Rh1BG264200

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0013430
Physical Location & Seq
Reverse (-)
39843945 .. 39847914
3970 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG264200.1

Sequence Viewer

Length: 1359 bp
ATGGTGGCAAACCGTAGTATGAGAGACAAAGACTCCAATCCTAATGTCCATGAGAGCCTTTCAAGAAGCTTTGTATCAATGGAGGAGCCAAAGCCAGGGCCTGGCTTGATTTCCTGTACTACTTTCAATATTCTGGCTCCAATCTACAAACGACTCGACCAACAGAATCAAGGGCTTCGTGAGAGTCAATTCAGCTCATTCTGGGTGACTAGGAATCAGAAGATTTTGGATTGGTTACTGTATGAATCGTCTACTATTATATGTCTCCAGGAGTTCTGGGTTGGAAATGAAGAATTTGTGAATATGTACCTGAAGAGGCTTGGTGATGCTGGCTATAGTACCTTCAAGCTTGCCCGAACTAACAACCGTGGAGATGGTCTGCTGACTGCCGTTCGAAGTGATTGCTTTAGAGTTCTAAACTATCGAGAGTTGCGCTTTAATGATTTTGGAGACCGTGTTGCTCAGCTATTACATGTTCAGTTAATTGCTCCCTTCTCACAAATCCAGAGAGGCAACCTTCAACAAGAAATTCTTATTGTGAACACTCACTTGTTATTCCCTCATGATTCCAGTCTTTGTATTGTAAGACTGGATCAGGTTTACAAAATTTTGCAATTTGTGGAGGCCTATCAAAATGAAAACAAGCTAAACCATATGCCCATCATACTCTGTGGTGACTGGAATGGAAGCAAGCGTGGTCATGTGTACAAGTTTCTTAGATCACAAGGGTTTGAATCAACATATGACACTGCTCATCAATATACTGACAATGATGCCCACAAGTGGGTTAGCCACCGCAACCATAGGGGAAACATCTGTGGAGTCGACTTCATTTGGCTTTGTAATCCTAACAAGTCCCGTAAACCATTAAAGACCAGTTGGTGTGAAGCTGTTTTTGGAATATTAAGGCGCCAGCTTCGAAAAGCTTCGATGGCTGAAAATGATGCATTTGCCTTTTTGAAGGGCGACAACCATGGTGATTTTATTACTTCCTCTGCTTTCTGTGAAGCACTCCATCAGGTTAATTTAATTGGTCATCCATCCGGACTGGGTTTCCAGGAGACGAGGGATCTCTGGATCCAAGCAGACACAGATGCAAATGGTGTCCTTGATTATGAAGAATTCAAGAATATGATCTGGAGTTGTACAGGGTCAGACCAAAAGGAAAGCCTCACTGGGAGTAGAGAGGAGTCTGAGGAAGGCACCAAGGAAGCCCTTGGATTCAGGGTGAAGAATGCAGTTCTTTACCCAAGTGAGGCGGAGAGGGGAATATGGCCTGAAAATTACACACTTTCGGATCATGCTAGACTGACTGTTGTACTTTCACCAGAAAACATGTGGTGTTGTTCTCAGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_8 Exo_endo_phos

Protein Analysis

452

Amino Acids

51.92

Weight (kDa)

6.29

Isoelectric Point (pI)

43.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 68 - 296 1.1e-08 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013430)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02270 AT1G02270
fragaria_vesca FvH4_7g18080 FvH4_7g18080 FvH4_7g18080
malus_domestica MD01G1095200.v1.1 MD07G1163500.v1.1 MD07G1164200.v1.1
prunus_persica Prupe.2G203200_v2.0.a1
pyrus_communis pycom01g11740 pycom07g15930
rosa_chinensis RchiOBHm_Chr1g0361711
rosa_laevigata RLG00000027708
rosa_multiflora Rmu_sc0003426.1_g000004
rosa_rugosa Rorug01G0291000
rosa_samantha Rh1BG264200 Rh1DG294200
rosa_wichuraiana Rw1G026710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 909, 1202
AccB7I CCANNNNNTGG 1 cut(s) 101
AccI GTMKAC 2 cut(s) 251, 825
AccIII TCCGGA 1 cut(s) 1043
AciI CCGC 2 cut(s) 796, 1259
AclWI GGATC 5 cut(s) 600, 1072, 1077, 1085, 1305
AcsI RAATTY 4 cut(s) 293, 528, 606, 1121
AcuI CTGAAG 1 cut(s) 332
AcyI GRCGYC 1 cut(s) 910
AfaI GTAC 6 cut(s) 118, 308, 340, 707, 1147, 1320
AfiI CCNNNNNNNGG 5 cut(s) 95, 101, 783, 784, 1255
AflIII ACRYGT 2 cut(s) 472, 1335
AgsI TTSAA 7 cut(s) 63, 127, 346, 521, 734, 961, 1126
AjnI CCWGG 4 cut(s) 94, 100, 267, 1056
AjuI GAANNNNNNNTTGG 2 cut(s) 879, 911
AluBI AGCT 8 cut(s) 69, 195, 349, 466, 646, 890, 916, 926
AluI AGCT 8 cut(s) 69, 195, 349, 466, 646, 890, 916, 926
Alw26I GTCTC 4 cut(s) 18, 269, 444, 1055
AlwI GGATC 5 cut(s) 600, 1072, 1077, 1085, 1305
AlwNI CAGNNNCTG 1 cut(s) 101
Aor13HI TCCGGA 1 cut(s) 1043
AoxI GGCC 3 cut(s) 98, 624, 1274
ApoI RAATTY 4 cut(s) 293, 528, 606, 1121
Asp700I GAANNNNTTC 1 cut(s) 925
AspLEI GCGC 2 cut(s) 435, 912
AspS9I GGNCC 1 cut(s) 98
AsuHPI GGTGA 6 cut(s) 217, 335, 686, 989, 1240, 1317
AsuII TTCGAA 2 cut(s) 394, 919
BamHI GGATCC 1 cut(s) 1077
BanI GGYRCC 2 cut(s) 909, 1202
BccI CCATC 5 cut(s) 368, 668, 925, 1023, 1048
BceAI ACGGC 1 cut(s) 374
BcgI CGANNNNNNTGC 2 cut(s) 384, 418
BciT130I CCWGG 4 cut(s) 96, 102, 269, 1058
BcoDI GTCTC 4 cut(s) 18, 269, 444, 1055
BfaI CTAG 2 cut(s) 210, 1305
BfmI CTRYAG 1 cut(s) 334
BfoI RGCGCY 1 cut(s) 913
BlpI GCTNAGC 1 cut(s) 462
Bme1390I CCNGG 4 cut(s) 96, 102, 269, 1058
BmgT120I GGNCC 1 cut(s) 98
BmiI GGNNCC 5 cut(s) 87, 138, 911, 1079, 1204
BmrFI CCNGG 4 cut(s) 96, 102, 269, 1058
BmrI ACTGGG 2 cut(s) 1058, 1185
BmsI GCATC 4 cut(s) 316, 763, 934, 1084
BmuI ACTGGG 2 cut(s) 1058, 1185
BpmI CTGGAG 2 cut(s) 251, 1159
Bpu1102I GCTNAGC 1 cut(s) 462
Bpu14I TTCGAA 2 cut(s) 394, 919
BsaHI GRCGYC 1 cut(s) 910
BsaI GGTCTC 1 cut(s) 444
BsaJI CCNNGG 5 cut(s) 95, 367, 973, 1206, 1216
BsaWI WCCGGW 1 cut(s) 1043
BsaXI ACNNNNNCTCC 4 cut(s) 17, 47, 441, 471
Bsc4I CCNNNNNNNGG 5 cut(s) 95, 101, 783, 784, 1255
Bse1I ACTGG 6 cut(s) 570, 594, 683, 876, 1053, 1180
BseAI TCCGGA 1 cut(s) 1043
BseBI CCWGG 4 cut(s) 96, 102, 269, 1058
BseDI CCNNGG 5 cut(s) 95, 367, 973, 1206, 1216
BseGI GGATG 2 cut(s) 1036, 1040
BseLI CCNNNNNNNGG 5 cut(s) 95, 101, 783, 784, 1255
BseMII CTCAG 2 cut(s) 476, 1185
BseNI ACTGG 6 cut(s) 570, 594, 683, 876, 1053, 1180
BseRI GAGGAG 2 cut(s) 98, 1202
BshFI GGCC 3 cut(s) 100, 626, 1276
BshNI GGYRCC 2 cut(s) 909, 1202
BsiSI CCGG 1 cut(s) 1044
BslFI GGGAC 1 cut(s) 841
BslI CCNNNNNNNGG 5 cut(s) 95, 101, 783, 784, 1255
BsmAI GTCTC 4 cut(s) 18, 269, 444, 1055
BsmBI CGTCTC 1 cut(s) 1055
BsmFI GGGAC 1 cut(s) 841
BsmI GAATGC 1 cut(s) 1240
BsnI GGCC 3 cut(s) 100, 626, 1276
Bso31I GGTCTC 1 cut(s) 444
Bsp119I TTCGAA 2 cut(s) 394, 919
Bsp13I TCCGGA 1 cut(s) 1043
Bsp1407I TGTACA 2 cut(s) 705, 1145
Bsp143I GATC 6 cut(s) 592, 719, 1069, 1077, 1134, 1297
Bsp1720I GCTNAGC 1 cut(s) 462
Bsp19I CCATGG 1 cut(s) 973
BspACI CCGC 2 cut(s) 796, 1259
BspANI GGCC 3 cut(s) 100, 626, 1276
BspCNI CTCAG 2 cut(s) 475, 1186
BspEI TCCGGA 1 cut(s) 1043
BspHI TCATGA 1 cut(s) 562
BspLI GGNNCC 5 cut(s) 87, 138, 911, 1079, 1204
BspPI GGATC 5 cut(s) 600, 1072, 1077, 1085, 1305
BspT104I TTCGAA 2 cut(s) 394, 919
BspT107I GGYRCC 2 cut(s) 909, 1202
BspTNI GGTCTC 1 cut(s) 444
BsrGI TGTACA 2 cut(s) 705, 1145
BsrI ACTGG 6 cut(s) 570, 594, 683, 876, 1053, 1180
BssECI CCNNGG 5 cut(s) 95, 367, 973, 1206, 1216
BssMI GATC 6 cut(s) 592, 719, 1069, 1077, 1134, 1297
BssNI GRCGYC 1 cut(s) 910
BssT1I CCWWGG 3 cut(s) 973, 1206, 1216
Bst2UI CCWGG 4 cut(s) 96, 102, 269, 1058
Bst4CI ACNGT 5 cut(s) 14, 240, 368, 455, 1315
Bst6I CTCTTC 1 cut(s) 308
BstACI GRCGYC 1 cut(s) 910
BstAUI TGTACA 2 cut(s) 705, 1145
BstBI TTCGAA 2 cut(s) 394, 919
BstC8I GCNNGC 4 cut(s) 331, 351, 692, 914
BstDEI CTNAG 4 cut(s) 462, 716, 1194, 1350
BstDSI CCRYGG 2 cut(s) 367, 973
BstF5I GGATG 2 cut(s) 1036, 1040
BstH2I RGCGCY 1 cut(s) 913
BstHHI GCGC 2 cut(s) 435, 912
BstKTI GATC 6 cut(s) 595, 722, 1072, 1080, 1137, 1300
BstMAI GTCTC 4 cut(s) 18, 269, 444, 1055
BstMBI GATC 6 cut(s) 592, 719, 1069, 1077, 1134, 1297
BstMWI GCNNNNNNNGC 1 cut(s) 932
BstNI CCWGG 4 cut(s) 96, 102, 269, 1058
BstNSI RCATGY 2 cut(s) 476, 1339
BstSCI CCNGG 4 cut(s) 94, 100, 267, 1056
BstSFI CTRYAG 1 cut(s) 334
BstX2I RGATCY 2 cut(s) 1069, 1077
BstYI RGATCY 2 cut(s) 1069, 1077
BsuRI GGCC 3 cut(s) 100, 626, 1276
BtgI CCRYGG 2 cut(s) 367, 973
BtsCI GGATG 2 cut(s) 1036, 1040
BtsI GCAGTG 1 cut(s) 747
BtsIMutI CAGTG 2 cut(s) 747, 1173
Cac8I GCNNGC 4 cut(s) 331, 351, 692, 914
CaiI CAGNNNCTG 1 cut(s) 101
CciI TCATGA 1 cut(s) 562
CfoI GCGC 2 cut(s) 435, 912
Cfr13I GGNCC 1 cut(s) 98
Csp6I GTAC 6 cut(s) 117, 307, 339, 706, 1146, 1319
CviAII CATG 7 cut(s) 50, 473, 563, 701, 974, 1301, 1336
CviQI GTAC 6 cut(s) 117, 307, 339, 706, 1146, 1319
DdeI CTNAG 4 cut(s) 462, 716, 1194, 1350
DinI GGCGCC 1 cut(s) 911
DpnI GATC 6 cut(s) 594, 721, 1071, 1079, 1136, 1299
DpnII GATC 6 cut(s) 592, 719, 1069, 1077, 1134, 1297
Eam1104I CTCTTC 1 cut(s) 308
EarI CTCTTC 1 cut(s) 308
EciI GGCGGA 1 cut(s) 1274
Eco130I CCWWGG 3 cut(s) 973, 1206, 1216
Eco147I AGGCCT 1 cut(s) 626
Eco31I GGTCTC 1 cut(s) 444
Eco57I CTGAAG 1 cut(s) 332
EcoO109I RGGNCCY 1 cut(s) 98
EcoRI GAATTC 1 cut(s) 1121
EcoRII CCWGG 4 cut(s) 94, 100, 267, 1056
EcoT14I CCWWGG 3 cut(s) 973, 1206, 1216
EcoT22I ATGCAT 1 cut(s) 949
EgeI GGCGCC 1 cut(s) 911
EheI GGCGCC 1 cut(s) 911
ErhI CCWWGG 3 cut(s) 973, 1206, 1216
Esp3I CGTCTC 1 cut(s) 1055
FaeI CATG 7 cut(s) 53, 476, 566, 704, 977, 1304, 1339
FalI AAGNNNNNCTT 2 cut(s) 516, 548
FaqI GGGAC 1 cut(s) 841
FatI CATG 7 cut(s) 49, 472, 562, 700, 973, 1300, 1335
FauNDI CATATG 2 cut(s) 654, 742
FblI GTMKAC 2 cut(s) 251, 825
FokI GGATG 2 cut(s) 1023, 1027
FspBI CTAG 2 cut(s) 210, 1305
GlaI GCGC 2 cut(s) 434, 911
GsuI CTGGAG 2 cut(s) 251, 1159
HaeII RGCGCY 1 cut(s) 913
HaeIII GGCC 3 cut(s) 100, 626, 1276
HapII CCGG 1 cut(s) 1044
HhaI GCGC 2 cut(s) 435, 912
Hin1I GRCGYC 1 cut(s) 910
Hin1II CATG 7 cut(s) 53, 476, 566, 704, 977, 1304, 1339
Hin6I GCGC 2 cut(s) 433, 910
HinP1I GCGC 2 cut(s) 433, 910
HincII GTYRAC 1 cut(s) 826
HindII GTYRAC 1 cut(s) 826
HindIII AAGCTT 3 cut(s) 67, 347, 924
HpaII CCGG 1 cut(s) 1044
HphI GGTGA 6 cut(s) 217, 335, 686, 989, 1240, 1317
Hpy166II GTNNAC 6 cut(s) 252, 541, 601, 706, 826, 863
Hpy188I TCNGA 4 cut(s) 219, 1156, 1195, 1297
Hpy188III TCNNGA 9 cut(s) 63, 179, 425, 505, 563, 1044, 1075, 1126, 1138
Hpy8I GTNNAC 6 cut(s) 252, 541, 601, 706, 826, 863
HpyAV CCTTC 5 cut(s) 352, 502, 527, 955, 1193
HpyCH4III ACNGT 5 cut(s) 14, 240, 368, 455, 1315
HpyCH4V TGCA 4 cut(s) 613, 947, 1097, 1238
HpyF10VI GCNNNNNNNGC 1 cut(s) 932
HpyF3I CTNAG 4 cut(s) 462, 716, 1194, 1350
Hsp92I GRCGYC 1 cut(s) 910
Hsp92II CATG 7 cut(s) 53, 476, 566, 704, 977, 1304, 1339
HspAI GCGC 2 cut(s) 433, 910
KasI GGCGCC 1 cut(s) 909
Kpn2I TCCGGA 1 cut(s) 1043
Kzo9I GATC 6 cut(s) 592, 719, 1069, 1077, 1134, 1297
LmnI GCTCC 3 cut(s) 85, 142, 493
LweI GCATC 4 cut(s) 316, 763, 934, 1084
MaeI CTAG 2 cut(s) 210, 1305
MaeIII GTNAC 3 cut(s) 205, 234, 674
MalI GATC 6 cut(s) 594, 721, 1071, 1079, 1136, 1299
MboI GATC 6 cut(s) 592, 719, 1069, 1077, 1134, 1297
MboII GAAGA 5 cut(s) 232, 302, 325, 1130, 1243
MflI RGATCY 2 cut(s) 1069, 1077
Mly113I GGCGCC 1 cut(s) 910
MlyI GAGTC 5 cut(s) 26, 147, 193, 831, 1199
MmeI TCCRAC 1 cut(s) 262
Mph1103I ATGCAT 1 cut(s) 949
MroI TCCGGA 1 cut(s) 1043
MroXI GAANNNNTTC 1 cut(s) 925
MseI TTAA 6 cut(s) 438, 482, 869, 905, 1023, 1028
MspI CCGG 1 cut(s) 1044
MspR9I CCNGG 4 cut(s) 96, 102, 269, 1058
Mva1269I GAATGC 1 cut(s) 1240
MvaI CCWGG 4 cut(s) 96, 102, 269, 1058
MwoI GCNNNNNNNGC 1 cut(s) 932
NarI GGCGCC 1 cut(s) 910
NcoI CCATGG 1 cut(s) 973
NdeI CATATG 2 cut(s) 654, 742
NdeII GATC 6 cut(s) 592, 719, 1069, 1077, 1134, 1297
NlaIII CATG 7 cut(s) 53, 476, 566, 704, 977, 1304, 1339
NlaIV GGNNCC 5 cut(s) 87, 138, 911, 1079, 1204
NmuCI GTSAC 2 cut(s) 205, 674
NsiI ATGCAT 1 cut(s) 949
NspI RCATGY 2 cut(s) 476, 1339
NspV TTCGAA 2 cut(s) 394, 919
PagI TCATGA 1 cut(s) 562
PceI AGGCCT 1 cut(s) 626
PciI ACATGT 2 cut(s) 472, 1335
PctI GAATGC 1 cut(s) 1240
PdmI GAANNNNTTC 1 cut(s) 925
PfeI GAWTC 6 cut(s) 166, 214, 245, 566, 734, 1221
PflMI CCANNNNNTGG 1 cut(s) 101
PfoI TCCNGGA 2 cut(s) 267, 1056
PleI GAGTC 5 cut(s) 26, 147, 192, 830, 1198
PluTI GGCGCC 1 cut(s) 913
PpsI GAGTC 5 cut(s) 26, 147, 192, 830, 1198
PscI ACATGT 2 cut(s) 472, 1335
Psp6I CCWGG 4 cut(s) 94, 100, 267, 1056
PspGI CCWGG 4 cut(s) 94, 100, 267, 1056
PspN4I GGNNCC 5 cut(s) 87, 138, 911, 1079, 1204
PspPI GGNCC 1 cut(s) 98
PstNI CAGNNNCTG 1 cut(s) 101
PsuI RGATCY 2 cut(s) 1069, 1077
RsaI GTAC 6 cut(s) 118, 308, 340, 707, 1147, 1320
RsaNI GTAC 6 cut(s) 117, 307, 339, 706, 1146, 1319
SalI GTCGAC 1 cut(s) 824
SaqAI TTAA 6 cut(s) 438, 482, 869, 905, 1023, 1028
Sau3AI GATC 6 cut(s) 592, 719, 1069, 1077, 1134, 1297
Sau96I GGNCC 1 cut(s) 98
SchI GAGTC 5 cut(s) 26, 147, 193, 831, 1199
ScrFI CCNGG 4 cut(s) 96, 102, 269, 1058
SfaNI GCATC 4 cut(s) 316, 763, 934, 1084
SfcI CTRYAG 1 cut(s) 334
SfoI GGCGCC 1 cut(s) 911
SfuI TTCGAA 2 cut(s) 394, 919
SseBI AGGCCT 1 cut(s) 626
SsiI CCGC 2 cut(s) 796, 1259
SspDI GGCGCC 1 cut(s) 909
SspI AATATT 2 cut(s) 130, 903
SspMI CTAG 2 cut(s) 210, 1305
StuI AGGCCT 1 cut(s) 626
StyD4I CCNGG 4 cut(s) 94, 100, 267, 1056
StyI CCWWGG 3 cut(s) 973, 1206, 1216
TaaI ACNGT 5 cut(s) 14, 240, 368, 455, 1315
TaqI TCGA 6 cut(s) 156, 394, 424, 825, 919, 929
TatI WGTACW 4 cut(s) 116, 705, 1145, 1318
TfiI GAWTC 6 cut(s) 166, 214, 245, 566, 734, 1221
Tru1I TTAA 6 cut(s) 438, 482, 869, 905, 1023, 1028
Tru9I TTAA 6 cut(s) 438, 482, 869, 905, 1023, 1028
TscAI CASTG 2 cut(s) 754, 1180
TseFI GTSAC 2 cut(s) 205, 674
Tsp45I GTSAC 2 cut(s) 205, 674
TspDTI ATGAA 5 cut(s) 258, 303, 651, 820, 1131
TspRI CASTG 2 cut(s) 754, 1180
Van91I CCANNNNNTGG 1 cut(s) 101
XapI RAATTY 4 cut(s) 293, 528, 606, 1121
XceI RCATGY 2 cut(s) 476, 1339
XcmI CCANNNNNNNNNTGG 1 cut(s) 1335
XmiI GTMKAC 2 cut(s) 251, 825
XmnI GAANNNNTTC 1 cut(s) 925
XspI CTAG 2 cut(s) 210, 1305
Zsp2I ATGCAT 1 cut(s) 949
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.