Rh1DG000300

Domain of unknown function (DUF966)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0014395
Physical Location & Seq
Reverse (-)
127699 .. 135502
7804 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG000300.1

Sequence Viewer

Length: 1215 bp
ATGGAAGGCAGAGGCGGAGTAGTAGGTGAAATACGGCGACTTAACATCATCTACTTCCTATGTAGTCTTGGCCGCATTGATCATCCCCATCTCATTCGTGTCCATCATCTTAACCGAAACGGCGTTTTTCTGCGAGACATCAAGAGGTGGCTTGCGGATTTACGAGGGAAGGATATGCCAGAGACCTTCACTTGGTCTTACAAGAGGAGGTACAAGAAGGGTTATGTTTGGCAAGACTTGTTAGATGACGACCTCATCACCCCTATCTCTGACAATGAATATGTCCTCAAAGGCTCCCAAATTCTCTCTTATTCCTCTTTTGCTCCTCCGCATTCATATTTATATGCTGCTGAAAAGAAGAGGTGCTTGCTCAAAGAGCTGCAGCCACCTAATTCTGAAGAAGCGCCCTTTCAAATGCACCATATTACAAAAACTTCCTTTGAAACGGACCAGCAAGAATCACCACCTCATAATTCCGGCTCATCTTTGATCGATGCTGATGCAGTGAACATCCGAGGAGATCAGAAATGCTCGTCGTCGTCCTCAACCATCAAAGCCAACAGTACAAATCACGAACAGAGCAAGGACAAGTCATCAATAGAAAACTCTTGTTTCTATTCCCATTTGTTATTGAAGAAGAAGAAGAAGTCGTCCAGCGACAAAGACAAAGACAGCAAAGAGATGGGTTGTAGTAGTACTACTTCATCATCTTCTTCTTCTCCACATAATTCATCACCTCTATCTCAATCGGTGCCCTTGGAAAAGAGTACTAAAAAACAACATTTAAGCATGTTCCGCAATTTGATCAACTGTGGTGCTGTGGACACGAACGACACCGTATTCGTCAGGTTGAATCGGGACAGTAAGACATATTCCACTAACAATAAGCCTCTTAATCGCAGCAGGCAAGACATTATTATTCGAAAAGAAAAGAGGGAGAAAGCAGGTACTGTAGGGTATGCCGGACTTTTTAGGACATGTATGAATCAGCAGGAGCGGCCACAGCAACCCCAAAACCATACTACTACTGCTAGCAAAAGCGATGATAGGAACCAAAAAACAGCTTCTTCTACTTACAGGCCAATCCGAGGGCCACATTGCTCGCATTGCAGGAAGCAGTTTAAGCCAGAGAAATTGCACTCGCACATGAAATCCTGCAAAGGAATAAAAGCTCTGTCAAAAACTGCTCCGGATTCTGTGGTCAAGATGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF966

Protein Analysis

404

Amino Acids

45.84

Weight (kDa)

9.6

Isoelectric Point (pI)

58.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOK PF06136 15 - 102 1.3e-29 SOSEKI protein DIX-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014395)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05577
fragaria_vesca FvH4_2g11530
malus_domestica MD07G1001300.v1.1 MD07G1001400.v1.1
prunus_persica Prupe.2G001400_v2.0.a1 Prupe.2G001400_v2.0.a1
pyrus_communis pycom07g00180
rosa_chinensis RchiOBHm_Chr1g0313061
rosa_laevigata RLG00000030824
rosa_multiflora Rmu_sc0001606.1_g000003
rosa_roxburghii Rroxscaffold_4G00332510
rosa_rugosa RorugPtG0000700
rosa_samantha Rh1AG000600 Rh1CG002200 Rh1CG004800 Rh1DG000300
rosa_wichuraiana Rw0G008490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 935
AccB1I GGYRCC 1 cut(s) 751
AccBSI CCGCTC 1 cut(s) 997
AccIII TCCGGA 1 cut(s) 1189
AciI CCGC 6 cut(s) 15, 73, 155, 329, 796, 997
AcoI YGGCCR 2 cut(s) 70, 998
AcsI RAATTY 1 cut(s) 300
AcuI CTGAAG 1 cut(s) 417
AfaI GTAC 5 cut(s) 212, 565, 697, 769, 949
AfiI CCNNNNNNNGG 2 cut(s) 192, 1088
AflIII ACRYGT 1 cut(s) 977
AgsI TTSAA 4 cut(s) 413, 443, 634, 853
AluBI AGCT 3 cut(s) 379, 1064, 1172
AluI AGCT 3 cut(s) 379, 1064, 1172
Alw26I GTCTC 2 cut(s) 129, 176
AlwNI CAGNNNCTG 1 cut(s) 950
Aor13HI TCCGGA 1 cut(s) 1189
AoxI GGCC 4 cut(s) 70, 998, 1079, 1091
ApeKI GCWGC 4 cut(s) 347, 379, 382, 900
ApoI RAATTY 1 cut(s) 300
ArsI GACNNNNNNTTYG 2 cut(s) 824, 856
AspLEI GCGC 1 cut(s) 406
AspS9I GGNCC 2 cut(s) 448, 1091
AsuHPI GGTGA 4 cut(s) 38, 250, 453, 726
AsuII TTCGAA 1 cut(s) 922
AsuNHI GCTAGC 1 cut(s) 1031
AvaII GGWCC 1 cut(s) 448
BaeGI GKGCMC 1 cut(s) 756
BanI GGYRCC 1 cut(s) 751
BarI GAAGNNNNNNTAC 2 cut(s) 685, 717
BbvI GCAGC 4 cut(s) 334, 366, 394, 912
BccI CCATC 4 cut(s) 96, 111, 557, 676
BceAI ACGGC 2 cut(s) 50, 136
BcgI CGANNNNNNTGC 2 cut(s) 482, 516
BclI TGATCA 2 cut(s) 79, 804
BcoDI GTCTC 2 cut(s) 129, 176
BfaI CTAG 1 cut(s) 1032
BfmI CTRYAG 2 cut(s) 380, 951
BfoI RGCGCY 1 cut(s) 407
BfuAI ACCTGC 1 cut(s) 935
BisI GCNGC 6 cut(s) 73, 348, 380, 383, 901, 998
BlsI GCNGC 6 cut(s) 74, 349, 381, 384, 902, 999
BmcAI AGTACT 2 cut(s) 697, 769
Bme18I GGWCC 1 cut(s) 448
BmgT120I GGNCC 2 cut(s) 448, 1091
BmiI GGNNCC 3 cut(s) 295, 753, 1052
BmsI GCATC 3 cut(s) 484, 490, 1197
BmtI GCTAGC 1 cut(s) 1035
Bpu14I TTCGAA 1 cut(s) 922
Bsa29I ATCGAT 1 cut(s) 492
BsaI GGTCTC 1 cut(s) 176
BsaJI CCNNGG 3 cut(s) 514, 756, 1087
BsaWI WCCGGW 1 cut(s) 1189
Bsc4I CCNNNNNNNGG 2 cut(s) 192, 1088
Bse3DI GCAATG 2 cut(s) 1096, 1105
BseAI TCCGGA 1 cut(s) 1189
BseCI ATCGAT 1 cut(s) 492
BseDI CCNNGG 3 cut(s) 514, 756, 1087
BseGI GGATG 2 cut(s) 82, 510
BseLI CCNNNNNNNGG 2 cut(s) 192, 1088
BseMI GCAATG 2 cut(s) 1096, 1105
BseRI GAGGAG 3 cut(s) 220, 315, 531
BseSI GKGCMC 1 cut(s) 756
BseXI GCAGC 4 cut(s) 334, 366, 394, 912
BshFI GGCC 4 cut(s) 72, 1000, 1081, 1093
BshNI GGYRCC 1 cut(s) 751
BshVI ATCGAT 1 cut(s) 492
BsiSI CCGG 3 cut(s) 477, 963, 1190
BslFI GGGAC 1 cut(s) 872
BslI CCNNNNNNNGG 2 cut(s) 192, 1088
BsmAI GTCTC 2 cut(s) 129, 176
BsmFI GGGAC 1 cut(s) 872
BsmI GAATGC 1 cut(s) 331
BsnI GGCC 4 cut(s) 72, 1000, 1081, 1093
Bso31I GGTCTC 1 cut(s) 176
Bsp119I TTCGAA 1 cut(s) 922
Bsp1286I GDGCHC 1 cut(s) 756
Bsp13I TCCGGA 1 cut(s) 1189
Bsp143I GATC 4 cut(s) 79, 489, 520, 804
BspACI CCGC 6 cut(s) 15, 73, 155, 329, 796, 997
BspANI GGCC 4 cut(s) 72, 1000, 1081, 1093
BspDI ATCGAT 1 cut(s) 492
BspEI TCCGGA 1 cut(s) 1189
BspLI GGNNCC 3 cut(s) 295, 753, 1052
BspMAI CTGCAG 1 cut(s) 384
BspMI ACCTGC 1 cut(s) 935
BspOI GCTAGC 1 cut(s) 1035
BspT104I TTCGAA 1 cut(s) 922
BspT107I GGYRCC 1 cut(s) 751
BspTNI GGTCTC 1 cut(s) 176
BsrBI CCGCTC 1 cut(s) 997
BsrDI GCAATG 2 cut(s) 1096, 1105
BssECI CCNNGG 3 cut(s) 514, 756, 1087
BssMI GATC 4 cut(s) 79, 489, 520, 804
BssT1I CCWWGG 1 cut(s) 756
Bst4CI ACNGT 5 cut(s) 563, 812, 838, 863, 952
Bst6I CTCTTC 1 cut(s) 353
BstBI TTCGAA 1 cut(s) 922
BstC8I GCNNGC 5 cut(s) 153, 368, 905, 1033, 1103
BstF5I GGATG 2 cut(s) 82, 510
BstH2I RGCGCY 1 cut(s) 407
BstHHI GCGC 1 cut(s) 406
BstKTI GATC 4 cut(s) 82, 492, 523, 807
BstMAI GTCTC 2 cut(s) 129, 176
BstMBI GATC 4 cut(s) 79, 489, 520, 804
BstMWI GCNNNNNNNGC 6 cut(s) 376, 795, 997, 1003, 1107, 1123
BstNSI RCATGY 2 cut(s) 793, 981
BstSFI CTRYAG 2 cut(s) 380, 951
BstSLI GKGCMC 1 cut(s) 756
BstV1I GCAGC 4 cut(s) 334, 366, 394, 912
Bsu15I ATCGAT 1 cut(s) 492
BsuRI GGCC 4 cut(s) 72, 1000, 1081, 1093
BsuTUI ATCGAT 1 cut(s) 492
BtgZI GCGATG 1 cut(s) 1056
BtsCI GGATG 2 cut(s) 82, 510
BtsI GCAGTG 1 cut(s) 510
BtsIMutI CAGTG 1 cut(s) 510
BveI ACCTGC 1 cut(s) 935
Cac8I GCNNGC 5 cut(s) 153, 368, 905, 1033, 1103
CaiI CAGNNNCTG 1 cut(s) 950
CfoI GCGC 1 cut(s) 406
Cfr13I GGNCC 2 cut(s) 448, 1091
ClaI ATCGAT 1 cut(s) 492
Csp6I GTAC 5 cut(s) 211, 564, 696, 768, 948
CviAII CATG 4 cut(s) 790, 978, 1147, 1212
CviQI GTAC 5 cut(s) 211, 564, 696, 768, 948
DpnI GATC 4 cut(s) 81, 491, 522, 806
DpnII GATC 4 cut(s) 79, 489, 520, 804
EaeI YGGCCR 2 cut(s) 70, 998
Eam1104I CTCTTC 1 cut(s) 353
EarI CTCTTC 1 cut(s) 353
EciI GGCGGA 1 cut(s) 30
Eco130I CCWWGG 1 cut(s) 756
Eco31I GGTCTC 1 cut(s) 176
Eco47I GGWCC 1 cut(s) 448
Eco57I CTGAAG 1 cut(s) 417
EcoT14I CCWWGG 1 cut(s) 756
ErhI CCWWGG 1 cut(s) 756
FaeI CATG 4 cut(s) 793, 981, 1150, 1215
FalI AAGNNNNNCTT 2 cut(s) 350, 382
FaqI GGGAC 1 cut(s) 872
FatI CATG 4 cut(s) 789, 977, 1146, 1211
FbaI TGATCA 2 cut(s) 79, 804
Fnu4HI GCNGC 6 cut(s) 73, 348, 380, 383, 901, 998
FokI GGATG 2 cut(s) 69, 497
Fsp4HI GCNGC 6 cut(s) 73, 348, 380, 383, 901, 998
FspBI CTAG 1 cut(s) 1032
GlaI GCGC 1 cut(s) 405
GluI GCNGC 6 cut(s) 73, 348, 380, 383, 901, 998
HaeII RGCGCY 1 cut(s) 407
HaeIII GGCC 4 cut(s) 72, 1000, 1081, 1093
HapII CCGG 3 cut(s) 477, 963, 1190
HhaI GCGC 1 cut(s) 406
Hin1II CATG 4 cut(s) 793, 981, 1150, 1215
Hin6I GCGC 1 cut(s) 404
HinP1I GCGC 1 cut(s) 404
HinfI GANTC 4 cut(s) 458, 853, 985, 1193
HpaII CCGG 3 cut(s) 477, 963, 1190
HphI GGTGA 4 cut(s) 38, 250, 453, 726
Hpy166II GTNNAC 2 cut(s) 508, 823
Hpy188I TCNGA 5 cut(s) 271, 397, 515, 525, 1088
Hpy188III TCNNGA 5 cut(s) 142, 572, 857, 1190, 1204
Hpy8I GTNNAC 2 cut(s) 508, 823
Hpy99I CGWCG 2 cut(s) 538, 541
HpyAV CCTTC 3 cut(s) 163, 196, 211
HpyCH4III ACNGT 5 cut(s) 563, 812, 838, 863, 952
HpyCH4V TGCA 6 cut(s) 382, 418, 503, 1110, 1138, 1158
HpyF10VI GCNNNNNNNGC 6 cut(s) 376, 795, 997, 1003, 1107, 1123
Hsp92II CATG 4 cut(s) 793, 981, 1150, 1215
HspAI GCGC 1 cut(s) 404
Kpn2I TCCGGA 1 cut(s) 1189
Ksp22I TGATCA 2 cut(s) 79, 804
Kzo9I GATC 4 cut(s) 79, 489, 520, 804
LmnI GCTCC 4 cut(s) 299, 328, 994, 1192
Lsp1109I GCAGC 4 cut(s) 334, 366, 394, 912
LweI GCATC 3 cut(s) 484, 490, 1197
MaeI CTAG 1 cut(s) 1032
MalI GATC 4 cut(s) 81, 491, 522, 806
MbiI CCGCTC 1 cut(s) 997
MboI GATC 4 cut(s) 79, 489, 520, 804
MhlI GDGCHC 1 cut(s) 756
MluCI AATT 6 cut(s) 300, 391, 472, 727, 799, 1133
MroI TCCGGA 1 cut(s) 1189
MseI TTAA 5 cut(s) 42, 111, 785, 894, 1122
MspI CCGG 3 cut(s) 477, 963, 1190
Mva1269I GAATGC 1 cut(s) 331
MwoI GCNNNNNNNGC 6 cut(s) 376, 795, 997, 1003, 1107, 1123
NdeII GATC 4 cut(s) 79, 489, 520, 804
NheI GCTAGC 1 cut(s) 1031
NlaIII CATG 4 cut(s) 793, 981, 1150, 1215
NlaIV GGNNCC 3 cut(s) 295, 753, 1052
NspI RCATGY 2 cut(s) 793, 981
NspV TTCGAA 1 cut(s) 922
PciI ACATGT 1 cut(s) 977
PctI GAATGC 1 cut(s) 331
PfeI GAWTC 4 cut(s) 458, 853, 985, 1193
PkrI GCNGC 6 cut(s) 74, 349, 381, 384, 902, 999
PscI ACATGT 1 cut(s) 977
PspN4I GGNNCC 3 cut(s) 295, 753, 1052
PspPI GGNCC 2 cut(s) 448, 1091
PstI CTGCAG 1 cut(s) 384
PstNI CAGNNNCTG 1 cut(s) 950
RsaI GTAC 5 cut(s) 212, 565, 697, 769, 949
RsaNI GTAC 5 cut(s) 211, 564, 696, 768, 948
SaqAI TTAA 5 cut(s) 42, 111, 785, 894, 1122
SatI GCNGC 6 cut(s) 73, 348, 380, 383, 901, 998
Sau3AI GATC 4 cut(s) 79, 489, 520, 804
Sau96I GGNCC 2 cut(s) 448, 1091
ScaI AGTACT 2 cut(s) 697, 769
SduI GDGCHC 1 cut(s) 756
SfaNI GCATC 3 cut(s) 484, 490, 1197
SfcI CTRYAG 2 cut(s) 380, 951
SfuI TTCGAA 1 cut(s) 922
SinI GGWCC 1 cut(s) 448
Sse9I AATT 6 cut(s) 300, 391, 472, 727, 799, 1133
SsiI CCGC 6 cut(s) 15, 73, 155, 329, 796, 997
SspMI CTAG 1 cut(s) 1032
StyI CCWWGG 1 cut(s) 756
TaaI ACNGT 5 cut(s) 563, 812, 838, 863, 952
TaqI TCGA 2 cut(s) 492, 922
TasI AATT 6 cut(s) 300, 391, 472, 727, 799, 1133
TatI WGTACW 3 cut(s) 563, 695, 767
TauI GCSGC 2 cut(s) 75, 1000
TfiI GAWTC 4 cut(s) 458, 853, 985, 1193
Tru1I TTAA 5 cut(s) 42, 111, 785, 894, 1122
Tru9I TTAA 5 cut(s) 42, 111, 785, 894, 1122
TscAI CASTG 1 cut(s) 510
TseI GCWGC 4 cut(s) 347, 379, 382, 900
TspDTI ATGAA 6 cut(s) 291, 324, 693, 720, 998, 1163
TspGWI ACGGA 1 cut(s) 461
TspRI CASTG 1 cut(s) 510
VpaK11BI GGWCC 1 cut(s) 448
XapI RAATTY 1 cut(s) 300
XceI RCATGY 2 cut(s) 793, 981
XspI CTAG 1 cut(s) 1032
ZrmI AGTACT 2 cut(s) 697, 769
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.