RLG00000030824

Domain of unknown function (DUF966)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0014395
Physical Location & Seq
Reverse (-)
68226184 .. 68229933
3750 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030824

Sequence Viewer

Length: 1218 bp
ATGGAAGGCAGAGGCGGAGTAGTAGGTGAAATACGGCGACTTAACATCATCTACTTCCTATGTAGTCTCGGCCGCATTGATCATCCCCATCTCATTCGTGTCCATCATCTTAACCGAAACGGCGTCTTTCTGCGAGACATCAAGAGGTGGCTTGCGGATTTACGAGGGAAGGATATGCCAGAGACCTTCACTTGGTCTTACAAGAGGAGGTACAAGAAGGGTTATGTTTGGCAAGACTTGTTAGATGACGACCTCATCACCCCTATCTCTGACAATGAATATGTCCTCAAAGGCTCCCAAATTCTCTCTTATTCCTCTTTTGATCCTCCGCATTCATATTTATATTCTGCTGAAAAGAAGAGGTGCTTGCTCAAAGAGCTGCAGCCACCTAATTCTGAAGAAGCGCGCTTTCAAATGCACCATATTACAAAAACTTCATTTGAAACGGACCAGCAAGAATCACCACCTCATAATTCCGGCTCATCTTTGATCGATGCTGATGCAGTGAACATCCGAGGAGATCAGAAACGCTCGTCGTCGTCCTCAACCATCAAAGCCAACAGTACAAATCACGAACAGAGCAAGGACACGTCATCAATAGAAAACTCTTGTTTCTATTCGAATTTGTTATTGAAGAAGAAGAAGAAGTGGTCCAGCGACAAAGACAGAGACAGCAAAGAGATGGGTTGTAGTAGTACTACTTCATCTTCTTCTTCTTCTCCACATAATTCATCACCTCAATCTCAATCGGTGCCCTTGGGAAAGAGTACTAAAAAACAACATTTAAGCATCTTCCGCAATTTGATCAACTGTGGTGCTGTGGACACGAACGACACCGTATTCGTCAGGTTGAATCGGGACAGTAAGACATATTCCACTAACAACAATAAGCCTCTTAATCGCAGCAGGCAAGACATTATTATTCGAAAAGAAAAGAGGGAGAAAGCAGGTACTGTAGGGTATGCCGGACTTTTTAGGACATGTATGAATCAGCAGGAGCGGCCACAGCAACCCCAAAACCATACTACTACTGCTAGCAAAAGCGATGATAGGAACCAAAAAACAGCTTCTTCTACTTACAGGCCAATCCGTGGGCCACATTGCTCGCATTGCAGGAAGCAGTTTAAGCCAGAGAAATTGCACTCGCACATGAAATCCTGCAGCGGAATAAAGGCTCTGTCAAAAACTGCTCCGGATTCTGTGGTCAAGTTGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF966

Protein Analysis

406

Amino Acids

46.06

Weight (kDa)

9.66

Isoelectric Point (pI)

57.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOK PF06136 15 - 102 1.3e-29 SOSEKI protein DIX-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014395)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05577
fragaria_vesca FvH4_2g11530
malus_domestica MD07G1001300.v1.1 MD07G1001400.v1.1
prunus_persica Prupe.2G001400_v2.0.a1 Prupe.2G001400_v2.0.a1
pyrus_communis pycom07g00180
rosa_chinensis RchiOBHm_Chr1g0313061
rosa_laevigata RLG00000030824
rosa_multiflora Rmu_sc0001606.1_g000003
rosa_roxburghii Rroxscaffold_4G00332510
rosa_rugosa RorugPtG0000700
rosa_samantha Rh1AG000600 Rh1CG002200 Rh1CG004800 Rh1DG000300
rosa_wichuraiana Rw0G008490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 938
AccB1I GGYRCC 1 cut(s) 751
AccB7I CCANNNNNTGG 1 cut(s) 1091
AccBSI CCGCTC 1 cut(s) 1000
AccII CGCG 1 cut(s) 406
AccIII TCCGGA 1 cut(s) 1192
AciI CCGC 7 cut(s) 15, 73, 155, 329, 796, 1000, 1164
AclWI GGATC 1 cut(s) 317
AcoI YGGCCR 2 cut(s) 70, 1001
AcsI RAATTY 2 cut(s) 300, 622
AcuI CTGAAG 1 cut(s) 417
AcyI GRCGYC 1 cut(s) 123
AfaI GTAC 5 cut(s) 212, 565, 697, 769, 952
AfiI CCNNNNNNNGG 2 cut(s) 192, 1091
AflIII ACRYGT 2 cut(s) 588, 980
AgsI TTSAA 4 cut(s) 413, 443, 634, 853
AjiI CACGTC 1 cut(s) 591
AluBI AGCT 2 cut(s) 379, 1067
AluI AGCT 2 cut(s) 379, 1067
Alw26I GTCTC 4 cut(s) 71, 129, 176, 663
AlwI GGATC 1 cut(s) 317
AlwNI CAGNNNCTG 1 cut(s) 953
Aor13HI TCCGGA 1 cut(s) 1192
AoxI GGCC 4 cut(s) 70, 1001, 1082, 1094
ApeKI GCWGC 4 cut(s) 379, 382, 903, 1161
ApoI RAATTY 2 cut(s) 300, 622
ArsI GACNNNNNNTTYG 2 cut(s) 824, 856
AspLEI GCGC 2 cut(s) 406, 408
AspS9I GGNCC 3 cut(s) 448, 651, 1094
AsuHPI GGTGA 4 cut(s) 38, 250, 453, 726
AsuII TTCGAA 2 cut(s) 620, 925
AsuNHI GCTAGC 1 cut(s) 1034
AvaII GGWCC 2 cut(s) 448, 651
BaeGI GKGCMC 1 cut(s) 756
BanI GGYRCC 1 cut(s) 751
BarI GAAGNNNNNNTAC 2 cut(s) 685, 717
BbvI GCAGC 4 cut(s) 366, 394, 915, 1173
BccI CCATC 4 cut(s) 96, 111, 557, 676
BceAI ACGGC 2 cut(s) 50, 136
BcgI CGANNNNNNTGC 2 cut(s) 482, 516
BclI TGATCA 2 cut(s) 79, 804
BcoDI GTCTC 4 cut(s) 71, 129, 176, 663
BfaI CTAG 1 cut(s) 1035
BfmI CTRYAG 3 cut(s) 380, 954, 1159
BfuAI ACCTGC 1 cut(s) 938
BisI GCNGC 6 cut(s) 73, 380, 383, 904, 1001, 1162
BlsI GCNGC 6 cut(s) 74, 381, 384, 905, 1002, 1163
BmcAI AGTACT 2 cut(s) 697, 769
Bme18I GGWCC 2 cut(s) 448, 651
BmgBI CACGTC 1 cut(s) 591
BmgT120I GGNCC 3 cut(s) 448, 651, 1094
BmiI GGNNCC 3 cut(s) 295, 753, 1055
BmsI GCATC 3 cut(s) 484, 490, 798
BmtI GCTAGC 1 cut(s) 1038
Bpu14I TTCGAA 2 cut(s) 620, 925
Bsa29I ATCGAT 1 cut(s) 492
BsaHI GRCGYC 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 176
BsaJI CCNNGG 3 cut(s) 514, 756, 1090
BsaWI WCCGGW 1 cut(s) 1192
Bsc4I CCNNNNNNNGG 2 cut(s) 192, 1091
Bse3DI GCAATG 2 cut(s) 1099, 1108
BseAI TCCGGA 1 cut(s) 1192
BseCI ATCGAT 1 cut(s) 492
BseDI CCNNGG 3 cut(s) 514, 756, 1090
BseGI GGATG 2 cut(s) 82, 510
BseLI CCNNNNNNNGG 2 cut(s) 192, 1091
BseMI GCAATG 2 cut(s) 1099, 1108
BsePI GCGCGC 1 cut(s) 404
BseRI GAGGAG 2 cut(s) 220, 531
BseSI GKGCMC 1 cut(s) 756
BseX3I CGGCCG 1 cut(s) 70
BseXI GCAGC 4 cut(s) 366, 394, 915, 1173
Bsh1236I CGCG 1 cut(s) 406
Bsh1285I CGRYCG 1 cut(s) 73
BshFI GGCC 4 cut(s) 72, 1003, 1084, 1096
BshNI GGYRCC 1 cut(s) 751
BshVI ATCGAT 1 cut(s) 492
BsiEI CGRYCG 1 cut(s) 73
BsiSI CCGG 3 cut(s) 477, 966, 1193
BslFI GGGAC 1 cut(s) 872
BslI CCNNNNNNNGG 2 cut(s) 192, 1091
BsmAI GTCTC 4 cut(s) 71, 129, 176, 663
BsmFI GGGAC 1 cut(s) 872
BsmI GAATGC 1 cut(s) 331
BsnI GGCC 4 cut(s) 72, 1003, 1084, 1096
Bso31I GGTCTC 1 cut(s) 176
Bsp119I TTCGAA 2 cut(s) 620, 925
Bsp1286I GDGCHC 1 cut(s) 756
Bsp13I TCCGGA 1 cut(s) 1192
Bsp143I GATC 5 cut(s) 79, 322, 489, 520, 804
BspACI CCGC 7 cut(s) 15, 73, 155, 329, 796, 1000, 1164
BspANI GGCC 4 cut(s) 72, 1003, 1084, 1096
BspDI ATCGAT 1 cut(s) 492
BspEI TCCGGA 1 cut(s) 1192
BspFNI CGCG 1 cut(s) 406
BspLI GGNNCC 3 cut(s) 295, 753, 1055
BspMAI CTGCAG 2 cut(s) 384, 1163
BspMI ACCTGC 1 cut(s) 938
BspOI GCTAGC 1 cut(s) 1038
BspPI GGATC 1 cut(s) 317
BspT104I TTCGAA 2 cut(s) 620, 925
BspT107I GGYRCC 1 cut(s) 751
BspTNI GGTCTC 1 cut(s) 176
BsrBI CCGCTC 1 cut(s) 1000
BsrDI GCAATG 2 cut(s) 1099, 1108
BssECI CCNNGG 3 cut(s) 514, 756, 1090
BssHII GCGCGC 1 cut(s) 404
BssMI GATC 5 cut(s) 79, 322, 489, 520, 804
BssNI GRCGYC 1 cut(s) 123
BssT1I CCWWGG 1 cut(s) 756
Bst4CI ACNGT 5 cut(s) 563, 812, 838, 863, 955
Bst6I CTCTTC 1 cut(s) 353
BstACI GRCGYC 1 cut(s) 123
BstBI TTCGAA 2 cut(s) 620, 925
BstC8I GCNNGC 6 cut(s) 153, 368, 406, 908, 1036, 1106
BstDSI CCRYGG 1 cut(s) 1090
BstF5I GGATG 2 cut(s) 82, 510
BstFNI CGCG 1 cut(s) 406
BstHHI GCGC 2 cut(s) 406, 408
BstKTI GATC 5 cut(s) 82, 325, 492, 523, 807
BstMAI GTCTC 4 cut(s) 71, 129, 176, 663
BstMBI GATC 5 cut(s) 79, 322, 489, 520, 804
BstMCI CGRYCG 1 cut(s) 73
BstMWI GCNNNNNNNGC 6 cut(s) 376, 795, 1000, 1006, 1110, 1126
BstNSI RCATGY 1 cut(s) 984
BstSFI CTRYAG 3 cut(s) 380, 954, 1159
BstSLI GKGCMC 1 cut(s) 756
BstUI CGCG 1 cut(s) 406
BstV1I GCAGC 4 cut(s) 366, 394, 915, 1173
BstZI CGGCCG 1 cut(s) 70
Bsu15I ATCGAT 1 cut(s) 492
BsuRI GGCC 4 cut(s) 72, 1003, 1084, 1096
BsuTUI ATCGAT 1 cut(s) 492
BtgI CCRYGG 1 cut(s) 1090
BtgZI GCGATG 1 cut(s) 1059
BtrI CACGTC 1 cut(s) 591
BtsCI GGATG 2 cut(s) 82, 510
BtsI GCAGTG 1 cut(s) 510
BtsIMutI CAGTG 1 cut(s) 510
BveI ACCTGC 1 cut(s) 938
Cac8I GCNNGC 6 cut(s) 153, 368, 406, 908, 1036, 1106
CaiI CAGNNNCTG 1 cut(s) 953
CfoI GCGC 2 cut(s) 406, 408
Cfr13I GGNCC 3 cut(s) 448, 651, 1094
ClaI ATCGAT 1 cut(s) 492
CseI GACGC 1 cut(s) 112
Csp6I GTAC 5 cut(s) 211, 564, 696, 768, 951
CviAII CATG 3 cut(s) 981, 1150, 1215
CviQI GTAC 5 cut(s) 211, 564, 696, 768, 951
DpnI GATC 5 cut(s) 81, 324, 491, 522, 806
DpnII GATC 5 cut(s) 79, 322, 489, 520, 804
EaeI YGGCCR 2 cut(s) 70, 1001
EagI CGGCCG 1 cut(s) 70
Eam1104I CTCTTC 1 cut(s) 353
EarI CTCTTC 1 cut(s) 353
EciI GGCGGA 1 cut(s) 30
EclXI CGGCCG 1 cut(s) 70
Eco130I CCWWGG 1 cut(s) 756
Eco31I GGTCTC 1 cut(s) 176
Eco47I GGWCC 2 cut(s) 448, 651
Eco52I CGGCCG 1 cut(s) 70
Eco57I CTGAAG 1 cut(s) 417
EcoT14I CCWWGG 1 cut(s) 756
ErhI CCWWGG 1 cut(s) 756
FaeI CATG 3 cut(s) 984, 1153, 1218
FalI AAGNNNNNCTT 2 cut(s) 350, 382
FaqI GGGAC 1 cut(s) 872
FatI CATG 3 cut(s) 980, 1149, 1214
FbaI TGATCA 2 cut(s) 79, 804
Fnu4HI GCNGC 6 cut(s) 73, 380, 383, 904, 1001, 1162
FokI GGATG 2 cut(s) 69, 497
Fsp4HI GCNGC 6 cut(s) 73, 380, 383, 904, 1001, 1162
FspBI CTAG 1 cut(s) 1035
GlaI GCGC 2 cut(s) 405, 407
GluI GCNGC 6 cut(s) 73, 380, 383, 904, 1001, 1162
HaeIII GGCC 4 cut(s) 72, 1003, 1084, 1096
HapII CCGG 3 cut(s) 477, 966, 1193
HgaI GACGC 1 cut(s) 112
HhaI GCGC 2 cut(s) 406, 408
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 3 cut(s) 984, 1153, 1218
Hin6I GCGC 2 cut(s) 404, 406
HinP1I GCGC 2 cut(s) 404, 406
HinfI GANTC 4 cut(s) 458, 853, 988, 1196
HpaII CCGG 3 cut(s) 477, 966, 1193
HphI GGTGA 4 cut(s) 38, 250, 453, 726
Hpy166II GTNNAC 2 cut(s) 508, 823
Hpy188I TCNGA 4 cut(s) 271, 397, 515, 525
Hpy188III TCNNGA 4 cut(s) 142, 572, 857, 1193
Hpy8I GTNNAC 2 cut(s) 508, 823
Hpy99I CGWCG 2 cut(s) 538, 541
HpyAV CCTTC 3 cut(s) 163, 196, 211
HpyCH4III ACNGT 5 cut(s) 563, 812, 838, 863, 955
HpyCH4IV ACGT 1 cut(s) 590
HpyCH4V TGCA 6 cut(s) 382, 418, 503, 1113, 1141, 1161
HpyF10VI GCNNNNNNNGC 6 cut(s) 376, 795, 1000, 1006, 1110, 1126
HpySE526I ACGT 1 cut(s) 590
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 3 cut(s) 984, 1153, 1218
HspAI GCGC 2 cut(s) 404, 406
Kpn2I TCCGGA 1 cut(s) 1192
Ksp22I TGATCA 2 cut(s) 79, 804
Kzo9I GATC 5 cut(s) 79, 322, 489, 520, 804
LmnI GCTCC 3 cut(s) 299, 997, 1195
Lsp1109I GCAGC 4 cut(s) 366, 394, 915, 1173
LweI GCATC 3 cut(s) 484, 490, 798
MaeI CTAG 1 cut(s) 1035
MaeII ACGT 1 cut(s) 590
MalI GATC 5 cut(s) 81, 324, 491, 522, 806
MbiI CCGCTC 1 cut(s) 1000
MboI GATC 5 cut(s) 79, 322, 489, 520, 804
MhlI GDGCHC 1 cut(s) 756
MluCI AATT 7 cut(s) 300, 391, 472, 622, 727, 799, 1136
MroI TCCGGA 1 cut(s) 1192
MseI TTAA 5 cut(s) 42, 111, 785, 897, 1125
MspA1I CMGCKG 1 cut(s) 1164
MspI CCGG 3 cut(s) 477, 966, 1193
Mva1269I GAATGC 1 cut(s) 331
MvnI CGCG 1 cut(s) 406
MwoI GCNNNNNNNGC 6 cut(s) 376, 795, 1000, 1006, 1110, 1126
NdeII GATC 5 cut(s) 79, 322, 489, 520, 804
NheI GCTAGC 1 cut(s) 1034
NlaIII CATG 3 cut(s) 984, 1153, 1218
NlaIV GGNNCC 3 cut(s) 295, 753, 1055
NmeAIII GCCGAG 1 cut(s) 48
NspI RCATGY 1 cut(s) 984
NspV TTCGAA 2 cut(s) 620, 925
PauI GCGCGC 1 cut(s) 404
PciI ACATGT 1 cut(s) 980
PctI GAATGC 1 cut(s) 331
PfeI GAWTC 4 cut(s) 458, 853, 988, 1196
PflMI CCANNNNNTGG 1 cut(s) 1091
PkrI GCNGC 6 cut(s) 74, 381, 384, 905, 1002, 1163
PscI ACATGT 1 cut(s) 980
PspN4I GGNNCC 3 cut(s) 295, 753, 1055
PspPI GGNCC 3 cut(s) 448, 651, 1094
PstI CTGCAG 2 cut(s) 384, 1163
PstNI CAGNNNCTG 1 cut(s) 953
PteI GCGCGC 1 cut(s) 404
RsaI GTAC 5 cut(s) 212, 565, 697, 769, 952
RsaNI GTAC 5 cut(s) 211, 564, 696, 768, 951
SaqAI TTAA 5 cut(s) 42, 111, 785, 897, 1125
SatI GCNGC 6 cut(s) 73, 380, 383, 904, 1001, 1162
Sau3AI GATC 5 cut(s) 79, 322, 489, 520, 804
Sau96I GGNCC 3 cut(s) 448, 651, 1094
ScaI AGTACT 2 cut(s) 697, 769
SduI GDGCHC 1 cut(s) 756
SfaNI GCATC 3 cut(s) 484, 490, 798
SfcI CTRYAG 3 cut(s) 380, 954, 1159
SfuI TTCGAA 2 cut(s) 620, 925
SinI GGWCC 2 cut(s) 448, 651
Sse9I AATT 7 cut(s) 300, 391, 472, 622, 727, 799, 1136
SsiI CCGC 7 cut(s) 15, 73, 155, 329, 796, 1000, 1164
SspMI CTAG 1 cut(s) 1035
StyI CCWWGG 1 cut(s) 756
TaaI ACNGT 5 cut(s) 563, 812, 838, 863, 955
TaiI ACGT 1 cut(s) 593
TaqI TCGA 3 cut(s) 492, 620, 925
TasI AATT 7 cut(s) 300, 391, 472, 622, 727, 799, 1136
TatI WGTACW 3 cut(s) 563, 695, 767
TauI GCSGC 2 cut(s) 75, 1003
TfiI GAWTC 4 cut(s) 458, 853, 988, 1196
Tru1I TTAA 5 cut(s) 42, 111, 785, 897, 1125
Tru9I TTAA 5 cut(s) 42, 111, 785, 897, 1125
TscAI CASTG 1 cut(s) 510
TseI GCWGC 4 cut(s) 379, 382, 903, 1161
TspDTI ATGAA 7 cut(s) 291, 324, 426, 693, 720, 1001, 1166
TspGWI ACGGA 2 cut(s) 461, 1079
TspRI CASTG 1 cut(s) 510
Van91I CCANNNNNTGG 1 cut(s) 1091
VpaK11BI GGWCC 2 cut(s) 448, 651
XapI RAATTY 2 cut(s) 300, 622
XceI RCATGY 1 cut(s) 984
XspI CTAG 1 cut(s) 1035
ZrmI AGTACT 2 cut(s) 697, 769
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.