MD07G1163500.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0013430
Physical Location & Seq
Reverse (-)
23855538 .. 23858747
3210 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1163500.v1.1.491

Sequence Viewer

Length: 1242 bp
ATGGATTTGGTTTCTGAGAAAATGGGTACTCAGGAAATCGTTTATTGGATAATGGATACTGAAAATTTTGAGACGTTGCAGGGTTTAGGTGCGGGTTCTAATGTGCCAAAGATTAATCGCAGAGAAAGTGAGAGCCTTTCAAGGAGCTACAGCGGCAGCTGCATATCGTTGCCGTCGATAGCAGAGCTAAAGCCAGAGACTTTGGCAGGGCCTTGCTTAGTCTCGTGCATGACTTTCAATATTTTGGCTCCAATCTACAAAAGACTCGATCAACAGAATCAAAGCCTTAGCGAGAGCGACTACGGGGCGTTTTGGGTGGCCAGGAACCACAGGATTTTGGATTGGTTGCTCTGTGAATCATCTGCCTTTATTTGTCTGCAGTTCTGGGTTGGAAATGAAGAATTTGTGAATATGTACCTGGACAGGCTTGGTGATGCAGGCTATACTACCTTTAAGCTCGCCCGAACTAACAACCGGGGAGATGGTTTGCTGACTGCTTTGAGGAGGGACTGTTTTAGTGTTCGAAACTGCCAAGAGTTGCATTTTAATGATTTTGGAGACCGTGTTGCTCAGCTATTACATGTTCAGTTAGCTGCCCCATTTTCACGAAAGAGGAAACGTTCAACAAGAAATGCTAATTGTGAATACTCATTTGCTGTTTACAAAATTTTGCAATTTGTGAAGTCATATCAGAATGAAAAGAAGCTAAACCCGATGCCCATCGTACTCTGTGGTGACTGGAATGGAAGTAAGCGAGGCCATGTGTACAAATTCCTTAGATCACAGGGTTTTGAATCAACATATGATACTGCTCATCAATATACCAATGCGGATGCTCACAAGCGCCAGCTTCGAAAAGCTTCGATGGCTGAAAATGATGCATTTGCCTTTCTGAAGGGTGATAGTCATGGTGATTTTATTACCTCCTCAGCTTTCTGTTTCCAGGAGACAAGGGATCTCTGGATCCAGGCAGACGCCGATGCAAATGGTGTTCTTGATTACGAAGAATTTAAGAATAGAATTTGGAGTTCGAGAGCGTCAGAGGATAAGGAAAGTCTCAACGGTAGTAGAGAGGAGTCCAAACGAGGCATGCAGGACTCCCTGGGATTCAATGTGAAGAATGCAGTTCTATATCCCCGTGAGGTGGAGAAGGGAATATGGCCCGAAGATTATACTCTCTCTGATCACGCTCGACTCTCGGTTGTACTTTCACCAGAAAGGATGTGGTGTTGTAAGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_8 Exo_endo_phos

Protein Analysis

414

Amino Acids

47.26

Weight (kDa)

6.48

Isoelectric Point (pI)

45.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013430)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02270 AT1G02270
fragaria_vesca FvH4_7g18080 FvH4_7g18080 FvH4_7g18080
malus_domestica MD01G1095200.v1.1 MD07G1163500.v1.1 MD07G1164200.v1.1
prunus_persica Prupe.2G203200_v2.0.a1
pyrus_communis pycom01g11740 pycom07g15930
rosa_chinensis RchiOBHm_Chr1g0361711
rosa_laevigata RLG00000027708
rosa_multiflora Rmu_sc0003426.1_g000004
rosa_rugosa Rorug01G0291000
rosa_samantha Rh1BG264200 Rh1DG294200
rosa_wichuraiana Rw1G026710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 92, 153, 830
AclI AACGTT 1 cut(s) 619
AclWI GGATC 3 cut(s) 958, 963, 971
AcoI YGGCCR 1 cut(s) 318
AcsI RAATTY 6 cut(s) 64, 401, 666, 770, 1007, 1020
AcuI CTGAAG 1 cut(s) 914
AcyI GRCGYC 1 cut(s) 975
AfaI GTAC 5 cut(s) 28, 416, 726, 767, 1206
AfiI CCNNNNNNNGG 1 cut(s) 1144
AflIII ACRYGT 1 cut(s) 580
AgsI TTSAA 5 cut(s) 141, 238, 624, 794, 1111
AjnI CCWGG 5 cut(s) 320, 417, 942, 966, 1101
Alw26I GTCTC 6 cut(s) 65, 191, 226, 552, 941, 1061
AlwI GGATC 3 cut(s) 958, 963, 971
AoxI GGCC 4 cut(s) 209, 318, 757, 1160
ApeKI GCWGC 3 cut(s) 156, 159, 593
ApoI RAATTY 6 cut(s) 64, 401, 666, 770, 1007, 1020
AseI ATTAAT 1 cut(s) 114
Asp700I GAANNNNTTC 1 cut(s) 859
AspLEI GCGC 1 cut(s) 846
AspS9I GGNCC 2 cut(s) 209, 1161
AsuC2I CCSGG 1 cut(s) 476
AsuHPI GGTGA 5 cut(s) 443, 746, 911, 923, 1203
AsuII TTCGAA 2 cut(s) 523, 853
BalI TGGCCA 1 cut(s) 320
BamHI GGATCC 1 cut(s) 963
BauI CACGAG 1 cut(s) 223
BbvCI CCTCAGC 1 cut(s) 928
BbvI GCAGC 3 cut(s) 146, 168, 580
BccI CCATC 3 cut(s) 476, 728, 859
BceAI ACGGC 1 cut(s) 157
BciT130I CCWGG 5 cut(s) 322, 419, 944, 968, 1103
BciVI GTATCC 1 cut(s) 49
BclI TGATCA 1 cut(s) 1183
BcnI CCSGG 1 cut(s) 476
BcoDI GTCTC 6 cut(s) 65, 191, 226, 552, 941, 1061
BfmI CTRYAG 2 cut(s) 148, 377
BfoI RGCGCY 1 cut(s) 847
BfuI GTATCC 1 cut(s) 49
BisI GCNGC 4 cut(s) 154, 157, 160, 594
BlpI GCTNAGC 1 cut(s) 570
BlsI GCNGC 4 cut(s) 155, 158, 161, 595
Bme1390I CCNGG 6 cut(s) 322, 419, 476, 944, 968, 1103
BmgT120I GGNCC 2 cut(s) 209, 1161
BmiI GGNNCC 3 cut(s) 249, 326, 965
BmrFI CCNGG 6 cut(s) 322, 419, 476, 944, 968, 1103
BmsI GCATC 5 cut(s) 424, 705, 823, 868, 970
Bpu10I CCTNAGC 2 cut(s) 287, 928
Bpu1102I GCTNAGC 1 cut(s) 570
Bpu14I TTCGAA 2 cut(s) 523, 853
BpuMI CCSGG 1 cut(s) 476
BsaBI GATNNNNATC 1 cut(s) 719
BsaHI GRCGYC 1 cut(s) 975
BsaI GGTCTC 1 cut(s) 552
BsaJI CCNNGG 3 cut(s) 475, 1101, 1102
BsaXI ACNNNNNCTCC 4 cut(s) 496, 526, 549, 579
Bsc4I CCNNNNNNNGG 1 cut(s) 1144
Bse1I ACTGG 1 cut(s) 743
Bse8I GATNNNNATC 1 cut(s) 719
BseBI CCWGG 5 cut(s) 322, 419, 944, 968, 1103
BseDI CCNNGG 3 cut(s) 475, 1101, 1102
BseGI GGATG 2 cut(s) 838, 1227
BseJI GATNNNNATC 1 cut(s) 719
BseLI CCNNNNNNNGG 1 cut(s) 1144
BseMII CTCAG 4 cut(s) 6, 44, 584, 942
BseNI ACTGG 1 cut(s) 743
BseRI GAGGAG 3 cut(s) 517, 916, 1088
BseXI GCAGC 3 cut(s) 146, 168, 580
BshFI GGCC 4 cut(s) 211, 320, 759, 1162
BsiSI CCGG 1 cut(s) 475
BslFI GGGAC 1 cut(s) 521
BslI CCNNNNNNNGG 1 cut(s) 1144
BsmAI GTCTC 6 cut(s) 65, 191, 226, 552, 941, 1061
BsmBI CGTCTC 1 cut(s) 65
BsmFI GGGAC 1 cut(s) 521
BsmI GAATGC 1 cut(s) 1126
BsnI GGCC 4 cut(s) 211, 320, 759, 1162
Bso31I GGTCTC 1 cut(s) 552
Bsp119I TTCGAA 2 cut(s) 523, 853
Bsp1407I TGTACA 1 cut(s) 765
Bsp143I GATC 5 cut(s) 268, 779, 955, 963, 1183
Bsp1720I GCTNAGC 1 cut(s) 570
BspACI CCGC 3 cut(s) 92, 153, 830
BspANI GGCC 4 cut(s) 211, 320, 759, 1162
BspCNI CTCAG 4 cut(s) 7, 43, 583, 941
BspLI GGNNCC 3 cut(s) 249, 326, 965
BspMAI CTGCAG 1 cut(s) 381
BspPI GGATC 3 cut(s) 958, 963, 971
BspT104I TTCGAA 2 cut(s) 523, 853
BspTNI GGTCTC 1 cut(s) 552
BsrGI TGTACA 1 cut(s) 765
BsrI ACTGG 1 cut(s) 743
BssECI CCNNGG 3 cut(s) 475, 1101, 1102
BssMI GATC 5 cut(s) 268, 779, 955, 963, 1183
BssNI GRCGYC 1 cut(s) 975
BssSI CACGAG 1 cut(s) 223
Bst2BI CACGAG 1 cut(s) 223
Bst2UI CCWGG 5 cut(s) 322, 419, 944, 968, 1103
Bst4CI ACNGT 3 cut(s) 512, 563, 1064
BstACI GRCGYC 1 cut(s) 975
BstAUI TGTACA 1 cut(s) 765
BstBI TTCGAA 2 cut(s) 523, 853
BstC8I GCNNGC 4 cut(s) 439, 459, 848, 1091
BstDEI CTNAG 7 cut(s) 15, 30, 217, 287, 570, 776, 928
BstF5I GGATG 2 cut(s) 838, 1227
BstH2I RGCGCY 1 cut(s) 847
BstHHI GCGC 1 cut(s) 846
BstKTI GATC 5 cut(s) 271, 782, 958, 966, 1186
BstMAI GTCTC 6 cut(s) 65, 191, 226, 552, 941, 1061
BstMBI GATC 5 cut(s) 268, 779, 955, 963, 1183
BstMWI GCNNNNNNNGC 3 cut(s) 153, 159, 866
BstNI CCWGG 5 cut(s) 322, 419, 944, 968, 1103
BstNSI RCATGY 2 cut(s) 584, 1093
BstSCI CCNGG 6 cut(s) 320, 417, 474, 942, 966, 1101
BstSFI CTRYAG 2 cut(s) 148, 377
BstV1I GCAGC 3 cut(s) 146, 168, 580
BstX2I RGATCY 2 cut(s) 955, 963
BstYI RGATCY 2 cut(s) 955, 963
BsuI GTATCC 1 cut(s) 49
BsuRI GGCC 4 cut(s) 211, 320, 759, 1162
BtsCI GGATG 2 cut(s) 838, 1227
Cac8I GCNNGC 4 cut(s) 439, 459, 848, 1091
CfoI GCGC 1 cut(s) 846
Cfr13I GGNCC 2 cut(s) 209, 1161
CseI GACGC 2 cut(s) 983, 1026
Csp6I GTAC 5 cut(s) 27, 415, 725, 766, 1205
CviAII CATG 5 cut(s) 229, 581, 761, 908, 1090
CviQI GTAC 5 cut(s) 27, 415, 725, 766, 1205
DdeI CTNAG 7 cut(s) 15, 30, 217, 287, 570, 776, 928
DpnI GATC 5 cut(s) 270, 781, 957, 965, 1185
DpnII GATC 5 cut(s) 268, 779, 955, 963, 1183
EaeI YGGCCR 1 cut(s) 318
Eco31I GGTCTC 1 cut(s) 552
Eco57I CTGAAG 1 cut(s) 914
EcoO109I RGGNCCY 1 cut(s) 209
EcoRII CCWGG 5 cut(s) 320, 417, 942, 966, 1101
EcoT22I ATGCAT 1 cut(s) 883
Esp3I CGTCTC 1 cut(s) 65
FaeI CATG 5 cut(s) 232, 584, 764, 911, 1093
FaqI GGGAC 1 cut(s) 521
FatI CATG 5 cut(s) 228, 580, 760, 907, 1089
FauI CCCGC 1 cut(s) 85
FauNDI CATATG 1 cut(s) 802
FbaI TGATCA 1 cut(s) 1183
Fnu4HI GCNGC 4 cut(s) 154, 157, 160, 594
FokI GGATG 2 cut(s) 845, 1234
Fsp4HI GCNGC 4 cut(s) 154, 157, 160, 594
GlaI GCGC 1 cut(s) 845
GluI GCNGC 4 cut(s) 154, 157, 160, 594
HaeII RGCGCY 1 cut(s) 847
HaeIII GGCC 4 cut(s) 211, 320, 759, 1162
HapII CCGG 1 cut(s) 475
HgaI GACGC 2 cut(s) 983, 1026
HhaI GCGC 1 cut(s) 846
Hin1I GRCGYC 1 cut(s) 975
Hin1II CATG 5 cut(s) 232, 584, 764, 911, 1093
Hin6I GCGC 1 cut(s) 844
HinP1I GCGC 1 cut(s) 844
HindIII AAGCTT 1 cut(s) 858
HinfI GANTC 8 cut(s) 264, 277, 356, 794, 1076, 1097, 1107, 1194
HpaII CCGG 1 cut(s) 475
HphI GGTGA 5 cut(s) 443, 746, 911, 923, 1203
Hpy166II GTNNAC 2 cut(s) 661, 766
Hpy188I TCNGA 5 cut(s) 16, 693, 894, 1042, 1183
Hpy188III TCNNGA 5 cut(s) 32, 606, 961, 995, 1032
Hpy8I GTNNAC 2 cut(s) 661, 766
Hpy99I CGWCG 1 cut(s) 178
HpyAV CCTTC 2 cut(s) 889, 1144
HpyCH4III ACNGT 3 cut(s) 512, 563, 1064
HpyCH4IV ACGT 2 cut(s) 74, 619
HpyF10VI GCNNNNNNNGC 3 cut(s) 153, 159, 866
HpyF3I CTNAG 7 cut(s) 15, 30, 217, 287, 570, 776, 928
HpySE526I ACGT 2 cut(s) 74, 619
Hsp92I GRCGYC 1 cut(s) 975
Hsp92II CATG 5 cut(s) 232, 584, 764, 911, 1093
HspAI GCGC 1 cut(s) 844
Ksp22I TGATCA 1 cut(s) 1183
Kzo9I GATC 5 cut(s) 268, 779, 955, 963, 1183
LmnI GCTCC 2 cut(s) 144, 253
Lsp1109I GCAGC 3 cut(s) 146, 168, 580
LweI GCATC 5 cut(s) 424, 705, 823, 868, 970
MaeII ACGT 2 cut(s) 74, 619
MaeIII GTNAC 1 cut(s) 734
MalI GATC 5 cut(s) 270, 781, 957, 965, 1185
MboI GATC 5 cut(s) 268, 779, 955, 963, 1183
MboII GAAGA 4 cut(s) 410, 1016, 1129, 1178
MflI RGATCY 2 cut(s) 955, 963
MlsI TGGCCA 1 cut(s) 320
MluCI AATT 8 cut(s) 64, 401, 637, 666, 674, 770, 1007, 1020
MluNI TGGCCA 1 cut(s) 320
MlyI GAGTC 4 cut(s) 258, 1085, 1091, 1188
MmeI TCCRAC 1 cut(s) 370
Mox20I TGGCCA 1 cut(s) 320
Mph1103I ATGCAT 1 cut(s) 883
MroXI GAANNNNTTC 1 cut(s) 859
MscI TGGCCA 1 cut(s) 320
MseI TTAA 4 cut(s) 114, 453, 546, 1011
MslI CAYNNNNRTG 1 cut(s) 546
Msp20I TGGCCA 1 cut(s) 320
MspA1I CMGCKG 2 cut(s) 153, 159
MspI CCGG 1 cut(s) 475
MspR9I CCNGG 6 cut(s) 322, 419, 476, 944, 968, 1103
Mva1269I GAATGC 1 cut(s) 1126
MvaI CCWGG 5 cut(s) 322, 419, 944, 968, 1103
MwoI GCNNNNNNNGC 3 cut(s) 153, 159, 866
NciI CCSGG 1 cut(s) 476
NdeI CATATG 1 cut(s) 802
NdeII GATC 5 cut(s) 268, 779, 955, 963, 1183
NlaIII CATG 5 cut(s) 232, 584, 764, 911, 1093
NlaIV GGNNCC 3 cut(s) 249, 326, 965
NmuCI GTSAC 1 cut(s) 734
NsiI ATGCAT 1 cut(s) 883
NspI RCATGY 2 cut(s) 584, 1093
NspV TTCGAA 2 cut(s) 523, 853
PaeI GCATGC 1 cut(s) 1093
PasI CCCWGGG 1 cut(s) 1102
PciI ACATGT 1 cut(s) 580
PcsI WCGNNNNNNNCGW 1 cut(s) 173
PctI GAATGC 1 cut(s) 1126
PdmI GAANNNNTTC 1 cut(s) 859
PfeI GAWTC 4 cut(s) 277, 356, 794, 1107
PfoI TCCNGGA 1 cut(s) 942
PkrI GCNGC 4 cut(s) 155, 158, 161, 595
PleI GAGTC 4 cut(s) 258, 1084, 1091, 1188
PpsI GAGTC 4 cut(s) 258, 1084, 1091, 1188
PscI ACATGT 1 cut(s) 580
PshBI ATTAAT 1 cut(s) 114
Psp1406I AACGTT 1 cut(s) 619
Psp6I CCWGG 5 cut(s) 320, 417, 942, 966, 1101
PspGI CCWGG 5 cut(s) 320, 417, 942, 966, 1101
PspN4I GGNNCC 3 cut(s) 249, 326, 965
PspPI GGNCC 2 cut(s) 209, 1161
PstI CTGCAG 1 cut(s) 381
PsuI RGATCY 2 cut(s) 955, 963
PvuII CAGCTG 1 cut(s) 159
RsaI GTAC 5 cut(s) 28, 416, 726, 767, 1206
RsaNI GTAC 5 cut(s) 27, 415, 725, 766, 1205
RseI CAYNNNNRTG 1 cut(s) 546
SaqAI TTAA 4 cut(s) 114, 453, 546, 1011
SatI GCNGC 4 cut(s) 154, 157, 160, 594
Sau3AI GATC 5 cut(s) 268, 779, 955, 963, 1183
Sau96I GGNCC 2 cut(s) 209, 1161
SchI GAGTC 4 cut(s) 258, 1085, 1091, 1188
ScrFI CCNGG 6 cut(s) 322, 419, 476, 944, 968, 1103
SfaNI GCATC 5 cut(s) 424, 705, 823, 868, 970
SfcI CTRYAG 2 cut(s) 148, 377
SfuI TTCGAA 2 cut(s) 523, 853
SmiMI CAYNNNNRTG 1 cut(s) 546
SphI GCATGC 1 cut(s) 1093
Sse9I AATT 8 cut(s) 64, 401, 637, 666, 674, 770, 1007, 1020
SsiI CCGC 3 cut(s) 92, 153, 830
SspI AATATT 1 cut(s) 241
StyD4I CCNGG 6 cut(s) 320, 417, 474, 942, 966, 1101
TaaI ACNGT 3 cut(s) 512, 563, 1064
TaiI ACGT 2 cut(s) 77, 622
TaqI TCGA 7 cut(s) 176, 267, 523, 853, 863, 1031, 1192
TasI AATT 8 cut(s) 64, 401, 637, 666, 674, 770, 1007, 1020
TatI WGTACW 2 cut(s) 765, 1204
TauI GCSGC 1 cut(s) 156
TfiI GAWTC 4 cut(s) 277, 356, 794, 1107
Tru1I TTAA 4 cut(s) 114, 453, 546, 1011
Tru9I TTAA 4 cut(s) 114, 453, 546, 1011
TseFI GTSAC 1 cut(s) 734
TseI GCWGC 3 cut(s) 156, 159, 593
Tsp45I GTSAC 1 cut(s) 734
TspDTI ATGAA 2 cut(s) 411, 711
VspI ATTAAT 1 cut(s) 114
XapI RAATTY 6 cut(s) 64, 401, 666, 770, 1007, 1020
XceI RCATGY 2 cut(s) 584, 1093
XcmI CCANNNNNNNNNTGG 1 cut(s) 1221
XmnI GAANNNNTTC 1 cut(s) 859
Zsp2I ATGCAT 1 cut(s) 883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.