RLG00000020666

Coproporphyrinogen-III oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0011336
Physical Location & Seq
Forward (+)
67305180 .. 67309017
3838 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020666

Sequence Viewer

Length: 1188 bp
ATGCCGCCGCCAGCCACACTCTCATCTTCTTCCTCCTTCACACTCCTCTCCTCTTCTCCCTCCCCATCTCCAAAGCTCGCATCTTTCCACTCCAAACACCACTCCTTCCACTCCGCCACGCGCTTCACGCGCCTCCGCCTCCGCCTCCGCCGCCGCCTCGCTCGCTCACTCACTCCGCGATGCGCCGTCATCGAGAAAGAGACGCCGGAGACGGAAAAGCCTGATACTTTCCTACGAGAGTCGTCGTCGCCGACCTCAGTCCGGGCGCGGTTCGAGAAGATGATAAGGGAGGCGCAGGACACAGTGTGTCAGGCCATCGCCGCCGCGGATGGCGGAGGCCAGTTTAAGGAGGACGTGTGGTCGAGGCCGGGCGGCGGTGGAGGTATCAGTAGAGTCCTCCAAAACGGCGCCGTTTGGGAGAAGGCTGGGGTTAATGTGTCTGTTGTGTATGGAGTTATGCCTCCTGAGGCTTATAGAGCTGCCAAGGGCGCCGCCACTGATCACAAGCCCGGCCCGGTTCCGTTCTTCGCCGCCGGAATCAGCTCGGTTTTGCATCCAAAGAACCCTTTTGCTCCTACTTTGCATTTCAATTATCGTTATTTCGAAACAGATGCTCCCCAAGATGCTCTTGGAGCACCTAGGCAATGGTGGTTTGGAGGTGGAACTGACTTGACGCCTGCTTATATTTTTGAGGAGGATGTTAAACATTTTCATTCAGTTCAGAAAAGTGCTTGCGACAAATTTGATCCTACCTTCTATCCACGGTTCAAGAAATGGTGTGATGATTATTTCTATATCAAGCATCGTGATGAGAGACGAGGGCTTGGGGGAATATTTTTTGATGATCTAAATGACTATGATCAGGAGATGCTTCTTTCTTTTGCCACTGAATGTGCAAACTCTGTGGTCCCTGCTTATGTACCAATTATAGAGAAAAGGAAGGATACACCATTTACAGAGGACCATAAGGCATGGCAGCAATTGCGAAGAGGGAGATATGTCGAATTCAATTTGGTTTATGATCGTGGAACAACTTTTGGACTAAAGACAGGAGGTCGAATTGAGAGTATTCTTGTCTCTCTTCCACTGACTGCTCGGTGGGAATACGACCATCAACCGAATTCTGCTGAGACTAAAGTGTCTAAGTACACAACTACTGCTGCACTCTGCAAAATAGTCGGAGACTGA

Protein Analysis

396

Amino Acids

44.34

Weight (kDa)

8.96

Isoelectric Point (pI)

59.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Coprogen_oxidas PF01218 91 - 382 1.5e-131 Coproporphyrinogen III oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011336)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03475 AT4G03205 AT4G03205
fragaria_vesca FvH4_6g39170
malus_domestica MD09G1144600.v1.1 MD17G1131200.v1.1
prunus_persica Prupe.3G183400_v2.0.a1
pyrus_communis pycom09g06400 pycom17g12400
rosa_chinensis RchiOBHm_Chr2g0153001
rosa_laevigata RLG00000020666
rosa_multiflora Rmu_sc0000218.1_g000004 Rmu_sc0000218.1_g000005
rosa_roxburghii Rroxscaffold_2G00095310
rosa_rugosa Rorug02G0436700
rosa_samantha Rh2AG498700 Rh2BG510000 Rh2CG485000 Rh2DG521600
rosa_wichuraiana Rw2G040930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1138
AccB1I GGYRCC 2 cut(s) 407, 488
AccII CGCG 5 cut(s) 121, 130, 178, 268, 326
AclWI GGATC 1 cut(s) 740
AcsI RAATTY 3 cut(s) 740, 1004, 1120
AcyI GRCGYC 4 cut(s) 203, 408, 489, 674
AdeI CACNNNGTG 1 cut(s) 306
AfaI GTAC 2 cut(s) 921, 1148
AfiI CCNNNNNNNGG 3 cut(s) 261, 346, 374
AflIII ACRYGT 1 cut(s) 354
AgsI TTSAA 3 cut(s) 589, 769, 1009
AhdI GACNNNNNGTC 2 cut(s) 358, 1053
AjiI CACGTC 1 cut(s) 355
AluBI AGCT 3 cut(s) 76, 479, 543
AluI AGCT 3 cut(s) 76, 479, 543
Alw21I GWGCWC 1 cut(s) 637
Alw26I GTCTC 6 cut(s) 194, 203, 808, 1081, 1124, 1176
AlwI GGATC 1 cut(s) 740
AoxI GGCC 4 cut(s) 312, 337, 365, 511
ApeKI GCWGC 3 cut(s) 479, 976, 1160
ApoI RAATTY 3 cut(s) 740, 1004, 1120
AspA2I CCTAGG 1 cut(s) 638
AspLEI GCGC 7 cut(s) 123, 132, 185, 268, 295, 410, 491
AspS9I GGNCC 3 cut(s) 512, 907, 961
AsuC2I CCSGG 4 cut(s) 263, 369, 510, 515
AsuII TTCGAA 1 cut(s) 603
AvaII GGWCC 2 cut(s) 907, 961
AvrII CCTAGG 1 cut(s) 638
AxyI CCTNAGG 1 cut(s) 465
BanI GGYRCC 2 cut(s) 407, 488
Bbv12I GWGCWC 1 cut(s) 637
BbvI GCAGC 3 cut(s) 466, 988, 1147
BccI CCATC 4 cut(s) 73, 323, 323, 1119
BceAI ACGGC 3 cut(s) 170, 395, 421
BcgI CGANNNNNNTGC 3 cut(s) 593, 627, 1159
BciVI GTATCC 1 cut(s) 937
BclI TGATCA 2 cut(s) 499, 859
BcnI CCSGG 4 cut(s) 263, 369, 510, 515
BcoDI GTCTC 6 cut(s) 194, 203, 808, 1081, 1124, 1176
BfaI CTAG 1 cut(s) 639
BfoI RGCGCY 2 cut(s) 411, 492
BfuI GTATCC 1 cut(s) 937
BlnI CCTAGG 1 cut(s) 638
Bme1390I CCNGG 4 cut(s) 263, 369, 510, 515
Bme18I GGWCC 2 cut(s) 907, 961
BmeRI GACNNNNNGTC 2 cut(s) 358, 1053
BmgBI CACGTC 1 cut(s) 355
BmgT120I GGNCC 3 cut(s) 512, 907, 961
BmiI GGNNCC 4 cut(s) 409, 490, 519, 909
BmrFI CCNGG 4 cut(s) 263, 369, 510, 515
BmsI GCATC 7 cut(s) 89, 170, 562, 601, 613, 811, 858
BoxI GACNNNNGTC 1 cut(s) 257
Bpu14I TTCGAA 1 cut(s) 603
BpuMI CCSGG 4 cut(s) 263, 369, 510, 515
BsaHI GRCGYC 4 cut(s) 203, 408, 489, 674
BsaJI CCNNGG 4 cut(s) 324, 483, 638, 761
BsaXI ACNNNNNCTCC 4 cut(s) 341, 371, 598, 628
Bsc4I CCNNNNNNNGG 3 cut(s) 261, 346, 374
Bse1I ACTGG 1 cut(s) 340
Bse21I CCTNAGG 1 cut(s) 465
Bse3DI GCAATG 1 cut(s) 650
BseDI CCNNGG 4 cut(s) 324, 483, 638, 761
BseGI GGATG 3 cut(s) 334, 553, 703
BseLI CCNNNNNNNGG 3 cut(s) 261, 346, 374
BseMI GCAATG 1 cut(s) 650
BseMII CTCAG 3 cut(s) 270, 456, 1119
BseNI ACTGG 1 cut(s) 340
BseRI GAGGAG 3 cut(s) 35, 40, 707
BseXI GCAGC 3 cut(s) 466, 988, 1147
BseYI CCCAGC 1 cut(s) 425
BsgI GTGCAG 1 cut(s) 1146
Bsh1236I CGCG 5 cut(s) 121, 130, 178, 268, 326
BshFI GGCC 4 cut(s) 314, 339, 367, 513
BshNI GGYRCC 2 cut(s) 407, 488
BsiHKAI GWGCWC 1 cut(s) 637
BsiSI CCGG 6 cut(s) 206, 262, 368, 510, 515, 534
BslFI GGGAC 1 cut(s) 893
BslI CCNNNNNNNGG 3 cut(s) 261, 346, 374
BsmAI GTCTC 6 cut(s) 194, 203, 808, 1081, 1124, 1176
BsmBI CGTCTC 3 cut(s) 194, 203, 808
BsmFI GGGAC 1 cut(s) 893
BsnI GGCC 4 cut(s) 314, 339, 367, 513
Bsp119I TTCGAA 1 cut(s) 603
Bsp1286I GDGCHC 1 cut(s) 637
Bsp143I GATC 5 cut(s) 499, 745, 844, 859, 1021
BspANI GGCC 4 cut(s) 314, 339, 367, 513
BspCNI CTCAG 3 cut(s) 269, 457, 1120
BspFNI CGCG 5 cut(s) 121, 130, 178, 268, 326
BspLI GGNNCC 4 cut(s) 409, 490, 519, 909
BspPI GGATC 1 cut(s) 740
BspT104I TTCGAA 1 cut(s) 603
BspT107I GGYRCC 2 cut(s) 407, 488
BsrDI GCAATG 1 cut(s) 650
BsrI ACTGG 1 cut(s) 340
BssECI CCNNGG 4 cut(s) 324, 483, 638, 761
BssMI GATC 5 cut(s) 499, 745, 844, 859, 1021
BssNI GRCGYC 4 cut(s) 203, 408, 489, 674
BssT1I CCWWGG 2 cut(s) 483, 638
Bst4CI ACNGT 2 cut(s) 304, 765
Bst6I CTCTTC 3 cut(s) 58, 982, 1086
BstACI GRCGYC 4 cut(s) 203, 408, 489, 674
BstAPI GCANNNNNTGC 1 cut(s) 982
BstBI TTCGAA 1 cut(s) 603
BstC8I GCNNGC 5 cut(s) 12, 78, 163, 678, 733
BstDEI CTNAG 4 cut(s) 256, 465, 1128, 1143
BstDSI CCRYGG 2 cut(s) 324, 761
BstF5I GGATG 3 cut(s) 334, 553, 703
BstFNI CGCG 5 cut(s) 121, 130, 178, 268, 326
BstH2I RGCGCY 2 cut(s) 411, 492
BstHHI GCGC 7 cut(s) 123, 132, 185, 268, 295, 410, 491
BstKTI GATC 5 cut(s) 502, 748, 847, 862, 1024
BstMAI GTCTC 6 cut(s) 194, 203, 808, 1081, 1124, 1176
BstMBI GATC 5 cut(s) 499, 745, 844, 859, 1021
BstMWI GCNNNNNNNGC 9 cut(s) 127, 129, 150, 162, 320, 476, 488, 632, 982
BstPAI GACNNNNGTC 1 cut(s) 257
BstSCI CCNGG 4 cut(s) 261, 367, 508, 513
BstUI CGCG 5 cut(s) 121, 130, 178, 268, 326
BstV1I GCAGC 3 cut(s) 466, 988, 1147
Bsu36I CCTNAGG 1 cut(s) 465
BsuI GTATCC 1 cut(s) 937
BsuRI GGCC 4 cut(s) 314, 339, 367, 513
BtgI CCRYGG 2 cut(s) 324, 761
BtgZI GCGATG 2 cut(s) 193, 301
BtrI CACGTC 1 cut(s) 355
BtsCI GGATG 3 cut(s) 334, 553, 703
BtsIMutI CAGTG 4 cut(s) 309, 495, 885, 1085
Cac8I GCNNGC 5 cut(s) 12, 78, 163, 678, 733
CfoI GCGC 7 cut(s) 123, 132, 185, 268, 295, 410, 491
Cfr13I GGNCC 3 cut(s) 512, 907, 961
Cfr42I CCGCGG 1 cut(s) 327
CseI GACGC 2 cut(s) 211, 682
Csp6I GTAC 2 cut(s) 920, 1147
CspCI CAANNNNNGTGG 2 cut(s) 885, 920
CviAII CATG 1 cut(s) 972
CviQI GTAC 2 cut(s) 920, 1147
DdeI CTNAG 4 cut(s) 256, 465, 1128, 1143
DinI GGCGCC 2 cut(s) 409, 490
DpnI GATC 5 cut(s) 501, 747, 846, 861, 1023
DpnII GATC 5 cut(s) 499, 745, 844, 859, 1021
DraIII CACNNNGTG 1 cut(s) 306
DrdI GACNNNNNNGTC 1 cut(s) 1138
DriI GACNNNNNGTC 2 cut(s) 358, 1053
DseDI GACNNNNNNGTC 1 cut(s) 1138
Eam1104I CTCTTC 3 cut(s) 58, 982, 1086
Eam1105I GACNNNNNGTC 2 cut(s) 358, 1053
EarI CTCTTC 3 cut(s) 58, 982, 1086
EciI GGCGGA 5 cut(s) 103, 125, 131, 137, 348
Eco130I CCWWGG 2 cut(s) 483, 638
Eco47I GGWCC 2 cut(s) 907, 961
Eco81I CCTNAGG 1 cut(s) 465
EcoRI GAATTC 2 cut(s) 1004, 1120
EcoT14I CCWWGG 2 cut(s) 483, 638
EgeI GGCGCC 2 cut(s) 409, 490
EheI GGCGCC 2 cut(s) 409, 490
ErhI CCWWGG 2 cut(s) 483, 638
Esp3I CGTCTC 3 cut(s) 194, 203, 808
FaeI CATG 1 cut(s) 975
FalI AAGNNNNNCTT 2 cut(s) 612, 644
FaqI GGGAC 1 cut(s) 893
FatI CATG 1 cut(s) 971
FbaI TGATCA 2 cut(s) 499, 859
FokI GGATG 3 cut(s) 341, 540, 710
FspBI CTAG 1 cut(s) 639
GlaI GCGC 7 cut(s) 122, 131, 184, 267, 294, 409, 490
GsaI CCCAGC 1 cut(s) 429
HaeII RGCGCY 2 cut(s) 411, 492
HaeIII GGCC 4 cut(s) 314, 339, 367, 513
HapII CCGG 6 cut(s) 206, 262, 368, 510, 515, 534
HgaI GACGC 2 cut(s) 211, 682
HhaI GCGC 7 cut(s) 123, 132, 185, 268, 295, 410, 491
Hin1I GRCGYC 4 cut(s) 203, 408, 489, 674
Hin1II CATG 1 cut(s) 975
Hin6I GCGC 7 cut(s) 121, 130, 183, 266, 293, 408, 489
HinP1I GCGC 7 cut(s) 121, 130, 183, 266, 293, 408, 489
HinfI GANTC 3 cut(s) 239, 393, 537
HpaII CCGG 6 cut(s) 206, 262, 368, 510, 515, 534
Hpy166II GTNNAC 1 cut(s) 1149
Hpy188I TCNGA 2 cut(s) 723, 1181
Hpy188III TCNNGA 6 cut(s) 193, 274, 464, 769, 806, 863
Hpy8I GTNNAC 1 cut(s) 1149
Hpy99I CGWCG 2 cut(s) 247, 250
HpyAV CCTTC 5 cut(s) 46, 115, 415, 763, 934
HpyCH4III ACNGT 2 cut(s) 304, 765
HpyCH4IV ACGT 1 cut(s) 354
HpyCH4V TGCA 5 cut(s) 553, 583, 896, 1163, 1170
HpyF10VI GCNNNNNNNGC 9 cut(s) 127, 129, 150, 162, 320, 476, 488, 632, 982
HpyF3I CTNAG 4 cut(s) 256, 465, 1128, 1143
HpySE526I ACGT 1 cut(s) 354
Hsp92I GRCGYC 4 cut(s) 203, 408, 489, 674
Hsp92II CATG 1 cut(s) 975
HspAI GCGC 7 cut(s) 121, 130, 183, 266, 293, 408, 489
KasI GGCGCC 2 cut(s) 407, 488
Ksp22I TGATCA 2 cut(s) 499, 859
KspI CCGCGG 1 cut(s) 327
Kzo9I GATC 5 cut(s) 499, 745, 844, 859, 1021
LmnI GCTCC 3 cut(s) 577, 619, 632
Lsp1109I GCAGC 3 cut(s) 466, 988, 1147
LweI GCATC 7 cut(s) 89, 170, 562, 601, 613, 811, 858
MaeI CTAG 1 cut(s) 639
MaeII ACGT 1 cut(s) 354
MalI GATC 5 cut(s) 501, 747, 846, 861, 1023
MboI GATC 5 cut(s) 499, 745, 844, 859, 1021
MboII GAAGA 7 cut(s) 18, 21, 45, 289, 517, 999, 1073
MfeI CAATTG 1 cut(s) 980
MhlI GDGCHC 1 cut(s) 637
MluCI AATT 8 cut(s) 589, 740, 924, 980, 1004, 1009, 1059, 1120
Mly113I GGCGCC 2 cut(s) 408, 489
MlyI GAGTC 2 cut(s) 248, 402
MmeI TCCRAC 1 cut(s) 1159
MseI TTAA 3 cut(s) 345, 432, 702
MslI CAYNNNNRTG 1 cut(s) 807
MspA1I CMGCKG 1 cut(s) 326
MspI CCGG 6 cut(s) 206, 262, 368, 510, 515, 534
MspR9I CCNGG 4 cut(s) 263, 369, 510, 515
MunI CAATTG 1 cut(s) 980
MvnI CGCG 5 cut(s) 121, 130, 178, 268, 326
MwoI GCNNNNNNNGC 9 cut(s) 127, 129, 150, 162, 320, 476, 488, 632, 982
NarI GGCGCC 2 cut(s) 408, 489
NciI CCSGG 4 cut(s) 263, 369, 510, 515
NdeII GATC 5 cut(s) 499, 745, 844, 859, 1021
NlaIII CATG 1 cut(s) 975
NlaIV GGNNCC 4 cut(s) 409, 490, 519, 909
NspV TTCGAA 1 cut(s) 603
PcsI WCGNNNNNNNCGW 1 cut(s) 248
PfeI GAWTC 1 cut(s) 537
PleI GAGTC 2 cut(s) 247, 401
PluTI GGCGCC 2 cut(s) 411, 492
PpsI GAGTC 2 cut(s) 247, 401
PshAI GACNNNNGTC 1 cut(s) 257
PspFI CCCAGC 1 cut(s) 425
PspN4I GGNNCC 4 cut(s) 409, 490, 519, 909
PspPI GGNCC 3 cut(s) 512, 907, 961
RsaI GTAC 2 cut(s) 921, 1148
RsaNI GTAC 2 cut(s) 920, 1147
RseI CAYNNNNRTG 1 cut(s) 807
SacII CCGCGG 1 cut(s) 327
SaqAI TTAA 3 cut(s) 345, 432, 702
Sau3AI GATC 5 cut(s) 499, 745, 844, 859, 1021
Sau96I GGNCC 3 cut(s) 512, 907, 961
SchI GAGTC 2 cut(s) 248, 402
ScrFI CCNGG 4 cut(s) 263, 369, 510, 515
SduI GDGCHC 1 cut(s) 637
SfaNI GCATC 7 cut(s) 89, 170, 562, 601, 613, 811, 858
SfoI GGCGCC 2 cut(s) 409, 490
Sfr303I CCGCGG 1 cut(s) 327
SfuI TTCGAA 1 cut(s) 603
SgrBI CCGCGG 1 cut(s) 327
SinI GGWCC 2 cut(s) 907, 961
SmiMI CAYNNNNRTG 1 cut(s) 807
Sse9I AATT 8 cut(s) 589, 740, 924, 980, 1004, 1009, 1059, 1120
SspDI GGCGCC 2 cut(s) 407, 488
SspI AATATT 1 cut(s) 834
SspMI CTAG 1 cut(s) 639
StyD4I CCNGG 4 cut(s) 261, 367, 508, 513
StyI CCWWGG 2 cut(s) 483, 638
TaaI ACNGT 2 cut(s) 304, 765
TaiI ACGT 1 cut(s) 357
TaqI TCGA 6 cut(s) 192, 273, 362, 603, 1002, 1057
TasI AATT 8 cut(s) 589, 740, 924, 980, 1004, 1009, 1059, 1120
TatI WGTACW 1 cut(s) 1146
TauI GCSGC 9 cut(s) 7, 10, 153, 156, 323, 326, 375, 494, 533
TfiI GAWTC 1 cut(s) 537
Tru1I TTAA 3 cut(s) 345, 432, 702
Tru9I TTAA 3 cut(s) 345, 432, 702
TscAI CASTG 4 cut(s) 309, 502, 892, 1092
TseI GCWGC 3 cut(s) 479, 976, 1160
TspDTI ATGAA 1 cut(s) 701
TspGWI ACGGA 2 cut(s) 227, 510
TspRI CASTG 4 cut(s) 309, 502, 892, 1092
VpaK11BI GGWCC 2 cut(s) 907, 961
XapI RAATTY 3 cut(s) 740, 1004, 1120
XcmI CCANNNNNNNNNTGG 1 cut(s) 626
XmaJI CCTAGG 1 cut(s) 638
XspI CTAG 1 cut(s) 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.