AT1G05577

Domain of unknown function (DUF966)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0014395
Physical Location & Seq
Reverse (-)
1662352 .. 1664398
2047 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G05577.1

Sequence Viewer

Length: 1119 bp
ATGGAAAGTAATGGTGGAGGAGGAGAAGTAAGAAGAGTGAATTTAGTTTATTTCCTTAGCCGTTCGGGTCACGTTGACCATCCTCATCTCTTGCGTGTTCATCATCTCTCTCGTAATGGAGTCTTTCTCCGAGATGTGAAGAAATGGTTAGCAGACGCACGAGGCGATGCCATGCCTGATGCATTTTCTTGGTCTTGTAAGAGGAGGTATAAGAATGGTTATGTATGGCAAGATTTATTGGACGATGATCTTATCACTCCTATCTCTGATAATGAATATGTCCTCAAAGGATCTGAGATTCTTCTAAGCTCTCCAAAGGAGGATTATCCTAATGTGGAGAAGAAAGCTTGGGTGACAAGAAACGGTGGAATAGACGCTGAAGAAAAACTCCAGAAGTTGAAACTAACGTCAGAGAAGATCCAAAAAGAGTCTCCGGTCTTCTGCTCACAGAGATCGACGGCAACAACGTCGACAGTCACGGAGGAATCCACCACGAACGAAGAAGGTTTCGTTCTTAAGAAACAAGATCCGAAAACAGTCTCCGGCCAGAGAGACGGTTCGACGGAGAACGGGTCGGGTAACGATGTGGAGTCGGGTCGACCAAGTGTATCGTCTACGACGTCGTCTTCGAGTTATATCAAGAATAAGAGCTATTCGAGCGTACGTGCCTCTCACGTGTTACGGAACTTGATGAAGTGTGGTGGTTTGGACACAAACGACGCCGTATTGGTGCCTTTGAATAAGTCTAGGTCTGGGGCTTTTGGTCCGGCTTGGGAAGATGAACGCAGATACCAATACCACCAACAGCACAATGCTCGAAAAAGCTTCGAAGGAGCATGGAGTGGCATAAAGATGAAAGAGACAATTGAGTTTTGTAAACCAAAGGTTGCTCCAAGCAAGCCTTCCATGGCTCCATTATGCTCGCAATGTGGGAAGTTGTTCAAACCTGAGAAGATGCATTCACACATGAAATTATGTCGAGGGATGAAGAATTCATCGGCGAATAATGATTTGATGACAAGCAATAATACCGTTAAACCGAGGCAGCAACGGTGCAGAAACATTCCTGGAAATCCATTGGGACATCAACGTGTATTGACGACTACTCTCAAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF966

Protein Analysis

372

Amino Acids

41.64

Weight (kDa)

9.39

Isoelectric Point (pI)

47.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOK PF06136 13 - 101 5.2e-34 SOSEKI protein DIX-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014395)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05577
fragaria_vesca FvH4_2g11530
malus_domestica MD07G1001300.v1.1 MD07G1001400.v1.1
prunus_persica Prupe.2G001400_v2.0.a1 Prupe.2G001400_v2.0.a1
pyrus_communis pycom07g00180
rosa_chinensis RchiOBHm_Chr1g0313061
rosa_laevigata RLG00000030824
rosa_multiflora Rmu_sc0001606.1_g000003
rosa_roxburghii Rroxscaffold_4G00332510
rosa_rugosa RorugPtG0000700
rosa_samantha Rh1AG000600 Rh1CG002200 Rh1CG004800 Rh1DG000300
rosa_wichuraiana Rw0G008490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 623
AccB1I GGYRCC 1 cut(s) 730
AccI GTMKAC 3 cut(s) 470, 598, 614
AclWI GGATC 3 cut(s) 298, 412, 521
AcoI YGGCCR 1 cut(s) 544
AcsI RAATTY 2 cut(s) 40, 991
AcuI CTGAAG 1 cut(s) 399
AcvI CACGTG 1 cut(s) 676
AcyI GRCGYC 2 cut(s) 620, 720
AfaI GTAC 1 cut(s) 663
AflII CTTAAG 1 cut(s) 515
AflIII ACRYGT 2 cut(s) 675, 1090
AgsI TTSAA 3 cut(s) 400, 739, 943
AjnI CCWGG 1 cut(s) 1066
AjuI GAANNNNNNNTTGG 2 cut(s) 886, 918
AluBI AGCT 4 cut(s) 309, 347, 651, 825
AluI AGCT 4 cut(s) 309, 347, 651, 825
Alw26I GTCTC 4 cut(s) 435, 544, 546, 854
AlwI GGATC 3 cut(s) 298, 412, 521
AoxI GGCC 1 cut(s) 544
ApeKI GCWGC 1 cut(s) 1045
ApoI RAATTY 2 cut(s) 40, 991
ArsI GACNNNNNNTTYG 2 cut(s) 610, 642
Asp700I GAANNNNTTC 1 cut(s) 938
AspS9I GGNCC 1 cut(s) 764
AsuHPI GGTGA 1 cut(s) 364
AsuII TTCGAA 1 cut(s) 828
AvaII GGWCC 1 cut(s) 764
BanI GGYRCC 1 cut(s) 730
BauI CACGAG 1 cut(s) 159
BbrPI CACGTG 1 cut(s) 676
BbsI GAAGAC 2 cut(s) 430, 618
BbvI GCAGC 1 cut(s) 1057
BccI CCATC 1 cut(s) 87
BceAI ACGGC 3 cut(s) 45, 474, 707
BcgI CGANNNNNNTGC 4 cut(s) 450, 484, 797, 831
BciT130I CCWGG 1 cut(s) 1068
BcoDI GTCTC 4 cut(s) 435, 544, 546, 854
BfaI CTAG 1 cut(s) 747
BfrI CTTAAG 1 cut(s) 515
BisI GCNGC 1 cut(s) 1046
BlsI GCNGC 1 cut(s) 1047
Bme1390I CCNGG 1 cut(s) 1068
Bme18I GGWCC 1 cut(s) 764
BmgT120I GGNCC 1 cut(s) 764
BmiI GGNNCC 2 cut(s) 732, 912
BmrFI CCNGG 1 cut(s) 1068
BmsI GCATC 3 cut(s) 157, 169, 945
BpiI GAAGAC 2 cut(s) 430, 618
BplI GAGNNNNNCTC 2 cut(s) 111, 143
BpmI CTGGAG 1 cut(s) 374
Bpu10I CCTNAGC 1 cut(s) 56
Bpu14I TTCGAA 1 cut(s) 828
BsaAI YACGTR 2 cut(s) 665, 676
BsaHI GRCGYC 2 cut(s) 620, 720
BsaJI CCNNGG 2 cut(s) 906, 1040
BsaWI WCCGGW 1 cut(s) 433
BsaXI ACNNNNNCTCC 4 cut(s) 12, 42, 557, 587
Bse3DI GCAATG 1 cut(s) 932
BseBI CCWGG 1 cut(s) 1068
BseDI CCNNGG 2 cut(s) 906, 1040
BseGI GGATG 2 cut(s) 79, 990
BseMI GCAATG 1 cut(s) 932
BseMII CTCAG 2 cut(s) 285, 939
BseRI GAGGAG 3 cut(s) 33, 36, 217
BseXI GCAGC 1 cut(s) 1057
BsgI GTGCAG 1 cut(s) 1075
BshFI GGCC 1 cut(s) 546
BshNI GGYRCC 1 cut(s) 730
BsiSI CCGG 3 cut(s) 434, 543, 767
BsiWI CGTACG 1 cut(s) 661
BslFI GGGAC 1 cut(s) 1095
BsmAI GTCTC 4 cut(s) 435, 544, 546, 854
BsmBI CGTCTC 1 cut(s) 546
BsmFI GGGAC 1 cut(s) 1095
BsmI GAATGC 1 cut(s) 958
BsnI GGCC 1 cut(s) 546
Bsp119I TTCGAA 1 cut(s) 828
Bsp143I GATC 5 cut(s) 247, 290, 417, 452, 526
Bsp19I CCATGG 1 cut(s) 906
BspANI GGCC 1 cut(s) 546
BspCNI CTCAG 2 cut(s) 286, 940
BspLI GGNNCC 2 cut(s) 732, 912
BspPI GGATC 3 cut(s) 298, 412, 521
BspT104I TTCGAA 1 cut(s) 828
BspT107I GGYRCC 1 cut(s) 730
BspTI CTTAAG 1 cut(s) 515
BsrDI GCAATG 1 cut(s) 932
BssECI CCNNGG 2 cut(s) 906, 1040
BssMI GATC 5 cut(s) 247, 290, 417, 452, 526
BssNI GRCGYC 2 cut(s) 620, 720
BssSI CACGAG 1 cut(s) 159
BssT1I CCWWGG 1 cut(s) 906
Bst2BI CACGAG 1 cut(s) 159
Bst2UI CCWGG 1 cut(s) 1068
Bst4CI ACNGT 6 cut(s) 365, 475, 538, 557, 1033, 1053
Bst6I CTCTTC 1 cut(s) 28
BstACI GRCGYC 2 cut(s) 620, 720
BstAFI CTTAAG 1 cut(s) 515
BstBAI YACGTR 2 cut(s) 665, 676
BstBI TTCGAA 1 cut(s) 828
BstC8I GCNNGC 2 cut(s) 899, 923
BstDEI CTNAG 4 cut(s) 56, 294, 305, 948
BstDSI CCRYGG 1 cut(s) 906
BstF5I GGATG 2 cut(s) 79, 990
BstKTI GATC 5 cut(s) 250, 293, 420, 455, 529
BstMAI GTCTC 4 cut(s) 435, 544, 546, 854
BstMBI GATC 5 cut(s) 247, 290, 417, 452, 526
BstMWI GCNNNNNNNGC 1 cut(s) 657
BstNI CCWGG 1 cut(s) 1068
BstSCI CCNGG 1 cut(s) 1066
BstV1I GCAGC 1 cut(s) 1057
BstV2I GAAGAC 2 cut(s) 430, 618
BstX2I RGATCY 3 cut(s) 290, 417, 526
BstYI RGATCY 3 cut(s) 290, 417, 526
BsuRI GGCC 1 cut(s) 546
BtgI CCRYGG 1 cut(s) 906
BtgZI GCGATG 1 cut(s) 180
BtsCI GGATG 2 cut(s) 79, 990
Cac8I GCNNGC 2 cut(s) 899, 923
Cfr13I GGNCC 1 cut(s) 764
CseI GACGC 3 cut(s) 164, 383, 728
Csp6I GTAC 1 cut(s) 662
CviAII CATG 4 cut(s) 172, 837, 907, 967
CviQI GTAC 1 cut(s) 662
DdeI CTNAG 4 cut(s) 56, 294, 305, 948
DpnI GATC 5 cut(s) 249, 292, 419, 454, 528
DpnII GATC 5 cut(s) 247, 290, 417, 452, 526
EaeI YGGCCR 1 cut(s) 544
Eam1104I CTCTTC 1 cut(s) 28
EarI CTCTTC 1 cut(s) 28
Eco130I CCWWGG 1 cut(s) 906
Eco47I GGWCC 1 cut(s) 764
Eco57I CTGAAG 1 cut(s) 399
Eco72I CACGTG 1 cut(s) 676
EcoRI GAATTC 1 cut(s) 991
EcoRII CCWGG 1 cut(s) 1066
EcoT14I CCWWGG 1 cut(s) 906
EcoT22I ATGCAT 2 cut(s) 184, 960
ErhI CCWWGG 1 cut(s) 906
Esp3I CGTCTC 1 cut(s) 546
FaeI CATG 4 cut(s) 175, 840, 910, 970
FalI AAGNNNNNCTT 2 cut(s) 886, 918
FaqI GGGAC 1 cut(s) 1095
FatI CATG 4 cut(s) 171, 836, 906, 966
FblI GTMKAC 3 cut(s) 470, 598, 614
Fnu4HI GCNGC 1 cut(s) 1046
FokI GGATG 2 cut(s) 66, 997
Fsp4HI GCNGC 1 cut(s) 1046
FspBI CTAG 1 cut(s) 747
GluI GCNGC 1 cut(s) 1046
GsuI CTGGAG 1 cut(s) 374
HaeIII GGCC 1 cut(s) 546
HapII CCGG 3 cut(s) 434, 543, 767
HgaI GACGC 3 cut(s) 164, 383, 728
Hin1I GRCGYC 2 cut(s) 620, 720
Hin1II CATG 4 cut(s) 175, 840, 910, 970
HincII GTYRAC 3 cut(s) 76, 471, 599
HindII GTYRAC 3 cut(s) 76, 471, 599
HindIII AAGCTT 2 cut(s) 345, 823
HinfI GANTC 5 cut(s) 120, 298, 428, 485, 590
HpaII CCGG 3 cut(s) 434, 543, 767
HphI GGTGA 1 cut(s) 364
Hpy166II GTNNAC 5 cut(s) 76, 471, 599, 615, 878
Hpy188I TCNGA 5 cut(s) 131, 268, 295, 412, 531
Hpy188III TCNNGA 2 cut(s) 391, 640
Hpy8I GTNNAC 5 cut(s) 76, 471, 599, 615, 878
Hpy99I CGWCG 6 cut(s) 460, 472, 565, 622, 625, 722
HpyAV CCTTC 3 cut(s) 497, 824, 912
HpyCH4III ACNGT 6 cut(s) 365, 475, 538, 557, 1033, 1053
HpyCH4IV ACGT 7 cut(s) 72, 407, 467, 620, 664, 675, 1090
HpyCH4V TGCA 3 cut(s) 182, 958, 1056
HpyF10VI GCNNNNNNNGC 1 cut(s) 657
HpyF3I CTNAG 4 cut(s) 56, 294, 305, 948
HpySE526I ACGT 7 cut(s) 72, 407, 467, 620, 664, 675, 1090
Hsp92I GRCGYC 2 cut(s) 620, 720
Hsp92II CATG 4 cut(s) 175, 840, 910, 970
Kzo9I GATC 5 cut(s) 247, 290, 417, 452, 526
LmnI GCTCC 3 cut(s) 833, 895, 916
Lsp1109I GCAGC 1 cut(s) 1057
LweI GCATC 3 cut(s) 157, 169, 945
MaeI CTAG 1 cut(s) 747
MaeII ACGT 7 cut(s) 72, 407, 467, 620, 664, 675, 1090
MaeIII GTNAC 5 cut(s) 68, 352, 475, 578, 678
MalI GATC 5 cut(s) 249, 292, 419, 454, 528
MboI GATC 5 cut(s) 247, 290, 417, 452, 526
MfeI CAATTG 1 cut(s) 864
MflI RGATCY 3 cut(s) 290, 417, 526
MluCI AATT 4 cut(s) 40, 864, 971, 991
MlyI GAGTC 3 cut(s) 129, 437, 599
Mph1103I ATGCAT 2 cut(s) 184, 960
MroXI GAANNNNTTC 1 cut(s) 938
MseI TTAA 2 cut(s) 516, 1035
MslI CAYNNNNRTG 2 cut(s) 851, 1089
MspCI CTTAAG 1 cut(s) 515
MspI CCGG 3 cut(s) 434, 543, 767
MspR9I CCNGG 1 cut(s) 1068
MunI CAATTG 1 cut(s) 864
Mva1269I GAATGC 1 cut(s) 958
MvaI CCWGG 1 cut(s) 1068
MwoI GCNNNNNNNGC 1 cut(s) 657
NcoI CCATGG 1 cut(s) 906
NdeII GATC 5 cut(s) 247, 290, 417, 452, 526
NlaIII CATG 4 cut(s) 175, 840, 910, 970
NlaIV GGNNCC 2 cut(s) 732, 912
NmuCI GTSAC 3 cut(s) 68, 352, 475
NsiI ATGCAT 2 cut(s) 184, 960
NspV TTCGAA 1 cut(s) 828
PcsI WCGNNNNNNNCGW 4 cut(s) 162, 464, 617, 626
PctI GAATGC 1 cut(s) 958
PdmI GAANNNNTTC 1 cut(s) 938
PfeI GAWTC 2 cut(s) 298, 485
Pfl23II CGTACG 1 cut(s) 661
PflFI GACNNNGTC 1 cut(s) 622
PfoI TCCNGGA 1 cut(s) 1066
PkrI GCNGC 1 cut(s) 1047
PleI GAGTC 3 cut(s) 128, 436, 598
PmaCI CACGTG 1 cut(s) 676
PmlI CACGTG 1 cut(s) 676
PpsI GAGTC 3 cut(s) 128, 436, 598
Ppu21I YACGTR 2 cut(s) 665, 676
Psp6I CCWGG 1 cut(s) 1066
PspCI CACGTG 1 cut(s) 676
PspGI CCWGG 1 cut(s) 1066
PspLI CGTACG 1 cut(s) 661
PspN4I GGNNCC 2 cut(s) 732, 912
PspPI GGNCC 1 cut(s) 764
PsuI RGATCY 3 cut(s) 290, 417, 526
PsyI GACNNNGTC 1 cut(s) 622
RsaI GTAC 1 cut(s) 663
RsaNI GTAC 1 cut(s) 662
RseI CAYNNNNRTG 2 cut(s) 851, 1089
SalI GTCGAC 2 cut(s) 469, 597
SaqAI TTAA 2 cut(s) 516, 1035
SatI GCNGC 1 cut(s) 1046
Sau3AI GATC 5 cut(s) 247, 290, 417, 452, 526
Sau96I GGNCC 1 cut(s) 764
SchI GAGTC 3 cut(s) 129, 437, 599
ScrFI CCNGG 1 cut(s) 1068
SfaNI GCATC 3 cut(s) 157, 169, 945
SfuI TTCGAA 1 cut(s) 828
SinI GGWCC 1 cut(s) 764
SmiMI CAYNNNNRTG 2 cut(s) 851, 1089
SmlI CTYRAG 1 cut(s) 515
SmoI CTYRAG 1 cut(s) 515
Sse9I AATT 4 cut(s) 40, 864, 971, 991
SspMI CTAG 1 cut(s) 747
StyD4I CCNGG 1 cut(s) 1066
StyI CCWWGG 1 cut(s) 906
TaaI ACNGT 6 cut(s) 365, 475, 538, 557, 1033, 1053
TaiI ACGT 7 cut(s) 75, 410, 470, 623, 667, 678, 1093
TaqI TCGA 9 cut(s) 455, 470, 560, 598, 629, 656, 817, 828, 979
TasI AATT 4 cut(s) 40, 864, 971, 991
TfiI GAWTC 2 cut(s) 298, 485
Tru1I TTAA 2 cut(s) 516, 1035
Tru9I TTAA 2 cut(s) 516, 1035
TseFI GTSAC 3 cut(s) 68, 352, 475
TseI GCWGC 1 cut(s) 1045
Tsp45I GTSAC 3 cut(s) 68, 352, 475
TspDTI ATGAA 8 cut(s) 89, 288, 707, 795, 869, 983, 984, 1001
TspGWI ACGGA 3 cut(s) 494, 578, 697
Tth111I GACNNNGTC 1 cut(s) 622
Vha464I CTTAAG 1 cut(s) 515
VpaK11BI GGWCC 1 cut(s) 764
XapI RAATTY 2 cut(s) 40, 991
XmiI GTMKAC 3 cut(s) 470, 598, 614
XmnI GAANNNNTTC 1 cut(s) 938
XspI CTAG 1 cut(s) 747
ZraI GACGTC 1 cut(s) 621
Zsp2I ATGCAT 2 cut(s) 184, 960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.