AT1G03475

coproporphyrinogen oxidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0011336
Physical Location & Seq
Reverse (-)
868853 .. 871308
2456 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G03475.1

Sequence Viewer

Length: 1161 bp
ATGGCTTCTCACTCGTCGACTCTCCTCTCTTCTCCTACTTTCGCTCCTTTCTCCTCTCATCGTCTTCATTATTCTCCCAATCCCTCTACTCTCAGATTCTCCCGTCCAATCAGAAATAAACCTAATCTCGCCTTGCGATGTTCAGTCTCAATTGAGAAAGAAGTTCCCGAAACTGAACGACCCTTTACTTTCCTTAGGGATTCTGATGACGTCACTCCATCTTCTTCTTCTTCTTCCGTCAGGGCTCGTTTCGAGACTATGATTAGGGCTGCTCAAGACAGTGTTTGTGATGCAATTGAAGCTATAGAAGGCGGTCCTAAGTTTAAAGAAGATGTTTGGTCTCGACCTGGTGGTGGCGGTGGAATCAGTCGTGTGTTGCAGGACGGGAATGTCTTTGAGAAAGCTGGTGTTAATGTCTCTGTGGTTTATGGTGTTATGCCTCCTGAAGCTTATAGAGCTGCAAAGGGCTCAGCTTCTGATCAGAAACCTGGTCCTGTTCCGTTCTTCGCTGCTGGAGTCAGCTCGGTTTTGCATCCCAAGAACCCTTTTGCCCCAACCCTGCATTTCAATTATCGCTATTTCGAGACAGATGCTCCAAAGGATGTTCCTGGAGCTCCAAGGCAATGGTGGTTTGGTGGTGGCACTGATTTCACTCCTGCTTACATCTTTGAAGAAGATGTCAAGCATTTCCATTCGATTCAAAAGCAAGCCTGTGACAAATTTGACCCTTCCTTCTATCCCCGATTCAAGAAGTGGTGTGATGACTACTTTTACATCAAGCACCGTGATGAGAGACGAGGACTTGGAGGGATATTTTTTGATGATCTTAATGACTATGATCAGGAAATGCTTCTGTCATTTGCCACTGAATGCGCAAACTCAGTGGTGCCGGCTTATATACCTATAGTAGAGAAAAGGAAAGACATGGAATTTACAGAGCAGCACAAGGCATGGCAACAGTTGCGACGAGGGCGATATGTCGAATTCAACTTGGTATATGATCGGGGAACGACATTTGGTCTGAAGACAGGAGGACGAATAGAGAGCATTCTCGTCTCTCTTCCGCTTTCAGCAAGATGGGAATATGACCATAAACCGGAAGAGGGGACCGAAGAGTGGAAGCTATTGGATGCTTGCATCAACCCGAAGGAGTGGATCTAG

Protein Analysis

386

Amino Acids

43.8

Weight (kDa)

6.24

Isoelectric Point (pI)

63.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Coprogen_oxidas PF01218 84 - 385 1.2e-136 Coproporphyrinogen III oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011336)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03475 AT4G03205 AT4G03205
fragaria_vesca FvH4_6g39170
malus_domestica MD09G1144600.v1.1 MD17G1131200.v1.1
prunus_persica Prupe.3G183400_v2.0.a1
pyrus_communis pycom09g06400 pycom17g12400
rosa_chinensis RchiOBHm_Chr2g0153001
rosa_laevigata RLG00000020666
rosa_multiflora Rmu_sc0000218.1_g000004 Rmu_sc0000218.1_g000005
rosa_roxburghii Rroxscaffold_2G00095310
rosa_rugosa Rorug02G0436700
rosa_samantha Rh2AG498700 Rh2BG510000 Rh2CG485000 Rh2DG521600
rosa_wichuraiana Rw2G040930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 389
AatII GACGTC 1 cut(s) 213
Acc16I TGCGCA 1 cut(s) 874
AccB1I GGYRCC 1 cut(s) 886
AccI GTMKAC 1 cut(s) 17
AciI CCGC 3 cut(s) 312, 357, 1064
AcsI RAATTY 3 cut(s) 719, 929, 983
AcuI CTGAAG 2 cut(s) 465, 1043
AcyI GRCGYC 1 cut(s) 210
AfiI CCNNNNNNNGG 4 cut(s) 353, 1096, 1103, 1116
AgsI TTSAA 6 cut(s) 299, 568, 671, 701, 748, 988
AhdI GACNNNNNGTC 1 cut(s) 1017
AjnI CCWGG 3 cut(s) 346, 487, 607
AluBI AGCT 8 cut(s) 302, 404, 449, 458, 473, 522, 614, 1123
AluI AGCT 8 cut(s) 302, 404, 449, 458, 473, 522, 614, 1123
Alw21I GWGCWC 1 cut(s) 616
Alw26I GTCTC 7 cut(s) 151, 248, 345, 421, 578, 787, 1060
AlwNI CAGNNNCTG 1 cut(s) 476
ApeKI GCWGC 4 cut(s) 269, 458, 509, 940
ApoI RAATTY 3 cut(s) 719, 929, 983
Asp700I GAANNNNTTC 1 cut(s) 849
AspLEI GCGC 1 cut(s) 875
AspS9I GGNCC 3 cut(s) 314, 491, 1107
AvaII GGWCC 3 cut(s) 314, 491, 1107
AxyI CCTNAGG 1 cut(s) 194
BanI GGYRCC 1 cut(s) 886
BanII GRGCYC 3 cut(s) 247, 470, 616
BbsI GAAGAC 2 cut(s) 56, 1031
Bbv12I GWGCWC 1 cut(s) 616
BbvI GCAGC 4 cut(s) 256, 445, 496, 952
BccI CCATC 2 cut(s) 226, 1071
BcgI CGANNNNNNTGC 2 cut(s) 572, 606
BciT130I CCWGG 3 cut(s) 348, 489, 609
BclI TGATCA 2 cut(s) 478, 838
BcoDI GTCTC 7 cut(s) 151, 248, 345, 421, 578, 787, 1060
BfaI CTAG 1 cut(s) 1159
BfmI CTRYAG 2 cut(s) 303, 903
BisI GCNGC 4 cut(s) 270, 459, 510, 941
BlpI GCTNAGC 1 cut(s) 469
BlsI GCNGC 4 cut(s) 271, 460, 511, 942
Bme1390I CCNGG 3 cut(s) 348, 489, 609
Bme18I GGWCC 3 cut(s) 314, 491, 1107
BmeRI GACNNNNNGTC 1 cut(s) 1017
BmgT120I GGNCC 3 cut(s) 314, 491, 1107
BmiI GGNNCC 2 cut(s) 888, 1108
BmrFI CCNGG 3 cut(s) 348, 489, 609
BmsI GCATC 5 cut(s) 280, 541, 580, 1120, 1146
BpiI GAAGAC 2 cut(s) 56, 1031
BpmI CTGGAG 2 cut(s) 534, 630
Bpu1102I GCTNAGC 1 cut(s) 469
BpuEI CTTGAG 1 cut(s) 258
BsaHI GRCGYC 1 cut(s) 210
BsaI GGTCTC 1 cut(s) 345
BsaJI CCNNGG 1 cut(s) 617
BsaWI WCCGGW 1 cut(s) 1096
BsaXI ACNNNNNCTCC 4 cut(s) 28, 58, 577, 607
Bsc4I CCNNNNNNNGG 4 cut(s) 353, 1096, 1103, 1116
Bse118I RCCGGY 1 cut(s) 889
Bse21I CCTNAGG 1 cut(s) 194
Bse3DI GCAATG 1 cut(s) 629
BseBI CCWGG 3 cut(s) 348, 489, 609
BseDI CCNNGG 1 cut(s) 617
BseGI GGATG 3 cut(s) 532, 607, 1135
BseLI CCNNNNNNNGG 4 cut(s) 353, 1096, 1103, 1116
BseMI GCAATG 1 cut(s) 629
BseMII CTCAG 3 cut(s) 106, 483, 894
BseRI GAGGAG 2 cut(s) 14, 43
BseXI GCAGC 4 cut(s) 256, 445, 496, 952
BshNI GGYRCC 1 cut(s) 886
BsiHKAI GWGCWC 1 cut(s) 616
BsiSI CCGG 2 cut(s) 890, 1097
BslFI GGGAC 1 cut(s) 1120
BslI CCNNNNNNNGG 4 cut(s) 353, 1096, 1103, 1116
BsmAI GTCTC 7 cut(s) 151, 248, 345, 421, 578, 787, 1060
BsmBI CGTCTC 2 cut(s) 787, 1060
BsmFI GGGAC 1 cut(s) 1120
BsmI GAATGC 2 cut(s) 875, 1047
Bso31I GGTCTC 1 cut(s) 345
Bsp1286I GDGCHC 3 cut(s) 247, 470, 616
Bsp143I GATC 5 cut(s) 478, 823, 838, 1000, 1155
Bsp1720I GCTNAGC 1 cut(s) 469
BspACI CCGC 3 cut(s) 312, 357, 1064
BspCNI CTCAG 3 cut(s) 105, 482, 893
BspLI GGNNCC 2 cut(s) 888, 1108
BspT107I GGYRCC 1 cut(s) 886
BspTNI GGTCTC 1 cut(s) 345
BsrDI GCAATG 1 cut(s) 629
BsrFI RCCGGY 1 cut(s) 889
BssAI RCCGGY 1 cut(s) 889
BssECI CCNNGG 1 cut(s) 617
BssMI GATC 5 cut(s) 478, 823, 838, 1000, 1155
BssNI GRCGYC 1 cut(s) 210
BssT1I CCWWGG 1 cut(s) 617
Bst2UI CCWGG 3 cut(s) 348, 489, 609
Bst4CI ACNGT 3 cut(s) 281, 785, 960
Bst6I CTCTTC 4 cut(s) 34, 1065, 1095, 1107
BstACI GRCGYC 1 cut(s) 210
BstAPI GCANNNNNTGC 1 cut(s) 961
BstC8I GCNNGC 3 cut(s) 708, 891, 1135
BstDEI CTNAG 5 cut(s) 92, 194, 318, 469, 880
BstF5I GGATG 3 cut(s) 532, 607, 1135
BstHHI GCGC 1 cut(s) 875
BstKTI GATC 5 cut(s) 481, 826, 841, 1003, 1158
BstMAI GTCTC 7 cut(s) 151, 248, 345, 421, 578, 787, 1060
BstMBI GATC 5 cut(s) 478, 823, 838, 1000, 1155
BstMWI GCNNNNNNNGC 4 cut(s) 299, 455, 961, 970
BstNI CCWGG 3 cut(s) 348, 489, 609
BstSCI CCNGG 3 cut(s) 346, 487, 607
BstSFI CTRYAG 2 cut(s) 303, 903
BstV1I GCAGC 4 cut(s) 256, 445, 496, 952
BstV2I GAAGAC 2 cut(s) 56, 1031
BstX2I RGATCY 1 cut(s) 1155
BstXI CCANNNNNNTGG 1 cut(s) 624
BstYI RGATCY 1 cut(s) 1155
Bsu36I CCTNAGG 1 cut(s) 194
BtgZI GCGATG 1 cut(s) 151
BtsCI GGATG 3 cut(s) 532, 607, 1135
BtsIMutI CAGTG 4 cut(s) 286, 642, 864, 888
Cac8I GCNNGC 3 cut(s) 708, 891, 1135
CaiI CAGNNNCTG 1 cut(s) 476
CfoI GCGC 1 cut(s) 875
Cfr10I RCCGGY 1 cut(s) 889
Cfr13I GGNCC 3 cut(s) 314, 491, 1107
CsiI ACCWGGT 2 cut(s) 346, 487
CspCI CAANNNNNGTGG 2 cut(s) 864, 899
CviAII CATG 2 cut(s) 925, 951
DdeI CTNAG 5 cut(s) 92, 194, 318, 469, 880
DpnI GATC 5 cut(s) 480, 825, 840, 1002, 1157
DpnII GATC 5 cut(s) 478, 823, 838, 1000, 1155
DraI TTTAAA 1 cut(s) 325
DrdI GACNNNNNNGTC 1 cut(s) 389
DriI GACNNNNNGTC 1 cut(s) 1017
DseDI GACNNNNNNGTC 1 cut(s) 389
Eam1104I CTCTTC 4 cut(s) 34, 1065, 1095, 1107
Eam1105I GACNNNNNGTC 1 cut(s) 1017
EarI CTCTTC 4 cut(s) 34, 1065, 1095, 1107
Ecl136II GAGCTC 1 cut(s) 614
Eco130I CCWWGG 1 cut(s) 617
Eco24I GRGCYC 3 cut(s) 247, 470, 616
Eco31I GGTCTC 1 cut(s) 345
Eco47I GGWCC 3 cut(s) 314, 491, 1107
Eco53kI GAGCTC 1 cut(s) 614
Eco57I CTGAAG 2 cut(s) 465, 1043
Eco81I CCTNAGG 1 cut(s) 194
EcoICRI GAGCTC 1 cut(s) 614
EcoRI GAATTC 1 cut(s) 983
EcoRII CCWGG 3 cut(s) 346, 487, 607
EcoT14I CCWWGG 1 cut(s) 617
EcoT38I GRGCYC 3 cut(s) 247, 470, 616
ErhI CCWWGG 1 cut(s) 617
Esp3I CGTCTC 2 cut(s) 787, 1060
FaeI CATG 2 cut(s) 928, 954
FaqI GGGAC 1 cut(s) 1120
FatI CATG 2 cut(s) 924, 950
FbaI TGATCA 2 cut(s) 478, 838
FblI GTMKAC 1 cut(s) 17
Fnu4HI GCNGC 4 cut(s) 270, 459, 510, 941
FokI GGATG 3 cut(s) 519, 614, 1142
FriOI GRGCYC 3 cut(s) 247, 470, 616
Fsp4HI GCNGC 4 cut(s) 270, 459, 510, 941
FspBI CTAG 1 cut(s) 1159
FspI TGCGCA 1 cut(s) 874
GlaI GCGC 1 cut(s) 874
GluI GCNGC 4 cut(s) 270, 459, 510, 941
GsuI CTGGAG 2 cut(s) 534, 630
HapII CCGG 2 cut(s) 890, 1097
HhaI GCGC 1 cut(s) 875
Hin1I GRCGYC 1 cut(s) 210
Hin1II CATG 2 cut(s) 928, 954
Hin6I GCGC 1 cut(s) 873
HinP1I GCGC 1 cut(s) 873
HincII GTYRAC 1 cut(s) 18
HindII GTYRAC 1 cut(s) 18
HindIII AAGCTT 1 cut(s) 447
HinfI GANTC 7 cut(s) 19, 96, 200, 363, 516, 697, 744
HpaII CCGG 2 cut(s) 890, 1097
Hpy166II GTNNAC 1 cut(s) 18
Hpy188I TCNGA 6 cut(s) 95, 113, 205, 478, 483, 1023
Hpy188III TCNNGA 8 cut(s) 167, 253, 275, 342, 443, 583, 748, 842
Hpy8I GTNNAC 1 cut(s) 18
Hpy99I CGWCG 2 cut(s) 19, 969
HpyAV CCTTC 4 cut(s) 302, 738, 742, 1141
HpyCH4III ACNGT 3 cut(s) 281, 785, 960
HpyCH4IV ACGT 1 cut(s) 210
HpyCH4V TGCA 6 cut(s) 293, 379, 461, 532, 562, 1137
HpyF10VI GCNNNNNNNGC 4 cut(s) 299, 455, 961, 970
HpyF3I CTNAG 5 cut(s) 92, 194, 318, 469, 880
HpySE526I ACGT 1 cut(s) 210
Hsp92I GRCGYC 1 cut(s) 210
Hsp92II CATG 2 cut(s) 928, 954
HspAI GCGC 1 cut(s) 873
KroI GCCGGC 1 cut(s) 889
KroNI GCCGGC 1 cut(s) 891
Ksp22I TGATCA 2 cut(s) 478, 838
Kzo9I GATC 5 cut(s) 478, 823, 838, 1000, 1155
LmnI GCTCC 4 cut(s) 49, 598, 611, 619
Lsp1109I GCAGC 4 cut(s) 256, 445, 496, 952
LweI GCATC 5 cut(s) 280, 541, 580, 1120, 1146
MabI ACCWGGT 2 cut(s) 346, 487
MaeI CTAG 1 cut(s) 1159
MaeII ACGT 1 cut(s) 210
MaeIII GTNAC 2 cut(s) 211, 713
MalI GATC 5 cut(s) 480, 825, 840, 1002, 1157
MboI GATC 5 cut(s) 478, 823, 838, 1000, 1155
MfeI CAATTG 2 cut(s) 150, 294
MflI RGATCY 1 cut(s) 1155
MhlI GDGCHC 3 cut(s) 247, 470, 616
MluCI AATT 6 cut(s) 150, 294, 568, 719, 929, 983
MlyI GAGTC 2 cut(s) 13, 525
MnlI CCTC 9 cut(s) 35, 64, 94, 450, 791, 800, 962, 1025, 1096
MroNI GCCGGC 1 cut(s) 889
MroXI GAANNNNTTC 1 cut(s) 849
MseI TTAA 3 cut(s) 324, 411, 828
MslI CAYNNNNRTG 1 cut(s) 786
MspI CCGG 2 cut(s) 890, 1097
MspR9I CCNGG 3 cut(s) 348, 489, 609
MunI CAATTG 2 cut(s) 150, 294
Mva1269I GAATGC 2 cut(s) 875, 1047
MvaI CCWGG 3 cut(s) 348, 489, 609
MwoI GCNNNNNNNGC 4 cut(s) 299, 455, 961, 970
NaeI GCCGGC 1 cut(s) 891
NdeII GATC 5 cut(s) 478, 823, 838, 1000, 1155
NgoMIV GCCGGC 1 cut(s) 889
NlaIII CATG 2 cut(s) 928, 954
NlaIV GGNNCC 2 cut(s) 888, 1108
NmuCI GTSAC 2 cut(s) 211, 713
NsbI TGCGCA 1 cut(s) 874
PctI GAATGC 2 cut(s) 875, 1047
PdiI GCCGGC 1 cut(s) 891
PdmI GAANNNNTTC 1 cut(s) 849
PfeI GAWTC 5 cut(s) 96, 200, 363, 697, 744
PfoI TCCNGGA 1 cut(s) 607
PkrI GCNGC 4 cut(s) 271, 460, 511, 942
PleI GAGTC 2 cut(s) 13, 524
PpsI GAGTC 2 cut(s) 13, 524
Psp124BI GAGCTC 1 cut(s) 616
Psp6I CCWGG 3 cut(s) 346, 487, 607
PspGI CCWGG 3 cut(s) 346, 487, 607
PspN4I GGNNCC 2 cut(s) 888, 1108
PspPI GGNCC 3 cut(s) 314, 491, 1107
PstNI CAGNNNCTG 1 cut(s) 476
PsuI RGATCY 1 cut(s) 1155
RseI CAYNNNNRTG 1 cut(s) 786
SacI GAGCTC 1 cut(s) 616
SalI GTCGAC 1 cut(s) 16
SaqAI TTAA 3 cut(s) 324, 411, 828
SatI GCNGC 4 cut(s) 270, 459, 510, 941
Sau3AI GATC 5 cut(s) 478, 823, 838, 1000, 1155
Sau96I GGNCC 3 cut(s) 314, 491, 1107
SchI GAGTC 2 cut(s) 13, 525
ScrFI CCNGG 3 cut(s) 348, 489, 609
SduI GDGCHC 3 cut(s) 247, 470, 616
SexAI ACCWGGT 2 cut(s) 346, 487
SfaNI GCATC 5 cut(s) 280, 541, 580, 1120, 1146
SfcI CTRYAG 2 cut(s) 303, 903
SinI GGWCC 3 cut(s) 314, 491, 1107
SmiMI CAYNNNNRTG 1 cut(s) 786
SmlI CTYRAG 1 cut(s) 273
SmoI CTYRAG 1 cut(s) 273
Sse9I AATT 6 cut(s) 150, 294, 568, 719, 929, 983
SsiI CCGC 3 cut(s) 312, 357, 1064
SspMI CTAG 1 cut(s) 1159
SstI GAGCTC 1 cut(s) 616
StyD4I CCNGG 3 cut(s) 346, 487, 607
StyI CCWWGG 1 cut(s) 617
TaaI ACNGT 3 cut(s) 281, 785, 960
TaiI ACGT 1 cut(s) 213
TaqI TCGA 6 cut(s) 17, 252, 343, 582, 695, 981
TaqII GACCGA 1 cut(s) 1124
TasI AATT 6 cut(s) 150, 294, 568, 719, 929, 983
TfiI GAWTC 5 cut(s) 96, 200, 363, 697, 744
Tru1I TTAA 3 cut(s) 324, 411, 828
Tru9I TTAA 3 cut(s) 324, 411, 828
TscAI CASTG 4 cut(s) 286, 649, 871, 888
TseFI GTSAC 2 cut(s) 211, 713
TseI GCWGC 4 cut(s) 269, 458, 509, 940
Tsp45I GTSAC 2 cut(s) 211, 713
TspDTI ATGAA 1 cut(s) 56
TspGWI ACGGA 2 cut(s) 226, 489
TspRI CASTG 4 cut(s) 286, 649, 871, 888
VpaK11BI GGWCC 3 cut(s) 314, 491, 1107
XapI RAATTY 3 cut(s) 719, 929, 983
XcmI CCANNNNNNNNNTGG 1 cut(s) 624
XmiI GTMKAC 1 cut(s) 17
XmnI GAANNNNTTC 1 cut(s) 849
XspI CTAG 1 cut(s) 1159
ZraI GACGTC 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.