RorugPtG0000700

Domain of unknown function (DUF966)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000008
OG0014395
Physical Location & Seq
Reverse (-)
14349 .. 16028
1680 bp
Loading structure...
UTR
Exon/CDS
Intron
RorugPtG0000700.1

Sequence Viewer

Length: 1272 bp
ATGACAAGAAGATATTGGAACATCAATTTGGAAGAGATGATGGAAGCAGGAGTTCATTTTGGTCATGGTACTAGGAAATGGAATCCTAGGATGGCACCTTATATCTCTGCAAAGCGAAAAGGTATTCATATTATAAATCTTACTAGAACTGCTCGGTTTTTATCAGAAGCTTGTGATTTAGTTTTTGATGCAGCAAGTAGGGGAAAACAATTCTTAATTGTCGGTACAAAAAATAAAGCGGCTGATTCAGTAGCGCGGGCTGCAATAAGGGCTCGGTGTCATTATGTTAATAAAAAATGGCTCGGTGGTATGTTAACGAATTGGTATACTACAGAAACGAGACTTCATAAGTTCAGGGACTTGAGAACGGAACAAAAGACGGGGAGACTTAACCGTCTTCCGAAAAGAGATGCGGCTGTGTTGAAGAGACAATTATTTCACTTGCAAACATATCTGGGCGGAATAAAATATATGACGGGGTTACCCGATATTGTAATAATCGTTGATCAGCAAGAAGAATATACGGCTCTTCGAGAATGTATCACTTTGGGAATTCCAACGATTTGTTTAATTGATACAAATTGTGACCCGGATCTCGCAGATATTTCGATTCCAGCCAACGATGACGCTATAGCTTCAATCCGATTAATTCTTAACAAATTAGTATTTGCAATTTGTGAGGGTTCAGCCATTATAGCTGTTCGTAATCCACAAACCATTCCTACTGACGGTCAGAATTTCTTTGAATATGTCCTTGAATTCATTCGAGACGTGAGCAAAACTCAAATTGGAGAAGAATATGGTCCATGGGTTCCCTTTATTGGAACTATGTTTCTATTTATTTTTGTTTCGAATTGGTCAGGGGCTCTTTTACCCTGGAAAATTATCCAGCTACCTCATGGGGAGTTAGCCGCACCCACAAATGATATAAACACGACCGTTGCTTTAGCTTTACTCACGTCAGTAGCATATTTTTATGCGGGCCTTTCAAAAAAGGGATTGGGTTATTTCGGTAAATATATTCAACCAACCCCAATCCTTTTACCTATTAACATCTTAGAAGATTTCACAAAACCGTTATCGCTTAGTTTTCGACTTTTCGGAAATATTTTAGCTGATGAATTAGTAGTTGTTGTTCTTGTTTCTTTAGTACCTTTAGTAGTTCCTATACCTGTCATGTTCCTTGGATTATTTACAAGCGGTATTCAAGCTCTTATTTTTGCAACCCTAGCTGCGGCTTATATAGGCGAATCCATGGAAGGTCATCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF966

Protein Analysis

423

Amino Acids

47.36

Weight (kDa)

8.88

Isoelectric Point (pI)

35.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S2 PF00318 13 - 228 5.6e-81 Ribosomal protein S2
ATP-synt_A PF00119 225 - 415 2e-47 ATP synthase A chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014395)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05577
fragaria_vesca FvH4_2g11530
malus_domestica MD07G1001300.v1.1 MD07G1001400.v1.1
prunus_persica Prupe.2G001400_v2.0.a1 Prupe.2G001400_v2.0.a1
pyrus_communis pycom07g00180
rosa_chinensis RchiOBHm_Chr1g0313061
rosa_laevigata RLG00000030824
rosa_multiflora Rmu_sc0001606.1_g000003
rosa_roxburghii Rroxscaffold_4G00332510
rosa_rugosa RorugPtG0000700
rosa_samantha Rh1AG000600 Rh1CG002200 Rh1CG004800 Rh1DG000300
rosa_wichuraiana Rw0G008490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 134
AasI GACNNNNNNGTC 1 cut(s) 393
AccB1I GGYRCC 1 cut(s) 94
AccI GTMKAC 1 cut(s) 326
AccII CGCG 1 cut(s) 256
AciI CCGC 8 cut(s) 239, 256, 413, 459, 912, 980, 1200, 1235
AclWI GGATC 1 cut(s) 600
AcsI RAATTY 3 cut(s) 552, 736, 758
AfaI GTAC 3 cut(s) 70, 226, 1152
AfiI CCNNNNNNNGG 3 cut(s) 728, 821, 1234
AgsI TTSAA 7 cut(s) 424, 639, 746, 758, 990, 1025, 1208
AjiI CACGTC 2 cut(s) 772, 960
AjnI CCWGG 1 cut(s) 875
AjuI GAANNNNNNNTTGG 2 cut(s) 11, 43
AluBI AGCT 8 cut(s) 170, 635, 698, 892, 950, 1115, 1211, 1232
AluI AGCT 8 cut(s) 170, 635, 698, 892, 950, 1115, 1211, 1232
Alw26I GTCTC 4 cut(s) 334, 379, 421, 762
AlwI GGATC 1 cut(s) 600
AoxI GGCC 1 cut(s) 982
ApeKI GCWGC 3 cut(s) 191, 260, 1232
ApoI RAATTY 3 cut(s) 552, 736, 758
AseI ATTAAT 1 cut(s) 647
Asp700I GAANNNNTTC 1 cut(s) 762
AspA2I CCTAGG 1 cut(s) 86
AspLEI GCGC 1 cut(s) 256
AspS9I GGNCC 2 cut(s) 803, 982
AsuC2I CCSGG 1 cut(s) 590
AsuII TTCGAA 1 cut(s) 851
AvaII GGWCC 1 cut(s) 803
AvrII CCTAGG 1 cut(s) 86
BanI GGYRCC 1 cut(s) 94
BanII GRGCYC 2 cut(s) 274, 868
BbsI GAAGAC 1 cut(s) 389
BbvI GCAGC 3 cut(s) 203, 247, 1219
BccI CCATC 2 cut(s) 34, 85
BceAI ACGGC 1 cut(s) 540
BcgI CGANNNNNNTGC 2 cut(s) 588, 622
BciT130I CCWGG 1 cut(s) 877
BclI TGATCA 1 cut(s) 505
BcnI CCSGG 1 cut(s) 590
BcoDI GTCTC 4 cut(s) 334, 379, 421, 762
BfaI CTAG 4 cut(s) 72, 87, 144, 1229
BfmI CTRYAG 2 cut(s) 330, 630
BisI GCNGC 7 cut(s) 192, 240, 261, 414, 912, 1233, 1236
BlnI CCTAGG 1 cut(s) 86
BlsI GCNGC 7 cut(s) 193, 241, 262, 415, 913, 1234, 1237
Bme1390I CCNGG 2 cut(s) 590, 877
Bme18I GGWCC 1 cut(s) 803
BmgBI CACGTC 2 cut(s) 772, 960
BmgT120I GGNCC 2 cut(s) 803, 982
BmiI GGNNCC 2 cut(s) 96, 813
BmrFI CCNGG 2 cut(s) 590, 877
BmsI GCATC 2 cut(s) 178, 400
BpiI GAAGAC 1 cut(s) 389
BplI GAGNNNNNCTC 2 cut(s) 766, 798
Bpu14I TTCGAA 1 cut(s) 851
BpuEI CTTGAG 1 cut(s) 382
BpuMI CCSGG 1 cut(s) 590
BsaJI CCNNGG 5 cut(s) 86, 806, 875, 1183, 1254
Bsc4I CCNNNNNNNGG 3 cut(s) 728, 821, 1234
BseBI CCWGG 1 cut(s) 877
BseDI CCNNGG 5 cut(s) 86, 806, 875, 1183, 1254
BseGI GGATG 1 cut(s) 96
BseLI CCNNNNNNNGG 3 cut(s) 728, 821, 1234
BseXI GCAGC 3 cut(s) 203, 247, 1219
Bsh1236I CGCG 1 cut(s) 256
Bsh1285I CGRYCG 1 cut(s) 939
BshFI GGCC 1 cut(s) 984
BshNI GGYRCC 1 cut(s) 94
BsiEI CGRYCG 1 cut(s) 939
BsiSI CCGG 1 cut(s) 590
BslFI GGGAC 1 cut(s) 371
BslI CCNNNNNNNGG 3 cut(s) 728, 821, 1234
BsmAI GTCTC 4 cut(s) 334, 379, 421, 762
BsmBI CGTCTC 1 cut(s) 762
BsmFI GGGAC 1 cut(s) 371
BsnI GGCC 1 cut(s) 984
Bsp119I TTCGAA 1 cut(s) 851
Bsp1286I GDGCHC 2 cut(s) 274, 868
Bsp143I GATC 2 cut(s) 505, 592
Bsp19I CCATGG 2 cut(s) 806, 1254
BspACI CCGC 8 cut(s) 239, 256, 413, 459, 912, 980, 1200, 1235
BspANI GGCC 1 cut(s) 984
BspFNI CGCG 1 cut(s) 256
BspLI GGNNCC 2 cut(s) 96, 813
BspPI GGATC 1 cut(s) 600
BspQI GCTCTTC 1 cut(s) 534
BspT104I TTCGAA 1 cut(s) 851
BspT107I GGYRCC 1 cut(s) 94
BssECI CCNNGG 5 cut(s) 86, 806, 875, 1183, 1254
BssMI GATC 2 cut(s) 505, 592
BssNAI GTATAC 1 cut(s) 327
BssT1I CCWWGG 4 cut(s) 86, 806, 1183, 1254
Bst1107I GTATAC 1 cut(s) 327
Bst2UI CCWGG 1 cut(s) 877
Bst4CI ACNGT 4 cut(s) 395, 731, 940, 1077
Bst6I CTCTTC 3 cut(s) 27, 419, 534
BstBI TTCGAA 1 cut(s) 851
BstC8I GCNNGC 2 cut(s) 258, 982
BstDEI CTNAG 2 cut(s) 1057, 1085
BstDSI CCRYGG 2 cut(s) 806, 1254
BstEII GGTNACC 1 cut(s) 480
BstF5I GGATG 1 cut(s) 96
BstFNI CGCG 1 cut(s) 256
BstHHI GCGC 1 cut(s) 256
BstKTI GATC 2 cut(s) 508, 595
BstMAI GTCTC 4 cut(s) 334, 379, 421, 762
BstMBI GATC 2 cut(s) 505, 592
BstMCI CGRYCG 1 cut(s) 939
BstMWI GCNNNNNNNGC 4 cut(s) 260, 269, 695, 1229
BstNI CCWGG 1 cut(s) 877
BstPI GGTNACC 1 cut(s) 480
BstSCI CCNGG 2 cut(s) 588, 875
BstSFI CTRYAG 2 cut(s) 330, 630
BstUI CGCG 1 cut(s) 256
BstV1I GCAGC 3 cut(s) 203, 247, 1219
BstV2I GAAGAC 1 cut(s) 389
BstX2I RGATCY 1 cut(s) 592
BstYI RGATCY 1 cut(s) 592
BstZ17I GTATAC 1 cut(s) 327
BsuRI GGCC 1 cut(s) 984
BtgI CCRYGG 2 cut(s) 806, 1254
BtrI CACGTC 2 cut(s) 772, 960
BtsCI GGATG 1 cut(s) 96
Cac8I GCNNGC 2 cut(s) 258, 982
CfoI GCGC 1 cut(s) 256
Cfr13I GGNCC 2 cut(s) 803, 982
CseI GACGC 1 cut(s) 635
Csp6I GTAC 3 cut(s) 69, 225, 1151
CviAII CATG 5 cut(s) 65, 807, 899, 1177, 1255
CviQI GTAC 3 cut(s) 69, 225, 1151
DdeI CTNAG 2 cut(s) 1057, 1085
DpnI GATC 2 cut(s) 507, 594
DpnII GATC 2 cut(s) 505, 592
DrdI GACNNNNNNGTC 1 cut(s) 393
DseDI GACNNNNNNGTC 1 cut(s) 393
Eam1104I CTCTTC 3 cut(s) 27, 419, 534
EarI CTCTTC 3 cut(s) 27, 419, 534
EciI GGCGGA 1 cut(s) 474
Eco130I CCWWGG 4 cut(s) 86, 806, 1183, 1254
Eco24I GRGCYC 2 cut(s) 274, 868
Eco47I GGWCC 1 cut(s) 803
Eco91I GGTNACC 1 cut(s) 480
EcoO65I GGTNACC 1 cut(s) 480
EcoRI GAATTC 2 cut(s) 552, 758
EcoRII CCWGG 1 cut(s) 875
EcoT14I CCWWGG 4 cut(s) 86, 806, 1183, 1254
EcoT38I GRGCYC 2 cut(s) 274, 868
ErhI CCWWGG 4 cut(s) 86, 806, 1183, 1254
Esp3I CGTCTC 1 cut(s) 762
FaeI CATG 5 cut(s) 68, 810, 902, 1180, 1258
FaqI GGGAC 1 cut(s) 371
FatI CATG 5 cut(s) 64, 806, 898, 1176, 1254
FauI CCCGC 2 cut(s) 249, 973
FbaI TGATCA 1 cut(s) 505
FblI GTMKAC 1 cut(s) 326
Fnu4HI GCNGC 7 cut(s) 192, 240, 261, 414, 912, 1233, 1236
FokI GGATG 1 cut(s) 103
FriOI GRGCYC 2 cut(s) 274, 868
Fsp4HI GCNGC 7 cut(s) 192, 240, 261, 414, 912, 1233, 1236
FspBI CTAG 4 cut(s) 72, 87, 144, 1229
GlaI GCGC 1 cut(s) 255
GluI GCNGC 7 cut(s) 192, 240, 261, 414, 912, 1233, 1236
HaeIII GGCC 1 cut(s) 984
HapII CCGG 1 cut(s) 590
HgaI GACGC 1 cut(s) 635
HhaI GCGC 1 cut(s) 256
Hin1II CATG 5 cut(s) 68, 810, 902, 1180, 1258
Hin6I GCGC 1 cut(s) 254
HinP1I GCGC 1 cut(s) 254
HincII GTYRAC 1 cut(s) 315
HindII GTYRAC 1 cut(s) 315
HindIII AAGCTT 1 cut(s) 168
HinfI GANTC 4 cut(s) 82, 245, 610, 1250
HpaI GTTAAC 1 cut(s) 315
HpaII CCGG 1 cut(s) 590
Hpy166II GTNNAC 2 cut(s) 315, 327
Hpy188I TCNGA 5 cut(s) 166, 402, 644, 735, 1103
Hpy188III TCNNGA 2 cut(s) 533, 767
Hpy8I GTNNAC 2 cut(s) 315, 327
HpyAV CCTTC 1 cut(s) 1253
HpyCH4III ACNGT 4 cut(s) 395, 731, 940, 1077
HpyCH4IV ACGT 2 cut(s) 771, 959
HpyCH4V TGCA 6 cut(s) 110, 191, 263, 445, 671, 1223
HpyF10VI GCNNNNNNNGC 4 cut(s) 260, 269, 695, 1229
HpyF3I CTNAG 2 cut(s) 1057, 1085
HpySE526I ACGT 2 cut(s) 771, 959
Hsp92II CATG 5 cut(s) 68, 810, 902, 1180, 1258
HspAI GCGC 1 cut(s) 254
Ksp22I TGATCA 1 cut(s) 505
KspAI GTTAAC 1 cut(s) 315
Kzo9I GATC 2 cut(s) 505, 592
LguI GCTCTTC 1 cut(s) 534
Lsp1109I GCAGC 3 cut(s) 203, 247, 1219
LweI GCATC 2 cut(s) 178, 400
MaeI CTAG 4 cut(s) 72, 87, 144, 1229
MaeII ACGT 2 cut(s) 771, 959
MaeIII GTNAC 2 cut(s) 480, 584
MalI GATC 2 cut(s) 507, 594
MboI GATC 2 cut(s) 505, 592
MboII GAAGA 8 cut(s) 21, 44, 389, 436, 521, 527, 806, 1073
MflI RGATCY 1 cut(s) 592
MhlI GDGCHC 2 cut(s) 274, 868
MmeI TCCRAC 1 cut(s) 581
MnlI CCTC 2 cut(s) 673, 906
MroXI GAANNNNTTC 1 cut(s) 762
MseI TTAA 8 cut(s) 215, 288, 314, 390, 569, 647, 654, 1050
MspI CCGG 1 cut(s) 590
MspR9I CCNGG 2 cut(s) 590, 877
MvaI CCWGG 1 cut(s) 877
MvnI CGCG 1 cut(s) 256
MwoI GCNNNNNNNGC 4 cut(s) 260, 269, 695, 1229
NciI CCSGG 1 cut(s) 590
NcoI CCATGG 2 cut(s) 806, 1254
NdeII GATC 2 cut(s) 505, 592
NlaIII CATG 5 cut(s) 68, 810, 902, 1180, 1258
NlaIV GGNNCC 2 cut(s) 96, 813
NmuCI GTSAC 1 cut(s) 584
NspV TTCGAA 1 cut(s) 851
PciSI GCTCTTC 1 cut(s) 534
PdmI GAANNNNTTC 1 cut(s) 762
PfeI GAWTC 4 cut(s) 82, 245, 610, 1250
PkrI GCNGC 7 cut(s) 193, 241, 262, 415, 913, 1234, 1237
PshBI ATTAAT 1 cut(s) 647
PsiI TTATAA 1 cut(s) 134
Psp6I CCWGG 1 cut(s) 875
PspEI GGTNACC 1 cut(s) 480
PspGI CCWGG 1 cut(s) 875
PspN4I GGNNCC 2 cut(s) 96, 813
PspPI GGNCC 2 cut(s) 803, 982
PsrI GAACNNNNNNTAC 2 cut(s) 1119, 1151
PsuI RGATCY 1 cut(s) 592
RsaI GTAC 3 cut(s) 70, 226, 1152
RsaNI GTAC 3 cut(s) 69, 225, 1151
SapI GCTCTTC 1 cut(s) 534
SaqAI TTAA 8 cut(s) 215, 288, 314, 390, 569, 647, 654, 1050
SatI GCNGC 7 cut(s) 192, 240, 261, 414, 912, 1233, 1236
Sau3AI GATC 2 cut(s) 505, 592
Sau96I GGNCC 2 cut(s) 803, 982
ScrFI CCNGG 2 cut(s) 590, 877
SduI GDGCHC 2 cut(s) 274, 868
SfaNI GCATC 2 cut(s) 178, 400
SfcI CTRYAG 2 cut(s) 330, 630
SfuI TTCGAA 1 cut(s) 851
SinI GGWCC 1 cut(s) 803
SmlI CTYRAG 1 cut(s) 361
SmoI CTYRAG 1 cut(s) 361
SsiI CCGC 8 cut(s) 239, 256, 413, 459, 912, 980, 1200, 1235
SspI AATATT 1 cut(s) 1108
SspMI CTAG 4 cut(s) 72, 87, 144, 1229
StyD4I CCNGG 2 cut(s) 588, 875
StyI CCWWGG 4 cut(s) 86, 806, 1183, 1254
TaaI ACNGT 4 cut(s) 395, 731, 940, 1077
TaiI ACGT 2 cut(s) 774, 962
TaqI TCGA 5 cut(s) 532, 608, 766, 851, 1093
TauI GCSGC 4 cut(s) 242, 416, 914, 1238
TfiI GAWTC 4 cut(s) 82, 245, 610, 1250
Tru1I TTAA 8 cut(s) 215, 288, 314, 390, 569, 647, 654, 1050
Tru9I TTAA 8 cut(s) 215, 288, 314, 390, 569, 647, 654, 1050
TseFI GTSAC 1 cut(s) 584
TseI GCWGC 3 cut(s) 191, 260, 1232
Tsp45I GTSAC 1 cut(s) 584
TspDTI ATGAA 5 cut(s) 44, 116, 335, 751, 1134
TspGWI ACGGA 1 cut(s) 383
VpaK11BI GGWCC 1 cut(s) 803
VspI ATTAAT 1 cut(s) 647
XapI RAATTY 3 cut(s) 552, 736, 758
XcmI CCANNNNNNNNNTGG 1 cut(s) 896
XmaJI CCTAGG 1 cut(s) 86
XmiI GTMKAC 1 cut(s) 326
XmnI GAANNNNTTC 1 cut(s) 762
XspI CTAG 4 cut(s) 72, 87, 144, 1229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.