MD17G1081500.v1.1

secretory carrier-associated membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0005596
Physical Location & Seq
Forward (+)
6809424 .. 6812914
3491 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1081500.v1.1.491

Sequence Viewer

Length: 852 bp
ATGAATCGATACGATTCGAACCCTTTCGCCGACGAGGAGGTCAATCCCTTTGCGAATGTAGGTGGAGGTGTTGCCCCTGCAAATCCAAGGCTTTCGCCCCTTCCTCCGGAACCCTATGATCGCAATGCAACAATTGATATTCCTCTTGACAATTCAAAGAGTATACAAGCAAAGGAGAAGGAACTCAAAGCTAAAGAGGCTGAATTGAAAAAGAGGGAACAGGAACTAAAGCGCAAAGAAGATGCCATAAAACAAGCTGGAGTTGTTATAGAGGAGAAGAACTGGCCTCCATTTTTCCCCATCATCCATCATGACATTGCAAATGAAATACCAATCCATCTACAGAGGATCCAGTATGTTGCGTTCACAACATATTTGGGTTTGATTGTGTGTCTCCTGTGGAATATTCTGGCAGTCACCGTAGCCTGGATCCAAGGAGAAGGTGTAATAATTTGGCTTCTGGCCATAATCTACTTACTAGCAACTGTTCCTGTAGCCTATTTCGTCTGGTATCGCCCTCTTTATCGTGCTATGAGGACTGATAGTGCTCTGAGTTTTGCTGGGTTTTTCTTGTCTTATCTGCTTCATATTGCGTTTTGCATTTACGCTGCAATTGCTCCTCCAATCTTCTTAAAAGGGAAATCTATCACGGGTATTTTGCCTGCAATACAACTGATGAGTTACAACGTTTCGGTTGGGATACTCTACTTCATTGGATTTGCATTGTACGTCTTGGAAACGCTTCTCAGCATTTGGGTTATTCAGCAAGTATACACGTATTTCCGAGGCAGCGGAAAGGCAGAAGAGGCGAAGCGGGAGGCTGCACGAACCACCATGATGGCAGCAATATGA

Protein Analysis

284

Amino Acids

31.94

Weight (kDa)

7.71

Isoelectric Point (pI)

43.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAMP PF04144 92 - 263 1.2e-54 SCAMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 38
AccI GTMKAC 2 cut(s) 163, 771
AccIII TCCGGA 1 cut(s) 106
AciI CCGC 2 cut(s) 792, 814
AclI AACGTT 1 cut(s) 687
AclWI GGATC 4 cut(s) 343, 356, 424, 437
AcoI YGGCCR 1 cut(s) 462
AfaI GTAC 1 cut(s) 728
AfiI CCNNNNNNNGG 2 cut(s) 106, 426
AflIII ACRYGT 1 cut(s) 774
AgsI TTSAA 2 cut(s) 156, 208
AjnI CCWGG 1 cut(s) 425
AluBI AGCT 2 cut(s) 191, 257
AluI AGCT 2 cut(s) 191, 257
Alw21I GWGCWC 1 cut(s) 550
Alw26I GTCTC 1 cut(s) 398
AlwI GGATC 4 cut(s) 343, 356, 424, 437
Aor13HI TCCGGA 1 cut(s) 106
AoxI GGCC 2 cut(s) 284, 462
ApeKI GCWGC 4 cut(s) 608, 789, 821, 842
Asp700I GAANNNNTTC 2 cut(s) 23, 741
AspLEI GCGC 1 cut(s) 234
AsuHPI GGTGA 1 cut(s) 409
AsuII TTCGAA 1 cut(s) 17
BalI TGGCCA 1 cut(s) 464
BamHI GGATCC 2 cut(s) 348, 429
Bbv12I GWGCWC 1 cut(s) 550
BbvI GCAGC 3 cut(s) 595, 801, 808
BccI CCATC 4 cut(s) 308, 315, 345, 832
BciT130I CCWGG 1 cut(s) 427
BciVI GTATCC 1 cut(s) 693
BcoDI GTCTC 1 cut(s) 398
BfaI CTAG 1 cut(s) 479
BfmI CTRYAG 2 cut(s) 341, 492
BfuI GTATCC 1 cut(s) 693
BisI GCNGC 4 cut(s) 609, 790, 822, 843
BlsI GCNGC 4 cut(s) 610, 791, 823, 844
Bme1390I CCNGG 1 cut(s) 427
BmiI GGNNCC 3 cut(s) 111, 350, 431
BmrFI CCNGG 1 cut(s) 427
BmsI GCATC 1 cut(s) 232
BpmI CTGGAG 1 cut(s) 279
Bpu14I TTCGAA 1 cut(s) 17
Bsa29I ATCGAT 1 cut(s) 7
BsaAI YACGTR 1 cut(s) 777
BsaJI CCNNGG 3 cut(s) 86, 433, 784
BsaWI WCCGGW 1 cut(s) 106
BsaXI ACNNNNNCTCC 2 cut(s) 429, 459
Bsc4I CCNNNNNNNGG 2 cut(s) 106, 426
Bse1I ACTGG 2 cut(s) 287, 352
Bse3DI GCAATG 2 cut(s) 130, 315
BseAI TCCGGA 1 cut(s) 106
BseBI CCWGG 1 cut(s) 427
BseCI ATCGAT 1 cut(s) 7
BseDI CCNNGG 3 cut(s) 86, 433, 784
BseGI GGATG 1 cut(s) 303
BseLI CCNNNNNNNGG 2 cut(s) 106, 426
BseMI GCAATG 2 cut(s) 130, 315
BseMII CTCAG 2 cut(s) 542, 760
BseNI ACTGG 2 cut(s) 287, 352
BseRI GAGGAG 3 cut(s) 50, 287, 609
BseXI GCAGC 3 cut(s) 595, 801, 808
BseYI CCCAGC 1 cut(s) 560
BsgI GTGCAG 1 cut(s) 807
BshFI GGCC 2 cut(s) 286, 464
BshVI ATCGAT 1 cut(s) 7
BsiHKAI GWGCWC 1 cut(s) 550
BsiSI CCGG 1 cut(s) 107
BslI CCNNNNNNNGG 2 cut(s) 106, 426
BsmAI GTCTC 1 cut(s) 398
BsnI GGCC 2 cut(s) 286, 464
Bsp119I TTCGAA 1 cut(s) 17
Bsp1286I GDGCHC 1 cut(s) 550
Bsp13I TCCGGA 1 cut(s) 106
Bsp143I GATC 3 cut(s) 118, 348, 429
BspACI CCGC 2 cut(s) 792, 814
BspANI GGCC 2 cut(s) 286, 464
BspCNI CTCAG 2 cut(s) 543, 759
BspDI ATCGAT 1 cut(s) 7
BspEI TCCGGA 1 cut(s) 106
BspHI TCATGA 1 cut(s) 310
BspLI GGNNCC 3 cut(s) 111, 350, 431
BspPI GGATC 4 cut(s) 343, 356, 424, 437
BspT104I TTCGAA 1 cut(s) 17
BsrDI GCAATG 2 cut(s) 130, 315
BsrI ACTGG 2 cut(s) 287, 352
BssECI CCNNGG 3 cut(s) 86, 433, 784
BssMI GATC 3 cut(s) 118, 348, 429
BssNAI GTATAC 2 cut(s) 164, 772
BssT1I CCWWGG 2 cut(s) 86, 433
Bst1107I GTATAC 2 cut(s) 164, 772
Bst2UI CCWGG 1 cut(s) 427
Bst4CI ACNGT 2 cut(s) 421, 487
Bst6I CTCTTC 1 cut(s) 798
BstBAI YACGTR 1 cut(s) 777
BstBI TTCGAA 1 cut(s) 17
BstC8I GCNNGC 1 cut(s) 663
BstDEI CTNAG 2 cut(s) 551, 746
BstF5I GGATG 1 cut(s) 303
BstHHI GCGC 1 cut(s) 234
BstKTI GATC 3 cut(s) 121, 351, 432
BstMAI GTCTC 1 cut(s) 398
BstMBI GATC 3 cut(s) 118, 348, 429
BstMWI GCNNNNNNNGC 3 cut(s) 197, 614, 806
BstNI CCWGG 1 cut(s) 427
BstSCI CCNGG 1 cut(s) 425
BstSFI CTRYAG 2 cut(s) 341, 492
BstV1I GCAGC 3 cut(s) 595, 801, 808
BstX2I RGATCY 2 cut(s) 348, 429
BstXI CCANNNNNNTGG 1 cut(s) 838
BstYI RGATCY 2 cut(s) 348, 429
BstZ17I GTATAC 2 cut(s) 164, 772
Bsu15I ATCGAT 1 cut(s) 7
BsuI GTATCC 1 cut(s) 693
BsuRI GGCC 2 cut(s) 286, 464
BsuTUI ATCGAT 1 cut(s) 7
BtsCI GGATG 1 cut(s) 303
Cac8I GCNNGC 1 cut(s) 663
CciI TCATGA 1 cut(s) 310
CfoI GCGC 1 cut(s) 234
ClaI ATCGAT 1 cut(s) 7
Csp6I GTAC 1 cut(s) 727
CviAII CATG 2 cut(s) 311, 835
CviQI GTAC 1 cut(s) 727
DdeI CTNAG 2 cut(s) 551, 746
DpnI GATC 3 cut(s) 120, 350, 431
DpnII GATC 3 cut(s) 118, 348, 429
DrdI GACNNNNNNGTC 1 cut(s) 38
DseDI GACNNNNNNGTC 1 cut(s) 38
EaeI YGGCCR 1 cut(s) 462
Eam1104I CTCTTC 1 cut(s) 798
EarI CTCTTC 1 cut(s) 798
Eco130I CCWWGG 2 cut(s) 86, 433
EcoRII CCWGG 1 cut(s) 425
EcoT14I CCWWGG 2 cut(s) 86, 433
ErhI CCWWGG 2 cut(s) 86, 433
FaeI CATG 2 cut(s) 314, 838
FatI CATG 2 cut(s) 310, 834
FauI CCCGC 1 cut(s) 807
FblI GTMKAC 2 cut(s) 163, 771
Fnu4HI GCNGC 4 cut(s) 609, 790, 822, 843
FokI GGATG 1 cut(s) 290
Fsp4HI GCNGC 4 cut(s) 609, 790, 822, 843
FspBI CTAG 1 cut(s) 479
GlaI GCGC 1 cut(s) 233
GluI GCNGC 4 cut(s) 609, 790, 822, 843
GsaI CCCAGC 1 cut(s) 564
GsuI CTGGAG 1 cut(s) 279
HaeIII GGCC 2 cut(s) 286, 464
HapII CCGG 1 cut(s) 107
HhaI GCGC 1 cut(s) 234
Hin1II CATG 2 cut(s) 314, 838
Hin6I GCGC 1 cut(s) 232
HinP1I GCGC 1 cut(s) 232
HinfI GANTC 2 cut(s) 4, 14
HpaII CCGG 1 cut(s) 107
HphI GGTGA 1 cut(s) 409
Hpy166II GTNNAC 3 cut(s) 164, 366, 772
Hpy188I TCNGA 2 cut(s) 552, 785
Hpy188III TCNNGA 3 cut(s) 107, 146, 311
Hpy8I GTNNAC 3 cut(s) 164, 366, 772
Hpy99I CGWCG 1 cut(s) 35
HpyAV CCTTC 3 cut(s) 110, 172, 434
HpyCH4III ACNGT 2 cut(s) 421, 487
HpyCH4IV ACGT 3 cut(s) 687, 729, 776
HpyCH4V TGCA 8 cut(s) 80, 128, 320, 600, 611, 665, 722, 824
HpyF10VI GCNNNNNNNGC 3 cut(s) 197, 614, 806
HpyF3I CTNAG 2 cut(s) 551, 746
HpySE526I ACGT 3 cut(s) 687, 729, 776
Hsp92II CATG 2 cut(s) 314, 838
HspAI GCGC 1 cut(s) 232
Kpn2I TCCGGA 1 cut(s) 106
Kzo9I GATC 3 cut(s) 118, 348, 429
LmnI GCTCC 1 cut(s) 622
Lsp1109I GCAGC 3 cut(s) 595, 801, 808
LweI GCATC 1 cut(s) 232
MaeI CTAG 1 cut(s) 479
MaeII ACGT 3 cut(s) 687, 729, 776
MaeIII GTNAC 2 cut(s) 415, 680
MalI GATC 3 cut(s) 120, 350, 431
MboI GATC 3 cut(s) 118, 348, 429
MboII GAAGA 4 cut(s) 251, 289, 619, 815
MfeI CAATTG 2 cut(s) 132, 612
MflI RGATCY 2 cut(s) 348, 429
MhlI GDGCHC 1 cut(s) 550
MlsI TGGCCA 1 cut(s) 464
MluCI AATT 5 cut(s) 132, 151, 203, 450, 612
MluNI TGGCCA 1 cut(s) 464
Mox20I TGGCCA 1 cut(s) 464
MroI TCCGGA 1 cut(s) 106
MroXI GAANNNNTTC 2 cut(s) 23, 741
MscI TGGCCA 1 cut(s) 464
MseI TTAA 1 cut(s) 632
MslI CAYNNNNRTG 1 cut(s) 836
Msp20I TGGCCA 1 cut(s) 464
MspA1I CMGCKG 1 cut(s) 792
MspI CCGG 1 cut(s) 107
MspR9I CCNGG 1 cut(s) 427
MunI CAATTG 2 cut(s) 132, 612
MvaI CCWGG 1 cut(s) 427
MwoI GCNNNNNNNGC 3 cut(s) 197, 614, 806
NdeII GATC 3 cut(s) 118, 348, 429
NlaIII CATG 2 cut(s) 314, 838
NlaIV GGNNCC 3 cut(s) 111, 350, 431
NmuCI GTSAC 1 cut(s) 415
NspV TTCGAA 1 cut(s) 17
PagI TCATGA 1 cut(s) 310
PdmI GAANNNNTTC 2 cut(s) 23, 741
PfeI GAWTC 2 cut(s) 4, 14
PkrI GCNGC 4 cut(s) 610, 791, 823, 844
Ppu21I YACGTR 1 cut(s) 777
Psp1406I AACGTT 1 cut(s) 687
Psp6I CCWGG 1 cut(s) 425
PspFI CCCAGC 1 cut(s) 560
PspGI CCWGG 1 cut(s) 425
PspN4I GGNNCC 3 cut(s) 111, 350, 431
PsrI GAACNNNNNNTAC 2 cut(s) 347, 379
PsuI RGATCY 2 cut(s) 348, 429
RsaI GTAC 1 cut(s) 728
RsaNI GTAC 1 cut(s) 727
RseI CAYNNNNRTG 1 cut(s) 836
SaqAI TTAA 1 cut(s) 632
SatI GCNGC 4 cut(s) 609, 790, 822, 843
Sau3AI GATC 3 cut(s) 118, 348, 429
ScrFI CCNGG 1 cut(s) 427
SduI GDGCHC 1 cut(s) 550
SetI ASST 9 cut(s) 42, 64, 70, 193, 259, 445, 690, 732, 779
SfaNI GCATC 1 cut(s) 232
SfcI CTRYAG 2 cut(s) 341, 492
SfuI TTCGAA 1 cut(s) 17
SmiMI CAYNNNNRTG 1 cut(s) 836
Sse9I AATT 5 cut(s) 132, 151, 203, 450, 612
SsiI CCGC 2 cut(s) 792, 814
SspI AATATT 1 cut(s) 406
SspMI CTAG 1 cut(s) 479
StyD4I CCNGG 1 cut(s) 425
StyI CCWWGG 2 cut(s) 86, 433
TaaI ACNGT 2 cut(s) 421, 487
TaiI ACGT 3 cut(s) 690, 732, 779
TaqI TCGA 2 cut(s) 7, 17
TasI AATT 5 cut(s) 132, 151, 203, 450, 612
TfiI GAWTC 2 cut(s) 4, 14
Tru1I TTAA 1 cut(s) 632
Tru9I TTAA 1 cut(s) 632
TseFI GTSAC 1 cut(s) 415
TseI GCWGC 4 cut(s) 608, 789, 821, 842
Tsp45I GTSAC 1 cut(s) 415
TspDTI ATGAA 4 cut(s) 17, 339, 575, 700
XmiI GTMKAC 2 cut(s) 163, 771
XmnI GAANNNNTTC 2 cut(s) 23, 741
XspI CTAG 1 cut(s) 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.