pycom01g11740

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0013430
Physical Location & Seq
Reverse (-)
12792706 .. 12796071
3366 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g11740.8

Sequence Viewer

Length: 1464 bp
ATGTTGGTATCTAAGAAAATGGGTACTCGGGAAATCGGTTTTTGGATATTGGGTAATGAAGATTTTGAGAATTTGCAGGGTTTAGGTGGAGGTTCTAATGTGGCAAAGGTCAATCGCAGAGAAATTGAGAGCCTTTCAAGGAGCTATAGCGGCAGCTGCACTTCGTTTCCGTCGATATCAGAGCCAAAGCCAGAGACTTTGGCAGGGCCTTGCTTAGTTTCGTGCACGACTTTCAATATTTTGGCTCCAATCTATAAACGACTCGATCAACAGAATCAAAGCCTTCGAGAGAGCAACTACAGGGAGTTTTGGGTGGCCAGGAACCAGAGGATTTTGGATTGGTTGCTCTATGAATCATCCGCCATTATTTGTCTGCAGGAGTTCTGGGTTGGAAATGAAGAATTTGTGAATATGTACCTGGACAGGCTTGGTGATGCAGGCTATACTACCTTCAAGCTTGCCCGAACTAACAACCGGGGTGACGGTTTGCTGACTGCTGTGCGGAGGGATTGCTTTAGTGTTCTAAACTATCGAGAGTTGCATTTTAATGATTTTGGAGATCGTGTTGCTCAGCTGTTACATGTTCAGTTAGCTTCCCCATTTTCACAAAACCAGAGAGGAAACGTTCAGCAAGAAATGCTAATTGTGAATACTCACTTGTTATTTCCTCACGATTCCAGTCTCTCTATAGTGCGATTGCATCAGGTTTACAAAATTTTGCAATTTGTGGAGTCATATCAGAATGAAAACAAGCTAAACCCGATGCCCATTGTACTCTGTGGTGACTGGAATGGAAGTAAACGTGGCCATGTGTACAAATTCCTTAGATCGCAGGGGTTTGAATCAACATATGATGCTGCTCATCAATATACTGATGCGGATGCTCACAAGTGGGTTAGCCACCGTAATCATAGGGGAAACATCTGCGGTGTTGACTTCATTTGGCTTTGTAATCCCAATAAGTCACGAAAACCACTAAAGACTAGTTGGTGCGAAGCTGTTTTTGGAATATTACGGCGCCAGCTCCGAAAAGCTTCGATGGCTGAAAATGATGCATTTACCTTTCTAAAGGGTGATAGTCATGGTGATTTTATTACGACCTCAGCTTTCTGTGAAGGACTACATCAGGTTAACTTAATTGGTCATCCTACGGGACTAGGTTTCCAGGAGACGAGGGATCTCTGGATCCAAGCAGATGCCGACGCAAATGGTTTCTTTGATTACGAAGAATTTAAGAGTAGAATCTGGAGTTCGACAGCGTCAGAGGAAAAGGAAAGCCTTAACGCTAGTAGAGAGGAGTCCAAACGAGGCACGCAGGGCGCCCTTGGATTCAATGTGAAGAATGCAGTTCTATACCCCCGTGAGGCGGAGAAGGGAATATGGCCCGATGATTACACTCTCTCTGACCATGCGGGACTATCGGTTGTACTTTCACCAGAAAGGATGTGGTGTTGCCAGTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_8 Exo_endo_phos

Protein Analysis

488

Amino Acids

55.54

Weight (kDa)

6.41

Isoelectric Point (pI)

43.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013430)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02270 AT1G02270
fragaria_vesca FvH4_7g18080 FvH4_7g18080 FvH4_7g18080
malus_domestica MD01G1095200.v1.1 MD07G1163500.v1.1 MD07G1164200.v1.1
prunus_persica Prupe.2G203200_v2.0.a1
pyrus_communis pycom01g11740 pycom07g15930
rosa_chinensis RchiOBHm_Chr1g0361711
rosa_laevigata RLG00000027708
rosa_multiflora Rmu_sc0003426.1_g000004
rosa_rugosa Rorug01G0291000
rosa_samantha Rh1BG264200 Rh1DG294200
rosa_wichuraiana Rw1G026710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1017, 1319
AciI CCGC 7 cut(s) 150, 360, 502, 878, 927, 1367, 1412
AclI AACGTT 1 cut(s) 624
AclWI GGATC 3 cut(s) 1180, 1185, 1193
AcoI YGGCCR 2 cut(s) 315, 805
AcsI RAATTY 5 cut(s) 70, 401, 714, 818, 1229
AcyI GRCGYC 2 cut(s) 1018, 1320
AfaI GTAC 5 cut(s) 25, 416, 774, 815, 1428
AfiI CCNNNNNNNGG 2 cut(s) 1363, 1366
AflIII ACRYGT 1 cut(s) 580
AgsI TTSAA 5 cut(s) 138, 235, 454, 842, 1333
AhlI ACTAGT 1 cut(s) 983
AjnI CCWGG 3 cut(s) 317, 417, 1164
AjuI GAANNNNNNNTTGG 2 cut(s) 987, 1019
Alw21I GWGCWC 1 cut(s) 227
Alw26I GTCTC 3 cut(s) 188, 686, 1163
Alw44I GTGCAC 1 cut(s) 223
AlwI GGATC 3 cut(s) 1180, 1185, 1193
Ama87I CYCGRG 1 cut(s) 27
AoxI GGCC 4 cut(s) 206, 315, 805, 1382
ApaLI GTGCAC 1 cut(s) 223
ApeKI GCWGC 3 cut(s) 153, 156, 857
ApoI RAATTY 5 cut(s) 70, 401, 714, 818, 1229
Asp700I GAANNNNTTC 1 cut(s) 1033
AspLEI GCGC 2 cut(s) 1020, 1322
AspS9I GGNCC 2 cut(s) 206, 1383
AsuC2I CCSGG 1 cut(s) 476
AsuHPI GGTGA 6 cut(s) 443, 491, 794, 1085, 1097, 1425
AvaI CYCGRG 1 cut(s) 27
BaeGI GKGCMC 1 cut(s) 227
BalI TGGCCA 2 cut(s) 317, 807
BamHI GGATCC 1 cut(s) 1185
BanI GGYRCC 2 cut(s) 1017, 1319
Bbv12I GWGCWC 1 cut(s) 227
BbvCI CCTCAGC 1 cut(s) 1102
BbvI GCAGC 3 cut(s) 143, 165, 844
BccI CCATC 1 cut(s) 1033
BceAI ACGGC 1 cut(s) 1031
BciT130I CCWGG 3 cut(s) 319, 419, 1166
BcnI CCSGG 1 cut(s) 476
BcoDI GTCTC 3 cut(s) 188, 686, 1163
BcuI ACTAGT 1 cut(s) 983
BfaI CTAG 3 cut(s) 984, 1157, 1287
BfmI CTRYAG 4 cut(s) 145, 298, 374, 687
BfoI RGCGCY 2 cut(s) 1021, 1323
BisI GCNGC 4 cut(s) 151, 154, 157, 858
BlpI GCTNAGC 1 cut(s) 570
BlsI GCNGC 4 cut(s) 152, 155, 158, 859
Bme1390I CCNGG 4 cut(s) 319, 419, 476, 1166
BmeT110I CYCGRG 1 cut(s) 27
BmgT120I GGNCC 2 cut(s) 206, 1383
BmiI GGNNCC 5 cut(s) 246, 323, 1019, 1187, 1321
BmrFI CCNGG 4 cut(s) 319, 419, 476, 1166
BmsI GCATC 8 cut(s) 424, 709, 753, 844, 865, 871, 1042, 1186
BpmI CTGGAG 1 cut(s) 1267
Bpu10I CCTNAGC 1 cut(s) 1102
Bpu1102I GCTNAGC 1 cut(s) 570
BpuMI CCSGG 1 cut(s) 476
BsaHI GRCGYC 2 cut(s) 1018, 1320
BsaJI CCNNGG 2 cut(s) 475, 1324
BsaXI ACNNNNNCTCC 2 cut(s) 549, 579
Bsc4I CCNNNNNNNGG 2 cut(s) 1363, 1366
Bse1I ACTGG 3 cut(s) 678, 791, 1456
BseBI CCWGG 3 cut(s) 319, 419, 1166
BseDI CCNNGG 2 cut(s) 475, 1324
BseGI GGATG 4 cut(s) 356, 886, 1144, 1449
BseLI CCNNNNNNNGG 2 cut(s) 1363, 1366
BseMII CTCAG 2 cut(s) 584, 1116
BseNI ACTGG 3 cut(s) 678, 791, 1456
BseRI GAGGAG 1 cut(s) 1310
BseSI GKGCMC 1 cut(s) 227
BseXI GCAGC 3 cut(s) 143, 165, 844
BsgI GTGCAG 1 cut(s) 142
BshFI GGCC 4 cut(s) 208, 317, 807, 1384
BshNI GGYRCC 2 cut(s) 1017, 1319
BsiHKAI GWGCWC 1 cut(s) 227
BsiHKCI CYCGRG 1 cut(s) 27
BsiSI CCGG 1 cut(s) 475
BslFI GGGAC 2 cut(s) 1167, 1428
BslI CCNNNNNNNGG 2 cut(s) 1363, 1366
BsmAI GTCTC 3 cut(s) 188, 686, 1163
BsmBI CGTCTC 1 cut(s) 1163
BsmFI GGGAC 2 cut(s) 1167, 1428
BsmI GAATGC 1 cut(s) 1348
BsnI GGCC 4 cut(s) 208, 317, 807, 1384
BsoBI CYCGRG 1 cut(s) 27
Bsp1286I GDGCHC 1 cut(s) 227
Bsp1407I TGTACA 1 cut(s) 813
Bsp143I GATC 5 cut(s) 265, 559, 827, 1177, 1185
Bsp1720I GCTNAGC 1 cut(s) 570
BspACI CCGC 7 cut(s) 150, 360, 502, 878, 927, 1367, 1412
BspANI GGCC 4 cut(s) 208, 317, 807, 1384
BspCNI CTCAG 2 cut(s) 583, 1115
BspLI GGNNCC 5 cut(s) 246, 323, 1019, 1187, 1321
BspMAI CTGCAG 1 cut(s) 378
BspPI GGATC 3 cut(s) 1180, 1185, 1193
BspT107I GGYRCC 2 cut(s) 1017, 1319
BsrGI TGTACA 1 cut(s) 813
BsrI ACTGG 3 cut(s) 678, 791, 1456
BssECI CCNNGG 2 cut(s) 475, 1324
BssMI GATC 5 cut(s) 265, 559, 827, 1177, 1185
BssNI GRCGYC 2 cut(s) 1018, 1320
BssT1I CCWWGG 1 cut(s) 1324
Bst2UI CCWGG 3 cut(s) 319, 419, 1166
Bst4CI ACNGT 2 cut(s) 485, 905
BstACI GRCGYC 2 cut(s) 1018, 1320
BstAPI GCANNNNNTGC 1 cut(s) 637
BstAUI TGTACA 1 cut(s) 813
BstC8I GCNNGC 4 cut(s) 439, 459, 1022, 1313
BstDEI CTNAG 5 cut(s) 12, 214, 570, 824, 1102
BstF5I GGATG 4 cut(s) 356, 886, 1144, 1449
BstH2I RGCGCY 2 cut(s) 1021, 1323
BstHHI GCGC 2 cut(s) 1020, 1322
BstKTI GATC 5 cut(s) 268, 562, 830, 1180, 1188
BstMAI GTCTC 3 cut(s) 188, 686, 1163
BstMBI GATC 5 cut(s) 265, 559, 827, 1177, 1185
BstMWI GCNNNNNNNGC 5 cut(s) 150, 156, 637, 1040, 1317
BstNI CCWGG 3 cut(s) 319, 419, 1166
BstNSI RCATGY 1 cut(s) 584
BstSCI CCNGG 4 cut(s) 317, 417, 474, 1164
BstSFI CTRYAG 4 cut(s) 145, 298, 374, 687
BstSLI GKGCMC 1 cut(s) 227
BstV1I GCAGC 3 cut(s) 143, 165, 844
BstX2I RGATCY 2 cut(s) 1177, 1185
BstYI RGATCY 2 cut(s) 1177, 1185
BsuRI GGCC 4 cut(s) 208, 317, 807, 1384
BtsCI GGATG 4 cut(s) 356, 886, 1144, 1449
Cac8I GCNNGC 4 cut(s) 439, 459, 1022, 1313
CfoI GCGC 2 cut(s) 1020, 1322
Cfr13I GGNCC 2 cut(s) 206, 1383
CseI GACGC 2 cut(s) 1211, 1248
Csp6I GTAC 5 cut(s) 24, 415, 773, 814, 1427
CviAII CATG 4 cut(s) 581, 809, 1082, 1409
CviQI GTAC 5 cut(s) 24, 415, 773, 814, 1427
DdeI CTNAG 5 cut(s) 12, 214, 570, 824, 1102
DinI GGCGCC 2 cut(s) 1019, 1321
DpnI GATC 5 cut(s) 267, 561, 829, 1179, 1187
DpnII GATC 5 cut(s) 265, 559, 827, 1177, 1185
EaeI YGGCCR 2 cut(s) 315, 805
EciI GGCGGA 2 cut(s) 349, 1382
Eco130I CCWWGG 1 cut(s) 1324
Eco32I GATATC 1 cut(s) 177
Eco88I CYCGRG 1 cut(s) 27
EcoO109I RGGNCCY 1 cut(s) 206
EcoRII CCWGG 3 cut(s) 317, 417, 1164
EcoRV GATATC 1 cut(s) 177
EcoT14I CCWWGG 1 cut(s) 1324
EcoT22I ATGCAT 1 cut(s) 1057
EgeI GGCGCC 2 cut(s) 1019, 1321
EheI GGCGCC 2 cut(s) 1019, 1321
ErhI CCWWGG 1 cut(s) 1324
Esp3I CGTCTC 1 cut(s) 1163
FaeI CATG 4 cut(s) 584, 812, 1085, 1412
FaqI GGGAC 2 cut(s) 1167, 1428
FatI CATG 4 cut(s) 580, 808, 1081, 1408
FauI CCCGC 1 cut(s) 1405
FauNDI CATATG 1 cut(s) 850
Fnu4HI GCNGC 4 cut(s) 151, 154, 157, 858
FokI GGATG 4 cut(s) 343, 893, 1131, 1456
Fsp4HI GCNGC 4 cut(s) 151, 154, 157, 858
FspBI CTAG 3 cut(s) 984, 1157, 1287
GlaI GCGC 2 cut(s) 1019, 1321
GluI GCNGC 4 cut(s) 151, 154, 157, 858
GsuI CTGGAG 1 cut(s) 1267
HaeII RGCGCY 2 cut(s) 1021, 1323
HaeIII GGCC 4 cut(s) 208, 317, 807, 1384
HapII CCGG 1 cut(s) 475
HgaI GACGC 2 cut(s) 1211, 1248
HhaI GCGC 2 cut(s) 1020, 1322
Hin1I GRCGYC 2 cut(s) 1018, 1320
Hin1II CATG 4 cut(s) 584, 812, 1085, 1412
Hin6I GCGC 2 cut(s) 1018, 1320
HinP1I GCGC 2 cut(s) 1018, 1320
HincII GTYRAC 2 cut(s) 934, 1132
HindII GTYRAC 2 cut(s) 934, 1132
HindIII AAGCTT 2 cut(s) 455, 1032
HinfI GANTC 9 cut(s) 261, 274, 353, 674, 731, 842, 1242, 1298, 1329
HpaI GTTAAC 1 cut(s) 1132
HpaII CCGG 1 cut(s) 475
HphI GGTGA 6 cut(s) 443, 491, 794, 1085, 1097, 1425
Hpy166II GTNNAC 6 cut(s) 225, 709, 800, 814, 934, 1132
Hpy188I TCNGA 5 cut(s) 181, 741, 1028, 1264, 1405
Hpy188III TCNNGA 7 cut(s) 29, 287, 533, 671, 966, 1183, 1246
Hpy8I GTNNAC 6 cut(s) 225, 709, 800, 814, 934, 1132
Hpy99I CGWCG 2 cut(s) 175, 1205
HpyAV CCTTC 4 cut(s) 293, 460, 1109, 1366
HpyCH4III ACNGT 2 cut(s) 485, 905
HpyCH4IV ACGT 2 cut(s) 624, 802
HpyF10VI GCNNNNNNNGC 5 cut(s) 150, 156, 637, 1040, 1317
HpyF3I CTNAG 5 cut(s) 12, 214, 570, 824, 1102
HpySE526I ACGT 2 cut(s) 624, 802
Hsp92I GRCGYC 2 cut(s) 1018, 1320
Hsp92II CATG 4 cut(s) 584, 812, 1085, 1412
HspAI GCGC 2 cut(s) 1018, 1320
KasI GGCGCC 2 cut(s) 1017, 1319
KspAI GTTAAC 1 cut(s) 1132
Kzo9I GATC 5 cut(s) 265, 559, 827, 1177, 1185
LmnI GCTCC 3 cut(s) 141, 250, 1029
Lsp1109I GCAGC 3 cut(s) 143, 165, 844
LweI GCATC 8 cut(s) 424, 709, 753, 844, 865, 871, 1042, 1186
MaeI CTAG 3 cut(s) 984, 1157, 1287
MaeII ACGT 2 cut(s) 624, 802
MaeIII GTNAC 4 cut(s) 479, 576, 782, 963
MalI GATC 5 cut(s) 267, 561, 829, 1179, 1187
MboI GATC 5 cut(s) 265, 559, 827, 1177, 1185
MboII GAAGA 4 cut(s) 71, 410, 1238, 1351
MflI RGATCY 2 cut(s) 1177, 1185
MhlI GDGCHC 1 cut(s) 227
MlsI TGGCCA 2 cut(s) 317, 807
MluCI AATT 9 cut(s) 70, 123, 401, 642, 714, 722, 818, 1137, 1229
MluNI TGGCCA 2 cut(s) 317, 807
Mly113I GGCGCC 2 cut(s) 1018, 1320
MlyI GAGTC 3 cut(s) 255, 740, 1307
MmeI TCCRAC 1 cut(s) 370
Mox20I TGGCCA 2 cut(s) 317, 807
Mph1103I ATGCAT 1 cut(s) 1057
MroXI GAANNNNTTC 1 cut(s) 1033
MscI TGGCCA 2 cut(s) 317, 807
MseI TTAA 5 cut(s) 546, 1131, 1136, 1233, 1281
MslI CAYNNNNRTG 1 cut(s) 546
Msp20I TGGCCA 2 cut(s) 317, 807
MspA1I CMGCKG 2 cut(s) 156, 574
MspI CCGG 1 cut(s) 475
MspR9I CCNGG 4 cut(s) 319, 419, 476, 1166
Mva1269I GAATGC 1 cut(s) 1348
MvaI CCWGG 3 cut(s) 319, 419, 1166
MwoI GCNNNNNNNGC 5 cut(s) 150, 156, 637, 1040, 1317
NarI GGCGCC 2 cut(s) 1018, 1320
NciI CCSGG 1 cut(s) 476
NdeI CATATG 1 cut(s) 850
NdeII GATC 5 cut(s) 265, 559, 827, 1177, 1185
NlaIII CATG 4 cut(s) 584, 812, 1085, 1412
NlaIV GGNNCC 5 cut(s) 246, 323, 1019, 1187, 1321
NmuCI GTSAC 3 cut(s) 479, 782, 963
NsiI ATGCAT 1 cut(s) 1057
NspI RCATGY 1 cut(s) 584
PciI ACATGT 1 cut(s) 580
PcsI WCGNNNNNNNCGW 1 cut(s) 170
PctI GAATGC 1 cut(s) 1348
PdmI GAANNNNTTC 1 cut(s) 1033
PfeI GAWTC 6 cut(s) 274, 353, 674, 842, 1242, 1329
PflFI GACNNNGTC 1 cut(s) 1258
PfoI TCCNGGA 1 cut(s) 1164
PkrI GCNGC 4 cut(s) 152, 155, 158, 859
PleI GAGTC 3 cut(s) 255, 739, 1306
PluTI GGCGCC 2 cut(s) 1021, 1323
PpsI GAGTC 3 cut(s) 255, 739, 1306
PscI ACATGT 1 cut(s) 580
Psp1406I AACGTT 1 cut(s) 624
Psp6I CCWGG 3 cut(s) 317, 417, 1164
PspGI CCWGG 3 cut(s) 317, 417, 1164
PspN4I GGNNCC 5 cut(s) 246, 323, 1019, 1187, 1321
PspPI GGNCC 2 cut(s) 206, 1383
PstI CTGCAG 1 cut(s) 378
PsuI RGATCY 2 cut(s) 1177, 1185
PsyI GACNNNGTC 1 cut(s) 1258
PvuII CAGCTG 2 cut(s) 156, 574
RsaI GTAC 5 cut(s) 25, 416, 774, 815, 1428
RsaNI GTAC 5 cut(s) 24, 415, 773, 814, 1427
RseI CAYNNNNRTG 1 cut(s) 546
SaqAI TTAA 5 cut(s) 546, 1131, 1136, 1233, 1281
SatI GCNGC 4 cut(s) 151, 154, 157, 858
Sau3AI GATC 5 cut(s) 265, 559, 827, 1177, 1185
Sau96I GGNCC 2 cut(s) 206, 1383
SchI GAGTC 3 cut(s) 255, 740, 1307
ScrFI CCNGG 4 cut(s) 319, 419, 476, 1166
SduI GDGCHC 1 cut(s) 227
SfaNI GCATC 8 cut(s) 424, 709, 753, 844, 865, 871, 1042, 1186
SfcI CTRYAG 4 cut(s) 145, 298, 374, 687
SfoI GGCGCC 2 cut(s) 1019, 1321
SmiMI CAYNNNNRTG 1 cut(s) 546
SpeI ACTAGT 1 cut(s) 983
Sse9I AATT 9 cut(s) 70, 123, 401, 642, 714, 722, 818, 1137, 1229
SsiI CCGC 7 cut(s) 150, 360, 502, 878, 927, 1367, 1412
SspDI GGCGCC 2 cut(s) 1017, 1319
SspI AATATT 2 cut(s) 238, 1011
SspMI CTAG 3 cut(s) 984, 1157, 1287
StyD4I CCNGG 4 cut(s) 317, 417, 474, 1164
StyI CCWWGG 1 cut(s) 1324
TaaI ACNGT 2 cut(s) 485, 905
TaiI ACGT 2 cut(s) 627, 805
TaqI TCGA 6 cut(s) 173, 264, 286, 532, 1037, 1253
TasI AATT 9 cut(s) 70, 123, 401, 642, 714, 722, 818, 1137, 1229
TatI WGTACW 3 cut(s) 772, 813, 1426
TauI GCSGC 1 cut(s) 153
TfiI GAWTC 6 cut(s) 274, 353, 674, 842, 1242, 1329
Tru1I TTAA 5 cut(s) 546, 1131, 1136, 1233, 1281
Tru9I TTAA 5 cut(s) 546, 1131, 1136, 1233, 1281
TseFI GTSAC 3 cut(s) 479, 782, 963
TseI GCWGC 3 cut(s) 153, 156, 857
Tsp45I GTSAC 3 cut(s) 479, 782, 963
TspDTI ATGAA 5 cut(s) 72, 366, 411, 759, 928
TspGWI ACGGA 1 cut(s) 159
Tth111I GACNNNGTC 1 cut(s) 1258
VneI GTGCAC 1 cut(s) 223
XapI RAATTY 5 cut(s) 70, 401, 714, 818, 1229
XceI RCATGY 1 cut(s) 584
XcmI CCANNNNNNNNNTGG 1 cut(s) 1443
XmnI GAANNNNTTC 1 cut(s) 1033
XspI CTAG 3 cut(s) 984, 1157, 1287
Zsp2I ATGCAT 1 cut(s) 1057
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.