RLG00000012319

Zeta toxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0007046
Physical Location & Seq
Reverse (-)
17448160 .. 17451098
2939 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012319

Sequence Viewer

Length: 1536 bp
ATGCAGACAGATGAAAACAACGGCAAAACCACCTTCGCACATATCCTATCAGCCGCTGCCATCGGCATCATCATTGCCGCGGCAGTGCATTATCGTCAACGAAAGACAAAGTATGAGAAGATCATCCCACGCTTAAGGGTCTCGGACTCCAGCCGCGTTCATAAGATTGAAAGGTTCCCTCATTATGTAGCTAGGCAGCTGGGATTTAAAGATAGAAGAGAATGTCCACACCTCTGCAAATTGGCCACCGAATACATGAGAAAATGCGACGGGTTTGAGGATGATATCTACAGTTTCTTTTCTAGTGAACCGGATGCGGATTCGCTGTTTGTGAAGCTTCTGGAGGAGTTTGAGAGATGCATCCTCAGTTATTTCGCATTTCATTGGAACGAAGCTGATCTGATGATTAGTCAGGTCTTGAGTTGTGATACTGAGCCGAGGAAGAAGCTCAAGAACATTGTCATGGCAGCGACCAGGGAACAGCGATTTGAAAGGGTGACCAAAAATTTGAAGGTGGCTAGAGTTTTCACAACGTTACTGGAGGAGATGAAAGCAATGGGACTGGCCACAGCGGATGACTCAACATGTACAGAGGTGATGGCTCCGGTGGCTCATGCTGATAGAAGTCCAGTCCTCCTCTTCATGGGAGGTGGAATGGGAGCAGGGAAGAGCACTGTGCTAAAGGACATTCTCAAAGAGCCATTCTGGGCTGGAGCAGCAGGGAATGCAGTAGTGATTGAAGCAGATGCCTTCAAAGAATCGGATGTCATATACAGAGCTCTCAGCTCCAGAGGGCATCACGACATGCTTCAAACAGCTGAACTGGTACACCAGTCATCTACAGACGCAGCATCATCCCTCCTGGTCACAGCACTTAACGAAGGACGAGATGTGATCATGGATGGCACACTCTCTTGGGTACCATTTGTTGTTCAGACTGTAACAATGGCCAGAAATGTCCACCGCCGCCGTTATCGCATGGGAGCTGGTTACAGGGTGAATGATGATGGAACTGTCACTGAAAACTATTGGGAACGAATTGAAGATGAAGAACCACAACAAGTAGAAGGGGGCAGAAAGAGAAAACCATACAGGATAGAGCTTGTTGGAGTTGTGTGTGATGCTTATCTGGCGGTTATAAGAGGAATAAGGCGAGCTATCATGTGTAGAAGAGCTGTTCGAGTAAACGCACAATTGAAATCCCACAAGAGATTTTCAGAGGCATTTAAAACATATTGCCAACTTGTTGACAATGCTAGACTGTATAGCACTAATACGTTGGAAGGACCACCTAAGTTGATAGGATGGAAAGACAAAGACAAGACCTTGCTGGTTGATCCAGATGAAATTGATTGCTTAAAAAGGGTGGCAAGTTTGAATGACAGAGCGACTTCCATATACGAACTCTACAAAAACCCTAATCCAGCTTGTCAAAAGGGATCAGTTTGGAAGGACATTGTCCTGTCACCTTCAAGGTTAAACATTCAACAGGAGCTGAAGTACTCTATCCAGAAAGTAGAAAGATTGAAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zeta_toxin

Protein Analysis

512

Amino Acids

58.13

Weight (kDa)

8.92

Isoelectric Point (pI)

43.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zeta_toxin PF06414 205 - 308 2.4e-14 Zeta toxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1139
Acc65I GGTACC 1 cut(s) 919
AccB1I GGYRCC 1 cut(s) 919
AccII CGCG 2 cut(s) 80, 156
AciI CCGC 9 cut(s) 54, 78, 80, 154, 317, 572, 964, 967, 1133
AclI AACGTT 1 cut(s) 533
AclWI GGATC 2 cut(s) 1331, 1447
AcoI YGGCCR 3 cut(s) 243, 564, 948
AcsI RAATTY 1 cut(s) 505
AcuI CTGAAG 1 cut(s) 1517
AfaI GTAC 4 cut(s) 589, 828, 921, 1502
AfiI CCNNNNNNNGG 1 cut(s) 643
AflII CTTAAG 1 cut(s) 133
AflIII ACRYGT 1 cut(s) 584
AjnI CCWGG 2 cut(s) 473, 861
Alw21I GWGCWC 2 cut(s) 674, 781
Alw26I GTCTC 1 cut(s) 145
AlwI GGATC 2 cut(s) 1331, 1447
AlwNI CAGNNNCTG 2 cut(s) 56, 1495
AoxI GGCC 3 cut(s) 243, 564, 948
ApeKI GCWGC 5 cut(s) 56, 196, 467, 716, 848
ApoI RAATTY 1 cut(s) 505
ArsI GACNNNNNNTTYG 2 cut(s) 490, 522
Asp718I GGTACC 1 cut(s) 919
AspS9I GGNCC 1 cut(s) 1286
AsuHPI GGTGA 4 cut(s) 508, 607, 1009, 1458
AvaII GGWCC 1 cut(s) 1286
BaeI ACNNNNGTAYC 2 cut(s) 818, 851
BalI TGGCCA 3 cut(s) 245, 566, 950
BanI GGYRCC 1 cut(s) 919
BanII GRGCYC 1 cut(s) 781
Bbv12I GWGCWC 2 cut(s) 674, 781
BbvI GCAGC 5 cut(s) 43, 208, 479, 728, 860
BccI CCATC 5 cut(s) 68, 592, 896, 1001, 1299
BceAI ACGGC 2 cut(s) 37, 954
BciT130I CCWGG 2 cut(s) 475, 863
BclI TGATCA 1 cut(s) 894
BcoDI GTCTC 1 cut(s) 145
BfaI CTAG 4 cut(s) 192, 303, 519, 1257
BfmI CTRYAG 2 cut(s) 289, 840
BfrI CTTAAG 1 cut(s) 133
BmcAI AGTACT 1 cut(s) 1502
Bme1390I CCNGG 2 cut(s) 475, 863
Bme18I GGWCC 1 cut(s) 1286
BmgT120I GGNCC 1 cut(s) 1286
BmiI GGNNCC 3 cut(s) 176, 603, 921
BmrFI CCNGG 2 cut(s) 475, 863
BmsI GCATC 8 cut(s) 75, 304, 347, 369, 736, 805, 860, 1111
BpmI CTGGAG 5 cut(s) 133, 362, 560, 732, 772
BpuEI CTTGAG 2 cut(s) 434, 439
BsaBI GATNNNNATC 1 cut(s) 1125
BsaI GGTCTC 1 cut(s) 145
BsaJI CCNNGG 3 cut(s) 78, 437, 474
BsaWI WCCGGW 2 cut(s) 310, 604
BsaXI ACNNNNNCTCC 2 cut(s) 1484, 1514
Bsc4I CCNNNNNNNGG 1 cut(s) 643
Bse1I ACTGG 5 cut(s) 543, 567, 629, 828, 832
Bse3DI GCAATG 2 cut(s) 72, 561
Bse8I GATNNNNATC 1 cut(s) 1125
BseBI CCWGG 2 cut(s) 475, 863
BseDI CCNNGG 3 cut(s) 78, 437, 474
BseGI GGATG 9 cut(s) 123, 286, 319, 360, 580, 769, 854, 907, 1310
BseJI GATNNNNATC 1 cut(s) 1125
BseLI CCNNNNNNNGG 1 cut(s) 643
BseMI GCAATG 2 cut(s) 72, 561
BseMII CTCAG 3 cut(s) 379, 423, 796
BseNI ACTGG 5 cut(s) 543, 567, 629, 828, 832
BseRI GAGGAG 3 cut(s) 359, 557, 626
BseXI GCAGC 5 cut(s) 43, 208, 479, 728, 860
BseYI CCCAGC 1 cut(s) 199
Bsh1236I CGCG 2 cut(s) 80, 156
BshFI GGCC 3 cut(s) 245, 566, 950
BshNI GGYRCC 1 cut(s) 919
BsiHKAI GWGCWC 2 cut(s) 674, 781
BsiSI CCGG 2 cut(s) 311, 605
BslFI GGGAC 1 cut(s) 573
BslI CCNNNNNNNGG 1 cut(s) 643
BsmAI GTCTC 1 cut(s) 145
BsmFI GGGAC 1 cut(s) 573
BsmI GAATGC 1 cut(s) 730
BsnI GGCC 3 cut(s) 245, 566, 950
Bso31I GGTCTC 1 cut(s) 145
Bsp1286I GDGCHC 2 cut(s) 674, 781
Bsp1407I TGTACA 1 cut(s) 587
Bsp143I GATC 5 cut(s) 120, 397, 894, 1336, 1439
BspACI CCGC 9 cut(s) 54, 78, 80, 154, 317, 572, 964, 967, 1133
BspANI GGCC 3 cut(s) 245, 566, 950
BspCNI CTCAG 3 cut(s) 378, 424, 795
BspFNI CGCG 2 cut(s) 80, 156
BspLI GGNNCC 3 cut(s) 176, 603, 921
BspPI GGATC 2 cut(s) 1331, 1447
BspQI GCTCTTC 2 cut(s) 662, 1165
BspT107I GGYRCC 1 cut(s) 919
BspTI CTTAAG 1 cut(s) 133
BspTNI GGTCTC 1 cut(s) 145
BsrDI GCAATG 2 cut(s) 72, 561
BsrGI TGTACA 1 cut(s) 587
BsrI ACTGG 5 cut(s) 543, 567, 629, 828, 832
BssECI CCNNGG 3 cut(s) 78, 437, 474
BssMI GATC 5 cut(s) 120, 397, 894, 1336, 1439
Bst2UI CCWGG 2 cut(s) 475, 863
Bst4CI ACNGT 5 cut(s) 293, 676, 940, 1015, 1263
Bst6I CTCTTC 4 cut(s) 211, 644, 662, 1165
BstAFI CTTAAG 1 cut(s) 133
BstAPI GCANNNNNTGC 1 cut(s) 725
BstAUI TGTACA 1 cut(s) 587
BstC8I GCNNGC 1 cut(s) 1155
BstDEI CTNAG 4 cut(s) 365, 432, 782, 1293
BstDSI CCRYGG 1 cut(s) 78
BstEII GGTNACC 1 cut(s) 496
BstF5I GGATG 9 cut(s) 123, 286, 319, 360, 580, 769, 854, 907, 1310
BstFNI CGCG 2 cut(s) 80, 156
BstKTI GATC 5 cut(s) 123, 400, 897, 1339, 1442
BstMAI GTCTC 1 cut(s) 145
BstMBI GATC 5 cut(s) 120, 397, 894, 1336, 1439
BstMWI GCNNNNNNNGC 5 cut(s) 608, 716, 725, 975, 1130
BstNI CCWGG 2 cut(s) 475, 863
BstNSI RCATGY 2 cut(s) 588, 808
BstPI GGTNACC 1 cut(s) 496
BstSCI CCNGG 2 cut(s) 473, 861
BstSFI CTRYAG 2 cut(s) 289, 840
BstUI CGCG 2 cut(s) 80, 156
BstV1I GCAGC 5 cut(s) 43, 208, 479, 728, 860
BsuRI GGCC 3 cut(s) 245, 566, 950
BtgI CCRYGG 1 cut(s) 78
BtsCI GGATG 9 cut(s) 123, 286, 319, 360, 580, 769, 854, 907, 1310
BtsI GCAGTG 1 cut(s) 90
BtsIMutI CAGTG 3 cut(s) 90, 672, 1017
Cac8I GCNNGC 1 cut(s) 1155
CaiI CAGNNNCTG 2 cut(s) 56, 1495
Cfr13I GGNCC 1 cut(s) 1286
Cfr42I CCGCGG 1 cut(s) 81
CseI GACGC 1 cut(s) 854
Csp6I GTAC 4 cut(s) 588, 827, 920, 1501
CspCI CAANNNNNGTGG 2 cut(s) 1278, 1313
CviAII CATG 9 cut(s) 256, 463, 585, 614, 643, 805, 898, 979, 1162
CviQI GTAC 4 cut(s) 588, 827, 920, 1501
DdeI CTNAG 4 cut(s) 365, 432, 782, 1293
DpnI GATC 5 cut(s) 122, 399, 896, 1338, 1441
DpnII GATC 5 cut(s) 120, 397, 894, 1336, 1439
DraI TTTAAA 2 cut(s) 208, 1228
EaeI YGGCCR 3 cut(s) 243, 564, 948
Eam1104I CTCTTC 4 cut(s) 211, 644, 662, 1165
EarI CTCTTC 4 cut(s) 211, 644, 662, 1165
Ecl136II GAGCTC 1 cut(s) 779
Eco24I GRGCYC 1 cut(s) 781
Eco31I GGTCTC 1 cut(s) 145
Eco32I GATATC 1 cut(s) 286
Eco47I GGWCC 1 cut(s) 1286
Eco53kI GAGCTC 1 cut(s) 779
Eco57I CTGAAG 1 cut(s) 1517
Eco91I GGTNACC 1 cut(s) 496
EcoICRI GAGCTC 1 cut(s) 779
EcoO65I GGTNACC 1 cut(s) 496
EcoRII CCWGG 2 cut(s) 473, 861
EcoRV GATATC 1 cut(s) 286
EcoT22I ATGCAT 1 cut(s) 362
EcoT38I GRGCYC 1 cut(s) 781
FaeI CATG 9 cut(s) 259, 466, 588, 617, 646, 808, 901, 982, 1165
FaqI GGGAC 1 cut(s) 573
FatI CATG 9 cut(s) 255, 462, 584, 613, 642, 804, 897, 978, 1161
FbaI TGATCA 1 cut(s) 894
FokI GGATG 9 cut(s) 110, 293, 326, 347, 587, 776, 841, 914, 1317
FriOI GRGCYC 1 cut(s) 781
FspBI CTAG 4 cut(s) 192, 303, 519, 1257
GsaI CCCAGC 1 cut(s) 203
GsuI CTGGAG 5 cut(s) 133, 362, 560, 732, 772
HaeIII GGCC 3 cut(s) 245, 566, 950
HapII CCGG 2 cut(s) 311, 605
HgaI GACGC 1 cut(s) 854
Hin1II CATG 9 cut(s) 259, 466, 588, 617, 646, 808, 901, 982, 1165
HincII GTYRAC 2 cut(s) 98, 1249
HindII GTYRAC 2 cut(s) 98, 1249
HindIII AAGCTT 1 cut(s) 335
HinfI GANTC 4 cut(s) 146, 320, 578, 758
HpaII CCGG 2 cut(s) 311, 605
HphI GGTGA 4 cut(s) 508, 607, 1009, 1458
Hpy166II GTNNAC 7 cut(s) 98, 227, 308, 829, 961, 1186, 1249
Hpy188I TCNGA 5 cut(s) 145, 402, 763, 936, 1219
Hpy188III TCNNGA 7 cut(s) 341, 418, 451, 789, 800, 1340, 1510
Hpy8I GTNNAC 7 cut(s) 98, 227, 308, 829, 961, 1186, 1249
Hpy99I CGWCG 1 cut(s) 272
HpyAV CCTTC 8 cut(s) 43, 505, 760, 875, 1061, 1277, 1444, 1479
HpyCH4III ACNGT 5 cut(s) 293, 676, 940, 1015, 1263
HpyCH4IV ACGT 2 cut(s) 533, 1277
HpyCH4V TGCA 5 cut(s) 4, 88, 237, 360, 728
HpyF10VI GCNNNNNNNGC 5 cut(s) 608, 716, 725, 975, 1130
HpyF3I CTNAG 4 cut(s) 365, 432, 782, 1293
HpySE526I ACGT 2 cut(s) 533, 1277
Hsp92II CATG 9 cut(s) 259, 466, 588, 617, 646, 808, 901, 982, 1165
KpnI GGTACC 1 cut(s) 923
Ksp22I TGATCA 1 cut(s) 894
KspI CCGCGG 1 cut(s) 81
Kzo9I GATC 5 cut(s) 120, 397, 894, 1336, 1439
LguI GCTCTTC 2 cut(s) 662, 1165
LmnI GCTCC 6 cut(s) 607, 659, 713, 791, 983, 1492
Lsp1109I GCAGC 5 cut(s) 43, 208, 479, 728, 860
LweI GCATC 8 cut(s) 75, 304, 347, 369, 736, 805, 860, 1111
MaeI CTAG 4 cut(s) 192, 303, 519, 1257
MaeII ACGT 2 cut(s) 533, 1277
MaeIII GTNAC 7 cut(s) 496, 534, 865, 940, 989, 1015, 1464
MalI GATC 5 cut(s) 122, 399, 896, 1338, 1441
MboI GATC 5 cut(s) 120, 397, 894, 1336, 1439
MboII GAAGA 8 cut(s) 130, 228, 454, 631, 679, 1055, 1061, 1182
MfeI CAATTG 1 cut(s) 1193
MhlI GDGCHC 2 cut(s) 674, 781
MlsI TGGCCA 3 cut(s) 245, 566, 950
MluCI AATT 5 cut(s) 239, 505, 1038, 1193, 1347
MluNI TGGCCA 3 cut(s) 245, 566, 950
MlyI GAGTC 2 cut(s) 140, 572
MmeI TCCRAC 2 cut(s) 1087, 1260
Mox20I TGGCCA 3 cut(s) 245, 566, 950
Mph1103I ATGCAT 1 cut(s) 362
MscI TGGCCA 3 cut(s) 245, 566, 950
MseI TTAA 6 cut(s) 134, 207, 876, 1227, 1358, 1478
MslI CAYNNNNRTG 1 cut(s) 461
Msp20I TGGCCA 3 cut(s) 245, 566, 950
MspA1I CMGCKG 5 cut(s) 56, 80, 199, 572, 818
MspCI CTTAAG 1 cut(s) 133
MspI CCGG 2 cut(s) 311, 605
MspR9I CCNGG 2 cut(s) 475, 863
MunI CAATTG 1 cut(s) 1193
Mva1269I GAATGC 1 cut(s) 730
MvaI CCWGG 2 cut(s) 475, 863
MvnI CGCG 2 cut(s) 80, 156
MwoI GCNNNNNNNGC 5 cut(s) 608, 716, 725, 975, 1130
NdeII GATC 5 cut(s) 120, 397, 894, 1336, 1439
NlaIII CATG 9 cut(s) 259, 466, 588, 617, 646, 808, 901, 982, 1165
NlaIV GGNNCC 3 cut(s) 176, 603, 921
NmeAIII GCCGAG 1 cut(s) 462
NmuCI GTSAC 4 cut(s) 496, 865, 1015, 1464
NsiI ATGCAT 1 cut(s) 362
NspI RCATGY 2 cut(s) 588, 808
PciI ACATGT 1 cut(s) 584
PciSI GCTCTTC 2 cut(s) 662, 1165
PctI GAATGC 1 cut(s) 730
PfeI GAWTC 2 cut(s) 320, 758
PflFI GACNNNGTC 1 cut(s) 1457
PleI GAGTC 2 cut(s) 140, 572
PpsI GAGTC 2 cut(s) 140, 572
PscI ACATGT 1 cut(s) 584
PsiI TTATAA 1 cut(s) 1139
Psp124BI GAGCTC 1 cut(s) 781
Psp1406I AACGTT 1 cut(s) 533
Psp6I CCWGG 2 cut(s) 473, 861
PspEI GGTNACC 1 cut(s) 496
PspFI CCCAGC 1 cut(s) 199
PspGI CCWGG 2 cut(s) 473, 861
PspN4I GGNNCC 3 cut(s) 176, 603, 921
PspPI GGNCC 1 cut(s) 1286
PstNI CAGNNNCTG 2 cut(s) 56, 1495
PsyI GACNNNGTC 1 cut(s) 1457
PvuII CAGCTG 2 cut(s) 199, 818
RsaI GTAC 4 cut(s) 589, 828, 921, 1502
RsaNI GTAC 4 cut(s) 588, 827, 920, 1501
RseI CAYNNNNRTG 1 cut(s) 461
SacI GAGCTC 1 cut(s) 781
SacII CCGCGG 1 cut(s) 81
SapI GCTCTTC 2 cut(s) 662, 1165
SaqAI TTAA 6 cut(s) 134, 207, 876, 1227, 1358, 1478
Sau3AI GATC 5 cut(s) 120, 397, 894, 1336, 1439
Sau96I GGNCC 1 cut(s) 1286
ScaI AGTACT 1 cut(s) 1502
SchI GAGTC 2 cut(s) 140, 572
ScrFI CCNGG 2 cut(s) 475, 863
SduI GDGCHC 2 cut(s) 674, 781
SfaNI GCATC 8 cut(s) 75, 304, 347, 369, 736, 805, 860, 1111
SfcI CTRYAG 2 cut(s) 289, 840
Sfr303I CCGCGG 1 cut(s) 81
SgrBI CCGCGG 1 cut(s) 81
SinI GGWCC 1 cut(s) 1286
SmiMI CAYNNNNRTG 1 cut(s) 461
SmlI CTYRAG 3 cut(s) 133, 418, 449
SmoI CTYRAG 3 cut(s) 133, 418, 449
Sse9I AATT 5 cut(s) 239, 505, 1038, 1193, 1347
SsiI CCGC 9 cut(s) 54, 78, 80, 154, 317, 572, 964, 967, 1133
SspMI CTAG 4 cut(s) 192, 303, 519, 1257
SstI GAGCTC 1 cut(s) 781
StyD4I CCNGG 2 cut(s) 473, 861
TaaI ACNGT 5 cut(s) 293, 676, 940, 1015, 1263
TaiI ACGT 2 cut(s) 536, 1280
TaqI TCGA 1 cut(s) 1180
TasI AATT 5 cut(s) 239, 505, 1038, 1193, 1347
TatI WGTACW 2 cut(s) 587, 1500
TauI GCSGC 5 cut(s) 56, 80, 83, 156, 969
TfiI GAWTC 2 cut(s) 320, 758
Tru1I TTAA 6 cut(s) 134, 207, 876, 1227, 1358, 1478
Tru9I TTAA 6 cut(s) 134, 207, 876, 1227, 1358, 1478
TscAI CASTG 3 cut(s) 90, 679, 1024
TseFI GTSAC 4 cut(s) 496, 865, 1015, 1464
TseI GCWGC 5 cut(s) 56, 196, 467, 716, 848
Tsp45I GTSAC 4 cut(s) 496, 865, 1015, 1464
TspDTI ATGAA 7 cut(s) 27, 149, 371, 563, 631, 1062, 1359
TspRI CASTG 3 cut(s) 90, 679, 1024
Tth111I GACNNNGTC 1 cut(s) 1457
Vha464I CTTAAG 1 cut(s) 133
VpaK11BI GGWCC 1 cut(s) 1286
XapI RAATTY 1 cut(s) 505
XceI RCATGY 2 cut(s) 588, 808
XspI CTAG 4 cut(s) 192, 303, 519, 1257
ZrmI AGTACT 1 cut(s) 1502
Zsp2I ATGCAT 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.