AT1G03550

Probably involved in membrane trafficking

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005596
Physical Location & Seq
Reverse (-)
885623 .. 887956
2334 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G03550.6

Sequence Viewer

Length: 852 bp
ATGGCACGACACGATCCTAATCCATTTGCTGATGAAGAGATCAATCCTTTTGCGAATCATACAAGTGTTCCTCCTGCGAGCAACTCTTATCTCAAGCCACTTCCACCGGAACCTTATGATCGTGGTGCAACCGTTGATATCCCTTTGGATTCTGGCAATGATCTTCGAGCTAAAGAGATGGAACTCCAGGCCAAGGAGAACGAATTGAAGCGGAAAGAGCAGGAGCTTAAAAGAAGAGAGGACGCAATAGCACGAACTGGAGTTGTCATTGAAGAGAAGAATTGGCCGGAGTTTTTTCCTTTAATTCATCATGACATTCCAAATGAGATTCCAATTCATTTACAGAAGATCCAATACGTCGCATTCACCACATTGTTAGGGTTAGTAGGATGTCTCTTGTGGAATATTGTGGCAGTAACTGTAGCTTGGATCAAAGGAGAAGGTCCCACTATATGGCTTCTCTCAATAATTTACTTCCTTGCTGGGGTACCTGGAGCGTACGTCTTATGGTACCGTCCTCTTTACCGAGCTACAAGAACTGATAGTGCCCTGAAGTTTGGAGCTTTTTTCTTTTTTTACGTGTTCCACATCGCGTTCTGCGGCTTTGCTGCAGTTGCTCCACCAGTCATCTTCCAAGGAAAGTCTCTCACAGGTTTCTTGCCAGCAATCGAGCTTCTAACTACTAATGCTGCAGTCGGGATAATGTATTTCATTGGTGCTGGGTTCTTCTGCATCGAAACACTTCTCAATATCTGGGTCATTCAGCAAGTATATGCATACTTCCGAGGGAGTGGCAAAGCTGCAGAGATGAAGCGTGAAGCTACAAAATCGACCTTGATGCGTGCACTATGA

Protein Analysis

283

Amino Acids

31.87

Weight (kDa)

6.6

Isoelectric Point (pI)

43.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAMP PF04144 92 - 208 6.7e-43 SCAMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 487, 510
AccB1I GGYRCC 2 cut(s) 487, 510
AccB7I CCANNNNNTGG 1 cut(s) 453
AccII CGCG 1 cut(s) 593
AciI CCGC 2 cut(s) 211, 600
AclWI GGATC 3 cut(s) 8, 343, 437
AcoI YGGCCR 1 cut(s) 284
AcuI CTGAAG 1 cut(s) 572
AfaI GTAC 3 cut(s) 489, 500, 512
AfiI CCNNNNNNNGG 3 cut(s) 193, 453, 484
AflIII ACRYGT 1 cut(s) 579
AgsI TTSAA 2 cut(s) 208, 272
AjnI CCWGG 2 cut(s) 186, 490
AluBI AGCT 8 cut(s) 170, 226, 425, 530, 563, 673, 800, 821
AluI AGCT 8 cut(s) 170, 226, 425, 530, 563, 673, 800, 821
Alw21I GWGCWC 1 cut(s) 847
Alw26I GTCTC 2 cut(s) 398, 648
Alw44I GTGCAC 1 cut(s) 843
AlwI GGATC 3 cut(s) 8, 343, 437
AlwNI CAGNNNCTG 1 cut(s) 419
AoxI GGCC 2 cut(s) 189, 284
ApaLI GTGCAC 1 cut(s) 843
ApeKI GCWGC 3 cut(s) 608, 689, 800
Asp700I GAANNNNTTC 1 cut(s) 741
Asp718I GGTACC 2 cut(s) 487, 510
AspS9I GGNCC 1 cut(s) 443
AsuHPI GGTGA 1 cut(s) 358
AvaII GGWCC 1 cut(s) 443
BaeGI GKGCMC 2 cut(s) 550, 847
BanI GGYRCC 2 cut(s) 487, 510
BarI GAAGNNNNNNTAC 2 cut(s) 338, 370
Bbv12I GWGCWC 1 cut(s) 847
BbvI GCAGC 3 cut(s) 595, 676, 787
BccI CCATC 1 cut(s) 172
BcgI CGANNNNNNTGC 1 cut(s) 820
BciT130I CCWGG 2 cut(s) 188, 492
BcoDI GTCTC 2 cut(s) 398, 648
BfmI CTRYAG 4 cut(s) 420, 609, 690, 801
BisI GCNGC 4 cut(s) 601, 609, 690, 801
BlsI GCNGC 4 cut(s) 602, 610, 691, 802
Bme1390I CCNGG 2 cut(s) 188, 492
Bme18I GGWCC 1 cut(s) 443
BmgT120I GGNCC 1 cut(s) 443
BmiI GGNNCC 4 cut(s) 111, 445, 489, 512
BmrFI CCNGG 2 cut(s) 188, 492
BmsI GCATC 2 cut(s) 741, 828
BpmI CTGGAG 3 cut(s) 170, 279, 513
BpuEI CTTGAG 1 cut(s) 77
BsaAI YACGTR 1 cut(s) 580
BsaBI GATNNNNATC 1 cut(s) 18
BsaJI CCNNGG 3 cut(s) 192, 634, 784
BsaWI WCCGGW 1 cut(s) 106
BsaXI ACNNNNNCTCC 2 cut(s) 486, 516
Bsc4I CCNNNNNNNGG 3 cut(s) 193, 453, 484
Bse1I ACTGG 2 cut(s) 262, 623
Bse3DI GCAATG 1 cut(s) 163
Bse8I GATNNNNATC 1 cut(s) 18
BseBI CCWGG 2 cut(s) 188, 492
BseDI CCNNGG 3 cut(s) 192, 634, 784
BseGI GGATG 1 cut(s) 395
BseJI GATNNNNATC 1 cut(s) 18
BseLI CCNNNNNNNGG 3 cut(s) 193, 453, 484
BseMI GCAATG 1 cut(s) 163
BseNI ACTGG 2 cut(s) 262, 623
BseSI GKGCMC 2 cut(s) 550, 847
BseXI GCAGC 3 cut(s) 595, 676, 787
BseYI CCCAGC 2 cut(s) 482, 719
Bsh1236I CGCG 1 cut(s) 593
BshFI GGCC 2 cut(s) 191, 286
BshNI GGYRCC 2 cut(s) 487, 510
BsiHKAI GWGCWC 1 cut(s) 847
BsiSI CCGG 2 cut(s) 107, 287
BsiWI CGTACG 1 cut(s) 498
BslFI GGGAC 1 cut(s) 429
BslI CCNNNNNNNGG 3 cut(s) 193, 453, 484
BsmAI GTCTC 2 cut(s) 398, 648
BsmFI GGGAC 1 cut(s) 429
BsmI GAATGC 1 cut(s) 362
BsnI GGCC 2 cut(s) 191, 286
Bsp1286I GDGCHC 2 cut(s) 550, 847
Bsp143I GATC 6 cut(s) 13, 39, 118, 160, 348, 429
BspACI CCGC 2 cut(s) 211, 600
BspANI GGCC 2 cut(s) 191, 286
BspFNI CGCG 1 cut(s) 593
BspHI TCATGA 1 cut(s) 310
BspLI GGNNCC 4 cut(s) 111, 445, 489, 512
BspMAI CTGCAG 3 cut(s) 613, 694, 805
BspPI GGATC 3 cut(s) 8, 343, 437
BspT107I GGYRCC 2 cut(s) 487, 510
BsrDI GCAATG 1 cut(s) 163
BsrI ACTGG 2 cut(s) 262, 623
BssECI CCNNGG 3 cut(s) 192, 634, 784
BssMI GATC 6 cut(s) 13, 39, 118, 160, 348, 429
BssT1I CCWWGG 2 cut(s) 192, 634
Bst2UI CCWGG 2 cut(s) 188, 492
Bst4CI ACNGT 3 cut(s) 133, 421, 515
Bst6I CTCTTC 3 cut(s) 30, 229, 267
BstBAI YACGTR 1 cut(s) 580
BstC8I GCNNGC 3 cut(s) 79, 663, 843
BstF5I GGATG 1 cut(s) 395
BstFNI CGCG 1 cut(s) 593
BstKTI GATC 6 cut(s) 16, 42, 121, 163, 351, 432
BstMAI GTCTC 2 cut(s) 398, 648
BstMBI GATC 6 cut(s) 13, 39, 118, 160, 348, 429
BstMWI GCNNNNNNNGC 2 cut(s) 217, 614
BstNI CCWGG 2 cut(s) 188, 492
BstSCI CCNGG 2 cut(s) 186, 490
BstSFI CTRYAG 4 cut(s) 420, 609, 690, 801
BstSLI GKGCMC 2 cut(s) 550, 847
BstUI CGCG 1 cut(s) 593
BstV1I GCAGC 3 cut(s) 595, 676, 787
BstX2I RGATCY 1 cut(s) 348
BstYI RGATCY 1 cut(s) 348
BsuRI GGCC 2 cut(s) 191, 286
BtgZI GCGATG 1 cut(s) 574
BtsCI GGATG 1 cut(s) 395
Cac8I GCNNGC 3 cut(s) 79, 663, 843
CaiI CAGNNNCTG 1 cut(s) 419
CciI TCATGA 1 cut(s) 310
Cfr13I GGNCC 1 cut(s) 443
CseI GACGC 1 cut(s) 251
Csp6I GTAC 3 cut(s) 488, 499, 511
CviAII CATG 1 cut(s) 311
CviQI GTAC 3 cut(s) 488, 499, 511
DpnI GATC 6 cut(s) 15, 41, 120, 162, 350, 431
DpnII GATC 6 cut(s) 13, 39, 118, 160, 348, 429
EaeI YGGCCR 1 cut(s) 284
Eam1104I CTCTTC 3 cut(s) 30, 229, 267
EarI CTCTTC 3 cut(s) 30, 229, 267
Eco130I CCWWGG 2 cut(s) 192, 634
Eco32I GATATC 1 cut(s) 139
Eco47I GGWCC 1 cut(s) 443
Eco57I CTGAAG 1 cut(s) 572
EcoO109I RGGNCCY 1 cut(s) 443
EcoRII CCWGG 2 cut(s) 186, 490
EcoRV GATATC 1 cut(s) 139
EcoT14I CCWWGG 2 cut(s) 192, 634
EcoT22I ATGCAT 1 cut(s) 778
ErhI CCWWGG 2 cut(s) 192, 634
FaeI CATG 1 cut(s) 314
FaqI GGGAC 1 cut(s) 429
FatI CATG 1 cut(s) 310
Fnu4HI GCNGC 4 cut(s) 601, 609, 690, 801
FokI GGATG 1 cut(s) 402
Fsp4HI GCNGC 4 cut(s) 601, 609, 690, 801
GluI GCNGC 4 cut(s) 601, 609, 690, 801
GsaI CCCAGC 2 cut(s) 486, 723
GsuI CTGGAG 3 cut(s) 170, 279, 513
HaeIII GGCC 2 cut(s) 191, 286
HapII CCGG 2 cut(s) 107, 287
HgaI GACGC 1 cut(s) 251
Hin1II CATG 1 cut(s) 314
HinfI GANTC 3 cut(s) 55, 149, 328
HpaII CCGG 2 cut(s) 107, 287
HphI GGTGA 1 cut(s) 358
Hpy166II GTNNAC 1 cut(s) 845
Hpy188I TCNGA 1 cut(s) 785
Hpy188III TCNNGA 2 cut(s) 311, 697
Hpy8I GTNNAC 1 cut(s) 845
Hpy99I CGWCG 1 cut(s) 362
HpyAV CCTTC 1 cut(s) 434
HpyCH4III ACNGT 3 cut(s) 133, 421, 515
HpyCH4IV ACGT 3 cut(s) 357, 501, 579
HpyCH4V TGCA 7 cut(s) 128, 611, 692, 732, 776, 803, 845
HpyF10VI GCNNNNNNNGC 2 cut(s) 217, 614
HpySE526I ACGT 3 cut(s) 357, 501, 579
Hsp92II CATG 1 cut(s) 314
KpnI GGTACC 2 cut(s) 491, 514
Kzo9I GATC 6 cut(s) 13, 39, 118, 160, 348, 429
LmnI GCTCC 4 cut(s) 223, 494, 560, 622
Lsp1109I GCAGC 3 cut(s) 595, 676, 787
LweI GCATC 2 cut(s) 741, 828
MaeII ACGT 3 cut(s) 357, 501, 579
MaeIII GTNAC 1 cut(s) 415
MalI GATC 6 cut(s) 15, 41, 120, 162, 350, 431
MboI GATC 6 cut(s) 13, 39, 118, 160, 348, 429
MboII GAAGA 8 cut(s) 47, 155, 246, 284, 289, 358, 622, 718
MflI RGATCY 1 cut(s) 348
MhlI GDGCHC 2 cut(s) 550, 847
MluCI AATT 5 cut(s) 203, 280, 303, 333, 468
MnlI CCTC 4 cut(s) 81, 232, 528, 779
Mph1103I ATGCAT 1 cut(s) 778
MroXI GAANNNNTTC 1 cut(s) 741
MseI TTAA 2 cut(s) 228, 302
MslI CAYNNNNRTG 1 cut(s) 63
MspI CCGG 2 cut(s) 107, 287
MspR9I CCNGG 2 cut(s) 188, 492
Mva1269I GAATGC 1 cut(s) 362
MvaI CCWGG 2 cut(s) 188, 492
MvnI CGCG 1 cut(s) 593
MwoI GCNNNNNNNGC 2 cut(s) 217, 614
NdeII GATC 6 cut(s) 13, 39, 118, 160, 348, 429
NlaIII CATG 1 cut(s) 314
NlaIV GGNNCC 4 cut(s) 111, 445, 489, 512
NsiI ATGCAT 1 cut(s) 778
PagI TCATGA 1 cut(s) 310
PctI GAATGC 1 cut(s) 362
PdmI GAANNNNTTC 1 cut(s) 741
PfeI GAWTC 3 cut(s) 55, 149, 328
Pfl23II CGTACG 1 cut(s) 498
PflMI CCANNNNNTGG 1 cut(s) 453
PkrI GCNGC 4 cut(s) 602, 610, 691, 802
Ppu21I YACGTR 1 cut(s) 580
PpuMI RGGWCCY 1 cut(s) 443
Psp5II RGGWCCY 1 cut(s) 443
Psp6I CCWGG 2 cut(s) 186, 490
PspFI CCCAGC 2 cut(s) 482, 719
PspGI CCWGG 2 cut(s) 186, 490
PspLI CGTACG 1 cut(s) 498
PspN4I GGNNCC 4 cut(s) 111, 445, 489, 512
PspPI GGNCC 1 cut(s) 443
PspPPI RGGWCCY 1 cut(s) 443
PstI CTGCAG 3 cut(s) 613, 694, 805
PstNI CAGNNNCTG 1 cut(s) 419
PsuI RGATCY 1 cut(s) 348
RsaI GTAC 3 cut(s) 489, 500, 512
RsaNI GTAC 3 cut(s) 488, 499, 511
RseI CAYNNNNRTG 1 cut(s) 63
SaqAI TTAA 2 cut(s) 228, 302
SatI GCNGC 4 cut(s) 601, 609, 690, 801
Sau3AI GATC 6 cut(s) 13, 39, 118, 160, 348, 429
Sau96I GGNCC 1 cut(s) 443
ScrFI CCNGG 2 cut(s) 188, 492
SduI GDGCHC 2 cut(s) 550, 847
SfaNI GCATC 2 cut(s) 741, 828
SfcI CTRYAG 4 cut(s) 420, 609, 690, 801
SinI GGWCC 1 cut(s) 443
SmiMI CAYNNNNRTG 1 cut(s) 63
SmlI CTYRAG 1 cut(s) 92
SmoI CTYRAG 1 cut(s) 92
Sse9I AATT 5 cut(s) 203, 280, 303, 333, 468
SsiI CCGC 2 cut(s) 211, 600
SspI AATATT 1 cut(s) 406
StyD4I CCNGG 2 cut(s) 186, 490
StyI CCWWGG 2 cut(s) 192, 634
TaaI ACNGT 3 cut(s) 133, 421, 515
TaiI ACGT 3 cut(s) 360, 504, 582
TaqI TCGA 4 cut(s) 166, 669, 735, 830
TasI AATT 5 cut(s) 203, 280, 303, 333, 468
TauI GCSGC 1 cut(s) 603
TfiI GAWTC 3 cut(s) 55, 149, 328
Tru1I TTAA 2 cut(s) 228, 302
Tru9I TTAA 2 cut(s) 228, 302
TseI GCWGC 3 cut(s) 608, 689, 800
TspDTI ATGAA 5 cut(s) 48, 296, 326, 700, 824
Van91I CCANNNNNTGG 1 cut(s) 453
VneI GTGCAC 1 cut(s) 843
VpaK11BI GGWCC 1 cut(s) 443
XmnI GAANNNNTTC 1 cut(s) 741
Zsp2I ATGCAT 1 cut(s) 778
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.