s) 1067
FaeI CATG 7 cut(s) 488, 578, 716, 868, 989, 1316, 1351
FalI AAGNNNNNCTT 2 cut(s) 528, 560
FaqI GGGAC 1 cut(s) 853
FatI CATG 7 cut(s) 484, 574, 712, 864, 985, 1312, 1347
FauNDI CATATG 3 cut(s) 666, 754, 816
FblI GTMKAC 2 cut(s) 263, 837
FokI GGATG 2 cut(s) 1035, 1039
FspBI CTAG 2 cut(s) 222, 1317
GlaI GCGC 1 cut(s) 923
GsuI CTGGAG 1 cut(s) 263
HaeII RGCGCY 1 cut(s) 925
HaeIII GGCC 3 cut(s) 112, 638, 1288
HapII CCGG 2 cut(s) 1056, 1150
HgaI GACGC 1 cut(s) 1115
HhaI GCGC 1 cut(s) 924
Hin1I GRCGYC 1 cut(s) 922
Hin1II CATG 7 cut(s) 488, 578, 716, 868, 989, 1316, 1351
Hin6I GCGC 1 cut(s) 922
HinP1I GCGC 1 cut(s) 922
HincII GTYRAC 2 cut(s) 495, 838
HindII GTYRAC 2 cut(s) 495, 838
HindIII AAGCTT 3 cut(s) 67, 359, 936
HpaI GTTAAC 1 cut(s) 495
HpaII CCGG 2 cut(s) 1056, 1150
HphI GGTGA 6 cut(s) 229, 347, 698, 1001, 1252, 1329
Hpy166II GTNNAC 7 cut(s) 264, 495, 553, 613, 718, 838, 875
Hpy188I TCNGA 4 cut(s) 231, 1168, 1207, 1309
Hpy188III TCNNGA 9 cut(s) 182, 191, 437, 517, 575, 1056, 1087, 1138, 1150
Hpy8I GTNNAC 7 cut(s) 264, 495, 553, 613, 718, 838, 875
HpyAV CCTTC 6 cut(s) 68, 364, 514, 539, 967, 1205
HpyCH4III ACNGT 5 cut(s) 14, 252, 380, 467, 1327
HpyCH4V TGCA 5 cut(s) 445, 604, 625, 959, 1250
HpyF10VI GCNNNNNNNGC 2 cut(s) 944, 1188
HpyF3I CTNAG 4 cut(s) 474, 728, 1206, 1362
Hsp92I GRCGYC 1 cut(s) 922
Hsp92II CATG 7 cut(s) 488, 578, 716, 868, 989, 1316, 1351
HspAI GCGC 1 cut(s) 922
KasI GGCGCC 1 cut(s) 921
Kpn2I TCCGGA 2 cut(s) 1055, 1149
KspAI GTTAAC 1 cut(s) 495
Kzo9I GATC 7 cut(s) 169, 454, 731, 1081, 1089, 1146, 1309
LmnI GCTCC 3 cut(s) 85, 154, 505
LweI GCATC 4 cut(s) 328, 613, 775, 946
MaeI CTAG 2 cut(s) 222, 1317
MaeIII GTNAC 3 cut(s) 217, 246, 686
MalI GATC 7 cut(s) 171, 456, 733, 1083, 1091, 1148, 1311
MboI GATC 7 cut(s) 169, 454, 731, 1081, 1089, 1146, 1309
MboII GAAGA 5 cut(s) 244, 314, 337, 1142, 1255
MflI RGATCY 2 cut(s) 1081, 1089
MluCI AATT 9 cut(s) 200, 305, 540, 618, 626, 1036, 1041, 1133, 1294
Mly113I GGCGCC 1 cut(s) 922
MlyI GAGTC 5 cut(s) 26, 159, 205, 843, 1211
MmeI TCCRAC 1 cut(s) 274
Mph1103I ATGCAT 1 cut(s) 961
MroI TCCGGA 2 cut(s) 1055, 1149
MroXI GAANNNNTTC 1 cut(s) 937
MseI TTAA 6 cut(s) 450, 494, 881, 917, 1035, 1040
MslI CAYNNNNRTG 1 cut(s) 450
MspI CCGG 2 cut(s) 1056, 1150
MspR9I CCNGG 3 cut(s) 114, 281, 1070
Mva1269I GAATGC 1 cut(s) 1252
MvaI CCWGG 3 cut(s) 114, 281, 1070
MwoI GCNNNNNNNGC 2 cut(s) 944, 1188
NarI GGCGCC 1 cut(s) 922
NcoI CCATGG 1 cut(s) 985
NdeI CATATG 3 cut(s) 666, 754, 816
NdeII GATC 7 cut(s) 169, 454, 731, 1081, 1089, 1146, 1309
NlaIII CATG 7 cut(s) 488, 578, 716, 868, 989, 1316, 1351
NlaIV GGNNCC 5 cut(s) 87, 150, 923, 1091, 1216
NmuCI GTSAC 2 cut(s) 217, 686
NsiI ATGCAT 1 cut(s) 961
NspI RCATGY 3 cut(s) 488, 868, 1351
NspV TTCGAA 2 cut(s) 406, 931
PagI TCATGA 1 cut(s) 574
PceI AGGCCT 1 cut(s) 638
PciI ACATGT 3 cut(s) 484, 864, 1347
PctI GAATGC 1 cut(s) 1252
PdmI GAANNNNTTC 1 cut(s) 937
PfeI GAWTC 6 cut(s) 178, 226, 257, 578, 746, 1233
PfoI TCCNGGA 2 cut(s) 279, 1068
PleI GAGTC 5 cut(s) 26, 159, 204, 842, 1210
PluTI GGCGCC 1 cut(s) 925
PpsI GAGTC 5 cut(s) 26, 159, 204, 842, 1210
PscI ACATGT 3 cut(s) 484, 864, 1347
Psp6I CCWGG 3 cut(s) 112, 279, 1068
PspGI CCWGG 3 cut(s) 112, 279, 1068
PspN4I GGNNCC 5 cut(s) 87, 150, 923, 1091, 1216
PspPI GGNCC 1 cut(s) 110
PstNI CAGNNNCTG 1 cut(s) 113
PsuI RGATCY 2 cut(s) 1081, 1089
RsaI GTAC 6 cut(s) 130, 320, 352, 719, 1159, 1332
RsaNI GTAC 6 cut(s) 129, 319, 351, 718, 1158, 1331
RseI CAYNNNNRTG 1 cut(s) 450
SalI GTCGAC 1 cut(s) 836
SaqAI TTAA 6 cut(s) 450, 494, 881, 917, 1035, 1040
Sau3AI GATC 7 cut(s) 169, 454, 731, 1081, 1089, 1146, 1309
Sau96I GGNCC 1 cut(s) 110
SchI GAGTC 5 cut(s) 26, 159, 205, 843, 1211
ScrFI CCNGG 3 cut(s) 114, 281, 1070
SfaNI GCATC 4 cut(s) 328, 613, 775, 946
SfcI CTRYAG 1 cut(s) 346
SfoI GGCGCC 1 cut(s) 923
SfuI TTCGAA 2 cut(s) 406, 931
SmiMI CAYNNNNRTG 1 cut(s) 450
Sse9I AATT 9 cut(s) 200, 305, 540, 618, 626, 1036, 1041, 1133, 1294
SseBI AGGCCT 1 cut(s) 638
SsiI CCGC 2 cut(s) 808, 1271
SspDI GGCGCC 1 cut(s) 921
SspI AATATT 2 cut(s) 142, 915
SspMI CTAG 2 cut(s) 222, 1317
StuI AGGCCT 1 cut(s) 638
StyD4I CCNGG 3 cut(s) 112, 279, 1068
StyI CCWWGG 3 cut(s) 985, 1218, 1228
TaaI ACNGT 5 cut(s) 14, 252, 380, 467, 1327
TaqI TCGA 6 cut(s) 168, 406, 436, 837, 931, 941
TasI AATT 9 cut(s) 200, 305, 540, 618, 626, 1036, 1041, 1133, 1294
TatI WGTACW 4 cut(s) 128, 717, 1157, 1330
TfiI GAWTC 6 cut(s) 178, 226, 257, 578, 746, 1233
Tru1I TTAA 6 cut(s) 450, 494, 881, 917, 1035, 1040
Tru9I TTAA 6 cut(s) 450, 494, 881, 917, 1035, 1040
TscAI CASTG 3 cut(s) 84, 766, 1192
TseFI GTSAC 2 cut(s) 217, 686
Tsp45I GTSAC 2 cut(s) 217, 686
TspDTI ATGAA 5 cut(s) 270, 315, 663, 832, 1143
TspGWI ACGGA 1 cut(s) 38
TspRI CASTG 3 cut(s) 84, 766, 1192
XapI RAATTY 4 cut(s) 305, 540, 618, 1133
XceI RCATGY 3 cut(s) 488, 868, 1351
XcmI CCANNNNNNNNNTGG 1 cut(s) 1347
XmiI GTMKAC 2 cut(s) 263, 837
XmnI GAANNNNTTC 1 cut(s) 937
XspI CTAG 2 cut(s) 222, 1317
Zsp2I ATGCAT 1 cut(s) 961
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.