Prupe.2G001400_v2.0.a1

Domain of unknown function (DUF966)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0014395
Physical Location & Seq
Forward (+)
239573 .. 243040
3468 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G001400.1

Sequence Viewer

Length: 1347 bp
ATGGAAGTGAAAGGTGCATTTGCATTAGGAGGTGAAGTGAGGCGACTTCAAATTATCTACTTCCTCAGTCATCGAATGGGTCGTGTAGATCATCCCCATCTCATTCGAGTCCACCATCTTACCCGCAACGGCGTCTATCTGCGAGACGTTAAGAGGTGGCTTTCGGATTTACGAGGAAAGGACATGCCAGAGGCCTTCGCTTGGTCTTACAAGAGGAGGTACAAGACGGGTTATGTTTGGCAAGACTTGCTGGACGACGACCTCATCACACCAATCTCTGACAATGAATATGTCCTCAAGGGATCCGAGATCATCTATTCCGCTTTTGATAGTCCTCCGAAATCATATGCCGAAAAGGGAAGTTGCTTGTTCACAGAGCAGCCCGTTAAGGATGAGGTTCAGCTACCTACTCTTTCTAAACAAGAGGCCTTTCATTTACACAAACATTCCAAGAATGATATAGATATCCAAACAAAAGTATCCTCTGAAATCAGCGACGAATCACCTCATGTGGGCTCAAAGAGGTCTACTTTGGCCAATGAATTTGATGCAATGAAACCCCAAGCAGATGAGAACCACTCAGCTGACACACTCAACCCCAACTCCAACTATCAACAGAGCAACGAGTCAGAAAACTCTTCTTTATATTCCAAGTTTTTATTGAAGAAGAAGAAGAACAAGTACAAGGACAAAGAAATGGCTCCATCTTCTTTTTCTTCTTCTCCCTCTTCTTGGCATTCATCGTCTCATCAATCACCTGCCGCGCATGGTAGTAAGAGCAATTCAAAAGGCATGTTCCGCAATTTGATCACTTGTGGTGCTGTCGACACAAATGACGCCGTATTAGTCATGTTGAATCGGGCCAATAAAACAACTTCTTCCGACAAGTCTACCAGATGGAGCAGCCAAGACATTATCAAGGCTGATCCTATTTGTAAAGCAGATCACAAGAGGAGGAGTGGGTCTGCTGGAGTTTTTGGGACTTGTTGGAATTCGCAGGGGCAGCCGCAGCAACCCCAAAAGCATACTGGTACTGCTACTAGGAAAAGCTACGATGGAGAAAAGGGTTCAAACAAGCAGCAAAAGGAGCCCGAGTTTAGTGGCCAAAAATTGGCTTCCGCAGCTTACAGGCCCGTCGGTGGGCCGCATTGCTCGCAGTGCAGGAAACAGTTTAAGCCAGAGAAATTGCACTCACACATGAAATCGTGCAGAGGAAAGAAAGCTCTAAACAAGACTGCTGCAGCTTCTGTGGACAAGATACCTCAAATGGGATCAACGACTTCAACTTCTGAACAATCGTCTGCCGCCTATTTTCTTGACCAGCTCAATATGCACAGTACGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF966

Protein Analysis

449

Amino Acids

50.14

Weight (kDa)

9.33

Isoelectric Point (pI)

53.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014395)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05577
fragaria_vesca FvH4_2g11530
malus_domestica MD07G1001300.v1.1 MD07G1001400.v1.1
prunus_persica Prupe.2G001400_v2.0.a1 Prupe.2G001400_v2.0.a1
pyrus_communis pycom07g00180
rosa_chinensis RchiOBHm_Chr1g0313061
rosa_laevigata RLG00000030824
rosa_multiflora Rmu_sc0001606.1_g000003
rosa_roxburghii Rroxscaffold_4G00332510
rosa_rugosa RorugPtG0000700
rosa_samantha Rh1AG000600 Rh1CG002200 Rh1CG004800 Rh1DG000300
rosa_wichuraiana Rw0G008490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 766
Acc36I ACCTGC 1 cut(s) 766
AccB7I CCANNNNNTGG 1 cut(s) 1111
AccI GTMKAC 3 cut(s) 527, 825, 890
AccII CGCG 1 cut(s) 764
AciI CCGC 8 cut(s) 124, 321, 762, 799, 1007, 1119, 1145, 1305
AclWI GGATC 4 cut(s) 297, 310, 920, 1279
AcoI YGGCCR 2 cut(s) 534, 1102
AcsI RAATTY 2 cut(s) 542, 991
AcyI GRCGYC 2 cut(s) 132, 837
AfaI GTAC 4 cut(s) 221, 683, 1033, 1339
AfiI CCNNNNNNNGG 7 cut(s) 201, 512, 732, 1111, 1139, 1140, 1268
AgsI TTSAA 6 cut(s) 50, 664, 786, 856, 1071, 1284
AluBI AGCT 7 cut(s) 403, 584, 1050, 1124, 1223, 1244, 1324
AluI AGCT 7 cut(s) 403, 584, 1050, 1124, 1223, 1244, 1324
Alw26I GTCTC 2 cut(s) 138, 750
AlwI GGATC 4 cut(s) 297, 310, 920, 1279
AlwNI CAGNNNCTG 1 cut(s) 1247
Ama87I CYCGRG 1 cut(s) 1091
AoxI GGCC 7 cut(s) 192, 426, 534, 861, 1102, 1130, 1142
ApeKI GCWGC 8 cut(s) 379, 903, 1003, 1009, 1078, 1121, 1238, 1241
ApoI RAATTY 2 cut(s) 542, 991
AspLEI GCGC 1 cut(s) 766
AspS9I GGNCC 3 cut(s) 861, 1131, 1142
AsuHPI GGTGA 3 cut(s) 44, 495, 747
AvaI CYCGRG 1 cut(s) 1091
BaeI ACNNNNGTAYC 2 cut(s) 462, 495
BalI TGGCCA 2 cut(s) 536, 1104
BamHI GGATCC 1 cut(s) 302
BanII GRGCYC 2 cut(s) 518, 1092
BarI GAAGNNNNNNTAC 2 cut(s) 665, 697
BbvI GCAGC 8 cut(s) 391, 915, 1015, 1021, 1090, 1133, 1225, 1253
BccI CCATC 5 cut(s) 105, 123, 712, 891, 1049
BceAI ACGGC 2 cut(s) 145, 824
BciVI GTATCC 1 cut(s) 490
BclI TGATCA 1 cut(s) 807
BcoDI GTCTC 2 cut(s) 138, 750
BfaI CTAG 1 cut(s) 1041
BfmI CTRYAG 1 cut(s) 1239
BfuAI ACCTGC 1 cut(s) 766
BfuI GTATCC 1 cut(s) 490
BmeT110I CYCGRG 1 cut(s) 1091
BmgT120I GGNCC 3 cut(s) 861, 1131, 1142
BmiI GGNNCC 3 cut(s) 304, 702, 1089
BmsI GCATC 1 cut(s) 538
BplI GAGNNNNNCTC 2 cut(s) 563, 595
BpmI CTGGAG 1 cut(s) 990
BpuEI CTTGAG 1 cut(s) 281
BsaHI GRCGYC 2 cut(s) 132, 837
BsaXI ACNNNNNCTCC 6 cut(s) 21, 51, 587, 617, 1079, 1109
Bsc4I CCNNNNNNNGG 7 cut(s) 201, 512, 732, 1111, 1139, 1140, 1268
Bse1I ACTGG 1 cut(s) 1033
Bse3DI GCAATG 2 cut(s) 558, 1147
BseGI GGATG 2 cut(s) 91, 397
BseLI CCNNNNNNNGG 7 cut(s) 201, 512, 732, 1111, 1139, 1140, 1268
BseMI GCAATG 2 cut(s) 558, 1147
BseMII CTCAG 2 cut(s) 79, 594
BseNI ACTGG 1 cut(s) 1033
BseRI GAGGAG 3 cut(s) 229, 967, 970
BseXI GCAGC 8 cut(s) 391, 915, 1015, 1021, 1090, 1133, 1225, 1253
BsgI GTGCAG 2 cut(s) 1180, 1228
Bsh1236I CGCG 1 cut(s) 764
BshFI GGCC 7 cut(s) 194, 428, 536, 863, 1104, 1132, 1144
BsiHKCI CYCGRG 1 cut(s) 1091
BslFI GGGAC 1 cut(s) 994
BslI CCNNNNNNNGG 7 cut(s) 201, 512, 732, 1111, 1139, 1140, 1268
BsmAI GTCTC 2 cut(s) 138, 750
BsmBI CGTCTC 2 cut(s) 138, 750
BsmFI GGGAC 1 cut(s) 994
BsmI GAATGC 1 cut(s) 736
BsnI GGCC 7 cut(s) 194, 428, 536, 863, 1104, 1132, 1144
BsoBI CYCGRG 1 cut(s) 1091
Bsp1286I GDGCHC 2 cut(s) 518, 1092
Bsp143I GATC 7 cut(s) 88, 302, 309, 807, 925, 943, 1271
BspACI CCGC 8 cut(s) 124, 321, 762, 799, 1007, 1119, 1145, 1305
BspANI GGCC 7 cut(s) 194, 428, 536, 863, 1104, 1132, 1144
BspCNI CTCAG 2 cut(s) 78, 593
BspFNI CGCG 1 cut(s) 764
BspLI GGNNCC 3 cut(s) 304, 702, 1089
BspMAI CTGCAG 1 cut(s) 1243
BspMI ACCTGC 1 cut(s) 766
BspPI GGATC 4 cut(s) 297, 310, 920, 1279
BsrDI GCAATG 2 cut(s) 558, 1147
BsrI ACTGG 1 cut(s) 1033
BssMI GATC 7 cut(s) 88, 302, 309, 807, 925, 943, 1271
BssNI GRCGYC 2 cut(s) 132, 837
Bst4CI ACNGT 2 cut(s) 1170, 1337
Bst6I CTCTTC 2 cut(s) 643, 733
BstACI GRCGYC 2 cut(s) 132, 837
BstAPI GCANNNNNTGC 1 cut(s) 247
BstC8I GCNNGC 1 cut(s) 1154
BstDEI CTNAG 2 cut(s) 65, 580
BstF5I GGATG 2 cut(s) 91, 397
BstFNI CGCG 1 cut(s) 764
BstHHI GCGC 1 cut(s) 766
BstKTI GATC 7 cut(s) 91, 305, 312, 810, 928, 946, 1274
BstMAI GTCTC 2 cut(s) 138, 750
BstMBI GATC 7 cut(s) 88, 302, 309, 807, 925, 943, 1271
BstMWI GCNNNNNNNGC 9 cut(s) 247, 798, 1003, 1009, 1087, 1121, 1153, 1158, 1330
BstNSI RCATGY 2 cut(s) 187, 796
BstSFI CTRYAG 1 cut(s) 1239
BstUI CGCG 1 cut(s) 764
BstV1I GCAGC 8 cut(s) 391, 915, 1015, 1021, 1090, 1133, 1225, 1253
BstX2I RGATCY 1 cut(s) 302
BstYI RGATCY 1 cut(s) 302
BsuI GTATCC 1 cut(s) 490
BsuRI GGCC 7 cut(s) 194, 428, 536, 863, 1104, 1132, 1144
BtsCI GGATG 2 cut(s) 91, 397
BtsI GCAGTG 1 cut(s) 1163
BtsIMutI CAGTG 1 cut(s) 1163
BveI ACCTGC 1 cut(s) 766
Cac8I GCNNGC 1 cut(s) 1154
CaiI CAGNNNCTG 1 cut(s) 1247
CfoI GCGC 1 cut(s) 766
Cfr13I GGNCC 3 cut(s) 861, 1131, 1142
CseI GACGC 2 cut(s) 121, 845
Csp6I GTAC 4 cut(s) 220, 682, 1032, 1338
CviAII CATG 6 cut(s) 184, 509, 767, 793, 850, 1198
CviQI GTAC 4 cut(s) 220, 682, 1032, 1338
DdeI CTNAG 2 cut(s) 65, 580
DpnI GATC 7 cut(s) 90, 304, 311, 809, 927, 945, 1273
DpnII GATC 7 cut(s) 88, 302, 309, 807, 925, 943, 1271
EaeI YGGCCR 2 cut(s) 534, 1102
Eam1104I CTCTTC 2 cut(s) 643, 733
EarI CTCTTC 2 cut(s) 643, 733
Eco147I AGGCCT 2 cut(s) 194, 428
Eco24I GRGCYC 2 cut(s) 518, 1092
Eco32I GATATC 1 cut(s) 466
Eco88I CYCGRG 1 cut(s) 1091
EcoRI GAATTC 1 cut(s) 991
EcoRV GATATC 1 cut(s) 466
EcoT38I GRGCYC 2 cut(s) 518, 1092
Esp3I CGTCTC 2 cut(s) 138, 750
FaeI CATG 6 cut(s) 187, 512, 770, 796, 853, 1201
FaqI GGGAC 1 cut(s) 994
FatI CATG 6 cut(s) 183, 508, 766, 792, 849, 1197
FauI CCCGC 1 cut(s) 131
FauNDI CATATG 1 cut(s) 346
FbaI TGATCA 1 cut(s) 807
FblI GTMKAC 3 cut(s) 527, 825, 890
FokI GGATG 2 cut(s) 78, 404
FriOI GRGCYC 2 cut(s) 518, 1092
FspBI CTAG 1 cut(s) 1041
GlaI GCGC 1 cut(s) 765
GsuI CTGGAG 1 cut(s) 990
HaeIII GGCC 7 cut(s) 194, 428, 536, 863, 1104, 1132, 1144
HgaI GACGC 2 cut(s) 121, 845
HhaI GCGC 1 cut(s) 766
Hin1I GRCGYC 2 cut(s) 132, 837
Hin1II CATG 6 cut(s) 187, 512, 770, 796, 853, 1201
Hin6I GCGC 1 cut(s) 764
HinP1I GCGC 1 cut(s) 764
HincII GTYRAC 1 cut(s) 826
HindII GTYRAC 1 cut(s) 826
HinfI GANTC 4 cut(s) 108, 500, 626, 856
HphI GGTGA 3 cut(s) 44, 495, 747
Hpy166II GTNNAC 6 cut(s) 112, 372, 528, 826, 891, 1252
Hpy188I TCNGA 8 cut(s) 166, 280, 307, 339, 487, 631, 883, 1291
Hpy188III TCNNGA 1 cut(s) 1316
Hpy8I GTNNAC 6 cut(s) 112, 372, 528, 826, 891, 1252
Hpy99I CGWCG 3 cut(s) 260, 500, 1139
HpyAV CCTTC 1 cut(s) 205
HpyCH4III ACNGT 2 cut(s) 1170, 1337
HpyCH4IV ACGT 1 cut(s) 147
HpyCH4V TGCA 8 cut(s) 17, 23, 551, 1161, 1189, 1209, 1241, 1333
HpyF10VI GCNNNNNNNGC 9 cut(s) 247, 798, 1003, 1009, 1087, 1121, 1153, 1158, 1330
HpyF3I CTNAG 2 cut(s) 65, 580
HpySE526I ACGT 1 cut(s) 147
Hsp92I GRCGYC 2 cut(s) 132, 837
Hsp92II CATG 6 cut(s) 187, 512, 770, 796, 853, 1201
HspAI GCGC 1 cut(s) 764
Ksp22I TGATCA 1 cut(s) 807
Kzo9I GATC 7 cut(s) 88, 302, 309, 807, 925, 943, 1271
LmnI GCTCC 3 cut(s) 706, 900, 1087
Lsp1109I GCAGC 8 cut(s) 391, 915, 1015, 1021, 1090, 1133, 1225, 1253
LweI GCATC 1 cut(s) 538
MaeI CTAG 1 cut(s) 1041
MaeII ACGT 1 cut(s) 147
MalI GATC 7 cut(s) 90, 304, 311, 809, 927, 945, 1273
MboI GATC 7 cut(s) 88, 302, 309, 807, 925, 943, 1271
MflI RGATCY 1 cut(s) 302
MhlI GDGCHC 2 cut(s) 518, 1092
MlsI TGGCCA 2 cut(s) 536, 1104
MluCI AATT 7 cut(s) 51, 542, 781, 802, 991, 1109, 1184
MluNI TGGCCA 2 cut(s) 536, 1104
MlyI GAGTC 2 cut(s) 117, 635
MmeI TCCRAC 3 cut(s) 630, 906, 968
Mox20I TGGCCA 2 cut(s) 536, 1104
MscI TGGCCA 2 cut(s) 536, 1104
MseI TTAA 3 cut(s) 150, 387, 1173
Msp20I TGGCCA 2 cut(s) 536, 1104
MspA1I CMGCKG 1 cut(s) 584
Mva1269I GAATGC 1 cut(s) 736
MvnI CGCG 1 cut(s) 764
MwoI GCNNNNNNNGC 9 cut(s) 247, 798, 1003, 1009, 1087, 1121, 1153, 1158, 1330
NdeI CATATG 1 cut(s) 346
NdeII GATC 7 cut(s) 88, 302, 309, 807, 925, 943, 1271
NlaIII CATG 6 cut(s) 187, 512, 770, 796, 853, 1201
NlaIV GGNNCC 3 cut(s) 304, 702, 1089
NspI RCATGY 2 cut(s) 187, 796
PaqCI CACCTGC 1 cut(s) 766
PceI AGGCCT 2 cut(s) 194, 428
PcsI WCGNNNNNNNCGW 1 cut(s) 79
PctI GAATGC 1 cut(s) 736
PfeI GAWTC 2 cut(s) 500, 856
PflMI CCANNNNNTGG 1 cut(s) 1111
PleI GAGTC 2 cut(s) 116, 634
PpsI GAGTC 2 cut(s) 116, 634
PspN4I GGNNCC 3 cut(s) 304, 702, 1089
PspPI GGNCC 3 cut(s) 861, 1131, 1142
PstI CTGCAG 1 cut(s) 1243
PstNI CAGNNNCTG 1 cut(s) 1247
PsuI RGATCY 1 cut(s) 302
PvuII CAGCTG 1 cut(s) 584
RsaI GTAC 4 cut(s) 221, 683, 1033, 1339
RsaNI GTAC 4 cut(s) 220, 682, 1032, 1338
SalI GTCGAC 1 cut(s) 824
SaqAI TTAA 3 cut(s) 150, 387, 1173
Sau3AI GATC 7 cut(s) 88, 302, 309, 807, 925, 943, 1271
Sau96I GGNCC 3 cut(s) 861, 1131, 1142
SchI GAGTC 2 cut(s) 117, 635
SduI GDGCHC 2 cut(s) 518, 1092
SfaNI GCATC 1 cut(s) 538
SfcI CTRYAG 1 cut(s) 1239
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
Sse9I AATT 7 cut(s) 51, 542, 781, 802, 991, 1109, 1184
SseBI AGGCCT 2 cut(s) 194, 428
SsiI CCGC 8 cut(s) 124, 321, 762, 799, 1007, 1119, 1145, 1305
SspMI CTAG 1 cut(s) 1041
StuI AGGCCT 2 cut(s) 194, 428
TaaI ACNGT 2 cut(s) 1170, 1337
TaiI ACGT 1 cut(s) 150
TaqI TCGA 3 cut(s) 73, 106, 825
TasI AATT 7 cut(s) 51, 542, 781, 802, 991, 1109, 1184
TatI WGTACW 1 cut(s) 681
TauI GCSGC 4 cut(s) 764, 1009, 1147, 1307
TfiI GAWTC 2 cut(s) 500, 856
Tru1I TTAA 3 cut(s) 150, 387, 1173
Tru9I TTAA 3 cut(s) 150, 387, 1173
TscAI CASTG 1 cut(s) 1163
TseI GCWGC 8 cut(s) 379, 903, 1003, 1009, 1078, 1121, 1238, 1241
TspDTI ATGAA 6 cut(s) 300, 422, 555, 569, 729, 1214
TspRI CASTG 1 cut(s) 1163
Van91I CCANNNNNTGG 1 cut(s) 1111
XapI RAATTY 2 cut(s) 542, 991
XceI RCATGY 2 cut(s) 187, 796
XcmI CCANNNNNNNNNTGG 1 cut(s) 1025
XmiI GTMKAC 3 cut(s) 527, 825, 890
XspI CTAG 1 cut(s) 1041
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.