AT1G06750
ERF Family

superfamily. Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007046
Physical Location & Seq
Forward (+)
2072979 .. 2076505
3527 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G06750.3

Sequence Viewer

Length: 1560 bp
ATGCAGAGAGAATATTCAGGCAACAGAACTTCGACATACTCTCAGATATTGGTGGTGTCATCGATAGGTTTAATGATAGCAGCCGCAGTGCATTACCGTCTGCGGAGGCTACGTGACACCAAAATCATCCCTCGTCTCAAATCATCTCACAAACACAAAGGCCATGAGAAACTCGAGAGATTCTCTCATTACGTCGCAAGGCAAATGGGATTCAAGGACAGAAGAGAGTGTCCAAATCTCTGCAAGTTAGCAGCAGAATACATAAGTAAATCTGAATGTTGTGAAGAAGACATTTACAGCTTTTTCTCTGAAGAACCTGATGCTGATACCCTCTTCATTAAGCTTGTTGAGGAGTTTGAAAGATGCATTCTCAGCTACTTTGCGTTTCACTGGAGCCACGCCGATCTCATGATTACTCAGGTACTGAGCGCTGATGCTGAGCCAAAGAGGAAGCTGAAACAAATTGTGATGGCAGCTACAAGGGAGCTGAGGATTAAGAGGGTGACTAAGAACTTAAAAGTGGCAAGAGTGTTCAACACTTTGGTAGAGGAAATGAGAGCAATGGGGCTCGCGTCTGTGGATGACTCGCAGTGCACAGAAGTTATGGCACCAGTGGCTCACAAAGACAGAAGTCCAGTGCTACTCCTTATGGGTGGTGGGATGGGTGCAGGGAAAAGCACTGTGCTTAAGGACATACTCAAAGAAGCATTCTGGGCAGGAGCAGACTCTGTGGTGATTGAAGCTGATGCTTTCAAAGAGTCTGATGTCATTTACAGAGCCTTAAGCTCTCGTGGCCATGTCGATATGATCCAGACAGCTGAATTGGTTCATCAGTCATCAACAGATGCAGCTTCATCCCTGCTAGTGACGGCTCTGAACGAAGGAAGGGATGTGATAATGGATGGGACACTCTCTTGGGTACCTTTTGTGGTGCAGACAATAACAATGGCGAGGAACGTACACCGACACCATTACAGAATGGGAGCTGGATACAAGGTTGGTGATGATGGAGTTATAACAGAGAACTATTGGGAACGAATTGGTGAAAGGCAACAGATTCAAGAGGATGGTCGTAGGAGAAAACCGTATAGAATAGAACTAGTTGGAGTTGTGTGCGATGCGTATTTAGCAGTTATAAGAGGCATTAGGAGAGCTATCATGTGCAGAAGAGCAGTTAGGGTGAGATCACAGCTGAGATCTCACAAACGGTTTGCGGAAGCATTTCCTACATATTGCAGCCTTGTCGACAATGCTAGGCTCTACTGCACTAACGCGCTGGAAGGCTCTCCAAAGCTGATAGGTTGGAAAGAAAAGGAAAAGACATTGCTAGTAGATACAGAAGAGATAGATTGTTTGAAGCGAGTAGGAGGGTTAAACGAGAACGCAGGCTCCATTTACGAGCTCTATAGACAACCCAACCCTGCTTGTGAAGCAGGTTCAATCTGGAAAGACATTGTTCTTTCACCTTCAAGGTTTAGCATTCAACAGGAACTCAAATATTCCATTCAGAAAGTTGAAAAAAGATCTAAACAACATCTCATCAACAACACCAAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zeta_toxin

Protein Analysis

519

Amino Acids

59.09

Weight (kDa)

9.12

Isoelectric Point (pI)

46.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zeta_toxin PF06414 207 - 308 9.4e-14 Zeta toxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1016, 1136
Acc36I ACCTGC 1 cut(s) 1424
Acc65I GGTACC 1 cut(s) 919
AccB1I GGYRCC 2 cut(s) 607, 919
AccI GTMKAC 1 cut(s) 1245
AccII CGCG 2 cut(s) 572, 1274
AciI CCGC 3 cut(s) 84, 103, 1214
AclWI GGATC 1 cut(s) 802
AcoI YGGCCR 1 cut(s) 793
AcuI CTGAAG 1 cut(s) 330
AfaI GTAC 3 cut(s) 423, 921, 960
AfeI AGCGCT 1 cut(s) 430
AflII CTTAAG 2 cut(s) 686, 781
AhlI ACTAGT 1 cut(s) 1099
AloI GAACNNNNNNTCC 2 cut(s) 1373, 1405
Alw21I GWGCWC 2 cut(s) 596, 1404
Alw26I GTCTC 1 cut(s) 140
Alw44I GTGCAC 1 cut(s) 592
AlwI GGATC 1 cut(s) 802
AlwNI CAGNNNCTG 2 cut(s) 424, 728
Ama87I CYCGRG 1 cut(s) 173
Aor51HI AGCGCT 1 cut(s) 430
AoxI GGCC 2 cut(s) 160, 793
ApaLI GTGCAC 1 cut(s) 592
ApeKI GCWGC 5 cut(s) 80, 251, 473, 848, 1236
Asp700I GAANNNNTTC 2 cut(s) 825, 1221
Asp718I GGTACC 1 cut(s) 919
AspLEI GCGC 2 cut(s) 431, 1276
AsuHPI GGTGA 6 cut(s) 514, 745, 1013, 1055, 1192, 1455
AvaI CYCGRG 1 cut(s) 173
BaeGI GKGCMC 1 cut(s) 596
BalI TGGCCA 1 cut(s) 795
BanI GGYRCC 2 cut(s) 607, 919
BanII GRGCYC 2 cut(s) 570, 1404
BauI CACGAG 1 cut(s) 789
BbsI GAAGAC 1 cut(s) 294
Bbv12I GWGCWC 2 cut(s) 596, 1404
BbvCI CCTCAGC 1 cut(s) 488
BbvI GCAGC 5 cut(s) 92, 263, 485, 860, 1248
BccI CCATC 5 cut(s) 463, 655, 896, 1001, 1061
BceAI ACGGC 1 cut(s) 885
BcgI CGANNNNNNTGC 2 cut(s) 1225, 1259
BciVI GTATCC 1 cut(s) 983
BcoDI GTCTC 1 cut(s) 140
BcuI ACTAGT 1 cut(s) 1099
BfaI CTAG 4 cut(s) 863, 1100, 1254, 1328
BfmI CTRYAG 1 cut(s) 1405
BfoI RGCGCY 1 cut(s) 432
BfrI CTTAAG 2 cut(s) 686, 781
BfuAI ACCTGC 1 cut(s) 1424
BfuI GTATCC 1 cut(s) 983
BglII AGATCT 2 cut(s) 1196, 1523
BisI GCNGC 6 cut(s) 81, 84, 252, 474, 849, 1237
BlpI GCTNAGC 1 cut(s) 438
BlsI GCNGC 6 cut(s) 82, 85, 253, 475, 850, 1238
BmeT110I CYCGRG 1 cut(s) 173
BmiI GGNNCC 4 cut(s) 395, 609, 921, 1390
BmsI GCATC 6 cut(s) 310, 353, 424, 736, 835, 1108
BoxI GACNNNNGTC 1 cut(s) 630
BpiI GAAGAC 1 cut(s) 294
BplI GAGNNNNNCTC 4 cut(s) 167, 199, 169, 201
BpmI CTGGAG 1 cut(s) 412
Bpu10I CCTNAGC 1 cut(s) 488
Bpu1102I GCTNAGC 1 cut(s) 438
Bsa29I ATCGAT 1 cut(s) 62
BsaAI YACGTR 1 cut(s) 113
BsaXI ACNNNNNCTCC 4 cut(s) 97, 127, 1373, 1403
Bse1I ACTGG 3 cut(s) 395, 611, 635
Bse3DI GCAATG 2 cut(s) 567, 1322
BseCI ATCGAT 1 cut(s) 62
BseGI GGATG 7 cut(s) 126, 586, 666, 854, 895, 907, 1072
BseMI GCAATG 2 cut(s) 567, 1322
BseMII CTCAG 7 cut(s) 56, 385, 416, 429, 431, 479, 1184
BseNI ACTGG 3 cut(s) 395, 611, 635
BseRI GAGGAG 1 cut(s) 365
BseSI GKGCMC 1 cut(s) 596
BseXI GCAGC 5 cut(s) 92, 263, 485, 860, 1248
BsgI GTGCAG 4 cut(s) 687, 953, 1183, 1249
Bsh1236I CGCG 2 cut(s) 572, 1274
BshFI GGCC 2 cut(s) 162, 795
BshNI GGYRCC 2 cut(s) 607, 919
BshVI ATCGAT 1 cut(s) 62
BsiHKAI GWGCWC 2 cut(s) 596, 1404
BsiHKCI CYCGRG 1 cut(s) 173
BslFI GGGAC 1 cut(s) 919
BsmAI GTCTC 1 cut(s) 140
BsmBI CGTCTC 1 cut(s) 140
BsmFI GGGAC 1 cut(s) 919
BsmI GAATGC 3 cut(s) 366, 707, 1479
BsnI GGCC 2 cut(s) 162, 795
BsoBI CYCGRG 1 cut(s) 173
Bsp1286I GDGCHC 3 cut(s) 570, 596, 1404
Bsp143I GATC 5 cut(s) 403, 807, 1184, 1196, 1523
Bsp1720I GCTNAGC 1 cut(s) 438
BspACI CCGC 3 cut(s) 84, 103, 1214
BspANI GGCC 2 cut(s) 162, 795
BspCNI CTCAG 7 cut(s) 55, 384, 417, 430, 430, 480, 1185
BspDI ATCGAT 1 cut(s) 62
BspFNI CGCG 2 cut(s) 572, 1274
BspHI TCATGA 1 cut(s) 408
BspLI GGNNCC 4 cut(s) 395, 609, 921, 1390
BspMI ACCTGC 1 cut(s) 1424
BspPI GGATC 1 cut(s) 802
BspQI GCTCTTC 1 cut(s) 1162
BspT107I GGYRCC 2 cut(s) 607, 919
BspTI CTTAAG 2 cut(s) 686, 781
BsrDI GCAATG 2 cut(s) 567, 1322
BsrI ACTGG 3 cut(s) 395, 611, 635
BssMI GATC 5 cut(s) 403, 807, 1184, 1196, 1523
BssSI CACGAG 1 cut(s) 789
Bst2BI CACGAG 1 cut(s) 789
Bst4CI ACNGT 4 cut(s) 98, 682, 1086, 1209
Bst6I CTCTTC 4 cut(s) 217, 338, 1162, 1335
BstAFI CTTAAG 2 cut(s) 686, 781
BstBAI YACGTR 1 cut(s) 113
BstC8I GCNNGC 2 cut(s) 570, 1387
BstDEI CTNAG 8 cut(s) 42, 371, 417, 425, 438, 488, 507, 1193
BstF5I GGATG 7 cut(s) 126, 586, 666, 854, 895, 907, 1072
BstFNI CGCG 2 cut(s) 572, 1274
BstH2I RGCGCY 1 cut(s) 432
BstHHI GCGC 2 cut(s) 431, 1276
BstKTI GATC 5 cut(s) 406, 810, 1187, 1199, 1526
BstMAI GTCTC 1 cut(s) 140
BstMBI GATC 5 cut(s) 403, 807, 1184, 1196, 1523
BstMWI GCNNNNNNNGC 6 cut(s) 372, 614, 713, 792, 1127, 1430
BstPAI GACNNNNGTC 1 cut(s) 630
BstSFI CTRYAG 1 cut(s) 1405
BstSLI GKGCMC 1 cut(s) 596
BstUI CGCG 2 cut(s) 572, 1274
BstV1I GCAGC 5 cut(s) 92, 263, 485, 860, 1248
BstV2I GAAGAC 1 cut(s) 294
BstX2I RGATCY 2 cut(s) 1196, 1523
BstYI RGATCY 2 cut(s) 1196, 1523
Bsu15I ATCGAT 1 cut(s) 62
BsuI GTATCC 1 cut(s) 983
BsuRI GGCC 2 cut(s) 162, 795
BsuTUI ATCGAT 1 cut(s) 62
BtgZI GCGATG 1 cut(s) 1131
BtsCI GGATG 7 cut(s) 126, 586, 666, 854, 895, 907, 1072
BtsI GCAGTG 2 cut(s) 93, 596
BtsIMutI CAGTG 6 cut(s) 93, 388, 596, 618, 642, 678
BveI ACCTGC 1 cut(s) 1424
Cac8I GCNNGC 2 cut(s) 570, 1387
CaiI CAGNNNCTG 2 cut(s) 424, 728
CciI TCATGA 1 cut(s) 408
CfoI GCGC 2 cut(s) 431, 1276
ClaI ATCGAT 1 cut(s) 62
CseI GACGC 1 cut(s) 561
Csp6I GTAC 3 cut(s) 422, 920, 959
CviAII CATG 4 cut(s) 164, 409, 797, 1159
CviQI GTAC 3 cut(s) 422, 920, 959
DdeI CTNAG 8 cut(s) 42, 371, 417, 425, 438, 488, 507, 1193
DpnI GATC 5 cut(s) 405, 809, 1186, 1198, 1525
DpnII GATC 5 cut(s) 403, 807, 1184, 1196, 1523
EaeI YGGCCR 1 cut(s) 793
Eam1104I CTCTTC 4 cut(s) 217, 338, 1162, 1335
EarI CTCTTC 4 cut(s) 217, 338, 1162, 1335
Ecl136II GAGCTC 1 cut(s) 1402
Eco24I GRGCYC 2 cut(s) 570, 1404
Eco47III AGCGCT 1 cut(s) 430
Eco53kI GAGCTC 1 cut(s) 1402
Eco57I CTGAAG 1 cut(s) 330
Eco88I CYCGRG 1 cut(s) 173
EcoICRI GAGCTC 1 cut(s) 1402
EcoT22I ATGCAT 1 cut(s) 368
EcoT38I GRGCYC 2 cut(s) 570, 1404
Esp3I CGTCTC 1 cut(s) 140
FaeI CATG 4 cut(s) 167, 412, 800, 1162
FaqI GGGAC 1 cut(s) 919
FatI CATG 4 cut(s) 163, 408, 796, 1158
FblI GTMKAC 1 cut(s) 1245
Fnu4HI GCNGC 6 cut(s) 81, 84, 252, 474, 849, 1237
FokI GGATG 7 cut(s) 113, 593, 673, 841, 902, 914, 1079
FriOI GRGCYC 2 cut(s) 570, 1404
Fsp4HI GCNGC 6 cut(s) 81, 84, 252, 474, 849, 1237
FspBI CTAG 4 cut(s) 863, 1100, 1254, 1328
GlaI GCGC 2 cut(s) 430, 1275
GluI GCNGC 6 cut(s) 81, 84, 252, 474, 849, 1237
GsuI CTGGAG 1 cut(s) 412
HaeII RGCGCY 1 cut(s) 432
HaeIII GGCC 2 cut(s) 162, 795
HgaI GACGC 1 cut(s) 561
HhaI GCGC 2 cut(s) 431, 1276
Hin1II CATG 4 cut(s) 167, 412, 800, 1162
Hin6I GCGC 2 cut(s) 429, 1274
HinP1I GCGC 2 cut(s) 429, 1274
HincII GTYRAC 1 cut(s) 1246
HindII GTYRAC 1 cut(s) 1246
HindIII AAGCTT 1 cut(s) 341
HinfI GANTC 6 cut(s) 180, 210, 584, 725, 758, 1057
HphI GGTGA 6 cut(s) 514, 745, 1013, 1055, 1192, 1455
Hpy166II GTNNAC 3 cut(s) 594, 961, 1246
Hpy188I TCNGA 6 cut(s) 45, 274, 310, 763, 876, 1509
Hpy188III TCNNGA 5 cut(s) 175, 409, 811, 1061, 1444
Hpy8I GTNNAC 3 cut(s) 594, 961, 1246
Hpy99I CGWCG 1 cut(s) 197
HpyAV CCTTC 4 cut(s) 875, 879, 1274, 1476
HpyCH4III ACNGT 4 cut(s) 98, 682, 1086, 1209
HpyCH4IV ACGT 3 cut(s) 112, 192, 957
HpyF10VI GCNNNNNNNGC 6 cut(s) 372, 614, 713, 792, 1127, 1430
HpyF3I CTNAG 8 cut(s) 42, 371, 417, 425, 438, 488, 507, 1193
HpySE526I ACGT 3 cut(s) 112, 192, 957
Hsp92II CATG 4 cut(s) 167, 412, 800, 1162
HspAI GCGC 2 cut(s) 429, 1274
KpnI GGTACC 1 cut(s) 923
Kzo9I GATC 5 cut(s) 403, 807, 1184, 1196, 1523
LguI GCTCTTC 1 cut(s) 1162
LmnI GCTCC 5 cut(s) 393, 484, 719, 983, 1394
Lsp1109I GCAGC 5 cut(s) 92, 263, 485, 860, 1248
LweI GCATC 6 cut(s) 310, 353, 424, 736, 835, 1108
MaeI CTAG 4 cut(s) 863, 1100, 1254, 1328
MaeII ACGT 3 cut(s) 112, 192, 957
MaeIII GTNAC 3 cut(s) 113, 502, 865
MalI GATC 5 cut(s) 405, 809, 1186, 1198, 1525
MboI GATC 5 cut(s) 403, 807, 1184, 1196, 1523
MboII GAAGA 7 cut(s) 234, 296, 299, 323, 325, 1179, 1352
MflI RGATCY 2 cut(s) 1196, 1523
MhlI GDGCHC 3 cut(s) 570, 596, 1404
MlsI TGGCCA 1 cut(s) 795
MluCI AATT 3 cut(s) 462, 821, 1038
MluNI TGGCCA 1 cut(s) 795
MlyI GAGTC 3 cut(s) 578, 719, 767
MmeI TCCRAC 2 cut(s) 1084, 1283
Mox20I TGGCCA 1 cut(s) 795
Mph1103I ATGCAT 1 cut(s) 368
MroXI GAANNNNTTC 2 cut(s) 825, 1221
MscI TGGCCA 1 cut(s) 795
MseI TTAA 7 cut(s) 71, 339, 495, 515, 687, 782, 1373
Msp20I TGGCCA 1 cut(s) 795
MspA1I CMGCKG 2 cut(s) 818, 1192
MspCI CTTAAG 2 cut(s) 686, 781
Mva1269I GAATGC 3 cut(s) 366, 707, 1479
MvnI CGCG 2 cut(s) 572, 1274
MwoI GCNNNNNNNGC 6 cut(s) 372, 614, 713, 792, 1127, 1430
NdeII GATC 5 cut(s) 403, 807, 1184, 1196, 1523
NlaIII CATG 4 cut(s) 167, 412, 800, 1162
NlaIV GGNNCC 4 cut(s) 395, 609, 921, 1390
NmuCI GTSAC 3 cut(s) 113, 502, 865
NsiI ATGCAT 1 cut(s) 368
PaeR7I CTCGAG 1 cut(s) 173
PagI TCATGA 1 cut(s) 408
PciSI GCTCTTC 1 cut(s) 1162
PctI GAATGC 3 cut(s) 366, 707, 1479
PdmI GAANNNNTTC 2 cut(s) 825, 1221
PfeI GAWTC 3 cut(s) 180, 210, 1057
PkrI GCNGC 6 cut(s) 82, 85, 253, 475, 850, 1238
PleI GAGTC 3 cut(s) 578, 719, 766
PpsI GAGTC 3 cut(s) 578, 719, 766
Ppu21I YACGTR 1 cut(s) 113
PshAI GACNNNNGTC 1 cut(s) 630
PsiI TTATAA 2 cut(s) 1016, 1136
Psp124BI GAGCTC 1 cut(s) 1404
PspN4I GGNNCC 4 cut(s) 395, 609, 921, 1390
PstNI CAGNNNCTG 2 cut(s) 424, 728
PsuI RGATCY 2 cut(s) 1196, 1523
PvuII CAGCTG 2 cut(s) 818, 1192
RsaI GTAC 3 cut(s) 423, 921, 960
RsaNI GTAC 3 cut(s) 422, 920, 959
SacI GAGCTC 1 cut(s) 1404
SalI GTCGAC 1 cut(s) 1244
SapI GCTCTTC 1 cut(s) 1162
SaqAI TTAA 7 cut(s) 71, 339, 495, 515, 687, 782, 1373
SatI GCNGC 6 cut(s) 81, 84, 252, 474, 849, 1237
Sau3AI GATC 5 cut(s) 403, 807, 1184, 1196, 1523
SchI GAGTC 3 cut(s) 578, 719, 767
SduI GDGCHC 3 cut(s) 570, 596, 1404
SfaNI GCATC 6 cut(s) 310, 353, 424, 736, 835, 1108
SfcI CTRYAG 1 cut(s) 1405
Sfr274I CTCGAG 1 cut(s) 173
SlaI CTCGAG 1 cut(s) 173
SmlI CTYRAG 3 cut(s) 173, 686, 781
SmoI CTYRAG 3 cut(s) 173, 686, 781
SpeI ACTAGT 1 cut(s) 1099
Sse9I AATT 3 cut(s) 462, 821, 1038
SsiI CCGC 3 cut(s) 84, 103, 1214
SspI AATATT 2 cut(s) 14, 1499
SspMI CTAG 4 cut(s) 863, 1100, 1254, 1328
SstI GAGCTC 1 cut(s) 1404
TaaI ACNGT 4 cut(s) 98, 682, 1086, 1209
TaiI ACGT 3 cut(s) 115, 195, 960
TaqI TCGA 5 cut(s) 32, 62, 174, 801, 1245
TasI AATT 3 cut(s) 462, 821, 1038
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 3 cut(s) 180, 210, 1057
Tru1I TTAA 7 cut(s) 71, 339, 495, 515, 687, 782, 1373
Tru9I TTAA 7 cut(s) 71, 339, 495, 515, 687, 782, 1373
TscAI CASTG 6 cut(s) 93, 395, 596, 618, 642, 685
TseFI GTSAC 3 cut(s) 113, 502, 865
TseI GCWGC 5 cut(s) 80, 251, 473, 848, 1236
Tsp45I GTSAC 3 cut(s) 113, 502, 865
TspDTI ATGAA 3 cut(s) 325, 818, 843
TspRI CASTG 6 cut(s) 93, 395, 596, 618, 642, 685
Vha464I CTTAAG 2 cut(s) 686, 781
VneI GTGCAC 1 cut(s) 592
XhoI CTCGAG 1 cut(s) 173
XmiI GTMKAC 1 cut(s) 1245
XmnI GAANNNNTTC 2 cut(s) 825, 1221
XspI CTAG 4 cut(s) 863, 1100, 1254, 1328
Zsp2I ATGCAT 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.