FvH4_6g39170

Coproporphyrinogen-III oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011336
Physical Location & Seq
Forward (+)
30986850 .. 30990298
3449 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g39170.t1

Sequence Viewer

Length: 1161 bp
ATGCCGCCACCAGCCACACTCTCAGCTTCCTCCTCCTTCACCCTCCTCTCCCCACCTCCAAAGCTCTCTTCCTTCCTCCCCAATCCCCACTCCTTCCACTCCTCCACGCGCTACACGCCCTTCACGCGCCTCCGCCACTCCCCCTCTTTCACTCCCCGATGCGCCGTCATCGAGAAGGAGACGCCGGAGACGGAAAAGCCCGAGACTTTCCTCCGCGAGTCGTCGTCGCCGACCTCCGTCCGCGCGCGGTTCGAGAAGATGATACGGGAGGCGCAGGACACTGTGTGTGAGGCCATCGCCGCCGCGGACGGCGTCGGCACGTTTAAGGAGGACGTGTGGTCGAGGCCCGGCGGCGGCGGCGGGATTAGTAGAGTCTTGCAAGACGGCGCCGTTTGGGAGAAGGCTGGGGTTAATGTGTCTGTTGTGTATGGGGTTATGCCTCCTGAGGCTTATAGAGCTGCCAAGGGCGCCGCCGCTGATCAAAAACCCGGGCCGGTTCCGTTCTTCGCCGCCGGAATTAGCTCGGTTATGCATCCAAAGAACCCATTTGCGCCTACATTGCACTTCAATTATCGGTATTTCGAGACCGATGCTCCCCAAGATGCTCCTGGAGCACCTAGGCAATGGTGGTTTGGAGGTGGAACTGACTTGACGCCTGCTTATATTTTTGATGAGGATGTTAAACATTTTCATTCAGTTCAGAAAAGTGCGTGTGACAAATTTGATCCGTCCTTCTATCCTCGGTTCAAGAAATGGTGTGATGATTATTTCTATATCAAGCATCGTGATGAGAGACGAGGGCTTGGGGGAATATTTTTTGATGATCTAAATGACTATGATCAGGAGATGCTTCTTTCTTTTGCCACTGAATGTGCCAACTCTGTGGTCCCTGCCTACGTACCAATTATAGAGAAAAGGAAGGATACACCATTTACGGACGACCATAAGGCATGGCAGCAATTGCGGAGAGGACGTTATGTTGAGTTCAATTTGGTTTACGATCGTGGAACAACTTTTGGCCTAAAGACAGGAGGTCGAATCGAGAGTATTCTTGTCTCTCTTCCACTGACTGCTCGGTGGGAATATGACCATAAACCAGAAGAGGGAAGCGAAGAATGGAAACTACTAGATGCTTGCATCCACCCAAAGGAATGGGTTTAA

Protein Analysis

387

Amino Acids

43.52

Weight (kDa)

6.15

Isoelectric Point (pI)

59.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Coprogen_oxidas PF01218 84 - 385 4.1e-136 Coproporphyrinogen III oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011336)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03475 AT4G03205 AT4G03205
fragaria_vesca FvH4_6g39170
malus_domestica MD09G1144600.v1.1 MD17G1131200.v1.1
prunus_persica Prupe.3G183400_v2.0.a1
pyrus_communis pycom09g06400 pycom17g12400
rosa_chinensis RchiOBHm_Chr2g0153001
rosa_laevigata RLG00000020666
rosa_multiflora Rmu_sc0000218.1_g000004 Rmu_sc0000218.1_g000005
rosa_roxburghii Rroxscaffold_2G00095310
rosa_rugosa Rorug02G0436700
rosa_samantha Rh2AG498700 Rh2BG510000 Rh2CG485000 Rh2DG521600
rosa_wichuraiana Rw2G040930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 386, 467
AccII CGCG 7 cut(s) 109, 127, 216, 243, 245, 247, 305
AclWI GGATC 1 cut(s) 719
AcsI RAATTY 1 cut(s) 719
AcyI GRCGYC 5 cut(s) 182, 312, 387, 468, 653
AdeI CACNNNGTG 1 cut(s) 285
AfaI GTAC 1 cut(s) 900
AfiI CCNNNNNNNGG 3 cut(s) 353, 1103, 1147
AflIII ACRYGT 1 cut(s) 333
AgsI TTSAA 3 cut(s) 568, 748, 988
AhdI GACNNNNNGTC 2 cut(s) 337, 1032
AjiI CACGTC 1 cut(s) 334
AjnI CCWGG 1 cut(s) 607
AjuI GAANNNNNNNTTGG 2 cut(s) 52, 84
AluBI AGCT 4 cut(s) 26, 64, 458, 522
AluI AGCT 4 cut(s) 26, 64, 458, 522
Alw21I GWGCWC 1 cut(s) 616
Alw26I GTCTC 6 cut(s) 173, 182, 197, 578, 787, 1060
AlwI GGATC 1 cut(s) 719
Ama87I CYCGRG 2 cut(s) 200, 488
AoxI GGCC 4 cut(s) 291, 344, 491, 1018
ApeKI GCWGC 2 cut(s) 458, 955
ApoI RAATTY 1 cut(s) 719
AspA2I CCTAGG 1 cut(s) 617
AspLEI GCGC 9 cut(s) 111, 129, 164, 245, 247, 274, 389, 470, 553
AspS9I GGNCC 3 cut(s) 345, 491, 886
AsuC2I CCSGG 3 cut(s) 348, 489, 490
AsuHPI GGTGA 1 cut(s) 31
AvaI CYCGRG 2 cut(s) 200, 488
AvaII GGWCC 1 cut(s) 886
AvrII CCTAGG 1 cut(s) 617
AxyI CCTNAGG 1 cut(s) 444
BanI GGYRCC 2 cut(s) 386, 467
Bbv12I GWGCWC 1 cut(s) 616
BbvI GCAGC 2 cut(s) 445, 967
BccI CCATC 1 cut(s) 302
BceAI ACGGC 4 cut(s) 149, 325, 374, 400
BcgI CGANNNNNNTGC 2 cut(s) 572, 606
BciT130I CCWGG 1 cut(s) 609
BciVI GTATCC 1 cut(s) 916
BclI TGATCA 2 cut(s) 478, 838
BcnI CCSGG 3 cut(s) 348, 489, 490
BcoDI GTCTC 6 cut(s) 173, 182, 197, 578, 787, 1060
BfaI CTAG 2 cut(s) 618, 1127
BfoI RGCGCY 2 cut(s) 390, 471
BfuI GTATCC 1 cut(s) 916
BlnI CCTAGG 1 cut(s) 617
Bme1390I CCNGG 4 cut(s) 348, 489, 490, 609
Bme18I GGWCC 1 cut(s) 886
BmeRI GACNNNNNGTC 2 cut(s) 337, 1032
BmeT110I CYCGRG 2 cut(s) 200, 488
BmgBI CACGTC 1 cut(s) 334
BmgT120I GGNCC 3 cut(s) 345, 491, 886
BmiI GGNNCC 4 cut(s) 388, 469, 498, 888
BmrFI CCNGG 4 cut(s) 348, 489, 490, 609
BmsI GCATC 8 cut(s) 149, 541, 580, 592, 790, 837, 1120, 1146
BoxI GACNNNNGTC 1 cut(s) 236
BpmI CTGGAG 1 cut(s) 630
BpuMI CCSGG 3 cut(s) 348, 489, 490
BsaAI YACGTR 1 cut(s) 898
BsaHI GRCGYC 5 cut(s) 182, 312, 387, 468, 653
BsaI GGTCTC 1 cut(s) 578
BsaJI CCNNGG 5 cut(s) 303, 462, 488, 617, 740
BsaXI ACNNNNNCTCC 6 cut(s) 320, 350, 577, 607, 958, 988
Bsc4I CCNNNNNNNGG 3 cut(s) 353, 1103, 1147
Bse118I RCCGGY 1 cut(s) 493
Bse21I CCTNAGG 1 cut(s) 444
Bse3DI GCAATG 2 cut(s) 557, 629
BseBI CCWGG 1 cut(s) 609
BseDI CCNNGG 5 cut(s) 303, 462, 488, 617, 740
BseGI GGATG 3 cut(s) 532, 682, 1137
BseLI CCNNNNNNNGG 3 cut(s) 353, 1103, 1147
BseMI GCAATG 2 cut(s) 557, 629
BseMII CTCAG 2 cut(s) 36, 435
BsePI GCGCGC 1 cut(s) 243
BseRI GAGGAG 3 cut(s) 22, 35, 91
BseXI GCAGC 2 cut(s) 445, 967
BseYI CCCAGC 1 cut(s) 404
Bsh1236I CGCG 7 cut(s) 109, 127, 216, 243, 245, 247, 305
Bsh1285I CGRYCG 1 cut(s) 1003
BshFI GGCC 4 cut(s) 293, 346, 493, 1020
BshNI GGYRCC 2 cut(s) 386, 467
BsiEI CGRYCG 1 cut(s) 1003
BsiHKAI GWGCWC 1 cut(s) 616
BsiHKCI CYCGRG 2 cut(s) 200, 488
BsiSI CCGG 5 cut(s) 185, 348, 489, 494, 513
BslFI GGGAC 1 cut(s) 872
BslI CCNNNNNNNGG 3 cut(s) 353, 1103, 1147
BsmAI GTCTC 6 cut(s) 173, 182, 197, 578, 787, 1060
BsmBI CGTCTC 3 cut(s) 173, 182, 787
BsmFI GGGAC 1 cut(s) 872
BsnI GGCC 4 cut(s) 293, 346, 493, 1020
Bso31I GGTCTC 1 cut(s) 578
BsoBI CYCGRG 2 cut(s) 200, 488
Bsp1286I GDGCHC 1 cut(s) 616
Bsp143I GATC 5 cut(s) 478, 724, 823, 838, 1000
BspANI GGCC 4 cut(s) 293, 346, 493, 1020
BspCNI CTCAG 2 cut(s) 35, 436
BspFNI CGCG 7 cut(s) 109, 127, 216, 243, 245, 247, 305
BspLI GGNNCC 4 cut(s) 388, 469, 498, 888
BspPI GGATC 1 cut(s) 719
BspT107I GGYRCC 2 cut(s) 386, 467
BspTNI GGTCTC 1 cut(s) 578
BsrDI GCAATG 2 cut(s) 557, 629
BsrFI RCCGGY 1 cut(s) 493
BssAI RCCGGY 1 cut(s) 493
BssECI CCNNGG 5 cut(s) 303, 462, 488, 617, 740
BssHII GCGCGC 1 cut(s) 243
BssMI GATC 5 cut(s) 478, 724, 823, 838, 1000
BssNI GRCGYC 5 cut(s) 182, 312, 387, 468, 653
BssT1I CCWWGG 2 cut(s) 462, 617
Bst2UI CCWGG 1 cut(s) 609
Bst4CI ACNGT 1 cut(s) 283
Bst6I CTCTTC 3 cut(s) 73, 1065, 1095
BstACI GRCGYC 5 cut(s) 182, 312, 387, 468, 653
BstAPI GCANNNNNTGC 1 cut(s) 961
BstBAI YACGTR 1 cut(s) 898
BstC8I GCNNGC 3 cut(s) 245, 657, 1135
BstDEI CTNAG 2 cut(s) 22, 444
BstDSI CCRYGG 1 cut(s) 303
BstF5I GGATG 3 cut(s) 532, 682, 1137
BstFNI CGCG 7 cut(s) 109, 127, 216, 243, 245, 247, 305
BstH2I RGCGCY 2 cut(s) 390, 471
BstHHI GCGC 9 cut(s) 111, 129, 164, 245, 247, 274, 389, 470, 553
BstKTI GATC 5 cut(s) 481, 727, 826, 841, 1003
BstMAI GTCTC 6 cut(s) 173, 182, 197, 578, 787, 1060
BstMBI GATC 5 cut(s) 478, 724, 823, 838, 1000
BstMCI CGRYCG 1 cut(s) 1003
BstMWI GCNNNNNNNGC 9 cut(s) 115, 124, 299, 357, 455, 467, 559, 611, 961
BstNI CCWGG 1 cut(s) 609
BstPAI GACNNNNGTC 1 cut(s) 236
BstSCI CCNGG 4 cut(s) 346, 487, 488, 607
BstSNI TACGTA 1 cut(s) 898
BstUI CGCG 7 cut(s) 109, 127, 216, 243, 245, 247, 305
BstV1I GCAGC 2 cut(s) 445, 967
BstXI CCANNNNNNTGG 2 cut(s) 883, 1152
Bsu36I CCTNAGG 1 cut(s) 444
BsuI GTATCC 1 cut(s) 916
BsuRI GGCC 4 cut(s) 293, 346, 493, 1020
BtgI CCRYGG 1 cut(s) 303
BtgZI GCGATG 1 cut(s) 280
BtrI CACGTC 1 cut(s) 334
BtsCI GGATG 3 cut(s) 532, 682, 1137
BtsIMutI CAGTG 3 cut(s) 279, 864, 1064
Cac8I GCNNGC 3 cut(s) 245, 657, 1135
CfoI GCGC 9 cut(s) 111, 129, 164, 245, 247, 274, 389, 470, 553
Cfr10I RCCGGY 1 cut(s) 493
Cfr13I GGNCC 3 cut(s) 345, 491, 886
Cfr42I CCGCGG 1 cut(s) 306
Cfr9I CCCGGG 1 cut(s) 488
CseI GACGC 3 cut(s) 190, 301, 661
Csp6I GTAC 1 cut(s) 899
CviAII CATG 1 cut(s) 951
CviQI GTAC 1 cut(s) 899
DdeI CTNAG 2 cut(s) 22, 444
DinI GGCGCC 2 cut(s) 388, 469
DpnI GATC 5 cut(s) 480, 726, 825, 840, 1002
DpnII GATC 5 cut(s) 478, 724, 823, 838, 1000
DraIII CACNNNGTG 1 cut(s) 285
DriI GACNNNNNGTC 2 cut(s) 337, 1032
Eam1104I CTCTTC 3 cut(s) 73, 1065, 1095
Eam1105I GACNNNNNGTC 2 cut(s) 337, 1032
EarI CTCTTC 3 cut(s) 73, 1065, 1095
EciI GGCGGA 1 cut(s) 122
Eco105I TACGTA 1 cut(s) 898
Eco130I CCWWGG 2 cut(s) 462, 617
Eco31I GGTCTC 1 cut(s) 578
Eco47I GGWCC 1 cut(s) 886
Eco81I CCTNAGG 1 cut(s) 444
Eco88I CYCGRG 2 cut(s) 200, 488
EcoRII CCWGG 1 cut(s) 607
EcoT14I CCWWGG 2 cut(s) 462, 617
EcoT22I ATGCAT 1 cut(s) 534
EgeI GGCGCC 2 cut(s) 388, 469
EheI GGCGCC 2 cut(s) 388, 469
ErhI CCWWGG 2 cut(s) 462, 617
Esp3I CGTCTC 3 cut(s) 173, 182, 787
FaeI CATG 1 cut(s) 954
FaqI GGGAC 1 cut(s) 872
FatI CATG 1 cut(s) 950
FauI CCCGC 1 cut(s) 353
FbaI TGATCA 2 cut(s) 478, 838
FokI GGATG 3 cut(s) 519, 689, 1124
FspBI CTAG 2 cut(s) 618, 1127
GlaI GCGC 9 cut(s) 110, 128, 163, 244, 246, 273, 388, 469, 552
GsaI CCCAGC 1 cut(s) 408
GsuI CTGGAG 1 cut(s) 630
HaeII RGCGCY 2 cut(s) 390, 471
HaeIII GGCC 4 cut(s) 293, 346, 493, 1020
HapII CCGG 5 cut(s) 185, 348, 489, 494, 513
HgaI GACGC 3 cut(s) 190, 301, 661
HhaI GCGC 9 cut(s) 111, 129, 164, 245, 247, 274, 389, 470, 553
Hin1I GRCGYC 5 cut(s) 182, 312, 387, 468, 653
Hin1II CATG 1 cut(s) 954
Hin6I GCGC 9 cut(s) 109, 127, 162, 243, 245, 272, 387, 468, 551
HinP1I GCGC 9 cut(s) 109, 127, 162, 243, 245, 272, 387, 468, 551
HinfI GANTC 3 cut(s) 218, 372, 1038
HpaII CCGG 5 cut(s) 185, 348, 489, 494, 513
HphI GGTGA 1 cut(s) 31
Hpy166II GTNNAC 1 cut(s) 997
Hpy188I TCNGA 1 cut(s) 702
Hpy188III TCNNGA 8 cut(s) 172, 253, 443, 583, 748, 785, 842, 1042
Hpy8I GTNNAC 1 cut(s) 997
Hpy99I CGWCG 3 cut(s) 226, 229, 317
HpyAV CCTTC 8 cut(s) 46, 82, 103, 130, 169, 394, 742, 913
HpyCH4III ACNGT 1 cut(s) 283
HpyCH4IV ACGT 4 cut(s) 320, 333, 897, 973
HpyCH4V TGCA 4 cut(s) 379, 532, 562, 1137
HpyF10VI GCNNNNNNNGC 9 cut(s) 115, 124, 299, 357, 455, 467, 559, 611, 961
HpyF3I CTNAG 2 cut(s) 22, 444
HpySE526I ACGT 4 cut(s) 320, 333, 897, 973
Hsp92I GRCGYC 5 cut(s) 182, 312, 387, 468, 653
Hsp92II CATG 1 cut(s) 954
HspAI GCGC 9 cut(s) 109, 127, 162, 243, 245, 272, 387, 468, 551
KasI GGCGCC 2 cut(s) 386, 467
Ksp22I TGATCA 2 cut(s) 478, 838
KspI CCGCGG 1 cut(s) 306
Kzo9I GATC 5 cut(s) 478, 724, 823, 838, 1000
LmnI GCTCC 3 cut(s) 598, 610, 611
Lsp1109I GCAGC 2 cut(s) 445, 967
LweI GCATC 8 cut(s) 149, 541, 580, 592, 790, 837, 1120, 1146
MaeI CTAG 2 cut(s) 618, 1127
MaeII ACGT 4 cut(s) 320, 333, 897, 973
MaeIII GTNAC 1 cut(s) 713
MalI GATC 5 cut(s) 480, 726, 825, 840, 1002
MauBI CGCGCGCG 1 cut(s) 243
MboI GATC 5 cut(s) 478, 724, 823, 838, 1000
MboII GAAGA 6 cut(s) 60, 268, 496, 1052, 1112, 1124
MfeI CAATTG 1 cut(s) 959
MhlI GDGCHC 1 cut(s) 616
MluCI AATT 6 cut(s) 516, 568, 719, 903, 959, 988
Mly113I GGCGCC 2 cut(s) 387, 468
MlyI GAGTC 2 cut(s) 227, 381
Mph1103I ATGCAT 1 cut(s) 534
MseI TTAA 4 cut(s) 324, 411, 681, 1159
MslI CAYNNNNRTG 1 cut(s) 786
MspA1I CMGCKG 2 cut(s) 305, 476
MspI CCGG 5 cut(s) 185, 348, 489, 494, 513
MspR9I CCNGG 4 cut(s) 348, 489, 490, 609
MunI CAATTG 1 cut(s) 959
MvaI CCWGG 1 cut(s) 609
MvnI CGCG 7 cut(s) 109, 127, 216, 243, 245, 247, 305
MwoI GCNNNNNNNGC 9 cut(s) 115, 124, 299, 357, 455, 467, 559, 611, 961
NarI GGCGCC 2 cut(s) 387, 468
NciI CCSGG 3 cut(s) 348, 489, 490
NdeII GATC 5 cut(s) 478, 724, 823, 838, 1000
NlaIII CATG 1 cut(s) 954
NlaIV GGNNCC 4 cut(s) 388, 469, 498, 888
NmuCI GTSAC 1 cut(s) 713
NsiI ATGCAT 1 cut(s) 534
PauI GCGCGC 1 cut(s) 243
PcsI WCGNNNNNNNCGW 1 cut(s) 227
PfeI GAWTC 1 cut(s) 1038
PflFI GACNNNGTC 1 cut(s) 311
PfoI TCCNGGA 1 cut(s) 607
Ple19I CGATCG 1 cut(s) 1003
PleI GAGTC 2 cut(s) 226, 380
PluTI GGCGCC 2 cut(s) 390, 471
PpsI GAGTC 2 cut(s) 226, 380
Ppu21I YACGTR 1 cut(s) 898
PshAI GACNNNNGTC 1 cut(s) 236
Psp6I CCWGG 1 cut(s) 607
PspFI CCCAGC 1 cut(s) 404
PspGI CCWGG 1 cut(s) 607
PspN4I GGNNCC 4 cut(s) 388, 469, 498, 888
PspPI GGNCC 3 cut(s) 345, 491, 886
PsyI GACNNNGTC 1 cut(s) 311
PteI GCGCGC 1 cut(s) 243
PvuI CGATCG 1 cut(s) 1003
RsaI GTAC 1 cut(s) 900
RsaNI GTAC 1 cut(s) 899
RseI CAYNNNNRTG 1 cut(s) 786
SacII CCGCGG 1 cut(s) 306
SaqAI TTAA 4 cut(s) 324, 411, 681, 1159
Sau3AI GATC 5 cut(s) 478, 724, 823, 838, 1000
Sau96I GGNCC 3 cut(s) 345, 491, 886
SchI GAGTC 2 cut(s) 227, 381
ScrFI CCNGG 4 cut(s) 348, 489, 490, 609
SduI GDGCHC 1 cut(s) 616
SfaNI GCATC 8 cut(s) 149, 541, 580, 592, 790, 837, 1120, 1146
SfoI GGCGCC 2 cut(s) 388, 469
Sfr303I CCGCGG 1 cut(s) 306
SgrBI CCGCGG 1 cut(s) 306
SinI GGWCC 1 cut(s) 886
SmaI CCCGGG 1 cut(s) 490
SmiMI CAYNNNNRTG 1 cut(s) 786
SnaBI TACGTA 1 cut(s) 898
Sse9I AATT 6 cut(s) 516, 568, 719, 903, 959, 988
SspDI GGCGCC 2 cut(s) 386, 467
SspI AATATT 1 cut(s) 813
SspMI CTAG 2 cut(s) 618, 1127
StyD4I CCNGG 4 cut(s) 346, 487, 488, 607
StyI CCWWGG 2 cut(s) 462, 617
TaaI ACNGT 1 cut(s) 283
TaiI ACGT 4 cut(s) 323, 336, 900, 976
TaqI TCGA 6 cut(s) 171, 252, 341, 582, 1036, 1041
TaqII GACCGA 1 cut(s) 602
TasI AATT 6 cut(s) 516, 568, 719, 903, 959, 988
TauI GCSGC 9 cut(s) 7, 302, 305, 354, 357, 360, 473, 476, 512
TfiI GAWTC 1 cut(s) 1038
Tru1I TTAA 4 cut(s) 324, 411, 681, 1159
Tru9I TTAA 4 cut(s) 324, 411, 681, 1159
TscAI CASTG 3 cut(s) 286, 871, 1071
TseFI GTSAC 1 cut(s) 713
TseI GCWGC 2 cut(s) 458, 955
Tsp45I GTSAC 1 cut(s) 713
TspDTI ATGAA 1 cut(s) 680
TspGWI ACGGA 5 cut(s) 206, 226, 489, 717, 950
TspMI CCCGGG 1 cut(s) 488
TspRI CASTG 3 cut(s) 286, 871, 1071
Tth111I GACNNNGTC 1 cut(s) 311
VpaK11BI GGWCC 1 cut(s) 886
XapI RAATTY 1 cut(s) 719
XcmI CCANNNNNNNNNTGG 1 cut(s) 605
XmaI CCCGGG 1 cut(s) 488
XmaJI CCTAGG 1 cut(s) 617
XspI CTAG 2 cut(s) 618, 1127
Zsp2I ATGCAT 1 cut(s) 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.