Rw6G027050

Zeta toxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0007046
Physical Location & Seq
Forward (+)
50725304 .. 50728578
3275 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G027050.1

Sequence Viewer

Length: 1539 bp
ATGCAAAGACTAAAAGAAAACAACGGCAAAACCACCTTCGCACATATCCTATCAGCCGCTGCCATCGGCATCATCATTGCCGCGGCGGTGCATTATCGTCAACGAAAGACAAAGTATGAGAAGATCATCCCACGCTTAAGGGTCTCGGACTCCAGCCGCGTTCATAAGATCGAAAGGTTCCCTCATTATGTAGCTAGGCAGCTGGGATTTAAAGATAGAAGAGAATGTCCACACCTCTGCAAATTGGCCACCGAATACATGAGAAAATGCGACGGGTTTGAGGATGATATCTACAGTTTCTTTTCTAGTGAACCGGATGCAGACTCGCTGTTTGTGAAGCTTCTGGAGGAGTTTGAGAGATGCATCCTCAGTTATTTCGCATTTCATTGGAACGAAGCTGATCTGATGATTAGTCAGGTCTTGAGTTGTGATACTGAGCCGAGGAAGAAGCTCAAGAACATTGTCATGGCAGCGACCAGGGAACAGAGATTTGAAAGGGTGACCAAAAATTTGAAGGTGGCTAGAGTTTTCACAACGTTACTGGAGGAGATGAAAGCAATGGGACTGGCCACAGCGGATGACTCAACATGCACAGAGGTGATGGCTCCGGTGGCTCATGCTGATAGAAGTCCAGTCCTCCTCTTTATGGGAGGTGGAATGGGAGCAGGGAAGAGCACCGTGCTAAAGGACATTCTCAAAGAGCCATTCTGGGCAGGAGCAGCAGGGAATGCAGTAGTGATTGAAGCAGATGCCTTCAAAGAATCGGATGTCATATACAGAGCTCTCAGCTCCAGAGGGCATCACGACATGCTTCAAACAGCTGAACTGGTACACCAATCATCTACAGACGCAGCATCATCCCTTCTGGTCACAGCACTTAACGAAGGGCGAGATGTGATCATGGATGGCACACTCTCTTGGGTACCATTTGTTGTTCAGACTGTAACAATGGCCAGAAATGTCCACCGCCGCCGTTATCGCATGGGAGCTGGTTACAGGGTGAATGACGATGGAACTGTCACTGAAAACTATTGGGAACGAATTGAAGATGAAGAACCAGAACAAGTAGAAGGGGGCAGAAAGAGAAAACCATACAGGATAGAGCTTGTTGGAGTTGTGTGTGATGCTTATCTGGCTGTTATAAGAGGAATAAGGCGAGCTATCATGTGTAGAAGAGCTGTTCGAGTAAACGCACAATTGAAATCCCACAAGAGATTTTCAGAGGCATTTAAAACATATTGCCAACTAGTTGACAATGCTAGACTGTATAGCACTAATACGTTGGAAGGACCACCTAAGTTGATAGGATGGAAAGACAAAGACAAGACCTTGCTGGTTGATCCAGATGAAATTGATTGCTTAAAAAGGGTGGCAAGTTTGAATGACAGAGCGACTTCCATATACGAACTCTACAGAAACCCTAATCCAGCTTGTCAAAAGGGATCAGTTTGGAAGGACATTGTCCTGTCGCCTTCAAGGTTAAACATTCAACAGGAGCTGAAGTACTCTATCCAGAAAGTAGAGAGATTGAAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zeta_toxin

Protein Analysis

512

Amino Acids

58.34

Weight (kDa)

9.04

Isoelectric Point (pI)

42.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zeta_toxin PF06414 206 - 309 2.4e-14 Zeta toxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1142
Acc65I GGTACC 1 cut(s) 922
AccB1I GGYRCC 1 cut(s) 922
AccII CGCG 2 cut(s) 83, 159
AciI CCGC 8 cut(s) 57, 81, 83, 86, 157, 575, 967, 970
AclI AACGTT 1 cut(s) 536
AclWI GGATC 2 cut(s) 1334, 1450
AcoI YGGCCR 3 cut(s) 246, 567, 951
AcsI RAATTY 1 cut(s) 508
AcuI CTGAAG 1 cut(s) 1520
AfaI GTAC 3 cut(s) 831, 924, 1505
AfiI CCNNNNNNNGG 1 cut(s) 646
AflII CTTAAG 1 cut(s) 136
AhlI ACTAGT 1 cut(s) 1246
AjnI CCWGG 1 cut(s) 476
AleI CACNNNNGTG 1 cut(s) 596
Alw21I GWGCWC 2 cut(s) 677, 784
Alw26I GTCTC 1 cut(s) 148
AlwI GGATC 2 cut(s) 1334, 1450
AlwNI CAGNNNCTG 2 cut(s) 59, 1498
AoxI GGCC 3 cut(s) 246, 567, 951
ApeKI GCWGC 5 cut(s) 59, 199, 470, 719, 851
ApoI RAATTY 1 cut(s) 508
ArsI GACNNNNNNTTYG 4 cut(s) 314, 346, 493, 525
Asp718I GGTACC 1 cut(s) 922
AspS9I GGNCC 1 cut(s) 1289
AsuHPI GGTGA 3 cut(s) 511, 610, 1012
AvaII GGWCC 1 cut(s) 1289
BalI TGGCCA 3 cut(s) 248, 569, 953
BanI GGYRCC 1 cut(s) 922
BanII GRGCYC 1 cut(s) 784
Bbv12I GWGCWC 2 cut(s) 677, 784
BbvI GCAGC 5 cut(s) 46, 211, 482, 731, 863
BccI CCATC 5 cut(s) 71, 595, 899, 1004, 1302
BceAI ACGGC 2 cut(s) 40, 957
BciT130I CCWGG 1 cut(s) 478
BclI TGATCA 1 cut(s) 897
BcoDI GTCTC 1 cut(s) 148
BcuI ACTAGT 1 cut(s) 1246
BfaI CTAG 5 cut(s) 195, 306, 522, 1247, 1260
BfmI CTRYAG 3 cut(s) 292, 843, 1411
BfrI CTTAAG 1 cut(s) 136
BmcAI AGTACT 1 cut(s) 1505
Bme1390I CCNGG 1 cut(s) 478
Bme18I GGWCC 1 cut(s) 1289
BmgT120I GGNCC 1 cut(s) 1289
BmiI GGNNCC 3 cut(s) 179, 606, 924
BmrFI CCNGG 1 cut(s) 478
BmsI GCATC 8 cut(s) 78, 307, 350, 372, 739, 808, 863, 1114
BpmI CTGGAG 4 cut(s) 136, 365, 563, 775
BpuEI CTTGAG 2 cut(s) 437, 442
BsaBI GATNNNNATC 1 cut(s) 1128
BsaI GGTCTC 1 cut(s) 148
BsaJI CCNNGG 3 cut(s) 81, 440, 477
BsaWI WCCGGW 2 cut(s) 313, 607
BsaXI ACNNNNNCTCC 2 cut(s) 1487, 1517
Bsc4I CCNNNNNNNGG 1 cut(s) 646
Bse1I ACTGG 4 cut(s) 546, 570, 632, 831
Bse3DI GCAATG 2 cut(s) 75, 564
Bse8I GATNNNNATC 1 cut(s) 1128
BseBI CCWGG 1 cut(s) 478
BseDI CCNNGG 3 cut(s) 81, 440, 477
BseGI GGATG 9 cut(s) 126, 289, 322, 363, 583, 772, 857, 910, 1313
BseJI GATNNNNATC 1 cut(s) 1128
BseLI CCNNNNNNNGG 1 cut(s) 646
BseMI GCAATG 2 cut(s) 75, 564
BseMII CTCAG 3 cut(s) 382, 426, 799
BseNI ACTGG 4 cut(s) 546, 570, 632, 831
BseRI GAGGAG 3 cut(s) 362, 560, 629
BseXI GCAGC 5 cut(s) 46, 211, 482, 731, 863
BseYI CCCAGC 1 cut(s) 202
Bsh1236I CGCG 2 cut(s) 83, 159
BshFI GGCC 3 cut(s) 248, 569, 953
BshNI GGYRCC 1 cut(s) 922
BsiHKAI GWGCWC 2 cut(s) 677, 784
BsiSI CCGG 2 cut(s) 314, 608
BslFI GGGAC 1 cut(s) 576
BslI CCNNNNNNNGG 1 cut(s) 646
BsmAI GTCTC 1 cut(s) 148
BsmFI GGGAC 1 cut(s) 576
BsmI GAATGC 1 cut(s) 733
BsnI GGCC 3 cut(s) 248, 569, 953
Bso31I GGTCTC 1 cut(s) 148
Bsp1286I GDGCHC 2 cut(s) 677, 784
Bsp143I GATC 6 cut(s) 123, 168, 400, 897, 1339, 1442
BspACI CCGC 8 cut(s) 57, 81, 83, 86, 157, 575, 967, 970
BspANI GGCC 3 cut(s) 248, 569, 953
BspCNI CTCAG 3 cut(s) 381, 427, 798
BspFNI CGCG 2 cut(s) 83, 159
BspLI GGNNCC 3 cut(s) 179, 606, 924
BspPI GGATC 2 cut(s) 1334, 1450
BspQI GCTCTTC 2 cut(s) 665, 1168
BspT107I GGYRCC 1 cut(s) 922
BspTI CTTAAG 1 cut(s) 136
BspTNI GGTCTC 1 cut(s) 148
BsrDI GCAATG 2 cut(s) 75, 564
BsrI ACTGG 4 cut(s) 546, 570, 632, 831
BssECI CCNNGG 3 cut(s) 81, 440, 477
BssMI GATC 6 cut(s) 123, 168, 400, 897, 1339, 1442
Bst2UI CCWGG 1 cut(s) 478
Bst4CI ACNGT 5 cut(s) 296, 679, 943, 1018, 1266
Bst6I CTCTTC 3 cut(s) 214, 665, 1168
BstAFI CTTAAG 1 cut(s) 136
BstAPI GCANNNNNTGC 1 cut(s) 728
BstC8I GCNNGC 1 cut(s) 1158
BstDEI CTNAG 4 cut(s) 368, 435, 785, 1296
BstDSI CCRYGG 1 cut(s) 81
BstEII GGTNACC 1 cut(s) 499
BstF5I GGATG 9 cut(s) 126, 289, 322, 363, 583, 772, 857, 910, 1313
BstFNI CGCG 2 cut(s) 83, 159
BstKTI GATC 6 cut(s) 126, 171, 403, 900, 1342, 1445
BstMAI GTCTC 1 cut(s) 148
BstMBI GATC 6 cut(s) 123, 168, 400, 897, 1339, 1442
BstMWI GCNNNNNNNGC 5 cut(s) 611, 719, 728, 978, 1133
BstNI CCWGG 1 cut(s) 478
BstNSI RCATGY 2 cut(s) 591, 811
BstPI GGTNACC 1 cut(s) 499
BstSCI CCNGG 1 cut(s) 476
BstSFI CTRYAG 3 cut(s) 292, 843, 1411
BstUI CGCG 2 cut(s) 83, 159
BstV1I GCAGC 5 cut(s) 46, 211, 482, 731, 863
BsuRI GGCC 3 cut(s) 248, 569, 953
BtgI CCRYGG 1 cut(s) 81
BtsCI GGATG 9 cut(s) 126, 289, 322, 363, 583, 772, 857, 910, 1313
BtsIMutI CAGTG 1 cut(s) 1020
Cac8I GCNNGC 1 cut(s) 1158
CaiI CAGNNNCTG 2 cut(s) 59, 1498
Cfr13I GGNCC 1 cut(s) 1289
Cfr42I CCGCGG 1 cut(s) 84
CseI GACGC 1 cut(s) 857
Csp6I GTAC 3 cut(s) 830, 923, 1504
CspCI CAANNNNNGTGG 2 cut(s) 1281, 1316
CviAII CATG 8 cut(s) 259, 466, 588, 617, 808, 901, 982, 1165
CviQI GTAC 3 cut(s) 830, 923, 1504
DdeI CTNAG 4 cut(s) 368, 435, 785, 1296
DpnI GATC 6 cut(s) 125, 170, 402, 899, 1341, 1444
DpnII GATC 6 cut(s) 123, 168, 400, 897, 1339, 1442
DraI TTTAAA 2 cut(s) 211, 1231
EaeI YGGCCR 3 cut(s) 246, 567, 951
Eam1104I CTCTTC 3 cut(s) 214, 665, 1168
EarI CTCTTC 3 cut(s) 214, 665, 1168
Ecl136II GAGCTC 1 cut(s) 782
Eco24I GRGCYC 1 cut(s) 784
Eco31I GGTCTC 1 cut(s) 148
Eco32I GATATC 1 cut(s) 289
Eco47I GGWCC 1 cut(s) 1289
Eco53kI GAGCTC 1 cut(s) 782
Eco57I CTGAAG 1 cut(s) 1520
Eco91I GGTNACC 1 cut(s) 499
EcoICRI GAGCTC 1 cut(s) 782
EcoO65I GGTNACC 1 cut(s) 499
EcoRII CCWGG 1 cut(s) 476
EcoRV GATATC 1 cut(s) 289
EcoT22I ATGCAT 1 cut(s) 365
EcoT38I GRGCYC 1 cut(s) 784
FaeI CATG 8 cut(s) 262, 469, 591, 620, 811, 904, 985, 1168
FaqI GGGAC 1 cut(s) 576
FatI CATG 8 cut(s) 258, 465, 587, 616, 807, 900, 981, 1164
FbaI TGATCA 1 cut(s) 897
FokI GGATG 9 cut(s) 113, 296, 329, 350, 590, 779, 844, 917, 1320
FriOI GRGCYC 1 cut(s) 784
FspBI CTAG 5 cut(s) 195, 306, 522, 1247, 1260
GsaI CCCAGC 1 cut(s) 206
GsuI CTGGAG 4 cut(s) 136, 365, 563, 775
HaeIII GGCC 3 cut(s) 248, 569, 953
HapII CCGG 2 cut(s) 314, 608
HgaI GACGC 1 cut(s) 857
Hin1II CATG 8 cut(s) 262, 469, 591, 620, 811, 904, 985, 1168
HincII GTYRAC 2 cut(s) 101, 1252
HindII GTYRAC 2 cut(s) 101, 1252
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 4 cut(s) 149, 323, 581, 761
HpaII CCGG 2 cut(s) 314, 608
HphI GGTGA 3 cut(s) 511, 610, 1012
Hpy166II GTNNAC 7 cut(s) 101, 230, 311, 832, 964, 1189, 1252
Hpy188I TCNGA 5 cut(s) 148, 405, 766, 939, 1222
Hpy188III TCNNGA 7 cut(s) 344, 421, 454, 792, 803, 1343, 1513
Hpy8I GTNNAC 7 cut(s) 101, 230, 311, 832, 964, 1189, 1252
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 9 cut(s) 46, 508, 763, 872, 878, 1064, 1280, 1447, 1482
HpyCH4III ACNGT 5 cut(s) 296, 679, 943, 1018, 1266
HpyCH4IV ACGT 2 cut(s) 536, 1280
HpyCH4V TGCA 7 cut(s) 4, 91, 240, 320, 363, 591, 731
HpyF10VI GCNNNNNNNGC 5 cut(s) 611, 719, 728, 978, 1133
HpyF3I CTNAG 4 cut(s) 368, 435, 785, 1296
HpySE526I ACGT 2 cut(s) 536, 1280
Hsp92II CATG 8 cut(s) 262, 469, 591, 620, 811, 904, 985, 1168
KpnI GGTACC 1 cut(s) 926
Ksp22I TGATCA 1 cut(s) 897
KspI CCGCGG 1 cut(s) 84
Kzo9I GATC 6 cut(s) 123, 168, 400, 897, 1339, 1442
LguI GCTCTTC 2 cut(s) 665, 1168
LmnI GCTCC 6 cut(s) 610, 662, 716, 794, 986, 1495
Lsp1109I GCAGC 5 cut(s) 46, 211, 482, 731, 863
LweI GCATC 8 cut(s) 78, 307, 350, 372, 739, 808, 863, 1114
MaeI CTAG 5 cut(s) 195, 306, 522, 1247, 1260
MaeII ACGT 2 cut(s) 536, 1280
MaeIII GTNAC 6 cut(s) 499, 537, 868, 943, 992, 1018
MalI GATC 6 cut(s) 125, 170, 402, 899, 1341, 1444
MboI GATC 6 cut(s) 123, 168, 400, 897, 1339, 1442
MboII GAAGA 7 cut(s) 133, 231, 457, 682, 1058, 1064, 1185
MfeI CAATTG 1 cut(s) 1196
MhlI GDGCHC 2 cut(s) 677, 784
MlsI TGGCCA 3 cut(s) 248, 569, 953
MluCI AATT 5 cut(s) 242, 508, 1041, 1196, 1350
MluNI TGGCCA 3 cut(s) 248, 569, 953
MlyI GAGTC 3 cut(s) 143, 317, 575
MmeI TCCRAC 2 cut(s) 1090, 1263
Mox20I TGGCCA 3 cut(s) 248, 569, 953
Mph1103I ATGCAT 1 cut(s) 365
MscI TGGCCA 3 cut(s) 248, 569, 953
MseI TTAA 6 cut(s) 137, 210, 879, 1230, 1361, 1481
MslI CAYNNNNRTG 2 cut(s) 464, 596
Msp20I TGGCCA 3 cut(s) 248, 569, 953
MspA1I CMGCKG 5 cut(s) 59, 83, 202, 575, 821
MspCI CTTAAG 1 cut(s) 136
MspI CCGG 2 cut(s) 314, 608
MspR9I CCNGG 1 cut(s) 478
MunI CAATTG 1 cut(s) 1196
Mva1269I GAATGC 1 cut(s) 733
MvaI CCWGG 1 cut(s) 478
MvnI CGCG 2 cut(s) 83, 159
MwoI GCNNNNNNNGC 5 cut(s) 611, 719, 728, 978, 1133
NdeII GATC 6 cut(s) 123, 168, 400, 897, 1339, 1442
NlaIII CATG 8 cut(s) 262, 469, 591, 620, 811, 904, 985, 1168
NlaIV GGNNCC 3 cut(s) 179, 606, 924
NmeAIII GCCGAG 1 cut(s) 465
NmuCI GTSAC 3 cut(s) 499, 868, 1018
NsiI ATGCAT 1 cut(s) 365
NspI RCATGY 2 cut(s) 591, 811
OliI CACNNNNGTG 1 cut(s) 596
PciSI GCTCTTC 2 cut(s) 665, 1168
PctI GAATGC 1 cut(s) 733
PfeI GAWTC 1 cut(s) 761
PflFI GACNNNGTC 1 cut(s) 1460
PleI GAGTC 3 cut(s) 143, 317, 575
PpsI GAGTC 3 cut(s) 143, 317, 575
PsiI TTATAA 1 cut(s) 1142
Psp124BI GAGCTC 1 cut(s) 784
Psp1406I AACGTT 1 cut(s) 536
Psp6I CCWGG 1 cut(s) 476
PspEI GGTNACC 1 cut(s) 499
PspFI CCCAGC 1 cut(s) 202
PspGI CCWGG 1 cut(s) 476
PspN4I GGNNCC 3 cut(s) 179, 606, 924
PspPI GGNCC 1 cut(s) 1289
PstNI CAGNNNCTG 2 cut(s) 59, 1498
PsyI GACNNNGTC 1 cut(s) 1460
PvuII CAGCTG 2 cut(s) 202, 821
RsaI GTAC 3 cut(s) 831, 924, 1505
RsaNI GTAC 3 cut(s) 830, 923, 1504
RseI CAYNNNNRTG 2 cut(s) 464, 596
SacI GAGCTC 1 cut(s) 784
SacII CCGCGG 1 cut(s) 84
SapI GCTCTTC 2 cut(s) 665, 1168
SaqAI TTAA 6 cut(s) 137, 210, 879, 1230, 1361, 1481
Sau3AI GATC 6 cut(s) 123, 168, 400, 897, 1339, 1442
Sau96I GGNCC 1 cut(s) 1289
ScaI AGTACT 1 cut(s) 1505
SchI GAGTC 3 cut(s) 143, 317, 575
ScrFI CCNGG 1 cut(s) 478
SduI GDGCHC 2 cut(s) 677, 784
SfaNI GCATC 8 cut(s) 78, 307, 350, 372, 739, 808, 863, 1114
SfcI CTRYAG 3 cut(s) 292, 843, 1411
Sfr303I CCGCGG 1 cut(s) 84
SgrBI CCGCGG 1 cut(s) 84
SinI GGWCC 1 cut(s) 1289
SmiMI CAYNNNNRTG 2 cut(s) 464, 596
SmlI CTYRAG 3 cut(s) 136, 421, 452
SmoI CTYRAG 3 cut(s) 136, 421, 452
SpeI ACTAGT 1 cut(s) 1246
Sse9I AATT 5 cut(s) 242, 508, 1041, 1196, 1350
SsiI CCGC 8 cut(s) 57, 81, 83, 86, 157, 575, 967, 970
SspMI CTAG 5 cut(s) 195, 306, 522, 1247, 1260
SstI GAGCTC 1 cut(s) 784
StyD4I CCNGG 1 cut(s) 476
TaaI ACNGT 5 cut(s) 296, 679, 943, 1018, 1266
TaiI ACGT 2 cut(s) 539, 1283
TaqI TCGA 2 cut(s) 171, 1183
TasI AATT 5 cut(s) 242, 508, 1041, 1196, 1350
TatI WGTACW 1 cut(s) 1503
TauI GCSGC 5 cut(s) 59, 83, 86, 159, 972
TfiI GAWTC 1 cut(s) 761
Tru1I TTAA 6 cut(s) 137, 210, 879, 1230, 1361, 1481
Tru9I TTAA 6 cut(s) 137, 210, 879, 1230, 1361, 1481
TscAI CASTG 1 cut(s) 1027
TseFI GTSAC 3 cut(s) 499, 868, 1018
TseI GCWGC 5 cut(s) 59, 199, 470, 719, 851
Tsp45I GTSAC 3 cut(s) 499, 868, 1018
TspDTI ATGAA 5 cut(s) 152, 374, 566, 1065, 1362
TspRI CASTG 1 cut(s) 1027
Tth111I GACNNNGTC 1 cut(s) 1460
Vha464I CTTAAG 1 cut(s) 136
VpaK11BI GGWCC 1 cut(s) 1289
XapI RAATTY 1 cut(s) 508
XceI RCATGY 2 cut(s) 591, 811
XspI CTAG 5 cut(s) 195, 306, 522, 1247, 1260
ZrmI AGTACT 1 cut(s) 1505
Zsp2I ATGCAT 1 cut(s) 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.