Rmu_sc0003426.1_g000004

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003426.1
OG0013430
Physical Location & Seq
Forward (+)
13085 .. 18881
5797 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003426.1_g000004.1.cds

Sequence Viewer

Length: 1557 bp
atgctatcggtgtccatcagtggttatggtcatggctcatgcgataatgcgagtgccgagcctcgctggagccggccagcagttattggagccggaaaccttggtggtgtcacatacatttatggatttattgccgcaaatttaacgactttatcatcattaagaacagttgcaaggagttttaacatggtggcaaaccgtagtatgagagacaaagactccaatcctaatgtccatgagagcctttcaagaagctttgtatcagtggaggagccaaagccagagacaagggcagggcctggcttgatttcctgtactactttcaatattctggctccaatctacaaacgactcgatcaactgaatcaagggcttcgtgagagtgaattcagctcattctgggtgactaggaatcaaaagattttggattggttactgtatgattcgtctactattatatgtctccaggagttctgggttggaaatgaagaatttgtgaatatgtacctgaagaggcttggtgatgctggctatagtaccttcaagcttgcccgaactaataaccgtggagatggtctgctgactgccgttcgaagtgattgctttagagttttaaactatcgagagttgcactttaatgatcttggagaccgtgttgctcagctattacatgttcagttaactgctcccttctcacaaatccagagaggcaaccttcaacaagaaattcttattgtgaacactcacttgttatttcctcatgactccagtctttgtattgtaagattgcatcaggtttacaaaattttgcaatttgtggaggcttatcaaaacgaaaacaagctaaaccatatgcccatcatactttgtggtgactggaatggaagcaagcgtggtcatgtgtacaagtttcttagatcacaggggtttgaatcaacatatgacactgctcatcaatatactgacaatgatgcccacaagtgggttagccaccgcaaccataggggaaacatctgtggagtcgacttcatttggctttgtaatccaaacaagtcccgtaaaccattaaagaccagttggtgtgaagctgtttttggaatattaaggcgccagcttcgaaaagcttcgatggctgaaaatgatgcatttgcctttttgaagggcgacaaccatggtgattttattacttcctttgctttctgtgaagcactccatcaggttaatttaattggtcatccatccggactgggtttccaggagacgagggatctctggatccaagcagacacagatgcaaatggtgtccttgattatgaagaattcaagaatatgatctggagttgtacagggtcagaccaaaaggaaagcctcactgggagtagagaggagtctgaggaaggcaccaaggaagcccttggattcgaggtgatgaatgcagttctttacccaagtgaggcggagaggggaatatggcctgaaaattatacactttcggatcatgctagactgactgttgtactttcaccagaaaacatgtggtgttgttctcggtcataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_8 Exo_endo_phos

Protein Analysis

518

Amino Acids

58.76

Weight (kDa)

6.29

Isoelectric Point (pI)

43.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013430)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02270 AT1G02270
fragaria_vesca FvH4_7g18080 FvH4_7g18080 FvH4_7g18080
malus_domestica MD01G1095200.v1.1 MD07G1163500.v1.1 MD07G1164200.v1.1
prunus_persica Prupe.2G203200_v2.0.a1
pyrus_communis pycom01g11740 pycom07g15930
rosa_chinensis RchiOBHm_Chr1g0361711
rosa_laevigata RLG00000027708
rosa_multiflora Rmu_sc0003426.1_g000004
rosa_rugosa Rorug01G0291000
rosa_samantha Rh1BG264200 Rh1DG294200
rosa_wichuraiana Rw1G026710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1107, 1400
AccI GTMKAC 2 cut(s) 449, 1023
AccIII TCCGGA 1 cut(s) 1241
AciI CCGC 3 cut(s) 135, 994, 1457
AclWI GGATC 4 cut(s) 1270, 1275, 1283, 1503
AcoI YGGCCR 1 cut(s) 74
AcsI RAATTY 6 cut(s) 139, 386, 491, 726, 804, 1319
AcuI CTGAAG 1 cut(s) 530
AcyI GRCGYC 1 cut(s) 1108
AfaI GTAC 6 cut(s) 316, 506, 538, 905, 1345, 1518
AfiI CCNNNNNNNGG 3 cut(s) 981, 982, 1453
AflIII ACRYGT 2 cut(s) 670, 1533
AgsI TTSAA 7 cut(s) 249, 325, 544, 719, 932, 1159, 1324
AjnI CCWGG 3 cut(s) 298, 465, 1254
AjuI GAANNNNNNNTTGG 2 cut(s) 1077, 1109
AluBI AGCT 8 cut(s) 255, 393, 547, 664, 844, 1088, 1114, 1124
AluI AGCT 8 cut(s) 255, 393, 547, 664, 844, 1088, 1114, 1124
Alw26I GTCTC 5 cut(s) 204, 278, 467, 642, 1253
AlwI GGATC 4 cut(s) 1270, 1275, 1283, 1503
AlwNI CAGNNNCTG 1 cut(s) 299
Aor13HI TCCGGA 1 cut(s) 1241
AoxI GGCC 3 cut(s) 74, 296, 1472
ApoI RAATTY 6 cut(s) 139, 386, 491, 726, 804, 1319
Asp700I GAANNNNTTC 1 cut(s) 1123
AspLEI GCGC 1 cut(s) 1110
AspS9I GGNCC 1 cut(s) 296
AsuHPI GGTGA 6 cut(s) 415, 533, 884, 1187, 1438, 1515
AsuII TTCGAA 2 cut(s) 592, 1117
BamHI GGATCC 1 cut(s) 1275
BanI GGYRCC 2 cut(s) 1107, 1400
BccI CCATC 6 cut(s) 23, 566, 866, 1123, 1221, 1246
BceAI ACGGC 1 cut(s) 572
BcgI CGANNNNNNTGC 2 cut(s) 582, 616
BciT130I CCWGG 3 cut(s) 300, 467, 1256
BcoDI GTCTC 5 cut(s) 204, 278, 467, 642, 1253
BfaI CTAG 2 cut(s) 408, 1503
BfmI CTRYAG 1 cut(s) 532
BfoI RGCGCY 1 cut(s) 1111
BisI GCNGC 1 cut(s) 135
BlpI GCTNAGC 1 cut(s) 660
BlsI GCNGC 1 cut(s) 136
Bme1390I CCNGG 3 cut(s) 300, 467, 1256
BmgT120I GGNCC 1 cut(s) 296
BmiI GGNNCC 7 cut(s) 71, 91, 273, 336, 1109, 1277, 1402
BmrFI CCNGG 3 cut(s) 300, 467, 1256
BmrI ACTGGG 2 cut(s) 1256, 1383
BmsI GCATC 5 cut(s) 514, 799, 961, 1132, 1282
BmuI ACTGGG 2 cut(s) 1256, 1383
BoxI GACNNNNGTC 1 cut(s) 768
BpmI CTGGAG 4 cut(s) 88, 449, 751, 1357
Bpu1102I GCTNAGC 1 cut(s) 660
Bpu14I TTCGAA 2 cut(s) 592, 1117
BsaHI GRCGYC 1 cut(s) 1108
BsaI GGTCTC 1 cut(s) 642
BsaJI CCNNGG 5 cut(s) 100, 565, 1171, 1404, 1414
BsaWI WCCGGW 1 cut(s) 1241
BsaXI ACNNNNNCTCC 4 cut(s) 203, 233, 639, 669
Bsc4I CCNNNNNNNGG 3 cut(s) 981, 982, 1453
Bse118I RCCGGY 1 cut(s) 72
Bse1I ACTGG 5 cut(s) 768, 881, 1074, 1251, 1378
BseAI TCCGGA 1 cut(s) 1241
BseBI CCWGG 3 cut(s) 300, 467, 1256
BseDI CCNNGG 5 cut(s) 100, 565, 1171, 1404, 1414
BseGI GGATG 2 cut(s) 1234, 1238
BseLI CCNNNNNNNGG 3 cut(s) 981, 982, 1453
BseMII CTCAG 2 cut(s) 674, 1383
BseNI ACTGG 5 cut(s) 768, 881, 1074, 1251, 1378
BseRI GAGGAG 2 cut(s) 284, 1400
BshFI GGCC 3 cut(s) 76, 298, 1474
BshNI GGYRCC 2 cut(s) 1107, 1400
BsiSI CCGG 3 cut(s) 73, 93, 1242
BslFI GGGAC 1 cut(s) 1039
BslI CCNNNNNNNGG 3 cut(s) 981, 982, 1453
BsmAI GTCTC 5 cut(s) 204, 278, 467, 642, 1253
BsmBI CGTCTC 1 cut(s) 1253
BsmFI GGGAC 1 cut(s) 1039
BsmI GAATGC 1 cut(s) 1438
BsnI GGCC 3 cut(s) 76, 298, 1474
Bso31I GGTCTC 1 cut(s) 642
Bsp119I TTCGAA 2 cut(s) 592, 1117
Bsp13I TCCGGA 1 cut(s) 1241
Bsp1407I TGTACA 2 cut(s) 903, 1343
Bsp143I GATC 7 cut(s) 355, 640, 917, 1267, 1275, 1332, 1495
Bsp1720I GCTNAGC 1 cut(s) 660
Bsp19I CCATGG 1 cut(s) 1171
BspACI CCGC 3 cut(s) 135, 994, 1457
BspANI GGCC 3 cut(s) 76, 298, 1474
BspCNI CTCAG 2 cut(s) 673, 1384
BspEI TCCGGA 1 cut(s) 1241
BspHI TCATGA 1 cut(s) 760
BspLI GGNNCC 7 cut(s) 71, 91, 273, 336, 1109, 1277, 1402
BspPI GGATC 4 cut(s) 1270, 1275, 1283, 1503
BspT104I TTCGAA 2 cut(s) 592, 1117
BspT107I GGYRCC 2 cut(s) 1107, 1400
BspTNI GGTCTC 1 cut(s) 642
BsrFI RCCGGY 1 cut(s) 72
BsrGI TGTACA 2 cut(s) 903, 1343
BsrI ACTGG 5 cut(s) 768, 881, 1074, 1251, 1378
BssAI RCCGGY 1 cut(s) 72
BssECI CCNNGG 5 cut(s) 100, 565, 1171, 1404, 1414
BssMI GATC 7 cut(s) 355, 640, 917, 1267, 1275, 1332, 1495
BssNI GRCGYC 1 cut(s) 1108
BssT1I CCWWGG 4 cut(s) 100, 1171, 1404, 1414
Bst2UI CCWGG 3 cut(s) 300, 467, 1256
Bst4CI ACNGT 6 cut(s) 169, 200, 438, 566, 653, 1513
Bst6I CTCTTC 1 cut(s) 506
BstACI GRCGYC 1 cut(s) 1108
BstAUI TGTACA 2 cut(s) 903, 1343
BstBI TTCGAA 2 cut(s) 592, 1117
BstC8I GCNNGC 6 cut(s) 74, 78, 529, 549, 890, 1112
BstDEI CTNAG 3 cut(s) 660, 914, 1392
BstDSI CCRYGG 2 cut(s) 565, 1171
BstF5I GGATG 2 cut(s) 1234, 1238
BstH2I RGCGCY 1 cut(s) 1111
BstHHI GCGC 1 cut(s) 1110
BstKTI GATC 7 cut(s) 358, 643, 920, 1270, 1278, 1335, 1498
BstMAI GTCTC 5 cut(s) 204, 278, 467, 642, 1253
BstMBI GATC 7 cut(s) 355, 640, 917, 1267, 1275, 1332, 1495
BstMWI GCNNNNNNNGC 1 cut(s) 1130
BstNI CCWGG 3 cut(s) 300, 467, 1256
BstNSI RCATGY 2 cut(s) 674, 1537
BstPAI GACNNNNGTC 1 cut(s) 768
BstSCI CCNGG 3 cut(s) 298, 465, 1254
BstSFI CTRYAG 1 cut(s) 532
BstX2I RGATCY 2 cut(s) 1267, 1275
BstYI RGATCY 2 cut(s) 1267, 1275
BsuRI GGCC 3 cut(s) 76, 298, 1474
BtgI CCRYGG 2 cut(s) 565, 1171
BtsCI GGATG 2 cut(s) 1234, 1238
BtsI GCAGTG 1 cut(s) 945
BtsIMutI CAGTG 4 cut(s) 25, 270, 945, 1371
Cac8I GCNNGC 6 cut(s) 74, 78, 529, 549, 890, 1112
CaiI CAGNNNCTG 1 cut(s) 299
CciI TCATGA 1 cut(s) 760
CfoI GCGC 1 cut(s) 1110
Cfr10I RCCGGY 1 cut(s) 72
Cfr13I GGNCC 1 cut(s) 296
Csp6I GTAC 6 cut(s) 315, 505, 537, 904, 1344, 1517
CviQI GTAC 6 cut(s) 315, 505, 537, 904, 1344, 1517
DdeI CTNAG 3 cut(s) 660, 914, 1392
DinI GGCGCC 1 cut(s) 1109
DpnI GATC 7 cut(s) 357, 642, 919, 1269, 1277, 1334, 1497
DpnII GATC 7 cut(s) 355, 640, 917, 1267, 1275, 1332, 1495
DraI TTTAAA 1 cut(s) 615
EaeI YGGCCR 1 cut(s) 74
Eam1104I CTCTTC 1 cut(s) 506
EarI CTCTTC 1 cut(s) 506
EciI GGCGGA 1 cut(s) 1472
Eco130I CCWWGG 4 cut(s) 100, 1171, 1404, 1414
Eco31I GGTCTC 1 cut(s) 642
Eco57I CTGAAG 1 cut(s) 530
EcoO109I RGGNCCY 1 cut(s) 296
EcoRI GAATTC 2 cut(s) 386, 1319
EcoRII CCWGG 3 cut(s) 298, 465, 1254
EcoT14I CCWWGG 4 cut(s) 100, 1171, 1404, 1414
EcoT22I ATGCAT 1 cut(s) 1147
EgeI GGCGCC 1 cut(s) 1109
EheI GGCGCC 1 cut(s) 1109
ErhI CCWWGG 4 cut(s) 100, 1171, 1404, 1414
Esp3I CGTCTC 1 cut(s) 1253
FalI AAGNNNNNCTT 2 cut(s) 714, 746
FaqI GGGAC 1 cut(s) 1039
FauNDI CATATG 2 cut(s) 852, 940
FblI GTMKAC 2 cut(s) 449, 1023
Fnu4HI GCNGC 1 cut(s) 135
FokI GGATG 2 cut(s) 1221, 1225
Fsp4HI GCNGC 1 cut(s) 135
FspBI CTAG 2 cut(s) 408, 1503
GlaI GCGC 1 cut(s) 1109
GluI GCNGC 1 cut(s) 135
GsuI CTGGAG 4 cut(s) 88, 449, 751, 1357
HaeII RGCGCY 1 cut(s) 1111
HaeIII GGCC 3 cut(s) 76, 298, 1474
HapII CCGG 3 cut(s) 73, 93, 1242
HhaI GCGC 1 cut(s) 1110
Hin1I GRCGYC 1 cut(s) 1108
Hin6I GCGC 1 cut(s) 1108
HinP1I GCGC 1 cut(s) 1108
HincII GTYRAC 2 cut(s) 681, 1024
HindII GTYRAC 2 cut(s) 681, 1024
HindIII AAGCTT 3 cut(s) 253, 545, 1122
HpaI GTTAAC 1 cut(s) 681
HpaII CCGG 3 cut(s) 73, 93, 1242
HphI GGTGA 6 cut(s) 415, 533, 884, 1187, 1438, 1515
Hpy166II GTNNAC 7 cut(s) 450, 681, 739, 799, 904, 1024, 1061
Hpy188I TCNGA 3 cut(s) 1354, 1393, 1495
Hpy188III TCNNGA 9 cut(s) 249, 377, 623, 703, 761, 1242, 1273, 1324, 1336
Hpy8I GTNNAC 7 cut(s) 450, 681, 739, 799, 904, 1024, 1061
HpyAV CCTTC 5 cut(s) 550, 700, 725, 1153, 1391
HpyCH4III ACNGT 6 cut(s) 169, 200, 438, 566, 653, 1513
HpyCH4V TGCA 7 cut(s) 173, 631, 790, 811, 1145, 1295, 1436
HpyF10VI GCNNNNNNNGC 1 cut(s) 1130
HpyF3I CTNAG 3 cut(s) 660, 914, 1392
Hsp92I GRCGYC 1 cut(s) 1108
HspAI GCGC 1 cut(s) 1108
KasI GGCGCC 1 cut(s) 1107
Kpn2I TCCGGA 1 cut(s) 1241
KroI GCCGGC 1 cut(s) 72
KroNI GCCGGC 1 cut(s) 74
KspAI GTTAAC 1 cut(s) 681
Kzo9I GATC 7 cut(s) 355, 640, 917, 1267, 1275, 1332, 1495
LmnI GCTCC 5 cut(s) 69, 89, 271, 340, 691
LweI GCATC 5 cut(s) 514, 799, 961, 1132, 1282
MaeI CTAG 2 cut(s) 408, 1503
MaeIII GTNAC 4 cut(s) 109, 403, 432, 872
MalI GATC 7 cut(s) 357, 642, 919, 1269, 1277, 1334, 1497
MboI GATC 7 cut(s) 355, 640, 917, 1267, 1275, 1332, 1495
MboII GAAGA 3 cut(s) 500, 523, 1328
MflI RGATCY 2 cut(s) 1267, 1275
Mly113I GGCGCC 1 cut(s) 1108
MlyI GAGTC 5 cut(s) 212, 345, 758, 1029, 1397
MmeI TCCRAC 1 cut(s) 460
Mph1103I ATGCAT 1 cut(s) 1147
MroI TCCGGA 1 cut(s) 1241
MroNI GCCGGC 1 cut(s) 72
MroXI GAANNNNTTC 1 cut(s) 1123
MslI CAYNNNNRTG 1 cut(s) 636
MspI CCGG 3 cut(s) 73, 93, 1242
MspR9I CCNGG 3 cut(s) 300, 467, 1256
Mva1269I GAATGC 1 cut(s) 1438
MvaI CCWGG 3 cut(s) 300, 467, 1256
MwoI GCNNNNNNNGC 1 cut(s) 1130
NaeI GCCGGC 1 cut(s) 74
NarI GGCGCC 1 cut(s) 1108
NcoI CCATGG 1 cut(s) 1171
NdeI CATATG 2 cut(s) 852, 940
NdeII GATC 7 cut(s) 355, 640, 917, 1267, 1275, 1332, 1495
NgoMIV GCCGGC 1 cut(s) 72
NlaIV GGNNCC 7 cut(s) 71, 91, 273, 336, 1109, 1277, 1402
NmeAIII GCCGAG 1 cut(s) 82
NmuCI GTSAC 3 cut(s) 109, 403, 872
NsiI ATGCAT 1 cut(s) 1147
NspI RCATGY 2 cut(s) 674, 1537
NspV TTCGAA 2 cut(s) 592, 1117
PagI TCATGA 1 cut(s) 760
PciI ACATGT 2 cut(s) 670, 1533
PctI GAATGC 1 cut(s) 1438
PdiI GCCGGC 1 cut(s) 74
PdmI GAANNNNTTC 1 cut(s) 1123
PfeI GAWTC 5 cut(s) 364, 412, 443, 932, 1419
PfoI TCCNGGA 2 cut(s) 465, 1254
PkrI GCNGC 1 cut(s) 136
PleI GAGTC 5 cut(s) 212, 345, 758, 1028, 1396
PluTI GGCGCC 1 cut(s) 1111
PpsI GAGTC 5 cut(s) 212, 345, 758, 1028, 1396
PscI ACATGT 2 cut(s) 670, 1533
PshAI GACNNNNGTC 1 cut(s) 768
Psp6I CCWGG 3 cut(s) 298, 465, 1254
PspGI CCWGG 3 cut(s) 298, 465, 1254
PspN4I GGNNCC 7 cut(s) 71, 91, 273, 336, 1109, 1277, 1402
PspPI GGNCC 1 cut(s) 296
PstNI CAGNNNCTG 1 cut(s) 299
PsuI RGATCY 2 cut(s) 1267, 1275
RsaI GTAC 6 cut(s) 316, 506, 538, 905, 1345, 1518
RsaNI GTAC 6 cut(s) 315, 505, 537, 904, 1344, 1517
RseI CAYNNNNRTG 1 cut(s) 636
SalI GTCGAC 1 cut(s) 1022
SatI GCNGC 1 cut(s) 135
Sau3AI GATC 7 cut(s) 355, 640, 917, 1267, 1275, 1332, 1495
Sau96I GGNCC 1 cut(s) 296
SchI GAGTC 5 cut(s) 212, 345, 758, 1029, 1397
ScrFI CCNGG 3 cut(s) 300, 467, 1256
SfaNI GCATC 5 cut(s) 514, 799, 961, 1132, 1282
SfcI CTRYAG 1 cut(s) 532
SfoI GGCGCC 1 cut(s) 1109
SfuI TTCGAA 2 cut(s) 592, 1117
SmiMI CAYNNNNRTG 1 cut(s) 636
SsiI CCGC 3 cut(s) 135, 994, 1457
SspDI GGCGCC 1 cut(s) 1107
SspI AATATT 2 cut(s) 328, 1101
SspMI CTAG 2 cut(s) 408, 1503
StyD4I CCNGG 3 cut(s) 298, 465, 1254
StyI CCWWGG 4 cut(s) 100, 1171, 1404, 1414
TaaI ACNGT 6 cut(s) 169, 200, 438, 566, 653, 1513
TaqI TCGA 7 cut(s) 354, 592, 622, 1023, 1117, 1127, 1422
TaqII GACCGA 1 cut(s) 1539
TatI WGTACW 4 cut(s) 314, 903, 1343, 1516
TauI GCSGC 1 cut(s) 137
TfiI GAWTC 5 cut(s) 364, 412, 443, 932, 1419
TscAI CASTG 4 cut(s) 25, 270, 952, 1378
TseFI GTSAC 3 cut(s) 109, 403, 872
Tsp45I GTSAC 3 cut(s) 109, 403, 872
TspDTI ATGAA 4 cut(s) 501, 1018, 1329, 1445
TspRI CASTG 4 cut(s) 25, 270, 952, 1378
XapI RAATTY 6 cut(s) 139, 386, 491, 726, 804, 1319
XceI RCATGY 2 cut(s) 674, 1537
XcmI CCANNNNNNNNNTGG 1 cut(s) 1533
XmiI GTMKAC 2 cut(s) 449, 1023
XmnI GAANNNNTTC 1 cut(s) 1123
XspI CTAG 2 cut(s) 408, 1503
Zsp2I ATGCAT 1 cut(s) 1147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.